##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936988_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 679198 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15288767045839 33.0 31.0 34.0 31.0 34.0 2 32.26518776557057 33.0 31.0 34.0 31.0 34.0 3 32.37607737360828 34.0 31.0 34.0 31.0 34.0 4 36.054947158266074 37.0 35.0 37.0 35.0 37.0 5 35.89815929964458 37.0 35.0 37.0 35.0 37.0 6 36.00847175639504 37.0 35.0 37.0 35.0 37.0 7 35.970058804649014 37.0 35.0 37.0 35.0 37.0 8 35.931336664713385 37.0 35.0 37.0 35.0 37.0 9 37.612326596957 39.0 37.0 39.0 35.0 39.0 10-11 37.5303343060492 39.0 37.0 39.0 35.0 39.0 12-13 37.42034207992367 39.0 37.0 39.0 34.0 39.0 14-15 38.55980288516751 40.0 38.0 41.0 34.0 41.0 16-17 38.56452242203305 40.0 38.0 41.0 34.0 41.0 18-19 38.73189187836242 40.0 38.0 41.0 34.5 41.0 20-21 38.755402695532084 40.0 38.0 41.0 34.5 41.0 22-23 38.660636515419654 40.0 38.0 41.0 34.0 41.0 24-25 38.54908877823551 40.0 38.0 41.0 34.0 41.0 26-27 38.327521576918656 40.0 38.0 41.0 34.0 41.0 28-29 38.157386358617075 40.0 38.0 41.0 34.0 41.0 30-31 38.0761294939031 40.0 37.0 41.0 34.0 41.0 32-33 37.882149976884506 40.0 37.0 41.0 33.0 41.0 34-35 37.71475622719737 40.0 36.5 41.0 33.0 41.0 36-37 37.52824360495761 40.0 36.0 41.0 33.0 41.0 38-39 37.32876716362533 40.0 36.0 41.0 33.0 41.0 40-41 36.89319093990265 39.5 35.5 41.0 31.5 41.0 42-43 36.65945350251326 39.0 35.0 41.0 31.0 41.0 44-45 36.33353307871931 39.0 35.0 41.0 30.5 41.0 46-47 35.7671599445228 39.0 35.0 40.5 29.0 41.0 48-49 35.24785482289406 39.0 35.0 40.0 26.5 41.0 50-51 34.92328452086137 39.0 35.0 40.0 25.5 41.0 52-53 34.638252762817324 38.0 34.5 40.0 24.0 41.0 54-55 34.26970927476229 38.0 34.0 40.0 23.0 41.0 56-57 34.03106016213239 38.0 34.0 40.0 22.5 41.0 58-59 33.7148784301485 37.5 33.0 40.0 22.0 41.0 60-61 33.3112649036069 37.0 33.0 40.0 20.5 41.0 62-63 32.91962505778875 36.5 32.5 39.0 19.0 40.5 64-65 32.59361776683677 36.0 32.0 39.0 18.0 40.0 66-67 32.268797169602976 35.5 32.0 39.0 15.5 40.0 68-69 31.818394636026607 35.0 31.0 38.0 12.0 40.0 70-71 31.365829993610113 35.0 31.0 38.0 8.0 40.0 72-73 30.951160044640886 35.0 31.0 37.0 7.0 39.0 74-75 30.455678609183185 34.0 30.0 36.5 4.5 39.0 76-77 29.03249567872697 32.5 28.5 35.0 2.0 37.0 78-79 29.683364055842333 34.0 29.5 35.0 2.0 37.5 80-81 29.55832614348099 34.0 29.5 35.0 2.0 37.0 82-83 29.228066778759654 34.0 29.0 35.0 2.0 37.0 84-85 28.797659150939786 34.0 29.0 35.0 2.0 36.0 86-87 28.415914063351188 34.0 29.0 35.0 2.0 36.0 88-89 28.034228604913444 33.5 28.5 35.0 2.0 35.0 90-91 27.582086519689398 33.0 27.0 35.0 2.0 35.0 92-93 27.102090995556523 33.0 26.0 35.0 2.0 35.0 94-95 26.57169485186941 32.5 25.0 34.0 2.0 35.0 96-97 25.819730034540736 32.0 24.0 34.0 2.0 35.0 98-99 25.00236160883866 32.0 19.5 34.0 2.0 35.0 100-101 22.046300931392615 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11494452351374917 1101 2 0.19516788136618857 1101 3 0.16539144004946138 1101 4 0.0533259386446332 1101 5 0.08290726484460009 1101 6 0.12798779298657337 1101 7 0.06450918220874513 1101 8 0.14623532566136532 1101 9 0.041549366061843784 1101 10-11 0.08206446020673042 1101 12-13 0.1808252071063805 1101 14-15 0.1506534153791037 1101 16-17 0.24710038491314634 1101 18-19 0.14710070710773238 1101 20-21 0.05888592134095916 1101 22-23 0.09939991983351604 