##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936987_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2121766 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17335087846634 33.0 31.0 34.0 31.0 34.0 2 32.17132520739799 33.0 31.0 34.0 30.0 34.0 3 32.35565467634037 34.0 31.0 34.0 31.0 34.0 4 36.07766077880407 37.0 35.0 37.0 35.0 37.0 5 36.00277316160218 37.0 35.0 37.0 35.0 37.0 6 36.041606850142756 37.0 35.0 37.0 35.0 37.0 7 35.983281851061804 37.0 35.0 37.0 35.0 37.0 8 35.959559159681135 37.0 35.0 37.0 35.0 37.0 9 37.655472846675835 39.0 37.0 39.0 35.0 39.0 10-11 37.58450531302698 39.0 37.0 39.0 35.0 39.0 12-13 37.49674257198956 39.0 37.0 39.0 35.0 39.0 14-15 38.72816441586866 40.0 38.0 41.0 34.5 41.0 16-17 38.641298804863496 40.0 38.0 41.0 34.0 41.0 18-19 38.68860491684757 40.0 38.0 41.0 34.5 41.0 20-21 38.76121188670193 40.0 38.0 41.0 34.5 41.0 22-23 38.69203979138133 40.0 38.0 41.0 34.0 41.0 24-25 38.60456855279988 40.0 38.0 41.0 34.0 41.0 26-27 38.457065717897265 40.0 38.0 41.0 34.0 41.0 28-29 38.285721658279 40.0 38.0 41.0 34.0 41.0 30-31 38.183489602529214 40.0 38.0 41.0 34.0 41.0 32-33 38.199935572537214 40.0 38.0 41.0 34.0 41.0 34-35 38.129650724914995 40.0 38.0 41.0 34.0 41.0 36-37 37.9918751643678 40.0 37.5 41.0 33.0 41.0 38-39 37.84874415934651 40.0 37.0 41.0 33.0 41.0 40-41 37.69009070745784 40.0 37.0 41.0 33.0 41.0 42-43 37.4899772170918 40.0 37.0 41.0 33.0 41.0 44-45 37.18424557656216 40.0 37.0 41.0 31.5 41.0 46-47 36.94885981771788 40.0 36.0 41.0 31.0 41.0 48-49 36.65972237277815 39.0 36.0 41.0 31.0 41.0 50-51 36.412641403434684 39.0 36.0 41.0 30.0 41.0 52-53 36.17605499381176 39.0 35.0 40.0 30.0 41.0 54-55 35.94362408484253 39.0 35.0 40.0 29.0 41.0 56-57 35.6680234766699 38.5 35.0 40.0 28.5 41.0 58-59 35.35507355665045 38.0 34.5 40.0 28.0 41.0 60-61 35.0233765174859 38.0 34.0 40.0 27.5 41.0 62-63 34.66419506203795 37.5 34.0 40.0 27.0 41.0 64-65 34.273836982966074 37.0 33.5 39.5 26.0 41.0 66-67 33.89252113569545 36.0 33.0 39.0 26.0 40.0 68-69 33.456064900653516 36.0 33.0 39.0 26.0 40.0 70-71 32.985214674945304 35.0 32.0 38.0 25.0 40.0 72-73 32.64676241395139 35.0 32.0 37.5 24.5 39.5 74-75 32.205545050679476 35.0 31.5 37.0 23.0 39.0 76-77 30.791186445630668 33.5 29.5 35.5 21.5 38.0 78-79 31.524714318167035 34.0 31.0 36.0 23.0 38.5 80-81 31.36701054687463 34.0 31.0 36.0 22.0 37.5 82-83 31.036460429660952 34.0 31.0 35.0 20.5 37.0 84-85 30.6665009242301 34.0 31.0 35.0 20.0 36.5 86-87 30.32148078534579 34.0 30.5 35.0 18.0 36.0 88-89 29.97615712571509 34.0 30.0 35.0 17.0 36.0 90-91 29.564533506522395 34.0 30.0 35.0 9.0 35.0 92-93 29.128222197923805 34.0 29.0 35.0 4.5 35.0 94-95 28.67173053013386 34.0 29.0 35.0 2.0 35.0 96-97 28.064454562850003 33.5 29.0 35.0 2.0 35.0 98-99 27.358626257560918 33.0 27.0 35.0 2.0 35.0 100-101 24.38403952179458 30.5 21.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32625963401458336 1101 2 0.16096707991265902 1101 3 0.1732820489521174 1101 4 0.1922959086208138 1101 5 0.2343691186135004 1101 6 0.20813702992192162 1101 7 0.24461621592348592 1101 8 0.26726630570203014 1101 9 0.34532307216771585 1101 10-11 0.37424880989029674 1101 12-13 0.3765945445390386 1101 14-15 0.3786620109577328 1101 16-17 0.4148587245728734 1101 18-19 0.3934055151791327 1101 20-21 0.4189139412572658 1101 22-23 0.3303195033052546 1101 24-25 