FastQCFastQC Report
Thu 26 May 2016
SRR936985_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936985_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1992805
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58300.29255245746573294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44340.22250044535215438No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28100.1410072736670171No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26490.1329282092327147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA100400.029.4846881
GGTATCA70700.025.2703151
ATCAACG117950.024.8181043
TCAACGC118700.024.6212564
TATCAAC119700.024.4155642
CAACGCA119250.024.388155
GTACATG79300.024.3872831
AACGCAG122000.023.876776
TACATGG78850.023.8057162
ACATGGG78550.022.928673
TATACGT1553.894954E-521.461114
GAGTACT58150.021.2016612-13
ACGCAGA136500.021.1317
CGCAGAG136350.020.979478
AGAGTAC101000.020.81567610-11
TATACCG1404.8389914E-420.3661565
AGTACTT64450.019.5346312-13
GCAGAGT143350.019.389069
GTACTTT64350.018.89814414-15
CATGGGA44100.018.7497924
Produced by FastQC (version 0.11.5)