##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936986_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1887717 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26814612571694 33.0 31.0 34.0 31.0 34.0 2 32.346311973669785 33.0 31.0 34.0 31.0 34.0 3 32.486428315261236 34.0 31.0 34.0 31.0 34.0 4 36.13831363493575 37.0 35.0 37.0 35.0 37.0 5 36.0541113948754 37.0 35.0 37.0 35.0 37.0 6 36.084692779691025 37.0 35.0 37.0 35.0 37.0 7 36.04449501699672 37.0 35.0 37.0 35.0 37.0 8 36.02068424451335 37.0 35.0 37.0 35.0 37.0 9 37.726783728705094 39.0 37.0 39.0 35.0 39.0 10-11 37.63161506730087 39.0 37.0 39.0 35.0 39.0 12-13 37.56831744376938 39.0 37.0 39.0 35.0 39.0 14-15 38.826570402237195 40.0 38.0 41.0 35.0 41.0 16-17 38.7470847060232 40.0 38.0 41.0 35.0 41.0 18-19 38.767748290660094 40.0 38.0 41.0 34.5 41.0 20-21 38.85169996350089 40.0 38.0 41.0 35.0 41.0 22-23 38.787069777938115 40.0 38.0 41.0 35.0 41.0 24-25 38.709492736464206 40.0 38.0 41.0 34.0 41.0 26-27 38.543778807946325 40.0 38.0 41.0 34.0 41.0 28-29 38.39964835830794 40.0 38.0 41.0 34.0 41.0 30-31 38.39550022593429 40.0 38.0 41.0 34.0 41.0 32-33 38.341301953629696 40.0 38.0 41.0 34.0 41.0 34-35 38.24195205107546 40.0 38.0 41.0 34.0 41.0 36-37 38.095036226298745 40.0 38.0 41.0 33.5 41.0 38-39 37.966108002417734 40.0 37.5 41.0 33.0 41.0 40-41 37.764838691392825 40.0 37.0 41.0 33.0 41.0 42-43 37.56947863477417 40.0 37.0 41.0 33.0 41.0 44-45 37.35430919994893 40.0 37.0 41.0 32.5 41.0 46-47 37.05000643634612 40.0 37.0 41.0 31.5 41.0 48-49 36.725126965535615 39.5 36.0 41.0 31.0 41.0 50-51 36.50136884925018 39.0 36.0 41.0 30.0 41.0 52-53 36.29512448105304 39.0 35.5 40.5 30.0 41.0 54-55 36.03378472514683 39.0 35.0 40.0 29.5 41.0 56-57 35.78256539513073 38.5 35.0 40.0 29.0 41.0 58-59 35.45752223452986 38.0 35.0 40.0 28.0 41.0 60-61 35.12713346333163 38.0 34.0 40.0 28.0 41.0 62-63 34.801130148216075 37.5 34.0 40.0 27.5 41.0 64-65 34.399639617590985 37.0 34.0 39.5 26.5 41.0 66-67 33.973566482687815 36.0 33.0 39.0 26.0 40.5 68-69 33.534634693653764 36.0 33.0 39.0 26.0 40.0 70-71 33.1392753786717 35.0 32.0 38.0 25.0 40.0 72-73 32.75079792151048 35.0 32.0 37.5 24.5 40.0 74-75 32.285010941788414 35.0 32.0 37.0 23.5 39.0 76-77 30.80359185195662 33.5 29.5 35.5 21.5 38.0 78-79 31.51464732266542 34.0 31.0 36.0 22.5 38.5 80-81 31.227112962377305 34.0 31.0 36.0 20.0 37.5 82-83 31.037588791116463 34.0 31.0 35.0 20.5 37.0 84-85 30.714796497568226 34.0 31.0 35.0 20.0 36.5 86-87 30.36753072626882 34.0 31.0 35.0 18.5 36.0 88-89 30.00288999887165 34.0 30.0 35.0 17.0 36.0 90-91 29.582708372070602 34.0 30.0 35.0 9.0 35.0 92-93 29.177165327218013 34.0 29.0 35.0 4.5 35.0 94-95 28.715189300091062 34.0 29.0 35.0 2.0 35.0 96-97 28.124043222580504 33.5 29.0 35.0 2.0 35.0 98-99 27.389486347794715 33.0 27.5 35.0 2.0 35.0 100-101 24.22492857774762 30.0 20.