##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936983_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1046368 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.032693086944555 31.0 31.0 34.0 30.0 34.0 2 31.980018502094865 31.0 31.0 34.0 30.0 34.0 3 32.13691072356953 33.0 31.0 34.0 30.0 34.0 4 35.92092934799229 37.0 35.0 37.0 35.0 37.0 5 35.86579004709624 37.0 35.0 37.0 35.0 37.0 6 35.94879334994954 37.0 35.0 37.0 35.0 37.0 7 35.880633773204075 37.0 35.0 37.0 35.0 37.0 8 35.87637141044068 37.0 35.0 37.0 35.0 37.0 9 37.50141632771644 39.0 37.0 39.0 35.0 39.0 10-11 37.43077865531056 39.0 37.0 39.0 34.0 39.0 12-13 37.338525738554694 39.0 37.0 39.0 34.0 39.0 14-15 38.354772412764916 40.0 38.0 41.0 33.5 41.0 16-17 38.321740057035385 40.0 38.0 41.0 33.5 41.0 18-19 38.508464995106884 40.0 38.0 41.0 34.0 41.0 20-21 38.63024958714334 40.0 38.0 41.0 34.0 41.0 22-23 38.5449870408881 40.0 38.0 41.0 34.0 41.0 24-25 38.41711233523961 40.0 38.0 41.0 34.0 41.0 26-27 38.203499151350194 40.0 37.5 41.0 34.0 41.0 28-29 38.0197387534787 40.0 37.0 41.0 33.0 41.0 30-31 37.75877177054345 39.5 37.0 41.0 33.0 41.0 32-33 37.55582691748983 40.0 36.0 41.0 33.0 41.0 34-35 37.387798078687425 40.0 35.0 41.0 33.0 41.0 36-37 37.13040631976513 39.5 35.0 41.0 32.5 41.0 38-39 36.906543395822496 39.0 35.0 41.0 31.5 41.0 40-41 36.66792466895012 39.0 35.0 41.0 31.0 41.0 42-43 36.320337586776354 39.0 35.0 41.0 30.5 41.0 44-45 35.77821665035628 39.0 35.0 41.0 29.5 41.0 46-47 35.19830881678339 39.0 35.0 41.0 26.0 41.0 48-49 34.62012026361663 39.0 35.0 40.0 23.0 41.0 50-51 34.28528060873421 38.0 34.0 40.0 21.0 41.0 52-53 34.02062085231964 38.0 34.0 40.0 19.5 41.0 54-55 33.79387892213829 38.0 34.0 40.0 18.0 41.0 56-57 33.515916484449065 38.0 33.5 40.0 15.5 41.0 58-59 33.20958926496223 37.5 33.0 40.0 12.5 41.0 60-61 32.84135600477079 37.0 33.0 40.0 8.0 41.0 62-63 32.500983879476436 36.5 32.0 39.5 7.5 41.0 64-65 32.098974739288664 36.0 32.0 39.0 4.0 40.5 66-67 31.705709654729503 35.5 31.0 39.0 2.0 40.0 68-69 31.265574826447292 35.0 31.0 38.0 2.0 40.0 70-71 30.765115141135816 35.0 30.5 37.5 2.0 40.0 72-73 30.432136208293834 35.0 30.0 37.0 2.0 39.0 74-75 30.015678040612862 34.5 30.0 36.5 2.0 39.0 76-77 28.620517351448058 33.0 28.0 35.0 2.0 37.5 78-79 29.301800131502493 34.0 29.0 36.0 2.0 37.5 80-81 29.127129270008258 34.0 29.0 35.0 2.0 37.0 82-83 28.801371983852718 34.0 29.0 35.0 2.0 37.0 84-85 28.42324497691061 34.0 29.0 35.0 2.0 36.0 86-87 28.098797937245788 34.0 29.0 35.0 2.0 36.0 88-89 27.716555743294904 34.0 27.5 35.0 2.0 35.5 90-91 27.269907910027833 33.0 26.5 35.0 2.0 35.0 92-93 26.847298464784856 33.0 25.5 35.0 2.0 35.0 94-95 26.39459683400104 33.0 25.0 35.0 2.0 35.0 96-97 25.78353026850974 33.0 23.5 35.0 2.0 35.0 98-99 25.124805039909475 32.0 19.0 34.0 2.0 35.0 100-101 22.35864342181718 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.40555697742506425 1101 2 0.2719831501822654 1101 3 0.2287127940765572 1101 4 0.20897299394877678 1101 5 0.37230931949476087 1101 6 0.2472925104832413 1101 7 0.24954167738854238 1101 8 0.2974595291889912 1101 9 0.488000732879982 1101 10-11 0.4535533745061926 1101 12-13 0.4224951955960634 1101 14-15 0.5366957172508862 1101 16-17 0.49168027278634696 1101 18-19 0.4629574041246656 1101 20-21 0.4872856332434523 1101 22-23 0.5065264670542788 1101 24-25 0.5032168319772694 1101 26-27 0.39994220318668994 1101 28-29 0.5382134268493246 1101 30-31 0.582387851792852 