##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936982_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 997873 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.815613810575094 33.0 31.0 34.0 30.0 34.0 2 31.887547814200804 33.0 31.0 34.0 30.0 34.0 3 31.859275679370022 33.0 31.0 34.0 30.0 34.0 4 35.49378227489871 37.0 35.0 37.0 33.0 37.0 5 35.524987648728846 37.0 35.0 37.0 33.0 37.0 6 35.630456982000716 37.0 35.0 37.0 33.0 37.0 7 35.58525483703838 37.0 35.0 37.0 33.0 37.0 8 35.54559347732627 37.0 35.0 37.0 33.0 37.0 9 37.14491924323035 39.0 37.0 39.0 33.0 39.0 10-11 36.96142094234436 39.0 37.0 39.0 33.0 39.0 12-13 36.900359564794314 39.0 37.0 39.0 33.0 39.0 14-15 37.90692452847206 40.0 37.5 41.0 33.0 41.0 16-17 37.901254969319744 40.0 37.5 41.0 33.0 41.0 18-19 38.00631793825467 40.0 38.0 41.0 33.0 41.0 20-21 37.9870609787017 40.0 38.0 41.0 33.0 41.0 22-23 37.88116173100184 40.0 37.5 41.0 33.0 41.0 24-25 37.70167145518518 40.0 37.0 41.0 32.5 41.0 26-27 37.72684600144507 40.0 37.0 41.0 32.5 41.0 28-29 37.66428343085743 40.0 37.0 41.0 33.0 41.0 30-31 37.42870335202977 39.5 36.5 41.0 32.0 41.0 32-33 37.10280065699743 39.0 35.5 41.0 31.5 41.0 34-35 36.865056475122586 39.0 35.0 41.0 31.0 41.0 36-37 36.61649278014336 39.0 35.0 41.0 30.5 41.0 38-39 36.39535792630926 39.0 35.0 41.0 30.0 41.0 40-41 36.192984979050436 39.0 35.0 41.0 30.0 41.0 42-43 35.86590678372899 39.0 35.0 40.0 29.5 41.0 44-45 35.42243602141755 38.0 35.0 40.0 27.5 41.0 46-47 34.77852893103632 38.0 34.5 40.0 24.0 41.0 48-49 34.22638502093954 38.0 34.0 40.0 21.0 41.0 50-51 33.45670340814914 37.5 33.5 39.5 19.0 40.5 52-53 33.649643291280555 38.0 33.5 39.5 19.0 40.5 54-55 34.020086223397165 38.0 34.0 40.0 18.5 41.0 56-57 33.891065796950116 38.0 34.0 40.0 16.0 41.0 58-59 33.65036382385334 38.0 34.0 40.0 13.5 41.0 60-61 33.339393389739975 38.0 33.0 40.0 10.0 41.0 62-63 33.034885200822146 37.0 33.0 40.0 8.0 41.0 64-65 32.72555475496381 37.0 33.0 40.0 7.0 41.0 66-67 32.32668385656291 36.0 32.0 39.0 3.5 41.0 68-69 31.93150430966666 36.0 32.0 39.0 2.0 41.0 70-71 31.47982609009363 35.0 31.5 38.5 2.0 40.0 72-73 31.090943937755604 35.0 31.0 37.5 2.0 40.0 74-75 30.68493936603155 35.0 31.0 37.0 2.0 39.0 76-77 30.2669277553356 35.0 30.5 36.5 2.0 39.0 78-79 29.82107141890802 35.0 30.0 36.0 2.0 39.0 80-81 29.38177653869781 34.0 29.0 36.0 2.0 37.5 82-83 28.975008342745017 34.0 29.0 35.0 2.0 37.0 84-85 28.615736671901132 34.0 29.0 35.0 2.0 36.5 86-87 28.332468159775843 34.0 29.0 35.0 2.0 36.0 88-89 27.95023715442747 34.0 28.0 35.0 2.0 36.0 90-91 27.56169171828479 34.0 27.0 35.0 2.0 35.5 92-93 27.20859067236011 34.0 26.0 35.0 2.0 35.0 94-95 26.786564021674103 34.0 25.0 35.0 2.0 35.0 96-97 26.31446386464009 33.5 24.5 35.0 2.0 35.0 98-99 25.755287997570832 33.0 22.0 35.0 2.0 35.0 100-101 23.66906961106273 31.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3658756331441744 1101 2 0.3466301234673672 1101 3 0.3418788191999056 1101 4 0.21516041794625096 1101 5 0.32435806635362496 1101 6 0.20304510381923535 1101 7 0.22411989228930196 1101 8 0.21597619080372965 1101 9 0.18880019786340085 1101 10-11 0.3724374012380096 1101 12-13 0.37675711222660624 1101 14-15 0.46359872107824884 1101 16-17 0.6890997474441392 1101 18-19 0.5241083441730865 1101 20-21 0.5393688119534232 1101 22-23 0.5629005777475271 1101 24-25 0.4303452671206571 1101 26-27 0.5100324492948332 1101 28-29 0.5057121780989817 1101 30-31 0.4258367649999215 1101 32-33 0.5804440761248983 1101 34-35 