##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936990_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 586602 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.889679885169162 33.0 31.0 34.0 30.0 34.0 2 31.97726056167555 33.0 31.0 34.0 30.0 34.0 3 31.980063143323754 33.0 31.0 34.0 30.0 34.0 4 35.57709145212597 37.0 35.0 37.0 33.0 37.0 5 35.58398198437782 37.0 35.0 37.0 33.0 37.0 6 35.66286681600131 37.0 35.0 37.0 33.0 37.0 7 35.612585705469805 37.0 35.0 37.0 33.0 37.0 8 35.56479521038114 37.0 35.0 37.0 33.0 37.0 9 37.21203984984709 39.0 37.0 39.0 34.0 39.0 10-11 37.057398372320584 39.0 37.0 39.0 33.0 39.0 12-13 36.99509633448233 39.0 37.0 39.0 33.0 39.0 14-15 38.126152825936494 40.0 38.0 41.0 33.0 41.0 16-17 38.08272815298959 40.0 38.0 41.0 33.0 41.0 18-19 38.1189358372457 40.0 38.0 41.0 33.0 41.0 20-21 38.093395522006404 40.0 38.0 41.0 33.0 41.0 22-23 37.97611157138912 40.0 38.0 41.0 33.0 41.0 24-25 37.816838333316284 40.0 37.5 41.0 33.0 41.0 26-27 37.86513428184698 40.0 38.0 41.0 33.0 41.0 28-29 37.79088717733659 40.0 37.5 41.0 33.0 41.0 30-31 37.59222437018626 40.0 37.0 41.0 33.0 41.0 32-33 37.33532787136764 40.0 36.0 41.0 32.0 41.0 34-35 37.14169232290378 40.0 36.0 41.0 31.5 41.0 36-37 36.92862792830573 39.0 35.5 41.0 31.0 41.0 38-39 36.757548559329834 39.0 35.0 41.0 31.0 41.0 40-41 36.542018267922714 39.0 35.0 41.0 30.0 41.0 42-43 36.26383902543803 39.0 35.0 41.0 30.0 41.0 44-45 35.90970709271363 39.0 35.0 41.0 29.5 41.0 46-47 35.386266156610446 39.0 35.0 40.0 26.5 41.0 48-49 34.925203118980164 39.0 35.0 40.0 24.5 41.0 50-51 34.233608988718075 38.0 34.0 39.5 23.5 40.5 52-53 34.425642087821046 38.5 34.5 40.0 24.0 40.5 54-55 34.79413554675913 39.0 35.0 40.5 24.0 41.0 56-57 34.67724112771521 39.0 35.0 41.0 23.0 41.0 58-59 34.449983975506385 38.0 34.5 40.5 22.5 41.0 60-61 34.17410612306129 38.0 34.0 40.0 22.0 41.0 62-63 33.85779199525402 38.0 34.0 40.0 21.5 41.0 64-65 33.52667055345873 37.0 34.0 40.0 19.5 41.0 66-67 33.13551266446415 36.5 33.0 39.5 18.0 41.0 68-69 32.75963515296573 36.0 33.0 39.0 15.5 41.0 70-71 32.32816031994436 35.5 32.5 39.0 12.0 40.5 72-73 31.92933113081783 35.0 32.0 38.5 8.0 40.0 74-75 31.51679162362215 35.0 32.0 37.0 7.0 39.5 76-77 31.081253558630895 35.0 31.0 37.0 3.5 39.0 78-79 30.65295293913079 35.0 31.0 36.0 2.0 39.0 80-81 30.225796195717027 35.0 31.0 36.0 2.0 37.5 82-83 29.82694910689019 34.5 30.0 35.5 2.0 37.0 84-85 29.49343251472037 34.0 30.0 35.0 2.0 37.0 86-87 29.171404120681483 34.0 30.0 35.0 2.0 36.0 88-89 28.82072427301646 34.0 29.0 35.0 2.0 36.0 90-91 28.474678742997806 34.0 29.0 35.0 2.0 36.0 92-93 28.133620410431604 34.0 29.0 35.0 2.0 35.0 94-95 27.747846069396285 34.0 28.0 35.0 2.0 35.0 96-97 27.28914579220664 34.0 27.0 35.0 2.0 35.0 98-99 26.73601521985946 34.0 25.5 35.0 2.0 35.0 100-101 24.67816935503118 31.5 20.