##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936984_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2018585 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.648701441851593 33.0 31.0 34.0 30.0 34.0 2 31.740586103632 33.0 31.0 34.0 30.0 34.0 3 31.76829511761952 33.0 31.0 34.0 30.0 34.0 4 35.36406938523768 37.0 35.0 37.0 33.0 37.0 5 35.360816611636366 37.0 35.0 37.0 33.0 37.0 6 35.4182647745822 37.0 35.0 37.0 33.0 37.0 7 35.35647941503578 37.0 35.0 37.0 33.0 37.0 8 35.31368854915696 37.0 35.0 37.0 33.0 37.0 9 36.9218824077262 39.0 37.0 39.0 33.0 39.0 10-11 36.786053844648606 39.0 37.0 39.0 32.5 39.0 12-13 36.69965347012882 39.0 37.0 39.0 32.0 39.0 14-15 37.839268844264666 40.0 38.0 41.0 32.5 41.0 16-17 37.76485260714808 40.0 37.0 41.0 32.0 41.0 18-19 37.710567798730295 40.0 37.0 41.0 32.0 41.0 20-21 37.6288345548986 40.0 37.0 41.0 32.0 41.0 22-23 37.51146025557507 40.0 37.0 41.0 32.0 41.0 24-25 37.47410983436417 40.0 37.0 41.0 32.0 41.0 26-27 37.50510208883946 40.0 37.0 41.0 32.0 41.0 28-29 37.42690622391427 40.0 37.0 41.0 32.0 41.0 30-31 37.276488976188766 40.0 37.0 41.0 31.0 41.0 32-33 37.10104801135449 40.0 36.0 41.0 31.0 41.0 34-35 36.932284991714496 39.0 36.0 41.0 30.0 41.0 36-37 36.762858883822084 39.0 36.0 41.0 30.0 41.0 38-39 36.60866720995153 39.0 36.0 41.0 30.0 41.0 40-41 36.42649554019276 39.0 35.0 41.0 30.0 41.0 42-43 36.213790600841676 39.0 35.0 40.5 30.0 41.0 44-45 35.97649838872279 39.0 35.0 40.0 28.5 41.0 46-47 35.66776430024002 39.0 35.0 40.0 27.5 41.0 48-49 35.45710287156597 38.5 35.0 40.0 27.0 41.0 50-51 34.78077093607651 38.0 34.0 39.5 26.0 40.5 52-53 34.966932034073366 38.0 34.5 40.0 26.5 40.5 54-55 35.357794692816995 39.0 35.0 40.0 27.0 41.0 56-57 35.23778711325012 39.0 35.0 40.5 26.0 41.0 58-59 35.01669312909786 38.0 34.0 40.0 26.0 41.0 60-61 34.72864159795104 38.0 34.0 40.0 26.0 41.0 62-63 34.41796332579505 38.0 34.0 40.0 25.5 41.0 64-65 34.08358577914727 37.0 34.0 40.0 24.5 41.0 66-67 33.70593435500611 36.5 33.0 39.5 23.0 41.0 68-69 33.30910984674908 36.0 33.0 39.0 22.5 41.0 70-71 32.892063995323454 35.5 32.5 39.0 22.0 40.5 72-73 32.497632252295546 35.0 32.0 38.0 21.5 40.0 74-75 32.09835082495907 35.0 32.0 37.0 20.0 39.5 76-77 31.64384135421595 35.0 31.0 37.0 19.0 39.0 78-79 31.17428892020896 35.0 31.0 36.0 17.5 39.0 80-81 30.73993985886153 35.0 31.0 36.0 14.0 37.5 82-83 30.331126506934314 34.0 30.0 35.5 9.5 37.0 84-85 29.949774718428998 34.0 30.0 35.0 7.0 37.0 86-87 29.606764144190116 34.0 30.0 35.0 4.5 36.0 88-89 29.23626797979773 34.0 29.0 35.0 2.0 36.0 90-91 28.868158883574385 34.0 29.0 35.0 2.0 35.5 92-93 28.47025614477468 34.0 29.0 35.0 2.0 35.0 94-95 28.091042735381468 34.0 29.0 35.0 2.0 35.0 96-97 27.57870017859045 34.0 27.0 35.0 2.0 35.0 98-99 26.99676010670841 34.0 26.5 35.0 2.0 35.0 100-101 24.804345122945033 31.0 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25564905229329327 1101 2 0.18123766760397686 1101 3 0.28066556303987156 1101 4 0.3018409379619058 1101 5 0.22325423648199916 1101 6 0.1831251554123483 1101 7 0.20221065439467623 1101 8 0.26864167460664845 1101 9 0.1401788397233048 1101 10-11 0.20383412264056489 1101 12-13 0.34661830053314446 1101 14-15 0.43759571511119333 1101 16-17 0.5258271853982848 1101 18-19 0.5444057549495369 1101 20-21 0.49299458031417487 1101 22-23 0.5759741603577027 