0.30198217136297245 1101 26-27 0.3294521400631325 1101 28-29 0.32661982496978936 1101 30-31 0.2022204546844577 1101 32-33 0.3729898568005794 1101 34-35 0.33863325963900337 1101 36-37 0.34523264548752763 1101 38-39 0.3671855278428069 1101 40-41 0.3992978005787222 1101 42-43 0.38185319467353906 1101 44-45 0.4858219418232679 1101 46-47 0.48082591742107894 1101 48-49 0.391706061066202 1101 50-51 0.377725147704723 1101 52-53 0.2745483319240165 1101 54-55 0.40142046560730194 1101 56-57 0.44878948296869936 1101 58-59 0.46216152108721076 1101 60-61 0.3796434744998649 1101 62-63 0.41345362692862153 1101 64-65 0.3353425220150612 1101 66-67 0.41374901739298053 1101 68-69 0.2621428713720704 1101 70-71 0.40741503007275526 1101 72-73 0.47326724379430374 1101 74-75 0.4447879723645656 1101 76-77 0.2883036926783227 1101 78-79 0.4425066219046663 1101 80-81 0.6240800666383528 1101 82-83 0.6385142348528596 1101 84-85 0.6440562967987731 1101 86-87 0.4765607161732248 1101 88-89 0.6185275806839066 1101 90-91 0.7928590319659392 1101 92-93 0.6235491903793253 1101 94-95 0.5458903380184701 1101 96-97 0.6177249934273483 1101 98-99 0.6687035917616697 1101 100-101 0.7908086566940469 1102 1 0.17818603498953678 1102 2 -0.010308362039076258 1102 3 0.10985726057872824 1102 4 0.11142119820627983 1102 5 0.19018055932294686 1102 6 0.17250043848964225 1102 7 0.1748891848870855 1102 8 0.18675915487703065 1102 9 0.23470656320636607 1102 10-11 0.21828092230573048 1102 12-13 0.2568198793266063 1102 14-15 0.3208404711109907 1102 16-17 0.3255575172230607 1102 18-19 0.23469985816025485 1102 20-21 0.3253062217732037 1102 22-23 0.289507363499915 1102 24-25 0.2732057764224294 1102 26-27 0.305451475769388 1102 28-29 0.2558487293186147 1102 30-31 0.19343901648443307 1102 32-33 0.3903262139187049 1102 34-35 0.2607769746244273 1102 36-37 0.3103941630975129 1102 38-39 0.4502755683415174 1102 40-41 0.44502813598803925 1102 42-43 0.4998485743314518 1102 44-45 0.6078943632736937 1102 46-47 0.5185424972282178 1102 48-49 0.4240654496798797 1102 50-51 0.4790993443295619 1102 52-53 0.6360030945173634 1102 54-55 0.5406551316460408 1102 56-57 0.6495445879460462 1102 58-59 0.5131668250575672 1102 60-61 0.5176686130521304 1102 62-63 0.6012936729732914 1102 64-65 0.5407707828700268 1102 66-67 0.53120262317087 1102 68-69 0.42796414419877493 1102 70-71 0.3804289108405783 1102 72-73 0.4620542340572271 1102 74-75 0.41213561474251037 1102 76-77 0.5180349488183076 1102 78-79 0.6708565785240843 1102 80-81 0.5620478732214131 1102 82-83 0.5432371313526794 1102 84-85 0.5734562608568972 1102 86-87 0.4515912901706436 1102 88-89 0.5123403072413453 1102 90-91 0.6038974814261948 1102 92-93 0.6992756242840308 1102 94-95 0.7617082629186811 1102 96-97 0.9962290462500967 1102 98-99 1.004680633737344 1102 100-101 1.047557374356792 1103 1 -0.004015919373145493 1103 2 -0.1818801577658533 1103 3 -0.004362988908553689 1103 4 0.030506457201965986 1103 5 0.07361004254962467 1103 6 0.07645103434492029 1103 7 0.1966433837369408 1103 8 0.2383552202680903 1103 9 0.09951185776913718 1103 10-11 0.22892316629046405 1103 12-13 0.2287447575595749 1103 14-15 0.19803762559182303 1103 16-17 0.3014406802576488 1103 18-19 0.3772838503830016 1103 20-21 0.22630941991811682 1103 22-23 0.2673949815556895 1103 24-25 0.2973661016115656 1103 26-27 0.2952028144834671 1103 28-29 