##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936988_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 679198 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79556476903642 33.0 31.0 34.0 30.0 34.0 2 31.864727222400536 33.0 31.0 34.0 30.0 34.0 3 31.865401547118807 33.0 31.0 34.0 30.0 34.0 4 35.48511479715783 37.0 35.0 37.0 33.0 37.0 5 35.49212306278876 37.0 35.0 37.0 33.0 37.0 6 35.574880962547006 37.0 35.0 37.0 33.0 37.0 7 35.52246178581209 37.0 35.0 37.0 33.0 37.0 8 35.490017638450055 37.0 35.0 37.0 33.0 37.0 9 37.10058627970047 39.0 37.0 39.0 33.0 39.0 10-11 36.98365277871843 39.0 37.0 39.0 33.0 39.0 12-13 36.89782360961016 39.0 37.0 39.0 33.0 39.0 14-15 38.001531953863235 40.0 38.0 41.0 33.0 41.0 16-17 37.97101655187441 40.0 38.0 41.0 33.0 41.0 18-19 37.984694448452444 40.0 38.0 41.0 33.0 41.0 20-21 37.95487103907844 40.0 38.0 41.0 33.0 41.0 22-23 37.84716975020539 40.0 38.0 41.0 33.0 41.0 24-25 37.79860143875571 40.0 37.5 41.0 32.5 41.0 26-27 37.78077085032641 40.0 37.0 41.0 33.0 41.0 28-29 37.673423655546685 40.0 37.0 41.0 32.0 41.0 30-31 37.47038345224809 40.0 37.0 41.0 32.0 41.0 32-33 37.20811015344568 39.5 36.0 41.0 31.5 41.0 34-35 37.0050007508856 39.0 36.0 41.0 31.0 41.0 36-37 36.800298292986724 39.0 35.0 41.0 31.0 41.0 38-39 36.601876772310874 39.0 35.0 41.0 30.0 41.0 40-41 36.37611197323902 39.0 35.0 41.0 30.0 41.0 42-43 36.09922953247801 39.0 35.0 41.0 30.0 41.0 44-45 35.75210542433871 39.0 35.0 40.0 28.5 41.0 46-47 35.26013327483297 38.5 35.0 40.0 26.5 41.0 48-49 34.88339629975353 38.5 35.0 40.0 24.0 41.0 50-51 34.14931875535558 38.0 34.0 39.5 23.5 40.5 52-53 34.3079595346276 38.0 34.0 40.0 23.0 40.5 54-55 34.67648461862373 39.0 35.0 40.0 23.0 41.0 56-57 34.56346013975306 38.5 34.5 40.5 23.0 41.0 58-59 34.34265413031251 38.0 34.0 40.0 22.5 41.0 60-61 34.056919337218304 38.0 34.0 40.0 22.0 41.0 62-63 33.74163940412075 37.5 34.0 40.0 20.5 41.0 64-65 33.40215445275163 37.0 33.5 40.0 19.5 41.0 66-67 33.0252511049797 36.5 33.0 39.5 18.0 41.0 68-69 32.63057900641639 36.0 33.0 39.0 14.5 41.0 70-71 32.188308858388865 35.5 32.0 39.0 11.0 40.5 72-73 31.80788150141785 35.0 32.0 38.0 8.0 40.0 74-75 31.419102382515852 35.0 31.5 37.0 7.0 39.5 76-77 30.959346170041727 35.0 31.0 37.0 2.0 39.0 78-79 30.522742999832154 35.0 31.0 36.0 2.0 39.0 80-81 30.095487619221494 35.0 30.5 36.0 2.0 37.5 82-83 29.702625007729704 34.0 30.0 35.0 2.0 37.0 84-85 29.34043239232153 34.0 29.5 35.0 2.0 37.0 86-87 28.999123230633778 34.0 29.0 35.0 2.0 36.0 88-89 28.64249747496312 34.0 29.0 35.0 2.0 36.0 90-91 28.27756780791463 34.0 29.0 35.0 2.0 35.5 92-93 27.903988233180897 34.0 28.0 35.0 2.0 35.0 94-95 27.545732614053634 34.0 27.0 35.0 2.0 35.0 96-97 27.074524659966606 34.0 26.5 35.0 2.0 35.0 98-99 26.5353114997394 34.0 25.0 35.0 2.0 35.0 100-101 24.488487893073888 31.0 13.