##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936989_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 654741 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22271249242067 33.0 31.0 34.0 31.0 34.0 2 32.33104693306208 33.0 31.0 34.0 31.0 34.0 3 32.41761398782114 34.0 31.0 34.0 31.0 34.0 4 36.08016757771394 37.0 35.0 37.0 35.0 37.0 5 35.999919051961 37.0 35.0 37.0 35.0 37.0 6 36.05635052639135 37.0 35.0 37.0 35.0 37.0 7 36.0051027810997 37.0 35.0 37.0 35.0 37.0 8 35.98027617027191 37.0 35.0 37.0 35.0 37.0 9 37.645485772236654 39.0 37.0 39.0 35.0 39.0 10-11 37.57337557904576 39.0 37.0 39.0 35.0 39.0 12-13 37.47572169758729 39.0 37.0 39.0 35.0 39.0 14-15 38.628774584148545 40.0 38.0 41.0 34.0 41.0 16-17 38.55949451767951 40.0 38.0 41.0 34.0 41.0 18-19 38.76101236977675 40.0 38.0 41.0 35.0 41.0 20-21 38.80597824177805 40.0 38.0 41.0 35.0 41.0 22-23 38.71706445754886 40.0 38.0 41.0 34.5 41.0 24-25 38.59230825624178 40.0 38.0 41.0 34.0 41.0 26-27 38.392713301901054 40.0 38.0 41.0 34.0 41.0 28-29 38.2939742585236 40.0 38.0 41.0 34.0 41.0 30-31 38.23030328022837 40.0 37.5 41.0 34.0 41.0 32-33 38.00103017834533 40.0 37.0 41.0 33.5 41.0 34-35 37.81036318177722 40.0 37.0 41.0 33.0 41.0 36-37 37.62654774941542 40.0 36.5 41.0 33.0 41.0 38-39 37.43125602337413 40.0 36.0 41.0 33.0 41.0 40-41 37.213751697236006 40.0 36.0 41.0 32.5 41.0 42-43 36.90369397975688 40.0 36.0 41.0 31.5 41.0 44-45 36.505901570239224 39.0 35.0 41.0 30.5 41.0 46-47 35.96459057856465 39.0 35.0 41.0 29.5 41.0 48-49 35.45572753195539 39.0 35.0 40.5 27.0 41.0 50-51 35.09931484357937 39.0 35.0 40.0 26.0 41.0 52-53 34.83001980935973 38.5 35.0 40.0 24.5 41.0 54-55 34.518082722786566 38.0 34.0 40.0 23.5 41.0 56-57 34.22475146661046 38.0 34.0 40.0 23.0 41.0 58-59 33.879702813784384 38.0 34.0 40.0 22.0 41.0 60-61 33.53477176471307 37.0 33.0 40.0 21.5 41.0 62-63 33.172438414579204 37.0 33.0 39.5 20.0 41.0 64-65 32.772577248102685 36.0 32.5 39.0 18.5 40.0 66-67 32.38740280507865 36.0 32.0 39.0 18.0 40.0 68-69 32.077253448310096 35.0 31.5 38.5 14.5 40.0 70-71 31.61354413424545 35.0 31.0 38.0 10.0 40.0 72-73 31.09739194582285 35.0 31.0 37.0 7.5 39.0 74-75 30.605136229440344 34.5 30.0 36.5 7.0 39.0 76-77 29.187611589926398 33.0 28.5 35.0 2.0 37.5 78-79 29.892214631434413 34.0 30.0 36.0 2.0 38.0 80-81 29.60206402226224 34.0 30.0 35.0 2.0 37.0 82-83 29.322848271301172 34.0 29.0 35.0 2.0 37.0 84-85 29.00422380758193 34.0 29.0 35.0 2.0 36.0 86-87 28.68586204315905 34.0 29.0 35.0 2.0 36.0 88-89 28.30002626992964 34.0 29.0 35.0 2.0 35.5 90-91 27.87694294385108 33.5 27.5 35.0 2.0 35.0 92-93 27.40811404815034 33.0 27.0 35.0 2.0 35.0 94-95 26.83165557067604 33.0 25.5 35.0 2.0 35.0 96-97 26.155175099772276 32.5 25.0 34.0 2.0 35.0 98-99 25.3907002921766 32.0 22.0 34.0 2.0 35.0 100-101 22.129436678014667 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06041592833769727 1101 2 -0.029477172047741362 1101 3 -0.011920997396202893 1101 4 0.15299296998188794 1101 5 0.04017788292377844 1101 6 0.05496363689196926 1101 7 0.07432592874358335 1101 8 0.16218269404854624 1101 9 0.013633972129213134 1101 10-11 0.07009556779641457 1101 12-13 0.0844645500598844 1101 14-15 0.17457990329246798 1101 16-17 0.10115789012815668 1101 18-19 0.06896789950417315 1101 20-21 0.08243208062759066 1101 22-23 0.16799030657196568 1101 24-25 0.17870195606278827 