FastQCFastQC Report
Thu 26 May 2016
SRR936987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936987_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121766
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62290.29357620020303843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51390.2422038999588079No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34580.16297744426105423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31540.14864975685348902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA120800.026.9416261
GGTATCA88500.026.3058911
TCAACGC141100.022.8907094
ATCAACG142050.022.7721313
TATCAAC144000.022.5307942
CAACGCA143950.022.4705035
AACGCAG147850.021.8772586
GTACATG84950.021.644511
GAGTACT73300.021.5778112-13
TACATGG85000.020.7894152
GTACTTT75900.020.65115714-15
AGAGTAC117750.020.63249810-11
ACGCAGA163050.019.7795357
ACATGGG84950.019.6823583
CGCAGAG163650.019.5328758
AGTACTT77150.019.51598212-13
GCAGAGT173250.018.121559
ACTTTTT93250.017.01276816-17
TACTTTT88050.016.74960114-15
GTGTACG3001.4636789E-615.837221
Produced by FastQC (version 0.11.5)