##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936985_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1992805 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25758867525924 33.0 31.0 34.0 31.0 34.0 2 32.38556456853531 34.0 31.0 34.0 31.0 34.0 3 32.496467541982284 34.0 31.0 34.0 31.0 34.0 4 36.122784216217845 37.0 35.0 37.0 35.0 37.0 5 36.033128680427836 37.0 35.0 37.0 35.0 37.0 6 36.06975243438269 37.0 35.0 37.0 35.0 37.0 7 36.02016052749767 37.0 35.0 37.0 35.0 37.0 8 35.981091978392264 37.0 35.0 37.0 35.0 37.0 9 37.683132067613236 39.0 37.0 39.0 35.0 39.0 10-11 37.60205263435208 39.0 37.0 39.0 35.0 39.0 12-13 37.51379562977813 39.0 37.0 39.0 35.0 39.0 14-15 38.74791336834261 40.0 38.0 41.0 35.0 41.0 16-17 38.6574592095062 40.0 38.0 41.0 34.0 41.0 18-19 38.80017513003028 40.0 38.0 41.0 35.0 41.0 20-21 38.8179643768457 40.0 38.0 41.0 35.0 41.0 22-23 38.73998032923442 40.0 38.0 41.0 34.5 41.0 24-25 38.641292299045816 40.0 38.0 41.0 34.0 41.0 26-27 38.467973785694035 40.0 38.0 41.0 34.0 41.0 28-29 38.40782188924656 40.0 38.0 41.0 34.0 41.0 30-31 38.4320876854484 40.0 38.0 41.0 34.0 41.0 32-33 38.29474609909148 40.0 38.0 41.0 34.0 41.0 34-35 38.16153687892192 40.0 38.0 41.0 34.0 41.0 36-37 38.02195021590171 40.0 37.5 41.0 33.0 41.0 38-39 37.87982542195549 40.0 37.0 41.0 33.0 41.0 40-41 37.72409744054235 40.0 37.0 41.0 33.0 41.0 42-43 37.50278928445081 40.0 37.0 41.0 33.0 41.0 44-45 37.262871429969316 40.0 37.0 41.0 32.0 41.0 46-47 36.95141320901945 40.0 36.5 41.0 31.0 41.0 48-49 36.62536374607651 39.0 36.0 41.0 30.5 41.0 50-51 36.34703445645711 39.0 36.0 40.5 30.0 41.0 52-53 36.12129786908403 39.0 35.0 40.0 30.0 41.0 54-55 35.85008994859005 39.0 35.0 40.0 29.0 41.0 56-57 35.555930459829234 38.0 35.0 40.0 28.0 41.0 58-59 35.209881297969446 38.0 34.0 40.0 28.0 41.0 60-61 34.87006556085518 38.0 34.0 40.0 27.0 41.0 62-63 34.5053090994854 37.0 34.0 40.0 26.0 41.0 64-65 34.12449587390638 37.0 33.5 39.0 26.0 40.5 66-67 33.7242710651569 36.0 33.0 39.0 26.0 40.0 68-69 33.40944798914093 36.0 33.0 39.0 25.5 40.0 70-71 32.93834293872205 35.0 32.0 38.0 24.5 40.0 72-73 32.42807951605902 35.0 31.5 37.5 23.0 39.5 74-75 31.9181984689922 35.0 31.0 37.0 22.0 39.0 76-77 30.495402460351116 33.5 29.5 35.5 20.5 37.5 78-79 31.253428458880826 34.0 31.0 36.0 21.5 38.0 80-81 31.025712249818724 34.0 31.0 36.0 20.0 37.0 82-83 30.708060999445507 34.0 31.0 35.0 19.5 37.0 84-85 30.355168217663042 34.0 30.0 35.0 18.0 36.5 86-87 30.011416320211964 34.0 30.0 35.0 17.0 36.0 88-89 29.62523929837591 34.0 30.0 35.0 10.0 36.0 90-91 29.17452485315924 34.0 29.0 35.0 6.0 35.0 92-93 28.690220066689918 34.0 29.0 35.0 2.0 35.0 94-95 28.1058814083666 33.0 28.5 35.0 2.0 35.0 96-97 27.450194825886125 33.0 27.0 34.5 2.0 35.0 98-99 26.633866835942303 33.0 25.5 34.0 2.0 35.0 100-101 23.109945529040722 28.5 12.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10037936944060988 1101 2 0.08321159734344263 1101 3 0.10095466335965142 1101 4 0.1424519371505113 1101 5 0.16916420221330242 1101 6 0.1560878910669743 1101 7 0.16535838299518701 1101 8 0.15049872138239806 1101 9 0.18287013999280077 1101 10-11 0.23975157343393505 1101 12-13 0.19803486383049318 1101 14-15 0.21860521844171643 1101 16-17 0.28316290992140836 1101 18-19 0.20629356212225503 1101 20-21 0.2554957395436048 1101 22-23 0.28878348859251446 1101 24-25 0.2755038950426325 1101 26-27 0.2281310732902 1101 