0.41027379406563824 1103 30-31 0.4053976689851666 1103 32-33 0.4752361618927097 1103 34-35 0.36646848876827676 1103 36-37 0.35056072394601046 1103 38-39 0.36338219575650044 1103 40-41 0.36422863401860184 1103 42-43 0.35031674361616183 1103 44-45 0.5311861801031981 1103 46-47 0.5431867121715683 1103 48-49 0.4888162745287232 1103 50-51 0.4126587593301636 1103 52-53 0.546102849080107 1103 54-55 0.3882554571701462 1103 56-57 0.5383231226917005 1103 58-59 0.4914837108972705 1103 60-61 0.5790900670394876 1103 62-63 0.5513788984725778 1103 64-65 0.44226310458163454 1103 66-67 0.42882639617490526 1103 68-69 0.5106934004112205 1103 70-71 0.5169024745682336 1103 72-73 0.5437329213637696 1103 74-75 0.4609115116466853 1103 76-77 0.4521139386582398 1103 78-79 0.5963847558479394 1103 80-81 0.6582966380416657 1103 82-83 0.5735330073368239 1103 84-85 0.5481935044381494 1103 86-87 0.24702487442246124 1103 88-89 0.5758978228438174 1103 90-91 0.7910982976725549 1103 92-93 0.7811152275343964 1103 94-95 0.5935452968429225 1103 96-97 0.7659226455448476 1103 98-99 0.9348914555106091 1103 100-101 1.1287906166408206 1104 1 0.13664065307182938 1104 2 0.06267006020302546 1104 3 0.05623202356921553 1104 4 0.08869832358009688 1104 5 0.04506575207976482 1104 6 0.06573404442121955 1104 7 0.12272157923240457 1104 8 0.10647004708027907 1104 9 0.15800222726335278 1104 10-11 0.14558507258848863 1104 12-13 0.06073678702233565 1104 14-15 0.16812094884437556 1104 16-17 0.15374100239459665 1104 18-19 0.192652392522902 1104 20-21 0.14212230046758378 1104 22-23 0.07444155924266482 1104 24-25 0.04609564322726811 1104 26-27 0.16260610619437443 1104 28-29 0.1677184505798337 1104 30-31 0.1589368630603616 1104 32-33 0.23754815533347795 1104 34-35 0.29953350532316136 1104 36-37 0.23175928396249645 1104 38-39 0.20741935799434685 1104 40-41 0.2027118461585502 1104 42-43 0.2649210505736974 1104 44-45 0.400783721736822 1104 46-47 0.5090509486980537 1104 48-49 0.5084289933376738 1104 50-51 0.43291524799264636 1104 52-53 0.33795348999373687 1104 54-55 0.30967409182921557 1104 56-57 0.3719149160401898 1104 58-59 0.37451893314487705 1104 60-61 0.40185566200716494 1104 62-63 0.48195872257416283 1104 64-65 0.3595645804079268 1104 66-67 0.4321513736611635 1104 68-69 0.43595897297715425 1104 70-71 0.49976318730183067 1104 72-73 0.4287313756440696 1104 74-75 0.4234149279112387 1104 76-77 0.4392729842547318 1104 78-79 0.3932228629909531 1104 80-81 0.6078543717925662 1104 82-83 0.6168442413587876 1104 84-85 0.5607843994770612 1104 86-87 0.6215838402687766 1104 88-89 0.7752071773602331 1104 90-91 0.8782659679369402 1104 92-93 0.74252683924961 1104 94-95 0.6370057824229249 1104 96-97 0.6676227131980887 1104 98-99 0.6943186655537552 1104 100-101 0.8648620580132373 1105 1 0.09539190676194664 1105 2 -0.0910368420410208 1105 3 0.012975291861074822 1105 4 0.06591309098546105 1105 5 0.050825205910769 1105 6 0.015430159963720769 1105 7 0.09940034148999644 1105 8 0.04129462127971806 1105 9 0.039275100426898746 1105 10-11 0.13555517322615174 1105 12-13 0.2090947060206645 1105 14-15 0.20386670266381657 1105 16-17 0.149695800113669 1105 18-19 0.16765540554160197 1105 20-21 0.13347252449048597 1105 22-23 0.11126443177239764 1105 24-25 0.03656097629497168 1105 26-27 0.05289391238944319 1105 28-29 0.08387034986005659 1105 30-31 0.21920802149935525 1105 32-33 