##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936984_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2018585 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.175704268088786 33.0 31.0 34.0 31.0 34.0 2 32.31115608210702 33.0 31.0 34.0 31.0 34.0 3 32.443847546672544 34.0 31.0 34.0 31.0 34.0 4 36.097507907767074 37.0 35.0 37.0 35.0 37.0 5 35.93446052556617 37.0 35.0 37.0 35.0 37.0 6 36.01710901448292 37.0 35.0 37.0 35.0 37.0 7 35.979145292370646 37.0 35.0 37.0 35.0 37.0 8 35.92408196830949 37.0 35.0 37.0 35.0 37.0 9 37.633674578974876 39.0 37.0 39.0 35.0 39.0 10-11 37.54632601550095 39.0 37.0 39.0 35.0 39.0 12-13 37.45214593390915 39.0 37.0 39.0 35.0 39.0 14-15 38.67778270422103 40.0 38.0 41.0 34.5 41.0 16-17 38.66727633466017 40.0 38.0 41.0 34.5 41.0 18-19 38.77188773323888 40.0 38.0 41.0 35.0 41.0 20-21 38.766261762571304 40.0 38.0 41.0 34.5 41.0 22-23 38.67482815932944 40.0 38.0 41.0 34.0 41.0 24-25 38.58540933376598 40.0 38.0 41.0 34.0 41.0 26-27 38.39301738594114 40.0 38.0 41.0 34.0 41.0 28-29 38.25196560957304 40.0 38.0 41.0 34.0 41.0 30-31 38.27852134044393 40.0 38.0 41.0 34.0 41.0 32-33 38.173563411994046 40.0 38.0 41.0 34.0 41.0 34-35 38.07054322706252 40.0 38.0 41.0 33.0 41.0 36-37 37.94114664480317 40.0 37.0 41.0 33.0 41.0 38-39 37.792225494591506 40.0 37.0 41.0 33.0 41.0 40-41 37.48295092849694 40.0 37.0 41.0 32.5 41.0 42-43 37.30632547056477 40.0 37.0 41.0 32.0 41.0 44-45 37.11491738024408 40.0 36.5 41.0 31.5 41.0 46-47 36.79427470232861 39.0 36.0 41.0 31.0 41.0 48-49 36.463927454132474 39.0 36.0 40.5 30.0 41.0 50-51 36.199411716623274 39.0 35.0 40.0 30.0 41.0 52-53 35.94639314173047 39.0 35.0 40.0 29.0 41.0 54-55 35.563566310063734 38.0 35.0 40.0 28.0 41.0 56-57 35.370656920565644 38.0 34.5 40.0 28.0 41.0 58-59 35.05670506815418 38.0 34.0 40.0 27.5 41.0 60-61 34.6727819239715 37.5 34.0 40.0 26.5 41.0 62-63 34.29353705689877 37.0 33.5 39.5 26.0 41.0 64-65 33.98264303955494 36.5 33.0 39.0 26.0 40.0 66-67 33.64802423479814 36.0 33.0 39.0 26.0 40.0 68-69 33.1903697391985 35.5 32.0 39.0 25.0 40.0 70-71 32.74152785243128 35.0 32.0 38.0 24.0 40.0 72-73 32.325201069065706 35.0 31.0 37.5 23.0 39.5 74-75 31.840787482320536 35.0 31.0 37.0 22.0 39.0 76-77 30.394221942598406 33.5 29.5 35.0 20.5 37.5 78-79 31.085641922435766 34.0 31.0 36.0 21.0 38.0 80-81 30.958045858856575 34.0 31.0 35.5 20.0 37.0 82-83 30.607834200690085 34.0 30.5 35.0 19.5 37.0 84-85 30.173201029433983 34.0 30.0 35.0 18.0 36.0 86-87 29.803587909352345 34.0 30.0 35.0 16.0 36.0 88-89 29.424801779464328 34.0 29.0 35.0 9.0 35.5 90-91 28.975749596871076 34.0 29.0 35.0 4.5 35.0 92-93 28.492459321752612 33.0 29.0 35.0 2.0 35.0 94-95 27.939606704696608 33.0 28.0 34.5 2.0 35.0 96-97 27.168150461833413 33.0 26.5 34.0 2.0 35.0 98-99 26.324013355890386 32.0 25.0 34.0 2.0 35.0 100-101 23.170298253479544 29.0 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10443239566728124 1101 2 0.2046840409088304 1101 3 0.11381861312705865 1101 4 0.07976065945437227 1101 5 -0.04704755653735759 1101 6 0.03951412757846384 1101 7 0.07225925844962688 1101 8 0.11446008463891388 1101 9 0.03258984567963097 1101 10-11 0.15860457322830968 1101 12-13 0.20405288834204072 1101 14-15 0.34163152444674694 1101 16-17 0.36059568188611024 1101 18-19 