##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936986_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1887717 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.737526864461145 33.0 31.0 34.0 30.0 34.0 2 31.862743197205937 33.0 31.0 34.0 30.0 34.0 3 31.8806261743683 33.0 31.0 34.0 30.0 34.0 4 35.46339626119805 37.0 35.0 37.0 33.0 37.0 5 35.46702233438593 37.0 35.0 37.0 33.0 37.0 6 35.52451188393175 37.0 35.0 37.0 33.0 37.0 7 35.45371260628579 37.0 35.0 37.0 33.0 37.0 8 35.40303234012302 37.0 35.0 37.0 33.0 37.0 9 37.037272006344175 39.0 37.0 39.0 33.0 39.0 10-11 36.854317410925475 39.0 37.0 39.0 33.0 39.0 12-13 36.79209118739726 39.0 37.0 39.0 33.0 39.0 14-15 37.964014998010825 40.0 38.0 41.0 33.0 41.0 16-17 37.88840567733405 40.0 38.0 41.0 33.0 41.0 18-19 37.84355626399508 40.0 38.0 41.0 32.5 41.0 20-21 37.76249776846847 40.0 37.5 41.0 32.0 41.0 22-23 37.64839989256865 40.0 37.0 41.0 32.0 41.0 24-25 37.49135834449761 40.0 37.0 41.0 32.0 41.0 26-27 37.59309128433976 40.0 37.0 41.0 32.0 41.0 28-29 37.55482839853643 40.0 37.0 41.0 32.0 41.0 30-31 37.4144101049045 40.0 37.0 41.0 31.5 41.0 32-33 37.22553460079027 40.0 36.5 41.0 31.0 41.0 34-35 37.062875685285455 40.0 36.0 41.0 31.0 41.0 36-37 36.895472414562136 39.0 36.0 41.0 30.0 41.0 38-39 36.743044640695615 39.0 36.0 41.0 30.0 41.0 40-41 36.58128601903781 39.0 36.0 41.0 30.0 41.0 42-43 36.37499264985165 39.0 35.0 41.0 30.0 41.0 44-45 36.13632154607921 39.0 35.0 41.0 29.5 41.0 46-47 35.81066415145914 39.0 35.0 40.0 28.0 41.0 48-49 35.508778858271654 39.0 35.0 40.0 27.0 41.0 50-51 34.884272112821996 38.0 34.0 39.5 26.5 40.5 52-53 35.123453091750505 38.5 34.5 40.0 27.0 40.5 54-55 35.51018293525989 39.0 35.0 40.5 27.5 41.0 56-57 35.392541890548216 39.0 35.0 41.0 27.0 41.0 58-59 35.15322847651423 38.5 35.0 40.5 26.0 41.0 60-61 34.868672316878005 38.0 34.0 40.0 26.0 41.0 62-63 34.55150692609115 38.0 34.0 40.0 26.0 41.0 64-65 34.23490226554087 37.0 34.0 40.0 25.5 41.0 66-67 33.856923733801196 37.0 33.5 39.5 24.5 41.0 68-69 33.467352892409195 36.0 33.0 39.0 23.0 41.0 70-71 33.04758790644996 36.0 33.0 39.0 22.5 40.5 72-73 32.64772076534777 35.0 32.0 38.5 22.0 40.0 74-75 32.21428106013772 35.0 32.0 37.5 20.5 39.5 76-77 31.779474624639178 35.0 31.5 37.0 20.0 39.0 78-79 31.334041066536987 35.0 31.0 36.5 18.0 39.0 80-81 30.902321958217254 35.0 31.0 36.0 17.0 38.0 82-83 30.490905151566682 34.5 31.0 35.5 12.0 37.0 84-85 30.13140979288739 34.0 30.0 35.0 7.5 37.0 86-87 29.808937992294396 34.0 30.0 35.0 7.0 36.0 88-89 29.451606623238547 34.0 30.0 35.0 2.0 36.0 90-91 29.100747092917 34.0 29.0 35.0 2.0 36.0 92-93 28.733176371246323 34.0 29.0 35.0 2.0 35.0 94-95 28.308517908139834 34.0 29.0 35.0 2.0 35.0 96-97 27.826451210642276 34.0 28.5 35.0 2.0 35.0 98-99 27.220937248538846 34.0 27.0 35.0 2.0 35.0 100-101 24.98654644737532 31.5 21.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3162890973914969 1101 2 0.31559039152985946 1101 3 0.20911810564877698 1101 4 0.23091930290907214 1101 5 0.22951442959996626 1101 6 0.16222578932893583 1101 7 0.14053409419338436 1101 8 0.219732409953437 1101 9 0.25605474235062076 1101 10-11 0.25507156034160516 1101 12-13 0.2536645295201936 1101 14-15 0.4558123976582422 1101 16-17 0.5923101318652115 1101 18-19 0.47806195980486166 1101 20-21 0.5761569266310858 1101 22-23 0.6109186171804524 1101 24-25 0.553163044141094 1101 26-27 0.5624441883012565 1101 28-29 0.5974709394223652 1101 30-31 