##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936985_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1992805 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.692128933839488 33.0 31.0 34.0 30.0 34.0 2 31.822254560782415 33.0 31.0 34.0 30.0 34.0 3 31.829241697004974 33.0 31.0 34.0 30.0 34.0 4 35.40626905291787 37.0 35.0 37.0 33.0 37.0 5 35.39617022237499 37.0 35.0 37.0 33.0 37.0 6 35.48329716153864 37.0 35.0 37.0 33.0 37.0 7 35.415133944364854 37.0 35.0 37.0 33.0 37.0 8 35.374036596656474 37.0 35.0 37.0 33.0 37.0 9 36.9686848437253 39.0 37.0 39.0 33.0 39.0 10-11 36.83624965814518 39.0 37.0 39.0 33.0 39.0 12-13 36.7415715536643 39.0 37.0 39.0 32.0 39.0 14-15 37.908350541071506 40.0 38.0 41.0 33.0 41.0 16-17 37.82315429758556 40.0 38.0 41.0 32.5 41.0 18-19 37.75771036303101 40.0 37.0 41.0 32.0 41.0 20-21 37.63329979601617 40.0 37.0 41.0 32.0 41.0 22-23 37.49776219951275 40.0 37.0 41.0 32.0 41.0 24-25 37.62829479050885 40.0 37.0 41.0 32.0 41.0 26-27 37.57743156003723 40.0 37.0 41.0 32.0 41.0 28-29 37.48963320545663 40.0 37.0 41.0 32.0 41.0 30-31 37.313608707324605 40.0 37.0 41.0 31.0 41.0 32-33 37.1187685197498 40.0 36.5 41.0 31.0 41.0 34-35 36.93256565494366 39.0 36.0 41.0 30.0 41.0 36-37 36.752876473112025 39.0 36.0 41.0 30.0 41.0 38-39 36.599446759718084 39.0 36.0 41.0 30.0 41.0 40-41 36.4121948710486 39.0 35.0 41.0 30.0 41.0 42-43 36.18994307019503 39.0 35.0 40.5 30.0 41.0 44-45 35.9378842887287 39.0 35.0 40.0 28.5 41.0 46-47 35.622369725085996 38.5 35.0 40.0 27.5 41.0 48-49 35.38989615140468 38.5 35.0 40.0 27.0 41.0 50-51 34.70249146303828 38.0 34.0 39.5 25.5 40.5 52-53 34.8967957226121 38.0 34.5 39.5 26.0 40.5 54-55 35.30600987050916 39.0 35.0 40.0 26.5 41.0 56-57 35.19823590366343 39.0 35.0 40.0 26.0 41.0 58-59 34.94355443708742 38.0 34.0 40.0 26.0 41.0 60-61 34.65623455380732 38.0 34.0 40.0 26.0 41.0 62-63 34.3176883839613 38.0 34.0 40.0 25.0 41.0 64-65 33.979211463239 37.0 33.5 40.0 23.5 41.0 66-67 33.591221669957676 36.5 33.0 39.5 23.0 41.0 68-69 33.18486906646661 36.0 33.0 39.0 22.0 41.0 70-71 32.7796051294532 35.5 32.0 39.0 21.5 40.5 72-73 32.36573573430416 35.0 32.0 38.0 20.5 40.0 74-75 31.90966727803272 35.0 31.5 37.0 19.5 39.5 76-77 31.46954418520628 35.0 31.0 37.0 18.0 39.0 78-79 31.03248335888358 35.0 31.0 36.0 15.5 39.0 80-81 30.583266300516108 34.5 30.5 36.0 11.0 37.5 82-83 30.153023752951242 34.0 30.0 35.0 7.5 37.0 84-85 29.74974847012126 34.0 30.0 35.0 6.0 37.0 86-87 29.392607906945237 34.0 29.0 35.0 2.0 36.0 88-89 29.027441972496057 34.0 29.0 35.0 2.0 36.0 90-91 28.633870348579013 34.0 29.0 35.0 2.0 35.5 92-93 28.24451614683825 34.0 29.0 35.0 2.0 35.0 94-95 27.811238932058078 34.0 27.5 35.0 2.0 35.0 96-97 27.26981992718806 34.0 26.5 35.0 2.0 35.0 98-99 26.595025855515217 33.0 25.0 35.0 2.0 35.0 100-101 24.03484485436357 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3380997436402673 1101 2 0.4362849151660875 1101 3 0.29677312476762197 1101 4 0.37608159901551375 1101 5 0.38373028214563476 1101 6 0.13494514774597377 1101 7 0.1173695564806323 1101 8 0.062322492175511 1101 9 0.2360873385238378 1101 10-11 0.315220180385559 1101 12-13 0.31292600923497815 1101 14-15 0.3184848916541938 1101 16-17 0.42668456219412576 1101 18-19 0.4666691302099508 1101 20-21 0.5276561582556596 1101 22-23 0.5983949986388666 1101 24-25 0.5217548888073367 1101 26-27 0.5212372082481167 1101 28-29 