0.10737432389235835 1101 20-21 -0.04174703880601527 1101 22-23 0.047479634720900776 1101 24-25 0.09550857139268487 1101 26-27 0.07308182997998358 1101 28-29 0.1820789344241689 1101 30-31 0.18633708738533272 1101 32-33 0.18474769499668042 1101 34-35 0.04338035342041735 1101 36-37 0.11057750027390512 1101 38-39 0.18606335878017433 1101 40-41 0.23757855096224034 1101 42-43 0.19757568447598572 1101 44-45 -0.018877821645958193 1101 46-47 0.08217056269846523 1101 48-49 0.12307006263579723 1101 50-51 0.1462334826146403 1101 52-53 0.25659454954887906 1101 54-55 0.17759371365652754 1101 56-57 0.22701436838575972 1101 58-59 0.21520378841940158 1101 60-61 0.23306842297175479 1101 62-63 0.19023462762670817 1101 64-65 0.2289171954665079 1101 66-67 0.20589223333672635 1101 68-69 -0.05232970943367121 1101 70-71 0.0494934151030364 1101 72-73 0.242180741832712 1101 74-75 0.016879528650942888 1101 76-77 0.07513462966658935 1101 78-79 -0.023507192799225862 1101 80-81 0.08810580049467376 1101 82-83 0.3301317959320542 1101 84-85 0.18726878533914615 1101 86-87 -0.04463079081322263 1101 88-89 0.20788724676925696 1101 90-91 0.16081269952781696 1101 92-93 -0.07841733310236876 1101 94-95 -0.20883436468364636 1101 96-97 -0.1971902120725595 1101 98-99 -0.03560648617971651 1101 100-101 0.36900098041762774 1102 1 -0.08337541869349963 1102 2 -0.042480754795768405 1102 3 0.042154809590883247 1102 4 0.06636072632878864 1102 5 -0.05240660200713876 1102 6 0.05077738231394591 1102 7 0.11939922674923054 1102 8 0.11769117285487596 1102 9 0.221044508574316 1102 10-11 0.2477010004200011 1102 12-13 0.2256248972799071 1102 14-15 0.19575131500588583 1102 16-17 0.1313715624147065 1102 18-19 0.12424044480144403 1102 20-21 0.08416650021995764 1102 22-23 0.018740521760662432 1102 24-25 0.047818150948558014 1102 26-27 0.10537953765714292 1102 28-29 -0.06722476634362806 1102 30-31 -0.07302440297150525 1102 32-33 -0.17309453630790017 1102 34-35 0.17036223164144104 1102 36-37 0.11787653467712289 1102 38-39 0.21345013894778475 1102 40-41 0.34511285193347874 1102 42-43 0.40307822047108033 1102 44-45 0.28238334743906535 1102 46-47 0.13765424619469968 1102 48-49 0.23831384808396905 1102 50-51 0.1574409777447201 1102 52-53 0.34390759714810315 1102 54-55 0.4137852787961833 1102 56-57 0.2677607232291308 1102 58-59 0.10106035059201446 1102 60-61 0.09069420309594989 1102 62-63 0.141087777422797 1102 64-65 0.21228682357127582 1102 66-67 0.3494002937077667 1102 68-69 0.39525236480880466 1102 70-71 0.08123714506994162 1102 72-73 0.0712137199745797 1102 74-75 0.07729269526584304 1102 76-77 0.13429306599283208 1102 78-79 -0.15837399878140346 1102 80-81 0.21512497549002418 1102 82-83 0.391261672949188 1102 84-85 0.22281502350484672 1102 86-87 0.05928952694787171 1102 88-89 0.06621693392539996 1102 90-91 -0.0133067347636171 1102 92-93 -0.022257854322010218 1102 94-95 0.0891580482988985 1102 96-97 0.3224705691260077 1102 98-99 0.1779599464264976 1102 100-101 -0.08802190941066712 1103 1 -0.012096063951041458 1103 2 0.1409182176705599 1103 3 0.09317245756137993 1103 4 0.06997045617752917 1103 5 -0.02997909353729966 1103 6 -0.022917270426312086 1103 7 -0.01802634079148646 1103 8 0.09102005460449192 1103 9 0.13691718072676196 1103 10-11 0.12538598255331124 1103 12-13 