0.17738351680207387 1105 34-35 0.2506565713861235 1105 36-37 0.1350908596227498 1105 38-39 0.2073254199230874 1105 40-41 0.02797435718657937 1105 42-43 0.08732701175314617 1105 44-45 0.11491475106158333 1105 46-47 0.24538381073282522 1105 48-49 0.3008496005314214 1105 50-51 0.26919329862914765 1105 52-53 0.2957521189330592 1105 54-55 0.23876437587031774 1105 56-57 0.2590447759163723 1105 58-59 0.24086350480146734 1105 60-61 0.302927804743085 1105 62-63 0.1332762111185417 1105 64-65 0.15629479764609755 1105 66-67 0.02112349817706871 1105 68-69 0.0933957747916665 1105 70-71 -0.024827680706756894 1105 72-73 0.12086774685705137 1105 74-75 0.10410684187721131 1105 76-77 0.06834512396344294 1105 78-79 0.2109344748150015 1105 80-81 0.280475368852958 1105 82-83 0.38750829932832076 1105 84-85 0.38490678801287004 1105 86-87 0.30741226526693666 1105 88-89 0.22388369115853024 1105 90-91 0.3442393848817815 1105 92-93 0.48004542110826876 1105 94-95 0.45583878588510274 1105 96-97 0.7679972924397198 1105 98-99 0.5782378867588989 1105 100-101 0.5923858372109052 1106 1 0.2857988113392196 1106 2 0.22141863469116885 1106 3 0.21469214529372493 1106 4 0.19428798931312485 1106 5 0.1967057528200442 1106 6 0.2571939281083857 1106 7 0.27093843699902465 1106 8 0.22337510208733136 1106 9 0.342928528220412 1106 10-11 0.34920974959478457 1106 12-13 0.3707906421743772 1106 14-15 0.5380258806846498 1106 16-17 0.5597258228869961 1106 18-19 0.5947737328582363 1106 20-21 0.45280730558251037 1106 22-23 0.5016554182492499 1106 24-25 0.5640237482904453 1106 26-27 0.5488887094933332 1106 28-29 0.5559264122590193 1106 30-31 0.5610728813018042 1106 32-33 0.4968324095777845 1106 34-35 0.3979498230704692 1106 36-37 0.5804839972806519 1106 38-39 0.6821293257603855 1106 40-41 0.6055533611482034 1106 42-43 0.572698234853533 1106 44-45 0.6727630884298463 1106 46-47 0.5116205395227027 1106 48-49 0.4785776422364165 1106 50-51 0.5258075676825413 1106 52-53 0.5056444192392107 1106 54-55 0.5557654611565965 1106 56-57 0.5920838268310646 1106 58-59 0.454097753819795 1106 60-61 0.49299365283155794 1106 62-63 0.697166501098593 1106 64-65 0.6970635673133145 1106 66-67 0.760980823411991 1106 68-69 0.916764419884295 1106 70-71 0.8391919997762685 1106 72-73 0.9178416456473428 1106 74-75 0.941492045629964 1106 76-77 0.8911214750788048 1106 78-79 0.7216812169850364 1106 80-81 0.7567397124572004 1106 82-83 0.8645875132938023 1106 84-85 0.9196138938997755 1106 86-87 0.8248090229608458 1106 88-89 0.9847480077851039 1106 90-91 1.0531267352340556 1106 92-93 0.9661792000631735 1106 94-95 0.8022930449998178 1106 96-97 1.2559224436989673 1106 98-99 1.1957274254557575 1106 100-101 1.148453243866804 1107 1 0.23689347219176682 1107 2 0.07102519130674523 1107 3 0.05375129917901944 1107 4 0.18900891992274182 1107 5 0.14302615775757488 1107 6 0.14745768876720433 1107 7 0.16176419703471367 1107 8 0.12522196569259592 1107 9 0.17277549348951027 1107 10-11 0.1606263122970475 1107 12-13 0.18032445218835136 1107 14-15 0.19248708213195442 1107 16-17 0.2536679735413898 1107 18-19 0.30746760073295576 1107 20-21 0.22284165217990903 1107 22-23 0.2347485488859533 1107 24-25 0.30349540752732906 1107 26-27 0.24909928945477589 1107 28-29 0.2784347830918392 1107 30-31 0.43161714647612115 1107 32-33 0.28485369412650385 1107 34-35 0.33331693683886243 1107 36-37 