FastQCFastQC Report
Thu 26 May 2016
SRR936990_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936990_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586602
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57150.974255116757188No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42960.7323534525964794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31100.5301720757856264No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11970.20405658350977326No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10980.18717972321949122No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10230.17439422299958063No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA7850.13382156896839767No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7590.12938926222549532No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.11899038871330135No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6450.10995530189123119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC1107.0043825E-834.5444953
CCCTATA1151.0344047E-733.0453762
GGTATCA44500.029.0477581
GTATCAA63950.028.6103971
GTACACG1109.437771E-525.9216291
CTTAGAC950.001226036924.9993063
GTGTAGG2101.2551027E-924.8929941
AGTACTT42800.023.46789412-13
GAGTACT41900.023.29191812-13
TCAACGC79550.023.0438294
TATCAAC79200.023.0316242
ATCAACG79200.023.0296613
CAACGCA80750.022.701385
GTACATG42000.022.5168461
GTACTTT43850.022.25613414-15
AACGCAG82950.022.0992956
GCGGTAT1100.00286764121.6013581
ACATGGG41550.021.491583
TACATGG42700.021.359542
TACTTTT47150.021.35313414-15
Produced by FastQC (version 0.11.5)