1101 24-25 0.5995915148037767 1101 26-27 0.40088467545452033 1101 28-29 0.3795082157646661 1101 30-31 0.2646128238666776 1101 32-33 0.26230646160799864 1101 34-35 0.3463963069575229 1101 36-37 0.3467414477752584 1101 38-39 0.22973887017828076 1101 40-41 0.284625661154692 1101 42-43 0.30307862952972897 1101 44-45 0.4732622183940336 1101 46-47 0.46133650132511406 1101 48-49 0.39402222782081253 1101 50-51 0.5537484597317714 1101 52-53 0.4214922116979025 1101 54-55 0.5292576459591771 1101 56-57 0.5011796284043797 1101 58-59 0.5732594084897045 1101 60-61 0.5040415409783492 1101 62-63 0.4707479416568887 1101 64-65 0.3634328664471056 1101 66-67 0.5161066813096369 1101 68-69 0.4702375831555372 1101 70-71 0.5136433286496214 1101 72-73 0.30405304900904895 1101 74-75 0.33396562908941974 1101 76-77 0.5002321758787787 1101 78-79 0.38591513096428187 1101 80-81 0.45277294359595643 1101 82-83 0.25924944902693525 1101 84-85 0.2399949264490111 1101 86-87 0.35903879625184487 1101 88-89 0.5046699598628841 1101 90-91 0.5374060739195876 1101 92-93 0.5683812131095998 1101 94-95 0.46109072465546674 1101 96-97 0.6870427300847481 1101 98-99 0.6265461881840189 1101 100-101 1.1200825315673661 1102 1 0.2580206446948452 1102 2 0.30538582597832686 1102 3 0.2633513241705465 1102 4 0.22459467942215383 1102 5 0.2438474370388164 1102 6 0.09002962334324138 1102 7 0.0969671361472706 1102 8 0.021623653770184603 1102 9 0.1911052656198109 1102 10-11 0.1319672279625692 1102 12-13 0.18519099565743602 1102 14-15 0.07410515639066517 1102 16-17 0.26431639883715263 1102 18-19 0.04382283048904867 1102 20-21 0.07678067051002557 1102 22-23 0.1066529839172361 1102 24-25 -0.04269874094075732 1102 26-27 0.23863962488492518 1102 28-29 0.09846124892019503 1102 30-31 0.060391831015415676 1102 32-33 0.07781703960409203 1102 34-35 0.1256754809211813 1102 36-37 0.15541613937175214 1102 38-39 0.1376325376313332 1102 40-41 -0.03638581994289325 1102 42-43 0.058261860603103344 1102 44-45 0.25389344681618553 1102 46-47 0.3017959307846638 1102 48-49 0.387494828422831 1102 50-51 0.34527535627274375 1102 52-53 0.4662910187948981 1102 54-55 0.2913289046937706 1102 56-57 0.4509006077100608 1102 58-59 0.0880708668010044 1102 60-61 0.2204310239559888 1102 62-63 0.16193742300163194 1102 64-65 0.40086815839921286 1102 66-67 0.028523352707978233 1102 68-69 0.14750432708775918 1102 70-71 0.10877376159280772 1102 72-73 0.12029759412386198 1102 74-75 0.3188650743880359 1102 76-77 0.23354558048791674 1102 78-79 0.21556610366786444 1102 80-81 0.3392546442397979 1102 82-83 0.3063390388477707 1102 84-85 0.27133377302492434 1102 86-87 0.23217057113668105 1102 88-89 0.45710859368018575 1102 90-91 0.2529311438463999 1102 92-93 0.27405770794019446 1102 94-95 0.4837968524227101 1102 96-97 0.19877794673818272 1102 98-99 0.2161178245252522 1102 100-101 0.035885249073430714 1103 1 0.2527960690558224 1103 2 0.2894120879081612 1103 3 0.2116648523017055 1103 4 0.188847182713765 1103 5 0.22056389191728698 1103 6 0.28363585517418954 1103 7 0.2226357789887956 1103 8 0.26043343692204957 1103 9 0.08107902686524682 1103 10-11 0.1159755652670853 1103 12-13 0.07427160360278151 1103 14-15 0.35395662823778196 1103 16-17 0.3685704555962914 1103 18-19 0.4633867985882887 1103 20-21 0.19558988514346964 1103 22-23 0.22928403646572093 1103 24-25 