##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936982_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 997873 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11791781118439 31.0 31.0 34.0 31.0 34.0 2 32.1454012684981 33.0 31.0 34.0 30.0 34.0 3 32.25983567047109 33.0 31.0 34.0 30.0 34.0 4 35.982636066914324 37.0 35.0 37.0 35.0 37.0 5 35.91102675390556 37.0 35.0 37.0 35.0 37.0 6 35.98253485162942 37.0 35.0 37.0 35.0 37.0 7 35.93112650607843 37.0 35.0 37.0 35.0 37.0 8 35.93595076728201 37.0 35.0 37.0 35.0 37.0 9 37.57306390693004 39.0 37.0 39.0 35.0 39.0 10-11 37.476107179971805 39.0 37.0 39.0 35.0 39.0 12-13 37.40181215445252 39.0 37.0 39.0 34.0 39.0 14-15 38.45349708830683 40.0 38.0 41.0 34.0 41.0 16-17 38.42334345152139 40.0 38.0 41.0 34.0 41.0 18-19 38.58441204441848 40.0 38.0 41.0 34.0 41.0 20-21 38.71340741757719 40.0 38.0 41.0 34.0 41.0 22-23 38.63289266269355 40.0 38.0 41.0 34.0 41.0 24-25 38.52069501830393 40.0 38.0 41.0 34.0 41.0 26-27 38.29238690695109 40.0 38.0 41.0 34.0 41.0 28-29 38.1306569072417 40.0 37.0 41.0 34.0 41.0 30-31 37.94015019947428 40.0 37.0 41.0 33.0 41.0 32-33 37.701802734416106 40.0 36.0 41.0 33.0 41.0 34-35 37.50360917671888 40.0 36.0 41.0 33.0 41.0 36-37 37.24595414446528 40.0 35.0 41.0 33.0 41.0 38-39 37.030420704839194 39.0 35.0 41.0 32.5 41.0 40-41 36.75674910534707 39.0 35.0 41.0 31.5 41.0 42-43 36.411107926559794 39.0 35.0 41.0 31.0 41.0 44-45 35.9615006118013 39.0 35.0 41.0 30.0 41.0 46-47 35.31020230029272 39.0 35.0 41.0 26.0 41.0 48-49 34.696234390548696 39.0 35.0 40.0 23.0 41.0 50-51 34.3799291092153 38.5 34.5 40.0 21.5 41.0 52-53 34.15440391713174 38.0 34.0 40.0 20.0 41.0 54-55 33.89337270374085 38.0 34.0 40.0 18.5 41.0 56-57 33.64571192927357 38.0 34.0 40.0 16.5 41.0 58-59 33.32396106518565 38.0 33.0 40.0 13.5 41.0 60-61 32.94982628049862 37.0 33.0 40.0 9.0 41.0 62-63 32.631486171085896 36.5 32.5 39.5 7.5 41.0 64-65 32.22190348872051 36.0 32.0 39.0 6.0 40.5 66-67 31.785244214444123 35.5 31.0 39.0 2.0 40.0 68-69 31.342726980287072 35.0 31.0 38.0 2.0 40.0 70-71 30.926095304713126 35.0 31.0 37.5 2.0 40.0 72-73 30.554543514054394 35.0 30.5 37.0 2.0 39.0 74-75 30.105378640368066 34.5 30.0 36.5 2.0 39.0 76-77 28.6416092027743 33.0 28.0 35.0 2.0 37.5 78-79 29.32510950792335 34.0 29.0 36.0 2.0 37.5 80-81 29.052774751897285 34.0 29.0 35.0 2.0 37.0 82-83 28.82721498627581 34.0 29.0 35.0 2.0 37.0 84-85 28.496624319928486 34.0 29.0 35.0 2.0 36.0 86-87 28.160106546624668 34.0 29.0 35.0 2.0 36.0 88-89 27.774812526243316 34.0 27.5 35.0 2.0 35.5 90-91 27.309038324516244 33.0 26.5 35.0 2.0 35.0 92-93 26.91777460658821 33.0 26.0 35.0 2.0 35.0 94-95 26.449122283096145 33.0 25.0 35.0 2.0 35.0 96-97 25.837253337849607 33.0 24.0 35.0 2.0 35.0 98-99 25.151586424324538 32.0 19.0 34.0 2.0 35.0 100-101 22.225462057796932 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2461829755409184 1101 2 0.23257403585699876 1101 3 0.2167576062437746 1101 4 0.22796907819242307 1101 5 0.26262974552552976 1101 6 0.27418252893819073 1101 7 0.3074005205706527 1101 8 0.17037082662066894 1101 9 0.37799633770370633 1101 10-11 0.23477530141293812 1101 12-13 0.1585692877074436 1101 14-15 0.3671245476050231 1101 16-17 0.3297609259396381 1101 18-19 0.383317179272197 1101 20-21 0.21240100729622924 1101 22-23 