##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936983_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1046368 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.73656208905471 33.0 31.0 34.0 30.0 34.0 2 31.785505672956358 33.0 31.0 34.0 30.0 34.0 3 31.759848351631547 33.0 31.0 34.0 30.0 34.0 4 35.37596619927215 37.0 35.0 37.0 33.0 37.0 5 35.434980809810696 37.0 35.0 37.0 33.0 37.0 6 35.52348217835408 37.0 35.0 37.0 33.0 37.0 7 35.47563763417841 37.0 35.0 37.0 33.0 37.0 8 35.45545066362886 37.0 35.0 37.0 33.0 37.0 9 37.02082536927735 39.0 37.0 39.0 33.0 39.0 10-11 36.89001383834368 39.0 37.0 39.0 33.0 39.0 12-13 36.776425693446285 39.0 37.0 39.0 32.0 39.0 14-15 37.76375902168262 40.0 37.0 41.0 32.5 41.0 16-17 37.74953027997798 40.0 37.0 41.0 32.5 41.0 18-19 37.842862167038746 40.0 37.5 41.0 33.0 41.0 20-21 37.84315556286125 40.0 37.5 41.0 33.0 41.0 22-23 37.69532086225878 40.0 37.0 41.0 32.0 41.0 24-25 37.502082441359065 40.0 37.0 41.0 32.0 41.0 26-27 37.418542998256825 40.0 37.0 41.0 32.0 41.0 28-29 37.46214572693354 40.0 37.0 41.0 32.0 41.0 30-31 37.2467712124224 39.0 36.5 41.0 32.0 41.0 32-33 36.94157504816661 39.0 35.0 41.0 31.0 41.0 34-35 36.68257247928071 39.0 35.0 41.0 31.0 41.0 36-37 36.45899865057035 39.0 35.0 41.0 30.0 41.0 38-39 36.231396602342585 39.0 35.0 40.5 30.0 41.0 40-41 35.987492450074924 39.0 35.0 40.0 30.0 41.0 42-43 35.6737500573412 38.0 35.0 40.0 28.5 41.0 44-45 35.23202735557662 38.0 35.0 40.0 26.5 41.0 46-47 34.62820919599987 38.0 34.5 40.0 23.5 41.0 48-49 34.07272106945166 38.0 34.0 40.0 20.5 41.0 50-51 33.325789301660606 37.5 33.0 39.5 18.0 40.5 52-53 33.572822850087164 38.0 33.5 40.0 18.0 40.5 54-55 33.86634769029634 38.0 34.0 40.0 17.5 41.0 56-57 33.74004461145601 38.0 34.0 40.0 14.5 41.0 58-59 33.49578494372916 38.0 33.5 40.0 12.5 41.0 60-61 33.1728067945503 37.5 33.0 40.0 8.5 41.0 62-63 32.88237790146488 37.0 33.0 40.0 8.0 41.0 64-65 32.56321389797853 36.5 32.5 39.5 6.5 41.0 66-67 32.15763096730787 36.0 32.0 39.0 2.0 41.0 68-69 31.711430395424937 35.5 31.0 39.0 2.0 41.0 70-71 31.31709446389798 35.0 31.0 38.5 2.0 40.0 72-73 30.944228512339826 35.0 31.0 37.5 2.0 40.0 74-75 30.527100408269366 35.0 30.5 37.0 2.0 39.0 76-77 30.108462319184074 35.0 30.0 36.5 2.0 39.0 78-79 29.669951680479524 35.0 29.5 36.0 2.0 39.0 80-81 29.234825606287654 34.0 29.0 35.5 2.0 37.5 82-83 28.817008452093337 34.0 29.0 35.0 2.0 37.0 84-85 28.467530065904157 34.0 29.0 35.0 2.0 36.5 86-87 28.171131953576563 34.0 28.5 35.0 2.0 36.0 88-89 27.76741930181351 34.0 27.0 35.0 2.0 36.0 90-91 27.387523796599282 34.0 26.0 35.0 2.0 35.5 92-93 27.030176285972047 34.0 25.5 35.0 2.0 35.0 94-95 26.62003903024557 34.0 25.0 35.0 2.0 35.0 96-97 26.182217441664882 33.0 24.5 35.0 2.0 35.0 98-99 25.618991119759016 33.0 21.5 35.0 2.0 35.0 100-101 23.569018261261814 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6180850082802927 1101 2 0.48355278469070484 1101 3 0.5571540873217842 1101 4 0.3773775668632169 1101 5 0.4177534493684192 1101 6 0.5006426279114038 1101 7 0.4808507594648006 1101 8 0.4122658011855833 1101 9 0.4912091604452584 1101 10-11 0.5577886087511388 1101 12-13 0.6845281927882354 1101 14-15 0.9099363013647235 1101 16-17 0.8519375636897948 1101 18-19 0.943785891263957 1101 20-21 0.7565961965396681 1101 22-23 0.8554867685534262 1101 24-25 1.0099456608077304 1101 26-27 