0.24807823679799412 1107 38-39 0.29169006354177185 1107 40-41 0.1894033395227268 1107 42-43 0.34118056484817316 1107 44-45 0.5543257183853498 1107 46-47 0.624806447163742 1107 48-49 0.5574709613893987 1107 50-51 0.6079214828747652 1107 52-53 0.6114820480882415 1107 54-55 0.6864719458625501 1107 56-57 0.5641595786503828 1107 58-59 0.6364440935496205 1107 60-61 0.6612672214202391 1107 62-63 0.5556617406461726 1107 64-65 0.5945112794609599 1107 66-67 0.6174379661940108 1107 68-69 0.6610447342183292 1107 70-71 0.6872061152725095 1107 72-73 0.6991650588700438 1107 74-75 0.7276679345639891 1107 76-77 0.7577122444417093 1107 78-79 0.700686979834007 1107 80-81 0.8124983765594784 1107 82-83 0.8558692925509632 1107 84-85 0.8393890853849051 1107 86-87 0.7914371055767262 1107 88-89 0.772786272821822 1107 90-91 1.0118092640866436 1107 92-93 1.13530573690689 1107 94-95 1.0476011718394602 1107 96-97 1.1726638245128136 1107 98-99 1.1923361481242942 1107 100-101 0.9990588009341685 1108 1 0.085419547426838 1108 2 -0.09512559875885529 1108 3 0.022556995958559867 1108 4 0.01617187630633765 1108 5 -0.004000042437745321 1108 6 0.10214991694345343 1108 7 0.048144624543489556 1108 8 0.11122152842948907 1108 9 0.09852270300385157 1108 10-11 0.047675052271380025 1108 12-13 0.0770471495463525 1108 14-15 0.045719895794896104 1108 16-17 0.10115232222078419 1108 18-19 0.13334516784481565 1108 20-21 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5 39.0 6 78.0 7 206.0 8 433.0 9 899.0 10 1521.0 11 2538.0 12 4034.0 13 5464.0 14 6986.0 15 9342.0 16 12555.0 17 17004.0 18 15429.0 19 9819.0 20 8872.0 21 9336.0 22 10066.0 23 10881.0 24 12905.0 25 15467.0 26 19221.0 27 24317.0 28 30683.0 29 38296.0 30 48790.0 31 61921.0 32 79341.0 33 105094.0 34 143161.0 35 205891.0 36 318832.0 37 484717.0 38 374624.0 39 32979.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.836118995059394 19.51886467373653 15.07109223677394 22.573924094430136 2 15.544516189847762 20.773106471225905 39.80350662204836 23.878870716877977 3 19.52993731020678 24.722382862558955 29.90851277526645 25.839167051967816 4 13.35420588321238 17.284045460243966 37.03113349917003 32.33061515737362 5 13.914117253373426 37.52720657199549 34.17758150188761 14.381094672743469 6 31.798286233262118 36.441791928301 17.385951938933687 14.373969899503194 7 28.25527639043569 32.774489893546075 20.8684476070136 18.10178610900463 8 26.320951509261626 34.9398567042737 19.72479528845311 19.014396498011564 9 26.1283289486211 17.02067994302859 20.415493508709254 36.43549759964105 10-11 24.777832213627956 26.840031888568166 27.407602213913094 20.974533683890783 12-13 25.872085898823237 24.11196030450307 27.90356742918117 22.112386367492526 14-15 22.890436551438754 25.55312414281311 26.25218803581545 25.30425126993269 16-17 22.123481100177873 28.72713107854495 26.793529540957863 22.355858280319318 18-19 22.09843121248997 27.495374136450483 28.998956529607884 21.40723812145166 20-21 23.042926614991465 26.42922184611121 28.999737947715854 21.52811359118147 22-23 22.59881626908905 26.36999084724706 28.990850074890446 22.040342808773445 24-25 22.555336520277 26.482252840028703 29.030967241722642 21.931443397971652 26-27 22.387894741183164 26.594311932875293 29.0922352004091 21.925558125532437 28-29 22.255848464160284 26.91029955137168 28.951432337295348 21.88241964717269 