FastQCFastQC Report
Thu 26 May 2016
SRR936986_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936986_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887717
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57670.3055013013073464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47910.2537986361303098No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33540.17767493750387373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29120.1542604108560764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83000.025.707541
GTATCAA109300.024.4347781
GTACATG76800.023.6371141
TACATGG76250.023.4847892
TACGGTA1050.002199020522.6150117
ACATGGG76550.022.2746733
GAGTACT62250.021.32519712-13
ATCAACG127650.020.7995113
TCAACGC128500.020.6227784
CAACGCA129050.020.5716865
TATCAAC129100.020.5669862
AACGCAG131750.020.1501056
GTACTTT66200.019.7317514-15
AGAGTAC103750.019.31703410-11
AGTACTT65550.018.80248612-13
CATGGGG39350.018.5862664
ACGCAGA146400.018.0688387
CGCAGAG147350.017.8556528
CATGGGA41850.017.8164124
TAGACCG1700.001790273716.7617155
Produced by FastQC (version 0.11.5)