1101 32-33 0.6791156638681244 1101 34-35 0.6188305132423011 1101 36-37 0.701081394145298 1101 38-39 0.738854922179982 1101 40-41 0.6688907981018986 1101 42-43 0.7375876387143094 1101 44-45 0.8027543563887392 1101 46-47 0.9038970841443756 1101 48-49 0.9846032241598337 1101 50-51 0.8482612874233197 1101 52-53 0.7953317268193203 1101 54-55 0.8114499918456488 1101 56-57 0.8612174661063747 1101 58-59 0.8308688137421285 1101 60-61 0.7953703042747051 1101 62-63 1.2040029918433888 1101 64-65 0.914411485039011 1101 66-67 0.7956937551072798 1101 68-69 0.8896869604390751 1101 70-71 0.731125112882328 1101 72-73 1.0120076716584983 1101 74-75 0.5996681632314491 1101 76-77 0.38094525649246336 1101 78-79 0.5506814310494619 1101 80-81 1.0679726066203479 1101 82-83 1.0787055447347207 1101 84-85 1.0571925066820036 1101 86-87 0.8918006530537674 1101 88-89 1.0740673547682 1101 90-91 1.2647642033461288 1101 92-93 1.1420705972627623 1101 94-95 1.0524560847970506 1101 96-97 0.9025958279349489 1101 98-99 1.319594159590583 1101 100-101 1.4721986566834993 1102 1 0.2437192437234046 1102 2 0.16018337073133182 1102 3 0.2050517806222487 1102 4 0.2000236747499855 1102 5 0.20587159125123833 1102 6 0.28580470385484347 1102 7 0.27863252399576055 1102 8 0.32103664909308804 1102 9 0.2632903425121782 1102 10-11 0.3165122494835728 1102 12-13 0.36459752753352603 1102 14-15 0.553442082790248 1102 16-17 0.4215872115915644 1102 18-19 0.3538821435240038 1102 20-21 0.3913771115792102 1102 22-23 0.32897183264316965 1102 24-25 0.39033401711618154 1102 26-27 0.4948485506939946 1102 28-29 0.4349116597277032 1102 30-31 0.49755782720092867 1102 32-33 0.6012543631542187 1102 34-35 0.6223348378032014 1102 36-37 0.6276987960934548 1102 38-39 0.707753662664885 1102 40-41 0.733581822199568 1102 42-43 0.9147713221263345 1102 44-45 0.9180902995190152 1102 46-47 1.0679226201281367 1102 48-49 0.7997311517162373 1102 50-51 0.9419107478260713 1102 52-53 0.9350518862372823 1102 54-55 0.802813504620211 1102 56-57 0.9426467696180723 1102 58-59 0.8720832891193737 1102 60-61 0.9381657182287313 1102 62-63 0.8681735727000941 1102 64-65 0.88978670652304 1102 66-67 0.9466667686747883 1102 68-69 0.8014127518433192 1102 70-71 0.774783619637784 1102 72-73 0.9331275191541728 1102 74-75 0.5710296342593679 1102 76-77 0.5578757431053738 1102 78-79 0.6723584125797402 1102 80-81 0.7094777782028743 1102 82-83 0.7537064796929549 1102 84-85 0.7056861120730247 1102 86-87 0.8009783042162972 1102 88-89 0.7062770199116422 1102 90-91 0.8993997060858092 1102 92-93 0.6925204385220134 1102 94-95 0.7980807329242552 1102 96-97 0.826351752285202 1102 98-99 1.2570227110926346 1102 100-101 1.4099481262842168 1103 1 0.06092248859933136 1103 2 -0.17564749828074255 1103 3 0.05701305292038228 1103 4 0.07943265322231952 1103 5 0.08695514331177634 1103 6 0.15185865100329465 1103 7 0.27216090627077705 1103 8 0.28011868430918696 1103 9 0.2675943649833883 1103 10-11 0.24361918512725111 1103 12-13 0.30778339197900806 1103 14-15 0.3970300149043666 1103 16-17 0.3819254131203067 1103 18-19 0.4703589842562863 1103 20-21 0.4467334330708965 1103 22-23 0.3397498340542242 1103 24-25 0.503935842618624 1103 26-27 0.427780603430314 1103 28-29 0.401093868068088 1103 30-31 0.4881889988263026 1103 32-33 0.5000904731188243 1103 34-35 0.5582136867015066 1103 36-37 0.39304031749031054 1103 38-39 0.44460193194160524 1103 40-41 0.2865632337715951 1103 42-43 0.29766309253691503 