1101 24-25 0.08639536558564487 1101 26-27 0.18304842129609966 1101 28-29 0.1277038631742542 1101 30-31 0.03735492611994573 1101 32-33 0.1292968686099698 1101 34-35 -0.05238492832751973 1101 36-37 0.10405407003376865 1101 38-39 -0.08122161014798479 1101 40-41 0.07673880796134114 1101 42-43 -0.0026188870802315023 1101 44-45 -0.15249628136036364 1101 46-47 -0.1289940976559052 1101 48-49 0.1141008212154162 1101 50-51 0.2887089469455333 1101 52-53 0.2225819325273406 1101 54-55 -0.09043944526619185 1101 56-57 0.026014411987276276 1101 58-59 0.1256697998730374 1101 60-61 0.16006279645728227 1101 62-63 0.2982642326113236 1101 64-65 0.19191591082546466 1101 66-67 0.29244768259236764 1101 68-69 0.023565078019224472 1101 70-71 0.22111311594034078 1101 72-73 0.3522711922743582 1101 74-75 0.23549723228672903 1101 76-77 5.441473881617753E-5 1101 78-79 0.0050767503283069 1101 80-81 -0.017552946267144875 1101 82-83 0.2181266260774528 1101 84-85 0.14213415501134818 1101 86-87 -0.23349403471907948 1101 88-89 -0.04117209815555256 1101 90-91 0.16208326768981962 1101 92-93 -0.10186597473260761 1101 94-95 -0.318315790895781 1101 96-97 -0.4201145910674917 1101 98-99 0.07126525546441087 1101 100-101 0.25938750790575327 1102 1 -0.2045982209939332 1102 2 -0.05566686441947155 1102 3 -0.052566830783980834 1102 4 -0.040880389502788717 1102 5 -0.1989319376117109 1102 6 -0.07007845599132168 1102 7 0.0444947779026208 1102 8 -0.020260041675520313 1102 9 -0.015590241989407616 1102 10-11 0.031239190938265438 1102 12-13 0.11469265499907522 1102 14-15 -0.020862069249986348 1102 16-17 0.02933110290824459 1102 18-19 0.03301427044064553 1102 20-21 0.10458006268727615 1102 22-23 0.06536270690429546 1102 24-25 -0.048138687601976926 1102 26-27 0.019793752080701665 1102 28-29 -0.17640894528637574 1102 30-31 0.013532315691449526 1102 32-33 -0.0738184687269694 1102 34-35 0.10473978805386253 1102 36-37 0.05143503143997208 1102 38-39 0.12697503570644386 1102 40-41 0.34815005090133155 1102 42-43 0.25526395067637253 1102 44-45 0.3632366324791434 1102 46-47 0.33519387312393434 1102 48-49 0.328971642748229 1102 50-51 0.40681721750821964 1102 52-53 0.3784776800079399 1102 54-55 0.46444854230062305 1102 56-57 0.4157789611487459 1102 58-59 0.23596617117922136 1102 60-61 0.39948619099988747 1102 62-63 0.2628789174223627 1102 64-65 0.3906625737376217 1102 66-67 0.45584693638432583 1102 68-69 0.25259598229557056 1102 70-71 0.1780491142508147 1102 72-73 0.2730732305378254 1102 74-75 0.22094211415517506 1102 76-77 0.324836590222251 1102 78-79 0.031167497456920046 1102 80-81 0.3231646697660402 1102 82-83 0.37485251646392825 1102 84-85 0.22688833415543286 1102 86-87 0.03921955244051034 1102 88-89 0.2386986963014941 1102 90-91 0.18240796024452877 1102 92-93 0.1648116124919632 1102 94-95 -0.022054953821477596 1102 96-97 0.24188186742265927 1102 98-99 0.06275967514957514 1102 100-101 -0.24203721876441975 1103 1 0.06349250149284558 1103 2 0.2384185345139187 1103 3 0.18437507649481688 1103 4 0.1571577002362048 1103 5 -0.17686171619884306 1103 6 -0.07205749806896478 1103 7 0.03947579013917846 1103 8 0.11649880896993636 1103 9 0.20017293285346938 1103 10-11 0.0720811245492996 1103 12-13 0.07988492791525914 1103 14-15 0.20656914174737295 1103 16-17 0.26685961627899246 1103 18-19 0.12731381708201894 1103 