0.26335084695107014 1101 24-25 0.2917623871076742 1101 26-27 0.332294557545346 1101 28-29 0.34807937811380185 1101 30-31 0.42697370039231686 1101 32-33 0.5996962961348018 1101 34-35 0.5801279924166707 1101 36-37 0.6467780341526961 1101 38-39 0.5137699883413305 1101 40-41 0.8369965081969823 1101 42-43 0.9216750117290005 1101 44-45 1.0035812649428095 1101 46-47 1.2019148557590356 1101 48-49 1.046313468092137 1101 50-51 0.955612286074718 1101 52-53 0.8240277190840573 1101 54-55 0.9009916168404146 1101 56-57 0.8392444422297203 1101 58-59 0.969734582889636 1101 60-61 0.8162560943151647 1101 62-63 0.8859023123361212 1101 64-65 0.8847589766112591 1101 66-67 1.027631969692333 1101 68-69 0.984415476807726 1101 70-71 0.9605595591641034 1101 72-73 1.0568463444571776 1101 74-75 0.8939905119553977 1101 76-77 0.8394798951791103 1101 78-79 1.1219458947687109 1101 80-81 1.160914775292781 1101 82-83 1.0801153778928132 1101 84-85 1.1954968090584899 1101 86-87 0.8882113321905969 1101 88-89 0.5517789337559442 1101 90-91 0.6333227539058264 1101 92-93 0.8029933072867479 1101 94-95 1.0443979097989988 1101 96-97 0.8916970836006861 1101 98-99 1.0828108485175711 1101 100-101 1.453312220908355 1102 1 0.3519115517840632 1102 2 0.39756070824279277 1102 3 0.24538133858851552 1102 4 0.24409625733394336 1102 5 0.28887518809423796 1102 6 0.27521273819704817 1102 7 0.2599419587274596 1102 8 0.0893634428160226 1102 9 0.1568498565002372 1102 10-11 0.3553595717643674 1102 12-13 0.31745895957239156 1102 14-15 0.33674869356537585 1102 16-17 0.4270659138375521 1102 18-19 0.38997332682573926 1102 20-21 0.2604416948172954 1102 22-23 0.3215729891735677 1102 24-25 0.34265817129223564 1102 26-27 0.36679890816883187 1102 28-29 0.5643256632328288 1102 30-31 0.5033413127578896 1102 32-33 0.3846506452263796 1102 34-35 0.3145254923400742 1102 36-37 0.2240724518738304 1102 38-39 0.2189865237743973 1102 40-41 0.36402322470177495 1102 42-43 0.04463374208225446 1102 44-45 0.3637906769958761 1102 46-47 0.24160280575750903 1102 48-49 0.344202879532439 1102 50-51 0.26594846365369307 1102 52-53 0.27326214722217657 1102 54-55 0.15074459640474203 1102 56-57 0.4723022871391862 1102 58-59 0.34763372195639164 1102 60-61 0.3657693300147855 1102 62-63 0.23188028347494338 1102 64-65 0.28812036942353814 1102 66-67 0.32654393084311195 1102 68-69 0.2634815480721606 1102 70-71 0.3225479013314505 1102 72-73 0.5386912998786073 1102 74-75 0.3801611973317911 1102 76-77 0.43684998923836815 1102 78-79 0.3797088470650465 1102 80-81 0.5609346901186036 1102 82-83 0.6443866484586991 1102 84-85 0.44019640157051754 1102 86-87 0.4158374083571488 1102 88-89 0.40249242068875546 1102 90-91 0.24580705183162266 1102 92-93 0.5676690020077899 1102 94-95 0.9503233364358437 1102 96-97 0.9266629220148559 1102 98-99 1.2005706603990625 1102 100-101 1.5160343478117504 1103 1 0.2471615545874144 1103 2 0.1267589126033144 1103 3 0.15660950515987082 1103 4 0.13858935548485363 1103 5 0.13755032461740768 1103 6 0.24139965813709807 1103 7 0.11450226253590756 1103 8 0.19087276676000187 1103 9 0.1939213643664388 1103 10-11 0.08992385633061417 1103 12-13 0.28548768083337706 1103 14-15 0.411540217728394 1103 16-17 0.42516103712890896 1103 18-19 0.35998728751155085 1103 20-21 0.2958260528686978 1103 22-23 0.24444347063968053 