0.561552727517487 1101 32-33 0.6514820410681281 1101 34-35 0.45761133989400093 1101 36-37 0.3620291138639402 1101 38-39 0.40291484801662136 1101 40-41 0.49285313972512057 1101 42-43 0.31815474886770545 1101 44-45 0.4062319705714188 1101 46-47 0.3135054363698586 1101 48-49 0.4696317187738046 1101 50-51 0.5355979338496368 1101 52-53 0.5167402914426731 1101 54-55 0.5606418331062528 1101 56-57 0.7430475436027493 1101 58-59 0.4730436675379224 1101 60-61 0.5621471409195706 1101 62-63 0.5963965841505896 1101 64-65 0.678371723052031 1101 66-67 0.5361522211326459 1101 68-69 0.46398214636659674 1101 70-71 0.34401040809733985 1101 72-73 0.45197364944009877 1101 74-75 0.3585335317778444 1101 76-77 0.47605303263428667 1101 78-79 0.49705073150332524 1101 80-81 0.24598967780049819 1101 82-83 0.4837537505339 1101 84-85 0.44962857242990495 1101 86-87 0.535673226115037 1101 88-89 0.5269015446684513 1101 90-91 0.5641569892890068 1101 92-93 0.6799398972332256 1101 94-95 0.7018502615654505 1101 96-97 0.6145590014667199 1101 98-99 0.8389083686296743 1101 100-101 1.0121127277421742 1102 1 0.276495245021966 1102 2 0.29253792244589505 1102 3 0.192660726950038 1102 4 0.30847011453616346 1102 5 0.15381957267872792 1102 6 0.08124859978335763 1102 7 0.22357031699345242 1102 8 0.17071718822372617 1102 9 0.179953346605501 1102 10-11 0.3796232224555425 1102 12-13 0.45379118640448013 1102 14-15 0.44520364298781345 1102 16-17 0.4176530368656799 1102 18-19 0.37435352410518163 1102 20-21 0.3209927383879574 1102 22-23 0.46956067825975367 1102 24-25 0.5099794596062139 1102 26-27 0.3532167876608696 1102 28-29 0.36338435772294986 1102 30-31 0.33284200145823206 1102 32-33 0.4226342751612009 1102 34-35 0.2971508478331515 1102 36-37 0.2264239855961776 1102 38-39 0.3148503316413169 1102 40-41 0.30759206163759956 1102 42-43 0.3138883815764828 1102 44-45 0.23974077089633994 1102 46-47 0.2655848156518772 1102 48-49 0.34020979789504224 1102 50-51 0.2165529450800321 1102 52-53 0.24191756527348218 1102 54-55 0.27274936090740454 1102 56-57 0.4937243448560267 1102 58-59 0.4490288657074615 1102 60-61 0.3200353301460197 1102 62-63 0.3251422381933011 1102 64-65 0.4179490600967597 1102 66-67 0.29605707196152764 1102 68-69 0.012627475025560386 1102 70-71 0.25453598454267023 1102 72-73 0.22319650295328586 1102 74-75 0.061600628624937315 1102 76-77 0.17873382216704314 1102 78-79 0.09090966673043255 1102 80-81 0.11826644027036437 1102 82-83 -0.025964817716523925 1102 84-85 0.03198455843733328 1102 86-87 -0.009239007306195646 1102 88-89 0.07909559461966964 1102 90-91 0.08469998773614051 1102 92-93 0.24582609842757108 1102 94-95 -0.005543515374906605 1102 96-97 0.09750364805660183 1102 98-99 0.23350563943720815 1102 100-101 0.2727690073737037 1103 1 0.23468729513145803 1103 2 0.1868214171251381 1103 3 0.23295079305838584 1103 4 0.1310415286318971 1103 5 0.028704779082026732 1103 6 0.029995018054258082 1103 7 -0.12178811862361272 1103 8 0.20213000795227742 1103 9 0.11748967992819814 1103 10-11 0.21050434188779832 1103 12-13 0.17463098577135838 1103 14-15 0.22497782751700157 1103 16-17 0.2954025944857719 1103 18-19 0.18957158033574473 1103 20-21 0.28162908991250646 1103 22-23 0.3792300802071722 1103 24-25 0.2244965571437092 1103 26-27 0.3275793008665886 1103 28-29 0.33125021950527156 1103 30-31 0.1605859296416341 1103 32-33 0.2751488507110551 1103 34-35 0.28684672689783497 1103 36-37 0.1535001687630313 1103 38-39 0.2827909711077936 