##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936990_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 586602 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23655050613534 33.0 31.0 34.0 31.0 34.0 2 32.290466108196014 33.0 31.0 34.0 30.0 34.0 3 32.40696076726639 34.0 31.0 34.0 31.0 34.0 4 36.09045997115591 37.0 35.0 37.0 35.0 37.0 5 36.017219511696176 37.0 35.0 37.0 35.0 37.0 6 36.06800692803638 37.0 35.0 37.0 35.0 37.0 7 36.02300878619575 37.0 35.0 37.0 35.0 37.0 8 36.004566980678554 37.0 35.0 37.0 35.0 37.0 9 37.700284008578215 39.0 37.0 39.0 35.0 39.0 10-11 37.60188509415242 39.0 37.0 39.0 35.0 39.0 12-13 37.52307441843021 39.0 37.0 39.0 35.0 39.0 14-15 38.69255304277858 40.0 38.0 41.0 34.5 41.0 16-17 38.6426819206208 40.0 38.0 41.0 34.5 41.0 18-19 38.728545078264304 40.0 38.0 41.0 34.5 41.0 20-21 38.816928684184504 40.0 38.0 41.0 35.0 41.0 22-23 38.75987040616977 40.0 38.0 41.0 35.0 41.0 24-25 38.66127885687399 40.0 38.0 41.0 34.0 41.0 26-27 38.457041571627784 40.0 38.0 41.0 34.0 41.0 28-29 38.288907981902554 40.0 38.0 41.0 34.0 41.0 30-31 38.18452545337384 40.0 37.5 41.0 34.0 41.0 32-33 38.02750331570639 40.0 37.0 41.0 33.5 41.0 34-35 37.866003695861934 40.0 37.0 41.0 33.0 41.0 36-37 37.6687830931364 40.0 36.5 41.0 33.0 41.0 38-39 37.50206613683554 40.0 36.0 41.0 33.0 41.0 40-41 37.24756734549149 40.0 36.0 41.0 32.5 41.0 42-43 36.960290793417 40.0 36.0 41.0 31.5 41.0 44-45 36.57922236882929 40.0 35.5 41.0 31.0 41.0 46-47 36.04342382058022 39.0 35.0 41.0 29.5 41.0 48-49 35.52579176341028 39.0 35.0 41.0 27.5 41.0 50-51 35.232335552896174 39.0 35.0 40.5 26.0 41.0 52-53 34.982182126893534 39.0 35.0 40.0 25.5 41.0 54-55 34.688822233814406 38.0 34.5 40.0 24.0 41.0 56-57 34.4305738814392 38.0 34.0 40.0 23.5 41.0 58-59 34.09779202934869 38.0 34.0 40.0 23.0 41.0 60-61 33.76034091257786 37.5 34.0 40.0 22.0 41.0 62-63 33.418546987565676 37.0 33.0 40.0 21.5 41.0 64-65 33.00116433288669 36.5 33.0 39.0 19.5 41.0 66-67 32.575234997494036 36.0 32.0 39.0 18.0 40.0 68-69 32.13030896587465 35.0 32.0 38.5 15.0 40.0 70-71 31.727656059815683 35.0 31.0 38.0 12.0 40.0 72-73 31.343701521645002 35.0 31.0 37.0 7.5 39.5 74-75 30.87510441491846 35.0 31.0 37.0 7.0 39.0 76-77 29.425844098724518 33.5 28.5 35.5 2.0 37.5 78-79 30.077993256074816 34.0 30.0 36.0 2.0 38.0 80-81 29.663652698081492 34.0 30.0 35.5 2.0 37.0 82-83 29.579602524369164 34.0 30.0 35.0 2.0 37.0 84-85 29.303198761681685 34.0 30.0 35.0 2.0 36.5 86-87 28.944856989918208 34.0 29.0 35.0 2.0 36.0 88-89 28.593123617035058 34.0 29.0 35.0 2.0 36.0 90-91 28.174732612572065 34.0 29.0 35.0 2.0 35.0 92-93 27.801594607587425 34.0 27.5 35.0 2.0 35.0 94-95 27.339063794531896 33.0 27.0 35.0 2.0 35.0 96-97 26.765460226865915 33.0 25.5 35.0 2.0 35.0 98-99 26.066811909949166 33.0 24.5 35.0 2.0 35.0 100-101 23.153797634511985 29.5 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19867167194329483 1101 2 0.0614774234276112 1101 3 0.09193464978844901 1101 4 0.11359224401365253 1101 5 0.14614756118818661 1101 6 0.09020614242434277 1101 7 0.05966488804326531 1101 8 0.08254900810436538 1101 9 0.07193349890921041 1101 10-11 0.12772017578263473 1101 12-13 0.009093097162477193 1101 14-15 0.12235489363568064 1101 16-17 0.21799968646539014 1101 18-19 0.10033957092837653 1101 20-21 0.13144578564988763 1101 22-23 0.12382005524259654 