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24864020595585856 1101 2 0.1440415367502439 1101 3 0.165008911522861 1101 4 0.11549951056210261 1101 5 0.1244939967404406 1101 6 0.1892764035614931 1101 7 0.15258772868855175 1101 8 0.19690762724970057 1101 9 0.11824293564271215 1101 10-11 0.2392027270314827 1101 12-13 0.18781615289806552 1101 14-15 0.2164662265663182 1101 16-17 0.25903727265126264 1101 18-19 0.24077457636659716 1101 20-21 0.22574310845369183 1101 22-23 0.2753663252670506 1101 24-25 0.2927305607049959 1101 26-27 0.22593308192535488 1101 28-29 0.22190614869015235 1101 30-31 0.1360902221746798 1101 32-33 0.22163200877786693 1101 34-35 0.2730089342478905 1101 36-37 0.2386304964526289 1101 38-39 0.2536534710636644 1101 40-41 0.3149988693984227 1101 42-43 0.2500804148252058 1101 44-45 0.30655484407126465 1101 46-47 0.31535497476595253 1101 48-49 0.3060335839217103 1101 50-51 0.30460335072298506 1101 52-53 0.20916368687885978 1101 54-55 0.1693514023839242 1101 56-57 0.282766085699258 1101 58-59 0.2642502203110979 1101 60-61 0.21954869083988626 1101 62-63 0.16516205621309865 1101 64-65 0.2547005650385188 1101 66-67 0.2353664512464846 1101 68-69 0.188918709820193 1101 70-71 0.1847928686682394 1101 72-73 0.4068498818727804 1101 74-75 0.2712088120838274 1101 76-77 0.23884558112068888 1101 78-79 0.38470323165360654 1101 80-81 0.7204213188423338 1101 82-83 0.5545537118317654 1101 84-85 0.409705151847799 1101 86-87 0.23840041055265004 1101 88-89 0.1774914631450102 1101 90-91 0.13162489955067613 1101 92-93 0.3260610960084911 1101 94-95 0.32402153570501824 1101 96-97 0.4303850409980505 1101 98-99 0.5557093150207422 1101 100-101 0.8580432673394327 1102 1 0.18514265893095683 1102 2 0.19297277862901296 1102 3 0.06957459063738014 1102 4 0.08396049581372012 1102 5 0.10158811066903439 1102 6 0.20310177738635105 1102 7 0.16131896110049837 1102 8 0.15365685883406854 1102 9 0.24946221619496356 1102 10-11 0.15061696299043348 1102 12-13 0.17109404680555684 1102 14-15 0.12251205055370917 1102 16-17 0.2641545085231769 1102 18-19 0.21845235025924126 1102 20-21 0.08412750408124481 1102 22-23 0.22699622148322618 1102 24-25 0.2127901405919701 1102 26-27 0.25784828823331196 1102 28-29 0.22708980498963882 1102 30-31 0.38865828784387446 1102 32-33 0.1983705443830317 1102 34-35 0.2603516438043414 1102 36-37 0.26228857477987333 1102 38-39 0.26912923982284553 1102 40-41 0.4662154702299759 1102 42-43 0.27966479854289616 1102 44-45 0.2677941347316377 1102 46-47 0.3696311524306708 1102 48-49 0.48712200859712596 1102 50-51 0.5169901115285214 1102 52-53 0.4877311509469351 1102 54-55 0.5060344502112173 1102 56-57 0.5262599376107246 1102 58-59 0.488639801312722 1102 60-61 0.5207441440180673 1102 62-63 0.359741462215986 1102 64-65 0.4132550962095536 1102 66-67 0.28626453940700003 1102 68-69 0.3099645948838443 1102 70-71 0.30451740712261 1102 72-73 0.3503938544861853 1102 74-75 0.2743626372133505 1102 76-77 0.31212716250708894 1102 78-79 0.327716031709798 1102 80-81 0.4350119781452406 1102 82-83 0.4257417900521254 1102 84-85 0.27237652800271306 1102 86-87 0.2948186470063163 1102 88-89 0.36027475390310215 1102 90-91 0.4007791766444804 1102 92-93 0.41024593413350985 1102 94-95 0.5024029462305926 1102 96-97 0.6235871774454615 1102 98-99 0.6753340319704719 1102 100-101 0.9355956058729014 1103 1 0.19388554942152325 1103 2 0.07080593338410068 1103 3 0.08896800006818495 1103 4 0.10085406005403286 1103 5 0.12229355414667253 1103 6 0.08138828944366594 1103 7 0.10751167923648097 1103 8 0.04254697785912498 1103 9 0.09668793596536318 1103 10-11 -0.0012450670219692483 1103 12-13 0.1049904801821171 1103 14-15 0.1736265851963239 1103 16-17 0.12552531388916321 1103 18-19 0.11320280671273508 1103 20-21 0.17239594564674832 1103 22-23 0.11768944908322965 1103 24-25 0.13332050858824118 1103 26-27 