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1496860903440087 1101 2 0.0979265354016654 1101 3 0.0549759640627876 1101 4 0.2557412536792256 1101 5 0.09712669203668867 1101 6 0.0808751729477919 1101 7 0.018336246661739608 1101 8 0.09294500831099839 1101 9 0.004611900938961355 1101 10-11 0.0710058692086264 1101 12-13 0.18412178843573201 1101 14-15 0.18340139338950934 1101 16-17 0.16328043715532914 1101 18-19 0.17510471578407305 1101 20-21 0.1860545475342974 1101 22-23 0.20037660221148457 1101 24-25 0.2865039205480997 1101 26-27 0.26058608911611003 1101 28-29 0.2903296120287564 1101 30-31 0.2574962580628508 1101 32-33 0.21672389337734188 1101 34-35 0.23275471604532072 1101 36-37 0.32054092529360645 1101 38-39 0.3107659824288973 1101 40-41 0.35160246808903395 1101 42-43 0.4495901086830827 1101 44-45 0.44052856515844496 1101 46-47 0.3536678611433288 1101 48-49 0.15189532444652798 1101 50-51 0.3087968668604404 1101 52-53 0.3272602953506265 1101 54-55 0.47718197195440126 1101 56-57 0.3351096698581699 1101 58-59 0.348869456109135 1101 60-61 0.5287277063212414 1101 62-63 0.35691990451742583 1101 64-65 0.3616564025762159 1101 66-67 0.3185288041464247 1101 68-69 0.3459319575142743 1101 70-71 0.3494433414754212 1101 72-73 0.22095879769457127 1101 74-75 0.17570173975771297 1101 76-77 0.4172554704411837 1101 78-79 0.21411151628732128 1101 80-81 0.41309325747703696 1101 82-83 0.4258177293590215 1101 84-85 0.259742754076818 1101 86-87 0.3640410167728483 1101 88-89 0.405689117718083 1101 90-91 0.5344798274270239 1101 92-93 0.5200684512532057 1101 94-95 0.3871120584185448 1101 96-97 0.3876013756041239 1101 98-99 0.2129831507659432 1101 100-101 0.5990624106427553 1102 1 0.09738492121254438 1102 2 0.00723626588951376 1102 3 0.07663982387531476 1102 4 0.09155165835358048 1102 5 0.15616972625503678 1102 6 -0.013739457711722025 1102 7 -0.11326929195642066 1102 8 -0.12684737473524166 1102 9 0.07125239436767572 1102 10-11 -0.04939463548359413 1102 12-13 -0.10589835593223995 1102 14-15 -0.13561662282345566 1102 16-17 -0.010491000080342872 1102 18-19 -0.27647353046458534 1102 20-21 -0.28346572408975845 1102 22-23 -0.324548804060349 1102 24-25 -0.25989608595008207 1102 26-27 -0.269968639124464 1102 28-29 -0.42744643891700207 1102 30-31 -0.4138378189514498 1102 32-33 -0.21317235229729903 1102 34-35 -0.336415736715594 1102 36-37 -0.3893314395577221 1102 38-39 -0.18012708455112403 1102 40-41 -0.24258247020529922 1102 42-43 -0.43939249243135947 1102 44-45 -0.1631783776088085 1102 46-47 -0.22933370383579188 1102 48-49 -0.11079914722409967 1102 50-51 -0.1912735301243984 1102 52-53 -0.06763944309749803 1102 54-55 -0.09230499648067791 1102 56-57 0.0059650473755397115 1102 58-59 -0.30669759160377197 1102 60-61 -0.3021443498663885 1102 62-63 -0.17314339861739825 1102 64-65 0.013978690803135407 1102 66-67 -0.18847948645232293 1102 68-69 -0.14943429633725458 1102 70-71 -0.06553280477702828 1102 72-73 0.13190715338345882 1102 74-75 0.08796462375972425 1102 76-77 0.03260363768275809 1102 78-79 0.13336698694531535 1102 80-81 -0.07660495606409157 1102 82-83 -0.03014462449954536 1102 84-85 -0.034767905651229114 1102 86-87 0.07382159478624217 1102 88-89 0.34646548815784683 1102 90-91 -0.001813338108778595 1102 92-93 0.0622451801760775 1102 94-95 -0.06720896266264376 1102 96-97 -0.3731525761035428 1102 98-99 -0.19411340682149358 1102 100-101 0.11925199513808948 1103 1 0.25955723556234744 1103 2 0.34341961439825397 1103 3 0.30935842109678546 1103 4 0.3576265370825311 1103 5 0.04405721463554357 1103 6 0.08201724511153685 1103 7 0.3054538800946105 1103 8 0.24517827222877742 1103 9 0.08844702766748469 1103 10-11 0.20127239496979143 1103 