1101 24-25 0.05755240234325498 1101 26-27 -0.01472605825581752 1101 28-29 0.04547900564637786 1101 30-31 -0.017252947473728852 1101 32-33 0.13419343903923675 1101 34-35 0.22436247982727764 1101 36-37 0.20668390608155107 1101 38-39 0.1551074356689952 1101 40-41 0.3115925305894862 1101 42-43 0.2667603386422499 1101 44-45 0.30696258205946236 1101 46-47 0.44511895830103043 1101 48-49 0.4359026743394736 1101 50-51 0.49559261494714946 1101 52-53 0.43609767081289164 1101 54-55 0.5961808544570175 1101 56-57 0.7782710004688056 1101 58-59 0.6728580137097211 1101 60-61 0.4308054309502367 1101 62-63 0.5246245709684985 1101 64-65 0.6136419238374415 1101 66-67 0.3574041758635076 1101 68-69 0.4231923454672675 1101 70-71 0.5865938376335826 1101 72-73 0.7019467181028389 1101 74-75 0.7697683874142349 1101 76-77 0.6121354229108356 1101 78-79 0.6506688229569804 1101 80-81 0.999095384437279 1101 82-83 0.9590030925079489 1101 84-85 0.5930845523305344 1101 86-87 0.5600787964061702 1101 88-89 0.43566121392300516 1101 90-91 0.42574373086902284 1101 92-93 0.4272415457578731 1101 94-95 0.36423362814923266 1101 96-97 0.5326746875951898 1101 98-99 0.7177732108772581 1101 100-101 0.7764160492679437 1102 1 0.23500960546208916 1102 2 0.22960446482928631 1102 3 0.10163106176487702 1102 4 0.12572305112976068 1102 5 0.13111255275771327 1102 6 0.18025757288122435 1102 7 0.15485209914425724 1102 8 0.1608413595266498 1102 9 0.14903135602395423 1102 10-11 0.23575955232077916 1102 12-13 0.23628282681203672 1102 14-15 0.18612139512350012 1102 16-17 0.2668961565621615 1102 18-19 0.2514040512204332 1102 20-21 0.19216823372752145 1102 22-23 0.18201457073984528 1102 24-25 0.26797622684402 1102 26-27 0.17950822124858945 1102 28-29 0.18753331790402683 1102 30-31 0.27443705310141553 1102 32-33 0.13775664038085011 1102 34-35 0.08462934910794928 1102 36-37 0.18388559278258754 1102 38-39 0.1460406608338971 1102 40-41 0.36283833426983847 1102 42-43 0.3697163033946822 1102 44-45 0.35961326361139356 1102 46-47 0.22752544356358584 1102 48-49 0.4058922913808445 1102 50-51 0.33734088722888345 1102 52-53 0.23809828228741736 1102 54-55 0.13577837102283752 1102 56-57 0.184733559762158 1102 58-59 0.17852716966297066 1102 60-61 0.25921780695258434 1102 62-63 0.0783901340548141 1102 64-65 0.2953303805907481 1102 66-67 0.24361512246846928 1102 68-69 0.3400027490101927 1102 70-71 0.24800797843618838 1102 72-73 0.14320278365775252 1102 74-75 0.19576997966969856 1102 76-77 0.2748845155371171 1102 78-79 0.16921496658084578 1102 80-81 0.5298413530946675 1102 82-83 0.33878962130022927 1102 84-85 0.41332950539532476 1102 86-87 0.049149748641536206 1102 88-89 0.15336582329067383 1102 90-91 0.09865238202280224 1102 92-93 0.2560259525519015 1102 94-95 0.2444039934295894 1102 96-97 0.1735054453695426 1102 98-99 0.3463887053997219 1102 100-101 0.45679074390906393 1103 1 0.11753952728851402 1103 2 0.043269908019127 1103 3 -0.038071316937880795 1103 4 0.004628487709425144 1103 5 0.04395520593130442 1103 6 0.08303007066362511 1103 7 -0.01578919983094096 1103 8 0.022056403178318362 1103 9 0.07068215306241399 1103 10-11 0.052833093578477985 1103 12-13 0.14383844461595174 1103 14-15 0.21237946054801426 1103 16-17 0.24986900515857968 1103 18-19 0.1420833216992179 1103 20-21 0.02390237688246799 1103 22-23 0.07937627747148923 1103 24-25 