0.49708456088706754 1101 30-31 0.5392372501310305 1101 32-33 0.6145709529286947 1101 34-35 0.5315714975760457 1101 36-37 0.5432677276415845 1101 38-39 0.6602630836357406 1101 40-41 0.4882746587553086 1101 42-43 0.4519450837721024 1101 44-45 0.346979604051036 1101 46-47 0.5470970602684133 1101 48-49 0.6550166782705276 1101 50-51 0.6471817711949299 1101 52-53 0.6344943125794273 1101 54-55 0.6119160845948954 1101 56-57 0.6092936567613094 1101 58-59 0.5847376350246591 1101 60-61 0.781289444398034 1101 62-63 0.8004345806849145 1101 64-65 0.7036227864612599 1101 66-67 0.6810454335979585 1101 68-69 0.6999102318484276 1101 70-71 0.5829375423019272 1101 72-73 0.5598260165471132 1101 74-75 0.5647621636006406 1101 76-77 0.6339867075897132 1101 78-79 0.5664214627342439 1101 80-81 0.41759049218840616 1101 82-83 0.47926872856571734 1101 84-85 0.3783378743452026 1101 86-87 0.46283606642657915 1101 88-89 0.47690081981206234 1101 90-91 0.3777238395943776 1101 92-93 0.5354149580630398 1101 94-95 0.6606192207955139 1101 96-97 0.5302862179910584 1101 98-99 0.21896201487350098 1101 100-101 0.6554917007386969 1102 1 0.21945132581262072 1102 2 0.380956526274975 1102 3 0.14967255306609317 1102 4 0.12110758118280529 1102 5 0.1949846947854681 1102 6 0.1855603364396572 1102 7 0.1743154265025737 1102 8 0.16529542057865143 1102 9 0.15768671612399032 1102 10-11 0.3011500098595761 1102 12-13 0.24469483258784663 1102 14-15 0.26493994569376866 1102 16-17 0.35882524340554767 1102 18-19 0.29686100043758756 1102 20-21 0.40625898016169515 1102 22-23 0.36876697716208895 1102 24-25 -0.002733143460304177 1102 26-27 0.2358268688950247 1102 28-29 0.3593831921475328 1102 30-31 0.3950050499410622 1102 32-33 0.5575661870574109 1102 34-35 0.3168268881742691 1102 36-37 0.09633796347971924 1102 38-39 0.26273247646837916 1102 40-41 0.33344101415182337 1102 42-43 0.4804302767809929 1102 44-45 0.1622096301701461 1102 46-47 0.1792087819597583 1102 48-49 0.3050553388467563 1102 50-51 0.18737402256969204 1102 52-53 0.32994502479783705 1102 54-55 0.4696347256196134 1102 56-57 0.4662683905377065 1102 58-59 0.3673221227964447 1102 60-61 0.37398174317677757 1102 62-63 0.44244875361194147 1102 64-65 0.46591971309388924 1102 66-67 0.37769532276841744 1102 68-69 0.2645030814901048 1102 70-71 0.29107260092292364 1102 72-73 0.3054244855231758 1102 74-75 0.3187153200446744 1102 76-77 0.3697837580472729 1102 78-79 0.440091972730162 1102 80-81 0.3744185896647707 1102 82-83 0.5472467009434681 1102 84-85 0.48088091093091734 1102 86-87 0.5221279065646662 1102 88-89 0.4787010217242731 1102 90-91 0.47091938664679844 1102 92-93 0.2939000304072543 1102 94-95 0.4368701542707889 1102 96-97 0.40617571290600196 1102 98-99 0.32892755649217875 1102 100-101 0.18874831943509207 1103 1 0.058008034865565605 1103 2 0.21872422024702232 1103 3 0.2125164333681333 1103 4 0.33973704041336816 1103 5 0.34546475118003883 1103 6 0.2999732393347472 1103 7 0.12469008420112715 1103 8 0.13062928053668088 1103 9 0.2360946057585025 1103 10-11 0.28987625850498233 1103 12-13 0.44345020232503174 1103 14-15 0.4857981558668101 1103 16-17 0.44876540253987685 1103 18-19 0.4307320957671692 1103 20-21 0.5057950120040502 1103 22-23 0.5634505939157606 1103 24-25 0.30161665482362565 1103 26-27 0.4083665257075708 1103 28-29 0.3709656163802322 1103 30-31 0.5086266328502518 1103 32-33 0.6004753127663776 1103 34-35 0.4298558339088814 