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18745929958469176 1101 2 0.10795458855763229 1101 3 0.11260692212042045 1101 4 0.04697688779251763 1101 5 0.04192492453107377 1101 6 0.02031854568832614 1101 7 -0.011516099840811478 1101 8 -0.06463989317252583 1101 9 -0.06704482787647237 1101 10-11 0.004188979166244167 1101 12-13 0.041818393815553634 1101 14-15 0.26722760687377445 1101 16-17 0.38979133966038404 1101 18-19 0.09097201584448555 1101 20-21 0.18968298429155794 1101 22-23 0.27308517349604244 1101 24-25 0.2620909270284173 1101 26-27 0.3262255256130757 1101 28-29 0.3761802009800803 1101 30-31 0.2808133720649906 1101 32-33 0.4003571189048998 1101 34-35 0.3983629459807432 1101 36-37 0.37697299573950716 1101 38-39 0.3314834308178476 1101 40-41 0.14148429682857255 1101 42-43 0.21169898929544928 1101 44-45 0.5569183785113339 1101 46-47 0.6506462864163254 1101 48-49 0.8594052864940593 1101 50-51 0.7791631491142681 1101 52-53 0.8742289876078644 1101 54-55 0.888089607651338 1101 56-57 1.0004062300720662 1101 58-59 0.8126352215531796 1101 60-61 0.9072553256755924 1101 62-63 1.0054731523220397 1101 64-65 1.0436021374757232 1101 66-67 1.074249385176877 1101 68-69 0.965555111888861 1101 70-71 1.1193019718761512 1101 72-73 0.9889039996549229 1101 74-75 0.7825777918604935 1101 76-77 0.8576570120303337 1101 78-79 0.9093184928790272 1101 80-81 0.9108217971393628 1101 82-83 0.6446346458851124 1101 84-85 0.8208829774806468 1101 86-87 0.843470417769673 1101 88-89 1.078097718925207 1101 90-91 1.1330461659015931 1101 92-93 1.0748225135754197 1101 94-95 1.163421207754567 1101 96-97 1.338179621943798 1101 98-99 1.326215320701337 1101 100-101 1.3784923835196032 1102 1 0.3320106679859869 1102 2 0.12676741983607087 1102 3 0.2383115557049038 1102 4 0.15216201019337205 1102 5 0.14422521906806196 1102 6 0.0539962622850112 1102 7 0.11696196426714778 1102 8 0.1936542139687063 1102 9 0.1691865516645521 1102 10-11 0.43307509657540777 1102 12-13 0.3437493819523425 1102 14-15 0.4180250968010881 1102 16-17 0.43812628240275586 1102 18-19 0.2993268163736218 1102 20-21 0.37181854395725367 1102 22-23 0.2796167507068077 1102 24-25 0.4039508993607086 1102 26-27 0.18335238236154083 1102 28-29 0.3666832750017974 1102 30-31 0.5438609369130845 1102 32-33 0.5371406417852427 1102 34-35 0.5107350130976371 1102 36-37 0.29983548359325596 1102 38-39 0.578695318851409 1102 40-41 0.5197070249131102 1102 42-43 0.4838768981131025 1102 44-45 0.5145979840194528 1102 46-47 0.6866321680142704 1102 48-49 0.9229956106663693 1102 50-51 0.684336465818717 1102 52-53 0.7444925573477406 1102 54-55 0.7454568162700994 1102 56-57 0.8536992625922224 1102 58-59 0.7619388368206614 1102 60-61 0.5885157502568674 1102 62-63 0.5219242389001835 1102 64-65 0.7898193199100518 1102 66-67 0.7231470661143149 1102 68-69 0.8306241752127974 1102 70-71 1.0144104783344652 1102 72-73 0.8333158186705703 1102 74-75 0.7746504180830662 1102 76-77 0.9173329910706798 1102 78-79 0.7647053716042578 1102 80-81 0.8838821060853554 1102 82-83 0.8010165329197356 1102 84-85 0.8262960406350679 1102 86-87 0.772539069407788 1102 88-89 0.6885039862364444 1102 90-91 0.8138855206446074 1102 92-93 0.870595166133775 1102 94-95 0.9577202672340874 1102 96-97 0.8942563771330043 1102 98-99 0.916717789302723 1102 100-101 0.9424741119340965 1103 1 0.08359892764763899 1103 2 0.2283604592830102 1103 3 0.12962442562879062 1103 4 0.015404572069357414 1103 5 0.01284930044150201 1103 6 0.10356671150331209 1103 7 -0.03850703641684561 1103 8 0.24359518917829348 1103 9 0.05920776798281935 1103 10-11 0.2034119713961644 1103 12-13 0.07878476347925556 