28-29 0.2229750455059758 1101 30-31 0.25494365603619684 1101 32-33 0.37209473845298646 1101 34-35 0.3068244455898608 1101 36-37 0.3234317372282547 1101 38-39 0.1878802105278652 1101 40-41 0.22746017149409425 1101 42-43 0.21699431306900152 1101 44-45 0.3147970372716813 1101 46-47 0.29939618812756663 1101 48-49 0.2971793732937016 1101 50-51 0.2877314211276527 1101 52-53 0.23594888303301786 1101 54-55 0.28831864578148725 1101 56-57 0.354174081899302 1101 58-59 0.2824463453916195 1101 60-61 0.312457300050589 1101 62-63 0.31472024321689673 1101 64-65 0.25700293907699745 1101 66-67 0.03624085712688441 1101 68-69 0.11354890398072115 1101 70-71 0.20719176563775932 1101 72-73 0.17497523966391526 1101 74-75 0.1385767998426921 1101 76-77 0.013684054375215027 1101 78-79 0.13278322968199419 1101 80-81 0.4104055998951779 1101 82-83 0.32816286648087 1101 84-85 0.16907805875861115 1101 86-87 -0.2243207672510863 1101 88-89 -0.15769868668363785 1101 90-91 0.15432562480498646 1101 92-93 0.22716082336503973 1101 94-95 0.17683676796238856 1101 96-97 0.2068474009696324 1101 98-99 0.03571544176362451 1101 100-101 0.277219807433287 1102 1 0.22344871082795237 1102 2 0.18338721231666 1102 3 0.12749227118454343 1102 4 0.14493648010243732 1102 5 0.18015617877907886 1102 6 0.07325887538995346 1102 7 0.027644373195300886 1102 8 0.059048119485971995 1102 9 0.19339612110667304 1102 10-11 0.24282047813011332 1102 12-13 0.13086074797211467 1102 14-15 0.2291997677314157 1102 16-17 0.26636322801645207 1102 18-19 0.1802759615525673 1102 20-21 0.21065952650648967 1102 22-23 0.14379502739070205 1102 24-25 0.2163150029338965 1102 26-27 0.09941002874023042 1102 28-29 0.24490335881886693 1102 30-31 0.3031403800479424 1102 32-33 0.3042965181227544 1102 34-35 0.27732701009709615 1102 36-37 0.26560606855925784 1102 38-39 0.1890509450941309 1102 40-41 0.2806133403493547 1102 42-43 0.2889599653927277 1102 44-45 0.2035009282858269 1102 46-47 0.21788634371029758 1102 48-49 0.3159963577457745 1102 50-51 0.3893817307091325 1102 52-53 0.3319697070730925 1102 54-55 0.3331924947028142 1102 56-57 0.3387845485823533 1102 58-59 0.3993185984748422 1102 60-61 0.3546096819040727 1102 62-63 0.3694953941865933 1102 64-65 0.3823425957496269 1102 66-67 0.3112324728478413 1102 68-69 0.25473968420420334 1102 70-71 0.22118561097361322 1102 72-73 0.2644301820407904 1102 74-75 0.2707548871008605 1102 76-77 -0.023155251507994024 1102 78-79 0.1460562472812228 1102 80-81 0.3663022810613974 1102 82-83 0.38828882507067064 1102 84-85 0.21949597029838586 1102 86-87 0.1405286767625178 1102 88-89 0.03812761825206579 1102 90-91 0.3355195193260663 1102 92-93 0.18412126891261593 1102 94-95 0.1553963804257812 1102 96-97 0.11925778111887908 1102 98-99 0.32827640758660337 1102 100-101 0.520936386432922 1103 1 0.22724553474261455 1103 2 0.21919031589891347 1103 3 0.1846092923179441 1103 4 0.05051023826111134 1103 5 0.1321367098918813 1103 6 0.09751938349796774 1103 7 0.020981722595109886 1103 8 0.05933927508605308 1103 9 0.16747552821872347 1103 10-11 0.15052977687290792 1103 12-13 0.17618135749226127 1103 14-15 0.29924795130954607 1103 16-17 0.2617768358436905 1103 18-19 0.2672300701563657 1103 20-21 0.16042633465745126 1103 22-23 0.17083580411050292 1103 24-25 0.1605066146654508 1103 26-27 0.16835490039113665 1103 28-29 0.1547924523218569 1103 30-31 0.1521322238189029 1103 32-33 0.12720448953528773 1103 