1103 44-45 0.3147998993686585 1103 46-47 0.5232866864046102 1103 48-49 0.3928574663226243 1103 50-51 0.25078882130141267 1103 52-53 0.2391135536138833 1103 54-55 0.3559271442251486 1103 56-57 0.16990142286422838 1103 58-59 0.26521045376311037 1103 60-61 0.2746374209574469 1103 62-63 0.2755487801328158 1103 64-65 0.27307091084557555 1103 66-67 0.42987805868203566 1103 68-69 0.36089269898988263 1103 70-71 0.3797995956176159 1103 72-73 0.32666364621398003 1103 74-75 0.39307525859763714 1103 76-77 0.23399639929643357 1103 78-79 0.35429001857420417 1103 80-81 0.31948252896719964 1103 82-83 0.39144133793034186 1103 84-85 0.4688387872822517 1103 86-87 0.2675217784323003 1103 88-89 0.42680551646946085 1103 90-91 0.6370932902872077 1103 92-93 0.7413701963085906 1103 94-95 0.9172144126320703 1103 96-97 1.1040372327971504 1103 98-99 1.1005412234894827 1103 100-101 1.1316836758853164 1104 1 0.32180563173415777 1104 2 0.19677029555536762 1104 3 0.29012491121926587 1104 4 0.2032195640193919 1104 5 0.1765924396949572 1104 6 0.18912929374775445 1104 7 0.24756175210920617 1104 8 0.3057559731216557 1104 9 0.19809612375571817 1104 10-11 0.31357924012851157 1104 12-13 0.39641993412945453 1104 14-15 0.48704111546904727 1104 16-17 0.4991300581340923 1104 18-19 0.49890342862712345 1104 20-21 0.42138146567797463 1104 22-23 0.4676277713743673 1104 24-25 0.5283608702754066 1104 26-27 0.38684431385792806 1104 28-29 0.4613553606539895 1104 30-31 0.4234920120665393 1104 32-33 0.581002517468086 1104 34-35 0.5880479920834318 1104 36-37 0.7626768189481439 1104 38-39 0.6687311338081656 1104 40-41 0.8305590395940499 1104 42-43 0.823498166346674 1104 44-45 0.8797699051318659 1104 46-47 0.8427984129730035 1104 48-49 0.7937728607144265 1104 50-51 0.6932225041810867 1104 52-53 0.7196676922886169 1104 54-55 0.673571742565457 1104 56-57 0.5854751460390446 1104 58-59 0.750190150044844 1104 60-61 0.7489580200283328 1104 62-63 0.8072133064945248 1104 64-65 0.7853993512872108 1104 66-67 0.7317391303360381 1104 68-69 0.6137679155833844 1104 70-71 0.8196313174921634 1104 72-73 0.7475169985750476 1104 74-75 0.8682007195833634 1104 76-77 0.6447087754323952 1104 78-79 0.5821151501390815 1104 80-81 0.7122286458988256 1104 82-83 0.6042546828342523 1104 84-85 0.80420312333505 1104 86-87 0.6821969520619788 1104 88-89 0.8122402128789581 1104 90-91 1.0981253872908106 1104 92-93 1.1967332453393986 1104 94-95 1.0827296993304145 1104 96-97 1.1618571689570736 1104 98-99 0.9998185038605989 1104 100-101 1.128858851880839 1105 1 0.16096231329808575 1105 2 -0.05235059381194063 1105 3 0.10111958716463931 1105 4 0.13192505341790195 1105 5 0.18098406928773159 1105 6 0.1708066802250059 1105 7 0.2832265480789147 1105 8 0.17427388814542155 1105 9 0.17024338782679393 1105 10-11 0.214690487667049 1105 12-13 0.3740352709518433 1105 14-15 0.43906230828993387 1105 16-17 0.3587091559343989 1105 18-19 0.3183125481132407 1105 20-21 0.36403640286852834 1105 22-23 0.4144860067645979 1105 24-25 0.39537951969928287 1105 26-27 0.3609658263805855 1105 28-29 0.25864754296246417 1105 30-31 0.5299514095722557 1105 32-33 0.5192875201038092 1105 34-35 0.3304291101995247 1105 36-37 0.35968871983043726 1105 38-39 0.47214039361963245 1105 40-41 -0.004156159949531002 1105 42-43 0.11435199995219847 1105 44-45 0.23795682773228322 1105 46-47 0.20238443472157996 1105 48-49 0.3152546410369297 1105 50-51 0.15985969021465252 1105 52-53 0.2201002082575414 1105 