0.1341202125436567 1103 28-29 0.09313688959819899 1103 30-31 0.069186347771101 1103 32-33 -0.0016171646801694806 1103 34-35 0.14030672089948126 1103 36-37 0.16333518088630683 1103 38-39 0.12230912096667623 1103 40-41 0.12834700014198575 1103 42-43 -0.00590019987148338 1103 44-45 0.21353509057245645 1103 46-47 0.3324891611487928 1103 48-49 0.25616245234685664 1103 50-51 0.1847854177041981 1103 52-53 0.35425805121020915 1103 54-55 0.3962700046075298 1103 56-57 0.3043791615741043 1103 58-59 0.3222706191009834 1103 60-61 0.35908558061278484 1103 62-63 0.4611157015383469 1103 64-65 0.3284538247074522 1103 66-67 0.28255915034724666 1103 68-69 0.27076382477689975 1103 70-71 0.2079716294816123 1103 72-73 0.2853843688853459 1103 74-75 0.4241851223094102 1103 76-77 0.485407697979106 1103 78-79 0.47119273428601716 1103 80-81 0.7258129156340978 1103 82-83 0.5915785213760003 1103 84-85 0.37031174444517134 1103 86-87 0.36034951226950085 1103 88-89 0.2820921987768443 1103 90-91 0.18049734827850372 1103 92-93 0.24363787615781618 1103 94-95 0.4067217562427352 1103 96-97 0.7208286596386166 1103 98-99 0.7615685580563465 1103 100-101 0.8951008159165639 1104 1 0.09029531032486204 1104 2 0.06031277283694436 1104 3 0.05905818232729132 1104 4 0.05847502932385851 1104 5 -0.02319354372760074 1104 6 -0.011646267791753928 1104 7 -0.008707865305218832 1104 8 0.01451469412603501 1104 9 0.0610612071562997 1104 10-11 0.0565492568005439 1104 12-13 0.05667618743079572 1104 14-15 0.007641495582440427 1104 16-17 0.0077403682637608995 1104 18-19 0.013370003863286684 1104 20-21 0.004116986910823073 1104 22-23 0.025933263262309936 1104 24-25 0.03132551029337094 1104 26-27 0.16383583529383117 1104 28-29 0.07147988531643534 1104 30-31 0.1024978933737728 1104 32-33 -0.024086472398707315 1104 34-35 -0.08160557874813179 1104 36-37 0.042001677040303775 1104 38-39 0.14758904594109623 1104 40-41 0.1912202334412143 1104 42-43 0.11120411599377178 1104 44-45 0.28725814009692385 1104 46-47 0.17618113947473546 1104 48-49 0.15167228524443743 1104 50-51 0.12872037659169422 1104 52-53 0.07043307299793611 1104 54-55 0.1675831416618223 1104 56-57 0.09000446944421725 1104 58-59 0.10123030232632857 1104 60-61 0.06420806555082947 1104 62-63 0.11444827703757454 1104 64-65 0.12097873850382257 1104 66-67 -0.07476057986381335 1104 68-69 0.06647590465261288 1104 70-71 -0.004926707072520742 1104 72-73 0.12225394563499492 1104 74-75 -0.007316147062724099 1104 76-77 0.055306053479089456 1104 78-79 0.23232910653413086 1104 80-81 0.340950178337291 1104 82-83 0.18581045650540062 1104 84-85 0.008464902718824163 1104 86-87 0.13569290585064664 1104 88-89 0.08582595270810955 1104 90-91 0.12456303354999321 1104 92-93 0.0994277287590819 1104 94-95 0.09555554427653234 1104 96-97 0.2959721744732171 1104 98-99 0.6549924525658355 1104 100-101 0.7107103039996261 1105 1 0.11002090636625184 1105 2 0.08744952497369951 1105 3 0.046710004189634446 1105 4 0.056327031123231563 1105 5 0.09914113913340117 1105 6 0.06495067327886517 1105 7 0.11749546352968565 1105 8 0.0684058322276968 1105 9 0.08032571457869864 1105 10-11 0.09467341334575252 1105 12-13 0.1411397501838323 1105 14-15 0.19671594452530883 1105 16-17 0.22144369709866396 1105 18-19 0.1014435531210438 1105 20-21 0.12906075659143568 1105 22-23 -0.06775207187039456 1105 24-25 0.11911228492225234 1105 