0.5094231206686857 1101 36-37 0.4143124807195093 1101 38-39 0.5287925566978515 1101 40-41 0.7093823022374934 1101 42-43 0.5085694319845828 1101 44-45 0.6246866082788856 1101 46-47 0.6551029810537443 1101 48-49 0.5367316041312407 1101 50-51 0.3369006827178751 1101 52-53 0.4530057950326025 1101 54-55 0.4741106792979721 1101 56-57 0.48281684650786616 1101 58-59 0.35058422157910485 1101 60-61 0.41799484707513557 1101 62-63 0.40362246999711004 1101 64-65 0.5229642844301949 1101 66-67 0.6596901157171402 1101 68-69 0.542715497117932 1101 70-71 0.5637723039976947 1101 72-73 0.4095316039438224 1101 74-75 0.34749837110727455 1101 76-77 0.46353761013085304 1101 78-79 0.3858203665536877 1101 80-81 0.10580414635308699 1101 82-83 0.11384248313622436 1101 84-85 0.38073128098914566 1101 86-87 0.4253213998676273 1101 88-89 0.5059751143124736 1101 90-91 0.6759879306633465 1101 92-93 0.532272967783161 1101 94-95 0.4984045641273944 1101 96-97 0.701164658594422 1101 98-99 0.46771045418560675 1101 100-101 0.3383980697328077 1102 1 0.3717390174390367 1102 2 0.3809755384617013 1102 3 0.4087390171226346 1102 4 0.38608324033664587 1102 5 0.31923765825288797 1102 6 0.30419709989754296 1102 7 0.2933545502138699 1102 8 0.3315740951512893 1102 9 0.476722621957272 1102 10-11 0.6093185097278564 1102 12-13 0.5267789419395257 1102 14-15 0.533764014132565 1102 16-17 0.6434220306023306 1102 18-19 0.5231336666400708 1102 20-21 0.4432127563144661 1102 22-23 0.4347505884708909 1102 24-25 0.392962841188492 1102 26-27 0.4460426671323816 1102 28-29 0.5377941965431674 1102 30-31 0.5563305760848181 1102 32-33 0.6309741168736949 1102 34-35 0.4328046580932323 1102 36-37 0.5217740297865774 1102 38-39 0.5022694554998921 1102 40-41 0.35050182183061196 1102 42-43 0.33045237876604006 1102 44-45 0.4604792949420755 1102 46-47 0.9334126719143683 1102 48-49 1.0360135214693926 1102 50-51 0.962434936218294 1102 52-53 0.9883565257535238 1102 54-55 1.10047599379903 1102 56-57 1.3361269203459116 1102 58-59 1.435480741080319 1102 60-61 1.2619372932285131 1102 62-63 1.4659137473033965 1102 64-65 1.4614887490277582 1102 66-67 1.1952744626640452 1102 68-69 1.150284279862504 1102 70-71 1.2440091372340056 1102 72-73 1.2897649903833575 1102 74-75 1.3856276030077588 1102 76-77 1.161414383316309 1102 78-79 1.1539830706806526 1102 80-81 1.410455024137331 1102 82-83 1.4148378414498985 1102 84-85 1.5160927350778124 1102 86-87 1.2787770051251393 1102 88-89 1.3356229282773633 1102 90-91 1.646512073559883 1102 92-93 1.6051325069847309 1102 94-95 1.3659001215893234 1102 96-97 1.5133276384651282 1102 98-99 1.7509337453403582 1102 100-101 1.5050487790069909 1103 1 0.3965103574779896 1103 2 0.34220146235260174 1103 3 0.37960753250555257 1103 4 0.2514009347116257 1103 5 0.23369248132409126 1103 6 0.28482594749619494 1103 7 0.1833202372029632 1103 8 0.37342625018031583 1103 9 0.21246384653555594 1103 10-11 0.5335280583136566 1103 12-13 0.35482795945859635 1103 14-15 0.6090316406664655 1103 16-17 0.6031313418377877 1103 18-19 0.5471191340707406 1103 20-21 0.5924659437240578 1103 22-23 0.5360870391300523 1103 24-25 0.5783016915157333 1103 26-27 0.649999035784198 1103 28-29 0.5789997120419557 1103 30-31 0.5141485601393612 1103 32-33 0.6632668464774483 1103 34-35 0.6353761715661363 1103 36-37 0.6222264905677051 1103 38-39 0.6061905953796085 1103 40-41 0.5804415124154687 1103 42-43 0.5234102687027544 1103 44-45 0.631320617824521 1103 46-47 0.7630567959047028 1103 48-49 0.866546307588699 1103 50-51 