1103 24-25 0.3463590612625964 1103 26-27 0.3056984809382115 1103 28-29 0.2439751135892223 1103 30-31 0.2335417149452539 1103 32-33 0.2500986954026132 1103 34-35 0.2519191986311782 1103 36-37 0.4628576479401687 1103 38-39 0.43200285422588536 1103 40-41 0.5031497754218606 1103 42-43 0.37168616847561964 1103 44-45 0.7846717140113171 1103 46-47 0.8355187874721892 1103 48-49 0.7982153151955416 1103 50-51 0.5173029492014862 1103 52-53 0.6258068419746721 1103 54-55 0.8565352907759873 1103 56-57 0.7226167296275179 1103 58-59 0.6711441149113853 1103 60-61 0.6039994626693357 1103 62-63 0.6969921184734673 1103 64-65 0.5170746822849352 1103 66-67 0.6527950662000812 1103 68-69 0.7552686653680851 1103 70-71 0.6991602951311897 1103 72-73 0.6842010798342777 1103 74-75 0.6318296581428164 1103 76-77 0.940097203642356 1103 78-79 0.8011412096915258 1103 80-81 1.0593307063705595 1103 82-83 1.0121736274559971 1103 84-85 1.0287724676847354 1103 86-87 0.9446866890173915 1103 88-89 0.8666473786945623 1103 90-91 0.7278109428822042 1103 92-93 0.6872524614918945 1103 94-95 0.9859431759346791 1103 96-97 1.024952130610302 1103 98-99 1.251833196048338 1103 100-101 1.343198441002599 1104 1 0.017781054792479267 1104 2 0.0030130103548131615 1104 3 -0.009986973319882964 1104 4 0.08827956409515281 1104 5 -0.05849491510965521 1104 6 0.018597580566762417 1104 7 0.012079806720059594 1104 8 0.1414355108703873 1104 9 0.04342963418634582 1104 10-11 0.016952914239993788 1104 12-13 0.12398603898029847 1104 14-15 0.2065452960501446 1104 16-17 0.20352050524007836 1104 18-19 0.08330270221363634 1104 20-21 0.07657329557394377 1104 22-23 0.004509970506184402 1104 24-25 0.08497687695116696 1104 26-27 0.11935159715608279 1104 28-29 0.09571839547874816 1104 30-31 0.3843664949534542 1104 32-33 0.3139135196981826 1104 34-35 0.12340875710619059 1104 36-37 0.24493296124096986 1104 38-39 0.2612501340895861 1104 40-41 0.3502317359568323 1104 42-43 0.4392608776075093 1104 44-45 0.6079392355976267 1104 46-47 0.6307413115494853 1104 48-49 0.5156018924617243 1104 50-51 0.884678929427686 1104 52-53 0.5721734334986976 1104 54-55 0.5954462453628508 1104 56-57 0.5319209307345645 1104 58-59 0.789608012034769 1104 60-61 0.8076511751234747 1104 62-63 0.7349253929037687 1104 64-65 0.7098004336425987 1104 66-67 0.6233201564125501 1104 68-69 0.9864671768280289 1104 70-71 1.0209180825793354 1104 72-73 0.9837752715003241 1104 74-75 0.8927797034210805 1104 76-77 0.6919544381176905 1104 78-79 1.0508212341335295 1104 80-81 1.22310314250619 1104 82-83 1.2220046528365955 1104 84-85 0.8626081282381506 1104 86-87 0.909453595424889 1104 88-89 0.8550690803314644 1104 90-91 0.9947641425001592 1104 92-93 0.8593993116937924 1104 94-95 0.6915271278874258 1104 96-97 0.7314210042137645 1104 98-99 0.8946358791638112 1104 100-101 1.0792536997492874 1105 1 0.1175511620757419 1105 2 0.16972687721694513 1105 3 0.12040773137348282 1105 4 0.15313540914524282 1105 5 0.2125397131749267 1105 6 0.22677820738535814 1105 7 0.24759767615876882 1105 8 0.19876153650286454 1105 9 -0.00196307143991703 1105 10-11 0.018800663068319068 1105 12-13 0.16352869685106697 1105 14-15 0.3376008034800151 1105 16-17 0.2295083775663258 1105 18-19 0.3682558351960168 1105 20-21 0.30608424601929585 1105 22-23 0.20502056556182424 1105 24-25 