0.08650327996450358 1103 14-15 0.15445877208454561 1103 16-17 0.1240346873685354 1103 18-19 0.0414254167955761 1103 20-21 -0.023345583512302426 1103 22-23 0.025352535822257494 1103 24-25 0.04450182367893518 1103 26-27 0.02575952885054278 1103 28-29 -0.06479815229448604 1103 30-31 0.12223523975269046 1103 32-33 0.0580244057119117 1103 34-35 0.0600581611944051 1103 36-37 0.0807614252681077 1103 38-39 0.09009024216466344 1103 40-41 0.11705666710488316 1103 42-43 -0.07479573066675727 1103 44-45 0.015511839119866977 1103 46-47 0.09848350816363904 1103 48-49 0.14959718245535925 1103 50-51 -0.11830548331865032 1103 52-53 -0.06079070062808967 1103 54-55 0.08752269715765948 1103 56-57 0.0019586270256795046 1103 58-59 -0.11483270855853078 1103 60-61 -0.3301165222756097 1103 62-63 -0.06362248820928329 1103 64-65 -0.311497977360375 1103 66-67 -0.21003396166346278 1103 68-69 -0.2140702871377016 1103 70-71 -0.17114511005795663 1103 72-73 -0.058141239751137164 1103 74-75 -0.15941900143935683 1103 76-77 -0.16242584039499874 1103 78-79 -0.1926270524857543 1103 80-81 -0.13987349639040048 1103 82-83 -0.008515953062737225 1103 84-85 -0.17403428000577392 1103 86-87 -0.2648016402313047 1103 88-89 -0.0973088797077466 1103 90-91 -0.2280681887802558 1103 92-93 -0.3518034487750086 1103 94-95 -0.16573637022625576 1103 96-97 -4.993012731446811E-5 1103 98-99 -0.14738313814441995 1103 100-101 0.572921038136446 1104 1 -9.156244746719722E-4 1104 2 0.09992134637916195 1104 3 -0.018849987034698756 1104 4 -0.008888798172613122 1104 5 -0.21623358238645807 1104 6 0.01390267680738333 1104 7 0.07946750477388775 1104 8 0.0382236224251713 1104 9 -0.04207318374747615 1104 10-11 0.029985327808333295 1104 12-13 0.053019495165848696 1104 14-15 0.08826549831764652 1104 16-17 0.08015596791127422 1104 18-19 0.09838353581541526 1104 20-21 0.2129505537305434 1104 22-23 0.14178221413833114 1104 24-25 0.07341742070463653 1104 26-27 0.03517656693166771 1104 28-29 -0.032189369072099794 1104 30-31 -0.04558782077724288 1104 32-33 -0.056548346220587575 1104 34-35 0.06509528746224902 1104 36-37 -0.08998779395587064 1104 38-39 -0.07674371280042891 1104 40-41 0.18741977174218505 1104 42-43 0.01596221405332443 1104 44-45 0.11689196320335782 1104 46-47 -0.10247839809279924 1104 48-49 0.12251390458315825 1104 50-51 0.11033001162119405 1104 52-53 0.016014594357429246 1104 54-55 0.07877653448443311 1104 56-57 -0.03352885183487331 1104 58-59 -0.3175397140355187 1104 60-61 -0.18615520659269436 1104 62-63 0.03756506897406098 1104 64-65 -0.019198849419595376 1104 66-67 -0.15008742152758003 1104 68-69 -0.06124887993340877 1104 70-71 -0.042843450287151086 1104 72-73 -0.11459460405864519 1104 74-75 0.03384338133915321 1104 76-77 0.18132429096032254 1104 78-79 0.18561882505886018 1104 80-81 0.05095916323609018 1104 82-83 0.2697984744452384 1104 84-85 0.09756552621885461 1104 86-87 0.05693675461368031 1104 88-89 0.04804121199578404 1104 90-91 0.1259524399598675 1104 92-93 0.04830862358461019 1104 94-95 0.0727757312767281 1104 96-97 0.24702382360843345 1104 98-99 -0.07500521802807114 1104 100-101 0.4660364255540763 1105 1 -0.0036711606241155437 1105 2 0.08115577150272202 1105 3 0.0511339439215206 1105 4 0.036029784683272226 1105 5 -0.0017147981266489865 1105 6 0.021006885638080064 