12-13 0.2085850087565504 1103 14-15 0.3840021625307415 1103 16-17 0.3738149868550593 1103 18-19 0.26154609498314585 1103 20-21 0.298237495740004 1103 22-23 0.4006206898848248 1103 24-25 0.5206681853290576 1103 26-27 0.43726701535852186 1103 28-29 0.4682563304094316 1103 30-31 0.5288372190399357 1103 32-33 0.5161896778864232 1103 34-35 0.629790168685048 1103 36-37 0.5260706981122922 1103 38-39 0.5830588622831385 1103 40-41 0.5090185615107643 1103 42-43 0.5751203770527553 1103 44-45 0.36428487623078354 1103 46-47 0.23580019400147734 1103 48-49 0.2508931590039509 1103 50-51 0.3081347876898306 1103 52-53 0.3804251375061156 1103 54-55 0.4288965783774259 1103 56-57 0.5910660775053245 1103 58-59 0.4501571972148639 1103 60-61 0.5006291008939172 1103 62-63 0.6388088987909342 1103 64-65 0.6419181456941061 1103 66-67 0.4287935090458177 1103 68-69 0.6798192323410959 1103 70-71 0.6567258666188707 1103 72-73 0.5537990770009387 1103 74-75 0.4264341180221507 1103 76-77 0.5874904883789789 1103 78-79 0.7762031909154565 1103 80-81 0.691347442866121 1103 82-83 0.8214408616792035 1103 84-85 0.6462062602037904 1103 86-87 0.9312985223535577 1103 88-89 0.6055036318303344 1103 90-91 0.6593117146350096 1103 92-93 0.6416936625826537 1103 94-95 0.9166757084857906 1103 96-97 0.7524620278183747 1103 98-99 0.7767790021974541 1103 100-101 0.9860540243065934 1104 1 0.0710145573421812 1104 2 0.1761039972572469 1104 3 0.06713648937197902 1104 4 0.16730119035311475 1104 5 0.07535701419232765 1104 6 -0.10540027437253485 1104 7 0.01980517861804998 1104 8 0.08702810789024085 1104 9 0.11627330813858805 1104 10-11 0.18549955253558892 1104 12-13 0.02741888988501273 1104 14-15 -0.1705952703021083 1104 16-17 0.17505911984477507 1104 18-19 0.17220802446696837 1104 20-21 0.09265053015149505 1104 22-23 0.05008666745012391 1104 24-25 0.033619182565189476 1104 26-27 -0.01408426449008715 1104 28-29 -0.04955946103002162 1104 30-31 0.08565578991716194 1104 32-33 0.06472595997600905 1104 34-35 0.037631809206956746 1104 36-37 -0.1367684057446894 1104 38-39 0.032953894154850616 1104 40-41 0.22355038342755762 1104 42-43 0.2156162094523424 1104 44-45 -0.0018955444509742847 1104 46-47 -0.22696335459044548 1104 48-49 -0.1964706547706072 1104 50-51 -0.2626070389579098 1104 52-53 -0.2209282432613051 1104 54-55 0.047321480645798886 1104 56-57 -0.03238430034880935 1104 58-59 0.027565161408979577 1104 60-61 -0.03256035840738747 1104 62-63 -0.3868079154069193 1104 64-65 -0.13408422600978298 1104 66-67 -0.297048648146486 1104 68-69 -0.390472453000406 1104 70-71 -0.39923249472671785 1104 72-73 -0.19271223061092613 1104 74-75 -0.1477913432462401 1104 76-77 -0.015205535126412428 1104 78-79 0.05747303292636019 1104 80-81 -0.3132570708412068 1104 82-83 -0.3311570411369651 1104 84-85 -0.2432628453961705 1104 86-87 0.16770285429250364 1104 88-89 -0.08891461597225714 1104 90-91 -0.06170533237576947 1104 92-93 -0.287931782500884 1104 94-95 0.03439174518806354 1104 96-97 -0.032399607225574556 1104 98-99 -0.3942435694516604 1104 100-101 -0.1492183014572035 1105 1 -0.11951574568812262 1105 2 -0.012911154257906787 1105 3 0.17509103482652577 1105 4 0.09021785701978047 1105 5 0.21721307479838714 1105 6 0.13020578623352463 1105 7 0.14329002710289984 1105 8 