1103 14-15 0.14200768800791508 1103 16-17 0.15154962390989368 1103 18-19 0.1441905480298047 1103 20-21 0.2769735238636244 1103 22-23 0.20537816309260393 1103 24-25 0.1474061135136111 1103 26-27 0.2317979146702953 1103 28-29 0.08379594380416933 1103 30-31 0.07970302762822001 1103 32-33 0.06539843598473283 1103 34-35 0.13670866277124816 1103 36-37 -0.07450951908563752 1103 38-39 0.026732383307368934 1103 40-41 -0.036890820255926826 1103 42-43 0.09173145628678725 1103 44-45 0.328891098238536 1103 46-47 0.35997571304392295 1103 48-49 0.4111091368658961 1103 50-51 0.287935903508469 1103 52-53 0.3589991686430096 1103 54-55 0.39314032482517547 1103 56-57 0.48093603537012797 1103 58-59 0.5924446863144937 1103 60-61 0.5624522109777814 1103 62-63 0.6454205517960361 1103 64-65 0.7992640274279523 1103 66-67 0.6966626093091222 1103 68-69 0.5734298573029832 1103 70-71 0.5289547350704531 1103 72-73 0.5149669269508621 1103 74-75 0.3930742301582093 1103 76-77 0.36066437543730245 1103 78-79 0.3662617289780563 1103 80-81 0.19096293188404445 1103 82-83 0.4336518693906797 1103 84-85 0.14994160140021862 1103 86-87 0.28791886225411645 1103 88-89 0.3698583031977165 1103 90-91 0.5842783448900484 1103 92-93 0.6016014480314311 1103 94-95 0.48869179379375893 1103 96-97 0.44716971184469756 1103 98-99 0.4836358949070778 1103 100-101 0.7926645252410189 1104 1 0.44062952991713544 1104 2 0.26145001392284684 1104 3 0.29242713813079746 1104 4 0.19446125989650653 1104 5 0.28654656937765566 1104 6 0.26381717245541125 1104 7 0.23567593372336404 1104 8 0.23852780973737708 1104 9 0.461810755199906 1104 10-11 0.4980995758064637 1104 12-13 0.2463980834480637 1104 14-15 0.40125766635244986 1104 16-17 0.44235161868783024 1104 18-19 0.5218541705564377 1104 20-21 0.5276634051916602 1104 22-23 0.34533281035596985 1104 24-25 0.4300811206289836 1104 26-27 0.4306291381423435 1104 28-29 0.35011952598976137 1104 30-31 0.6432247016577435 1104 32-33 0.6382242270280329 1104 34-35 0.7714505295963008 1104 36-37 0.7101454165048153 1104 38-39 0.8682839666314166 1104 40-41 0.9054777929279183 1104 42-43 0.7226650544355593 1104 44-45 0.7888346702769908 1104 46-47 0.7970897816731934 1104 48-49 1.0271081963998725 1104 50-51 1.0266626857534646 1104 52-53 1.1884267836954052 1104 54-55 1.1432733512695918 1104 56-57 1.086947283838775 1104 58-59 0.8208093272668293 1104 60-61 1.0630616577115148 1104 62-63 1.071354211817571 1104 64-65 1.0755862484127192 1104 66-67 1.0419544012681357 1104 68-69 0.9548042989012799 1104 70-71 0.815311171694173 1104 72-73 0.9448527098269608 1104 74-75 0.9778940566669796 1104 76-77 0.999926047734391 1104 78-79 0.8667474301821017 1104 80-81 1.027003198679406 1104 82-83 1.0204114070773826 1104 84-85 1.045866970471259 1104 86-87 1.20377808933954 1104 88-89 1.2524810278267893 1104 90-91 1.2502381152183446 1104 92-93 1.1188809644339024 1104 94-95 1.064597301707284 1104 96-97 1.251360972935391 1104 98-99 1.3454662769095265 1104 100-101 1.1722028925424972 1105 1 0.02351264977846057 1105 2 0.256452138525745 1105 3 0.22934835245938956 1105 4 0.22839588957484835 1105 5 0.2137182170577958 1105 6 0.22394208561648554 1105 7 0.12114972017041481 1105 8 0.25627667492963724 1105 9 0.17308227945072474 1105 