26-27 0.08100857483500334 1105 28-29 0.0877416936835047 1105 30-31 0.03249114573544176 1105 32-33 0.03164339714695075 1105 34-35 0.09004871724827268 1105 36-37 -0.001177585217902788 1105 38-39 0.17738549218491073 1105 40-41 0.15231447545651378 1105 42-43 0.20220726489166196 1105 44-45 0.2784749642084847 1105 46-47 0.26809521954671567 1105 48-49 0.3117712506065615 1105 50-51 0.24451547330784962 1105 52-53 0.1306332529349632 1105 54-55 0.09379771656061564 1105 56-57 0.05984814915631631 1105 58-59 0.34998198923590706 1105 60-61 0.17527981543837257 1105 62-63 0.34265340524270016 1105 64-65 0.21426483836886234 1105 66-67 0.1603833697538235 1105 68-69 0.2680334844996608 1105 70-71 0.18391719756085934 1105 72-73 0.2212980302053964 1105 74-75 0.23814044313071037 1105 76-77 0.17681415064507888 1105 78-79 0.2345558271819037 1105 80-81 0.41832872084741624 1105 82-83 0.26940244930791835 1105 84-85 0.11251348571477493 1105 86-87 -0.027549334365275513 1105 88-89 0.051351143891196216 1105 90-91 0.03556730705426858 1105 92-93 0.08734945615449519 1105 94-95 0.10841541832061807 1105 96-97 0.2601140997993525 1105 98-99 0.3171382015536892 1105 100-101 0.5737107538421782 1106 1 0.04048863109234446 1106 2 -0.009039844242941797 1106 3 -0.006750623433667613 1106 4 0.013060356277605933 1106 5 0.04131034685284618 1106 6 0.07864298840659245 1106 7 0.036642770236802846 1106 8 -0.0055858326730557906 1106 9 0.19935925944956523 1106 10-11 0.11203231706829087 1106 12-13 -0.001358102840335107 1106 14-15 0.18384431323262618 1106 16-17 0.12356669371926898 1106 18-19 0.17055851577667624 1106 20-21 -0.013680606245621618 1106 22-23 0.10942548210141467 1106 24-25 -0.08027856877481554 1106 26-27 0.039754108939384025 1106 28-29 0.040345575713224946 1106 30-31 0.028981630264205194 1106 32-33 0.010812155023835146 1106 34-35 -0.01578046154741486 1106 36-37 0.031415552638392796 1106 38-39 0.035682299046307264 1106 40-41 0.1564931781998098 1106 42-43 0.002780163550681891 1106 44-45 0.020128711843092617 1106 46-47 0.21491899651402235 1106 48-49 0.16123199719649506 1106 50-51 -0.13969282238370084 1106 52-53 0.025849679251869873 1106 54-55 0.21481744643201495 1106 56-57 0.10274491214877202 1106 58-59 0.14661597725746134 1106 60-61 0.07966224852893333 1106 62-63 0.14604897299233244 1106 64-65 0.1509132510938329 1106 66-67 0.13111757972248483 1106 68-69 0.26623969083984633 1106 70-71 0.29979998569245225 1106 72-73 0.362441708430417 1106 74-75 0.34301974777078215 1106 76-77 -0.002537332534153336 1106 78-79 0.11409654165084859 1106 80-81 0.5555948306015637 1106 82-83 0.3448421611112664 1106 84-85 0.28988436045407084 1106 86-87 0.31036607292812235 1106 88-89 0.09837549115477628 1106 90-91 0.305741444453286 1106 92-93 0.36955967018289115 1106 94-95 0.4331171652711703 1106 96-97 0.3902347765707468 1106 98-99 0.45196456604512747 1106 100-101 0.7180154125009075 1107 1 0.03259332351682076 1107 2 -0.0038070687954601112 1107 3 -0.054339347587038844 1107 4 0.010471655196447216 1107 5 0.05543284178849461 1107 6 0.02436332562989918 1107 7 0.06277434639122248 1107 8 0.040953106282508145 1107 9 -0.02228154327849552 1107 10-11 -0.054065619815382604 1107 12-13 0.023006142629434123 1107 14-15 0.051604833003196404 1107 16-17 0.10146006322981549 1107 18-19 0.12981877483136373 1107 20-21 0.0814714626387385 1107 22-23 0.07253879891505477 1107 