FastQCFastQC Report
Thu 26 May 2016
SRR936986_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936986_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887717
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56160.2975022209367188No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40510.21459784491001566No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25940.13741466543978784No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25920.13730871735540867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA98250.028.0092541
GGTATCA70800.025.9796471
ATCAACG114900.023.6615413
TCAACGC114950.023.6099014
CAACGCA115550.023.5714475
GTACATG71450.023.5481381
TATCAAC115900.023.4983982
AACGCAG119100.022.8277346
TACATGG72450.022.6332932
GAGTACT57950.022.34924312-13
ACATGGG72600.021.7354433
GTACTTT59950.021.24632514-15
ACGCAGA130600.020.7453987
CGCAGAG131050.020.6016228
AGAGTAC94750.020.29059610-11
CATGGGG36900.019.9651414
AGTACTT60000.019.68452512-13
GCAGAGT136350.019.0338929
ATGGGGA20800.017.1395555
GTCTAGG7500.017.1103151
Produced by FastQC (version 0.11.5)