1101 26-27 0.12982106800645 1101 28-29 0.1658204590423651 1101 30-31 0.22449978212411992 1101 32-33 0.243804457752141 1101 34-35 0.35636999191808627 1101 36-37 0.30315830252580866 1101 38-39 0.30230448428077494 1101 40-41 0.3284094538287263 1101 42-43 0.38511917647419125 1101 44-45 0.5794814602384193 1101 46-47 0.5594437430241896 1101 48-49 0.7091554543182852 1101 50-51 0.6545599021352757 1101 52-53 0.6830287250167117 1101 54-55 0.7102842553725282 1101 56-57 0.8738535519805097 1101 58-59 0.8194112498936974 1101 60-61 0.8274406730891926 1101 62-63 0.8252928829298796 1101 64-65 0.8847884721167034 1101 66-67 0.6827837693450363 1101 68-69 0.6098242049219209 1101 70-71 0.6863134199314622 1101 72-73 0.7249404969105733 1101 74-75 0.8146701956953635 1101 76-77 0.5941918602931473 1101 78-79 0.7425463727974808 1101 80-81 1.0479969458771983 1101 82-83 1.090142341475694 1101 84-85 0.8155181709099004 1101 86-87 0.4797315611137343 1101 88-89 0.45202159862352786 1101 90-91 0.6931512386744423 1101 92-93 1.0167677236368 1101 94-95 0.7749119067214494 1101 96-97 0.8673282339958526 1101 98-99 0.8892388185213065 1101 100-101 0.7203289776656625 1102 1 0.18739122039302458 1102 2 0.15977659940057265 1102 3 0.15808847121024883 1102 4 0.09238429213202437 1102 5 0.12251957166253646 1102 6 0.006449824242423574 1102 7 0.049346081520830865 1102 8 0.030485894181616402 1102 9 0.1900059604086195 1102 10-11 0.13361680178615387 1102 12-13 0.17246820954832032 1102 14-15 0.2370535127508404 1102 16-17 0.2883531993293431 1102 18-19 0.16088695571443168 1102 20-21 0.07016447733661124 1102 22-23 0.1362816709469712 1102 24-25 0.09372121316104653 1102 26-27 0.025589624555777846 1102 28-29 0.09172861355978768 1102 30-31 0.083862186638342 1102 32-33 0.1273373995458016 1102 34-35 0.0764607114042235 1102 36-37 -0.10140710123348384 1102 38-39 0.10248649904668383 1102 40-41 -0.0627223645275734 1102 42-43 0.010304709978981919 1102 44-45 -0.06003173823125252 1102 46-47 -0.2114431711057847 1102 48-49 -0.09812641585944704 1102 50-51 -0.006487978304370756 1102 52-53 -0.022585582815359828 1102 54-55 0.2822040793076255 1102 56-57 0.1954183031736747 1102 58-59 0.19993247108802592 1102 60-61 0.34906172410450864 1102 62-63 0.08034067380162924 1102 64-65 0.2558618794075258 1102 66-67 0.18806507652454485 1102 68-69 0.11648101911971054 1102 70-71 0.062484200323769556 1102 72-73 0.2522363140896964 1102 74-75 0.06015661336327227 1102 76-77 -0.12000017328201551 1102 78-79 -0.17860188766596607 1102 80-81 0.14961492599312365 1102 82-83 0.00795899598671923 1102 84-85 -0.05924031564851617 1102 86-87 -0.07053682269750894 1102 88-89 -0.029855296697189004 1102 90-91 -0.0071881596851817164 1102 92-93 0.12217154690653942 1102 94-95 0.05319776906838669 1102 96-97 0.14304061867718332 1102 98-99 0.017573623327265864 1102 100-101 -0.015658444442255615 1103 1 0.22657797987852035 1103 2 0.19588604571471535 1103 3 0.18281838991693178 1103 4 0.13807528532429103 1103 5 0.11918869710626723 1103 6 0.14129837894252972 1103 7 -0.09648629135271136 1103 8 0.06441701768906682 1103 9 0.22787112722127034 1103 10-11 0.10634550647166208 1103 12-13 0.11504808138456468 1103 14-15 0.2948885595444466 1103 16-17 0.3110988081071753 1103 18-19 0.18776446251448675 1103 20-21 0.12642821191162312 1103 22-23 0.1475709153340432 1103 24-25 0.11506262972397963 1103 26-27 