FastQCFastQC Report
Thu 26 May 2016
SRR936987_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936987_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121766
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61820.29136106432094777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46650.21986401893517005No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29930.1410617381935614No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28050.13220119466519872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA108950.029.884091
TCAACGC127900.025.268784
CAACGCA127600.025.2519075
ATCAACG128050.025.2385853
GGTATCA76250.025.183621
TATCAAC128250.025.128672
AACGCAG131300.024.6127056
GTACATG83950.023.326691
TACATGG83750.023.2132322
ACGCAGA146300.021.9262497
CGCAGAG145050.021.854138
GAGTACT64150.021.73873912-13
TATACCG1553.8930797E-521.4625595
ACATGGG86750.021.4211583
AGAGTAC110250.020.34357810-11
AGTACTT69050.020.29930912-13
GTACTTT67900.020.22277514-15
GCAGAGT152800.020.1552099
ATACCGT2101.9484953E-518.1044716
CATGGGA48450.017.8535064
Produced by FastQC (version 0.11.5)