10-11 0.28485689219692745 1105 12-13 0.2718832011106045 1105 14-15 0.10980093068677377 1105 16-17 0.17150521736046187 1105 18-19 0.14353405697449517 1105 20-21 0.0886833494798509 1105 22-23 0.13255635904985041 1105 24-25 0.13140747986898305 1105 26-27 -0.010794210906304613 1105 28-29 0.15509193987589498 1105 30-31 -0.2965057098407371 1105 32-33 -0.08510947726770723 1105 34-35 0.021673950139522447 1105 36-37 -0.05601168270402468 1105 38-39 -0.2427391814230475 1105 40-41 0.16519700810635385 1105 42-43 0.3493428162507115 1105 44-45 0.14024399020171785 1105 46-47 -0.044823743616326794 1105 48-49 0.3513057372563182 1105 50-51 0.2608563146426661 1105 52-53 0.16411272691792078 1105 54-55 0.21812007634597563 1105 56-57 0.6306340094547451 1105 58-59 0.3195792496345149 1105 60-61 0.42772276668677733 1105 62-63 0.5966901014191563 1105 64-65 0.6615323374571886 1105 66-67 0.7306840573522564 1105 68-69 0.7194977827778999 1105 70-71 0.7508193564739223 1105 72-73 0.6868921937170711 1105 74-75 0.5805638056940445 1105 76-77 0.5527013524294233 1105 78-79 0.537998855015001 1105 80-81 0.7286364450051899 1105 82-83 0.5801287674437567 1105 84-85 0.6805558341796711 1105 86-87 0.7221036324622325 1105 88-89 0.6176623272743917 1105 90-91 0.5904216067165606 1105 92-93 0.5732669204714824 1105 94-95 0.9884589529621479 1105 96-97 1.154512804477985 1105 98-99 1.1328955411224442 1105 100-101 0.9288523937666788 1106 1 0.16768956831847603 1106 2 0.20304931689007333 1106 3 0.06237808767659914 1106 4 0.19363434023276227 1106 5 0.16172203769654914 1106 6 -0.009276071812621467 1106 7 -0.015594698930691209 1106 8 -0.03973670774491467 1106 9 0.20897905957087914 1106 10-11 0.463933675553136 1106 12-13 0.38300924565340466 1106 14-15 0.2975054679562703 1106 16-17 0.49602072174219103 1106 18-19 0.31507786644363023 1106 20-21 0.2320577772974417 1106 22-23 0.293799580286759 1106 24-25 0.38592361058493196 1106 26-27 0.40624912620243236 1106 28-29 0.43374934027571044 1106 30-31 0.39046157985449526 1106 32-33 0.5737184426241413 1106 34-35 0.4052279792953897 1106 36-37 0.4889343220665552 1106 38-39 0.4561088855529576 1106 40-41 0.41344849173554366 1106 42-43 0.5875403307477356 1106 44-45 0.39658431934035576 1106 46-47 0.5776676573367467 1106 48-49 0.5143574985603507 1106 50-51 0.4508926420275685 1106 52-53 0.6092784706115282 1106 54-55 0.677895065776589 1106 56-57 0.7470812851618476 1106 58-59 0.8927533548899831 1106 60-61 0.7879797724960369 1106 62-63 0.852847749742935 1106 64-65 1.0383728466669098 1106 66-67 1.171597252648489 1106 68-69 1.0909596468529372 1106 70-71 0.9263886681848099 1106 72-73 1.2582955999313512 1106 74-75 1.3775362626466183 1106 76-77 1.4001289455835888 1106 78-79 1.223811340987723 1106 80-81 0.9537461839804067 1106 82-83 0.909622324030444 1106 84-85 1.1789586819126008 1106 86-87 1.214733766445562 1106 88-89 1.103024374552188 1106 90-91 0.9853918819382557 1106 92-93 1.169611477366118 1106 94-95 1.098533277956541 1106 96-97 1.0804709653519318 1106 98-99 1.0688293793507562 1106 100-101 0.9383883323024129 1107 1 -0.021009872485667103 1107 2 0.05974765988640485 1107 3 -0.05043538791433022 1107 4 0.04642517353750719 1107 5 -0.23027082655265474 1107 6 -0.1713914102461871 1107 7 