1105 7 0.060605554971459696 1105 8 0.05421005912946697 1105 9 0.18247842699223327 1105 10-11 0.04157490436838174 1105 12-13 0.10537959909342476 1105 14-15 0.060206627005705116 1105 16-17 -0.1679573453996781 1105 18-19 0.011676588363343399 1105 20-21 0.09644998629858037 1105 22-23 0.03665978847978124 1105 24-25 -0.07068767910242002 1105 26-27 -0.047484600463704396 1105 28-29 -0.09501401981133739 1105 30-31 0.004942142973980879 1105 32-33 0.13269168831704548 1105 34-35 -0.012442274173452006 1105 36-37 -0.018371389763359502 1105 38-39 -0.003456482084757795 1105 40-41 0.04299604462876516 1105 42-43 0.05139475062170362 1105 44-45 -0.17085406329778152 1105 46-47 -0.0371637339594173 1105 48-49 -0.09839611073848431 1105 50-51 -0.11297954267427457 1105 52-53 -0.05631970469199388 1105 54-55 0.07390960072154229 1105 56-57 -0.2084316410039051 1105 58-59 -0.1711894718197584 1105 60-61 -0.2767535375363295 1105 62-63 -0.32647351246569656 1105 64-65 -0.20819597980700166 1105 66-67 -0.22112852641693337 1105 68-69 -0.30780052607614294 1105 70-71 -0.4067364859203053 1105 72-73 -0.2981288496101513 1105 74-75 -0.30602181177456345 1105 76-77 -0.31218711963423473 1105 78-79 -0.18159226159793107 1105 80-81 -0.26135361863598305 1105 82-83 -0.24718514771784683 1105 84-85 -0.5542623520969805 1105 86-87 -0.33356067768293585 1105 88-89 -0.16059673494293847 1105 90-91 -0.2294497680093528 1105 92-93 -0.24464951626130826 1105 94-95 -0.2182254075208654 1105 96-97 0.15103734756743492 1105 98-99 0.06687539114013674 1105 100-101 0.15891805550976557 1106 1 -0.029948514100851753 1106 2 0.031691634771263466 1106 3 0.07108860411965168 1106 4 -0.02369049833660597 1106 5 -0.025379858383118403 1106 6 0.005178705042396814 1106 7 0.09368816820836656 1106 8 0.06817017125646885 1106 9 0.1256106480634429 1106 10-11 0.11424512701383094 1106 12-13 0.18061600305090053 1106 14-15 0.23498148293280252 1106 16-17 0.04076230921140933 1106 18-19 0.0497564456094608 1106 20-21 -0.02235037487562863 1106 22-23 0.09239514473181032 1106 24-25 0.09369055007162075 1106 26-27 0.18648400483337468 1106 28-29 0.033186736423957086 1106 30-31 -0.03655964681147594 1106 32-33 -0.04741070156665472 1106 34-35 -0.1010843749890995 1106 36-37 -0.04312276078834998 1106 38-39 0.08518132925104993 1106 40-41 0.10702618251409746 1106 42-43 0.28324542524964613 1106 44-45 0.026736535878207235 1106 46-47 0.04971217353366342 1106 48-49 -0.007643585843418066 1106 50-51 0.06797538132686753 1106 52-53 -0.02151701004076756 1106 54-55 0.23384071167537002 1106 56-57 0.08589209032881229 1106 58-59 0.21263103918597182 1106 60-61 0.1298687759088324 1106 62-63 0.054700389481084244 1106 64-65 -0.12161604642261636 1106 66-67 -0.009051281464046212 1106 68-69 -0.0675381814893612 1106 70-71 -0.043737110039621996 1106 72-73 -0.09762033177557328 1106 74-75 -0.1161651447432881 1106 76-77 0.02185076076220227 1106 78-79 -0.021982861631819617 1106 80-81 0.07877103364805649 1106 82-83 0.10645136605031169 1106 84-85 0.0816691191731529 1106 86-87 0.021436673563265174 1106 88-89 0.22288169004585612 1106 90-91 0.1865128877791875 1106 92-93 0.3551591729379986 1106 94-95 0.4472981127694453 1106 96-97 0.22336762893586481 1106 98-99 0.2813987119696364 1106 100-101 0.7237556879742648 1107 1 0.18832287679767745 