1103 40-41 0.2848805683919906 1103 42-43 0.27917774400524564 1103 44-45 0.3516340818120014 1103 46-47 0.3930000785559571 1103 48-49 0.6335004475883252 1103 50-51 0.5964349333220085 1103 52-53 0.5371666959946282 1103 54-55 0.4049862363448611 1103 56-57 0.5435170877615363 1103 58-59 0.6189732093432951 1103 60-61 0.6110620080465452 1103 62-63 0.7276530607853644 1103 64-65 0.5979227143330021 1103 66-67 0.5810556552199628 1103 68-69 0.6056548547291882 1103 70-71 0.5054768510631007 1103 72-73 0.5439963333195905 1103 74-75 0.34533296392169177 1103 76-77 0.542837812874712 1103 78-79 0.5708498258183106 1103 80-81 0.4951135053635376 1103 82-83 0.5979834627720351 1103 84-85 0.4024904383839676 1103 86-87 0.495573035184794 1103 88-89 0.5359624753300274 1103 90-91 0.5990101096509761 1103 92-93 0.7424690776755902 1103 94-95 0.6224201484876772 1103 96-97 0.7421433922549987 1103 98-99 0.7813681423222967 1103 100-101 0.8966898912703236 1104 1 0.20528333165906432 1104 2 0.13768440071802956 1104 3 0.30899092037451936 1104 4 0.24552942223198926 1104 5 0.14457856181098805 1104 6 0.1202615862024885 1104 7 0.15619869506286932 1104 8 0.14761142207072453 1104 9 0.24986814228278575 1104 10-11 0.4016618387617257 1104 12-13 0.3483805594896694 1104 14-15 0.3617218879921609 1104 16-17 0.366749360428706 1104 18-19 0.42559996122316335 1104 20-21 0.34492292114285306 1104 22-23 0.29407273490005537 1104 24-25 0.37992359666476005 1104 26-27 0.25017080933538693 1104 28-29 0.26964588469415673 1104 30-31 0.5324878090702256 1104 32-33 0.5835631775649119 1104 34-35 0.4525755738532382 1104 36-37 0.33770974778618523 1104 38-39 0.37444153379961875 1104 40-41 0.4333577954769723 1104 42-43 0.3757916464719955 1104 44-45 0.38976687329395077 1104 46-47 0.5756300471058395 1104 48-49 0.47500086439709577 1104 50-51 0.40316260563454165 1104 52-53 0.3812887451780682 1104 54-55 0.5417667675865729 1104 56-57 0.42252176738716685 1104 58-59 0.4720462266597991 1104 60-61 0.48648307962397297 1104 62-63 0.4017152410740934 1104 64-65 0.5297048559722057 1104 66-67 0.4544248909442601 1104 68-69 0.45628859350377127 1104 70-71 0.42429245107825153 1104 72-73 0.4257661601544953 1104 74-75 0.4396037380492359 1104 76-77 0.3339863104685925 1104 78-79 0.43711910322348757 1104 80-81 0.506565326059988 1104 82-83 0.47136359236166925 1104 84-85 0.3335242188394254 1104 86-87 0.41321115045136025 1104 88-89 0.459249770241172 1104 90-91 0.5819228450531959 1104 92-93 0.6891450278101061 1104 94-95 0.7052125777249181 1104 96-97 0.6584178797696794 1104 98-99 0.7128026275634767 1104 100-101 0.6970844386538992 1105 1 0.14950285482595405 1105 2 0.29007981232396673 1105 3 0.20139982725711292 1105 4 0.2316517794069739 1105 5 0.2192411798946594 1105 6 0.251064555163353 1105 7 0.31834444405072304 1105 8 0.3597571710585825 1105 9 0.2329540622108155 1105 10-11 0.26817668356604685 1105 12-13 0.297582088954357 1105 14-15 0.3571728551536566 1105 16-17 0.2599061305591661 1105 18-19 0.25122974080031923 1105 20-21 0.32697982622607213 1105 22-23 0.2924476786697383 1105 24-25 0.3461516668401998 1105 26-27 0.17643599152162182 1105 28-29 0.2370772837045081 1105 30-31 0.07906338261948065 1105 32-33 0.10445274121763504 1105 34-35 0.10347525896345644 1105 36-37 -0.04977338311800139 1105 38-39 -0.13754677029394458 1105 40-41 -0.0723833201325661 1105 42-43 0.199301543008211 1105 44-45 0.22608603658679982 1105 46-47 0.2193603664940582 1105 48-49 0.2286502121253804 