0.254641744253874 1105 9 0.10812936874464896 1105 10-11 -0.024822417161381338 1105 12-13 -0.07845232223620258 1105 14-15 0.196828972122141 1105 16-17 0.14030533196598327 1105 18-19 -0.05563288462393956 1105 20-21 -0.0413456819697231 1105 22-23 0.053590455328908604 1105 24-25 0.11714190983791184 1105 26-27 0.2061808870250701 1105 28-29 0.18169053896998122 1105 30-31 0.17987927476564636 1105 32-33 0.15269944482449915 1105 34-35 -0.1310234938233492 1105 36-37 0.04201947304319731 1105 38-39 0.27054859112455176 1105 40-41 0.1235503834275491 1105 42-43 0.08313514884628148 1105 44-45 0.07016884948841806 1105 46-47 0.16593437268227973 1105 48-49 0.17199525432030072 1105 50-51 -0.06620552380639566 1105 52-53 0.2598482718902133 1105 54-55 0.04372299579731731 1105 56-57 0.16486948752997677 1105 58-59 0.2677166765604966 1105 60-61 0.2951858537138179 1105 62-63 0.36480193307792774 1105 64-65 0.5170521376265782 1105 66-67 0.3308869579141174 1105 68-69 0.40261466821171865 1105 70-71 0.5130781113338898 1105 72-73 0.4740491330254386 1105 74-75 0.45902683857193693 1105 76-77 0.5389611315402512 1105 78-79 0.5486662147445465 1105 80-81 0.2961179291587932 1105 82-83 0.4742406861357651 1105 84-85 0.19726745763413334 1105 86-87 0.5729111876258379 1105 88-89 0.7040778082701671 1105 90-91 0.6014575464121066 1105 92-93 0.41017427810517404 1105 94-95 0.47264932094564216 1105 96-97 0.538717817016849 1105 98-99 0.7843549153968254 1105 100-101 0.7004976076337002 1106 1 0.13176718988919944 1106 2 0.04287585084624723 1106 3 0.1541657347851384 1106 4 0.2988811209182529 1106 5 0.12526763404793684 1106 6 0.12362556827242344 1106 7 0.16432223844239502 1106 8 0.10964760713151378 1106 9 0.0033338797116826413 1106 10-11 0.19106856780206272 1106 12-13 0.08751625963715526 1106 14-15 0.041850699256912094 1106 16-17 0.022921037580552195 1106 18-19 0.08901120421590036 1106 20-21 0.16702788679838676 1106 22-23 -0.2136214014789246 1106 24-25 -0.22401980212235628 1106 26-27 -0.42220233824738074 1106 28-29 -0.3734662067880592 1106 30-31 -0.22596980786644139 1106 32-33 -0.3133053489401618 1106 34-35 -0.15904977331017278 1106 36-37 -0.41417017380464927 1106 38-39 -0.08639374574949699 1106 40-41 -0.1964127149308439 1106 42-43 -0.14305898137857298 1106 44-45 -0.1824599613820439 1106 46-47 0.07242923404247392 1106 48-49 -0.11062045129426679 1106 50-51 -0.014587197140649266 1106 52-53 -0.26803316003142896 1106 54-55 -0.08045189039388134 1106 56-57 -0.1846847613319298 1106 58-59 -0.13927825377471947 1106 60-61 -0.11411111828231668 1106 62-63 -0.09832487751663166 1106 64-65 -0.10952480402054476 1106 66-67 0.013977729054964527 1106 68-69 0.3139558876902626 1106 70-71 0.272953611402869 1106 72-73 0.15442401231913294 1106 74-75 0.1306565461178053 1106 76-77 0.1604796168485727 1106 78-79 0.24538472764287533 1106 80-81 0.23311786018376068 1106 82-83 0.4107017497307446 1106 84-85 0.24503008006481153 1106 86-87 0.257694433590796 1106 88-89 0.18648400222795303 1106 90-91 0.13305931907040502 1106 92-93 0.1570290166898083 1106 94-95 0.38272571079251705 1106 96-97 0.31577413512969343 1106 98-99 0.5431414721632777 1106 100-101 1.0285195761810897 1107 1 0.18151408385733347 1107 2 0.24298373210573132 1107 3 0.3574418870992524 1107 4 0.29792382292887254 