54-55 0.1270321934345091 1105 56-57 0.24473103393385998 1105 58-59 0.1885379298115879 1105 60-61 0.2884531441839542 1105 62-63 0.3168929861834542 1105 64-65 0.3613624494124821 1105 66-67 0.2977829801620544 1105 68-69 0.2731078140506078 1105 70-71 0.37713932388435367 1105 72-73 0.384566399561205 1105 74-75 0.2594924236778198 1105 76-77 0.3578380584713976 1105 78-79 0.3860137022953367 1105 80-81 0.6047121922897425 1105 82-83 0.5816641475945872 1105 84-85 0.5187947442498029 1105 86-87 0.5712531440566018 1105 88-89 0.6775786508546879 1105 90-91 0.5964544077736953 1105 92-93 0.7412976228256731 1105 94-95 0.9959140046783759 1105 96-97 1.2492300106470005 1105 98-99 1.096075232784333 1105 100-101 0.9650086935070625 1106 1 0.34817823645499146 1106 2 0.2559872781492416 1106 3 0.2992391098660292 1106 4 0.2643496709353599 1106 5 0.19623088812220857 1106 6 0.27666299551935936 1106 7 0.22708553703766654 1106 8 0.2749621091683494 1106 9 0.3839553252432424 1106 10-11 0.460880515889059 1106 12-13 0.3731866300645805 1106 14-15 0.5968401960950516 1106 16-17 0.6666647929101899 1106 18-19 0.6023794887479852 1106 20-21 0.4857376456273599 1106 22-23 0.49850789120288397 1106 24-25 0.4824944377564222 1106 26-27 0.6040598502455126 1106 28-29 0.6913197219989584 1106 30-31 0.7993537960652972 1106 32-33 0.6532539507618296 1106 34-35 0.5683248372877117 1106 36-37 0.574817204258828 1106 38-39 0.6749952730571707 1106 40-41 0.6247421912907569 1106 42-43 0.7258126129145168 1106 44-45 0.707068081878937 1106 46-47 0.39712721830330366 1106 48-49 0.3065330487212421 1106 50-51 0.17962351652548847 1106 52-53 0.22487765045192987 1106 54-55 0.3030702188838319 1106 56-57 0.3042205620816034 1106 58-59 0.26821659495988115 1106 60-61 0.15527742501773645 1106 62-63 0.3547976254863201 1106 64-65 0.444091567598349 1106 66-67 0.4007195549008813 1106 68-69 0.2532989315433021 1106 70-71 0.2443552470412591 1106 72-73 0.20958249105994398 1106 74-75 0.49183534589398903 1106 76-77 0.7222352461725414 1106 78-79 0.5381427313590592 1106 80-81 0.49212121343149207 1106 82-83 0.5825548739400865 1106 84-85 0.5960057161763288 1106 86-87 0.579550911051701 1106 88-89 0.7706617820127839 1106 90-91 1.1055072374018557 1106 92-93 0.9171479966435676 1106 94-95 0.9320639279343084 1106 96-97 1.0989735369132738 1106 98-99 1.0704610326556008 1106 100-101 1.0223018558579078 1107 1 0.19165909769900935 1107 2 0.1185769910679042 1107 3 0.05157962023285023 1107 4 0.1370590010788959 1107 5 0.20693264250817833 1107 6 0.20575191098985357 1107 7 0.2171453456501098 1107 8 0.2050259050216212 1107 9 0.31402510195776046 1107 10-11 0.2247062734648182 1107 12-13 0.2907593972097189 1107 14-15 0.43160993293715677 1107 16-17 0.36237053453698564 1107 18-19 0.34527223196967327 1107 20-21 0.19601475886499742 1107 22-23 0.22875210970368443 1107 24-25 0.22947809006902276 1107 26-27 0.3582344913938442 1107 28-29 0.38135361354601116 1107 30-31 0.6450754866554078 1107 32-33 0.7301758008415717 1107 34-35 0.7287933380851612 1107 36-37 0.6963582728393689 1107 38-39 0.7289366606169807 1107 40-41 0.8028562263784735 1107 42-43 0.7621941918618873 1107 44-45 0.7467819973494301 1107 46-47 0.8411493474899103 1107 48-49 0.9060296595665349 1107 50-51 0.9066201273707861 1107 52-53 1.0033176424774481 1107 54-55 0.7973246048487468 1107 56-57 0.7359829225281729 1107 58-59 0.8885694657732728 1107 60-61 0.9493364361820156 1107 62-63 0.6277132953427795 1107 64-65 0.6849926840257865 