0.30855644643275326 1103 28-29 0.2731633099776616 1103 30-31 0.26928087027057046 1103 32-33 0.2531345811725956 1103 34-35 0.3552591377869021 1103 36-37 0.25745909519206833 1103 38-39 0.2930129078491035 1103 40-41 0.3648608881271329 1103 42-43 0.33499582611009515 1103 44-45 0.3529986276655066 1103 46-47 0.2994810985605909 1103 48-49 0.590108335880906 1103 50-51 0.5132571540854087 1103 52-53 0.575317925552163 1103 54-55 0.526103238713219 1103 56-57 0.6666775043784767 1103 58-59 0.6898495641988376 1103 60-61 0.656654735030699 1103 62-63 0.6970365384459498 1103 64-65 0.5359434800714027 1103 66-67 0.49313784095833313 1103 68-69 0.3812720999059067 1103 70-71 0.38754797485390924 1103 72-73 0.4794119476089769 1103 74-75 0.6506895532922741 1103 76-77 0.40949746782873575 1103 78-79 0.7990001900006511 1103 80-81 0.9634931857936593 1103 82-83 0.898576072193439 1103 84-85 0.7469465821431953 1103 86-87 0.47496399945607237 1103 88-89 0.49282884265400284 1103 90-91 0.5356272168918927 1103 92-93 0.5977969983226394 1103 94-95 0.5388273696707877 1103 96-97 0.4680410028610993 1103 98-99 0.327759875689992 1103 100-101 0.26508203163845323 1104 1 0.16709305525540685 1104 2 0.1378824862507244 1104 3 0.2432088840542903 1104 4 0.13970845115343167 1104 5 0.038765749032570795 1104 6 0.010864961857102173 1104 7 0.04390839038933336 1104 8 0.1902336006036407 1104 9 0.015512458879562985 1104 10-11 0.1746479562204044 1104 12-13 0.11929064838624726 1104 14-15 0.04271100510557346 1104 16-17 0.27649348984921573 1104 18-19 0.2477466651945548 1104 20-21 0.20782216082285032 1104 22-23 0.13780594464727614 1104 24-25 0.18124510459835363 1104 26-27 0.13123756033560596 1104 28-29 -0.0327935576940348 1104 30-31 0.2261930352621917 1104 32-33 0.050512093735413544 1104 34-35 0.11397982455406463 1104 36-37 0.17634519234449897 1104 38-39 0.23498841036167306 1104 40-41 0.2053969015274646 1104 42-43 0.2178161778688903 1104 44-45 0.03800438562195296 1104 46-47 0.006403732563939002 1104 48-49 0.27901903521088656 1104 50-51 0.47733274035006446 1104 52-53 0.27461911901949776 1104 54-55 0.2817931465859189 1104 56-57 0.3696585554672609 1104 58-59 0.32960563622564365 1104 60-61 0.5771007149301965 1104 62-63 0.6102431967374073 1104 64-65 0.5546673075420827 1104 66-67 0.4892930964022142 1104 68-69 0.3109334273765896 1104 70-71 0.2075673425256035 1104 72-73 0.5480075296860285 1104 74-75 0.2737030583173983 1104 76-77 0.4521089628036066 1104 78-79 0.7033552988783747 1104 80-81 0.9871531522928194 1104 82-83 0.8166220311549139 1104 84-85 0.8337451583576012 1104 86-87 0.8550126803605984 1104 88-89 0.739109726238592 1104 90-91 0.6059999137093115 1104 92-93 0.37718874874139985 1104 94-95 0.4093283118818434 1104 96-97 0.394407208891252 1104 98-99 0.7825965591070805 1104 100-101 1.262786700817685 1105 1 0.08907897936801845 1105 2 0.13265885303979275 1105 3 0.07165773146351029 1105 4 0.07241958946432447 1105 5 0.07866272985672396 1105 6 0.08266473745889868 1105 7 -0.03218682584893884 1105 8 0.11737048350857293 1105 9 0.06901995581959142 1105 10-11 0.0583746239070706 1105 12-13 0.10628383994070845 1105 14-15 0.06388603570533746 1105 16-17 0.04502291661381008 1105 18-19 -0.005360484948241151 1105 20-21 -0.04299192016450348 1105 22-23 0.016613701685223248 1105 24-25 -0.013114978020979606 1105 26-27 0.0703504224327034 1105 28-29 -0.05264514888130378 