0.04932698675577285 1103 26-27 -0.07397898411840487 1103 28-29 -0.05303364614680106 1103 30-31 -0.07256016721259329 1103 32-33 -0.05948583108679628 1103 34-35 0.11740274356324676 1103 36-37 0.0312481517240073 1103 38-39 0.024674131335949312 1103 40-41 0.21939994199693302 1103 42-43 0.28071447818364703 1103 44-45 0.19094242185785504 1103 46-47 0.3219380598253778 1103 48-49 0.32678091645522755 1103 50-51 0.4694926872811962 1103 52-53 0.4410820539900513 1103 54-55 0.5280768400835427 1103 56-57 0.49102479467341453 1103 58-59 0.5507331257941743 1103 60-61 0.40407049693423147 1103 62-63 0.6020860122495861 1103 64-65 0.5598040521253864 1103 66-67 0.4985129202842913 1103 68-69 0.4772785529960082 1103 70-71 0.3800567722629289 1103 72-73 0.5497401960376216 1103 74-75 0.35846460770977373 1103 76-77 0.5416813850371227 1103 78-79 0.4947009299447167 1103 80-81 0.8342810696274618 1103 82-83 0.6606744425547859 1103 84-85 0.6007489623079714 1103 86-87 0.4943474724262664 1103 88-89 0.3886257435683014 1103 90-91 0.4375958493902097 1103 92-93 0.43143592103496076 1103 94-95 0.7273112589255426 1103 96-97 0.7560315878308579 1103 98-99 1.1143538433502513 1103 100-101 1.1884028358024743 1104 1 0.12299979878621059 1104 2 0.05656327154636642 1104 3 0.12354343847908211 1104 4 0.10877581913707246 1104 5 -0.05215485334280601 1104 6 0.015594944257564691 1104 7 0.046897191873156885 1104 8 0.09597888897852158 1104 9 0.04137611799293239 1104 10-11 0.09666994747212954 1104 12-13 0.05192490561754681 1104 14-15 0.18325440331571485 1104 16-17 0.19265216586689604 1104 18-19 0.061415157711643076 1104 20-21 0.04856486095022916 1104 22-23 0.08292004007805787 1104 24-25 0.020067356259922065 1104 26-27 0.19563932279525176 1104 28-29 -0.028938844696504873 1104 30-31 0.09138409780083379 1104 32-33 0.24174327138688056 1104 34-35 0.17033334707019776 1104 36-37 -0.005434663734284584 1104 38-39 0.31047525989518476 1104 40-41 0.35206288392315344 1104 42-43 0.08020959642249892 1104 44-45 0.20613063880852422 1104 46-47 0.2659617694688663 1104 48-49 0.331924090033084 1104 50-51 0.4179851573657203 1104 52-53 0.46522790047134777 1104 54-55 0.34054048983419705 1104 56-57 0.2968665117663889 1104 58-59 0.24620465813507053 1104 60-61 0.3706793125657626 1104 62-63 0.5211224058098622 1104 64-65 0.4404035373439825 1104 66-67 0.38402046257432687 1104 68-69 0.5212778228973853 1104 70-71 0.4510418485520482 1104 72-73 0.609727388375294 1104 74-75 0.7243476558096589 1104 76-77 0.7448019379321522 1104 78-79 1.0592574259662975 1104 80-81 1.0397388662145595 1104 82-83 1.071523522506773 1104 84-85 0.7675099849410252 1104 86-87 0.7719922237596215 1104 88-89 0.7465952650443377 1104 90-91 0.8210275604196475 1104 92-93 0.8606751335184057 1104 94-95 0.9636775951916441 1104 96-97 0.8531548136293239 1104 98-99 0.9488596655774231 1104 100-101 1.1257643954911316 1105 1 -0.015957661942358925 1105 2 0.1577061730120093 1105 3 0.04499214786712713 1105 4 0.07162393363631026 1105 5 -0.038383716916321475 1105 6 0.11763995885999634 1105 7 -0.015618405410052105 1105 8 0.029429918567828395 1105 9 0.04591029037579375 1105 10-11 0.06952224372945892 1105 12-13 0.09512600771314794 1105 14-15 0.05238645244462958 1105 16-17 -0.06131375954942797 1105 18-19 -0.033120397349293285 1105 20-21 -0.10530360252346327 1105 22-23 -0.1613511199551212 1105 24-25 0.12934951027002484 