1107 66-67 0.6615788052996052 1107 68-69 0.8458507656740828 1107 70-71 0.7141808052549727 1107 72-73 0.7625779057329893 1107 74-75 0.7504298435016423 1107 76-77 0.6516730452156061 1107 78-79 0.8103915351868096 1107 80-81 0.9835735659035834 1107 82-83 0.9396431913244534 1107 84-85 0.8147726619498528 1107 86-87 0.7258537419926903 1107 88-89 0.9287309606411718 1107 90-91 1.0421310412296094 1107 92-93 1.1185385468827995 1107 94-95 1.1269134095930013 1107 96-97 1.178955594329544 1107 98-99 0.993854174601374 1107 100-101 0.8294788893507281 1108 1 -0.010581807285802824 1108 2 -0.08925591112475573 1108 3 -0.01414366957580171 1108 4 0.026550973650138587 1108 5 0.09288566870510806 1108 6 0.04192615341409578 1108 7 0.02879332955419045 1108 8 0.0957009481356863 1108 9 0.14912149269439112 1108 10-11 0.027456848319054927 1108 12-13 -0.03003877762808571 1108 14-15 -0.010251516927340276 1108 16-17 0.11743643746874 1108 18-19 -0.11847345662357611 1108 20-21 0.020373693742222088 1108 22-23 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-1.4235836190076263 2316 100-101 -1.610160261957926 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 16.0 5 33.0 6 93.0 7 193.0 8 443.0 9 872.0 10 1620.0 11 2689.0 12 4529.0 13 6102.0 14 7600.0 15 10838.0 16 14966.0 17 21039.0 18 17412.0 19 8962.0 20 6754.0 21 6598.0 22 6800.0 23 7184.0 24 8095.0 25 9795.0 26 11502.0 27 13985.0 28 17040.0 29 21077.0 30 26176.0 31 32351.0 32 41230.0 33 52509.0 34 70556.0 35 100300.0 36 143867.0 37 205780.0 38 154524.0 39 12836.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.13885901902591 20.53067993366501 15.949550996219669 18.380910051089412 2 15.224781545348634 23.633433907373124 39.46299770530627 21.678786841971963 3 19.746372829455847 29.172381035209433 31.629041830611303 19.452204304723423 4 11.721913481433344 18.39520626167733 39.3528070988613 30.53007315802803 5 11.345523634038674 40.807912450028724 33.238081676225285 14.608482239707326 6 26.77404803682084 39.319586683028085 17.729776636046346 16.17658864410473 7 24.919148903636195 37.15451925135325 21.28906847304199 16.63726337196856 8 26.5413315391908 32.11967491360592 20.174546622220863 21.164446924982418 9 27.6988592923331 17.00491605247867 21.88809290804 33.40813174714823 10-11 24.08492821823339 28.088211964073757 26.606130168602572 21.22072964909028 12-13 27.50157689514125 26.71160021407546 27.35850949959861 18.428313391184677 14-15 23.65988829933637 26.39501590262699 29.584381403101013 20.360714394935624 16-17 19.129981039175508 29.955665693140464 29.66704830422949 21.24730496345454 18-19 18.99929088045506 27.63320361478944 34.50454333465855 18.862962170096946 20-21 21.28542593311148 25.412390642411914 34.234253176315875 19.067930248160728 22-23 20.368837732040735 25.877463760359642 34.37480886265635 19.37888964494327 24-25 20.524366368338082 26.09330999460495 34.635361040666446 18.746962596390524 26-27 20.197037508517713 25.561977886919184 35.532026121303694 18.708958483259412 28-29 19.16716802052118 26.70625570145656 35.14362294622126 18.982953331800996 30-31 19.60245099936866 26.264175903795262 34.73088663530511 19.402486461530962 32-33 19.676057334105536 26.42298333839852 34.590025593534904 19.310933733961033 34-35 20.406979355717965 25.847881621992023 34.52386072253554 19.22127829975448 36-37 20.490782535894542 25.58621619012758 34.785234854429156 19.137766419548726 38-39 20.096784845100764 26.29872751869251 34.58373771851733 