20-21 0.0819111794596381 1103 22-23 0.02770665667635086 1103 24-25 0.17867387446119665 1103 26-27 0.10347474496465026 1103 28-29 0.03487045243509357 1103 30-31 0.23991340978001574 1103 32-33 0.23595269461856105 1103 34-35 0.3386033616873618 1103 36-37 0.28618692149178315 1103 38-39 0.3715088758031442 1103 40-41 0.48108882369219685 1103 42-43 0.2118195672155636 1103 44-45 0.4309663177930503 1103 46-47 0.42275871229107054 1103 48-49 0.7225633915577276 1103 50-51 0.6763854005521637 1103 52-53 0.6536473325810448 1103 54-55 0.8909514626408495 1103 56-57 0.6277928061946341 1103 58-59 0.7088474572075967 1103 60-61 0.5810996274202296 1103 62-63 0.5945207482424948 1103 64-65 0.629004848398715 1103 66-67 0.7532844607733722 1103 68-69 0.6857920495918854 1103 70-71 0.6700114369444421 1103 72-73 0.6315882192911033 1103 74-75 0.5199633798157173 1103 76-77 0.6630846398918315 1103 78-79 0.7866983775564123 1103 80-81 0.8932490281001897 1103 82-83 1.0949332263556535 1103 84-85 0.7281963800813891 1103 86-87 0.48117346785375403 1103 88-89 0.7984186228250358 1103 90-91 0.532462686881015 1103 92-93 0.33455396900506784 1103 94-95 0.678136985976046 1103 96-97 0.57019853953555 1103 98-99 0.3747516295408708 1103 100-101 0.8233314484119738 1104 1 0.028583012187304746 1104 2 0.0801329776303703 1104 3 0.013133853666701611 1104 4 0.01301132688892892 1104 5 -0.02194074187051598 1104 6 0.12812193595907218 1104 7 0.07625665341449661 1104 8 0.11474493082945258 1104 9 0.10418350903434259 1104 10-11 0.17157169064576294 1104 12-13 0.2536449189513377 1104 14-15 0.18944198480406982 1104 16-17 0.008737619814759512 1104 18-19 0.09630036497674155 1104 20-21 0.04991029551750614 1104 22-23 0.08798979828139153 1104 24-25 0.11736711852882564 1104 26-27 0.07047125588320569 1104 28-29 0.09268657068413688 1104 30-31 0.22481441759855159 1104 32-33 0.08858646867796693 1104 34-35 0.2244071420962186 1104 36-37 0.18208211521206152 1104 38-39 0.08412886161026734 1104 40-41 0.4598617626130377 1104 42-43 0.34919164460406904 1104 44-45 0.5564798757223883 1104 46-47 0.29223874266880046 1104 48-49 0.28401215403874147 1104 50-51 0.3442554268214266 1104 52-53 0.4190416205718748 1104 54-55 0.458510786362865 1104 56-57 0.3489324068021915 1104 58-59 0.19234084942890206 1104 60-61 0.08489956703827062 1104 62-63 0.08765113281957326 1104 64-65 0.07080472887977152 1104 66-67 0.04167602846342078 1104 68-69 -0.09185565278106722 1104 70-71 0.015287326489026754 1104 72-73 0.15153021836981395 1104 74-75 0.006019114857174657 1104 76-77 0.11799464275530624 1104 78-79 0.03760177264119591 1104 80-81 0.10386769421906195 1104 82-83 0.01514772238413542 1104 84-85 0.17067672481882212 1104 86-87 0.016055723026685342 1104 88-89 0.05327252337532329 1104 90-91 -0.12189142842985845 1104 92-93 -0.37353736960701767 1104 94-95 -0.1082664900330137 1104 96-97 -0.02117936751357874 1104 98-99 -0.280056517666619 1104 100-101 0.6669483902211901 1105 1 -0.09149274538845731 1105 2 0.10229206853946238 1105 3 0.05006567184852173 1105 4 0.07892041779801673 1105 5 -0.05243316611294091 1105 6 -0.046688670101531216 1105 7 0.1260672594751 1105 8 0.05186614295066505 1105 9 0.06460017570100973 1105 10-11 -0.02568209723302317 1105 12-13 -0.013968717412296883 1105 14-15 0.17353289389497917 1105 16-17 -0.058686622609478434 1105 18-19 -0.05786630168991991 