FastQCFastQC Report
Thu 26 May 2016
SRR936988_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936988_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679198
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60980.8978236096101578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41590.6123398478794108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29260.43080221084278814No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8970.13206752670060867No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA8550.12588376290860692No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA7020.10335719480917199No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6890.10144317268307622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38750.033.255511
GTATCAA59800.032.761371
GTATAGG2250.029.587721
GTACATG46400.027.5676861
TACATGG46150.027.3048742
TATCAAC72500.026.8257142
ATCAACG73400.026.3672223
TCAACGC73600.026.295574
CAACGCA74450.025.9953525
AACGCAG76050.025.4428146
GTCTAAT1501.0490439E-625.3609051
ACATGGG47450.025.2540113
CATGGGA33750.023.5292844
GAGTACT41150.023.0994712-13
TAGACTG2704.0017767E-1122.8952625
ACGCAGA85000.022.6419587
CGCAGAG85000.022.5860528
TCTAGAC1903.2485877E-722.5244883
ATGGGAG12400.022.2419225
GCAGAGT85650.022.1372389
Produced by FastQC (version 0.11.5)