FastQCFastQC Report
Thu 26 May 2016
SRR936990_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936990_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586602
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55340.943399442893137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36570.6234209907228411No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24830.4232852939471737No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7990.1362081956761143No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA7540.12853689554416795No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT6660.11353524195280616No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6150.10484110180326695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6040.10296589510434673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52150.037.9090461
GGTATCA31350.035.1685331
TATCAAC64600.030.6030452
ATCAACG65300.030.2749863
TCAACGC66350.029.8675064
CAACGCA66950.029.3893975
AACGCAG68350.028.7849626
GTACATG40300.027.240231
ACGCAGA74750.026.3885087
CGCAGAG74300.026.2969348
TACATGG41400.025.7129272
GCAGAGT76300.024.9222689
GAGTACT38150.024.79448312-13
ACATGGG41600.024.6754043
AGTACTT39200.023.94845812-13
GTACTTT41200.023.06843414-15
CATGGGA27650.022.5154174
ATGGGAT10500.022.1792935
GTCGGGA1954.1945532E-721.9336912
CATGGGG12300.021.6364864
Produced by FastQC (version 0.11.5)