0.834729760443345 1101 28-29 0.9032866288977885 1101 30-31 0.6332832623850422 1101 32-33 0.652287827720194 1101 34-35 0.6290901616439157 1101 36-37 0.6888910948323712 1101 38-39 0.7539155216220053 1101 40-41 0.802632571248175 1101 42-43 0.8470446328627688 1101 44-45 0.7567075263953384 1101 46-47 0.8135939244449446 1101 48-49 0.8015185846919692 1101 50-51 0.6240018849956996 1101 52-53 0.8273289000549084 1101 54-55 0.7425151614251888 1101 56-57 0.7529331082281843 1101 58-59 0.9048327739946913 1101 60-61 1.0018501233744743 1101 62-63 0.9186116492451646 1101 64-65 1.0528994455507998 1101 66-67 0.9346904456322207 1101 68-69 1.0032803652802436 1101 70-71 0.9462478907301382 1101 72-73 0.7131434432278247 1101 74-75 0.7274695687848194 1101 76-77 0.845977519981215 1101 78-79 0.894411933727973 1101 80-81 1.0643468596092305 1101 82-83 0.8736787187399457 1101 84-85 0.9482953803097836 1101 86-87 0.9777902324230077 1101 88-89 0.9567899791072492 1101 90-91 0.8784876575754517 1101 92-93 0.9023136844496165 1101 94-95 1.1299221481684043 1101 96-97 1.099917244295824 1101 98-99 1.2741596317333155 1101 100-101 1.4320807077780628 1102 1 0.33100936561021754 1102 2 0.431426588575885 1102 3 0.4373369146152868 1102 4 0.347455870879287 1102 5 0.4162251716827896 1102 6 0.30937139804206737 1102 7 0.28773083919399767 1102 8 0.30604342667466256 1102 9 0.17608274903735577 1102 10-11 0.3478965484165144 1102 12-13 0.40729892384471356 1102 14-15 0.42374538188131083 1102 16-17 0.41318490916646766 1102 18-19 0.44466828968958794 1102 20-21 0.45857598324790416 1102 22-23 0.45602156466683397 1102 24-25 0.49981677807777203 1102 26-27 0.5081523848155314 1102 28-29 0.5515492743343344 1102 30-31 0.6373232927484409 1102 32-33 0.766145020682437 1102 34-35 0.6597901471899661 1102 36-37 0.46796854879452354 1102 38-39 0.5968096873815156 1102 40-41 0.7935796485687163 1102 42-43 0.90888479240202 1102 44-45 0.8270042568716462 1102 46-47 0.8595054278679086 1102 48-49 1.1495969675477653 1102 50-51 1.2226521085265247 1102 52-53 1.139490735767076 1102 54-55 1.2081314404126786 1102 56-57 1.318182074972981 1102 58-59 1.2450039370524095 1102 60-61 1.2789540573755218 1102 62-63 1.2788257900589741 1102 64-65 1.4253286894273316 1102 66-67 1.3308292197149783 1102 68-69 1.2702291366440441 1102 70-71 1.4305921413434142 1102 72-73 1.5993276916578516 1102 74-75 1.4361325380706838 1102 76-77 1.5124021082710897 1102 78-79 1.589492739389069 1102 80-81 1.6241574016929654 1102 82-83 1.5560334792946122 1102 84-85 1.4565730457241095 1102 86-87 1.4968118314745062 1102 88-89 1.313036864685543 1102 90-91 1.169186389772083 1102 92-93 1.325910918590452 1102 94-95 1.2883116548035787 1102 96-97 1.2367996791090192 1102 98-99 1.2585367322307128 1102 100-101 1.76348196254256 1103 1 0.3596641248276917 1103 2 0.39908172275427845 1103 3 0.44754302978401483 1103 4 0.4837234701943487 1103 5 0.32269178363223716 1103 6 0.13546981158901872 1103 7 0.2642509184930404 1103 8 0.3019103561602918 1103 9 0.3530406920225104 1103 10-11 0.5282584187631514 1103 12-13 0.39561279919335135 1103 14-15 0.6255126095411399 1103 16-17 0.7097426356754539 1103 18-19 0.7754612706478028 1103 20-21 0.6990161325900246 1103 22-23 0.7109487117431215 1103 24-25 0.8997678537055975 1103 26-27 0.8088115415595851 1103 28-29 0.8499444982577842 1103 30-31 0.9343457063445086 1103 32-33 0.9438254954188494 1103 34-35 