34-35 0.23957727594483913 1103 36-37 0.13472904482960502 1103 38-39 -0.0027761567902260254 1103 40-41 0.13385874801052466 1103 42-43 0.20511462046184903 1103 44-45 0.1743736593603913 1103 46-47 0.05839530730145981 1103 48-49 0.1178695817012212 1103 50-51 0.1841592585122811 1103 52-53 0.10947114210694764 1103 54-55 0.10715636153683761 1103 56-57 0.04634585398466129 1103 58-59 0.07342632825957907 1103 60-61 -0.10465914322315228 1103 62-63 -0.018894928383943466 1103 64-65 -0.02063971161517486 1103 66-67 0.004716524841335001 1103 68-69 -0.08135898195649105 1103 70-71 -0.1299418655776563 1103 72-73 0.10131408323818647 1103 74-75 0.057783877458430055 1103 76-77 -0.08102422484983052 1103 78-79 0.19629718361108317 1103 80-81 0.2959260634031331 1103 82-83 0.20705635733058259 1103 84-85 0.12155714542746665 1103 86-87 0.009217408930158655 1103 88-89 -0.07066730918676356 1103 90-91 0.06745934397257969 1103 92-93 0.13624166166105312 1103 94-95 -0.025229344121104447 1103 96-97 -0.05584170319089665 1103 98-99 -0.08243535261034296 1103 100-101 0.2863242549249989 1104 1 0.1184721282458554 1104 2 0.1268506239298901 1104 3 0.19523039910363593 1104 4 0.11081665058235046 1104 5 0.13636842645374259 1104 6 0.061410318193132696 1104 7 0.0401817269115341 1104 8 0.12907114407171605 1104 9 0.11189722149885739 1104 10-11 0.14053077504652123 1104 12-13 0.14478798806280935 1104 14-15 0.1343434657331457 1104 16-17 0.14156193176277299 1104 18-19 0.206638234137408 1104 20-21 0.14654173971360507 1104 22-23 0.08927611737514951 1104 24-25 0.0956655266022608 1104 26-27 0.029632778435896512 1104 28-29 0.09826318290400593 1104 30-31 0.21283547759509958 1104 32-33 0.19024643710689304 1104 34-35 0.1932547363753443 1104 36-37 0.18821064741083404 1104 38-39 0.1890746998855306 1104 40-41 0.2402983166916144 1104 42-43 0.19204771854993652 1104 44-45 0.2232353367776554 1104 46-47 0.3664573127671318 1104 48-49 0.19743157232917952 1104 50-51 0.26574349492295823 1104 52-53 0.19916378556742842 1104 54-55 0.1004235456351239 1104 56-57 0.3157685589272461 1104 58-59 0.30463948688555575 1104 60-61 0.3696683710277995 1104 62-63 0.2583529181239399 1104 64-65 0.2634294375362458 1104 66-67 0.28381288096809243 1104 68-69 0.1580791272329165 1104 70-71 0.07757033507142097 1104 72-73 0.13297588043628394 1104 74-75 0.09015626635766338 1104 76-77 0.0021336187714346977 1104 78-79 0.11864710011520785 1104 80-81 0.40946723266909757 1104 82-83 0.3486151807806479 1104 84-85 0.4443861852653832 1104 86-87 0.21304392096085323 1104 88-89 0.10568892548655029 1104 90-91 0.24492355135528854 1104 92-93 0.27675968678805063 1104 94-95 0.2298023951019914 1104 96-97 0.18581316074724796 1104 98-99 0.196508579708091 1104 100-101 0.4988934189141325 1105 1 0.12750336346962143 1105 2 0.166303984335606 1105 3 0.13984234618269653 1105 4 0.11692462488213096 1105 5 0.05153730434061288 1105 6 0.1103566277268726 1105 7 0.1710062292711001 1105 8 0.10151665163251522 1105 9 0.08341651984206777 1105 10-11 0.14449752793686343 1105 12-13 0.11838453540702432 1105 14-15 0.18452054877675295 1105 16-17 0.08943090885323812 1105 18-19 0.08780809478881935 1105 20-21 0.12010073803548948 1105 22-23 0.13839858281614426 1105 24-25 0.05364677990518629 1105 26-27 0.0026684082859844693 1105 28-29 0.15322938878390602 1105 30-31 0.11169463631157583 1105 32-33 0.08469567175583848 1105 34-35 0.06840840922601643 1105 36-37 0.07522332136479548 