24-25 0.08285228291244806 1107 26-27 0.03270670479285087 1107 28-29 0.027417067238800996 1107 30-31 0.08491095549457839 1107 32-33 0.09128914973107527 1107 34-35 0.1898470792788487 1107 36-37 0.15483568359621103 1107 38-39 0.2182422521635985 1107 40-41 0.30982872646454496 1107 42-43 0.21661794751180707 1107 44-45 0.17192649783456915 1107 46-47 0.21445524525082504 1107 48-49 0.2778365996393788 1107 50-51 0.2294716408876596 1107 52-53 0.16611943943939877 1107 54-55 0.2538936816023636 1107 56-57 0.1520778947846324 1107 58-59 0.2799686912405974 1107 60-61 0.2099971184709375 1107 62-63 0.20188947024443138 1107 64-65 0.18201457959123957 1107 66-67 0.06991394908075677 1107 68-69 0.17821231797921655 1107 70-71 0.008259639332557356 1107 72-73 0.06319409294250278 1107 74-75 0.06463171024615377 1107 76-77 0.19126685830064716 1107 78-79 0.31468626713778747 1107 80-81 0.3050941638149176 1107 82-83 0.3067707203445309 1107 84-85 0.1968814671999226 1107 86-87 0.19146809359158823 1107 88-89 -0.00966355624013815 1107 90-91 0.03275460945183184 1107 92-93 0.3470746555781865 1107 94-95 0.3260217709441626 1107 96-97 0.32858469881742636 1107 98-99 0.48515162161683634 1107 100-101 0.5379161097428558 1108 1 0.09124094272174688 1108 2 0.011652766493781996 1108 3 -0.043985702360643586 1108 4 0.03614082797844276 1108 5 0.01585948847230867 1108 6 0.02420054476770872 1108 7 0.056392206472700934 1108 8 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-0.9068457645926422 2316 92-93 -0.762909143303677 2316 94-95 -0.8023406436318687 2316 96-97 -0.9276116316268777 2316 98-99 -1.070618399537743 2316 100-101 -1.4235067755188098 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 8.0 5 23.0 6 54.0 7 156.0 8 426.0 9 777.0 10 1325.0 11 2292.0 12 3350.0 13 4775.0 14 5999.0 15 8318.0 16 10896.0 17 15132.0 18 14002.0 19 8993.0 20 7987.0 21 8131.0 22 8687.0 23 9349.0 24 11127.0 25 13334.0 26 16553.0 27 20784.0 28 25896.0 29 31967.0 30 41499.0 31 52721.0 32 67846.0 33 90333.0 34 125023.0 35 180857.0 36 286284.0 37 441350.0 38 342688.0 39 28771.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.846893438214515 19.553416012484004 15.071361768531938 22.528328780769545 2 15.528418888885945 20.859890917663762 39.81017872232214 23.801511471128148 3 19.60939619218771 24.80709811135223 29.91436236945612 25.669143327003933 4 13.38194589755339 17.325284801996062 37.05951375075157 32.233255549698974 5 13.921792066047574 37.61981427297708 34.10950043149826 14.348893229477081 6 31.70581183514266 36.57571553363137 17.416964513218876 14.301508118007094 7 28.26329370345237 32.86435413782892 20.85005326539942 18.022298893319284 8 26.33458970236503 34.92068453129843 19.733540285477417 19.011185480859133 9 26.16480771726664 17.031861271431335 20.50055225497493 36.30277875632709 10-11 24.786580827528702 26.87807547423687 27.395817275576796 20.939526422657632 12-13 25.910611506503894 24.144719939185734 27.878175466105848 22.066493088204524 14-15 22.846750863609323 25.620604147761554 26.322112901457157 25.210532087171966 16-17 22.060045049149345 28.809683236249523 26.846570140847458 22.283701573753678 18-19 22.03722505485063 27.546895532564307 29.051933737950808 21.36394567463426 20-21 22.951672478435974 26.491443918964556 29.117825069496224 21.439058533103246 22-23 22.610989888844568 26.4129633838123 29.031470289243565 