0.9872505094758921 1103 36-37 1.0267330478344476 1103 38-39 1.0263444241580046 1103 40-41 1.0288671548141295 1103 42-43 1.1377025698943584 1103 44-45 1.0779107272615391 1103 46-47 1.0585227846861969 1103 48-49 1.089486576030005 1103 50-51 1.0825192004637287 1103 52-53 1.0888965124320364 1103 54-55 1.2202765852354318 1103 56-57 1.2973679658640833 1103 58-59 1.4720644984359765 1103 60-61 1.3516530298692615 1103 62-63 1.163751983204456 1103 64-65 1.2887687717109415 1103 66-67 1.365910733574843 1103 68-69 1.5312033550110833 1103 70-71 1.4841355220553147 1103 72-73 1.4764028453493303 1103 74-75 1.5869579715661182 1103 76-77 1.7243716731558578 1103 78-79 1.677329932679065 1103 80-81 1.7055182548282346 1103 82-83 1.7183216665143952 1103 84-85 1.7461349648062239 1103 86-87 1.6065015961747235 1103 88-89 1.536151836015673 1103 90-91 1.4522327725325646 1103 92-93 1.3442599193715417 1103 94-95 1.4550587601902656 1103 96-97 1.4110120014394063 1103 98-99 1.4933817468088542 1103 100-101 1.967659046102174 1104 1 0.6383308836047554 1104 2 0.5810268861540244 1104 3 0.42990515045557487 1104 4 0.36361025011264303 1104 5 0.38653848417155956 1104 6 0.41699356992035064 1104 7 0.46294534824293265 1104 8 0.45082826403033494 1104 9 0.5759226181644834 1104 10-11 0.6158019385601037 1104 12-13 0.6893301445590794 1104 14-15 0.8544700365570606 1104 16-17 0.8649721561745878 1104 18-19 0.8805082895358041 1104 20-21 0.7472368720030786 1104 22-23 0.9210167251773598 1104 24-25 0.7422420399244345 1104 26-27 0.943286886098214 1104 28-29 0.8067705884647722 1104 30-31 0.8653026545125329 1104 32-33 0.9309812998407239 1104 34-35 0.9539417178854421 1104 36-37 1.1072670294735687 1104 38-39 1.137299441793047 1104 40-41 1.0665196456275439 1104 42-43 1.1200864563790134 1104 44-45 1.2040663263276912 1104 46-47 1.3075695840233763 1104 48-49 1.3896404508652225 1104 50-51 1.3210105267872123 1104 52-53 1.3186450794371538 1104 54-55 1.2977297579812372 1104 56-57 1.4097036931214006 1104 58-59 1.38006750401086 1104 60-61 1.232414700840195 1104 62-63 1.426008265243972 1104 64-65 1.3258577172330135 1104 66-67 1.0851374226255928 1104 68-69 1.023525227855199 1104 70-71 0.9537736322906376 1104 72-73 0.9027468606497528 1104 74-75 0.9675474087116598 1104 76-77 1.077010494092299 1104 78-79 1.2402425959344896 1104 80-81 1.2476398348748532 1104 82-83 1.3856099206406753 1104 84-85 1.4265465847221286 1104 86-87 1.7140900103270447 1104 88-89 1.6292328625671253 1104 90-91 1.3312915052133683 1104 92-93 1.1652720675342714 1104 94-95 1.2464916415202119 1104 96-97 1.1483970566535966 1104 98-99 1.3123843426186497 1104 100-101 1.5424648335372453 1105 1 0.2798586943353989 1105 2 0.42738447392430245 1105 3 0.3367088066695416 1105 4 0.3232907145157 1105 5 0.2226837098322605 1105 6 0.17013635004253302 1105 7 0.330613925611587 1105 8 0.3067482859653978 1105 9 0.38005889254485936 1105 10-11 0.3461021517253968 1105 12-13 0.2994098438196957 1105 14-15 0.4330803909062695 1105 16-17 0.6471498209945636 1105 18-19 0.5655148026631451 1105 20-21 0.5958719771593977 1105 22-23 0.4518527596198467 1105 24-25 0.6294892350329917 1105 26-27 0.3224109050901447 1105 28-29 0.4316236853621973 1105 30-31 0.2252533375369481 1105 32-33 0.3956543925468523 1105 34-35 0.5327445579967076 1105 36-37 0.4841038375518778 1105 38-39 0.48303710539440203 1105 40-41 0.6377536870807532 1105 42-43 0.5363500723232946 