30-31 22.47447833610583 26.584294672516172 28.87946496542667 22.061762025951325 32-33 22.378621192169092 26.60860442296109 28.91408773717495 22.09868664769487 34-35 22.679754226485766 26.694818494237783 28.76437608447339 21.861051194803057 36-37 22.729801810497946 26.51948466346151 28.827795312350133 21.922918213690416 38-39 22.464152684607253 26.762130680626495 28.812343080359355 21.961373554406897 40-41 22.654963232738194 26.751667859523913 28.601849545390596 21.991519362347297 42-43 22.47301877050384 26.792914100041642 28.379231528960297 22.354835600494223 44-45 22.70087083816229 26.734194289710988 28.327997326733744 22.236937545392976 46-47 22.92525671464172 27.026681827018812 27.9439852481133 22.104076210226168 48-49 22.872890576378037 27.112722772172233 27.771068196498327 22.2433184549514 50-51 22.73340613442689 27.28134574879475 27.795800002168008 22.18944811461035 52-53 22.969219689821497 27.389659271185145 27.44442548731092 22.196695551682435 54-55 22.83159526002287 27.17087917319053 27.50466843066261 22.49285713612399 56-57 22.85176524483571 27.444038780078518 27.376547624742575 22.327648350343193 58-59 23.04552826292797 27.204596988222384 27.424393315476898 22.32548143337275 60-61 22.90788949661717 27.17480187298662 27.814157686244105 22.1031509441521 62-63 22.602445321491626 27.210234304819664 27.909722372778152 22.27759800091056 64-65 22.739674506530655 27.327133143522627 27.634542595002365 22.298649754944357 66-67 22.770141186667423 27.29274276539985 27.418746322343353 22.518369725589373 68-69 22.731205985956983 27.266178268480125 27.611645204984903 22.39097054057799 70-71 22.925833087272064 27.1373102019242 27.475354793646694 22.461501917157044 72-73 22.84351605550853 27.065946071287854 27.51821126194996 22.572326611253654 74-75 22.73154127869176 27.62421382869696 27.158043025241614 22.486201867369672 76-77 22.815945576794917 27.34960235504599 27.202553373595844 22.63189869456324 78-79 22.855243486998507 27.317938537701792 27.21024472182475 22.616573253474954 80-81 22.92061165308236 27.461795249166027 27.094694992405593 22.522898105346016 82-83 22.908811889984463 27.455008758230655 27.19715174279717 22.439027608987715 84-85 22.84409520222956 27.46613168004683 27.10917280843938 22.58060030928423 86-87 22.78658978586302 27.436530447381045 27.132532664443946 22.644347102311986 88-89 22.848938664655734 27.36172594394924 26.948573987121407 22.84076140427362 90-91 22.819250442675155 27.204008560821467 27.13470285739332 22.84203813911005 92-93 22.867552659013914 27.207055073042994 27.2054526328093 22.719939635133784 94-95 22.73401991791243 27.291772367119066 27.17272017526971 22.80148753969879 96-97 22.604770416489433 27.22031274118081 27.303829789239636 22.87108705309012 98-99 22.505443747937974 27.364683979827497 27.006527784323893 23.12334448791064 100-101 22.52107525822538 27.36396021852803 26.956252682053872 23.15871184119272 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 213.0 1 163.0 2 125.5 3 143.0 4 136.5 5 155.0 6 261.5 7 543.5 8 952.0 9 1253.0 10 1299.5 11 1303.5 12 1413.0 13 1596.0 14 1786.0 15 1982.5 16 2182.0 17 2351.5 18 2558.0 19 2810.0 20 3210.5 21 3775.0 22 4488.0 23 5640.5 24 6992.5 25 8789.5 26 11403.5 27 14485.5 28 17435.0 29 20443.5 30 24024.5 31 28162.5 32 32332.0 33 36159.5 34 40482.0 35 44585.0 36 49279.5 37 53999.5 38 57305.5 39 60139.5 