1105 30-31 -0.06531489517436029 1105 32-33 -0.23602018900633226 1105 34-35 0.01881713585557776 1105 36-37 0.15881153261377534 1105 38-39 -0.08427541052775922 1105 40-41 -0.13024161336441153 1105 42-43 0.1803799273588922 1105 44-45 -0.1779030246187645 1105 46-47 -0.14521996694647044 1105 48-49 -0.012212222439053733 1105 50-51 -0.08864925679395697 1105 52-53 -0.20527875429751674 1105 54-55 -0.09779809168417586 1105 56-57 -0.006721620991335442 1105 58-59 -0.11082301534514727 1105 60-61 -0.15878004802370072 1105 62-63 0.1274848838039091 1105 64-65 0.005566175351141567 1105 66-67 0.037708921575621446 1105 68-69 -0.04833001101589929 1105 70-71 -0.35426902528345394 1105 72-73 -0.4190511963441672 1105 74-75 -0.12255293413466006 1105 76-77 -0.21067089745750778 1105 78-79 -0.20186705934276006 1105 80-81 -0.0807273046635224 1105 82-83 0.10484558786646048 1105 84-85 0.28118242885943445 1105 86-87 0.20993987844301287 1105 88-89 0.05518161016351897 1105 90-91 -0.05231811974642042 1105 92-93 0.18162443825588426 1105 94-95 0.05122001424822287 1105 96-97 0.06171264544775923 1105 98-99 -0.19572351433233592 1105 100-101 0.226839000899286 1106 1 -0.014753493936957796 1106 2 0.016796766720624134 1106 3 -0.061586453684334685 1106 4 -0.017670403472116902 1106 5 0.10802052142610563 1106 6 0.05498206905240721 1106 7 -8.465655578078213E-4 1106 8 -0.06815479880899034 1106 9 -0.10638089648020355 1106 10-11 -0.06108304598223668 1106 12-13 -0.05194879728188795 1106 14-15 -0.06463113880043636 1106 16-17 -0.06352578327853564 1106 18-19 -0.1680343553634458 1106 20-21 -0.023838540351896143 1106 22-23 -0.03826096063906448 1106 24-25 -0.04923167587153898 1106 26-27 -0.16311357769670565 1106 28-29 -0.19133797766466643 1106 30-31 -0.13141755578038783 1106 32-33 0.01332872369135174 1106 34-35 0.11114392147036511 1106 36-37 -0.07288388254536926 1106 38-39 0.09725437952466365 1106 40-41 0.14570527157151503 1106 42-43 0.11712785187770436 1106 44-45 -0.017637593085829906 1106 46-47 0.13141979350372424 1106 48-49 0.32902132963085506 1106 50-51 0.24118644813273704 1106 52-53 0.2643928892103986 1106 54-55 -0.08720006193096452 1106 56-57 0.1998370613409719 1106 58-59 0.2003517816000837 1106 60-61 0.2763206121401822 1106 62-63 0.5246750983526809 1106 64-65 0.160114260552362 1106 66-67 0.23419260080750348 1106 68-69 0.053901030166606745 1106 70-71 0.01761460757699851 1106 72-73 0.19403129987692225 1106 74-75 0.3858244790222436 1106 76-77 0.21584565516777943 1106 78-79 0.5334580888930596 1106 80-81 0.4840327411327614 1106 82-83 0.43480530942217044 1106 84-85 0.5321390643781605 1106 86-87 0.4027434990096417 1106 88-89 0.3294938121416777 1106 90-91 0.1824866978502797 1106 92-93 0.542777038021871 1106 94-95 0.8591883522636365 1106 96-97 0.6195861736489583 1106 98-99 0.1343036075652293 1106 100-101 -0.19205681974031918 1107 1 0.12064996582451215 1107 2 0.17058065698243752 1107 3 0.19364079462339134 1107 4 0.11917999586888328 1107 5 0.07850152139029376 1107 6 0.03307024640994882 1107 7 0.10379152047062945 1107 8 0.1748880721483559 1107 9 -0.04487880128304056 1107 10-11 0.22750618792772315 1107 12-13 0.1831753028577907 1107 14-15 0.31425572055273676 1107 16-17 0.23617336789799737 1107 18-19 0.1742456489343951 1107 20-21 0.035604173017972585 1107 22-23 0.22882474546028675 1107 24-25 0.08153727939510702 1107 26-27 0.11612069041690631 