1103 36-37 0.5462926674865898 1103 38-39 0.5812138317230122 1103 40-41 0.6093135267213938 1103 42-43 0.6533054355658976 1103 44-45 0.6494527185437065 1103 46-47 0.7038903501138662 1103 48-49 0.7648609302512668 1103 50-51 0.64718708186642 1103 52-53 0.7473635975748465 1103 54-55 0.6849750854097536 1103 56-57 0.6901464872467287 1103 58-59 0.7038343811110224 1103 60-61 0.726941895794674 1103 62-63 0.7922843770066876 1103 64-65 0.792350224725638 1103 66-67 0.7043763314759772 1103 68-69 0.7980420307890199 1103 70-71 0.8429895719753446 1103 72-73 0.6793426783596388 1103 74-75 0.7413306238394597 1103 76-77 0.5700849814724123 1103 78-79 0.4623741347753061 1103 80-81 0.6473907941699579 1103 82-83 0.6869631275588866 1103 84-85 0.6944405011151211 1103 86-87 0.683170236237256 1103 88-89 0.5616792613927224 1103 90-91 0.7231667151236039 1103 92-93 0.6177448074095402 1103 94-95 0.7948889279986311 1103 96-97 0.637317118459201 1103 98-99 0.4921512482603667 1103 100-101 0.379379175622482 1104 1 0.24195848229014416 1104 2 0.3312367311392528 1104 3 0.31229009075718395 1104 4 0.3245356656315508 1104 5 0.3217524625837669 1104 6 0.27000483018068167 1104 7 0.23555749707001183 1104 8 0.27236076104397 1104 9 0.30361970173154873 1104 10-11 0.33958017862951095 1104 12-13 0.3185414542775433 1104 14-15 0.44487546576194603 1104 16-17 0.45804383092901446 1104 18-19 0.4191807990856873 1104 20-21 0.5469653570753721 1104 22-23 0.4556073773147844 1104 24-25 0.37772766252034984 1104 26-27 0.3137145780550341 1104 28-29 0.41568676174398433 1104 30-31 0.227582759073357 1104 32-33 0.4121633862103735 1104 34-35 0.3115784657419809 1104 36-37 0.25565256842460116 1104 38-39 0.285431519898971 1104 40-41 0.32306701021241224 1104 42-43 0.41072604704164917 1104 44-45 0.18307012669149714 1104 46-47 0.3480838123464878 1104 48-49 0.3296164991158008 1104 50-51 0.05723788080997849 1104 52-53 0.06050145074620161 1104 54-55 0.04846486222352553 1104 56-57 0.08993093570185096 1104 58-59 0.13634624453928978 1104 60-61 0.13010340837841028 1104 62-63 0.19155166362010334 1104 64-65 0.14193975954976423 1104 66-67 0.006484096457349153 1104 68-69 0.09979207192995432 1104 70-71 0.06423745085641741 1104 72-73 0.0884964699355919 1104 74-75 0.04567321860524842 1104 76-77 -0.04325466463457772 1104 78-79 -0.04875307300936882 1104 80-81 -0.03173011163212891 1104 82-83 -0.007162216069122707 1104 84-85 0.03791662723031308 1104 86-87 -0.04861376978415777 1104 88-89 0.02037348844214648 1104 90-91 -0.07707527453315777 1104 92-93 -0.009671784394104321 1104 94-95 0.26656345865470144 1104 96-97 0.037173867057038734 1104 98-99 -0.16719421962676861 1104 100-101 -0.12566510821493182 1105 1 0.20549235521683684 1105 2 0.2150600560356466 1105 3 0.3590706097681142 1105 4 0.3247628140382659 1105 5 0.3001362784915713 1105 6 0.2328795091597229 1105 7 0.1389248563075398 1105 8 0.17948024110589955 1105 9 0.24181030242318968 1105 10-11 0.2359638832809523 1105 12-13 0.3894933451721343 1105 14-15 0.32975897107907315 1105 16-17 0.379762463588321 1105 18-19 0.39183583946248035 1105 20-21 0.41513908388537146 1105 22-23 0.3771387284305945 1105 24-25 0.23918150503697433 1105 26-27 0.12483094293882147 1105 28-29 0.11769772049568417 1105 30-31 -0.14075043518953123 1105 32-33 0.016691056594858367 1105 34-35 -0.17618982434043318 1105 36-37 -0.05075811589474455 1105 38-39 -0.08034231445884643 1105 40-41 