1105 26-27 0.1861182352683599 1105 28-29 0.058276947510584876 1105 30-31 0.047843227860248305 1105 32-33 0.1822636642023454 1105 34-35 0.22623148079203048 1105 36-37 0.05293037002329726 1105 38-39 0.23124864907521925 1105 40-41 0.12395388167714572 1105 42-43 0.1903989221477289 1105 44-45 0.21446801900452783 1105 46-47 0.4261433387073126 1105 48-49 0.5483359729708326 1105 50-51 0.2902619560275568 1105 52-53 0.263038210528471 1105 54-55 0.34325526020846553 1105 56-57 0.5714436146174009 1105 58-59 0.4261973063345579 1105 60-61 0.34474145214205976 1105 62-63 0.43280805900722896 1105 64-65 0.5526053341510107 1105 66-67 0.33316657455101506 1105 68-69 0.42450974859698576 1105 70-71 0.30854928466220244 1105 72-73 0.3856854831123506 1105 74-75 0.3512985808550191 1105 76-77 0.22940446857027297 1105 78-79 0.32497864434175483 1105 80-81 0.6339245362738488 1105 82-83 0.7155167979699684 1105 84-85 0.49054186072931927 1105 86-87 0.5347870545670403 1105 88-89 0.6179092476293384 1105 90-91 0.5308776241577355 1105 92-93 0.7514486238700115 1105 94-95 0.7855448176274535 1105 96-97 0.915647647310017 1105 98-99 1.394704212092737 1105 100-101 1.3053329662066844 1106 1 -0.03243286171688453 1106 2 -0.060871421968968775 1106 3 -0.08996721870019542 1106 4 -0.07368299622991259 1106 5 0.07923663059478514 1106 6 0.03139112671234301 1106 7 0.049781326310124996 1106 8 -0.04720729299221205 1106 9 0.007004496262290161 1106 10-11 -0.018392898422312953 1106 12-13 0.0655852150871894 1106 14-15 0.19884688117040383 1106 16-17 0.09391795801560221 1106 18-19 -0.07913899947570258 1106 20-21 0.03464034056363374 1106 22-23 0.16166901147067847 1106 24-25 -0.12657978703517614 1106 26-27 0.007648213190080355 1106 28-29 0.07307278865135203 1106 30-31 -0.06560155816831781 1106 32-33 -0.027979771032086376 1106 34-35 -0.06068178757652731 1106 36-37 0.04886572189264626 1106 38-39 0.11379140943453336 1106 40-41 0.25064824623498083 1106 42-43 0.1336165054371392 1106 44-45 0.3511380609167247 1106 46-47 0.23881460197124937 1106 48-49 0.5987428292427879 1106 50-51 0.4249655694540735 1106 52-53 0.3840485920182104 1106 54-55 0.42139306417629285 1106 56-57 0.32078465882093354 1106 58-59 0.23184449226708637 1106 60-61 0.18669775489114215 1106 62-63 0.03676127184081679 1106 64-65 0.44358011897910643 1106 66-67 0.2060350809004774 1106 68-69 0.1076263876875494 1106 70-71 0.45732845487374973 1106 72-73 0.3269027114790255 1106 74-75 0.18354017884239582 1106 76-77 0.11164972017395058 1106 78-79 0.22085971391182824 1106 80-81 0.6992577199828958 1106 82-83 0.3956296425190331 1106 84-85 0.1381858001628018 1106 86-87 0.6231184480040994 1106 88-89 0.48189424692471405 1106 90-91 0.5594345501093692 1106 92-93 0.5913171458778308 1106 94-95 0.5148901417815104 1106 96-97 0.9961211559821344 1106 98-99 0.7150350209511913 1106 100-101 0.7271507968981012 1107 1 -0.047579178914730846 1107 2 -0.08661844588059608 1107 3 -0.12073328271611672 1107 4 -0.010830068170633922 1107 5 -0.06840479792763432 1107 6 -8.123794113643612E-4 1107 7 -0.1407363586876471 1107 8 -0.03692220602524543 1107 9 -0.16254505946860576 1107 10-11 -0.3781050669358166 1107 12-13 -0.2445163321356958 1107 14-15 -0.09280291813660568 1107 16-17 -0.020720623067802535 1107 18-19 -0.014280856829302024 1107 20-21 -0.25260107423618905 1107 22-23 -0.2417873678219209 1107 24-25 -0.022722021760202438 1107 26-27 -0.17149172299983917 1107 28-29 -0.05089544353500486 1107 30-31 -0.19145493221202514 1107 32-33 -0.11114385899393397 1107 34-35 -0.03247841867879231 1107 36-37 0.025864932843276733 1107 38-39 0.12883652247442967 1107 40-41 0.1432551967307134 1107 42-43 -0.16609532001142213 1107 44-45 -0.13501069204220784 1107 46-47 -0.08713483025627511 1107 48-49 0.2915075896119248 1107 50-51 0.3268970250786225 1107 52-53 0.2838496143517446 1107 54-55 0.335105369915766 1107 56-57 0.3351728024965368 1107 58-59 0.390290751143894 1107 60-61 0.43910264013592126 1107 62-63 0.736414776249191 1107 64-65 0.27077659227149553 1107 66-67 0.34501926560664486 1107 68-69 0.2219527796735541 1107 70-71 0.2725107938905218 1107 72-73 0.4245132641566478 1107 74-75 0.327401031701843 1107 76-77 0.23955633789668695 1107 78-79 0.5575007650628088 1107 80-81 0.8122684579155859 1107 82-83 0.5209992949942475 1107 84-85 0.5815200483359817 1107 86-87 0.4041263728042672 1107 88-89 0.23007600273108864 1107 90-91 0.459889111157775 1107 92-93 0.514935451823149 1107 94-95 0.7195626862941857 1107 96-97 0.35524839876919145 1107 98-99 0.44057620589750357 1107 100-101 0.44524061129996895 1108 1 -0.12339042705607284 1108 2 -0.20747473906698133 1108 3 -0.3051249559427376 1108 4 -0.029783973532303776 1108 5 -0.20345684026123223 1108 6 -0.06419032045920403 1108 7 -0.24774676715436783 1108 8 -9.605161800649853E-4 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-0.3256502275469586 2316 80-81 0.04769991712439747 2316 82-83 -0.2971212672431065 2316 84-85 -0.724558780819212 2316 86-87 -0.40292514146903 2316 88-89 -0.2488853796772581 2316 90-91 -0.32200300592670317 2316 92-93 -0.3541795315125995 2316 94-95 -0.4773109552070487 2316 96-97 -0.596667990159947 2316 98-99 -1.2619143751777067 2316 100-101 -1.409738739518751 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 2.0 5 14.0 6 25.0 7 87.0 8 170.0 9 364.0 10 595.0 11 1112.0 12 1695.0 13 2562.0 14 3129.0 15 4562.0 16 6394.0 17 9555.0 18 8332.0 19 4430.0 20 3493.0 21 3537.0 22 3487.0 23 3687.0 24 4103.0 25 4772.0 26 5652.0 27 6966.0 28 8623.0 29 10567.0 30 12867.0 31 16211.0 32 20552.0 33 27535.0 34 38317.0 35 55225.0 36 85404.0 37 128336.0 38 96554.0 39 7683.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.468108325631675 19.891585699816183 15.403936199599627 20.23636977495251 2 15.678996980644413 22.02271250995228 40.04439526791453 22.253895241488774 3 19.359969993265533 27.47849659440613 30.71598455335146 22.44554885897687 4 12.742540939171704 17.89987078121111 37.81763444379664 31.53995383582054 5 12.851653465987937 39.07508873379181 33.29281639861845 14.78044140160181 6 29.416878905970318 38.007882687068914 17.563697362095596 15.011541044865172 7 26.80573881439204 35.30042516050065 20.68301846908125 17.21081755602606 8 26.97297315726847 33.88055274274551 19.602387990494407 19.544086109491616 9 27.975404065114105 16.678969180072997 20.53968540796896 34.80594134684394 10-11 24.64285836052383 27.81638998844191 26.65282423176191 20.88792741927235 12-13 26.81008588446681 25.70712339882919 27.559486670689836 19.923304046014163 14-15 23.486793430639512 25.934023409398538 27.684699336176827 22.894483823785123 16-17 20.864657808872114 29.539790181417725 27.888074026341542 21.707477983368623 18-19 20.883635808799024 26.94141343882682 32.17943439602507 