21.944576438099567 24-25 22.50958684073832 26.572309426691426 29.0882111194903 21.829892613079956 26-27 22.41409498390411 26.652454567151246 29.121974620065178 21.81147582887947 28-29 22.173649879143078 27.032243965431608 28.967676567001092 21.826429588424222 30-31 22.46195969976321 26.615002142163906 28.92506824587113 21.99796991220175 32-33 22.355720458736446 26.65922200850026 28.97687511886114 22.00818241390216 34-35 22.6153004639434 26.757309790490417 28.83023485892969 21.7971548866365 36-37 22.64293445924359 26.59091426872966 28.886851423185394 21.879299848841356 38-39 22.37966463203852 26.836112172882732 28.922579498439394 21.861643696639355 40-41 22.639139362935303 26.783042618615205 28.658507511395833 21.919310507053655 42-43 22.427243538888256 26.86223084936995 28.423885273298133 22.286640338443664 44-45 22.6855643506241 26.78500101182725 28.42032045787567 22.10911417967297 46-47 22.88131305840568 27.119097497489648 27.983039538651717 22.01654990545295 48-49 22.88199724476679 27.133219843082557 27.82525962417899 22.159523287971666 50-51 22.722857655211214 27.35712293436453 27.838304385012968 22.081715025411285 52-53 22.987583850396987 27.409428327362544 27.43729347804494 22.165694344195533 54-55 22.767424237006335 27.284901912639302 27.557140923551906 22.390532926802457 56-57 22.875149619630413 27.528104916900347 27.41855381419804 22.17819164927119 58-59 23.010232285136183 27.28450446217265 27.461836524471693 22.24342672821948 60-61 22.8606035756419 27.278003005747152 27.842679808467054 22.018713610143894 62-63 22.58580073178342 27.280969552109767 27.968228288456377 22.165001427650438 64-65 22.67450284612037 27.432261434612077 27.69916551417047 22.194070205097084 66-67 22.73552588816111 27.326281919355576 27.47190837134904 22.46628382113427 68-69 22.698511121888505 27.352622627631085 27.665358281906016 22.283507968574398 70-71 22.884928251337257 27.194298038122916 27.519414546593058 22.40135916394677 72-73 22.782397190365803 27.186145947654193 27.531787324593225 22.49966953738678 74-75 22.70802170880126 27.72401670567961 27.155576475474557 22.41238511004457 76-77 22.757721923348935 27.469148065446632 27.24037093839149 22.53275907281295 78-79 22.82089638034377 27.359009617679558 27.245685924895373 22.574408077081305 80-81 23.033818426366075 27.482030509396687 27.037948290352322 22.446202773884913 82-83 22.92865812084896 27.44967363819903 27.257183482559338 22.36448475839267 84-85 22.77989925843678 27.54887359044849 27.12944816752013 22.5417789835946 86-87 22.740431960934824 27.515379688798692 27.13868127478854 22.605507075477945 88-89 22.78507064189565 27.41965047227034 26.98170780159878 22.813571084235228 90-91 22.803400420880884 27.24084637053145 27.203684892919057 22.75206831566861 92-93 22.764850626286275 27.290931099239028 27.25954394598761 22.684674328487088 94-95 22.694960695576256 27.402062085044186 27.16648452270778 22.736492696671775 96-97 22.521685672504162 27.36967737885517 27.293606352036687 22.81503059660398 98-99 22.491945944127643 27.53153484360842 26.967704580170665 23.008814632093273 100-101 22.574932457487947 27.45547491656513 26.90544048312762 23.064152142819307 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 170.0 1 134.0 2 111.0 3 133.5 4 131.5 5 134.0 6 212.0 7 474.0 8 875.0 9 1145.5 10 1182.5 11 1184.5 12 1302.5 13 1484.5 14 1696.0 