0.01332744281423004 1105 42-43 0.03820451429983507 1105 44-45 -0.021071391401562778 1105 46-47 0.06967403662076777 1105 48-49 0.03621863709223305 1105 50-51 0.03943918065514396 1105 52-53 0.05989742141160548 1105 54-55 0.11065253316429136 1105 56-57 0.13297463163821277 1105 58-59 0.21461648624373453 1105 60-61 0.013413563302897558 1105 62-63 0.12448864616212063 1105 64-65 0.2494185049488422 1105 66-67 0.17263898385664334 1105 68-69 0.3323299222715974 1105 70-71 0.29468182731086046 1105 72-73 0.2846599797029725 1105 74-75 0.237881101118532 1105 76-77 0.09650743217699898 1105 78-79 0.1803126516867657 1105 80-81 0.15583977127054993 1105 82-83 0.1916408508979366 1105 84-85 0.24192417412023204 1105 86-87 0.1223663987692305 1105 88-89 0.20694623619615982 1105 90-91 0.22309670926049918 1105 92-93 0.19083860416873932 1105 94-95 0.35055413166298166 1105 96-97 0.2661779371856312 1105 98-99 0.0846651523310662 1105 100-101 0.2811674231454582 1106 1 0.17083770240915896 1106 2 0.2964134603040556 1106 3 0.36238877201671116 1106 4 0.3825677220891919 1106 5 0.3171507012728014 1106 6 0.31741538159960214 1106 7 0.31037489936061746 1106 8 0.36839407344491093 1106 9 0.3153876288270254 1106 10-11 0.46734054389968804 1106 12-13 0.4898667921860991 1106 14-15 0.6629774962036237 1106 16-17 0.485972870132386 1106 18-19 0.34988599629869555 1106 20-21 0.6353136794939545 1106 22-23 0.5590199173106001 1106 24-25 0.6373331056889171 1106 26-27 0.512454336136436 1106 28-29 0.5135586667408489 1106 30-31 0.4299961513164092 1106 32-33 0.33582315266472307 1106 34-35 0.415122487302348 1106 36-37 0.4566718075322811 1106 38-39 0.5419684057576504 1106 40-41 0.42285235978329183 1106 42-43 0.1542277552740643 1106 44-45 0.45645394072218437 1106 46-47 0.6780855317926751 1106 48-49 0.44439756876084857 1106 50-51 0.34536762797662846 1106 52-53 0.5367656174876316 1106 54-55 0.4082834606506083 1106 56-57 0.5292985906762837 1106 58-59 0.42759641274597726 1106 60-61 0.5433398733465609 1106 62-63 0.5990938648102571 1106 64-65 0.5124366794983288 1106 66-67 0.5612041631419586 1106 68-69 0.49803468578718935 1106 70-71 0.49989605481562904 1106 72-73 0.41453112799007386 1106 74-75 0.4729683989228235 1106 76-77 0.530367909451126 1106 78-79 0.5283800681945472 1106 80-81 0.5813075890945214 1106 82-83 0.6307203607078868 1106 84-85 0.7544741464432541 1106 86-87 0.7257341489383684 1106 88-89 0.8311933839297225 1106 90-91 0.6750285239477449 1106 92-93 0.4215241091198436 1106 94-95 0.4836450351625814 1106 96-97 0.6166653595363307 1106 98-99 0.4994492718956174 1106 100-101 0.4988585865011359 1107 1 0.031114039329764864 1107 2 0.22772533768454295 1107 3 0.2883589662709234 1107 4 0.28443304245173806 1107 5 0.2794493402011753 1107 6 0.2689958506524519 1107 7 0.11949702508130144 1107 8 0.2736313072452674 1107 9 0.22353856041041098 1107 10-11 0.1822453204181258 1107 12-13 0.29560344836168184 1107 14-15 0.3283166257823211 1107 16-17 0.3466283934865828 1107 18-19 0.25251468894386164 1107 20-21 0.36991644132488233 1107 22-23 0.3816466178809961 1107 24-25 0.3218862387072079 1107 26-27 0.16902006845080564 1107 28-29 0.20968102397291943 1107 30-31 0.23042660098690249 1107 32-33 0.21747179836153663 1107 34-35 0.18956818672825904 1107 36-37 0.25708812423986416 1107 38-39 0.2558280160186044 1107 40-41 0.28282748192286533 1107 42-43 0.23661975636316868 1107 44-45 0.2158694744443963 1107 46-47 