0.02498302243196804 1107 8 -0.020624559815310306 1107 9 0.15813340448837465 1107 10-11 0.14585415309953476 1107 12-13 0.012710093023805769 1107 14-15 0.07634522232176977 1107 16-17 -0.010993241000953446 1107 18-19 0.11711395687554216 1107 20-21 0.2325089076964275 1107 22-23 -0.11012336732723327 1107 24-25 0.2327210993495612 1107 26-27 0.2315312971173924 1107 28-29 0.24521971661795305 1107 30-31 0.020364835540796378 1107 32-33 0.07649726865591333 1107 34-35 -0.04434833550100592 1107 36-37 -0.02876268297650597 1107 38-39 -0.24100250763377318 1107 40-41 -0.03737143347630223 1107 42-43 -0.3104698925875411 1107 44-45 -0.2601346075525086 1107 46-47 -0.32759324355179587 1107 48-49 -0.2854555310461606 1107 50-51 -0.07318880158970842 1107 52-53 -0.034179280940847434 1107 54-55 0.2870291698624925 1107 56-57 0.10209003331787159 1107 58-59 0.20847945184013383 1107 60-61 0.17103922565051022 1107 62-63 0.021995919491459404 1107 64-65 0.03213078004370118 1107 66-67 0.12189743447290624 1107 68-69 0.2922272718143475 1107 70-71 0.2933069158090795 1107 72-73 0.242986326885962 1107 74-75 0.10660243987396356 1107 76-77 0.19868807282637846 1107 78-79 0.2207683549504651 1107 80-81 -0.1315706364937128 1107 82-83 -0.01343507073438488 1107 84-85 0.06946607496210433 1107 86-87 0.05759286967380106 1107 88-89 0.29888015005048985 1107 90-91 0.42631095291839216 1107 92-93 0.4558528578594476 1107 94-95 0.4681394394029006 1107 96-97 0.48227377162315577 1107 98-99 0.3569250258566399 1107 100-101 0.7182229750002769 1108 1 -0.06976785376792094 1108 2 -0.14691725745812434 1108 3 0.013178711622099826 1108 4 -0.015522696662792157 1108 5 -0.007634598091179612 1108 6 -0.07559415668194447 1108 7 0.08869333468596352 1108 8 0.2589214910899642 1108 9 -0.08428328404482244 1108 10-11 -0.01566075987167892 1108 12-13 -0.08867843142296294 1108 14-15 -0.1220978809757014 1108 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-1.8823372595148484 2316 94-95 -2.374252967494648 2316 96-97 -2.231613708452194 2316 98-99 -2.1930568608264522 2316 100-101 -2.607468953705709 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1104.0 3 355.0 4 388.0 5 424.0 6 443.0 7 529.0 8 666.0 9 841.0 10 1129.0 11 1479.0 12 2196.0 13 2840.0 14 3307.0 15 4357.0 16 6180.0 17 8544.0 18 7376.0 19 4041.0 20 3656.0 21 3612.0 22 3861.0 23 4100.0 24 4485.0 25 5115.0 26 6004.0 27 6861.0 28 8150.0 29 9940.0 30 12001.0 31 15009.0 32 19143.0 33 25228.0 34 34915.0 35 51413.0 36 78021.0 37 117687.0 38 109954.0 39 21242.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.46253988338958 19.145732328098543 15.095404649068806 21.296323139443075 2 14.901330823118533 20.881678842572697 41.18815697027988 23.028833364028895 3 18.592893705687967 27.095517898735817 30.86521639788155 23.446371997694666 4 11.887609789374947 17.013729001449647 38.70674511810352 32.391916091071884 5 12.6078716678977 38.25638103880907 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20.795388577317794 26.001907786890488 32.13060923870096 21.07209439709075 28-29 20.453123374973785 26.192481531177485 32.27103860167187 21.083356492176858 30-31 20.644496386503477 26.02075522844561 32.20760002114053 21.127148363910383 32-33 20.3698620795472 26.320984707707517 31.95671445863239 