1105 50-51 0.22981577702456235 1105 52-53 0.1268915342019028 1105 54-55 0.2515845813517572 1105 56-57 0.3341220822769557 1105 58-59 0.13347222755948707 1105 60-61 0.3298310328403531 1105 62-63 0.2623652635662239 1105 64-65 0.2786196108080148 1105 66-67 0.24565858415163433 1105 68-69 0.20483785126355514 1105 70-71 0.056473062864128565 1105 72-73 0.07763862979012259 1105 74-75 0.1813491271630454 1105 76-77 0.3125738048455595 1105 78-79 0.18785572040747311 1105 80-81 0.25212313082831983 1105 82-83 0.4372767727485325 1105 84-85 0.2727898508682145 1105 86-87 0.15109351662320236 1105 88-89 0.033260116619938884 1105 90-91 0.07767183290744839 1105 92-93 0.17973207234452104 1105 94-95 0.1799426721918529 1105 96-97 0.1678195891713905 1105 98-99 0.31676686508034635 1105 100-101 0.489566444951695 1106 1 0.25535341043727655 1106 2 0.2294235626725012 1106 3 0.21375748554778085 1106 4 0.27815471825441307 1106 5 0.23227731972505694 1106 6 0.17674387318717066 1106 7 0.23051361459310016 1106 8 0.27192634160095963 1106 9 0.16242915915002243 1106 10-11 0.3022327391532045 1106 12-13 0.41925799412308606 1106 14-15 0.4371695463943652 1106 16-17 0.4272847381291598 1106 18-19 0.38131138164750666 1106 20-21 0.3586323841944221 1106 22-23 0.20607976694297747 1106 24-25 0.33193050788219836 1106 26-27 0.21618199777480385 1106 28-29 0.28746522643695016 1106 30-31 0.477446365930696 1106 32-33 0.4759529847274493 1106 34-35 0.3967976994150746 1106 36-37 0.3504266295222038 1106 38-39 0.147299298044949 1106 40-41 0.13089718458335398 1106 42-43 0.2368429489706685 1106 44-45 0.2859336849511962 1106 46-47 0.42035541079053473 1106 48-49 0.6113119416770658 1106 50-51 0.6175940009271841 1106 52-53 0.6602542374210714 1106 54-55 0.5906515484091486 1106 56-57 0.638511207240235 1106 58-59 0.7479367104450247 1106 60-61 0.5941005219902209 1106 62-63 0.5601935649830452 1106 64-65 0.49415739572378214 1106 66-67 0.6701231413913149 1106 68-69 0.5130004488998381 1106 70-71 0.39644157388527645 1106 72-73 0.4938470890607789 1106 74-75 0.4390745224482515 1106 76-77 0.5505925643954193 1106 78-79 0.4982126946400456 1106 80-81 0.5490173583445568 1106 82-83 0.8115151075131841 1106 84-85 0.8384436282444803 1106 86-87 0.8119503365709306 1106 88-89 0.7363956307602386 1106 90-91 0.9103917445747278 1106 92-93 0.8457118926305611 1106 94-95 1.0831317213139187 1106 96-97 1.0462821685909098 1106 98-99 0.92133109404649 1106 100-101 0.8329255048179327 1107 1 0.16828814840627615 1107 2 0.15826196958308714 1107 3 0.12474492965537465 1107 4 0.13474944579477466 1107 5 0.09644895845128332 1107 6 0.0851380017731671 1107 7 0.16460556193310794 1107 8 0.16216264709537143 1107 9 0.33190832725870223 1107 10-11 0.367206367626153 1107 12-13 0.2534507669864894 1107 14-15 0.21035710955451492 1107 16-17 0.2468024368154289 1107 18-19 0.37126228400133243 1107 20-21 0.49456457037786095 1107 22-23 0.1331946803530002 1107 24-25 0.2834938802998366 1107 26-27 0.3472828648281592 1107 28-29 0.29486448166625934 1107 30-31 0.3020526388010154 1107 32-33 0.24169284103376754 1107 34-35 0.1346778716259962 1107 36-37 0.043903195086478775 1107 38-39 -0.15367873260417042 1107 40-41 0.11699618278833412 1107 42-43 0.047157146219539925 1107 44-45 0.13687572296495176 1107 46-47 0.006625835457484186 1107 48-49 0.23059291956928263 1107 50-51 -0.022605389043789614 1107 52-53 0.020928130301442138 1107 54-55 0.03723593960295091 1107 56-57 0.14163056897083948 1107 58-59 