1105 44-45 0.35875286995733546 1105 46-47 0.43887995508237765 1105 48-49 0.545937043267152 1105 50-51 0.39690253904352346 1105 52-53 0.4036263893927696 1105 54-55 0.5051075479861922 1105 56-57 0.5929035173454409 1105 58-59 0.6521297419971219 1105 60-61 0.572835233762639 1105 62-63 0.3733151724824566 1105 64-65 0.4691474420014501 1105 66-67 0.5430199684598236 1105 68-69 0.7934812007760961 1105 70-71 0.806828524429374 1105 72-73 0.8920212031747035 1105 74-75 0.9076783359575806 1105 76-77 0.7522376226755547 1105 78-79 0.924961996006683 1105 80-81 0.9718520511199102 1105 82-83 0.9839673754711455 1105 84-85 0.867237428585252 1105 86-87 1.0438148895503083 1105 88-89 1.0907620211596978 1105 90-91 0.8866018504229665 1105 92-93 0.7831176671805018 1105 94-95 0.8841785073442523 1105 96-97 0.7026771183598228 1105 98-99 0.7775536516178434 1105 100-101 1.036337600910123 1106 1 0.580169631603308 1106 2 0.551127604092148 1106 3 0.5132944381989759 1106 4 0.3831154409602249 1106 5 0.4415924586563449 1106 6 0.48850640500118914 1106 7 0.6043595313946284 1106 8 0.44132668869848146 1106 9 0.48714311297364077 1106 10-11 0.6295739544683769 1106 12-13 0.6787077593517026 1106 14-15 1.037428561426644 1106 16-17 0.885477295371949 1106 18-19 0.8868199469915155 1106 20-21 0.9137478993260473 1106 22-23 0.9315136003387678 1106 24-25 0.9320406040481828 1106 26-27 0.930808191815899 1106 28-29 0.8683949880309143 1106 30-31 0.758680579206505 1106 32-33 0.8520607895365018 1106 34-35 0.8200911936356619 1106 36-37 0.6685505375568326 1106 38-39 0.8678061304971436 1106 40-41 0.9853879892048312 1106 42-43 0.860058048755441 1106 44-45 0.8170377637534401 1106 46-47 0.43081786665340616 1106 48-49 0.7311264278292171 1106 50-51 0.7485116889172687 1106 52-53 0.8666684253387018 1106 54-55 0.7193675866057916 1106 56-57 0.8926544704572876 1106 58-59 1.0059060454085156 1106 60-61 0.903914106862608 1106 62-63 0.8525125780377323 1106 64-65 0.7868416540131378 1106 66-67 0.9713261525465668 1106 68-69 0.833239085610483 1106 70-71 0.7996225037332287 1106 72-73 0.6188400376550725 1106 74-75 0.693940157020112 1106 76-77 0.7186400586809079 1106 78-79 0.6924408294968067 1106 80-81 1.0088050639435977 1106 82-83 0.8310471397925774 1106 84-85 0.826633873601839 1106 86-87 0.9404218113703777 1106 88-89 1.055912786124793 1106 90-91 0.9087353355940273 1106 92-93 0.9261423738201024 1106 94-95 1.1171873700466328 1106 96-97 0.8584621359366906 1106 98-99 1.0794950290501326 1106 100-101 1.433814453908095 1107 1 0.22512776557459802 1107 2 0.2782567906950213 1107 3 0.3412449964126907 1107 4 0.26547389391078013 1107 5 0.21962276168665085 1107 6 0.23629149272048267 1107 7 0.44870363027819593 1107 8 0.3402521272949741 1107 9 0.4955482166419003 1107 10-11 0.5049298157123943 1107 12-13 0.5296268183628641 1107 14-15 0.38856791549363123 1107 16-17 0.6125257403480262 1107 18-19 0.6304004892562673 1107 20-21 0.5468380109687985 1107 22-23 0.6091691027311086 1107 24-25 0.6242973823575966 1107 26-27 0.6120482237138205 1107 28-29 0.6635435127518079 1107 30-31 0.7967058981857704 1107 32-33 0.6556403349910482 1107 34-35 0.7616292717855799 1107 36-37 0.8471918852442286 1107 38-39 0.7887760268288773 1107 40-41 0.8570716016450248 1107 42-43 0.9247430567299233 1107 44-45 0.7602813841681169 1107 46-47 0.8610640787746178 1107 48-49 0.8957376718483587 1107 50-51 0.7117842168279509 