0.8502476499737739 1103 52-53 0.9771823704030496 1103 54-55 0.8928257435532672 1103 56-57 0.989626716635776 1103 58-59 0.879509978157806 1103 60-61 0.9208223259529547 1103 62-63 1.0695195102445965 1103 64-65 0.9580490378850044 1103 66-67 0.9437933591157517 1103 68-69 0.9982884811505528 1103 70-71 0.811697905516521 1103 72-73 0.7614366657030338 1103 74-75 0.8104683726956949 1103 76-77 1.0180071162840854 1103 78-79 1.1113071978107314 1103 80-81 0.994815318227495 1103 82-83 0.9897695331411427 1103 84-85 0.9339484896089623 1103 86-87 0.8600643597714139 1103 88-89 1.0231820322517642 1103 90-91 1.1272343661773476 1103 92-93 1.3479942419970854 1103 94-95 1.2490158214738294 1103 96-97 0.9978816440793068 1103 98-99 1.2176575069135254 1103 100-101 1.5264322698886232 1104 1 0.18908912651481202 1104 2 0.1432330910763575 1104 3 0.2598747430618573 1104 4 0.14599204035448565 1104 5 0.16054262816878406 1104 6 0.23468405725950703 1104 7 0.14829770817119936 1104 8 0.3566856794815436 1104 9 0.25721088005248305 1104 10-11 0.41179008870651757 1104 12-13 0.3586415176143305 1104 14-15 0.43514660065920197 1104 16-17 0.45321487954259965 1104 18-19 0.349994922999727 1104 20-21 0.40574885166192587 1104 22-23 0.36372962436741574 1104 24-25 0.5466853539078045 1104 26-27 0.18320768186783454 1104 28-29 0.190795731408393 1104 30-31 0.32913800438654306 1104 32-33 0.42971909511428663 1104 34-35 0.4081673768486951 1104 36-37 0.2977162252013059 1104 38-39 0.48790285684300017 1104 40-41 0.46158676342769667 1104 42-43 0.31983700186929553 1104 44-45 0.1514329739731366 1104 46-47 0.3584755716575714 1104 48-49 0.22796179688292995 1104 50-51 0.25683267814538624 1104 52-53 0.22243090539667065 1104 54-55 0.37271063492672596 1104 56-57 0.36456512994235624 1104 58-59 0.3219295163470619 1104 60-61 0.35756487843583074 1104 62-63 0.31046729849096977 1104 64-65 0.3503996265024014 1104 66-67 0.10361736567765689 1104 68-69 0.22733042616154364 1104 70-71 0.22445261294188512 1104 72-73 0.4179109066384328 1104 74-75 0.5240028571152919 1104 76-77 0.5584864327887651 1104 78-79 0.45608533432813303 1104 80-81 0.43011281980328775 1104 82-83 0.3906349121986388 1104 84-85 0.4537732118151254 1104 86-87 0.23691987394805025 1104 88-89 0.12331167396171594 1104 90-91 0.20401749018473936 1104 92-93 0.38649039816167274 1104 94-95 0.6252231271007211 1104 96-97 0.5897359609280493 1104 98-99 0.6988677164046209 1104 100-101 0.93047263222482 1105 1 0.16468384897766342 1105 2 0.155166411082579 1105 3 0.025964456751026432 1105 4 0.07751010969990091 1105 5 0.18900434603378358 1105 6 0.06994982212479073 1105 7 0.03312923123821321 1105 8 0.15859201208188978 1105 9 0.06636383089764308 1105 10-11 0.18317871845707856 1105 12-13 0.348297733602422 1105 14-15 0.35330146172403687 1105 16-17 0.2532706868325363 1105 18-19 0.07433189907755633 1105 20-21 0.2569419060143403 1105 22-23 0.2160166126617682 1105 24-25 0.32287868590842095 1105 26-27 0.42698142078966583 1105 28-29 0.45949811474061875 1105 30-31 0.1295158835984651 1105 32-33 0.36121911732613654 1105 34-35 0.2650577724418639 1105 36-37 0.45206502909282875 1105 38-39 0.21643370911193216 1105 40-41 0.16877618614153533 1105 42-43 0.5677046636271399 1105 44-45 0.5760042851390423 1105 46-47 0.4751641725327147 1105 48-49 0.47666934447183706 1105 50-51 0.5280172364626097 1105 52-53 0.330706765550822 1105 54-55 0.5547354122528603 1105 56-57 0.836437978168739 1105 58-59 0.7861767565238651 1105 60-61 0.8266806799506838 1105 62-63 0.7592884041971999 1105 64-65 