1107 28-29 0.24357839352548183 1107 30-31 0.1644171206280518 1107 32-33 0.3020110774752496 1107 34-35 0.1498029952857749 1107 36-37 0.06516633055587562 1107 38-39 0.3309995892234596 1107 40-41 0.3505188527469798 1107 42-43 0.5185783729908451 1107 44-45 0.36177217017480245 1107 46-47 0.3105322894745086 1107 48-49 0.3965368197637318 1107 50-51 0.2566264395370581 1107 52-53 0.27793466779998965 1107 54-55 0.5455888782932377 1107 56-57 0.6448999172558771 1107 58-59 0.27216153053458214 1107 60-61 0.16820843850742762 1107 62-63 0.22556331868862145 1107 64-65 0.29879840510306366 1107 66-67 0.7131116939631923 1107 68-69 0.4327449111164299 1107 70-71 0.5199784197613724 1107 72-73 0.6116381191169218 1107 74-75 0.4286954363661799 1107 76-77 0.41401190995807724 1107 78-79 0.6919731850572362 1107 80-81 0.8296328270895685 1107 82-83 0.6129507913175161 1107 84-85 0.9070683290893093 1107 86-87 0.552687985238645 1107 88-89 0.4769527140434704 1107 90-91 0.5902478811889864 1107 92-93 0.806431634920262 1107 94-95 0.5212312590363126 1107 96-97 0.6646561926310888 1107 98-99 0.3885924940664296 1107 100-101 1.0079467617932956 1108 1 0.03356417438898518 1108 2 -0.0036525618840528296 1108 3 0.040249347351519305 1108 4 0.08925718761553014 1108 5 0.12485178994712953 1108 6 -0.0016425592018620705 1108 7 0.07826834827140061 1108 8 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86-87 -0.04167678673525543 2316 88-89 -0.1620280598844417 2316 90-91 -0.46494071576136164 2316 92-93 -0.8097971239338477 2316 94-95 -0.5000220994200184 2316 96-97 0.2496870729828089 2316 98-99 0.13629827584527732 2316 100-101 0.08847294902941272 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 7.0 5 17.0 6 48.0 7 81.0 8 205.0 9 391.0 10 689.0 11 1209.0 12 1961.0 13 2804.0 14 3527.0 15 5125.0 16 7355.0 17 10550.0 18 9324.0 19 4887.0 20 4048.0 21 3976.0 22 4003.0 23 4287.0 24 4776.0 25 5661.0 26 6726.0 27 8551.0 28 10253.0 29 12583.0 30 15656.0 31 19444.0 32 24929.0 33 32598.0 34 44793.0 35 64254.0 36 97679.0 37 142272.0 38 94530.0 39 5541.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.371231229671366 19.928287376042118 15.358020768244781 20.342460626041735 2 15.67520260507513 22.042179734299445 39.92045478844456 22.362162872180864 3 19.21361030884062 27.520126340419615 30.68031688963083 22.58594646110893 4 12.622911760123898 17.911286652065407 37.95686213416664 31.508939453644054 5 12.873201576198184 39.22045392064025 33.16385129975257 14.742493203408987 6 29.67845300660872 37.86627078493633 17.4467461179306 15.008530090524344 7 26.85397737425944 35.42744382893389 20.69642805323021 17.022150743576468 8 27.023510059703 33.72020386687255 19.687937673064617 19.568348400359838 9 27.882781130248453 16.5873223152361 20.577144244823526 34.952752309691924 10-11 24.601084548995647 27.833040556022304 26.624914076223938 20.940960818758114 12-13 26.66665139345176 25.631814717575345 27.613437985401863 20.08809590357103 14-15 23.457825308022564 25.905434362595287 27.624129235835237 23.012611093546916 16-17 20.891543373639347 29.59467942285576 27.796792930334284 21.71698427317061 18-19 20.918898403260528 26.94074980851192 32.07087388056795 20.0694779076596 20-21 22.43488606875738 25.693828208883872 31.959091971280074 19.91219375107868 22-23 21.639586636241724 26.100231241771365 32.02049406927478 20.239688052712125 24-25 