1107 52-53 0.872663241925629 1107 54-55 1.0577886392373657 1107 56-57 0.933182779867181 1107 58-59 1.0087150565727896 1107 60-61 1.0106425757621267 1107 62-63 1.1406804408766504 1107 64-65 1.1351450830402499 1107 66-67 1.1509513519086099 1107 68-69 1.148635816073 1107 70-71 1.0893194734301979 1107 72-73 1.1881323024078618 1107 74-75 1.1357483707362235 1107 76-77 1.0906416535772365 1107 78-79 1.1081984052543845 1107 80-81 1.1544599682498173 1107 82-83 1.1974426740828505 1107 84-85 1.2006041686576587 1107 86-87 1.1221781909557045 1107 88-89 1.2793577622013501 1107 90-91 1.2816490118300727 1107 92-93 1.5132615923209016 1107 94-95 1.5122750893448753 1107 96-97 1.3768941534805492 1107 98-99 1.5570807547279664 1107 100-101 1.7500235846895897 1108 1 0.003180138901363705 1108 2 0.13419421370561224 1108 3 -0.07518556940812005 1108 4 0.007043553925562662 1108 5 -0.10700736067518335 1108 6 0.03594709344315561 1108 7 0.05794389881724982 1108 8 -0.14114466583962582 1108 9 -0.21306227855396287 1108 10-11 -0.11850858249734841 1108 12-13 -0.2876080301219801 1108 14-15 -0.05959896674337273 1108 16-17 -0.2367354179832617 1108 18-19 0.00997879261505119 1108 20-21 0.08752434586244107 1108 22-23 -0.01976248961511118 1108 24-25 0.2352750451645278 1108 26-27 0.16913843476744717 1108 28-29 -0.02074731216401915 1108 30-31 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-1.8243930366525767 2316 88-89 -2.249215878808407 2316 90-91 -2.2019048509028742 2316 92-93 -2.2838755585747705 2316 94-95 -2.498514234902707 2316 96-97 -2.469082919205423 2316 98-99 -2.5102960585496135 2316 100-101 -2.3757354429806377 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2210.0 3 600.0 4 631.0 5 622.0 6 850.0 7 1005.0 8 1357.0 9 1676.0 10 2244.0 11 3317.0 12 4832.0 13 6590.0 14 7623.0 15 10332.0 16 13849.0 17 18793.0 18 15242.0 19 8349.0 20 7358.0 21 7352.0 22 7610.0 23 8278.0 24 9501.0 25 10680.0 26 12518.0 27 14460.0 28 17280.0 29 20560.0 30 25072.0 31 31197.0 32 39062.0 33 50759.0 34 68166.0 35 98103.0 36 135927.0 37 185275.0 38 165986.0 39 31092.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.71391687266016 19.883719262344073 15.349248621795944 19.053115243199827 2 14.886615295575103 22.688229132287734 40.20806727030091 22.217088301836256 3 19.32144421428722 29.22129713043029 31.306387723757773 20.150870931524718 4 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19.92361203098557 24-25 20.458054394855914 26.4391838811214 33.63576073760729 19.467000986415393 26-27 20.19500072194157 25.9094790450482 34.540553514038656 19.35496671897157 28-29 19.280377254917713 26.98059309966251 34.17533823167974 19.563691413740045 30-31 19.593279555884262 26.61367007903114 33.80062312742655 19.99242723765805 32-33 19.629953133388884 26.79380842489844 33.615498730446944 19.960739711265724 34-35 20.400939675473495 26.34112667356986 33.45562117730972 19.80231247364692 36-37 20.41746646810077 25.96467407543751 33.87597918404716 19.741880272414562 38-39 20.113145286943734 26.662194911474067 33.57837149385149 19.646288307730703 40-41 19.682946352491587 26.982027601732494 33.570695609180426 19.764330436595486 42-43 19.478669586588758 27.493425393759146 32.215905287417876 20.811999732234217 44-45 19.918925211639237 27.051622906137695 32.02611910506973 21.003332777153336 46-47 20.72498338838669 27.523889651084417 31.37998288645305 