0.13198438361922626 1107 48-49 -0.007313654234607725 1107 50-51 -0.07691270924549087 1107 52-53 0.05045478613867971 1107 54-55 0.2510393461163076 1107 56-57 0.2691803506551196 1107 58-59 0.14913156786629855 1107 60-61 0.2301504347270722 1107 62-63 0.2873885795820712 1107 64-65 0.35587152403957845 1107 66-67 0.39051239018002804 1107 68-69 0.30916311538619823 1107 70-71 0.31501449865911724 1107 72-73 0.27073024932358436 1107 74-75 0.22941351072513783 1107 76-77 0.17363308152331314 1107 78-79 0.2371863270012078 1107 80-81 0.09607720833903954 1107 82-83 0.16272450330044919 1107 84-85 0.05906930147127909 1107 86-87 0.06512042838877008 1107 88-89 0.15962546340965744 1107 90-91 0.3001266855473155 1107 92-93 0.11630623844010657 1107 94-95 -0.12187724223267438 1107 96-97 -0.2058669289960875 1107 98-99 -0.14791119057338875 1107 100-101 -0.4015520001071984 1108 1 -0.021943987731685155 1108 2 0.14720571382517988 1108 3 -0.03549855192694906 1108 4 0.18054307420187143 1108 5 0.2740168984559048 1108 6 0.14796115624749007 1108 7 0.04452982893808155 1108 8 0.19849970724772703 1108 9 0.22251791051716907 1108 10-11 0.043925050941943766 1108 12-13 0.10848474993257895 1108 14-15 0.3157063694430633 1108 16-17 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2316 58-59 -1.4215099989801914 2316 60-61 -1.318506056886001 2316 62-63 -1.0165403474399852 2316 64-65 -1.1291003332759928 2316 66-67 -1.2288322764256776 2316 68-69 -0.9466979542390135 2316 70-71 -1.0941866097765711 2316 72-73 -1.7467569493109387 2316 74-75 -1.8649789070057707 2316 76-77 -1.953115549441769 2316 78-79 -1.9395766547009679 2316 80-81 -2.1743978645576227 2316 82-83 -1.7325022891447155 2316 84-85 -1.7782723324299852 2316 86-87 -1.771839958496944 2316 88-89 -1.7758048555068981 2316 90-91 -1.8438678484728221 2316 92-93 -2.3159134522951597 2316 94-95 -2.0642054397800997 2316 96-97 -2.1396931402998973 2316 98-99 -1.9800626835580353 2316 100-101 -1.9795960073409873 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3910.0 3 1502.0 4 1629.0 5 1765.0 6 1912.0 7 2284.0 8 2931.0 9 3709.0 10 4556.0 11 5825.0 12 7080.0 13 8486.0 14 9335.0 15 10962.0 16 13472.0 17 17468.0 18 15619.0 19 11174.0 20 11507.0 21 12377.0 22 13463.0 23 14928.0 24 17117.0 25 19338.0 26 22588.0 27 26929.0 28 32328.0 29 39052.0 30 47291.0 31 58592.0 32 74144.0 33 96291.0 34 130165.0 35 183794.0 36 274647.0 37 395470.0 38 341642.0 39 57482.0 40 41.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.56753750577852 18.78985671284294 14.724340245134627 22.918265536243915 2 15.127319409390907 20.09001363554013 40.839471501118894 23.943195453950068 3 19.2247275369825 24.680562330745712 29.795027913783446 26.29968221848834 4 12.674496167646817 16.752384962901136 37.824128842956085 32.74899002649596 5 13.809009056050915 37.228467841906465 34.35445072995174 14.608072372090874 6 31.439965542272464 36.09918845625967 17.996983944827473 14.463862056640393 7 27.911409653314408 32.63653596467147 21.257296748752946 18.19475763326118 8 25.899847630235982 34.67885624778788 20.103169642520953 19.318126479455184 9 25.9652595893512 17.086608086687395 20.725235937843518 36.222896386117895 10-11 24.523004307441504 26.60477542780794 27.788669350912432 21.08355091383812 12-13 25.65145335069187 23.98522466327678 28.077983899932914 22.285338086098438 14-15 22.739965012774526 25.342601024923443 26.491618449023473 25.42581551327856 