FastQCFastQC Report
Thu 26 May 2016
SRR936989_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936989_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654741
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61100.9331934306848051No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47510.7256304401282339No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32460.4957685558106183No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12300.18786054332934704No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11300.17258732842452207No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10950.16724170320783333No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8290.12661495156099892No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8240.1258512908157577No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA7770.11867287981048993No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6830.1043160577999545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52750.028.8195461
GTATCAA75750.028.3475421
TCGCGAT350.0083904627.14387150-51
TCAATCG350.00839827927.13868914-15
AGTACTT48650.023.47789612-13
GAGTACT46850.023.41689512-13
TATCAAC91400.023.3306622
ATCAACG91500.023.2532623
TCAACGC92000.023.1268844
CAACGCA92300.023.0569975
AACGCAG94850.022.4872026
GTACATG46500.021.9657021
GTACTTT50500.021.72438814-15
TACTTTT53800.021.14219914-15
TACATGG48500.020.7596992
ACGCAGA103850.020.4926417
ATTACGG704.8497616E-420.3586880-81
CGCAGAG103700.020.3390488
ACATGGG48450.019.8989053
TTGCGAT600.00476386819.7916544-45
Produced by FastQC (version 0.11.5)