0.3304074952355549 1103 26-27 0.39380577348767076 1103 28-29 0.3349575785363115 1103 30-31 0.27454900789054193 1103 32-33 0.23390666852451858 1103 34-35 0.4519878531855994 1103 36-37 0.37161047707314765 1103 38-39 0.31397907531795255 1103 40-41 0.30367247673246567 1103 42-43 0.36508283234230987 1103 44-45 0.2277990850704512 1103 46-47 0.22662901450099326 1103 48-49 0.3464747760177431 1103 50-51 0.33744636878594747 1103 52-53 0.3965834183192314 1103 54-55 0.284481607292733 1103 56-57 0.3379767355854213 1103 58-59 0.3438349917678707 1103 60-61 0.45167009717681594 1103 62-63 0.3358737322814491 1103 64-65 0.3875899037579913 1103 66-67 0.43820759323254777 1103 68-69 0.3807155617359541 1103 70-71 0.34750523766940233 1103 72-73 0.27636739732233906 1103 74-75 0.35674650679373343 1103 76-77 0.24842953754993147 1103 78-79 0.24961048938552466 1103 80-81 0.15629464403701476 1103 82-83 0.11062349504723201 1103 84-85 4.574566071724462E-5 1103 86-87 0.07831047591527707 1103 88-89 -0.022863262847490518 1103 90-91 0.12594205360622723 1103 92-93 0.12484262547243219 1103 94-95 0.19290761357110142 1103 96-97 0.191648019638901 1103 98-99 0.17787373530624606 1103 100-101 0.3340937099311212 1104 1 0.1444093645961857 1104 2 0.17121874548620752 1104 3 0.16460129173884752 1104 4 0.22678715298192742 1104 5 0.294169458860857 1104 6 0.22846662048181798 1104 7 0.15715637760476397 1104 8 0.1861502516641096 1104 9 0.23642981212486802 1104 10-11 0.303982509234757 1104 12-13 0.1832331189457932 1104 14-15 0.031217568559732456 1104 16-17 0.4030690040324316 1104 18-19 0.4045370753802189 1104 20-21 0.29479616623964944 1104 22-23 0.22146419151403762 1104 24-25 0.2198689279122732 1104 26-27 0.11506251464273021 1104 28-29 0.15062149036081962 1104 30-31 0.4025089686871084 1104 32-33 0.19069794877302115 1104 34-35 0.19605952485193967 1104 36-37 0.09258673083340341 1104 38-39 0.16101992016735522 1104 40-41 0.14738933140655064 1104 42-43 0.18353848258266936 1104 44-45 0.22476941838369413 1104 46-47 0.18002265802437023 1104 48-49 0.30384717220501045 1104 50-51 0.16515713978213142 1104 52-53 0.31910689764495004 1104 54-55 0.5720813672802905 1104 56-57 0.5935968167665138 1104 58-59 0.31433889179491104 1104 60-61 0.40314341348183547 1104 62-63 0.345935287136129 1104 64-65 0.3212758706662626 1104 66-67 0.11606453642960446 1104 68-69 0.2941274478862681 1104 70-71 0.25642880008174274 1104 72-73 0.22412747385170206 1104 74-75 0.21038725594640795 1104 76-77 0.3784191131916792 1104 78-79 0.33316779813567976 1104 80-81 0.16253463476157748 1104 82-83 0.21540055529413493 1104 84-85 0.3919906696007338 1104 86-87 0.4240199908043145 1104 88-89 0.4332735176230891 1104 90-91 0.47251606164587656 1104 92-93 0.34682155818067884 1104 94-95 0.37755142347788606 1104 96-97 0.40850698465705193 1104 98-99 0.5478447530682864 1104 100-101 0.5414199566157123 1105 1 0.13711240591349494 1105 2 0.1932748356931384 1105 3 0.11591621942815067 1105 4 0.16968992641627523 1105 5 0.166114717902893 1105 6 0.10585017891943949 1105 7 0.11954852127814064 1105 8 0.04204810210989507 1105 9 0.18789028562078158 1105 10-11 0.20597609341825773 1105 12-13 0.11767456724457759 1105 14-15 0.1977868768827875 1105 16-17 0.2611913451170551 1105 18-19 0.2127047520167693 1105 20-21 0.16669225440107738 1105 22-23 0.13780878891270731 1105 24-25 0.1211524845797527 