16-17 21.953418037392346 28.513453049273352 27.029638892297562 22.50349002103674 18-19 21.910324251930447 27.331637455561005 29.125709512047433 21.63232878046112 20-21 22.951347458467115 26.28677609944054 29.012708605441023 21.749167836651324 22-23 22.450678694882388 26.28881267539475 28.976592955705172 22.283915674017692 24-25 22.31645456235656 26.39068392004193 29.082816652904413 22.210044864697096 26-27 22.158531937544268 26.44212035958387 29.130569070156692 22.268778632715165 28-29 22.0442778411322 26.78140717071696 29.02129335857474 22.153021629576102 30-31 22.266252844608477 26.459588369158045 28.94688642391002 22.32727236232346 32-33 22.131952694641576 26.466587799312276 28.997401893569215 22.404057612476933 34-35 22.493813363936784 26.57846094710291 28.78196470449796 22.145760984462342 36-37 22.456562483534515 26.382385632312243 28.90082070247716 22.26023118167608 38-39 22.208026688050293 26.657992113616906 28.902244176792273 22.231737021540525 40-41 22.44882085744509 26.56505607174614 28.71827836555928 22.267844705249487 42-43 22.193594197116877 26.588485311514816 28.518305251022113 22.699615240346198 44-45 22.40168747236932 26.574101905707685 28.49544084387669 22.5287697780463 46-47 22.588938706998427 26.79956142221642 28.165977102626698 22.445522768158447 48-49 22.496197559312304 26.83607328185131 28.101203382779698 22.566525776056693 50-51 22.415110776127896 27.036089367589273 28.080447490293825 22.468352365989013 52-53 22.603044419805666 27.128902216913094 27.66014140451582 22.607911958765424 54-55 22.474829653073623 26.9114396708303 27.77373120801858 22.839999468077497 56-57 22.568711857934314 27.140628759635018 27.656311118571313 22.634348263859355 58-59 22.732432249339492 26.9303512421059 27.699776945330278 22.637439563224333 60-61 22.571548435044996 26.945900144574736 28.072965829478093 22.40958559090218 62-63 22.35295386948447 26.913967017587176 28.151096043224495 22.581983069703863 64-65 22.411985281849077 27.103602885921784 27.88806075941745 22.59635107281169 66-67 22.40268555400592 27.074275803264804 27.626748543476392 22.89629009925288 68-69 22.349488879871902 27.01889125689354 27.884798037475367 22.746821825759188 70-71 22.5614017916411 26.818425368573877 27.795583764052488 22.824589075732536 72-73 22.397564843812738 26.84579017104312 27.80780735565727 22.94883762948687 74-75 22.339096844912177 27.356566556174478 27.46874009541526 22.83559650349808 76-77 22.496040180714832 27.014588649379746 27.47305177016659 23.016319399738833 78-79 22.480248784260663 26.99189774790216 27.55440775463271 22.97344571320447 80-81 22.551220655839263 27.150524543895067 27.3959158018817 22.90233899838397 82-83 22.57513151167586 27.079345231357394 27.541766361372677 22.803756895594073 84-85 22.5392197556046 27.073865190543994 27.41154381063898 22.975371243212425 86-87 22.466275305127 27.076266316559188 27.5323445406872 22.92511383762662 88-89 22.49060944448111 26.976395446963842 27.31457840301461 23.218416705540434 90-91 22.440439308487907 26.851944567205148 27.537087326402354 23.170528797904595 92-93 22.465333637853536 26.907296186888395 27.58453572799988 23.04283444725819 94-95 22.379766541651673 26.90196184709025 27.56721124221542 23.151060369042654 96-97 22.189629683908702 26.942254158392707 27.687217699446382 23.1808984582522 98-99 22.165739533517964 27.019290978427684 27.43743576680165 23.377533721252703 