1105 38-39 0.0567524087461706 1105 40-41 0.1039990741620116 1105 42-43 0.14923024388394168 1105 44-45 0.10533557876023991 1105 46-47 0.07251522170380298 1105 48-49 0.03847821655914885 1105 50-51 0.12217179483302232 1105 52-53 0.014497230157125784 1105 54-55 0.11972837879693543 1105 56-57 0.1434217913881355 1105 58-59 0.11571134922434823 1105 60-61 0.015969096975567254 1105 62-63 0.06452764624119567 1105 64-65 -0.11159443622486265 1105 66-67 -0.02692057660150482 1105 68-69 -0.05943401492491063 1105 70-71 -0.2602855693964514 1105 72-73 -0.13045368965058657 1105 74-75 -0.04903378732353403 1105 76-77 -0.19624203833645737 1105 78-79 0.0944061930049962 1105 80-81 0.15678131865172418 1105 82-83 0.3129765229032344 1105 84-85 0.14347341952492698 1105 86-87 0.09530270325996426 1105 88-89 0.29910301239631565 1105 90-91 0.23553815004431655 1105 92-93 0.3127566828662651 1105 94-95 0.18507501954686845 1105 96-97 0.10168388085405766 1105 98-99 0.021445302651933673 1105 100-101 0.37818211990338924 1106 1 0.10185218566040533 1106 2 0.0908938268843471 1106 3 0.12762065358167263 1106 4 0.12936312048456244 1106 5 0.1261175368272376 1106 6 0.08312094287541072 1106 7 0.09765608473576748 1106 8 0.11710417239024906 1106 9 0.15376255895886004 1106 10-11 0.12039914396491014 1106 12-13 0.1585101965793001 1106 14-15 0.20713706116988817 1106 16-17 0.19157856553568564 1106 18-19 -0.007839315876047692 1106 20-21 0.21943149330948586 1106 22-23 0.10899582895674342 1106 24-25 0.08752628510231375 1106 26-27 0.1027766175854481 1106 28-29 0.13852541713742994 1106 30-31 0.0558586377876793 1106 32-33 0.1091790348162931 1106 34-35 0.04580727293227227 1106 36-37 0.10879568897657776 1106 38-39 0.20287820830333914 1106 40-41 0.2564894256596446 1106 42-43 0.1776969776929107 1106 44-45 -0.03706536071943134 1106 46-47 -0.08012024173183363 1106 48-49 0.016988964452622213 1106 50-51 -0.12660599746662626 1106 52-53 -0.14264669782122752 1106 54-55 -0.15749039234519557 1106 56-57 -0.08227416931055131 1106 58-59 0.03727037038186154 1106 60-61 -0.06818794681556284 1106 62-63 0.10359350976293769 1106 64-65 -0.07932711504890477 1106 66-67 -0.011187175100801028 1106 68-69 -0.06888608762310611 1106 70-71 -0.19460444663366872 1106 72-73 -0.3073820447154745 1106 74-75 -0.1576217232359518 1106 76-77 -0.206393339154463 1106 78-79 -0.13883847148851203 1106 80-81 -2.7688757251809193E-4 1106 82-83 -0.0028158843788865795 1106 84-85 0.10702168510091425 1106 86-87 -0.06113046452403026 1106 88-89 0.021423727077415577 1106 90-91 0.0712820072926128 1106 92-93 0.21743851292961125 1106 94-95 0.19567568686958126 1106 96-97 0.0722503747958747 1106 98-99 -0.14730085332321963 1106 100-101 -0.11809667184272499 1107 1 0.034175867814496996 1107 2 0.08702793274274256 1107 3 0.09904899426798863 1107 4 0.09780565643421113 1107 5 0.07123164656209724 1107 6 0.047085925431289866 1107 7 0.12084721931079656 1107 8 0.09281355098597999 1107 9 0.11477000270389937 1107 10-11 0.1369553812356301 1107 12-13 0.12040301757253502 1107 14-15 0.045337613874501415 1107 16-17 0.17996242373073557 1107 18-19 0.1583068347278882 1107 20-21 0.11526309607593532 1107 22-23 0.10819851833459637 1107 24-25 0.07273503377169277 1107 26-27 0.17653001334225138 1107 28-29 0.13053856252351181 1107 30-31 0.10166212845646072 1107 32-33 0.07153189817380934 1107 34-35 0.1405930172144778 1107 36-37 0.22161855507223294 1107 38-39 0.24720427556317048 