0.1877367205967886 1107 60-61 0.21698076341774453 1107 62-63 0.1663476134623565 1107 64-65 0.08210170865667266 1107 66-67 0.10267040479741496 1107 68-69 -0.036692460089547296 1107 70-71 -0.19359192695225147 1107 72-73 0.051792924759013204 1107 74-75 0.069578973076041 1107 76-77 0.14820413002891542 1107 78-79 0.172877340181806 1107 80-81 0.11073549909421843 1107 82-83 0.1742758810779783 1107 84-85 -0.05460171786162604 1107 86-87 -0.1158140593296011 1107 88-89 -0.18896118084600388 1107 90-91 -0.055879003103054714 1107 92-93 -0.06702557801006037 1107 94-95 0.037568736509268064 1107 96-97 -0.08088950243640625 1107 98-99 0.02664646797767034 1107 100-101 0.00657497346511704 1108 1 0.1897676156325545 1108 2 0.1758264375417724 1108 3 0.2579355345264531 1108 4 0.20569017139318646 1108 5 0.1897615120505236 1108 6 0.0559030805186822 1108 7 0.11264869255898446 1108 8 0.17737255182183986 1108 9 0.18854141919533873 1108 10-11 0.09138278122254917 1108 12-13 0.1854267310555997 1108 14-15 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58-59 -1.5781550502785393 2316 60-61 -1.7139131550930173 2316 62-63 -1.5780788947457083 2316 64-65 -1.9218012104211013 2316 66-67 -1.5961847909445623 2316 68-69 -1.5048541914201152 2316 70-71 -1.3828184759190663 2316 72-73 -1.7799185090960066 2316 74-75 -1.9463901955574592 2316 76-77 -2.235436445995674 2316 78-79 -2.6625099386982853 2316 80-81 -2.7959281675459557 2316 82-83 -2.588641743817629 2316 84-85 -2.779079228145168 2316 86-87 -3.4627472554352856 2316 88-89 -3.2802075309627448 2316 90-91 -2.6644279572460405 2316 92-93 -2.6730556312548437 2316 94-95 -3.2531610109960845 2316 96-97 -3.5391621161111644 2316 98-99 -3.6087333027104442 2316 100-101 -3.554412266021341 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3923.0 3 1487.0 4 1538.0 5 1620.0 6 1812.0 7 2082.0 8 2580.0 9 3226.0 10 3971.0 11 4899.0 12 6406.0 13 7488.0 14 8348.0 15 9883.0 16 12243.0 17 15984.0 18 14224.0 19 10081.0 20 10048.0 21 10641.0 22 11785.0 23 13228.0 24 15005.0 25 16865.0 26 20217.0 27 24025.0 28 28356.0 29 34385.0 30 42432.0 31 52220.0 32 65770.0 33 86267.0 34 118951.0 35 169954.0 36 258583.0 37 384229.0 38 349198.0 39 63721.0 40 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.57830059789734 18.79661633136295 14.695810054977725 22.92927301576198 2 15.060238090569387 20.085917382159053 40.91399305432265 23.939851472948902 3 19.233241328368702 24.688310883168178 29.816761930398037 26.261685858065075 4 12.75397312894471 16.685525128215456 37.826805655736536 32.733696087103304 5 13.794231222850359 37.16443108662177 34.48973912490359 14.55159856562428 6 31.36911283811971 36.076775020818985 18.017290229149342 14.536821911911959 7 27.947842229050835 32.59717562670056 21.31383557018341 18.14114657406519 8 25.856788149681098 34.683672733749496 20.1637030974352 19.29583601913421 9 25.868968401679123 17.132821722792045 20.726906095593815 36.271303779935025 10-11 24.488055715696213 26.570449741234786 27.86375895407166 21.077735588997342 12-13 25.635336914906365 23.991556054586653 28.099576848491402 22.273530182015584 14-15 22.73474356660255 25.313907175236977 26.522780783020583 25.42856847513989 16-17 21.939102997305824 28.540341905620565 27.01653874225417 22.504016354819438 18-19 21.904194044623583 27.33189967577079 29.190910982055428 21.572995297550197 20-21 22.852602742339894 26.337810967292434 29.06867563465539 21.74091065571229 22-23 22.431206284836016 26.231946609310196 