21.352438754112892 34-35 20.887491646094464 26.23058143182531 31.7346117650268 21.147315157053423 36-37 21.107867246868487 25.960763922925782 31.7373237125529 21.194045117652824 38-39 20.840316077502067 26.19027729237169 31.70765388320135 21.261752746924895 40-41 20.784447103991706 26.141303791900654 31.85003904078995 21.224210063317685 42-43 20.607792779008545 26.400679189507343 31.332815644543853 21.658712386940262 44-45 20.838415634650513 26.599550456064623 30.912325294560382 21.64970861472448 46-47 21.15693279155383 26.749638373263572 30.232506599634668 21.860922235547932 48-49 21.17042975878649 27.0204476160359 29.737391886927384 22.071730738250228 50-51 21.311480999397347 27.310643934647576 29.540884475688046 21.836990590267032 52-53 21.368257929891676 27.36737484187951 29.25305756438189 22.011309663846923 54-55 20.874728613184605 27.31615381271305 29.437808413481413 22.371309160620932 56-57 21.238667964760502 27.725954431450244 28.901655751652598 22.133721852136652 58-59 21.410530604799284 27.152846267217782 29.136935027827192 22.299688100155738 60-61 21.29083242826672 27.086867639762463 29.69090199194221 21.93139794002861 62-63 21.396409452040782 26.978040713335833 29.82362669212671 21.801923142496676 64-65 21.186018797768 27.245170562552108 29.661295794625946 21.90751484505395 66-67 21.11681894827654 27.437116703010794 29.217865284055357 22.22819906465731 68-69 21.005100696774107 27.39516631613408 29.532718885366037 22.06701410172578 70-71 21.088840164088154 27.405910174352186 29.26332534261757 22.24192431894209 72-73 21.102237620116078 27.303091475765367 29.0544503474756 22.540220556642957 74-75 20.92638043608916 27.789622827290657 28.868294321395172 22.415702415225013 76-77 21.099360163007105 27.40795682698825 29.004267000865337 22.48841600913931 78-79 20.998964030985277 27.306778989013615 29.08881622421268 22.605440755788422 80-81 20.906454811544602 27.683465529633544 28.912920962726695 22.497158696095156 82-83 20.97924933369029 27.70322233002921 28.843068076986693 22.474460259293807 84-85 21.019697974619227 27.396859713524307 28.498636844914042 23.084805466942424 86-87 21.032220333405512 27.389840969968954 28.76709525944896 22.810843437176572 88-89 21.176822619846327 26.976367897304655 28.662779843191483 23.184029639657535 90-91 20.817114651799187 26.965806501112503 29.1351457336982 23.08193311339011 92-93 21.013591974749723 27.293592452199594 28.883926820576043 22.80888875247464 94-95 20.65768923868046 27.382576196744118 28.668236409392538 23.29149815518288 96-97 20.54483705962955 27.45698082608069 28.532620049096558 23.4655620651932 98-99 20.692637074649216 27.452965270925667 28.26407502701283 23.590322627412284 100-101 20.772225799710863 27.38331108656622 28.425995027527705 23.418468086195208 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 371.0 1 268.5 2 221.5 3 272.0 4 237.5 5 245.0 6 419.5 7 903.5 8 1595.5 9 1826.0 10 1540.5 11 1299.0 12 1318.0 13 1401.0 14 1429.5 15 1446.0 16 1476.0 17 1516.5 18 1600.0 19 1709.5 20 1777.0 21 1998.5 22 2297.5 23 2615.5 24 2973.0 25 3480.0 26 4352.0 27 5280.5 28 5869.5 29 6637.0 30 7634.0 31 8751.0 32 10072.0 33 11294.5 34 12577.0 35 13518.5 36 14767.5 37 16289.0 38 16856.5 39 16868.5 40 17076.5 41 17791.5 42 18322.5 43 18713.5 44 19550.0 45 20466.0 46 20688.5 47 21207.5 48 24534.0 49 24487.0 50 20778.5 51 19108.0 52 18198.0 53 17871.0 54 17228.5 55 15756.0 56 14284.0 57 12923.0 58 11804.5 59 10646.0 60 9099.5 61 7608.0 62 6114.5 63 4503.0 64 3227.5 65 2404.0 66 1664.5 67 1192.5 68 823.0 69 492.5 70 339.5 71 242.0 72 165.0 73 134.5 74 101.5 75 63.0 76 54.5 77 39.0 78 21.5 79 12.0 80 10.5 81 8.0 82 4.0 83 4.0 84 2.5 85 2.0 86 3.5 87 3.5 88 3.5 89 2.