19.020749917689397 40-41 19.740594619598667 26.651078641634072 34.36557407131306 19.24275266745419 42-43 19.4942079001165 27.202130475878278 33.06829813282311 20.235363491182113 44-45 20.233848100515786 26.59349575625285 32.76487222885366 20.407783914377706 46-47 20.92525074902161 27.492557568343745 31.85145718750149 19.730734495133152 48-49 20.903212118518816 27.596332256749672 31.07040513090311 20.4300504938284 50-51 20.3696509489275 28.02390751606394 31.4368162941152 20.16962524089336 52-53 20.741785284903578 28.836321168552896 30.14825766423218 20.27363588231134 54-55 20.032303307027163 28.098620658347134 30.621480265594347 21.247595769031356 56-57 21.05359481746492 28.31911181662534 29.661526787373948 20.965766578535792 58-59 21.72965234352974 27.539807463857663 29.968729362765195 20.761810829847402 60-61 20.88119190848617 28.072485880754677 31.010588657487826 20.03573355327133 62-63 21.075090216826204 27.918476100186552 30.929558243371357 20.07687543961589 64-65 20.714933194821878 28.6650862726003 30.53613777621257 20.083842756365257 66-67 20.937204632791364 28.137164111635784 29.85310125414888 21.072530001423974 68-69 20.795742989082235 28.398469754426742 30.143601486283984 20.66218577020704 70-71 21.413628526975316 28.5323271825101 29.67194016613721 20.382104124377367 72-73 21.10137160157803 27.96664271078626 30.191146900516834 20.74083878711887 74-75 20.55558157159609 29.65428823458016 29.344787611947286 20.44534258187646 76-77 20.810860532848476 28.536327211302066 29.696924808203697 20.955887447645765 78-79 20.69075421686632 28.6407639410205 29.8386746097317 20.82980723238148 80-81 20.852075688451524 29.264930128627554 29.208541783047277 20.67445239987365 82-83 20.704916755905405 28.72832764929151 29.631179991790514 20.935575603012566 84-85 20.27975770643026 28.82005172344586 29.275499028035025 21.624691542088854 86-87 20.53704885946167 29.023852485024104 28.95250910786851 21.486589547645714 88-89 20.616692597930342 28.48017309596352 28.81700874272236 22.08612556338378 90-91 20.311755607152193 27.97132356841235 29.506543847770068 22.210376976665398 92-93 20.754791813205298 28.961130309795408 29.287640677084926 20.996437199914368 94-95 20.295021335767157 28.602638658594277 29.1970297171637 21.905310288474862 96-97 19.70625521753944 28.648144192600856 29.45748102571748 22.188119564142227 98-99 20.201779825553583 28.990092594318185 28.426842747351838 22.38128483277639 100-101 19.861586040670748 28.650819894605455 28.98808624996894 22.499507814754853 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2795.0 1 1923.0 2 1118.5 3 1189.0 4 1023.0 5 995.5 6 1625.5 7 3330.5 8 5322.5 9 5994.5 10 4917.5 11 3750.0 12 3576.5 13 3612.0 14 3685.0 15 3750.0 16 3848.5 17 3977.5 18 4086.0 19 4251.5 20 4514.0 21 4964.0 22 5452.0 23 6012.5 24 6694.5 25 7473.0 26 8700.5 27 10227.0 28 11175.0 29 12476.5 30 14785.5 31 16652.5 32 19314.5 33 22608.5 34 25616.5 35 27546.0 36 30543.0 37 34168.0 38 34300.0 39 33413.0 40 32532.5 41 31565.0 42 30030.5 43 30114.5 44 32115.5 45 32720.0 46 32248.5 47 38040.5 48 58582.0 49 56820.5 50 34446.5 51 25742.5 52 22426.5 53 21731.5 54 21333.0 55 19815.0 56 18189.0 57 16627.0 58 15940.0 59 15297.0 60 13736.5 61 11881.5 62 9938.0 63 7355.5 64 4882.5 65 3551.5 66 2457.5 67 1708.0 68 1228.5 69 859.0 70 638.5 71 470.0 72 346.5 73 311.5 74 239.0 75 173.0 76 148.0 77 71.5 78 19.5 79 8.0 80 5.0 81 4.0 82 3.0 83 2.0 