0.213365573337299 1105 26-27 0.3341943927403648 1105 28-29 0.174049896333905 1105 30-31 0.3053728031207541 1105 32-33 0.05245220164660935 1105 34-35 -0.24098230484268157 1105 36-37 -0.2801164601401709 1105 38-39 -0.12645921265971793 1105 40-41 -0.15246283990776277 1105 42-43 -0.07829065390000522 1105 44-45 -0.12394421879032791 1105 46-47 -0.2251856447597973 1105 48-49 0.050971497757039685 1105 50-51 -0.21425129202452808 1105 52-53 -0.03700200685135968 1105 54-55 -0.11047987800179726 1105 56-57 -0.17153273533239144 1105 58-59 -0.14866940294934494 1105 60-61 -0.13397051253339498 1105 62-63 -0.13762436165521308 1105 64-65 -0.1831925585166161 1105 66-67 -0.36460769947692384 1105 68-69 -0.4776259685929958 1105 70-71 -0.38860191919761533 1105 72-73 -0.20386216436293836 1105 74-75 -0.34062387490903134 1105 76-77 -0.48145491529515994 1105 78-79 -0.3477177809927454 1105 80-81 -0.0905768623804164 1105 82-83 -0.27444422657523404 1105 84-85 -0.35353267272140343 1105 86-87 -0.3541664374486615 1105 88-89 -0.5801222748139132 1105 90-91 -0.3400185144821357 1105 92-93 -0.3937617131612754 1105 94-95 -0.28742852614069747 1105 96-97 -0.17103562713605314 1105 98-99 -0.15080402665791226 1105 100-101 -0.1342480549873919 1106 1 0.07660236853933355 1106 2 -0.0021122973654357224 1106 3 0.011345606687378051 1106 4 0.037465030282923806 1106 5 -0.004268890384416579 1106 6 0.03849346014221311 1106 7 0.09107949731828313 1106 8 0.06407161402224659 1106 9 0.13827876718875132 1106 10-11 0.1776212220813136 1106 12-13 0.17011784413788433 1106 14-15 0.2509592487251311 1106 16-17 0.08081310515667184 1106 18-19 0.04190743471561831 1106 20-21 0.12377859038003436 1106 22-23 0.18872375368280103 1106 24-25 0.21568433084357963 1106 26-27 0.14192312950202535 1106 28-29 0.22622944650313315 1106 30-31 0.04448460571929047 1106 32-33 0.16113764765599825 1106 34-35 0.30055607270946183 1106 36-37 0.42232376259156723 1106 38-39 0.42673300264763725 1106 40-41 0.42810681835187125 1106 42-43 0.615350846263766 1106 44-45 0.5732579866906207 1106 46-47 0.7330698962381348 1106 48-49 0.57653924532773 1106 50-51 0.28356642825061584 1106 52-53 0.4280446685498802 1106 54-55 0.6190320774518625 1106 56-57 0.5459650810231125 1106 58-59 0.48065284838813227 1106 60-61 0.6100026286392222 1106 62-63 0.7470996802707575 1106 64-65 0.8026478716613568 1106 66-67 0.6143528857398017 1106 68-69 0.685559149869718 1106 70-71 0.9113871625798744 1106 72-73 0.8049679480947525 1106 74-75 0.8044670192933872 1106 76-77 0.7539733519829284 1106 78-79 0.7022303574503148 1106 80-81 0.9859776195499279 1106 82-83 1.0988143169338827 1106 84-85 0.7312172999830437 1106 86-87 0.8235071622892995 1106 88-89 0.6633704645681142 1106 90-91 0.4170128208164776 1106 92-93 0.7196008264172775 1106 94-95 0.9126902095291349 1106 96-97 0.8425029687805292 1106 98-99 0.9885070335014916 1106 100-101 0.8794420312665672 1107 1 0.12372517825738782 1107 2 -0.013349117191495452 1107 3 -0.05928473272614809 1107 4 -0.021161409594959935 1107 5 0.07610772266363597 1107 6 0.05642619315229069 1107 7 0.034133449446350994 1107 8 0.0977692460805315 1107 9 0.07336945704739861 1107 10-11 0.08600265823923792 1107 12-13 0.16629270752586223 1107 14-15 0.07480798778975384 1107 16-17 0.15963503491371966 1107 18-19 0.19601349182328676 1107 20-21 