1107 2 0.25806152766670465 1107 3 0.20073920710629523 1107 4 0.10837896163132399 1107 5 -6.452361444573285E-4 1107 6 0.05429018349300918 1107 7 0.09729163311547495 1107 8 0.10465333996130255 1107 9 0.19017232672963758 1107 10-11 0.17861020931204052 1107 12-13 0.19564490971485782 1107 14-15 0.24957477228307567 1107 16-17 0.32793449460856294 1107 18-19 0.2510554905534832 1107 20-21 0.31100391561595586 1107 22-23 0.10582602252037532 1107 24-25 0.2350737356369379 1107 26-27 0.22700188209120142 1107 28-29 0.09998510880721767 1107 30-31 0.11913571060574668 1107 32-33 0.2021121859463335 1107 34-35 0.2920980828416333 1107 36-37 0.32645304373143347 1107 38-39 0.22249875322194157 1107 40-41 0.34691683207155677 1107 42-43 0.39386696499501994 1107 44-45 0.3323897639402702 1107 46-47 0.30495461578102123 1107 48-49 0.4482301516153626 1107 50-51 0.39070712612947034 1107 52-53 0.5895945991825187 1107 54-55 0.6745252475450272 1107 56-57 0.4543559991500885 1107 58-59 0.46491152425197413 1107 60-61 0.35495816821174486 1107 62-63 0.3637124850780964 1107 64-65 0.5446589947390592 1107 66-67 0.5294580981945956 1107 68-69 0.25215889390458557 1107 70-71 0.21493308501065655 1107 72-73 0.44089976336920245 1107 74-75 0.49066661184634697 1107 76-77 0.265775572164177 1107 78-79 0.4689327023056258 1107 80-81 0.5441686514424084 1107 82-83 0.7855933005367 1107 84-85 0.6803982187814732 1107 86-87 0.7138324616336931 1107 88-89 0.593648772334884 1107 90-91 0.7355329565726123 1107 92-93 0.7319167586821145 1107 94-95 0.709802265020322 1107 96-97 0.563550855001349 1107 98-99 0.8221838203305332 1107 100-101 1.419501049535544 1108 1 0.07392304033678698 1108 2 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35.0 6 54.0 7 170.0 8 445.0 9 927.0 10 1505.0 11 2471.0 12 3837.0 13 5261.0 14 6939.0 15 9456.0 16 12369.0 17 16587.0 18 15175.0 19 10123.0 20 9213.0 21 9571.0 22 10431.0 23 11567.0 24 13634.0 25 16270.0 26 20180.0 27 25013.0 28 31473.0 29 40299.0 30 49883.0 31 63011.0 32 81070.0 33 107107.0 34 145571.0 35 209044.0 36 323713.0 37 463352.0 38 287763.0 39 15057.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.82245061576074 19.525417782785652 15.085023244014085 22.567108357439523 2 15.588854997153852 20.85167392528879 39.73759969561842 23.821871381938937 3 19.503908688285826 24.754381793141714 29.983156476334848 25.758553042237615 4 13.346676013147823 17.32847514471771 37.08300616520979 32.241842676924676 5 13.890880159006565 37.63233001392596 34.14410366340307 14.332686163664404 6 31.761109886380805 36.47619495834954 17.435134017145675 14.327561138123984 7 28.250693434037938 32.84363192486588 20.84790424145326 18.057770399642916 8 26.312837953318784 34.95805229901144 19.67739778111895 19.051711966550826 9 26.161804512470127 17.062802439730028 20.41881833998486 36.35657470781498 10-11 24.758481807801008 26.873230505527385 27.410339420931003 20.957948265740605 12-13 25.849963846083153 24.175439265754697 27.889817625226442 22.084779262935708 14-15 22.839989507135076 25.573930729752004 26.300529423989772 25.28555033912315 16-17 22.07808935467171 28.77820354357136 26.80959682153588 22.334110280221044 18-19 22.033698046244528 27.554278416347383 29.020164730151087 21.391858807257005 20-21 23.007389657353034 26.482111421260008 