100-101 22.38718794395667 26.741715737212797 27.385932942776964 23.48516337605357 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 140.0 1 109.0 2 99.0 3 122.0 4 116.5 5 138.0 6 243.0 7 509.5 8 904.5 9 1196.0 10 1192.0 11 1178.5 12 1332.0 13 1463.5 14 1569.0 15 1705.0 16 1865.5 17 2009.0 18 2200.5 19 2458.0 20 2763.5 21 3241.5 22 3927.0 23 5027.0 24 6451.0 25 8214.0 26 10765.0 27 13950.5 28 16900.5 29 19569.5 30 22850.0 31 26845.0 32 30645.5 33 34255.0 34 38562.5 35 42561.5 36 46655.0 37 50881.5 38 54023.0 39 56544.0 40 59277.5 41 62252.5 42 64124.0 43 66624.0 44 70016.5 45 72896.5 46 75373.5 47 78376.0 48 82803.0 49 82836.5 50 77774.0 51 73676.5 52 71487.5 53 70127.5 54 68365.0 55 63350.5 56 56473.5 57 50672.0 58 45758.5 59 40534.5 60 34206.5 61 28034.0 62 22382.0 63 17052.5 64 12593.0 65 9159.5 66 6523.0 67 4815.0 68 3451.0 69 2304.5 70 1534.0 71 960.5 72 622.5 73 430.0 74 279.0 75 172.0 76 109.0 77 57.5 78 35.5 79 30.0 80 26.5 81 18.5 82 17.0 83 14.0 84 9.0 85 7.5 86 7.5 87 8.5 88 7.5 89 5.0 90 5.0 91 3.5 92 3.5 93 3.0 94 1.5 95 2.0 96 1.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025692428511570373 2 0.010939354327192074 3 0.012093506389235273 4 0.02137690341001754 5 0.05509821583145365 6 0.04009423902489205 7 0.015455601526491553 8 0.04716969297046124 9 0.027248024769106864 10-11 0.02202925022769413 12-13 0.03520163789231761 14-15 0.01824062063272623 16-17 0.01698610752180971 18-19 0.01638394122856978 20-21 0.01292148504244018 22-23 0.00787834233655576 24-25 0.0017814086175014616 26-27 7.527078665499134E-5 28-29 0.0023835749107413923 30-31 0.0 32-33 3.7635393327495667E-4 34-35 7.527078665499134E-5 36-37 0.0 38-39 1.5054157330998268E-4 40-41 5.0180524436660884E-5 42-43 1.2545131109165222E-4 44-45 1.0036104887332177E-4 46-47 0.0 48-49 3.0108314661996536E-4 50-51 1.0036104887332177E-4 52-53 5.770760310216002E-4 54-55 0.0016810475686281398 56-57 4.2653445771161753E-4 58-59 0.0010036104887332178 60-61 0.0032115535639462966 62-63 0.0023083041240864006 64-65 0.0013799644220081745 66-67 0.010939354327192074 68-69 0.013523651335680108 70-71 0.024964810907238795 72-73 0.016308670441914787 74-75 0.017889356961669604 76-77 0.03038430754639817 78-79 0.019871487676917713 80-81 0.04435958360200822 82-83 0.034198027403584394 84-85 0.018968238237057813 86-87 0.013749463695645081 88-89 0.012269138224763589 90-91 0.022405604160969084 92-93 0.03010831466199653 94-95 0.012244047962545256 96-97 0.01798971801054293 98-99 0.02772473975125514 100-101 0.03888990643841219 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1992805.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.66704116126658 #Duplication Level Percentage of deduplicated Percentage of total 1 78.96744382560928 50.27623496445073 2 12.53762416514401 15.964668675734284 3 3.8235481419212585 7.303019908013554 4 1.683012687169951 4.286097521159325 5 0.8534331527645362 2.7167781832724622 6 0.5142377819911251 1.9643998819624438 7 0.34339614998571777 1.5304111771032813 8 0.23876157177884064 1.2160994254537723 9 0.16962479603094832 0.9719557983786452 >10 0.8138105400777588 9.010377198440246 >50 0.03769902846000595 1.659168604210799 >100 0.016148700913427027 1.7735103259481317 >500 6.297290765534793E-4 0.26686894496753594 >1k 5.51012941984268E-4 0.7682317478698986 >5k 7.871613456918113E-5 0.2921776430348691 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5830 