40 63117.5 41 66316.5 42 68299.5 43 70909.0 44 74717.0 45 77706.5 46 79826.5 47 83475.5 48 88961.5 49 88166.5 50 82343.0 51 78331.5 52 76197.0 53 75252.0 54 73218.5 55 67804.5 56 60365.0 57 53721.0 58 48843.0 59 43793.0 60 36706.5 61 29821.0 62 23838.5 63 18238.5 64 13391.0 65 9588.0 66 6912.5 67 5169.0 68 3768.0 69 2488.5 70 1584.0 71 955.5 72 598.0 73 431.0 74 280.0 75 159.0 76 97.5 77 45.5 78 27.0 79 20.0 80 11.0 81 5.0 82 2.5 83 2.0 84 2.0 85 0.5 86 1.5 87 1.5 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017296912100580365 2 0.0031106163450634987 3 0.002309396983456234 4 0.0 5 0.0016966998245800905 6 3.2991385477946204E-4 7 1.4139165204834087E-4 8 0.0 9 0.0 10-11 1.1782637670695073E-4 12-13 6.598277095589241E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002450788635504575 22-23 0.0 24-25 0.004171053735426055 26-27 0.0020501789547009426 28-29 0.003605487127232692 30-31 0.015694473377365833 32-33 9.426110136556058E-4 34-35 0.0026628761135770863 36-37 0.003982531532694934 38-39 0.002780702490284037 40-41 0.005160795299764442 42-43 0.0028042677656254273 44-45 8.012193616072648E-4 46-47 1.6495692738973102E-4 48-49 3.5347913012085216E-4 50-51 1.4139165204834087E-4 52-53 9.426110136556058E-4 54-55 0.003204877446429059 56-57 3.0634857943807187E-4 58-59 0.0013667859698006284 60-61 9.89741564338386E-4 62-63 0.0 64-65 1.4139165204834087E-4 66-67 2.3565275341390142E-5 68-69 0.0 70-71 2.120874780725113E-4 72-73 9.426110136556057E-5 74-75 5.420013328519734E-4 76-77 0.001131133216386727 78-79 4.713055068278029E-4 80-81 0.00652758126956507 82-83 0.002238701157432064 84-85 0.004383141213498566 86-87 0.0018852220273112117 88-89 0.0013432206944592382 90-91 3.2991385477946204E-4 92-93 9.426110136556057E-5 94-95 3.5347913012085216E-4 96-97 0.0020501789547009426 98-99 0.0031106163450634987 100-101 0.001154698491728117 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2121766.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.71858436610511 #Duplication Level Percentage of deduplicated Percentage of total 1 76.75141429223143 46.6023722392074 2 13.675388908049541 16.607005103054078 3 4.273348361737436 7.784149890837345 4 1.9202682466809518 4.663838781666003 5 0.9732453622478883 2.9547040318284465 6 0.5552085650119868 2.0226886857278683 7 0.37574484126855806 1.5970286391285602 8 0.26139472151775084 1.2697213961064082 9 0.1958667845076042 1.0703478491678426 >10 0.9533894919715201 10.020031647369164 >50 0.04224797988081587 1.7175249516108992 >100 0.020850418524730702 2.158494054197585 >500 0.0010103020384679968 0.4252562425664512 >1k 4.662932485236475E-4 0.5704058433358744 >5k 1.5543108284121583E-4 0.5364306441960803 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6229 0.29357620020303843 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5139 0.2422038999588079 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3458 0.16297744426105423 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3154 0.14864975685348902 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 4.7130550682780285E-5 10-11 0.0 0.0 0.0 0.0 4.7130550682780285E-5 12-13 0.0 0.0 0.0 0.0 4.7130550682780285E-5 14-15 0.0 0.0 0.0 0.0 4.7130550682780285E-5 16-17 2.3565275341390142E-5 0.0 0.0 0.0 4.7130550682780285E-5 18-19 4.7130550682780285E-5 0.0 0.0 0.0 4.7130550682780285E-5 20-21 4.7130550682780285E-5 