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01557769350744671 2 0.003918315544817885 3 0.002771491482919967 4 2.867060154744793E-4 5 0.0016246674210220497 6 3.822746872993058E-4 7 0.0 8 0.0 9 0.0 10-11 1.911373436496529E-4 12-13 7.645493745986116E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002867060154744793 22-23 0.0 24-25 0.0036793938652558184 26-27 0.0018158047646717023 28-29 0.003297119177956512 30-31 0.017441282608030826 32-33 9.556867182482645E-4 34-35 0.0023892167956206614 36-37 0.0037271782011682316 38-39 0.002532569803357901 40-41 0.004969570934890976 42-43 0.002771491482919967 44-45 4.7784335912413227E-4 46-47 2.867060154744793E-4 48-49 2.3892167956206614E-4 50-51 2.3892167956206614E-4 52-53 0.0013379614055475703 54-55 0.0033926878497813387 56-57 7.167650386861983E-4 58-59 0.0018158047646717023 60-61 0.0011946083978103307 62-63 0.0 64-65 4.3005902321171906E-4 66-67 9.556867182482645E-5 68-69 0.0 70-71 3.822746872993058E-4 72-73 0.0 74-75 4.7784335912413227E-4 76-77 0.0010034710541606776 78-79 4.3005902321171906E-4 80-81 0.005017355270803389 82-83 0.001672451756934463 84-85 0.0032493348420440994 86-87 0.001911373436496529 88-89 0.0011468240618979172 90-91 3.3449035138689257E-4 92-93 0.0 94-95 2.867060154744793E-4 96-97 0.001672451756934463 98-99 0.00281927581883238 100-101 0.0013379614055475703 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1046368.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.9531872105919 #Duplication Level Percentage of deduplicated Percentage of total 1 71.62127477611271 28.614981993912743 2 14.303740277588078 11.429600262443204 3 5.454958318653819 6.538289127934549 4 2.6043359721041295 4.162060906110205 5 1.4777569992426596 2.952055102125225 6 0.8955368960397772 2.146773195688176 7 0.5890779898219696 1.6474880246297408 8 0.44323943230675145 1.4167062414454492 9 0.3316405167064968 1.1925086085532886 >10 1.952072235280269 14.940356113947123 >50 0.19567760308585347 5.392622082773911 >100 0.1169702500464028 8.30661131252897 >500 0.006016988162788456 1.6411480731811743 >1k 0.0067390267423230715 5.445492200401353 >5k 4.813590530230766E-4 1.6934586463229424 >10k+ 4.813590530230766E-4 2.4798481080019514 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14646 1.399698767546408 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11143 1.0649217101440411 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9329 0.8915601394538059 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8282 0.7914997400532127 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4077 0.38963347502981743 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3684 0.35207498700266066 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3341 0.3192949325667451 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2894 0.27657573626104776 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2819 0.2694080858741858 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2699 0.2579398452552066 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2684 0.2565063151778342 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2577 0.24628046729257774 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2543 0.24303113245053368 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2498 0.23873054221841644 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2371 0.2265933208966635 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2134 0.20394354567417963 