0.7207073717768324 1105 66-67 0.7143809537809886 1105 68-69 0.4976001336313942 1105 70-71 0.396987374497602 1105 72-73 0.5860560500159693 1105 74-75 0.5940382294970696 1105 76-77 0.6494773037156669 1105 78-79 0.5095881441280028 1105 80-81 0.4697332191724719 1105 82-83 0.4494991089068421 1105 84-85 0.34103693318959927 1105 86-87 0.2662159246799298 1105 88-89 0.25452544087049134 1105 90-91 0.2967228387998304 1105 92-93 0.29813163225243144 1105 94-95 0.21695809522670473 1105 96-97 0.22655683340978072 1105 98-99 0.3404863499311972 1105 100-101 0.2892274355388267 1106 1 0.3489233947796073 1106 2 0.2668216136156083 1106 3 0.2740292018105279 1106 4 0.21247844685461104 1106 5 0.08406657990210675 1106 6 0.24539473969221604 1106 7 0.28691239533910107 1106 8 0.4859994801460843 1106 9 0.30016391824342037 1106 10-11 0.5440082304125369 1106 12-13 0.5003408059902341 1106 14-15 0.6599888730134822 1106 16-17 0.688248577431942 1106 18-19 0.5484748186102593 1106 20-21 0.6422582615116639 1106 22-23 0.38835666070194463 1106 24-25 0.5192319995889534 1106 26-27 0.45054158019572554 1106 28-29 0.5087665938322274 1106 30-31 0.6302760648409631 1106 32-33 0.7753814167038016 1106 34-35 0.6555166107429784 1106 36-37 0.7620865925822855 1106 38-39 0.5842290470309237 1106 40-41 0.5693957952691733 1106 42-43 0.378748445699415 1106 44-45 0.5803633854775043 1106 46-47 0.7378115685366495 1106 48-49 0.6750984852077266 1106 50-51 0.8077857701453297 1106 52-53 0.8528639333639063 1106 54-55 0.832974030005893 1106 56-57 0.9123892283052086 1106 58-59 0.9873531949996774 1106 60-61 1.015261583979509 1106 62-63 0.9235940052453415 1106 64-65 0.9023347328424549 1106 66-67 1.0178242882061426 1106 68-69 0.9595351156163261 1106 70-71 0.8996009240117395 1106 72-73 1.0964733990715452 1106 74-75 1.2445363733992423 1106 76-77 1.2109905693560243 1106 78-79 0.9774394379373703 1106 80-81 1.361036854940572 1106 82-83 1.3332664415850815 1106 84-85 1.1255221389980932 1106 86-87 1.381445480308276 1106 88-89 1.2549594402153978 1106 90-91 1.197251007813911 1106 92-93 1.1317706758161385 1106 94-95 1.065969996089244 1106 96-97 1.1257557088328625 1106 98-99 1.3794493917479898 1106 100-101 1.3913346524839056 1107 1 0.021177685551560188 1107 2 0.07577258721677538 1107 3 0.0900609111925057 1107 4 -0.03407225993622376 1107 5 0.018223433592559957 1107 6 -0.062383807730753915 1107 7 -0.1348506052509606 1107 8 -0.09607291781178873 1107 9 0.14103977447369687 1107 10-11 0.31620864602093945 1107 12-13 0.23158711810701504 1107 14-15 0.3129956202363431 1107 16-17 0.12759027424304747 1107 18-19 0.3037712602839804 1107 20-21 0.38829330602280976 1107 22-23 0.21336115029890834 1107 24-25 0.19786280078960772 1107 26-27 0.10838683020212869 1107 28-29 0.19487321534636948 1107 30-31 0.23709255577197297 1107 32-33 0.10818241211158153 1107 34-35 0.18735041869085478 1107 36-37 0.15957498376728552 1107 38-39 -0.13246968818547344 1107 40-41 0.07935885229988315 1107 42-43 -0.0014909139557843787 1107 44-45 0.13409616466630325 1107 46-47 0.027021142922862396 1107 48-49 -0.29686975410061933 1107 50-51 -0.30312395047346286 1107 52-53 -0.3002633916059452 1107 54-55 -0.3454027882808788 1107 56-57 -0.3723269008925598 1107 58-59 -0.13321685554035412 1107 60-61 -0.09027986141417443 1107 62-63 -0.1888255026948258 1107 64-65 -0.0813269824401388 1107 66-67 -0.17739108417385552 1107 68-69 -0.23061342590603573 1107 70-71 -0.227724045843825 1107 72-73 -0.30812523096001243 1107 74-75 0.029886177139719194 1107 76-77 -0.05724104253756224 1107 78-79 0.00414484341211363 1107 80-81 -0.24932256936482844 1107 82-83 -0.25047865192680874 1107 84-85 -0.08068120126204903 1107 86-87 -0.22291071148730524 1107 88-89 -0.3006435767937603 1107 90-91 -0.2842197841509915 1107 92-93 -0.13615692622598985 1107 94-95 -0.15563266175216128 1107 96-97 -0.09466939186238577 1107 98-99 -0.11170738497793664 1107 100-101 -0.22626585035095204 1108 1 0.19324109159409986 1108 2 0.2360866066962224 1108 3 0.21809987008094112 1108 4 0.23495933770938393 1108 5 0.21284475861627072 1108 6 0.22698529311375637 1108 7 0.17198074852525025 1108 8 0.2465033666422869 1108 9 0.07362192985764437 1108 10-11 0.24410911117923462 1108 12-13 0.28865933054435544 1108 14-15 0.542033389885141 1108 16-17 0.43857643992370043 1108 18-19 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-1.6915880539962522 2316 70-71 -1.6137387932341305 2316 72-73 -2.1063767518813883 2316 74-75 -2.5155827929681784 2316 76-77 -2.735396535140648 2316 78-79 -2.8235859780144317 2316 80-81 -3.049783876815404 2316 82-83 -2.6805627089555557 2316 84-85 -3.0851549547257235 2316 86-87 -3.3578741010134614 2316 88-89 -3.532051957728452 2316 90-91 -3.1908243755533334 2316 92-93 -3.3036427490199394 2316 94-95 -3.9968131237820153 2316 96-97 -4.3094701885723445 2316 98-99 -4.39142533726822 2316 100-101 -4.381886164390899 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1571.0 3 496.0 4 519.0 5 549.0 6 692.0 7 893.0 8 1149.0 9 1439.0 10 1961.0 11 3061.0 12 4536.0 13 6168.0 14 7103.0 15 9708.0 16 13145.0 17 18634.0 18 14991.0 19 7791.0 20 6676.0 21 6604.0 22 6995.0 23 7535.0 24 8560.0 25 9589.0 26 11180.0 27 12972.0 28 15442.0 29 18918.0 30 22528.0 31 28285.0 32 35811.0 33 46410.0 34 63632.0 35 92444.0 36 130391.0 37 182364.0 38 166432.0 39 30693.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.79752485372495 19.82041955574092 15.332813333386804 19.049242257147327 2 14.84455753361345 22.534220000541385 40.37393615474769 22.247286311097476 3 19.30035595678799 29.32749127149048 31.40198457687654 19.97016819484499 4 11.329061003021778 17.73234305101331 40.02211692897518 30.916479016989733 5 11.351901526736695 40.242873975281405 33.462859434931936 14.942365063049964 6 26.545604779634807 38.77015455797102 18.5116587831706 16.172581879223568 7 24.812754645158897 36.99099798339586 21.62680087964453 16.56944649180071 8 26.02754287215619 31.889704164322623 20.64030709710803 21.44244586641316 9 27.628355483443322 16.849794075677064 22.136939925452086 33.38491051542753 10-11 23.711000571640607 27.76909730927763 27.041007692076256 21.478894427005507 12-13 27.14748890090086 26.55409952133725 27.429190906510836 18.869220671251053 14-15 23.693852020286855 26.04102998653727 29.51546709653805 20.749650896637828 16-17 19.171765300413306 29.859895360193157 29.444484551691225 21.523854787702316 18-19 19.013319831359006 27.80591246108574 33.976699068062985 19.204068639492274 20-21 21.424213331194274 25.689182786911957 33.362678571876195 19.523925310017574 22-23 20.42285288111943 26.05628014166268 33.598023619134686 19.922843358083203 24-25 20.429250710640158 26.22813453450564 33.87974445019104 19.462870304663163 26-27 20.12130432974171 25.737755935047108 34.85928427137973 19.281655463831456 28-29 19.22987293179011 26.81327162427301 34.482368064308396 19.474487379628496 30-31 19.49876846982062 26.538310235356317 34.076427535160626 19.88649375966244 32-33 19.518870931209147 26.64535598098976 33.92668509427949 19.909087993521606 