29.038383465081253 21.47211545630571 22-23 22.63308615113362 26.36640680210537 29.03656409063628 21.963942956124725 24-25 22.525855536947788 26.53289715028673 29.087153960114865 21.854093352650626 26-27 22.402805533785596 26.611601609446634 29.101022400873482 21.884570455894288 28-29 22.21156568455058 26.989123808849126 28.972891138761415 21.826419367838884 30-31 22.392423998103624 26.65739284136978 28.903149707133885 22.04703345339271 32-33 22.362067261522746 26.6802190744153 28.90990142344867 22.047812240613283 34-35 22.66837908733098 26.72025205775333 28.769757032772958 21.841611822142738 36-37 22.672853614511997 26.57074965426924 28.87524983893145 21.881146892287312 38-39 22.4109639535552 26.82295743748997 28.863067505088196 21.903011103866636 40-41 22.694930934932817 26.683898358613224 28.64063252777031 21.980538178683645 42-43 22.42759160501044 26.87590564677732 28.409801915698374 22.286700832513866 44-45 22.63062556057755 26.81614740765162 28.360635312552407 22.192591719218424 46-47 22.88655886811521 27.112177867609905 27.9501679398389 22.05109532443599 48-49 22.859272356085345 27.120948922846143 27.81926038729634 22.200518333772173 50-51 22.682383516331356 27.336701913819066 27.854027298233813 22.126887271615768 52-53 22.952381801635507 27.41931568624828 27.43271621543295 22.195586296683256 54-55 22.727058806044827 27.232293908851997 27.581870468669884 22.458776816433293 56-57 22.83097309095809 27.505476627606168 27.39111394258731 22.272436338848436 58-59 23.007540698793385 27.274774166685038 27.419306497465172 22.298378637056405 60-61 22.890414297802185 27.271459484738536 27.790173927000993 22.04795229045829 62-63 22.626058662904295 27.303372066755706 27.91479076421888 22.155778506121123 64-65 22.673218130350907 27.431236382767704 27.686440283327602 22.20910520355378 66-67 22.746979380848313 27.33673026816564 27.471874474415113 22.444415876570933 68-69 22.728374985912144 27.322638534714333 27.62733254647754 22.32165393289598 70-71 22.872803807233655 27.219526017937874 27.47827245757349 22.42939771725499 72-73 22.819091346166946 27.119169693341284 27.511326653223218 22.55041230726855 74-75 22.716676537753003 27.684718740920022 27.153736038977044 22.444868682349927 76-77 22.765846140584756 27.45448168343833 27.15791225825245 22.621759917724464 78-79 22.857820136477095 27.382385790410634 27.179272761229985 22.580521311882286 80-81 22.91994614939703 27.488181477027418 27.12482614355124 22.467046230024312 82-83 22.907226775607747 27.454227184557883 27.23650024881309 22.402045791021276 84-85 22.805035914554125 27.47779212807667 27.152705840505593 22.564466116863617 86-87 22.742775303127768 27.45864108894398 27.159049222485415 22.639534385442833 88-89 22.79422729279135 27.40034470578385 26.996932921086746 22.808495080338048 90-91 22.838418731797024 27.2333899909688 27.123088424057386 22.8051028531768 92-93 22.780165313821314 27.30234297787807 27.256295870622242 22.661195837678374 94-95 22.726266171600404 27.380318391348396 27.13792087031262 22.755494566738584 96-97 22.54366372805048 27.321253722603583 27.286452038945193 22.84863051040075 98-99 22.480424299530263 27.48829123926098 26.973348512365657 23.057935948843095 100-101 22.471089907693298 27.497538446676252 26.95120365313091 23.08016799249954 