1105 26-27 0.19003728998097813 1105 28-29 0.16709570220076841 1105 30-31 0.23844835869283543 1105 32-33 0.0771997553111774 1105 34-35 -0.043030931866873345 1105 36-37 -0.023349354889134588 1105 38-39 0.19574931314299704 1105 40-41 0.18409539378276207 1105 42-43 0.31258844216420556 1105 44-45 0.18747822252821322 1105 46-47 0.18060090758035585 1105 48-49 0.2772172066836305 1105 50-51 0.004926941387459749 1105 52-53 0.24819363704518338 1105 54-55 0.03924308637294871 1105 56-57 0.12035757683031534 1105 58-59 0.0438390599593248 1105 60-61 0.19944418979169853 1105 62-63 0.05075462348658988 1105 64-65 0.209887107982631 1105 66-67 -0.008725258055775953 1105 68-69 0.002772278748267354 1105 70-71 -0.10115659498661245 1105 72-73 0.10576234662696749 1105 74-75 0.03642068034420731 1105 76-77 0.1026737318124944 1105 78-79 0.1086043863846271 1105 80-81 -0.10210501152277729 1105 82-83 0.09551801469374155 1105 84-85 0.10261118644569578 1105 86-87 0.28613992448263303 1105 88-89 0.26131311394982504 1105 90-91 0.373601951373292 1105 92-93 0.29921922866103756 1105 94-95 0.39572399753415155 1105 96-97 0.06283667737838528 1105 98-99 0.39771327013353286 1105 100-101 0.4799750407864245 1106 1 0.15220494071726165 1106 2 0.22583198765010692 1106 3 0.2674742657416651 1106 4 0.19435014599474698 1106 5 0.1586531677277776 1106 6 0.2568784744731829 1106 7 0.2507980253070343 1106 8 0.26776696531194943 1106 9 0.24863789906144262 1106 10-11 0.29937737211219684 1106 12-13 0.2875197384698822 1106 14-15 0.19349417993668538 1106 16-17 0.37228458339551906 1106 18-19 0.3856544342735546 1106 20-21 0.4059594396658568 1106 22-23 0.1545291813143237 1106 24-25 0.14560359201620088 1106 26-27 -0.03740594411523546 1106 28-29 -0.08821092054161994 1106 30-31 0.16289131611760155 1106 32-33 0.21734564052039929 1106 34-35 0.3628707489658112 1106 36-37 0.0502742046499165 1106 38-39 0.05956690730243963 1106 40-41 0.16532463001942688 1106 42-43 0.08492644459634136 1106 44-45 0.08718160499753225 1106 46-47 0.12227792261712978 1106 48-49 0.042758528315260946 1106 50-51 0.10513562458150005 1106 52-53 0.009867992604462472 1106 54-55 0.16242836830973317 1106 56-57 0.19371197150964292 1106 58-59 0.32221236052102853 1106 60-61 0.4754582764434758 1106 62-63 0.3757492080182985 1106 64-65 0.02242826017981514 1106 66-67 0.05497673462207331 1106 68-69 0.13062665948679353 1106 70-71 0.11232116513280488 1106 72-73 0.12009242638983864 1106 74-75 0.40169991475784883 1106 76-77 0.2778839699429696 1106 78-79 0.3335404195165701 1106 80-81 0.3467611717195105 1106 82-83 0.1331796476558189 1106 84-85 0.09947235957553602 1106 86-87 0.2401513387884755 1106 88-89 0.2895711347409353 1106 90-91 0.3414512276308095 1106 92-93 0.5212641523833597 1106 94-95 0.5659262679558275 1106 96-97 0.3967094128011226 1106 98-99 0.5301831311758356 1106 100-101 1.0009473800757007 1107 1 0.31858101360531066 1107 2 0.3690066275496342 1107 3 0.3271997612290214 1107 4 0.2067026959852214 1107 5 0.12310443315014652 1107 6 0.22602399755400882 1107 7 0.2918844793799167 1107 8 0.3225559019442841 1107 9 0.3545270208967324 1107 10-11 0.41392580949909785 1107 12-13 0.3780642841671664 1107 14-15 0.3822883619088131 1107 16-17 0.39948999992067513 1107 18-19 0.47767979454896903 1107 20-21 0.5931996574657603 1107 22-23 0.6895723198933297 1107 24-25 0.661596127039985 