1105 20-21 0.12756181066902172 1105 22-23 0.24234585888744675 1105 24-25 -0.03613666934995763 1105 26-27 -0.05897950169255495 1105 28-29 0.03501611613868505 1105 30-31 0.04081820547733628 1105 32-33 0.20241222625372757 1105 34-35 0.026585172399244072 1105 36-37 0.11380560006054452 1105 38-39 0.15628795251936367 1105 40-41 0.21061933837061986 1105 42-43 -0.007626537214115103 1105 44-45 -0.22817921518669948 1105 46-47 -0.19033701490695876 1105 48-49 -0.2902302702036863 1105 50-51 -0.10508169439069093 1105 52-53 0.11354919632945837 1105 54-55 0.33218502878710865 1105 56-57 0.332076346196132 1105 58-59 0.16629918276223776 1105 60-61 -0.0394375541738512 1105 62-63 -0.22380720051376102 1105 64-65 0.15205472887977578 1105 66-67 0.07898663452402133 1105 68-69 0.11827692297650927 1105 70-71 0.2762721749738759 1105 72-73 0.36980673352132953 1105 74-75 0.2358486603117207 1105 76-77 0.3281272185128792 1105 78-79 0.39555631809574265 1105 80-81 0.24439799724936861 1105 82-83 0.28948484359625937 1105 84-85 0.046528997546094075 1105 86-87 0.04048754120850262 1105 88-89 0.4212649476177468 1105 90-91 0.7240366018731699 1105 92-93 0.6310080849384363 1105 94-95 0.675635025118499 1105 96-97 0.7628168446076344 1105 98-99 0.5962692399091374 1105 100-101 0.7749029356757369 1106 1 -0.12904792541328902 1106 2 0.004868285948759876 1106 3 0.04454766936541432 1106 4 -0.022985017434429267 1106 5 -0.061065391247446144 1106 6 0.01883243625796638 1106 7 -0.043804446967165234 1106 8 0.08399643678429669 1106 9 -0.07443451873960072 1106 10-11 0.060559970638408345 1106 12-13 -0.00796013002093332 1106 14-15 0.12879137782379502 1106 16-17 0.006820688743808034 1106 18-19 0.015718847985887408 1106 20-21 -0.14128561656232108 1106 22-23 -0.04695266504688789 1106 24-25 -0.10962180523077336 1106 26-27 0.20083909397581579 1106 28-29 0.14528649826035434 1106 30-31 -0.0634358295619819 1106 32-33 0.08914401684553042 1106 34-35 0.03550536828833373 1106 36-37 0.17475279967428747 1106 38-39 0.06474256694893654 1106 40-41 0.12728715462113627 1106 42-43 0.4052683448385821 1106 44-45 -0.20490100439900516 1106 46-47 -0.20139543675824711 1106 48-49 -0.1530380712796955 1106 50-51 -0.12763652954036786 1106 52-53 0.029631587004033122 1106 54-55 0.23318085579773395 1106 56-57 0.07192580819088334 1106 58-59 0.03252642100199665 1106 60-61 -0.025050224887060324 1106 62-63 0.056792709818701326 1106 64-65 -0.191731483011516 1106 66-67 -0.08768807922959354 1106 68-69 -0.20752318738353992 1106 70-71 -0.029126845580684346 1106 72-73 0.05685408488013621 1106 74-75 -0.17734884968966114 1106 76-77 -0.1646959295075341 1106 78-79 -0.26975527660697907 1106 80-81 -0.4122710260641931 1106 82-83 -0.25817517847574933 1106 84-85 -0.21740482091162505 1106 86-87 -0.5193845101089245 1106 88-89 -0.03747849975430384 1106 90-91 -0.19680765250674526 1106 92-93 0.019090061762828014 1106 94-95 0.13198503891350555 1106 96-97 0.3273852333509062 1106 98-99 0.29587349566855536 1106 100-101 0.6987458795300618 1107 1 0.1905740159751801 1107 2 0.23620542081219043 1107 3 0.12978868321215487 1107 4 0.12327411097983543 1107 5 0.05807819752342169 1107 6 0.08544911398937671 1107 7 0.04222492992964533 1107 8 -0.032153743412969504 1107 9 0.19237006206464713 1107 10-11 1.701754942473599E-4 1107 12-13 0.10352062917861105 1107 14-15 