FastQCFastQC Report
Thu 26 May 2016
SRR936988_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936988_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679198
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64440.9487660446585531No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48090.7080409541841995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34270.5045656789330946No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12720.18727970341490993No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12340.18168486950786072No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10730.15798044163852074No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8430.12411697325374928No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA8300.12220295112765349No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8300.12220295112765349No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7230.10644907670517287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCCG1751.5097612E-1029.861178
GTGTAGG2850.028.3371541
GTATCAA82350.028.2096351
TACGCGA350.00839260427.1425280-81
GGCCCGC1955.329639E-1026.7984859
TGTAGGA4250.024.587932
GGTATCA57250.024.5622631
GACGAAT1102.1700544E-923.74970470-71
AGTACTT51500.023.70533612-13
TATCAAC99800.023.2261662
GAGTACT49950.023.20462412-13
ATCAACG100250.023.0728283
TCAACGC100600.023.0397684
CAACGCA102050.022.7124045
AACGCAG103500.022.4434036
GTACATG47850.022.4377171
GTATAGA1703.1421587E-622.3559571
TAGACAG3200.022.263715
CGGTTCA1507.2759576E-1222.16965534-35
GTAGGAG4750.021.9981063
Produced by FastQC (version 0.11.5)