0.29255245746573294 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4434 0.22250044535215438 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2810 0.1410072736670171 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2649 0.1329282092327147 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.018052443666089E-5 0.0 10-11 0.0 0.0 0.0 5.018052443666089E-5 0.0 12-13 5.018052443666089E-5 0.0 0.0 5.018052443666089E-5 0.0 14-15 5.018052443666089E-5 0.0 0.0 1.0036104887332178E-4 0.0 16-17 5.018052443666089E-5 0.0 0.0 1.2545131109165222E-4 0.0 18-19 5.018052443666089E-5 0.0 0.0 1.5054157330998265E-4 0.0 20-21 5.018052443666089E-5 0.0 0.0 1.5054157330998265E-4 0.0 22-23 5.018052443666089E-5 0.0 0.0 2.0072209774664356E-4 0.0 24-25 5.018052443666089E-5 0.0 0.0 2.759928844016349E-4 0.0 26-27 5.018052443666089E-5 0.0 0.0 3.2617340883829576E-4 0.0 28-29 5.018052443666089E-5 0.0 0.0 7.025273421132524E-4 0.0 30-31 5.018052443666089E-5 0.0 0.0 0.001304693635353183 0.0 32-33 5.018052443666089E-5 0.0 0.0 0.0025592067462697054 0.0 34-35 7.527078665499134E-5 0.0 0.0 0.003688268546094575 0.0 36-37 1.0036104887332178E-4 0.0 0.0 0.0048926011325744366 0.0 38-39 1.0036104887332178E-4 0.0 0.0 0.0066991000122942285 0.0 40-41 1.0036104887332178E-4 0.0 0.0 0.010437549082825466 0.0 42-43 1.0036104887332178E-4 0.0 0.0 0.01472798392215997 0.0 44-45 1.0036104887332178E-4 0.0 0.0 0.023609936747448948 0.0 46-47 2.0072209774664356E-4 0.0 0.0 0.041474203446900226 0.0 48-49 2.0072209774664356E-4 0.0 0.0 0.054019334556065446 0.0 50-51 2.0072209774664356E-4 0.0 0.0 0.06965056791808531 0.0 52-53 2.0072209774664356E-4 0.0 0.0 0.08804173012412153 0.0 54-55 2.0072209774664356E-4 0.0 0.0 0.1108487784805839 0.0 56-57 2.0072209774664356E-4 0.0 0.0 0.13428308339250455 0.0 58-59 2.0072209774664356E-4 0.0 0.0 0.16489320329886767 0.0 60-61 2.0072209774664356E-4 0.0 0.0 0.20127408351544682 0.0 62-63 2.0072209774664356E-4 0.0 0.0 0.24751543678382984 0.0 64-65 2.0072209774664356E-4 0.0 0.0 0.2825916233650558 0.0 66-67 2.0072209774664356E-4 0.0 0.0 0.34075085118714576 0.0 68-69 2.0072209774664356E-4 0.0 0.0 0.4167492554464687 0.0 70-71 2.0072209774664356E-4 0.0 0.0 0.4678079390607711 0.0 72-73 2.25812359964974E-4 0.0 0.0 0.5575056264913025 0.0 74-75 2.5090262218330445E-4 0.0 0.0 0.6297404914178759 0.0 76-77 2.5090262218330445E-4 0.0 0.0 0.6882008023865858 0.0 78-79 2.5090262218330445E-4 0.0 0.0 0.7662315178855934 0.0 80-81 3.512636710566262E-4 0.0 0.0 0.8488286611083373 0.0 82-83 3.512636710566262E-4 0.0 0.0 0.9502685912570472 0.0 84-85 3.7635393327495667E-4 0.0 0.0 1.1021650387268198 0.0 86-87 4.2653445771161753E-4 0.0 0.0 1.323511332016931 0.0 88-89 4.51624719929948E-4 0.0 0.0 1.5937836366327864 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10040 0.0 29.484688 1 GGTATCA 7070 0.0 25.270315 1 ATCAACG 11795 0.0 24.818104 3 TCAACGC 11870 0.0 24.621256 4 TATCAAC 11970 0.0 24.415564 2 CAACGCA 11925 0.0 24.38815 5 GTACATG 7930 0.0 24.387283 1 AACGCAG 12200 0.0 23.87677 6 TACATGG 7885 0.0 23.805716 2 ACATGGG 7855 0.0 22.92867 3 TATACGT 155 3.894954E-5 21.46111 4 GAGTACT 5815 0.0 21.20166 12-13 ACGCAGA 13650 0.0 21.131 7 CGCAGAG 13635 0.0 20.97947 8 AGAGTAC 10100 0.0 20.815676 10-11 TATACCG 140 4.8389914E-4 20.366156 5 AGTACTT 6445 0.0 19.53463 12-13 GCAGAGT 14335 0.0 19.38906 9 GTACTTT 6435 0.0 18.898144 14-15 CATGGGA 4410 0.0 18.749792 4 >>END_MODULE