0.2949107348040698 1107 16-17 0.20168269557233742 1107 18-19 0.239559124446437 1107 20-21 0.29215698112356847 1107 22-23 0.13909656911472013 1107 24-25 0.16400537610458343 1107 26-27 0.36607376535290115 1107 28-29 0.2652717979568635 1107 30-31 0.22371948388642693 1107 32-33 0.19674816375372473 1107 34-35 0.13450420649015626 1107 36-37 0.1380776171059992 1107 38-39 0.04719704342845432 1107 40-41 0.06207530427970909 1107 42-43 -0.04285380994138421 1107 44-45 -0.32437949927761167 1107 46-47 -0.31006357740695734 1107 48-49 -0.4371940486127741 1107 50-51 -0.5519922057543312 1107 52-53 -0.5095509457160006 1107 54-55 -0.41136965871289277 1107 56-57 -0.7287191083493241 1107 58-59 -0.7731539422377622 1107 60-61 -0.711117241673854 1107 62-63 -0.746569984604669 1107 64-65 -0.35240194725658824 1107 66-67 -0.6106973143396104 1107 68-69 -0.8034737872507627 1107 70-71 -0.6103045295715788 1107 72-73 -0.5213509369332172 1107 74-75 -0.29121099877918866 1107 76-77 -0.37114479853257265 1107 78-79 -0.26468871031335084 1107 80-81 -0.4013406391142702 1107 82-83 -0.28107623594919673 1107 84-85 -0.3472565138175412 1107 86-87 -0.028244703109681524 1107 88-89 -0.1105532342004345 1107 90-91 -0.017724761763194152 1107 92-93 0.32248535766570896 1107 94-95 0.6558196842094084 1107 96-97 0.23763928778945242 1107 98-99 0.16643969445459206 1107 100-101 0.6850591856757369 1108 1 0.04839653200082239 1108 2 0.08844757969497152 1108 3 -0.024193402410553233 1108 4 0.05236780374540473 1108 5 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2316 96-97 -1.7611765340609402 2316 98-99 -1.918329006332808 2316 100-101 -2.218699426528989 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 6.0 5 12.0 6 43.0 7 100.0 8 210.0 9 482.0 10 800.0 11 1387.0 12 2155.0 13 3084.0 14 3941.0 15 5526.0 16 7718.0 17 11258.0 18 9575.0 19 5208.0 20 4177.0 21 4077.0 22 4263.0 23 4676.0 24 5215.0 25 6186.0 26 7446.0 27 9113.0 28 11048.0 29 13559.0 30 16754.0 31 21026.0 32 27031.0 33 35082.0 34 48159.0 35 68913.0 36 103718.0 37 145715.0 38 87424.0 39 4109.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.355202120609675 19.938737942714084 15.459980855607098 20.24607908106914 2 15.629302107121454 22.05152442750227 39.92620436913717 22.392969096239103 3 19.259213453534098 27.41649330017653 30.74601330689034 22.578279939399025 4 12.720002120147585 17.9230504212321 37.93253219237984 31.42441526624048 5 12.882095105442861 39.1379053951586 33.165435530630184 14.814563968768358 6 29.70577062947771 37.78618311596913 17.490775885676932 15.017270368876234 7 26.87357828016991 35.33197388084424 20.686989745213083 17.107458093772777 8 26.9581182512316 33.88820343993946 19.699851884133935 19.45382642469501 9 27.884251549992122 16.656433994849802 20.495379102086726 34.96393535307135 10-11 24.66821162606486 27.82104776515832 26.678891869528478 20.83184873924835 12-13 26.763427433842025 25.60842369377382 27.551124081916328 20.07702479046783 14-15 23.477466414592058 25.840224157935342 27.683519738973207 22.99878968849939 16-17 20.884852428894078 29.51753391499975 27.877732266585003 21.71988138952117 18-19 20.910253380654023 26.91618245409459 32.1161580134144 20.05740615183699 20-21 22.28805991794704 25.792205631515518 32.134709373074465 19.78502507746297 22-23 21.63056713394102 26.054017523892536 32.1319459883655 20.18346935380094 