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 179.0 1 135.0 2 112.5 3 144.0 4 146.0 5 147.0 6 230.0 7 503.5 8 898.0 9 1141.5 10 1160.5 11 1162.0 12 1330.0 13 1508.5 14 1732.5 15 1982.0 16 2114.5 17 2230.5 18 2483.0 19 2807.5 20 3087.0 21 3560.5 22 4310.0 23 5470.0 24 6826.5 25 8562.0 26 11017.0 27 14089.5 28 16944.0 29 19808.0 30 23190.0 31 26964.0 32 31083.5 33 34819.5 34 38862.5 35 42873.0 36 47419.5 37 51601.0 38 54620.0 39 57534.0 40 60227.5 41 63077.5 42 65199.5 43 67559.0 44 70725.5 45 73570.5 46 76145.0 47 79513.0 48 84568.0 49 84189.0 50 78347.0 51 74604.0 52 72447.0 53 71068.0 54 69228.5 55 64211.0 56 57164.0 57 50791.5 58 46020.5 59 40909.0 60 34345.5 61 28180.5 62 22523.0 63 17048.5 64 12505.5 65 9102.5 66 6370.5 67 4732.0 68 3455.5 69 2207.5 70 1426.5 71 903.0 72 579.5 73 377.5 74 236.5 75 141.0 76 83.5 77 42.5 78 21.5 79 12.5 80 9.5 81 5.0 82 2.5 83 1.0 84 1.0 85 0.5 86 1.0 87 1.0 88 1.0 89 1.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02135159034670326 2 0.0032696170832538633 3 2.4769826388286844E-4 4 0.0 5 0.002576061944381832 6 0.0 7 3.963172222125895E-4 8 0.0 9 4.953965277657369E-5 10-11 0.0 12-13 0.00651446434011944 14-15 0.0034925455207484453 16-17 0.0 18-19 5.449361805423106E-4 20-21 0.0010155628819197605 22-23 0.0016595783680152184 24-25 6.687853124837448E-4 26-27 1.4861895832972107E-4 28-29 7.430947916486054E-5 30-31 2.4769826388286844E-5 32-33 7.430947916486054E-5 34-35 0.0 36-37 0.0017091180207917923 38-39 0.009362994374772428 40-41 8.669439235900395E-4 42-43 0.0 44-45 9.660232291431868E-4 46-47 2.4769826388286844E-4 48-49 7.430947916486054E-5 50-51 0.0014614197569089238 52-53 3.963172222125895E-4 54-55 0.0 56-57 3.467775694360158E-4 58-59 2.724680902711553E-4 60-61 0.0018329671527332267 62-63 4.953965277657369E-5 64-65 1.4861895832972107E-4 66-67 2.4769826388286844E-5 68-69 1.2384913194143422E-4 70-71 3.22007743047729E-4 72-73 2.724680902711553E-4 74-75 0.003145767951312429 76-77 9.907930555314738E-5 78-79 2.2292843749458158E-4 80-81 0.0018577369791215135 82-83 7.430947916486054E-5 84-85 0.0021797447221692423 86-87 2.724680902711553E-4 88-89 0.002576061944381832 90-91 0.0013871102777440632 92-93 0.0 94-95 0.0 96-97 2.2292843749458158E-4 98-99 5.449361805423106E-4 100-101 0.0021054352430043815 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2018585.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.82650403759817 #Duplication Level Percentage of deduplicated Percentage of total 1 77.62398440992926 47.99219585534949 2 13.214814620927804 16.340515790338085 3 4.114449505602001 7.631460869117877 4 1.7902476455493768 4.427390131434366 5 0.9527076288735513 2.94512910316006 6 0.5594475211227062 2.0753210654110377 7 0.3672094880452994 1.5892295226693942 8 0.24247671388683986 1.1993190024118594 9 0.18155851197578177 1.0102615266357888 >10 0.8914606475309493 9.571895101792292 >50 0.0416024200827389 1.7301278696005344 >100 0.0185989014308882 2.010881295134268 >500 8.011028015029944E-4 0.35408557759474596 >1k 5.607719610520773E-4 0.8239213293171714 >5k 8.011028015029676E-5 0.29826596003294303 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6022 0.29832778902052676 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4869 0.24120856936913732 