1107 5 0.37566477934384324 1107 6 0.2419600475971606 1107 7 0.3260315043756279 1107 8 0.33507498289024085 1107 9 0.30898164147192375 1107 10-11 0.4862216169295266 1107 12-13 0.41934597321834133 1107 14-15 0.21129985280395402 1107 16-17 0.4682350194659861 1107 18-19 0.4276235074215151 1107 20-21 0.5115662498484639 1107 22-23 0.710657216692546 1107 24-25 0.274741341656096 1107 26-27 0.4396325915705219 1107 28-29 0.45088443101543163 1107 30-31 0.7418466043110996 1107 32-33 0.688335098233587 1107 34-35 0.4496228129948321 1107 36-37 0.3809576832704664 1107 38-39 0.5551189036245532 1107 40-41 0.6696618891093706 1107 42-43 0.5728013420281002 1107 44-45 0.7566567756247835 1107 46-47 0.715508236318648 1107 48-49 0.7770733793202993 1107 50-51 0.9036453284663324 1107 52-53 0.9012900332538507 1107 54-55 1.0224339332973145 1107 56-57 0.6568616750299796 1107 58-59 0.8221023299695887 1107 60-61 0.9545075866683632 1107 62-63 0.8114638648961119 1107 64-65 0.7115301205811235 1107 66-67 0.9162562760959361 1107 68-69 0.6715244693480784 1107 70-71 0.6330532533793445 1107 72-73 0.6568083659799839 1107 74-75 0.5005573783446664 1107 76-77 0.5481230633584353 1107 78-79 0.5439787147445436 1107 80-81 0.48894463370424646 1107 82-83 0.723352902044855 1107 84-85 0.5654492758159506 1107 86-87 0.7509474092167459 1107 88-89 0.8550366150883484 1107 90-91 0.913584677093926 1107 92-93 0.9955968633324481 1107 94-95 1.2118005993547314 1107 96-97 0.8550885556532144 1107 98-99 0.9324905688059175 1107 100-101 1.0076531474064296 1108 1 0.008472932300556124 1108 2 0.10440486305811092 1108 3 0.1049736522091429 1108 4 0.02435039115231774 1108 5 0.19478966487498184 1108 6 0.1497196057473431 1108 7 0.2345279558408322 1108 8 0.05577810789024085 1108 9 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-3.040736434673061 2316 78-79 -3.5563534702948267 2316 80-81 -2.9220728367681943 2316 82-83 -3.321907309569319 2316 84-85 -3.5204267084360197 2316 86-87 -3.23074396627538 2316 88-89 -3.2262103091242516 2316 90-91 -3.3136775645399332 2316 92-93 -3.4098149846006223 2316 94-95 -3.747022296806687 2316 96-97 -3.981356449268045 2316 98-99 -4.156576544875186 2316 100-101 -4.134286751707741 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1355.0 3 467.0 4 438.0 5 475.0 6 485.0 7 628.0 8 818.0 9 1038.0 10 1315.0 11 1853.0 12 2605.0 13 3601.0 14 3998.0 15 5285.0 16 7072.0 17 9697.0 18 8375.0 19 4600.0 20 4170.0 21 4313.0 22 4513.0 23 5011.0 24 5545.0 25 6194.0 26 7303.0 27 8527.0 28 9907.0 29 12123.0 30 14670.0 31 18478.0 32 23272.0 33 30423.0 34 41871.0 35 60277.0 36 90242.0 37 133286.0 38 122222.0 39 22739.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.382322010881566 19.18252703455061 15.173371667260724 21.26177928730709 2 14.933136966126655 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21.91010739382154 25.584773353368394 31.862008514901074 20.643110737908987 22-23 21.287748465317954 25.80327791589589 31.870795089017452 21.038178529768707 24-25 20.97416246102651 26.017912161938312 32.10289370129464 20.905031675740542 26-27 20.879569064458494 25.95802103586197 32.04056106429359 21.12184883538595 28-29 20.51170676653535 26.104731075293913 32.300633799032184 21.08292835913855 30-31 20.659808750873314 26.06511069082471 