FastQCFastQC Report
Thu 26 May 2016
SRR936984_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936984_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018585
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58520.2899060480485092No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44130.2186184877030197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28440.14089077249657556No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27830.13786885367720458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103550.029.9284171
GGTATCA72350.027.1986961
TATCAAC122000.025.2490182
TCAACGC122100.025.1906574
ATCAACG122000.025.171093
CAACGCA122500.025.1084025
AACGCAG125150.024.6122866
GTACATG85900.024.125681
TACATGG84650.023.6420142
ACATGGG85850.022.702463
TAGGTCG1050.002186314222.6376635
GAGTACT61100.022.47878612-13
ACGCAGA138400.022.1490967
CGCAGAG139750.021.833118
AGAGTAC104750.020.8237410-11
GCAGAGT147550.020.388549
GTACTTT66500.020.36909714-15
CATGGGG42550.020.3339964
AGTACTT66400.019.2530912-13
CGTATGA1759.882694E-519.0146942
Produced by FastQC (version 0.11.5)