1107 40-41 0.3225796789531117 1107 42-43 0.1645857222396998 1107 44-45 0.12274893407072085 1107 46-47 0.08781089247401752 1107 48-49 0.14273714620367883 1107 50-51 0.01643122048943013 1107 52-53 0.09995349431015654 1107 54-55 0.28943273664279445 1107 56-57 0.10177863759202666 1107 58-59 0.029120457910089215 1107 60-61 0.15331182782616537 1107 62-63 0.11982247760669651 1107 64-65 0.014438556195685237 1107 66-67 0.10116170795287616 1107 68-69 0.13315432765141821 1107 70-71 0.2465521450571444 1107 72-73 0.22943220854106272 1107 74-75 0.1018396705087774 1107 76-77 0.10946972916370257 1107 78-79 0.41718343979570705 1107 80-81 0.4222578429820629 1107 82-83 0.41149163504961095 1107 84-85 0.4663140986033376 1107 86-87 0.32928609830862143 1107 88-89 0.2507949852072322 1107 90-91 0.3977950803794492 1107 92-93 0.10087223837094683 1107 94-95 0.18193402435627704 1107 96-97 0.3496482290467604 1107 98-99 0.2478204478784889 1107 100-101 0.40677186275938837 1108 1 0.08864796030829325 1108 2 0.06448537618012296 1108 3 0.13642347048308068 1108 4 0.13464481062541012 1108 5 0.1947795086582218 1108 6 0.10600826681906739 1108 7 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-0.6931107804647567 2316 82-83 -0.4706733196884194 2316 84-85 -0.6190735490949599 2316 86-87 -0.47553740032488534 2316 88-89 -0.613648333191172 2316 90-91 -0.700517247538702 2316 92-93 -0.9430521929334716 2316 94-95 -0.8257683845355928 2316 96-97 -0.3020812252696352 2316 98-99 -0.32364584185908285 2316 100-101 -0.6605416030579185 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 13.0 5 33.0 6 60.0 7 164.0 8 415.0 9 800.0 10 1447.0 11 2404.0 12 3656.0 13 4972.0 14 6566.0 15 8794.0 16 11769.0 17 16426.0 18 15148.0 19 9818.0 20 9005.0 21 9343.0 22 10161.0 23 11172.0 24 12900.0 25 15311.0 26 19288.0 27 23801.0 28 29855.0 29 37447.0 30 47200.0 31 59440.0 32 76388.0 33 100805.0 34 138891.0 35 199580.0 36 311187.0 37 460167.0 38 317986.0 39 20383.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.79488673753195 19.552250717913736 15.103118062112275 22.549744482442048 2 15.57994924431031 20.840093482003912 39.70350527404509 23.876451999640686 3 19.555138721829223 24.753148959680367 29.881107044807788 25.81060527368262 4 13.341596393023902 17.26621520921515 37.08481261337662 32.30737578438433 5 13.872513302863704 37.603146119738824 34.12347626761086 14.400864309786613 6 31.742945245520758 36.533228288768846 17.455044522670306 14.268781943040088 7 28.221677484751396 32.77962469985774 20.868624877998602 18.13007293739227 8 26.244815724569136 35.03880209052065 19.697963423415736 19.018418761494477 9 26.23900795060628 16.989729045104284 20.39076597598158 36.38049702830785 10-11 24.76351218253846 26.869462639353852 27.386038304288814 20.98098687381888 12-13 25.844174417466835 24.153968903129005 27.864141248140186 22.13771543126397 14-15 22.860791698134037 25.569511316962775 26.258339375904814 25.31135760899837 16-17 22.086732018436326 28.780588165926922 26.829443924518454 22.303235891118298 18-19 22.033902557683298 27.537015138753297 29.01970318639682 21.40937911716658 20-21 23.04028707555329 26.474299416570425 29.003899315806947 21.481514192069344 22-23 22.624337886218925 26.401701554949643 28.960808484490524 22.013152074340912 24-25 22.55359810336776 26.560630176537053 29.022934786454474 21.862836933640708 26-27 22.375070037359112 26.676900756842585 29.035274740908818 