15 1863.5 16 2024.0 17 2187.0 18 2380.0 19 2592.0 20 2906.0 21 3382.5 22 4021.0 23 5066.5 24 6351.0 25 8109.5 26 10357.5 27 13032.5 28 15790.0 29 18493.5 30 21630.5 31 25411.0 32 29225.5 33 32586.5 34 36507.5 35 40306.0 36 44596.5 37 48659.5 38 51330.0 39 53841.0 40 56469.0 41 59015.0 42 60885.0 43 62964.5 44 66274.5 45 69096.5 46 71268.5 47 74643.0 48 79283.5 49 78822.5 50 73013.0 51 69198.0 52 67514.5 53 66234.5 54 64259.5 55 59832.5 56 53276.5 57 47113.5 58 42618.5 59 38232.0 60 32264.5 61 26182.5 62 20861.0 63 15873.0 64 11641.5 65 8335.0 66 5944.5 67 4545.5 68 3283.0 69 2124.5 70 1398.5 71 886.0 72 536.0 73 337.0 74 225.0 75 133.5 76 88.0 77 47.0 78 23.0 79 9.5 80 8.5 81 9.0 82 6.0 83 2.0 84 1.0 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02659296917917251 2 0.008052054412817176 3 0.01213105566141535 4 1.0594808437917336E-4 5 0.005721196556475362 6 0.0 7 0.0 8 1.0594808437917336E-4 9 1.0594808437917336E-4 10-11 0.0 12-13 1.0594808437917336E-4 14-15 0.0 16-17 5.297404218958668E-5 18-19 0.004794150818157595 20-21 0.0012978640336448737 22-23 0.0 24-25 0.005482813366622221 26-27 0.006542294210413954 28-29 0.008184489518291142 30-31 0.03212875658798432 32-33 5.297404218958668E-4 34-35 0.0050060469869159414 36-37 0.015680316488117657 38-39 0.007866645265153622 40-41 0.009800197805073536 42-43 0.007177982716688995 44-45 0.002277883814152227 46-47 0.0017216563711615672 48-49 0.001933552539919914 50-51 0.0037876440165554477 52-53 0.002887085299332474 54-55 0.0019865265821095005 56-57 7.151495695594202E-4 58-59 8.210976539385935E-4 60-61 0.0 62-63 0.0 64-65 0.007813671222964036 66-67 5.562274429906602E-4 68-69 0.001085967864886527 70-71 0.004555767628304454 72-73 0.011204009923097584 74-75 0.005059021029105528 76-77 0.003814131037650241 78-79 0.01078021758558089 80-81 1.8540914766355338E-4 82-83 0.004661715712683628 84-85 8.210976539385935E-4 86-87 0.0 88-89 0.0014302991391188405 90-91 5.032534008010735E-4 92-93 1.0594808437917336E-4 94-95 8.475846750333869E-4 96-97 3.973053164219001E-4 98-99 1.8540914766355338E-4 100-101 9.005587172229735E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1887717.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.385410105448926 #Duplication Level Percentage of deduplicated Percentage of total 1 78.09850732425917 48.72207408047315 2 12.96837793804202 16.18075152134415 3 4.054828888178638 7.5888648928933735 4 1.7396795644577536 4.341224923230629 5 0.8963150590705374 2.7958491271902575 6 0.5248614740297645 1.9646218983538373 7 0.34316890855757265 1.4986113167062431 8 0.25148152932248846 1.2551022672583136 9 0.18201583524792384 1.02196192748648 >10 0.883082672405763 9.5464253490537 >50 0.03860460166130139 1.639386112981802 >100 0.017550657949757586 1.9343261178710043 >500 9.322786106195712E-4 0.41510794610306356 >1k 5.085156057924907E-4 0.7907724419713916 >5k 8.47526009654151E-5 0.30492007708271035 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5767 0.3055013013073464 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4791 0.2537986361303098 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3354 0.17767493750387373 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2912 0.1542604108560764 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.297404218958668E-5 0.0 10-11 0.0 0.0 0.0 5.297404218958668E-5 0.0 12-13 0.0 