34-35 20.305543807040156 26.20735205389662 33.71550530086333 19.771598838199893 36-37 20.333309614746682 25.817714978087032 34.14128527346361 19.707690133702673 38-39 20.101875399321 26.550041967853875 33.712689325131855 19.63539330769327 40-41 19.549667918424287 26.891542067173983 33.86951454053279 19.689275473868932 42-43 19.35134078786163 27.39476512723893 32.43391267528706 20.819981409612385 44-45 19.892609970556933 26.88052809117129 32.31216265240144 20.914699285870334 46-47 20.69553165283848 27.440343702052733 31.527618127537355 20.336506517571436 48-49 20.52389931087769 27.647468817127795 30.830118548278396 20.99851332371612 50-51 20.130675787948242 28.095745395179257 31.164907653037194 20.608671163835307 52-53 20.396212753270245 28.898025450418285 29.86127292178719 20.844488874524277 54-55 19.750106986761704 28.14378218998644 30.442318851618634 21.663791971633227 56-57 20.69013907328248 28.39658012739071 29.46670615989042 21.44657463943639 58-59 21.291887008118454 27.783092308640562 29.73956905696949 21.185451626271497 60-61 20.790813897428137 28.083224998772156 30.70600723076815 20.41995387303156 62-63 20.782488596580027 28.05652247558701 30.676978772750463 20.4840101550825 64-65 20.43297466755932 28.691274158825863 30.47890700480671 20.396844168808105 66-67 20.617088377635998 28.362892107509108 29.607803536428477 21.412215978426413 68-69 20.35064505458154 28.4795858017823 30.07813831333514 21.091630830301025 70-71 21.065843823514378 28.528414449938307 29.491499770968417 20.9142419555789 72-73 20.673972495643717 28.051516668646226 30.105898938204152 21.168611897505905 74-75 20.136066354273684 29.734933720901097 29.336657729220438 20.792342195604782 76-77 20.35762418536675 28.5936960429987 29.659642055048486 21.389037716586063 78-79 20.288669508750562 28.6346217948236 29.85942461585502 21.217284080570824 80-81 20.497633479239123 29.255840366734283 29.131938231377212 21.11458792264938 82-83 20.303242185248397 28.820839003460968 29.608917826380065 21.26700098491057 84-85 20.119861254433527 28.77652411383311 29.144036481604086 21.95957815012928 86-87 20.238145953859345 29.09840235319236 29.11639511298601 21.54705657996228 88-89 20.413179111606272 28.46200763891708 28.74787814819659 22.37693510128005 90-91 19.956468855385488 27.998203826591084 29.587396315338687 22.457931002684738 92-93 20.41707892381087 28.826322706371666 29.378772340828647 21.37782602898882 94-95 19.941621614036073 28.477004739735545 29.374231616045837 22.207142030182546 96-97 19.294181925723937 28.502599010521358 29.71478553777212 22.488433525982586 98-99 19.74348549103282 28.833442040083963 28.741296148773 22.681776320110213 100-101 19.62392183622891 28.327397781576042 29.424375989920176 22.624304392274873 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1523.0 1 1095.5 2 777.5 3 930.5 4 855.5 5 860.0 6 1414.5 7 3036.5 8 5025.0 9 5608.0 10 4592.0 11 3532.0 12 3316.0 13 3467.0 14 3533.5 15 3496.0 16 3580.5 17 3670.0 18 3675.0 19 3760.5 20 4005.5 21 4393.0 22 4965.0 23 5594.0 24 6158.5 25 6969.5 26 8217.0 27 9561.0 28 10498.5 29 11748.5 30 13934.5 31 15980.0 32 18639.0 33 21791.0 34 24871.5 35 26766.0 36 29698.5 37 33505.5 38 33312.5 39 31969.0 40 31185.0 41 30243.0 42 28845.0 43 28871.5 44 30504.5 45 31220.0 46 30914.0 47 35912.5 48 54523.0 49 53364.5 50 33494.0 51 25347.5 52 21811.0 53 21114.5 54 20607.0 55 19027.5 56 17524.0 57 16086.5 58 15205.0 59 14423.0 60 13306.0 61 11905.0 62 9826.5 63 6900.0 64 4552.5 65 3395.0 66 2370.5 67 1642.5 68 1155.5 69 772.0 70 560.0 71 436.0 72 334.5 73 288.5 74 210.5 75 162.5 76 122.5 77 57.0 78 28.0 79 13.0 80 8.0 81 7.0 82 6.5 83 6.5 84 6.0 85 2.0 86 2.0 87 3.5 88 2.0 89 0.5 90 0.