19.995516356349086 20-21 22.28066676213271 25.696816889307122 32.144695838362345 19.877820510197818 22-23 21.670740979403412 26.105775295686 32.10558436554938 20.117899359361203 24-25 21.57790520157898 26.15203513919208 32.325601813217766 19.94445784601117 26-27 21.49026126241316 26.222222222222225 32.30209265652304 19.985423858841582 28-29 20.887514800011253 26.518892443448827 32.27762050767981 20.315972248860113 30-31 21.086676283723644 26.35551053954418 32.19574121249945 20.362071964232733 32-33 20.98764064098193 26.503068530514835 32.0827650869417 20.42652574156154 34-35 21.350303834131882 26.268845259821017 32.098590744928494 20.282260161118607 36-37 21.63112314791372 26.167684935839237 31.89701543557966 20.304176480667376 38-39 21.385792755901218 26.42283838015323 31.941494167828782 20.249874696116773 40-41 21.26123192513556 26.531339425985866 31.82906589083519 20.378362758043384 42-43 21.203728566496604 26.865029132089624 31.273500667033215 20.657741634380557 44-45 21.54008762508737 26.97940639969996 30.794592475153003 20.685913500059666 46-47 21.89416798786205 27.240747370394995 29.996590463526484 20.86849417821647 48-49 22.07481697228821 27.4838622789454 29.371973511378872 21.069347237387518 50-51 22.02838809104919 27.803520041460388 29.17732157713132 20.990770290359094 52-53 22.156569087659154 28.046342854269078 28.93445019430278 20.862637863768988 54-55 21.660451339314253 27.869669912843353 29.028576299358576 21.441302448483814 56-57 22.001467781680688 28.311820927225472 28.56625346595079 21.12045782514305 58-59 22.199814353745616 27.66930418454158 28.891517977768476 21.239363483944327 60-61 22.052601252638073 27.534938510267608 29.346984838101477 21.065475398992845 62-63 21.986817285670085 27.51441992562242 29.60135628701938 20.897406501688113 64-65 21.844538950505363 27.700391350564352 29.329555364042974 21.125514334887313 66-67 21.7836685987044 27.849897715649508 28.96743948175929 21.398994203886808 68-69 21.744011210441258 27.92586379723012 29.048101248559487 21.282023743769134 70-71 21.94262480234922 27.901513440252995 28.825773235420726 21.33008852197706 72-73 21.870559161032546 27.811341439191995 28.59084054452411 21.727258855251346 74-75 21.868963859447536 28.302711862673064 28.28370308112743 21.54462119675197 76-77 21.90762269992252 28.169902494700246 28.389138119295886 21.53333668608135 78-79 21.861304503865533 27.930479200108433 28.343331409588096 21.864884886437938 80-81 22.035894865684057 28.352102447656165 28.132703263882497 21.479299422777284 82-83 21.882498553032324 28.323145601175646 28.22060055764728 21.573755288144753 84-85 21.79970081699973 28.09967652436296 27.994323194353882 22.10629946428343 86-87 21.849908455818426 27.85193367905326 28.106876553438276 22.19128131169004 88-89 21.97913381464213 27.644371329452177 28.036976107876814 22.339518748028876 90-91 21.736154558209073 27.76623292493535 28.35010509699097 22.14750741986461 92-93 21.742528786578283 27.95057632822282 28.11440134401293 22.192493541185968 94-95 21.507915130975558 28.283253608092767 27.771489162936113 22.43734209799556 96-97 21.678078484553772 28.19223337217301 27.63290797213431 22.496780171138912 98-99 21.621054910547997 28.33705108851951 27.233913428156693 22.807980572775804 100-101 21.47290720173203 28.44338939131769 27.2344633009146 22.84924010603568 