20.371144074075843 48-49 20.600686222625075 27.738281173433997 30.668041597884248 20.992991006056677 50-51 20.20340201509192 28.201201369142108 31.00210170291949 20.593294912846485 52-53 20.458426394774282 29.06088895347948 29.614668267833377 20.866016383912864 54-55 19.83250960222823 28.130345527230933 30.307997984416517 21.729146886124315 56-57 20.73273873413001 28.50485631847313 29.29434572971474 21.468059217682114 58-59 21.37254133201497 27.815257918084725 29.633015503868553 21.179185246031754 60-61 20.892924847874596 28.123369914192043 30.585728827351833 20.39797641058153 62-63 20.82065234282545 28.16124243025555 30.50767792635024 20.51042730056876 64-65 20.449709460057033 28.90622647056714 30.229109449061692 20.414954620314134 66-67 20.664478006451223 28.44802442016921 29.37028363009028 21.51721394328928 68-69 20.423292110474584 28.628897692533506 29.890015003169886 21.05779519382202 70-71 21.09469586709627 28.642919928246442 29.38029147852388 20.882092726133408 72-73 20.72971566248476 28.151999720876926 29.929258550653433 21.189026065984887 74-75 20.214399271920072 29.82938494645037 29.126734280461818 20.829481501167738 76-77 20.469208940154203 28.681225905641906 29.436325878105496 21.4132392760984 78-79 20.343881190769906 28.70293867324593 29.66802459572922 21.285155540254948 80-81 20.560915201587864 29.412051531000778 28.920767907328717 21.106265360082645 82-83 20.378225994621907 28.95931431238071 29.409767778113537 21.25269191488385 84-85 20.23066330043608 28.95426899242598 28.91496442506312 21.900103282074824 86-87 20.29340940562624 29.227042514287465 28.920110703930103 21.559437376156193 88-89 20.451666007441037 28.570424188973124 28.60419057057054 22.373719233015297 90-91 20.08583748447208 28.154618402845184 29.320921834910113 22.438622277772623 92-93 20.439759079356044 28.97895650469001 29.193432306224583 21.38785210972936 94-95 20.005480704667495 28.585174741699078 29.223299020742505 22.186045532890926 96-97 19.396376903092012 28.582490596936687 29.519735910710114 22.501396589261187 98-99 19.836548945610566 28.949839893175998 28.484183321237012 22.729427839976424 100-101 19.657922157699694 28.380663143602654 29.323671220241255 22.637743478456397 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1739.0 1 1214.5 2 828.0 3 973.0 4 888.5 5 946.0 6 1505.0 7 3137.0 8 5136.5 9 5718.0 10 4638.0 11 3674.5 12 3579.5 13 3610.5 14 3644.5 15 3629.5 16 3639.5 17 3675.0 18 3729.0 19 3928.0 20 4247.5 21 4596.0 22 5061.5 23 5824.5 24 6436.5 25 7196.0 26 8604.0 27 10009.5 28 11034.0 29 12502.0 30 14666.5 31 16614.0 32 19288.0 33 22650.5 34 25898.5 35 27952.5 36 31055.5 37 34690.5 38 34732.0 39 33644.5 40 32627.0 41 31800.5 42 30501.0 43 30289.5 44 32121.5 45 32807.0 46 32451.5 47 37773.5 48 56868.0 49 55923.0 50 35378.5 51 26676.0 52 22958.0 53 22144.0 54 21731.0 55 20159.0 56 18559.5 57 16948.0 58 15945.5 59 15182.0 60 14029.5 61 12507.0 62 10366.0 63 7541.5 64 4999.0 65 3584.0 66 2497.0 67 1709.5 68 1201.0 69 859.0 70 671.5 71 506.0 72 393.5 73 318.5 74 216.0 75 170.0 76 133.0 77 63.5 78 30.0 79 16.0 80 8.0 81 6.5 82 4.5 83 5.0 84 3.5 85 2.0 86 2.0 87 2.0 88 2.5 89 2.0 90 1.5 91 1.0 92 0.5 93 2.