FastQCFastQC Report
Thu 26 May 2016
SRR936989_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936989_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654741
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57450.8774461962821941No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40620.6203979894339899No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28510.4354393569365596No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9000.13745893414342467No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA8090.12356030858003393No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT6900.10538518284329222No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6750.10309420060756848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38800.034.7766531
TATACCG300.003944886431.67693180-81
GTATCAA57900.031.5103971
GTATAGG1303.0269257E-729.2380121
GTACATG42950.027.2127931
CGTAACA700.00836635527.1578852
ACGTAAC700.00837884327.1495841
TACATGG44000.026.2474772
ATCAACG69550.026.103593
TATCAAC69200.026.0982552
TCAACGC70300.025.8251024
CAACGCA71200.025.4986615
AACGCAG72750.024.9572966
ACATGGG43850.024.6031233
CATGGGT3300.024.4832464
GAGTACT41050.023.96117812-13
GTATATG2202.1591404E-923.7558861
GTCTATA1652.4220044E-623.0360091
CCGGGTC1252.249518E-422.8073929
CATGGGA32700.022.3824164
Produced by FastQC (version 0.11.5)