1107 26-27 0.7958519070011718 1107 28-29 0.5869821386225098 1107 30-31 0.661284211881501 1107 32-33 0.5206293247057232 1107 34-35 0.5682656244704489 1107 36-37 0.5281453043496427 1107 38-39 0.6726778294225113 1107 40-41 0.7365107752894886 1107 42-43 0.8262175425859581 1107 44-45 0.7452964754462172 1107 46-47 0.6189641038606126 1107 48-49 0.6892815389469931 1107 50-51 0.7100571817559356 1107 52-53 0.708814550976193 1107 54-55 0.6350503012478512 1107 56-57 0.7379322679087394 1107 58-59 0.7210605769720999 1107 60-61 0.7565568586971878 1107 62-63 0.8129565686387963 1107 64-65 0.8343726813587722 1107 66-67 0.7405044427917176 1107 68-69 0.7805905439248662 1107 70-71 0.7944621236352276 1107 72-73 0.7588854165375025 1107 74-75 0.811371865617069 1107 76-77 0.6589982755324542 1107 78-79 0.5936739344737454 1107 80-81 0.7227515067883914 1107 82-83 0.8628648900915046 1107 84-85 0.8059794588037796 1107 86-87 0.8425239223643501 1107 88-89 0.686753202440503 1107 90-91 0.8153593469295934 1107 92-93 0.8615783697997017 1107 94-95 0.9408412274523847 1107 96-97 0.7460886034582686 1107 98-99 1.1480133502025573 1107 100-101 1.2014932794231719 1108 1 0.050334793257722055 1108 2 0.015940087681315163 1108 3 -0.047723686617999306 1108 4 0.03150394875974172 1108 5 0.056991355767486596 1108 6 -0.033189976580921154 1108 7 0.011819268800884686 1108 8 -5.579459263245212E-4 1108 9 0.06574399666641995 1108 10-11 -0.08682150185685344 1108 12-13 0.039667561272182184 1108 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2420.0 8 2801.0 9 3635.0 10 4504.0 11 5523.0 12 7080.0 13 8411.0 14 9215.0 15 11028.0 16 13417.0 17 17101.0 18 15588.0 19 11247.0 20 11036.0 21 12219.0 22 13199.0 23 14916.0 24 16873.0 25 19484.0 26 22806.0 27 27154.0 28 32261.0 29 38405.0 30 47384.0 31 58561.0 32 74109.0 33 95563.0 34 129838.0 35 182237.0 36 274454.0 37 401699.0 38 359438.0 39 64000.0 40 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.521692481905376 18.872372532354742 14.703907347617406 22.902027638122473 2 15.10473937430408 20.134449350820535 40.834307478311544 23.926503796563843 3 19.230757795737556 24.633936048441843 29.823744233408817 26.311561922411787 4 12.770436072979019 16.69322434689171 37.7876717183257 32.74866786180358 5 13.806269518485351 37.18942736520158 34.392302588712724 14.61200052760034 6 31.367817032556918 36.09837748566072 18.06801020673853 14.46579527504383 7 27.944829856037366 32.61930300753221 21.309780378177003 18.126086758253415 8 25.862339948188456 34.67838417392814 20.14933624205164 19.309939635831775 9 25.869534179534515 17.152577965587465 20.74944526297212 36.228442591905896 10-11 24.51513592900831 26.590453055381698 27.81823252009345 21.076178495516544 12-13 25.647637378095105 24.00260757808792 28.09769494558664 22.25206009823034 14-15 22.705703987117655 25.35729696785536 26.485567961089252 25.45143108393774 16-17 21.91437640963597 28.549903560516487 27.021741075289707 22.513978954557835 18-19 21.922646713802656 27.35882563029948 29.13337740094763 21.585150254950232 20-21 22.909372454228468 26.326453074034227 29.015655247979062 21.748519223758244 22-23 22.466694451230904 26.218651305535882 29.03766129955444 22.27699294367877 24-25 22.297204903705527 26.392643252648647 29.076809658381524 22.23334218526431 26-27 22.18626626749612 26.47257717167884 29.083476548895504 