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2127 0.20327456497140584 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1937 0.18511651732468884 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1874 0.17909569099972478 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1766 0.1687742744426435 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1694 0.161893330071256 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1598 0.15271873757607268 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1265 0.12089436985840546 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1251 0.11955640845285789 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1233 0.11783617236001101 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1214 0.11602036759533932 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1101 0.10522110767913392 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1089 0.104074283617236 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1065 0.10178063549344017 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1058 0.10111165479066639 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 9.556867182482645E-5 0.0 16-17 0.0 0.0 0.0 9.556867182482645E-5 0.0 18-19 0.0 0.0 0.0 9.556867182482645E-5 0.0 20-21 0.0 0.0 0.0 1.4335300773723968E-4 0.0 22-23 0.0 0.0 0.0 9.079023823358513E-4 0.0 24-25 0.0 0.0 0.0 0.002006942108321355 0.0 26-27 0.0 0.0 0.0 0.0024370011315330746 0.0 28-29 0.0 0.0 0.0 0.004730649255328909 0.0 30-31 0.0 0.0 0.0 0.009031239487446099 0.0 32-33 0.0 0.0 0.0 0.0140963790941619 0.0 34-35 0.0 0.0 0.0 0.02016498975503838 0.0 36-37 0.0 0.0 0.0 0.02623360041591486 0.0 38-39 0.0 0.0 0.0 0.030295268968469985 0.0 40-41 0.0 0.0 0.0 0.03440472185693752 0.0 42-43 0.0 0.0 0.0 0.03927872412000367 0.0 44-45 0.0 0.0 0.0 0.044535001070369125 0.0 46-47 0.0 0.0 0.0 0.05361402489372764 0.0 48-49 0.0 0.0 0.0 0.06455663781767026 0.0 50-51 0.0 0.0 0.0 0.07497362304657634 0.0 52-53 0.0 0.0 0.0 0.09566424049665127 0.0 54-55 0.0 0.0 0.0 0.13618535735037768 0.0 56-57 0.0 0.0 0.0 0.21163682375607817 0.0 58-59 0.0 0.0 0.0 0.2890474479341876 0.0 60-61 0.0 0.0 0.0 0.35709234227346404 0.0 62-63 0.0 0.0 0.0 0.4380867916450044 0.0 64-65 0.0 0.0 0.0 0.5340377381571302 0.0 66-67 0.0 0.0 0.0 0.6933029297532034 0.0 68-69 0.0 0.0 0.0 0.8849181167619804 0.0 70-71 0.0 0.0 0.0 1.0414118168751338 0.0 72-73 0.0 0.0 0.0 1.2039741276491636 0.0 74-75 0.0 0.0 0.0 1.382926465641151 0.0 76-77 0.0 0.0 0.0 1.5715790238233585 0.0 78-79 0.0 0.0 0.0 1.7359093550261475 0.0 80-81 0.0 0.0 0.0 1.9147183400103978 0.0 82-83 0.0 0.0 0.0 2.093718462338298 0.0 84-85 0.0 0.0 0.0 2.4230481054466497 0.0 86-87 0.0 0.0 0.0 2.8456049879201197 0.0 88-89 0.0 0.0 0.0 3.308539634239579 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 10075 0.0 24.16071 12-13 GTACATG 9715 0.0 24.103811 1 GTCTATA 180 1.9512117E-7 23.749344 1 CGTCACT 160 1.8597366E-6 23.749344 1 CCCTTAC 100 0.0016538133 23.749342 1 TACATGG 9835 0.0 23.615402 2 CTTTGCG 245 2.9831426E-10 23.263552 2 AGTACTT 10395 0.0 23.188488 12-13 ACATGGG 9745 0.0 22.711414 3 GTACTTT 10925 0.0 22.259192 14-15 TAGGACG 110 0.0028792545 21.588247 5 ATGGGAT 2740 0.0 20.800358 5 CATGGGA 7230 0.0 20.75816 4 CATGGGG 2270 0.0 20.713306 4 ACTTTTT 12105 0.0 20.187449 16-17 TACTTTT 11950 0.0 20.071707 14-15 TCGCCCG 60 0.004766862 19.790173 94-95 TTTGCGT 295 3.0195224E-9 19.319653 3 GTATAGG 395 0.0 19.239973 1 GGTTACT 150 7.7218603E-4 18.999475 8 >>END_MODULE