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05953928254686687 2 0.09872243799504572 3 0.06747148230832747 4 0.13284045383650875 5 0.10675020642833115 6 0.10120722346249121 7 0.11162420869139729 8 0.09661992721489954 9 0.11793174103183583 10-11 0.11114636533227316 12-13 0.09270161167008166 14-15 0.09791010428453469 16-17 0.11267546408147038 18-19 0.08926113948438791 20-21 0.07344452429737913 22-23 0.07497362304657636 24-25 0.06331424508394752 26-27 0.05514312364292486 28-29 0.06775818832380195 30-31 0.04917008165387321 32-33 0.050412474387595954 34-35 0.045395119116792566 36-37 0.05117702376219456 38-39 0.052037141808618 40-41 0.06775818832380195 42-43 0.06479555949723233 44-45 0.05194157313679318 46-47 0.03946986146365332 48-49 0.04754541423285116 50-51 0.029769641273433435 52-53 0.044343863726719474 54-55 0.04940900333343527 56-57 0.05404408391693936 58-59 0.06011269457781584 60-61 0.05758012477445793 62-63 0.06455663781767026 64-65 0.044965060093580846 66-67 0.06450885348175785 68-69 0.05628994770482278 70-71 0.027475993149637605 72-73 0.022410853542921803 74-75 0.0314420930303679 76-77 0.04587296247591669 78-79 0.0628841860607358 80-81 0.041524587907887094 82-83 0.061546224655188234 84-85 0.06575124621548059 86-87 0.06651579559007921 88-89 0.0907424538976727 90-91 0.06412657879445854 92-93 0.07602487843664944 94-95 0.08429156854949692 96-97 0.09212819963913269 98-99 0.12275795895898957 100-101 0.1199386831401572 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1046368.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.72978028437093 #Duplication Level Percentage of deduplicated Percentage of total 1 72.79819120154123 30.3785252393994 2 13.856862732577477 11.564876745222918 3 5.1476159622882784 6.444266492838315 4 2.52326928232767 4.21181890999344 5 1.369323759644407 2.8570789814064925 6 0.8916452517830459 2.232489626910546 7 0.5754933125612218 1.6810646641813212 8 0.3934376470241926 1.3134453252735552 9 0.2985253528142038 1.1211657644025934 >10 1.8455560043040387 14.867795405464584 >50 0.18161757407368628 5.220720652504212 >100 0.10624697413265426 7.8715308492815454 >500 0.005300825157410968 1.546684735819074 >1k 0.005761766475446705 4.51925853352433 >5k 6.914119770536046E-4 1.8866630060044007 >10k+ 4.6094131803573645E-4 2.2826150677732806 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13672 1.3066148811890272 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10062 0.9616119759014037 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7480 0.7148536652497017 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7051 0.6738547050368513 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5086 0.48606226490106735 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3947 0.37720954769258996 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3224 0.30811339796324044 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2897 0.2768624422765222 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2631 0.2514411755711184 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2447 0.2338565399553503 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2254 0.21541178629315882 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2197 0.20996437199914372 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2153 0.20575935043885135 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2084 0.1991651120829383 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2077 0.19849613138016453 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2048 0.19572463989724456 