21.912754464889485 28-29 22.202233591487754 27.033828541205473 28.911212593138448 21.852725274168325 30-31 22.462050472576653 26.64735662431555 28.86602930479582 22.02456359831198 32-33 22.344277859965995 26.68461035089797 28.890443997037206 22.080667792098822 34-35 22.631943416440645 26.73726230112831 28.775143579025542 21.8556507034055 36-37 22.68555680654527 26.611520519590925 28.809102677371705 21.8938199964921 38-39 22.452490665759278 26.8419112489928 28.79623614104584 21.90936194420208 40-41 22.63291851939576 26.821913697491095 28.60580264464585 21.9393651384673 42-43 22.44296356141218 26.858021733185133 28.373448480910074 22.32556622449261 44-45 22.62629810744152 26.80917099264604 28.36930858764405 22.19522231226839 46-47 22.880575792998744 27.084184836457453 27.96656161344882 22.06867775709498 48-49 22.845731407906367 27.102392665760206 27.816260612610154 22.235615313723272 50-51 22.66384230549772 27.35680845180545 27.829818689863767 22.14953055283307 52-53 22.942335050343612 27.39324720682656 27.450804268355412 22.21361347447442 54-55 22.79854862898331 27.207535968754527 27.50980874792026 22.484106654341907 56-57 22.887522641728626 27.478922711632887 27.41867858869674 22.214876057941748 58-59 23.051720854564937 27.278103435687463 27.380170699218464 22.29000501052914 60-61 22.88102209743748 27.25037824329512 27.824003332053703 22.044596327213696 62-63 22.620742503478965 27.288249909293512 27.900938981507924 22.190068605719603 64-65 22.678987658100013 27.3845408858368 27.672125471383303 22.26434598467989 66-67 22.738559060918647 27.308059792568685 27.4408658159855 22.51251533052717 68-69 22.733532768114788 27.291519940425484 27.65093911903286 22.324008172426865 70-71 22.910773526269544 27.16602861425454 27.44370561169982 22.4794922477761 72-73 22.846565519456245 27.10616442652442 27.523440577477476 22.52382947654186 74-75 22.741688223383623 27.69425508265987 27.118734647895803 22.445322046060703 76-77 22.862121482031608 27.395404969377335 27.173255787696238 22.56921776089482 78-79 22.821342617590442 27.352937929428673 27.189571871029383 22.6361475819515 80-81 22.954455356112348 27.527863225560402 27.036637444361368 22.481043973965885 82-83 22.927173964593848 27.470868557012114 27.19466656897007 22.407290909423967 84-85 22.82685200263872 27.468172145053266 27.11000291824864 22.594972934059374 86-87 22.72885348439015 27.48309342625843 27.11530393693833 22.67274915241309 88-89 22.799750602480678 27.389593298884364 26.99971522516657 22.810940873468393 90-91 22.839739420872824 27.266543623218077 27.118836285974652 22.774880669934443 92-93 22.849384936386052 27.282063145259787 27.160400280609913 22.70815163774424 94-95 22.723654026273024 27.363773014362426 27.126971060131588 22.785601899232965 96-97 22.54417044471158 27.333850705314504 27.265252499857613 22.856726350116308 98-99 22.51883689245847 27.480862018351388 26.925804096318434 23.074496992871712 100-101 22.575911995226818 27.44990593641814 26.84502868073631 23.129153387618732 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 192.0 1 147.5 2 119.0 3 130.5 4 129.0 5 148.5 6 225.5 7 463.5 8 862.5 9 1156.5 10 1198.0 11 1199.0 12 1344.0 13 1542.5 14 1729.0 15 1857.0 16 2047.0 17 2267.5 18 2387.5 19 2684.5 20 3069.5 21 3474.0 22 4175.0 23 5251.0 24 6569.5 25 8371.0 26 10828.5 27 13816.0 28 16747.0 29 19660.5 30 22850.5 31 26665.0 32 30608.5 33 34153.0 34 38112.5 35 42182.0 