FastQCFastQC Report
Thu 26 May 2016
SRR936985_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936985_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1992805
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60570.303943436512855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49390.24784161019266812No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33290.1670509658496441No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29870.14988922649230607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA118750.025.7632181
GTACATG80000.024.0498811
TACATGG80900.023.0736142
GGTATCA89300.022.6092071
ACATGGG80450.022.433473
ATCAACG137100.022.1708033
TATCAAC137750.022.1023272
TCAACGC137750.022.100114
TATTCCG2154.4174158E-822.0968935
CAACGCA138150.022.008925
AACGCAG141850.021.4348436
GAGTACT68900.021.1270712-13
GTACTTT69850.020.32978414-15
TATACCG1908.243753E-620.0035045
AGAGTAC111100.019.17865210-11
ACGCAGA157800.019.0575187
GCGCGTT757.726304E-418.99975476-77
CGCAGAG158600.018.8116198
AGTACTT71700.018.57982812-13
ATTCCGT2603.277055E-718.272436
Produced by FastQC (version 0.11.5)