0.0 0.0 0.0 4.7130550682780285E-5 22-23 4.7130550682780285E-5 0.0 0.0 2.3565275341390142E-5 4.7130550682780285E-5 24-25 4.7130550682780285E-5 0.0 0.0 9.426110136556058E-5 4.7130550682780285E-5 26-27 4.7130550682780285E-5 0.0 0.0 4.713055068278029E-4 4.7130550682780285E-5 28-29 4.7130550682780285E-5 0.0 0.0 0.0010604373903625565 4.7130550682780285E-5 30-31 4.7130550682780285E-5 0.0 0.0 0.002191570606749283 4.7130550682780285E-5 32-33 7.069582602417042E-5 0.0 0.0 0.003440530199842961 4.7130550682780285E-5 34-35 9.426110136556057E-5 0.0 0.0 0.004642359242253858 4.7130550682780285E-5 36-37 9.426110136556057E-5 0.0 0.0 0.0056792313572750245 4.7130550682780285E-5 38-39 1.8852220273112114E-4 0.0 0.0 0.00810645471743821 7.069582602417042E-5 40-41 1.8852220273112114E-4 0.0 0.0 0.011028548859770587 9.426110136556057E-5 42-43 1.8852220273112114E-4 0.0 0.0 0.014728297088368841 9.426110136556057E-5 44-45 2.8278330409668175E-4 0.0 0.0 0.02278762125512427 9.426110136556057E-5 46-47 2.8278330409668175E-4 0.0 0.0 0.03819931132839342 9.426110136556057E-5 48-49 3.2991385477946204E-4 0.0 0.0 0.049675600419650426 9.426110136556057E-5 50-51 3.2991385477946204E-4 0.0 0.0 0.06320206846560836 9.426110136556057E-5 52-53 3.2991385477946204E-4 0.0 0.0 0.08191289708667214 9.426110136556057E-5 54-55 3.2991385477946204E-4 0.0 0.0 0.10472408361713781 9.426110136556057E-5 56-57 3.2991385477946204E-4 0.0 0.0 0.12751170487226207 9.426110136556057E-5 58-59 3.2991385477946204E-4 0.0 0.0 0.15807586699004508 9.426110136556057E-5 60-61 3.2991385477946204E-4 0.0 0.0 0.19328238835008196 9.426110136556057E-5 62-63 3.2991385477946204E-4 0.0 0.0 0.23508718680570806 9.426110136556057E-5 64-65 3.2991385477946204E-4 0.0 0.0 0.26793718063160593 9.426110136556057E-5 66-67 3.2991385477946204E-4 0.0 0.0 0.3253186260878909 9.426110136556057E-5 68-69 3.2991385477946204E-4 0.0 0.0 0.3975933255599345 9.426110136556057E-5 70-71 4.0060968080363245E-4 0.0 0.0 0.4437341346783764 9.426110136556057E-5 72-73 4.2417495614502257E-4 0.0 0.0 0.5256470317650486 9.426110136556057E-5 74-75 4.2417495614502257E-4 0.0 0.0 0.5943869399358837 9.426110136556057E-5 76-77 4.2417495614502257E-4 0.0 0.0 0.6515327326387548 9.426110136556057E-5 78-79 4.2417495614502257E-4 0.0 0.0 0.7213802087506351 9.426110136556057E-5 80-81 4.7130550682780286E-4 0.0 0.0 0.7981087452622013 9.426110136556057E-5 82-83 4.7130550682780286E-4 0.0 0.0 0.8948442005386079 9.426110136556057E-5 84-85 4.7130550682780286E-4 0.0 0.0 1.0440595240002903 9.426110136556057E-5 86-87 4.7130550682780286E-4 0.0 0.0 1.2632165846752188 9.426110136556057E-5 88-89 4.7130550682780286E-4 0.0 0.0 1.5226231356332414 9.426110136556057E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12080 0.0 26.941626 1 GGTATCA 8850 0.0 26.305891 1 TCAACGC 14110 0.0 22.890709 4 ATCAACG 14205 0.0 22.772131 3 TATCAAC 14400 0.0 22.530794 2 CAACGCA 14395 0.0 22.470503 5 AACGCAG 14785 0.0 21.877258 6 GTACATG 8495 0.0 21.64451 1 GAGTACT 7330 0.0 21.57781 12-13 TACATGG 8500 0.0 20.789415 2 GTACTTT 7590 0.0 20.651157 14-15 AGAGTAC 11775 0.0 20.632498 10-11 ACGCAGA 16305 0.0 19.779535 7 ACATGGG 8495 0.0 19.682358 3 CGCAGAG 16365 0.0 19.532875 8 AGTACTT 7715 0.0 19.515982 12-13 GCAGAGT 17325 0.0 18.12155 9 ACTTTTT 9325 0.0 17.012768 16-17 TACTTTT 8805 0.0 16.749601 14-15 GTGTACG 300 1.4636789E-6 15.83722 1 >>END_MODULE