32.2197870478005 21.055293510501475 32-33 20.526346012833613 26.244047741749295 31.890631579877315 21.338974665539777 34-35 20.950379150408597 26.17227416623721 31.708164617536628 21.169182065817566 36-37 21.193421545696758 25.86318470043355 31.67038819476807 21.27300555910162 38-39 20.993151214383996 26.06499242091283 31.724534966499622 21.217321398203545 40-41 20.86562812886507 26.120943518463985 31.834545615171685 21.178882737499265 42-43 20.659852587377166 26.46521845396374 31.223147845496495 21.6517811131626 44-45 20.8814636879158 26.598138808298433 30.91181608640311 21.608581417382656 46-47 21.24571733135451 26.76780169348534 30.153152795016425 21.83332818014373 48-49 21.290051862676172 26.99119181092392 29.623415869463592 22.09534045693631 50-51 21.394044956558485 27.27620735907089 29.45328914337541 21.876458540995216 52-53 21.475577713499455 27.367081171056494 29.240338680835055 21.917002434608992 54-55 20.9869353467158 27.3096532657952 29.391015612058208 22.312395775430797 56-57 21.300736153229423 27.681319789704162 28.882105785491476 22.135838271574944 58-59 21.51220316622691 27.139308683565776 29.103108509234826 22.245379640972484 60-61 21.376875876513306 27.033382043854193 29.716607833682406 21.873134245950094 62-63 21.419184605315372 27.009534803763856 29.731345437304483 21.83993515361628 64-65 21.231527383716564 27.188775893318574 29.684428745798247 21.895267977166615 66-67 21.127010493887155 27.388588965366935 29.2035189717581 22.28088156898781 68-69 21.12478448418716 27.403889705985247 29.423864827303802 22.047460982523795 70-71 21.17464040117146 27.372600955787462 29.19703835524416 22.255720287796915 72-73 21.186811892413193 27.28931162554432 29.015309070965035 22.50856741107745 74-75 21.068922154454185 27.824415341165103 28.816148687849168 22.29051381653154 76-77 21.100403357037983 27.4821037006057 28.992814972660376 22.424677969695946 78-79 21.093848373122555 27.31925854417167 29.005817982063736 22.581075100642032 80-81 20.996656789781476 27.745992641695977 28.79116944579918 22.46618112272337 82-83 21.065863130873534 27.69230882519709 28.73200107805755 22.50982696587182 84-85 21.040301389812264 27.43062534557564 28.44347947087446 23.085593793737637 86-87 21.104016365616594 27.407989431275677 28.74807893258241 22.73991527052532 88-89 21.237867205975604 27.150355689073468 28.60360277422011 23.008174330730814 90-91 20.930515161580402 27.122116766226718 29.003164661840746 22.944203410352134 92-93 20.982796169332417 27.43767579259953 28.77780551916033 22.801722518907717 94-95 20.793323565575278 27.361159172734702 28.63459995140663 23.21091731028339 96-97 20.65966495452618 27.46402443334433 28.49830356722115 23.378007044908344 98-99 20.75099847623284 27.547927963889148 28.24647467740546 23.454598882472553 100-101 20.653541705960016 27.583120166918146 28.422057714070164 23.341280413051678 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 364.0 1 270.5 2 238.5 3 302.5 4 268.5 5 278.0 6 474.5 7 1022.0 8 1762.0 9 2096.5 10 1785.5 11 1454.0 12 1514.5 13 1580.0 14 1604.0 15 1657.0 16 1694.0 17 1788.5 18 1893.0 19 1945.0 20 2082.5 21 2287.0 22 2541.0 23 2930.5 24 3385.0 25 4054.0 26 4987.0 27 5994.0 28 6722.5 29 7653.5 30 8873.0 31 10203.5 32 11688.5 33 13061.0 34 14600.0 35 