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3444 0.1706145641625198 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3072 0.15218581332963438 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.4769826388286844E-5 0.0 12-13 0.0 0.0 0.0 4.953965277657369E-5 0.0 14-15 0.0 0.0 0.0 4.953965277657369E-5 0.0 16-17 0.0 0.0 0.0 7.430947916486054E-5 0.0 18-19 0.0 0.0 0.0 9.907930555314738E-5 0.0 20-21 0.0 0.0 0.0 1.2384913194143422E-4 0.0 22-23 4.953965277657369E-5 0.0 0.0 2.2292843749458158E-4 0.0 24-25 4.953965277657369E-5 0.0 0.0 3.963172222125895E-4 0.0 26-27 4.953965277657369E-5 0.0 0.0 7.183249652603185E-4 0.0 28-29 7.430947916486054E-5 0.0 0.0 0.0014118801041323502 0.0 30-31 9.907930555314738E-5 0.0 0.0 0.002724680902711553 0.0 32-33 9.907930555314738E-5 0.0 0.0 0.0035668549999133053 0.0 34-35 9.907930555314738E-5 0.0 0.0 0.004508108402668206 0.0 36-37 9.907930555314738E-5 0.0 0.0 0.006093377291518563 0.0 38-39 9.907930555314738E-5 0.0 0.0 0.007678646180368922 0.0 40-41 9.907930555314738E-5 0.0 0.0 0.010799644305293064 0.0 42-43 9.907930555314738E-5 0.0 0.0 0.014564657916312665 0.0 44-45 9.907930555314738E-5 0.0 0.0 0.022243304096681585 0.0 46-47 9.907930555314738E-5 0.0 0.0 0.0387895481240572 0.0 48-49 9.907930555314738E-5 0.0 0.0 0.051075382012647476 0.0 50-51 9.907930555314738E-5 0.0 0.0 0.06648221402616189 0.0 52-53 9.907930555314738E-5 0.0 0.0 0.08676870183816882 0.0 54-55 9.907930555314738E-5 0.0 0.0 0.10772397496265948 0.0 56-57 9.907930555314738E-5 0.0 0.0 0.1303140566287771 0.0 58-59 9.907930555314738E-5 0.0 0.0 0.16117726030858248 0.0 60-61 9.907930555314738E-5 0.0 0.0 0.19895124555071994 0.0 62-63 9.907930555314738E-5 0.0 0.0 0.2428681477371525 0.0 64-65 1.2384913194143422E-4 0.0 0.0 0.27766975381269554 0.0 66-67 1.4861895832972107E-4 0.0 0.0 0.3378356621098443 0.0 68-69 1.9815861110629475E-4 0.0 0.0 0.4149689014829695 0.0 70-71 1.9815861110629475E-4 0.0 0.0 0.46123893717628933 0.0 72-73 1.9815861110629475E-4 0.0 0.0 0.5490232018963779 0.0 74-75 1.9815861110629475E-4 0.0 0.0 0.6202116829363142 0.0 76-77 1.9815861110629475E-4 0.0 0.0 0.6743585234211094 2.4769826388286844E-5 78-79 1.9815861110629475E-4 0.0 0.0 0.7449277588013385 4.953965277657369E-5 80-81 1.9815861110629475E-4 0.0 0.0 0.8214417525147566 4.953965277657369E-5 82-83 1.9815861110629475E-4 0.0 0.0 0.918192694387405 4.953965277657369E-5 84-85 1.9815861110629475E-4 0.0 0.0 1.0692886353559548 4.953965277657369E-5 86-87 1.9815861110629475E-4 0.0 0.0 1.2944711270518705 4.953965277657369E-5 88-89 1.9815861110629475E-4 0.0 0.0 1.566542900100813 4.953965277657369E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11445 0.0 27.395931 1 GGTATCA 8340 0.0 23.75352 1 ATCAACG 13450 0.0 22.953672 3 TCAACGC 13495 0.0 22.877132 4 CAACGCA 13550 0.0 22.784273 5 TATCAAC 13635 0.0 22.74674 2 AACGCAG 13820 0.0 22.410093 6 GTACATG 8000 0.0 22.150156 1 CACGCGT 110 0.0028777074 21.59143 6 TACATGG 8095 0.0 21.239918 2 TACCGTC 160 4.9907518E-5 20.781755 7 GAGTACT 6850 0.0 20.698895 12-13 AGAGTAC 11415 0.0 20.203575 10-11 ACATGGG 8015 0.0 20.088953 3 ACGCAGA 15400 0.0 19.925806 7 AGTACTT 7285 0.0 19.82154 12-13 ACCGTCG 120 0.0047633736 19.792637 8 CGCAGAG 15465 0.0 19.719687 8 GTACTTT 7315 0.0 19.415575 14-15 GCAGAGT 16365 0.0 18.344927 9 >>END_MODULE