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03494703393442232 2 0.033753720580563994 3 0.02318437373210456 4 0.042959280738899625 5 0.06358655442702207 6 0.06154087439183638 7 0.06307513441822564 8 0.06477986778088039 9 0.06119992771930542 10-11 0.06887122785125178 12-13 0.07014977787324285 14-15 0.06230800440503101 16-17 0.05242055090163347 18-19 0.041169310708112146 20-21 0.04815871749499661 22-23 0.026934787129945004 24-25 0.013893576905636189 26-27 0.009972690171530272 28-29 0.012444553547379653 30-31 0.00869414014953921 32-33 0.005455146760495191 34-35 0.008523666813273736 36-37 0.0041765967385041305 38-39 0.0063075134418225645 40-41 0.006137040105557091 42-43 0.003494703393442232 44-45 0.0012785500219910603 46-47 0.002812810048380333 48-49 9.37603349460111E-4 50-51 0.004858490083566029 52-53 0.004091360070371393 54-55 0.005369910092362453 56-57 0.017217806962812946 58-59 0.0060518034374243525 60-61 0.01517212692762725 62-63 0.010569346848459431 64-65 0.007330353459415412 66-67 0.013467393564972502 68-69 0.01934872366613138 70-71 0.015001653591361776 72-73 0.014660706918830825 74-75 0.015939256940821885 76-77 0.021223930365051602 78-79 0.03315706390363483 80-81 0.02787239047940512 82-83 0.027787153811272378 84-85 0.016194966945220098 86-87 0.018581593652936743 88-89 0.015427836932025462 90-91 0.014831180255096301 92-93 0.02608242044861763 94-95 0.016024493608954624 96-97 0.03520274393882053 98-99 0.052250077565368 100-101 0.06384226443142028 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 586602.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.85913855374125 #Duplication Level Percentage of deduplicated Percentage of total 1 82.37724807683607 51.78162850537753 2 10.437468054773703 13.121805012105368 3 3.028300274796441 5.710690396672867 4 1.2898426982432205 3.2431360352560827 5 0.6993361715366835 2.197983465113367 6 0.4369268951883662 1.6478908945500896 7 0.320694667246793 1.4111013365338476 8 0.21591733069732374 1.0857901925165634 9 0.15309931660102635 0.8661322039236313 >10 0.9569303615743686 10.993204661339016 >50 0.059508774551929745 2.5839659417990566 >100 0.021738354245347816 2.4886547507938688 >500 0.002173835424532301 0.8740182813866848 >1k 5.434588561330753E-4 1.04875361495295 >5k 2.7172942806653763E-4 0.945244707679092 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5534 0.943399442893137 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3657 0.6234209907228411 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2483 0.4232852939471737 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 799 0.1362081956761143 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 754 0.12853689554416795 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 666 