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 595.0 1 423.5 2 259.5 3 290.5 4 256.5 5 263.5 6 490.0 7 994.5 8 1613.5 9 1910.0 10 1675.0 11 1348.0 12 1340.0 13 1458.0 14 1577.0 15 1658.0 16 1641.5 17 1686.5 18 1807.5 19 1868.0 20 1954.5 21 2123.0 22 2357.5 23 2697.5 24 3104.0 25 3656.0 26 4445.5 27 5225.5 28 5928.5 29 6623.5 30 7552.0 31 8826.0 32 10170.5 33 11386.5 34 12545.5 35 13403.5 36 14668.5 37 16008.5 38 16578.0 39 16780.0 40 16878.5 41 17548.0 42 18017.5 43 18428.0 44 19550.0 45 20499.5 46 20587.0 47 21119.5 48 24767.0 49 24391.5 50 20502.5 51 19207.5 52 17900.0 53 17408.0 54 16988.5 55 15692.0 56 14249.0 57 12664.5 58 11528.0 59 10794.0 60 9520.5 61 7667.5 62 6046.5 63 4627.0 64 3244.0 65 2370.5 66 1676.5 67 1265.0 68 904.5 69 507.0 70 336.0 71 225.5 72 171.5 73 141.0 74 95.5 75 73.5 76 54.0 77 27.5 78 12.5 79 7.5 80 5.5 81 2.5 82 1.0 83 1.5 84 1.0 85 0.5 86 0.0 87 1.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025230053767290258 2 0.00903508682207016 3 0.011421713529786806 4 0.0 5 0.0027275733802475956 6 0.0 7 0.0 8 0.0 9 1.7047333626547473E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.004347070074769605 20-21 0.0010228400175928483 22-23 0.0 24-25 0.003494703393442232 26-27 0.004602780079167817 28-29 0.005369910092362453 30-31 0.024548160422228363 32-33 3.4094667253094945E-4 34-35 0.002642336712114858 36-37 0.011506950197919544 38-39 0.004773253415433293 40-41 0.008267956808875524 42-43 0.008438430145140998 44-45 0.0020456800351856966 46-47 0.0020456800351856966 48-49 0.0017899700307874844 50-51 0.0030685200527785454 52-53 0.0029832833846458076 54-55 0.0024718633758493832 56-57 4.261833406636868E-4 58-59 4.261833406636868E-4 60-61 0.0 62-63 8.523666813273736E-5 64-65 0.0074155901275481505 66-67 3.4094667253094945E-4 68-69 0.0010228400175928483 70-71 0.0041765967385041305 72-73 0.012188843542981442 74-75 0.005284673424229716 76-77 0.0031537567209112822 78-79 0.012359316879246917 80-81 0.0 82-83 0.005199436756096979 84-85 7.671300131946363E-4 86-87 0.0 88-89 0.0011933133538583232 90-91 1.7047333626547473E-4 92-93 8.523666813273736E-5 94-95 6.818933450618989E-4 96-97 4.261833406636868E-4 98-99 4.261833406636868E-4 100-101 0.0011933133538583232 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 586602.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.42636008407616 #Duplication Level Percentage of deduplicated Percentage of total 1 81.13650897345438 49.02783907194831 2 11.020427720144765 13.31848667396004 3 3.3607634276578535 6.092361031111423 4 1.4050667588654024 3.3961227965346645 5 0.7647735075752014 2.310623967575053 6 0.4705069330010649 1.7058612813345986 7 0.30107804008983097 1.273513504471323 8 0.22579295826528775 1.0915077280469645 9 0.1853901925305559 1.008220907691682 >10 1.0347529959576047 11.611584895464034 >50 0.062259375898303174 2.5927897652725496 >100 0.029861951339040963 3.2812032967741303 >500 0.00112686608826522 0.4914570125958303 >1k 0.0014085826103315251 1.8255576733902612 >5k 2.81716522066305E-4 0.9728703938292935 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5715 0.974255116757188 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4296 0.7323534525964794 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3110 0.5301720757856264 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1197 0.20405658350977326 