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1905 0.1820583198262944 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1902 0.1817716138108199 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1694 0.161893330071256 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1628 0.15558579773081746 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1588 0.1517630508578244 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1301 0.12433484204409921 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1260 0.12041652649928132 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1231 0.11764503501636135 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1163 0.11114636533227316 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1108 0.1058900883819077 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1098 0.10493440166365944 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1073 0.10254518486803878 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1070 0.1022584788525643 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 9.556867182482645E-5 0.0 16-17 0.0 0.0 0.0 9.556867182482645E-5 0.0 18-19 0.0 0.0 0.0 9.556867182482645E-5 0.0 20-21 0.0 0.0 0.0 1.4335300773723968E-4 0.0 22-23 0.0 0.0 0.0 8.123337105110247E-4 0.0 24-25 0.0 0.0 0.0 0.0018635891005841158 0.0 26-27 0.0 0.0 0.0 0.0022458637878834216 0.0 28-29 0.0 0.0 0.0 0.00458729624759167 0.0 30-31 0.0 0.0 0.0 0.008840102143796446 0.0 32-33 0.0 0.0 0.0 0.013857457414599834 0.0 34-35 0.0 0.0 0.0 0.01973493073182666 0.0 36-37 0.0 0.0 0.0 0.02589911006452797 0.0 38-39 0.0 0.0 0.0 0.029865209945258264 0.0 40-41 0.0 0.0 0.0 0.03373574115416374 0.0 42-43 0.0 0.0 0.0 0.038514174745405055 0.0 44-45 0.0 0.0 0.0 0.04367488302394569 0.0 46-47 0.0 0.0 0.0 0.052706122511391784 0.0 48-49 0.0 0.0 0.0 0.06374430410715924 0.0 50-51 0.0 0.0 0.0 0.07430464234380256 0.0 52-53 0.0 0.0 0.0 0.09475633811431541 0.0 54-55 0.0 0.0 0.0 0.13503853328847978 0.0 56-57 0.0 0.0 0.0 0.21005994067096853 0.0 58-59 0.0 0.0 0.0 0.2869449371540414 0.0 60-61 0.0 0.0 0.0 0.3547509098137558 0.0 62-63 0.0 0.0 0.0 0.4351241628184348 0.0 64-65 0.0 0.0 0.0 0.5302149912841372 0.0 66-67 0.0 0.0 0.0 0.6890023395210862 0.0 68-69 0.0 0.0 0.0 0.8795662711397902 0.0 70-71 0.0 0.0 0.0 1.0346742255114836 0.0 72-73 0.0 0.0 0.0 1.19618528089544 0.0 74-75 0.0 0.0 0.0 1.3739907948255299 0.0 76-77 0.0 0.0 0.0 1.561448744609927 0.0 78-79 0.0 0.0 0.0 1.7235332120248326 0.0 80-81 0.0 0.0 0.0 1.8997140585339 0.0 82-83 0.0 0.0 0.0 2.0774240037921645 0.0 84-85 0.0 0.0 0.0 2.4047944891281077 0.0 86-87 0.0 0.0 0.0 2.824054252423622 0.0 88-89 0.0 0.0 0.0 3.284886387962935 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTATC 55 0.0025762268 34.55445 1 CGTCGAG 30 0.003953127 31.664307 38-39 GTACATG 10325 0.0 25.03315 1 GAGTACT 8450 0.0 24.708508 12-13 TACATGG 10535 0.0 24.263554 2 CATATAG 260 2.3646862E-11 23.753912 3 AGTACTT 8775 0.0 23.387346 12-13 ACATGGG 10585 0.0 23.159224 3 GTACTTT 9280 0.0 22.396206 14-15 CTTTGCG 255 4.8930815E-10 22.358763 2 TAAGGTG 340 0.0 22.353413 5 CATGGGA 7740 0.0 22.219421 4 CATGGGG 2265 0.0 21.813747 4 CGCGGAA 55 0.0028773926 21.591883 82-83 ATGGGAT 2715 0.0 20.819962 5 GTATGGT 160 4.9787377E-5 20.78666 1 CATGGGT 625 0.0 20.523378 4 TACTTTT 10095 0.0 20.235153 14-15 ACTTTTT 10485 0.0 20.139935 16-17 CAGTCGA 120 0.004761314 19.793034 9 >>END_MODULE