24-25 21.604146667530433 26.135092079458655 32.3623814038952 19.89837984911572 26-27 21.449431130120473 26.247888132685997 32.29288977064845 20.009790966545076 28-29 20.87956743068106 26.43428457849153 32.33897356807115 20.347174422756268 30-31 21.028993018236214 26.439271022588407 32.264450130889664 20.267285828285715 32-33 20.99227397038417 26.47131357226727 32.06217631837573 20.47423613897283 34-35 21.370793200215548 26.171970471055566 32.08696138681209 20.37027494191679 36-37 21.63759867546841 26.05658117430709 31.867455555089307 20.438364595135187 38-39 21.412279888035364 26.336647804659698 31.957628952959833 20.293443354345108 40-41 21.248032603206436 26.412734449651577 31.702142382843267 20.637090564298724 42-43 21.222530101678743 26.781292053274598 31.210412869295844 20.785764975750812 44-45 21.54821972546831 26.94167931648294 30.768426647506676 20.74167431054207 46-47 21.90821487201761 27.168927922025336 30.0358512651006 20.887005940856454 48-49 22.034222715614593 27.47976289682832 29.372730779537044 21.113283608020048 50-51 22.08516151830148 27.71916134161783 29.144924762213257 21.05075237786743 52-53 22.105376295008277 28.001478221628705 28.99103276012983 20.90211272323318 54-55 21.640596703759435 27.8132444441827 29.038439453590854 21.507719398467014 56-57 22.088862223432802 28.21197074488643 28.55185715495125 21.147309876729523 58-59 22.21827966092337 27.63658582371107 28.762178894945873 21.382955620419686 60-61 22.04850950364885 27.48383544136993 29.405030871491117 21.062624183490104 62-63 21.96958766074105 27.480425442948892 29.587837420015962 20.9621494762941 64-65 21.908207848232657 27.672952270878497 29.277419307344328 21.141420573544515 66-67 21.749622348711277 27.898123963851486 28.963277277023785 21.388976410413456 68-69 21.89519388336521 27.849872717341214 29.024642334992645 21.230291064300932 70-71 21.920589523588674 27.92610342428693 28.819664528097704 21.333642524026693 72-73 21.91165871976478 27.83211902905717 28.5291937299349 21.727028521243145 74-75 21.929810353966666 28.293112079627775 28.30989752046646 21.467180045939102 76-77 21.946904869875556 28.10087220772796 28.46394855093375 21.48827437146273 78-79 21.885409849461386 27.95922228463275 28.332679372403636 21.82268849350223 80-81 21.843159946759013 28.478847898041153 28.16766199041191 21.51033016478792 82-83 21.829077069177483 28.374619954504965 28.164518290034522 21.631784686283027 84-85 21.78877553950161 28.148758371449794 27.95830489782727 22.104161191221326 86-87 21.862719837454634 27.90251695021312 28.08383453941799 22.150928672914258 88-89 22.019825742545102 27.716726727942664 28.03277541990746 22.230672109604775 90-91 21.817290370836073 27.82695052795166 28.31054732792339 22.045211773288877 92-93 21.707955559350882 28.006118981504656 28.0642021914081 22.22172326773636 94-95 21.552772534665884 28.224538352586432 27.813391676653936 22.409297436093745 96-97 21.786379463924206 28.022880027090896 27.75410596367759 22.436634545307314 98-99 21.661185869333963 28.25669419212164 27.32175629923608 22.760363639308324 100-101 21.460690039002973 28.382944702353875 27.35987125632565 22.796494002317505 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 643.0 1 473.0 2 325.5 3 349.0 4 316.5 5 317.5 6 518.0 7 1066.0 8 1809.5 9 2142.0 10 1901.5 11 1565.0 12 