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1098 0.18717972321949122 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1023 0.17439422299958063 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 785 0.13382156896839767 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 759 0.12938926222549532 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 698 0.11899038871330135 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 645 0.10995530189123119 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.7047333626547473E-4 0.0 16-17 0.0 0.0 0.0 3.4094667253094945E-4 0.0 18-19 0.0 0.0 0.0 3.4094667253094945E-4 0.0 20-21 0.0 0.0 0.0 5.114200087964242E-4 0.0 22-23 0.0 0.0 0.0 7.671300131946362E-4 0.0 24-25 0.0 0.0 0.0 0.0017047333626547473 0.0 26-27 0.0 0.0 0.0 0.003324230057176757 0.0 28-29 8.523666813273736E-5 0.0 0.0 0.005114200087964241 0.0 30-31 1.7047333626547473E-4 0.0 0.0 0.008353193477008261 0.0 32-33 1.7047333626547473E-4 0.0 0.0 0.012018370206715968 0.0 34-35 1.7047333626547473E-4 0.0 0.0 0.014916416923229037 0.0 36-37 1.7047333626547473E-4 0.0 0.0 0.01772922697160937 0.0 38-39 5.114200087964242E-4 0.0 0.0 0.0219058237101135 0.0 40-41 5.114200087964242E-4 0.0 0.0 0.02633813045301584 0.0 42-43 5.966566769291615E-4 0.0 0.0 0.03852697399599729 0.0 44-45 6.818933450618989E-4 0.0 0.0 0.05327291758296085 0.0 46-47 6.818933450618989E-4 0.0 0.0 0.07176927456776486 0.0 48-49 8.523666813273736E-4 0.0 0.0 0.11379095195720437 0.0 50-51 8.523666813273736E-4 0.0 0.0 0.15163603260813976 0.0 52-53 8.523666813273736E-4 0.0 0.0 0.19348723666131382 0.0 54-55 8.523666813273736E-4 0.0 0.0 0.2771896447676619 0.0 56-57 8.523666813273736E-4 0.0 0.0 0.3586758995025588 0.0 58-59 8.523666813273736E-4 0.0 0.0 0.4503053177452515 0.0 60-61 8.523666813273736E-4 0.0 0.0 0.5589820696144916 0.0 62-63 8.523666813273736E-4 0.0 0.0 0.7084871855193129 0.0 64-65 8.523666813273736E-4 0.0 0.0 0.7947466936696432 0.0 66-67 8.523666813273736E-4 0.0 0.0 0.9433994428931372 0.0 68-69 8.523666813273736E-4 0.0 0.0 1.1546159065260602 0.0 70-71 8.523666813273736E-4 0.0 0.0 1.2751405552657509 0.0 72-73 8.523666813273736E-4 0.0 0.0 1.4780890620897986 0.0 74-75 8.523666813273736E-4 0.0 0.0 1.7408737099430278 0.0 76-77 8.523666813273736E-4 0.0 0.0 1.9087899461645206 0.0 78-79 8.523666813273736E-4 0.0 0.0 2.058209825401209 0.0 80-81 8.523666813273736E-4 0.0 0.0 2.307441843021333 0.0 82-83 8.523666813273736E-4 0.0 0.0 2.6342392286422482 0.0 84-85 9.37603349460111E-4 0.0 0.0 3.0274359787385654 0.0 86-87 0.0010228400175928483 0.0 0.0 3.601078755271888 0.0 88-89 0.0010228400175928483 0.0 0.0 4.193217888790015 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTATAC 110 7.0043825E-8 34.544495 3 CCCTATA 115 1.0344047E-7 33.045376 2 GGTATCA 4450 0.0 29.047758 1 GTATCAA 6395 0.0 28.610397 1 GTACACG 110 9.437771E-5 25.921629 1 CTTAGAC 95 0.0012260369 24.999306 3 GTGTAGG 210 1.2551027E-9 24.892994 1 AGTACTT 4280 0.0 23.467894 12-13 GAGTACT 4190 0.0 23.291918 12-13 TCAACGC 7955 0.0 23.043829 4 TATCAAC 7920 0.0 23.031624 2 ATCAACG 7920 0.0 23.029661 3 CAACGCA 8075 0.0 22.70138 5 GTACATG 4200 0.0 22.516846 1 GTACTTT 4385 0.0 22.256134 14-15 AACGCAG 8295 0.0 22.099295 6 GCGGTAT 110 0.002867641 21.601358 1 ACATGGG 4155 0.0 21.49158 3 TACATGG 4270 0.0 21.35954 2 TACTTTT 4715 0.0 21.353134 14-15 >>END_MODULE