36 46725.0 37 50877.5 38 53987.0 39 56731.5 40 59300.0 41 61941.0 42 64320.5 43 66997.5 44 70254.5 45 72740.0 46 74885.0 47 78767.0 48 83737.0 49 82939.0 50 77242.5 51 73402.0 52 71334.0 53 70057.5 54 68040.5 55 63381.5 56 56696.5 57 50222.0 58 45679.0 59 40822.0 60 34020.0 61 27690.0 62 22255.5 63 16988.5 64 12507.5 65 9117.5 66 6383.5 67 4756.5 68 3490.0 69 2256.0 70 1467.5 71 913.0 72 571.0 73 392.0 74 264.5 75 169.5 76 104.5 77 47.5 78 22.5 79 11.0 80 6.5 81 6.0 82 3.5 83 2.5 84 2.5 85 1.5 86 0.5 87 1.5 88 1.5 89 0.0 90 0.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02880362102664335 2 0.006122023981272628 3 2.0072209774664354E-4 4 0.0 5 5.0180524436660884E-5 6 0.0 7 0.0 8 0.0 9 5.0180524436660884E-5 10-11 0.01307202661575016 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.7599288440163485E-4 20-21 9.785202265148872E-4 22-23 0.001982130715248105 24-25 0.011064805638283725 26-27 0.005946392145744315 28-29 2.7599288440163485E-4 30-31 0.002985741203981323 32-33 0.004466066674862819 34-35 0.0 36-37 0.008053974172084073 38-39 0.013824734482300075 40-41 0.0012043325864798614 42-43 0.0 44-45 0.0 46-47 2.7599288440163485E-4 48-49 0.0034875464483479315 50-51 0.007125634470005846 52-53 0.0 54-55 0.0034373659239112704 56-57 0.003989351692714541 58-59 7.527078665499134E-5 60-61 0.0020072209774664356 62-63 0.005720579785779341 64-65 0.0 66-67 0.0024588456973963836 68-69 3.2617340883829576E-4 70-71 0.0011039715376065394 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0020574015019030963 80-81 0.0017563183552831312 82-83 0.012118596651453605 84-85 0.008605959940887342 86-87 0.004466066674862819 88-89 1.2545131109165222E-4 90-91 6.523468176765915E-4 92-93 1.5054157330998268E-4 94-95 2.5090262218330442E-5 96-97 0.002082491764121427 98-99 0.0020072209774664356 100-101 2.0072209774664354E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1992805.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.89413867628553 #Duplication Level Percentage of deduplicated Percentage of total 1 77.69770612638487 48.090325978157445 2 13.217221847996377 16.361371239502372 3 4.084860173373715 7.5848670613208515 4 1.7738659093071973 4.3916761033518 5 0.9213812649306938 2.8514049892675866 6 0.5531522712141274 2.05421300301777 7 0.3688872950603629 1.598237297747017 8 0.2480027422536296 1.2279932896916197 9 0.18604937450378514 1.036382920755611 >10 0.8877563559570938 9.57134621504167 >50 0.041601643509535734 1.759663298661339 >100 0.017977293177778443 1.9661525919760356 >500 8.902487177978488E-4 0.38245226449424136 >1k 5.665219113258854E-4 0.8205066263805532 >5k 8.093170161798363E-5 0.30340712063412756 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6057 0.303943436512855 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4939 0.24784161019266812 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3329 0.1670509658496441 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2987 0.14988922649230607 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.018052443666089E-5 0.0 0.0 0.0 0.0 7 1.0036104887332178E-4 0.0 0.0 0.0 0.0 8 1.0036104887332178E-4 0.0 0.0 0.0 0.0 9 1.0036104887332178E-4 0.0 0.0 5.018052443666089E-5 0.0 10-11 1.0036104887332178E-4 0.0 0.0 5.018052443666089E-5 0.0 12-13 1.0036104887332178E-4 0.0 0.0 5.018052443666089E-5 0.0 14-15 2.25812359964974E-4 0.0 0.0 1.0036104887332178E-4 0.0 16-17 2.5090262218330445E-4 0.0 0.0 