0.11353524195280616 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 615 0.10484110180326695 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 604 0.10296589510434673 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 1.7047333626547473E-4 0.0 0.0 0.0 0.0 12-13 1.7047333626547473E-4 0.0 0.0 0.0 0.0 14-15 1.7047333626547473E-4 0.0 0.0 1.7047333626547473E-4 0.0 16-17 1.7047333626547473E-4 0.0 0.0 3.4094667253094945E-4 0.0 18-19 1.7047333626547473E-4 0.0 0.0 3.4094667253094945E-4 0.0 20-21 1.7047333626547473E-4 0.0 0.0 5.114200087964242E-4 0.0 22-23 1.7047333626547473E-4 0.0 0.0 7.671300131946362E-4 0.0 24-25 1.7047333626547473E-4 0.0 0.0 0.0016194966945220098 0.0 26-27 2.557100043982121E-4 0.0 0.0 0.003068520052778545 0.0 28-29 3.4094667253094945E-4 0.0 0.0 0.0046880167473005545 0.0 30-31 3.4094667253094945E-4 0.0 0.0 0.008012246804477312 0.0 32-33 3.4094667253094945E-4 0.0 0.0 0.011506950197919544 0.0 34-35 3.4094667253094945E-4 0.0 0.0 0.014234523578167138 0.0 36-37 3.4094667253094945E-4 0.0 0.0 0.01704733362654747 0.0 38-39 3.4094667253094945E-4 0.0 0.0 0.021223930365051602 0.0 40-41 3.4094667253094945E-4 0.0 0.0 0.02582671044421942 0.0 42-43 3.4094667253094945E-4 0.0 0.0 0.037930317319068124 0.0 44-45 5.114200087964242E-4 0.0 0.0 0.05250578756976621 0.0 46-47 6.818933450618989E-4 0.0 0.0 0.07083167121830475 0.0 48-49 6.818933450618989E-4 0.0 0.0 0.1122566919308151 0.0 50-51 6.818933450618989E-4 0.0 0.0 0.15001653591361774 0.0 52-53 6.818933450618989E-4 0.0 0.0 0.19195297663492455 0.0 54-55 6.818933450618989E-4 0.0 0.0 0.2756553847412726 0.0 56-57 6.818933450618989E-4 0.0 0.0 0.35697116613990404 0.0 58-59 6.818933450618989E-4 0.0 0.0 0.448515347714464 0.0 60-61 7.671300131946362E-4 0.0 0.0 0.5566806795749077 0.0 62-63 8.523666813273736E-4 0.0 0.0 0.7056743754709326 0.0 64-65 8.523666813273736E-4 0.0 0.0 0.7911667536080682 0.0 66-67 8.523666813273736E-4 0.0 0.0 0.9393933194908983 0.0 68-69 8.523666813273736E-4 0.0 0.0 1.1517178598095472 0.0 70-71 8.523666813273736E-4 0.0 0.0 1.2727539285580343 0.0 72-73 8.523666813273736E-4 0.0 0.0 1.4771514587403385 0.0 74-75 8.523666813273736E-4 0.0 0.0 1.7396803965891694 0.0 76-77 8.523666813273736E-4 0.0 0.0 1.9072556861381311 0.0 78-79 8.523666813273736E-4 0.0 0.0 2.05667556537482 0.0 80-81 8.523666813273736E-4 0.0 0.0 2.305907582994944 0.0 82-83 8.523666813273736E-4 0.0 0.0 2.631341181925735 0.0 84-85 8.523666813273736E-4 0.0 0.0 3.020531808619814 0.0 86-87 8.523666813273736E-4 0.0 0.0 3.5905094084234284 0.0 88-89 8.523666813273736E-4 0.0 0.0 4.179920968561308 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5215 0.0 37.909046 1 GGTATCA 3135 0.0 35.168533 1 TATCAAC 6460 0.0 30.603045 2 ATCAACG 6530 0.0 30.274986 3 TCAACGC 6635 0.0 29.867506 4 CAACGCA 6695 0.0 29.389397 5 AACGCAG 6835 0.0 28.784962 6 GTACATG 4030 0.0 27.24023 1 ACGCAGA 7475 0.0 26.388508 7 CGCAGAG 7430 0.0 26.296934 8 TACATGG 4140 0.0 25.712927 2 GCAGAGT 7630 0.0 24.922268 9 GAGTACT 3815 0.0 24.794483 12-13 ACATGGG 4160 0.0 24.675404 3 AGTACTT 3920 0.0 23.948458 12-13 GTACTTT 4120 0.0 23.068434 14-15 CATGGGA 2765 0.0 22.515417 4 ATGGGAT 1050 0.0 22.179293 5 GTCGGGA 195 4.1945532E-7 21.933691 2 CATGGGG 1230 0.0 21.636486 4 >>END_MODULE