22.25768001192954 28-29 22.009321069064296 26.784300533382744 29.039410673005563 22.166967724547398 30-31 22.255476092512254 26.425176190878425 28.97873323892229 22.34061447768703 32-33 22.137440697245818 26.429136924461073 29.008410388930944 22.425011989362165 34-35 22.472565024378802 26.56339293256301 28.787792838530667 22.176249204527522 36-37 22.464568337393352 26.337069344347654 28.886558733055796 22.311803585203197 38-39 22.218137825597147 26.637064148916878 28.864808012991684 22.27999001249429 40-41 22.419066488013094 26.56684162925519 28.763324393223154 22.25076748950856 42-43 22.11511118553315 26.605237109602193 28.571531192686912 22.708120512177747 44-45 22.305552342294163 26.58103400742466 28.55879570651031 22.55461794377087 46-47 22.58538106619418 26.75106208480479 28.18696655798323 22.4765902910178 48-49 22.486798239649694 26.829340790076962 28.073435945299046 22.610425024974294 50-51 22.355346229757686 27.01620574037524 28.114472609960444 22.513975419906625 52-53 22.565701649498887 27.15464228572108 27.699141152803758 22.580514911976273 54-55 22.456613640614414 26.882684968767766 27.807401847303304 22.853299543314517 56-57 22.518285884290677 27.162790259848197 27.628622960882687 22.690300894978442 58-59 22.682447176256012 26.94141610908993 27.73020213462447 22.645934580029593 60-61 22.552909528669236 26.94613433471717 28.05921606705837 22.441740069555223 62-63 22.316534351689644 26.943231722301054 28.189233537585913 22.551000388423397 64-65 22.33653209983362 27.10389224679143 27.966868107791846 22.592707545583103 66-67 22.302897265263045 27.086878316841005 27.717294894728234 22.892929523167716 68-69 22.33366341933412 27.00591676678284 27.930430162525226 22.729989651357812 70-71 22.511403053966358 26.882755489377143 27.777403266116202 22.828438190540297 72-73 22.35315012619529 26.902893413098894 27.828036533096263 22.915919927609558 74-75 22.32793096503139 27.367441063102447 27.49321638235301 22.81141158951316 76-77 22.46137494301627 27.019899708639723 27.546900085227836 22.971825263116166 78-79 22.433699172297295 27.01962359310733 27.60300695397129 22.943670280624097 80-81 22.502887226029582 27.15494643356009 27.44234285843185 22.89982348197848 82-83 22.577484927687838 27.070499496660467 27.569057118568857 22.78295845708284 84-85 22.42479297569019 27.134676534734602 27.464102078814406 22.976428410760803 86-87 22.431479568563546 27.103681372715414 27.573314885628108 22.89152417309293 88-89 22.491828905243054 26.94254473254642 27.376444795020692 23.189181567189827 90-91 22.423705053255485 26.878129058653766 27.538442056828156 23.159723831262596 92-93 22.432274371809484 26.877434702879516 27.628562224314813 23.061728700996184 94-95 22.33554422720861 26.95211260348237 27.592887022447666 23.119456146861356 96-97 22.141180824990467 26.93483411177503 27.741030991583514 23.18295407165099 98-99 22.121695018823658 27.029004019959295 27.479939231041232 23.36936173017581 100-101 22.18832831741551 26.865414509152718 27.53219569788682 23.414061475544955 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 123.0 1 94.0 2 96.5 3 124.5 4 132.0 5 160.5 6 238.5 7 508.5 8 897.0 9 1156.5 10 1191.5 11 1203.0 12 1353.0 13 1527.5 14 1690.5 15 1800.5 16 1906.5 17 2136.5 18 2349.0 19 2527.5 20 2848.0 21 3354.0 22 4089.0 23 5165.0 24 6489.5 25 8424.0 26 