21.69136993342452 26.104506007220422 32.19092658062338 20.013197478731666 26-27 21.442645336005267 26.266788044117444 32.22403304073425 20.066533579143037 28-29 20.888565096732904 26.476652600035738 32.25428070030745 20.380501602923903 30-31 21.137102495626028 26.344781133467382 32.165082924065125 20.35303344684146 32-33 21.008703700733825 26.501115359736925 31.996352647652476 20.493828291876774 34-35 21.390824768878076 26.343622898214715 31.926670240598952 20.338882092308257 36-37 21.689704340308406 26.14502927728225 31.717984965369027 20.447281417040315 38-39 21.388354690247102 26.30734403903053 31.89384832288782 20.41045294783455 40-41 21.289186446191877 26.444679822555113 31.79666385892994 20.469469872323074 42-43 21.250463923902736 26.781658701685096 31.22066588162342 20.74721149278875 44-45 21.54416784652252 26.988152567198327 30.746127094530507 20.721552491748646 46-47 21.96688761951309 27.24172954149739 29.89492012096405 20.896462718025475 48-49 22.103476927400585 27.44312037585762 29.375943157032975 21.07745953970882 50-51 22.011121302541127 27.76957626477895 29.079968443055833 21.13933398962409 52-53 22.192286721008767 27.964187365691167 28.909370270076256 20.93415564322381 54-55 21.73184882311657 27.81463427300213 28.962382955065287 21.491133948816014 56-57 22.065724831205053 28.195725006854133 28.548932397251015 21.1896177646898 58-59 22.18460763050982 27.619436722974005 28.769206097076705 21.42674954943947 60-61 22.070267454985053 27.443499351638163 29.378122484638634 21.108110708738145 62-63 21.945471208186955 27.51878723079273 29.525584237055142 21.010157323965174 64-65 21.91118320068546 27.721419614778974 29.229573220555917 21.13782396397965 66-67 21.827837591394168 27.873102812046085 28.899039129764066 21.40002046679568 68-69 21.934074292425816 27.828960330636576 29.025013096861795 21.211952280075817 70-71 21.98394921911095 27.984320361917693 28.74271937709102 21.289011041880336 72-73 21.89186258383086 27.860100406114785 28.460490484023452 21.787546526030905 74-75 21.92042349570288 28.350752434932286 28.272095378172434 21.456728691192396 76-77 21.99946238283535 28.159531784323878 28.39038642761031 21.450619405230466 78-79 21.89001116506115 27.934952445392767 28.270438858689428 21.904597530856655 80-81 21.9157567398732 28.403004018493316 28.117845256696345 21.56339398493714 82-83 21.926182554739842 28.276370901958625 28.1322463270001 21.66520021630143 84-85 21.767534858926076 28.162315776771514 27.98795431928386 22.082195045018555 86-87 21.893071309214324 27.848412550519473 28.043387943754222 22.21512819651198 88-89 22.01261415782283 27.758631091249132 27.91678535236479 22.311969398563246 90-91 21.802893224984402 27.83547274361518 28.251517977079327 22.11011605432109 92-93 21.74848031279592 28.009057030271556 27.962168188899412 22.280294468033112 94-95 21.55777747061622 28.204685673178915 27.78146456496887 22.456072291235994 96-97 21.777284609457276 28.05390367078622 27.662669814051277 22.50614190570523 98-99 21.676402328616582 28.33600749018479 27.272886951514646 22.71470322968398 100-101 21.51809994661999 28.38289121093198 27.288410123415495 22.810598719032534 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 621.0 1 441.5 2 298.0 3 351.0 4 302.5 5 279.5 6 486.5 7 1053.0 8 1744.0 9 2047.0 10 1784.5 11 1478.5 12 1481.5 13 1544.5 14 1660.5 15 1758.0 16 1767.0 