15688.5 36 17265.5 37 18958.5 38 19563.5 39 19664.0 40 19655.0 41 20434.0 42 21058.5 43 21711.5 44 22860.0 45 23815.5 46 23965.5 47 24531.5 48 28465.5 49 28141.5 50 24002.5 51 22294.5 52 20986.5 53 20751.0 54 20219.0 55 18482.0 56 16586.0 57 14792.5 58 13629.5 59 12355.5 60 10461.0 61 8766.5 62 7084.5 63 5260.5 64 3692.0 65 2714.0 66 2010.0 67 1493.5 68 1012.0 69 600.5 70 382.5 71 268.0 72 186.5 73 154.0 74 116.5 75 77.0 76 56.0 77 31.5 78 15.5 79 10.5 80 9.5 81 9.0 82 9.0 83 6.0 84 3.5 85 4.0 86 2.0 87 1.0 88 2.0 89 2.0 90 1.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03710258275201046 2 0.029152029305151077 3 0.02532398505295952 4 0.05108966751963345 5 0.10129594021183808 6 0.12073062641527214 7 0.061984870391255574 8 0.0909896672251685 9 0.07729704740002179 10-11 0.0777387448137362 12-13 0.07361623561906837 14-15 0.06875756406820986 16-17 0.05373985200191991 18-19 0.06220571909811277 20-21 0.057126198840397054 22-23 0.03386346838477145 24-25 0.007435239797525905 26-27 0.0036071955453343507 28-29 0.005815682613906401 30-31 0.004637822844001308 32-33 0.0064782287344780165 34-35 0.007067158619430564 36-37 0.006110147556382674 38-39 0.0039016604878106234 40-41 0.005594833907049196 42-43 0.002355719539810188 44-45 0.001398708476762299 46-47 0.0010306272986669574 48-49 7.361623561906837E-5 50-51 0.0013250922411432308 52-53 0.004932287786477581 54-55 0.011116051578479324 56-57 0.008760332038669136 58-59 0.004416974137144102 60-61 0.0022821033041911195 62-63 0.008024169682478453 64-65 0.0025029520110483247 66-67 0.020023616088386598 68-69 0.0446850550207745 70-71 0.05683173389792078 72-73 0.0037544280165724866 74-75 0.012367527584003486 76-77 0.0216431732720061 78-79 0.026869926000959957 80-81 0.0749413278602116 82-83 0.029299261776389213 84-85 0.011410516520955597 86-87 0.0314341326093422 88-89 0.04483228749201264 90-91 0.04335996277963127 92-93 0.04542121737696518 94-95 0.013692619825146716 96-97 0.01855129137600523 98-99 0.04247656795220245 100-101 0.07979999941107012 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 679198.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.14494501877448 #Duplication Level Percentage of deduplicated Percentage of total 1 81.28626280405105 49.70244069935353 2 11.00673371220187 13.460122553377495 3 3.2398330368663695 5.942982387276099 4 1.4438619793529128 3.53139445368931 5 0.7587101105179003 2.3195643996402655 6 0.43781537119441677 1.6062118080034171 7 0.30826382041752676 1.319414204549493 8 0.2186302781310643 1.069450906861064 9 0.17251765627875976 0.9493724347139336 >10 1.0306545276513122 11.582248252118521 >50 0.0670505744570486 2.7981871924982076 >100 0.02653068436406712 2.784019726794612 >500 0.002411880396602612 0.9904373601301227 >1k 4.823760793205224E-4 1.0448553747054674 >5k 2.411880396602612E-4 0.8992982462884885 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6098 0.8978236096101578 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4159 0.6123398478794108 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2926 0.43080221084278814 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 897 0.13206752670060867 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 855 0.12588376290860692 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 