29.04410391249641 22.292743193357374 24-25 22.28391800983047 26.368538256657185 29.112237647935224 22.23530608557712 26-27 22.082381105395903 26.482925858806507 29.157976634730442 22.276716401067148 28-29 21.996764320741807 26.802757347633126 29.06704404778336 22.13343428384171 30-31 22.251302798022333 26.405925161432513 29.001295910517538 22.341476130027612 32-33 22.10114618569427 26.481684074455714 29.037410632216638 22.37975910763338 34-35 22.465547530950534 26.535156034771408 28.843784039230147 22.155512395047914 36-37 22.457914339758485 26.320632694756352 28.982042621655918 22.239410343829242 38-39 22.188295421524238 26.6042333477523 28.93194681706891 22.275524413654555 40-41 22.423140965225826 26.52445275138153 28.79948335937198 22.252922924020663 42-43 22.11665502952373 26.583490231686863 28.57076636678509 22.72908837200432 44-45 22.32665604346472 26.547445240007374 28.557095119330906 22.568803597197 46-47 22.56987578873649 26.777547570524735 28.21569542573891 22.43688121499986 48-49 22.53526834561853 26.812974799299997 28.062984400903634 22.588772454177835 50-51 22.352524529579327 27.051698462454553 28.105353887622446 22.49042312034367 52-53 22.540704067055923 27.117131984031733 27.73666924752009 22.605494701392256 54-55 22.402114238155356 26.898446319976394 27.834776007200745 22.86466343466751 56-57 22.510368002492452 27.12265213159349 27.708807887682756 22.6581719782313 58-59 22.700860478693592 26.92752101396834 27.691101817484427 22.68051668985364 60-61 22.47457540906649 26.969803099166768 28.083350906511047 22.472270585255696 62-63 22.30208471027913 26.968278612679708 28.203337729846744 22.52629894719442 64-65 22.35656213212739 27.054073498761884 27.985072650039044 22.604291719071682 66-67 22.360883249669588 27.043924857073147 27.7112921286849 22.883899764572366 68-69 22.270008395918563 26.993129865440107 27.99764628146118 22.739215457180155 70-71 22.496723565925933 26.843287237630285 27.798493277448728 22.861495918995054 72-73 22.37107893432476 26.792963069812714 27.925508593766352 22.91044940209618 74-75 22.31182097792425 27.32537161598313 27.560514261947212 22.80229314414541 76-77 22.401926017261985 27.0380278193662 27.56976500501666 22.99028115835515 78-79 22.429228516170983 27.00894247052143 27.59137289670173 22.970456116605853 80-81 22.49848050730655 27.115512708893814 27.47004334064774 22.915963443151895 82-83 22.518652564197676 27.057001600305224 27.647498330807462 22.776847504689638 84-85 22.450950860653208 27.118821882816157 27.440363076357354 22.989864180173278 86-87 22.389447656219573 27.116616624440088 27.557207233104226 22.936728486236113 88-89 22.450081993488208 26.95174968010618 27.393189904336513 23.204978422069097 90-91 22.39322402628625 26.81953157680153 27.642341252358765 23.144903144553453 92-93 22.40704908003581 26.889587605183902 27.65016799484291 23.053195319937377 94-95 22.288982027044213 26.929522911279726 27.62931308549871 23.152181976177356 96-97 22.104235083515146 26.914722290083375 27.80085351370916 23.18018911269232 98-99 22.10565175092894 27.002910109908925 27.475799657443943 23.415638481718197 100-101 22.2543302810505 26.768429452987696 27.530735964238755 23.44650430172305 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 139.0 1 102.0 2 83.5 3 113.0 4 123.0 5 146.0 6 251.0 7 492.5 8 881.0 9 1137.5 10 1130.0 11 1153.0 12 1286.5 13 1397.5 14 1516.5 15 1692.0 16 1813.0 17 1931.0 18 2118.0 19 2367.5 20 2680.5 