0.0 0.0 1.0594808437917336E-4 0.0 14-15 0.0 0.0 0.0 1.0594808437917336E-4 0.0 16-17 0.0 0.0 0.0 1.324351054739667E-4 0.0 18-19 0.0 0.0 0.0 1.5892212656876004E-4 0.0 20-21 0.0 0.0 0.0 1.5892212656876004E-4 0.0 22-23 0.0 0.0 0.0 2.648702109479334E-4 0.0 24-25 0.0 0.0 0.0 4.2379233751669345E-4 0.0 26-27 0.0 0.0 0.0 7.681236117490068E-4 0.0 28-29 5.297404218958668E-5 0.0 0.0 0.0018011174344459472 0.0 30-31 1.0594808437917336E-4 0.0 0.0 0.002887085299332474 0.0 32-33 1.0594808437917336E-4 0.0 0.0 0.004052514227503381 0.0 34-35 1.0594808437917336E-4 0.0 0.0 0.005482813366622222 0.0 36-37 1.0594808437917336E-4 0.0 0.0 0.0072839308010681685 0.0 38-39 1.0594808437917336E-4 0.0 0.0 0.008926126108945356 2.648702109479334E-5 40-41 1.0594808437917336E-4 0.0 0.0 0.012687283104406009 5.297404218958668E-5 42-43 1.0594808437917336E-4 0.0 0.0 0.017243050732710465 5.297404218958668E-5 44-45 1.0594808437917336E-4 0.0 0.0 0.026116202799466234 5.297404218958668E-5 46-47 1.0594808437917336E-4 0.0 0.0 0.04486901373457992 5.297404218958668E-5 48-49 1.0594808437917336E-4 0.0 0.0 0.06028446001174964 7.946106328438002E-5 50-51 1.0594808437917336E-4 0.0 0.0 0.07667992606942672 1.0594808437917336E-4 52-53 1.0594808437917336E-4 0.0 0.0 0.09479704849826537 1.0594808437917336E-4 54-55 1.0594808437917336E-4 0.0 0.0 0.11937700407423359 1.0594808437917336E-4 56-57 1.0594808437917336E-4 0.0 0.0 0.1455461809158894 1.0594808437917336E-4 58-59 1.0594808437917336E-4 0.0 0.0 0.17801926877810603 1.0594808437917336E-4 60-61 1.0594808437917336E-4 0.0 0.0 0.21862387211642423 1.0594808437917336E-4 62-63 1.0594808437917336E-4 0.0 0.0 0.2687108290066784 1.0594808437917336E-4 64-65 1.0594808437917336E-4 0.0 0.0 0.30375315791509006 1.0594808437917336E-4 66-67 1.5892212656876004E-4 0.0 0.0 0.3667922681206982 1.0594808437917336E-4 68-69 1.5892212656876004E-4 0.0 0.0 0.44773660458638664 1.0594808437917336E-4 70-71 1.8540914766355338E-4 0.0 0.0 0.49716138594927095 1.0594808437917336E-4 72-73 2.1189616875834673E-4 0.0 0.0 0.5884886346841185 1.0594808437917336E-4 74-75 2.1189616875834673E-4 0.0 0.0 0.6622020143909283 1.0594808437917336E-4 76-77 2.1189616875834673E-4 0.0 0.0 0.7216918637698342 1.0594808437917336E-4 78-79 2.1189616875834673E-4 0.0 0.0 0.8017356415182997 1.0594808437917336E-4 80-81 2.1189616875834673E-4 0.0 0.0 0.8887455058146958 1.0594808437917336E-4 82-83 2.1189616875834673E-4 0.0 0.0 0.9956471229532816 1.0594808437917336E-4 84-85 2.3838318985314004E-4 0.0 0.0 1.160158010973043 1.0594808437917336E-4 86-87 2.648702109479334E-4 0.0 0.0 1.4003158312395343 1.324351054739667E-4 88-89 2.648702109479334E-4 0.0 0.0 1.6874351399070942 1.5892212656876004E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8300 0.0 25.70754 1 GTATCAA 10930 0.0 24.434778 1 GTACATG 7680 0.0 23.637114 1 TACATGG 7625 0.0 23.484789 2 TACGGTA 105 0.0021990205 22.615011 7 ACATGGG 7655 0.0 22.274673 3 GAGTACT 6225 0.0 21.325197 12-13 ATCAACG 12765 0.0 20.799511 3 TCAACGC 12850 0.0 20.622778 4 CAACGCA 12905 0.0 20.571686 5 TATCAAC 12910 0.0 20.566986 2 AACGCAG 13175 0.0 20.150105 6 GTACTTT 6620 0.0 19.73175 14-15 AGAGTAC 10375 0.0 19.317034 10-11 AGTACTT 6555 0.0 18.802486 12-13 CATGGGG 3935 0.0 18.586266 4 ACGCAGA 14640 0.0 18.068838 7 CGCAGAG 14735 0.0 17.855652 8 CATGGGA 4185 0.0 17.816412 4 TAGACCG 170 0.0017902737 16.761715 5 >>END_MODULE