1538.0 13 1675.5 14 1770.0 15 1852.0 16 1944.0 17 2052.0 18 2090.0 19 2149.5 20 2271.0 21 2464.0 22 2782.5 23 3150.0 24 3553.5 25 4195.0 26 5032.0 27 5876.0 28 6701.5 29 7668.5 30 8864.0 31 10182.5 32 11581.5 33 12947.5 34 14445.5 35 15569.5 36 17044.5 37 18789.0 38 19363.5 39 19410.5 40 19507.0 41 20247.0 42 20982.5 43 21450.0 44 22784.5 45 24059.0 46 24052.0 47 24481.5 48 28425.0 49 28116.5 50 23626.0 51 21952.0 52 20863.5 53 20402.0 54 19864.0 55 18342.0 56 16322.0 57 14626.0 58 13637.0 59 12539.0 60 10821.0 61 8806.0 62 7019.5 63 5309.5 64 3756.5 65 2780.0 66 1955.5 67 1440.0 68 1024.0 69 597.5 70 402.0 71 285.5 72 193.5 73 150.5 74 118.0 75 86.0 76 57.0 77 28.5 78 11.5 79 4.0 80 2.0 81 2.0 82 0.5 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02179040574324424 2 0.0036808117809534187 3 1.4723247123813674E-4 4 0.0 5 0.002208487068572051 6 0.0 7 4.4169741371441023E-4 8 0.0 9 1.4723247123813674E-4 10-11 0.0 12-13 0.009717343101717025 14-15 0.005005904022096649 16-17 0.0 18-19 3.6808117809534183E-4 20-21 3.6808117809534183E-4 22-23 0.0016195571836195041 24-25 2.944649424762735E-4 26-27 7.361623561906837E-5 28-29 7.361623561906837E-5 30-31 0.0 32-33 7.361623561906837E-5 34-35 0.0 36-37 0.0021348708329529827 38-39 0.008097785918097521 40-41 1.4723247123813674E-4 42-43 0.0 44-45 7.361623561906837E-4 46-47 4.4169741371441023E-4 48-49 0.0 50-51 0.0026501844822864615 52-53 5.153136493334787E-4 54-55 0.0 56-57 7.361623561906837E-5 58-59 7.361623561906837E-5 60-61 0.0016931734192385725 62-63 0.0 64-65 7.361623561906837E-5 66-67 0.0 68-69 1.4723247123813674E-4 70-71 8.097785918097521E-4 72-73 3.6808117809534183E-4 74-75 0.005594833907049196 76-77 2.944649424762735E-4 78-79 5.153136493334787E-4 80-81 0.0032391143672390083 82-83 4.4169741371441023E-4 84-85 0.004343357901525034 86-87 4.4169741371441023E-4 88-89 0.004490590372763171 90-91 0.0024293357754292563 92-93 0.0 94-95 0.0 96-97 4.4169741371441023E-4 98-99 0.0012514760055241624 100-101 0.002797416953524598 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 679198.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.979042489022845 #Duplication Level Percentage of deduplicated Percentage of total 1 80.1166407043405 47.252027561790754 2 11.613251712220524 13.698769323415434 3 3.466159941015844 6.1329238330476725 4 1.487289283935204 3.5087559148273115 5 0.8482588213065001 2.5014746531762255 6 0.49653997488469037 1.7571271365733494 7 0.3471458716305402 1.4332031778950551 8 0.2397296128743901 1.131121841887652 9 0.1832769660994179 0.9728549973753091 >10 1.0919892297061597 11.874883904463744 >50 0.0736463232207897 2.935467055573963 >100 0.033566589406834664 3.6271817842816385 >500 0.00100198774345963 0.4766114796100591 >1k 0.0012524846793245373 1.7455563024156382 >5k 2.504969358649075E-4 0.9520410336662186 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6444 0.9487660446585531 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4809 0.7080409541841995 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3427 0.5045656789330946 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1272 0.18727970341490993 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1234 0.18168486950786072 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1073 0.15798044163852074 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 