17 1864.0 18 1970.0 19 2059.5 20 2192.0 21 2389.5 22 2634.0 23 2964.0 24 3422.0 25 3991.5 26 4888.0 27 5811.5 28 6513.5 29 7349.5 30 8467.0 31 9843.0 32 11245.0 33 12415.0 34 13778.0 35 14916.0 36 16448.5 37 18149.5 38 18847.5 39 18874.5 40 18776.0 41 19462.5 42 20135.5 43 20701.5 44 22055.5 45 23120.0 46 23066.5 47 23655.5 48 27261.5 49 26977.0 50 23004.0 51 21181.5 52 20027.5 53 19674.0 54 19151.5 55 17694.0 56 15728.5 57 14071.5 58 13266.5 59 12324.5 60 10504.5 61 8565.0 62 6883.5 63 5234.0 64 3698.0 65 2660.0 66 1873.5 67 1403.5 68 982.0 69 557.0 70 358.0 71 256.5 72 185.5 73 134.5 74 97.5 75 79.5 76 53.5 77 25.5 78 12.5 79 7.5 80 4.0 81 2.5 82 2.5 83 0.5 84 1.0 85 2.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02810271542487793 2 0.0029019108319167427 3 3.0546429809649923E-4 4 4.581964471447488E-4 5 1.5273214904824961E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.013898625563390716 12-13 0.0 14-15 0.0 16-17 0.0 18-19 7.636607452412481E-5 20-21 6.109285961929985E-4 22-23 0.0022909822357237443 24-25 0.010767616507901596 26-27 0.005192893067640487 28-29 0.0 30-31 0.002367348310247869 32-33 0.0029782769064408674 34-35 0.0 36-37 0.008018437825033104 38-39 0.011302179029570472 40-41 3.81830372620624E-4 42-43 0.0 44-45 0.0 46-47 1.5273214904824961E-4 48-49 0.0038183037262062404 50-51 0.007865705675984856 52-53 0.0 54-55 0.0037419376516821153 56-57 0.004047401949778615 58-59 1.5273214904824961E-4 60-61 0.0021382500866754944 62-63 0.006262018110978235 64-65 0.0 66-67 0.003360107279061492 68-69 6.109285961929985E-4 70-71 9.927589688136225E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.003054642980964992 80-81 0.002749178682868493 82-83 0.015120482755776713 84-85 0.0101566879117086 86-87 0.006567482409074734 88-89 7.636607452412481E-5 90-91 6.872946707171233E-4 92-93 1.5273214904824961E-4 94-95 7.636607452412481E-5 96-97 0.002672812608344368 98-99 0.002672812608344368 100-101 2.290982235723744E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 654741.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.236875426057686 #Duplication Level Percentage of deduplicated Percentage of total 1 80.32481133184648 47.581908424861766 2 11.403311082317032 13.509930360555941 3 3.542647041911219 6.2956602450056 4 1.502999392521955 3.5613195112105367 5 0.7918104929729605 2.3452189766642295 6 0.5046170622489276 1.793516283258175 7 0.3226055107745987 1.337709971746023 8 0.23365430526038583 1.1072760778777226 9 0.19071596681529407 1.0167676171197955 >10 1.0751271910475837 11.831324519658171 >50 0.07111338044871887 2.822702188772438 >100 0.034010675508525996 3.641087329735643 >500 0.0010306265305486475 0.4751301499575063 >1k 0.0012882831631858094 1.7478928613277747 >5k 2.576566326371619E-4 0.9325554822487515 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6110 0.9331934306848051 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4751 0.7256304401282339 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3246 0.4957685558106183 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1230 0.18786054332934704 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1130 0.17258732842452207 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1095 0.16724170320783333 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 829 0.12661495156099892 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 