702 0.10335719480917199 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 689 0.10144317268307622 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 1.4723247123813674E-4 0.0 0.0 0.0 0.0 9 1.4723247123813674E-4 0.0 0.0 0.0 0.0 10-11 2.944649424762735E-4 0.0 0.0 0.0 0.0 12-13 2.944649424762735E-4 0.0 0.0 0.0 0.0 14-15 2.944649424762735E-4 0.0 0.0 0.0 0.0 16-17 2.944649424762735E-4 0.0 0.0 1.4723247123813674E-4 0.0 18-19 2.944649424762735E-4 0.0 0.0 2.2084870685720512E-4 0.0 20-21 2.944649424762735E-4 0.0 0.0 3.680811780953419E-4 0.0 22-23 2.944649424762735E-4 0.0 0.0 7.361623561906838E-4 0.0 24-25 2.944649424762735E-4 0.0 0.0 0.0018404058904767091 0.0 26-27 2.944649424762735E-4 0.0 0.0 0.0028710331891436663 0.0 28-29 4.4169741371441023E-4 0.0 0.0 0.005079520257715717 0.0 30-31 4.4169741371441023E-4 0.0 0.0 0.00817140215371659 0.0 32-33 4.4169741371441023E-4 0.0 0.0 0.010527121693526778 0.0 34-35 4.4169741371441023E-4 0.0 0.0 0.013177306175813239 0.0 36-37 4.4169741371441023E-4 0.0 0.0 0.01626918807181411 0.0 38-39 4.4169741371441023E-4 0.0 0.0 0.020244464795243805 0.0 40-41 4.4169741371441023E-4 0.0 0.0 0.025250368817340454 0.0 42-43 4.4169741371441023E-4 0.0 0.0 0.03393708462039052 0.0 44-45 4.4169741371441023E-4 0.0 0.0 0.04843948303734699 0.0 46-47 4.4169741371441023E-4 0.0 0.0 0.061690405448779295 0.0 48-49 4.4169741371441023E-4 0.0 0.0 0.09930830185012324 0.0 50-51 4.4169741371441023E-4 0.0 0.0 0.13582195471718114 0.0 52-53 4.4169741371441023E-4 0.0 0.0 0.1736606998253823 0.0 54-55 4.4169741371441023E-4 0.0 0.0 0.25441771029950033 0.0 56-57 4.4169741371441023E-4 0.0 0.0 0.3222182633046623 0.0 58-59 4.4169741371441023E-4 0.0 0.0 0.39576088268811155 0.0 60-61 4.4169741371441023E-4 0.0 0.0 0.4918300701709958 0.0 62-63 4.4169741371441023E-4 0.0 0.0 0.622278039687985 0.0 64-65 5.88929884952547E-4 0.0 0.0 0.6995014708523877 0.0 66-67 7.361623561906837E-4 0.0 0.0 0.8395195509998558 0.0 68-69 7.361623561906837E-4 0.0 0.0 1.036001283867149 0.0 70-71 7.361623561906837E-4 0.0 0.0 1.138180618906416 0.0 72-73 8.097785918097521E-4 0.0 0.0 1.3164791415757997 0.0 74-75 8.833948274288205E-4 0.0 0.0 1.5464562616497692 0.0 76-77 8.833948274288205E-4 0.0 0.0 1.6874313528602851 0.0 78-79 8.833948274288205E-4 0.0 0.0 1.8160389164867978 0.0 80-81 0.0010306272986669572 0.0 0.0 2.040053121475623 0.0 82-83 0.0010306272986669572 0.0 0.0 2.3207518278911303 0.0 84-85 0.0011042435342860256 0.0 0.0 2.6525402018262714 0.0 86-87 0.001177859769905094 0.0 0.0 3.154971009926413 0.0 88-89 0.001177859769905094 0.0 0.0 3.6837564303781813 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3875 0.0 33.25551 1 GTATCAA 5980 0.0 32.76137 1 GTATAGG 225 0.0 29.58772 1 GTACATG 4640 0.0 27.567686 1 TACATGG 4615 0.0 27.304874 2 TATCAAC 7250 0.0 26.825714 2 ATCAACG 7340 0.0 26.367222 3 TCAACGC 7360 0.0 26.29557 4 CAACGCA 7445 0.0 25.995352 5 AACGCAG 7605 0.0 25.442814 6 GTCTAAT 150 1.0490439E-6 25.360905 1 ACATGGG 4745 0.0 25.254011 3 CATGGGA 3375 0.0 23.529284 4 GAGTACT 4115 0.0 23.09947 12-13 TAGACTG 270 4.0017767E-11 22.895262 5 ACGCAGA 8500 0.0 22.641958 7 CGCAGAG 8500 0.0 22.586052 8 TCTAGAC 190 3.2485877E-7 22.524488 3 ATGGGAG 1240 0.0 22.241922 5 GCAGAGT 8565 0.0 22.137238 9 >>END_MODULE