1.756318355283131E-4 0.0 18-19 2.759928844016349E-4 0.0 0.0 2.0072209774664356E-4 0.0 20-21 3.010831466199653E-4 0.0 0.0 2.5090262218330445E-4 0.0 22-23 3.010831466199653E-4 0.0 0.0 3.010831466199653E-4 0.0 24-25 3.010831466199653E-4 0.0 0.0 3.7635393327495667E-4 0.0 26-27 3.512636710566262E-4 0.0 0.0 4.2653445771161753E-4 2.5090262218330445E-5 28-29 4.0144419549328707E-4 0.0 0.0 8.028883909865741E-4 5.018052443666089E-5 30-31 5.018052443666089E-4 0.0 0.0 0.001354874159789844 5.018052443666089E-5 32-33 5.018052443666089E-4 0.0 0.0 0.002709748319579688 5.018052443666089E-5 34-35 5.268955065849394E-4 0.0 0.0 0.0038639003816228886 5.018052443666089E-5 36-37 5.519857688032698E-4 0.0 0.0 0.005118413492539411 5.018052443666089E-5 38-39 6.021662932399306E-4 0.0 0.0 0.006924912372259203 5.018052443666089E-5 40-41 6.021662932399306E-4 0.0 0.0 0.010763722491663762 5.018052443666089E-5 42-43 6.021662932399306E-4 0.0 0.0 0.015129428117653259 5.018052443666089E-5 44-45 6.021662932399306E-4 0.0 0.0 0.024187012778470546 5.018052443666089E-5 46-47 6.021662932399306E-4 0.0 0.0 0.04220182105123181 5.018052443666089E-5 48-49 6.021662932399306E-4 0.0 0.0 0.054847313209270346 5.018052443666089E-5 50-51 6.021662932399306E-4 0.0 0.0 0.07032800499798023 5.018052443666089E-5 52-53 6.021662932399306E-4 0.0 0.0 0.08889479903954475 5.018052443666089E-5 54-55 6.021662932399306E-4 0.0 0.0 0.11162657660935214 5.018052443666089E-5 56-57 6.021662932399306E-4 0.0 0.0 0.13511106204570944 5.018052443666089E-5 58-59 6.021662932399306E-4 0.0 0.0 0.16577136247650925 5.018052443666089E-5 60-61 6.021662932399306E-4 0.0 0.0 0.2025787771508 5.018052443666089E-5 62-63 6.021662932399306E-4 0.0 0.0 0.24902085251692965 5.018052443666089E-5 64-65 6.021662932399306E-4 0.0 0.0 0.28379595595153567 5.018052443666089E-5 66-67 6.523468176765915E-4 0.0 0.0 0.34190500324918893 5.018052443666089E-5 68-69 6.523468176765915E-4 0.0 0.0 0.41810412960625853 5.018052443666089E-5 70-71 6.523468176765915E-4 0.0 0.0 0.4687613690250677 5.018052443666089E-5 72-73 6.77437079894922E-4 0.0 0.0 0.5582583343578524 5.018052443666089E-5 74-75 7.025273421132524E-4 0.0 0.0 0.6302172064000241 5.018052443666089E-5 76-77 7.025273421132524E-4 0.0 0.0 0.6882258926488041 5.018052443666089E-5 78-79 7.025273421132524E-4 0.0 0.0 0.7660809763122834 5.018052443666089E-5 80-81 7.025273421132524E-4 0.0 0.0 0.8486781195350273 5.018052443666089E-5 82-83 7.025273421132524E-4 0.0 0.0 0.9501180496837373 5.018052443666089E-5 84-85 7.276176043315829E-4 0.0 0.0 1.1029930173800246 5.018052443666089E-5 86-87 7.527078665499133E-4 0.0 0.0 1.3258949069276724 5.018052443666089E-5 88-89 7.527078665499133E-4 0.0 0.0 1.5976224467521911 5.018052443666089E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11875 0.0 25.763218 1 GTACATG 8000 0.0 24.049881 1 TACATGG 8090 0.0 23.073614 2 GGTATCA 8930 0.0 22.609207 1 ACATGGG 8045 0.0 22.43347 3 ATCAACG 13710 0.0 22.170803 3 TATCAAC 13775 0.0 22.102327 2 TCAACGC 13775 0.0 22.10011 4 TATTCCG 215 4.4174158E-8 22.096893 5 CAACGCA 13815 0.0 22.00892 5 AACGCAG 14185 0.0 21.434843 6 GAGTACT 6890 0.0 21.12707 12-13 GTACTTT 6985 0.0 20.329784 14-15 TATACCG 190 8.243753E-6 20.003504 5 AGAGTAC 11110 0.0 19.178652 10-11 ACGCAGA 15780 0.0 19.057518 7 GCGCGTT 75 7.726304E-4 18.999754 76-77 CGCAGAG 15860 0.0 18.811619 8 AGTACTT 7170 0.0 18.579828 12-13 ATTCCGT 260 3.277055E-7 18.27243 6 >>END_MODULE