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043793148025850985 2 0.04309165595221035 3 0.028059682945625344 4 0.04479527955962332 5 0.06493812338844723 6 0.06433684446818383 7 0.06533897600195616 8 0.06654153384248296 9 0.06634110753572849 10-11 0.0744583729592844 12-13 0.07215347043160802 14-15 0.06769398510632114 16-17 0.05481659489734665 18-19 0.04890401884808989 20-21 0.055167340934166975 22-23 0.029262240786152145 24-25 0.018138580761279242 26-27 0.013478669129237888 28-29 0.018088474184590624 30-31 0.013077816515728955 32-33 0.00952024957083717 34-35 0.011123660024872905 36-37 0.00621321550938847 38-39 0.008568224613753454 40-41 0.007616199656669736 42-43 0.004559698478664119 44-45 0.0015031973006585007 46-47 0.0032068209080714683 48-49 9.52024957083717E-4 50-51 0.006012789202634003 52-53 0.0050607642455502855 54-55 0.00861833119044207 56-57 0.023399771313583992 58-59 0.00846801146037622 60-61 0.019240925448428807 62-63 0.014631120393076073 64-65 0.010121528491100571 66-67 0.017186555804195525 68-69 0.028360322405757046 70-71 0.019441351755183273 72-73 0.01768762157108169 74-75 0.019842204368692207 76-77 0.026205739608146525 78-79 0.036627907559378794 80-81 0.031216397277008194 82-83 0.0321183156574033 84-85 0.021996787166302725 86-87 0.025003181767619728 88-89 0.021896574012925493 90-91 0.01969188463862636 92-93 0.03166735646720575 94-95 0.024401902847356327 96-97 0.03788057197659422 98-99 0.054265422553771876 100-101 0.06348503266447734 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 997873.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.13970141786189 #Duplication Level Percentage of deduplicated Percentage of total 1 73.33194292903575 30.901861794212554 2 13.62972666639588 11.487052242579852 3 5.107834267536935 6.457278327777893 4 2.363248360487698 3.983463211488129 5 1.3353864599489333 2.8136393349851816 6 0.8534270488156137 2.1577896611410208 7 0.5527885876676062 1.630604222206015 8 0.40019738123103205 1.349135852262875 9 0.3153246565156893 1.1958918187734906 >10 1.8125199441790432 14.724547469621758 >50 0.1834362331392514 5.367156108131231 >100 0.10198641753054642 7.6917350255125765 >500 0.005732257654597196 1.8125730287098998 >1k 0.005493413585655646 4.704396464819225 >5k 7.165322068246495E-4 2.3713717102245515 >10k+ 2.3884406894154984E-4 1.3515037275538084 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13428 1.3456622235494897 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9679 0.9699631115382419 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7050 0.7065027313094954 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6832 0.6846562638732584 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4809 0.4819250545911153 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3895 0.390330232404324 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3094 0.31005949654916004 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2738 0.27438361394686495 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2500 0.25053288344308344 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2333 0.2337972868290855 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2112 0.2116501799327169 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2060 0.20643909595710075 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1992 