824 0.1258512908157577 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 777 0.11867287981048993 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 683 0.1043160577999545 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.5273214904824961E-4 0.0 0.0 0.0 0.0 7 3.0546429809649923E-4 0.0 0.0 0.0 0.0 8 3.0546429809649923E-4 0.0 0.0 0.0 0.0 9 3.0546429809649923E-4 0.0 0.0 0.0 0.0 10-11 3.0546429809649923E-4 0.0 0.0 0.0 0.0 12-13 3.0546429809649923E-4 0.0 0.0 0.0 0.0 14-15 3.0546429809649923E-4 0.0 0.0 0.0 0.0 16-17 4.581964471447488E-4 0.0 0.0 0.0 0.0 18-19 4.581964471447488E-4 0.0 0.0 0.0 0.0 20-21 4.581964471447488E-4 0.0 0.0 0.0 0.0 22-23 4.581964471447488E-4 0.0 0.0 7.636607452412481E-5 0.0 24-25 4.581964471447488E-4 0.0 0.0 4.581964471447488E-4 0.0 26-27 4.581964471447488E-4 0.0 0.0 7.63660745241248E-4 0.0 28-29 4.581964471447488E-4 0.0 0.0 0.0025200804592961186 0.0 30-31 4.581964471447488E-4 0.0 0.0 0.005040160918592237 0.0 32-33 4.581964471447488E-4 0.0 0.0 0.007483875303364231 0.0 34-35 4.581964471447488E-4 0.0 0.0 0.010233053986232723 0.0 36-37 4.581964471447488E-4 0.0 0.0 0.013364063041721842 0.0 38-39 4.581964471447488E-4 0.0 0.0 0.01573141135196971 0.0 40-41 4.581964471447488E-4 0.0 0.0 0.02046610797246545 0.0 42-43 4.581964471447488E-4 0.0 0.0 0.02840817972297443 0.0 44-45 6.109285961929985E-4 0.0 0.0 0.04131404631755152 0.0 46-47 7.63660745241248E-4 0.0 0.0 0.05643452907332823 0.0 48-49 7.63660745241248E-4 0.0 0.0 0.09087562868370852 0.0 50-51 7.63660745241248E-4 0.0 0.0 0.1252403622195647 0.0 52-53 7.63660745241248E-4 0.0 0.0 0.1631943012580547 0.0 54-55 7.63660745241248E-4 0.0 0.0 0.2431495812848134 0.0 56-57 7.63660745241248E-4 0.0 0.0 0.3134827359215323 0.0 58-59 7.63660745241248E-4 0.0 0.0 0.3992418376121245 0.0 60-61 7.63660745241248E-4 0.0 0.0 0.49546309151252177 0.0 62-63 7.63660745241248E-4 0.0 0.0 0.6281109629609265 0.0 64-65 7.63660745241248E-4 0.0 0.0 0.7079898769131611 0.0 66-67 7.63660745241248E-4 0.0 0.0 0.8546127399994807 0.0 68-69 7.63660745241248E-4 0.0 0.0 1.0535463641348257 0.0 70-71 8.400268197653728E-4 0.0 0.0 1.1648117347164757 0.0 72-73 9.163928942894976E-4 0.0 0.0 1.344806572369838 0.0 74-75 9.163928942894976E-4 0.0 0.0 1.5827632605870108 0.0 76-77 9.163928942894976E-4 0.0 0.0 1.7373281954238393 0.0 78-79 9.163928942894976E-4 0.0 0.0 1.88211827272158 0.0 80-81 9.163928942894976E-4 0.0 0.0 2.1174021483304086 0.0 82-83 9.163928942894976E-4 0.0 0.0 2.408433258341848 0.0 84-85 9.927589688136225E-4 0.0 0.0 2.7555170670539955 0.0 86-87 0.001145491117861872 0.0 0.0 3.275646400637809 0.0 88-89 0.001221857192385997 0.0 0.0 3.8047105649409465 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5275 0.0 28.819546 1 GTATCAA 7575 0.0 28.347542 1 TCGCGAT 35 0.00839046 27.143871 50-51 TCAATCG 35 0.008398279 27.138689 14-15 AGTACTT 4865 0.0 23.477896 12-13 GAGTACT 4685 0.0 23.416895 12-13 TATCAAC 9140 0.0 23.330662 2 ATCAACG 9150 0.0 23.253262 3 TCAACGC 9200 0.0 23.126884 4 CAACGCA 9230 0.0 23.056997 5 AACGCAG 9485 0.0 22.487202 6 GTACATG 4650 0.0 21.965702 1 GTACTTT 5050 0.0 21.724388 14-15 TACTTTT 5380 0.0 21.142199 14-15 TACATGG 4850 0.0 20.759699 2 ACGCAGA 10385 0.0 20.492641 7 ATTACGG 70 4.8497616E-4 20.35868 80-81 CGCAGAG 10370 0.0 20.339048 8 ACATGGG 4845 0.0 19.898905 3 TTGCGAT 60 0.004763868 19.79165 44-45 >>END_MODULE