11094.5 27 14083.5 28 16935.5 29 19728.5 30 23308.0 31 27403.0 32 31251.0 33 34951.5 34 38966.5 35 42955.5 36 47438.0 37 51710.0 38 54958.0 39 57702.5 40 60356.0 41 63164.5 42 64980.0 43 67332.0 44 70887.0 45 73721.0 46 76070.5 47 79319.5 48 84197.0 49 84108.0 50 78381.0 51 74307.5 52 72545.5 53 71398.5 54 69340.0 55 64188.0 56 56995.5 57 50927.0 58 46253.5 59 40954.5 60 34433.0 61 28296.0 62 22398.5 63 16868.5 64 12529.0 65 9082.5 66 6472.0 67 4945.5 68 3554.5 69 2303.0 70 1546.5 71 985.0 72 612.5 73 398.5 74 253.0 75 139.5 76 98.0 77 62.0 78 34.5 79 24.0 80 19.5 81 16.0 82 13.0 83 12.0 84 8.0 85 7.0 86 7.5 87 6.5 88 7.0 89 4.5 90 3.0 91 2.5 92 3.0 93 2.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035668549999133055 2 0.028534839999306442 3 0.024522128124403977 4 0.047756225276617034 5 0.09447211784492603 6 0.11245501180282227 7 0.05533579215143281 8 0.08223582360911233 9 0.06999952937329862 10-11 0.07170864739409041 12-13 0.06873626822749598 14-15 0.0619493357971054 16-17 0.04946534329740883 18-19 0.060958542741573925 20-21 0.05679721190834173 22-23 0.033587884582516964 24-25 0.009561152985878722 26-27 0.0032696170832538633 28-29 0.007282328958156332 30-31 0.005275973020705098 32-33 0.006390615208178005 34-35 0.006464924687342867 36-37 0.0060438376387419905 38-39 0.0031209981249241425 40-41 0.007604336701204061 42-43 0.003319156736030437 44-45 0.0020063559374512343 46-47 0.0015604990624620712 48-49 4.7062670137745007E-4 50-51 0.002031125763839521 52-53 0.006118147117906851 54-55 0.011765667534436251 56-57 0.007752955659533782 58-59 0.005127354062375376 60-61 0.002229284374945816 62-63 0.008372201319240954 64-65 0.0023035938541106768 66-67 0.023531335068872502 68-69 0.04602233742943696 70-71 0.056623823123623726 72-73 0.004285179965173624 74-75 0.015035284617690114 76-77 0.023035938541106766 78-79 0.026231246145195772 80-81 0.07482964551901455 82-83 0.027915594339599274 84-85 0.011047342569175934 86-87 0.03113567177007656 88-89 0.04728559857523959 90-91 0.04485815558918748 92-93 0.043842592707267716 94-95 0.012186754583037128 96-97 0.01701687072875306 98-99 0.038566619686562616 100-101 0.07463148690790827 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2018585.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.339263916238664 #Duplication Level Percentage of deduplicated Percentage of total 1 78.80999546996541 49.91767102309713 2 12.593829591937372 15.953677924797146 3 3.867052668018571 7.348088085528694 4 1.67386762847916 4.240861739243602 5 0.8813802089761777 2.7912986833445856 6 0.5387395441485998 2.0474019701365362 7 0.3313700632817644 1.4692115124501068 8 0.24425124526865155 1.2376555268752847 9 0.1641044528927954 0.9354829726446049 >10 0.839534615689958 9.218307683929007 >50 0.03792811317713833 1.6580783930220766 >100 0.01669340179514378 1.8450825187742728 >500 6.264981845983418E-4 0.2709780058281651 >1k 5.481859115235442E-4 0.7759306141625402 >5k 7.831227307479203E-5 0.29027334616628425 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5852 0.2899060480485092 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4413 0.2186184877030197 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2844 0.14089077249657556 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2783 0.13786885367720458 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.953965277657369E-5 