21 3118.5 22 3842.5 23 4912.0 24 6147.5 25 7839.0 26 10262.5 27 13126.5 28 15724.5 29 18416.0 30 21836.0 31 25540.0 32 29375.0 33 32952.5 34 36596.5 35 40113.5 36 44439.0 37 48707.0 38 51556.5 39 53985.5 40 56476.0 41 59110.5 42 61017.5 43 63130.5 44 66315.0 45 68913.5 46 71009.5 47 74367.5 48 79027.5 49 78637.0 50 73087.5 51 69516.5 52 67826.5 53 66302.5 54 64176.0 55 59569.5 56 53442.0 57 47770.0 58 43011.0 59 38159.0 60 32321.5 61 26546.5 62 21044.5 63 15903.5 64 11617.5 65 8406.0 66 6022.0 67 4544.0 68 3297.0 69 2209.5 70 1415.0 71 866.5 72 570.0 73 375.5 74 247.0 75 158.5 76 102.0 77 58.5 78 41.5 79 23.5 80 18.0 81 13.5 82 13.0 83 10.0 84 8.0 85 8.5 86 7.0 87 5.5 88 6.5 89 5.5 90 3.0 91 2.0 92 2.5 93 1.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04084298652817133 2 0.0417435452453943 3 0.02648702109479334 4 0.04523983202990703 5 0.06261531786809145 6 0.06452238338691657 7 0.06409859104939988 8 0.06637647486355211 9 0.06653539699012087 10-11 0.07175334014579517 12-13 0.07389878885447342 14-15 0.0684159754878512 16-17 0.05594058855220354 18-19 0.04950424242616875 20-21 0.0552784130248337 22-23 0.03027466511134879 24-25 0.016951693500667737 26-27 0.013640815863818571 28-29 0.01713710264833129 30-31 0.012766744167690391 32-33 0.008052054412817176 34-35 0.010012093973831883 36-37 0.005747683577570155 38-39 0.008290437602670316 40-41 0.0073104178221629615 42-43 0.004396845501735695 44-45 0.001324351054739667 46-47 0.0024368059407209874 48-49 6.092014851802469E-4 50-51 0.005191456134579495 52-53 0.005217943155674288 54-55 0.007098521653404616 56-57 0.022249097719626406 58-59 0.00863476887690263 60-61 0.019653369652336657 62-63 0.012528360977837251 64-65 0.00927045738317767 66-67 0.015918699677970796 68-69 0.02598376769399227 70-71 0.0198917528421898 72-73 0.017560894985847984 74-75 0.01864686285073451 76-77 0.026831352369025653 78-79 0.037638056975701335 80-81 0.030407100216822757 82-83 0.030566022343391515 84-85 0.021216103896929465 86-87 0.02529510514552764 88-89 0.019706343694526243 90-91 0.018143609449933436 92-93 0.03207578254579473 94-95 0.023149656436849377 96-97 0.036763985279573155 98-99 0.05395406197009403 100-101 0.06386020785954674 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1887717.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.880297011226894 #Duplication Level Percentage of deduplicated Percentage of total 1 78.95256547292789 50.43513332208972 2 12.618333372507665 16.121257672449318 3 3.8058737400075424 7.2936103469673235 4 1.6935076624122585 4.327270898627346 5 0.8384935331846095 2.6781607970912944 6 0.49865245760387505 1.9112440258268262 7 0.3310426543111043 1.480297215654478 8 0.23630014727783977 1.207593887352405 9 0.16359180481863897 0.9405263772375584 >10 0.8075978452881823 8.924090198706121 >50 0.03712721618616317 1.5877612305119753 >100 0.015588007702203019 1.7397997486142958 >500 6.630428860256401E-4 0.27816893510135166 >1k 5.801625252724336E-4 0.7777506008067849 >5k 8.288036075320478E-5 0.2973347429633018 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5616 0.2975022209367188 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4051 0.21459784491001566 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2594 0.13741466543978784 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2592 0.13730871735540867 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.297404218958668E-5 0.0 10-11 0.0 0.0 0.0 5.297404218958668E-5 