FastQCFastQC Report
Thu 26 May 2016
SRR936984_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936984_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018585
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60220.29832778902052676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48690.24120856936913732No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34440.1706145641625198No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30720.15218581332963438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA114450.027.3959311
GGTATCA83400.023.753521
ATCAACG134500.022.9536723
TCAACGC134950.022.8771324
CAACGCA135500.022.7842735
TATCAAC136350.022.746742
AACGCAG138200.022.4100936
GTACATG80000.022.1501561
CACGCGT1100.002877707421.591436
TACATGG80950.021.2399182
TACCGTC1604.9907518E-520.7817557
GAGTACT68500.020.69889512-13
AGAGTAC114150.020.20357510-11
ACATGGG80150.020.0889533
ACGCAGA154000.019.9258067
AGTACTT72850.019.8215412-13
ACCGTCG1200.004763373619.7926378
CGCAGAG154650.019.7196878
GTACTTT73150.019.41557514-15
GCAGAGT163650.018.3449279
Produced by FastQC (version 0.11.5)