0.19962460152744887 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1982 0.19862246999367655 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1956 0.19601692800586848 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1924 0.19281010709779703 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1907 0.19110648349038403 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1780 0.17837941301147542 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1637 0.16404893207853105 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1619 0.16224509531774084 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1591 0.1594391270231783 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1183 0.11855216044526708 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1181 0.11835173413851262 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1178 0.11805109467838092 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1160 0.11624725791759072 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1107 0.11093596078859734 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1003 0.10051379283736507 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.0021315337723337E-4 0.0 18-19 0.0 0.0 0.0 2.0042630675446674E-4 0.0 20-21 0.0 0.0 0.0 3.507460368203168E-4 0.0 22-23 0.0 0.0 0.0 8.01705227017867E-4 0.0 24-25 0.0 0.0 0.0 0.0013027709939040337 0.0 26-27 0.0 0.0 0.0 0.0024552222577422177 0.0 28-29 0.0 0.0 0.0 0.0049104445154844354 0.0 30-31 0.0 0.0 0.0 0.00997120876103472 0.0 32-33 0.0 0.0 0.0 0.015082079583273624 0.0 34-35 0.0 0.0 0.0 0.019641778061937742 0.0 36-37 0.0 0.0 0.0 0.023499984466961228 0.0 38-39 0.0 0.0 0.0 0.028711068442577363 0.0 40-41 0.0 0.0 0.0 0.03347119322799595 0.0 42-43 0.0 0.0 0.0 0.03953408900731857 0.0 44-45 0.0 0.0 0.0 0.04544666505657534 0.0 46-47 0.0 0.0 0.0 0.05416520940039464 0.0 48-49 0.0 0.0 0.0 0.06388588527798628 0.0 50-51 0.0 0.0 0.0 0.07280485592856005 0.0 52-53 0.0 0.0 0.0 0.09124407614997099 0.0 54-55 0.0 0.0 0.0 0.1288240086664335 0.0 56-57 0.0 0.0 0.0 0.20318216847234066 0.0 58-59 0.0 0.0 0.0 0.27708936908805026 0.0 60-61 0.0 0.0 0.0 0.3391714176052464 0.0 62-63 0.0 0.0 0.0 0.41939204688372167 0.0 64-65 0.0 0.0 0.0 0.5126904926779259 0.0 66-67 0.0 0.0 0.0 0.6674196014923743 0.0 68-69 0.0 0.0 0.0 0.8554695838047527 0.0 70-71 0.0 0.0 0.0 1.0201699013802357 0.0 72-73 0.0 0.0 0.0 1.186273203103 0.0 74-75 0.0 0.0 0.0 1.3679596501759241 0.0 76-77 0.0 0.0 0.0 1.5610202901571641 0.0 78-79 0.0 0.0 0.0 1.7170521699655166 0.0 80-81 0.0 0.0 0.0 1.8886671951240288 0.0 82-83 0.0 1.0021315337723337E-4 0.0 2.060382433435918 0.0 84-85 0.0 1.0021315337723337E-4 0.0 2.4012574746485775 0.0 86-87 0.0 1.0021315337723337E-4 0.0 2.8365333063425906 0.0 88-89 0.0 1.0021315337723337E-4 0.0 3.3164540978661616 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 8905 0.0 25.433578 12-13 GTACATG 9400 0.0 24.675135 1 TACCTGG 620 0.0 24.53283 2 TACATGG 9560 0.0 23.716457 2 AGTACTT 9230 0.0 23.5081 12-13 CATTCCG 185 2.5194095E-7 23.128492 9 GTACTTT 9860 0.0 22.943779 14-15 ACATGGG 9545 0.0 22.857363 3 ATGGGAT 2735 0.0 22.770296 5 GTATCAA 22345 0.0 21.377321 1 GTCGGGA 335 1.8189894E-12 21.283146 2 CATGGGA 7180 0.0 21.184338 4 GATTGGC 405 0.0 21.129732 9 GTACACG 135 3.778044E-4 21.125492 3 TGGGATA 815 0.0 20.997995 6 ACTTTTT 11065 0.0 20.895103 16-17 CATGGGG 2135 0.0 20.704962 4 ATGGGAG 2165 0.0 20.640713 5 TACTTTT 10705 0.0 19.978401 14-15 GTTCTAA 300 3.6852725E-9 19.01199 1 >>END_MODULE