0.0 12-13 0.0 0.0 0.0 5.297404218958668E-5 0.0 14-15 0.0 0.0 0.0 5.297404218958668E-5 0.0 16-17 0.0 0.0 0.0 5.297404218958668E-5 0.0 18-19 5.297404218958668E-5 0.0 0.0 1.0594808437917336E-4 0.0 20-21 5.297404218958668E-5 0.0 0.0 1.324351054739667E-4 0.0 22-23 5.297404218958668E-5 0.0 0.0 2.648702109479334E-4 0.0 24-25 1.0594808437917336E-4 0.0 0.0 4.2379233751669345E-4 0.0 26-27 1.0594808437917336E-4 0.0 0.0 7.681236117490068E-4 0.0 28-29 1.324351054739667E-4 0.0 0.0 0.0018011174344459472 0.0 30-31 1.5892212656876004E-4 0.0 0.0 0.0029400593415220607 0.0 32-33 1.5892212656876004E-4 0.0 0.0 0.004052514227503381 0.0 34-35 1.5892212656876004E-4 0.0 0.0 0.005456326345527428 0.0 36-37 1.5892212656876004E-4 5.297404218958668E-5 0.0 0.007416365906542135 0.0 38-39 1.5892212656876004E-4 5.297404218958668E-5 0.0 0.009111535256608908 0.0 40-41 1.5892212656876004E-4 5.297404218958668E-5 0.0 0.012687283104406009 0.0 42-43 1.5892212656876004E-4 5.297404218958668E-5 0.0 0.017401972859279226 0.0 44-45 1.5892212656876004E-4 5.297404218958668E-5 0.0 0.026301611947129787 0.0 46-47 2.1189616875834673E-4 5.297404218958668E-5 0.0 0.044921987776769506 0.0 48-49 2.1189616875834673E-4 5.297404218958668E-5 0.0 0.060602304264887163 0.0 50-51 2.1189616875834673E-4 5.297404218958668E-5 0.0 0.07699777032256425 0.0 52-53 2.1189616875834673E-4 5.297404218958668E-5 0.0 0.09527381487797165 0.0 54-55 2.1189616875834673E-4 5.297404218958668E-5 0.0 0.11969484832737111 0.0 56-57 2.1189616875834673E-4 5.297404218958668E-5 0.0 0.14570510304245815 0.0 58-59 2.1189616875834673E-4 5.297404218958668E-5 0.0 0.17807224282029563 0.0 60-61 2.1189616875834673E-4 5.297404218958668E-5 0.0 0.21862387211642423 0.0 62-63 2.1189616875834673E-4 5.297404218958668E-5 0.0 0.2683929847535409 0.0 64-65 2.1189616875834673E-4 5.297404218958668E-5 0.0 0.3030644953666254 0.0 66-67 3.178442531375201E-4 5.297404218958668E-5 0.0 0.3654679170659585 0.0 68-69 3.178442531375201E-4 1.0594808437917336E-4 0.0 0.44614738332069903 0.0 70-71 3.178442531375201E-4 1.0594808437917336E-4 0.0 0.4962078531898584 0.0 72-73 3.178442531375201E-4 1.0594808437917336E-4 0.0 0.5879853812833173 0.0 74-75 3.178442531375201E-4 1.0594808437917336E-4 0.0 0.6623344494964023 0.0 76-77 3.178442531375201E-4 1.0594808437917336E-4 0.0 0.7221686301495405 0.0 78-79 3.178442531375201E-4 1.0594808437917336E-4 0.0 0.8027421483199018 0.0 80-81 3.7081829532710676E-4 1.0594808437917336E-4 0.0 0.8897520126162979 0.0 82-83 3.7081829532710676E-4 1.0594808437917336E-4 0.0 0.9958060450798505 0.0 84-85 3.7081829532710676E-4 1.0594808437917336E-4 0.0 1.1603963941628963 0.0 86-87 3.7081829532710676E-4 1.0594808437917336E-4 0.0 1.4002893442184394 0.0 88-89 3.7081829532710676E-4 1.0594808437917336E-4 0.0 1.6862432239578284 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9825 0.0 28.009254 1 GGTATCA 7080 0.0 25.979647 1 ATCAACG 11490 0.0 23.661541 3 TCAACGC 11495 0.0 23.609901 4 CAACGCA 11555 0.0 23.571447 5 GTACATG 7145 0.0 23.548138 1 TATCAAC 11590 0.0 23.498398 2 AACGCAG 11910 0.0 22.827734 6 TACATGG 7245 0.0 22.633293 2 GAGTACT 5795 0.0 22.349243 12-13 ACATGGG 7260 0.0 21.735443 3 GTACTTT 5995 0.0 21.246325 14-15 ACGCAGA 13060 0.0 20.745398 7 CGCAGAG 13105 0.0 20.601622 8 AGAGTAC 9475 0.0 20.290596 10-11 CATGGGG 3690 0.0 19.965141 4 AGTACTT 6000 0.0 19.684525 12-13 GCAGAGT 13635 0.0 19.033892 9 ATGGGGA 2080 0.0 17.139555 5 GTCTAGG 750 0.0 17.110315 1 >>END_MODULE