843 0.12411697325374928 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 830 0.12220295112765349 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 830 0.12220295112765349 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 723 0.10644907670517287 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 1.4723247123813674E-4 0.0 0.0 0.0 0.0 14-15 1.4723247123813674E-4 0.0 0.0 0.0 0.0 16-17 2.2084870685720512E-4 0.0 0.0 1.4723247123813674E-4 0.0 18-19 2.944649424762735E-4 0.0 0.0 2.2084870685720512E-4 0.0 20-21 2.944649424762735E-4 0.0 0.0 3.680811780953419E-4 0.0 22-23 2.944649424762735E-4 0.0 0.0 6.625461205716154E-4 0.0 24-25 2.944649424762735E-4 0.0 0.0 0.0016931734192385725 0.0 26-27 2.944649424762735E-4 0.0 0.0 0.0027238007179055295 0.0 28-29 2.944649424762735E-4 0.0 0.0 0.004785055315239445 0.0 30-31 2.944649424762735E-4 0.0 0.0 0.007876937211240316 0.0 32-33 2.944649424762735E-4 0.0 0.0 0.010306272986669572 0.0 34-35 2.944649424762735E-4 0.0 0.0 0.013030073704575103 0.0 36-37 2.944649424762735E-4 0.0 0.0 0.016121955600575975 0.0 38-39 2.944649424762735E-4 0.0 0.0 0.020244464795243805 0.0 40-41 2.944649424762735E-4 0.0 0.0 0.025250368817340454 0.0 42-43 3.680811780953419E-4 0.0 0.0 0.03415793332724772 0.0 44-45 5.88929884952547E-4 0.0 0.0 0.04866033174420419 0.0 46-47 6.625461205716154E-4 0.0 0.0 0.062205719098112774 0.0 48-49 7.361623561906837E-4 0.0 0.0 0.09960276679259951 0.0 50-51 7.361623561906837E-4 0.0 0.0 0.13589557095280022 0.0 52-53 7.361623561906837E-4 0.0 0.0 0.1731453861760488 0.0 54-55 7.361623561906837E-4 0.0 0.0 0.2536815479433096 0.0 56-57 8.833948274288205E-4 0.0 0.0 0.32155571718409065 0.0 58-59 8.833948274288205E-4 0.0 0.0 0.39495110409630185 0.0 60-61 8.833948274288205E-4 0.0 0.0 0.4916092214641386 0.0 62-63 8.833948274288205E-4 0.0 0.0 0.6216891098030324 0.0 64-65 8.833948274288205E-4 0.0 0.0 0.6997223195592449 0.0 66-67 8.833948274288205E-4 0.0 0.0 0.8390778535861413 0.0 68-69 8.833948274288205E-4 0.0 0.0 1.0362221325740064 0.0 70-71 0.0010306272986669572 0.0 0.0 1.1389167812626066 0.0 72-73 0.001177859769905094 0.0 0.0 1.3183195474662763 0.0 74-75 0.001177859769905094 0.0 0.0 1.5488855974251985 0.0 76-77 0.001177859769905094 0.0 0.0 1.6896398399288572 0.0 78-79 0.001177859769905094 0.0 0.0 1.8181001710841316 0.0 80-81 0.0014723247123813673 0.0 0.0 2.042703305957909 0.0 82-83 0.0016195571836195041 0.0 0.0 2.3242117909652267 0.0 84-85 0.001766789654857641 0.0 0.0 2.6560001649003677 0.0 86-87 0.001766789654857641 0.0 0.0 3.159093519121081 0.0 88-89 0.001766789654857641 0.0 0.0 3.688909566871516 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGCCCG 175 1.5097612E-10 29.86117 8 GTGTAGG 285 0.0 28.337154 1 GTATCAA 8235 0.0 28.209635 1 TACGCGA 35 0.008392604 27.14252 80-81 GGCCCGC 195 5.329639E-10 26.798485 9 TGTAGGA 425 0.0 24.58793 2 GGTATCA 5725 0.0 24.562263 1 GACGAAT 110 2.1700544E-9 23.749704 70-71 AGTACTT 5150 0.0 23.705336 12-13 TATCAAC 9980 0.0 23.226166 2 GAGTACT 4995 0.0 23.204624 12-13 ATCAACG 10025 0.0 23.072828 3 TCAACGC 10060 0.0 23.039768 4 CAACGCA 10205 0.0 22.712404 5 AACGCAG 10350 0.0 22.443403 6 GTACATG 4785 0.0 22.437717 1 GTATAGA 170 3.1421587E-6 22.355957 1 TAGACAG 320 0.0 22.26371 5 CGGTTCA 150 7.2759576E-12 22.169655 34-35 GTAGGAG 475 0.0 21.998106 3 >>END_MODULE