0.0 0.0 0.0 0.0 6 4.953965277657369E-5 0.0 0.0 0.0 0.0 7 4.953965277657369E-5 0.0 0.0 4.953965277657369E-5 0.0 8 4.953965277657369E-5 0.0 0.0 4.953965277657369E-5 0.0 9 4.953965277657369E-5 0.0 0.0 4.953965277657369E-5 0.0 10-11 4.953965277657369E-5 0.0 0.0 4.953965277657369E-5 0.0 12-13 4.953965277657369E-5 0.0 0.0 4.953965277657369E-5 0.0 14-15 4.953965277657369E-5 0.0 0.0 4.953965277657369E-5 0.0 16-17 4.953965277657369E-5 0.0 0.0 4.953965277657369E-5 0.0 18-19 4.953965277657369E-5 0.0 0.0 4.953965277657369E-5 0.0 20-21 4.953965277657369E-5 0.0 0.0 7.430947916486054E-5 0.0 22-23 4.953965277657369E-5 0.0 0.0 1.9815861110629475E-4 0.0 24-25 4.953965277657369E-5 0.0 0.0 3.467775694360158E-4 0.0 26-27 4.953965277657369E-5 0.0 0.0 7.183249652603185E-4 0.0 28-29 4.953965277657369E-5 0.0 0.0 0.0014118801041323502 0.0 30-31 4.953965277657369E-5 0.0 0.0 0.002774220555488127 0.0 32-33 4.953965277657369E-5 0.0 0.0 0.003641164479078166 0.0 34-35 4.953965277657369E-5 0.0 0.0 0.004458568749891633 0.0 36-37 4.953965277657369E-5 0.0 0.0 0.005994297985965416 0.0 38-39 9.907930555314738E-5 0.0 0.0 0.007554797048427488 0.0 40-41 9.907930555314738E-5 0.0 0.0 0.010700564999739917 0.0 42-43 9.907930555314738E-5 0.0 0.0 0.014490348437147804 0.0 44-45 1.4861895832972107E-4 0.0 0.0 0.02206991531196358 0.0 46-47 1.4861895832972107E-4 0.0 0.0 0.03856661968656262 0.0 48-49 1.4861895832972107E-4 0.0 0.0 0.05110015183903576 0.0 50-51 1.4861895832972107E-4 0.0 0.0 0.06668037263726818 0.0 52-53 1.4861895832972107E-4 0.0 0.0 0.08701640010205168 0.0 54-55 1.4861895832972107E-4 0.0 0.0 0.1080707525320955 0.0 56-57 1.4861895832972107E-4 0.0 0.0 0.13056175489265995 0.0 58-59 1.4861895832972107E-4 0.0 0.0 0.16147449822524196 0.0 60-61 1.4861895832972107E-4 0.0 0.0 0.1990007852034965 0.0 62-63 1.4861895832972107E-4 0.0 0.0 0.24296722704270568 0.0 64-65 1.4861895832972107E-4 0.0 0.0 0.27766975381269554 0.0 66-67 1.4861895832972107E-4 0.0 0.0 0.33805859054733883 0.0 68-69 1.4861895832972107E-4 0.0 0.0 0.4149441316565812 0.0 70-71 1.9815861110629475E-4 0.0 0.0 0.4607930803013002 0.0 72-73 1.9815861110629475E-4 0.0 0.0 0.5486268846741653 0.0 74-75 1.9815861110629475E-4 0.0 0.0 0.619493357971054 0.0 76-77 1.9815861110629475E-4 0.0 0.0 0.6734915794975194 0.0 78-79 1.9815861110629475E-4 0.0 0.0 0.7437635769610891 0.0 80-81 2.4769826388286844E-4 0.0 0.0 0.819831713799518 0.0 82-83 2.4769826388286844E-4 0.0 0.0 0.9158147910541294 0.0 84-85 2.4769826388286844E-4 0.0 0.0 1.065424542439382 0.0 86-87 2.4769826388286844E-4 0.0 0.0 1.289591471253378 0.0 88-89 2.4769826388286844E-4 0.0 0.0 1.561861402913427 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10355 0.0 29.928417 1 GGTATCA 7235 0.0 27.198696 1 TATCAAC 12200 0.0 25.249018 2 TCAACGC 12210 0.0 25.190657 4 ATCAACG 12200 0.0 25.17109 3 CAACGCA 12250 0.0 25.108402 5 AACGCAG 12515 0.0 24.612286 6 GTACATG 8590 0.0 24.12568 1 TACATGG 8465 0.0 23.642014 2 ACATGGG 8585 0.0 22.70246 3 TAGGTCG 105 0.0021863142 22.637663 5 GAGTACT 6110 0.0 22.478786 12-13 ACGCAGA 13840 0.0 22.149096 7 CGCAGAG 13975 0.0 21.83311 8 AGAGTAC 10475 0.0 20.82374 10-11 GCAGAGT 14755 0.0 20.38854 9 GTACTTT 6650 0.0 20.369097 14-15 CATGGGG 4255 0.0 20.333996 4 AGTACTT 6640 0.0 19.25309 12-13 CGTATGA 175 9.882694E-5 19.014694 2 >>END_MODULE