0.17146580657337296 1107 22-23 0.11694022922091563 1107 24-25 0.26021343625931337 1107 26-27 0.29636398226889327 1107 28-29 0.1459464736202989 1107 30-31 0.10437839468257692 1107 32-33 0.23869992477958135 1107 34-35 0.21664678822293837 1107 36-37 0.30157541091502793 1107 38-39 0.3647060137561837 1107 40-41 0.22059123303669992 1107 42-43 -0.0446327346387676 1107 44-45 -0.1311931280121641 1107 46-47 0.22434468522707363 1107 48-49 0.2686029206400775 1107 50-51 0.1020982017696852 1107 52-53 0.1165038388723616 1107 54-55 0.32427464396202765 1107 56-57 0.27133643482900993 1107 58-59 0.24837649445396437 1107 60-61 0.3247387686120291 1107 62-63 0.4939441349771414 1107 64-65 0.3640206621382731 1107 66-67 0.2940316871456865 1107 68-69 0.33392621931736954 1107 70-71 0.2097375563816648 1107 72-73 0.24086291718067088 1107 74-75 0.17216629326142296 1107 76-77 0.1473813409000364 1107 78-79 0.34252769704342967 1107 80-81 0.42222919090886535 1107 82-83 0.33123946893880785 1107 84-85 0.24666536175989506 1107 86-87 0.357076520148663 1107 88-89 0.214176026537384 1107 90-91 0.11897669880205797 1107 92-93 0.203653778006597 1107 94-95 -0.06178835585221876 1107 96-97 4.918858897440259E-4 1107 98-99 0.06388864924329951 1107 100-101 0.18602506315552958 1108 1 0.1238456075879526 1108 2 0.06753423194818708 1108 3 -0.010894722339116925 1108 4 0.07119283328088954 1108 5 -0.029644283237402647 1108 6 0.09705603855113765 1108 7 0.1274586537998701 1108 8 0.10355738078074239 1108 9 0.11814658751752205 1108 10-11 0.16778922735815627 1108 12-13 0.2517523712269636 1108 14-15 0.2985581571434963 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-0.7580207207786991 2316 88-89 -0.6497524656477403 2316 90-91 -0.8666177634445198 2316 92-93 -0.7506953605249578 2316 94-95 -0.6682218919750724 2316 96-97 -0.8102520884751847 2316 98-99 -1.2849531800935843 2316 100-101 -1.9887660842822505 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 17.0 5 26.0 6 72.0 7 180.0 8 370.0 9 791.0 10 1392.0 11 2524.0 12 4052.0 13 5607.0 14 7160.0 15 9953.0 16 14149.0 17 20305.0 18 16798.0 19 8329.0 20 6435.0 21 6180.0 22 6299.0 23 6733.0 24 7443.0 25 8953.0 26 10492.0 27 13013.0 28 15834.0 29 19208.0 30 24285.0 31 29848.0 32 37831.0 33 49263.0 34 66848.0 35 95811.0 36 139963.0 37 200700.0 38 149531.0 39 11476.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.20812408658155 20.434611768338033 15.960891155603402 18.39637298947702 2 15.208157947484466 23.550110242533574 39.50651433152936 21.735217478452597 3 19.784458912292237 29.19275823638361 31.628093905597293 19.394688945726863 4 11.840207802411335 18.394060955774844 39.38174373240629 30.38398750940753 5 11.383850946616464 40.81960920689866 33.180633322543116 14.615906523941765 6 26.759016427942235 39.314321561962295 17.764785699182163 16.16187631091331 7 24.86218186081796 37.25444019429326 21.279962480195376 16.603415464693402 8 26.54699199897382 32.13578495037439 20.18545464748986 21.131768403161928 9 27.676350626835163 17.05111888322126 21.883912734123683 33.388617755819894 10-11 24.100461681997608 28.152430219075974 26.57617752960547 21.170930569320944 12-13 27.537085148653762 26.77411192311643 27.263653422656702 18.425149505573106 14-15 23.707976866795676 26.33932374159838 29.578563604787384 20.374135786818563 16-17 19.12635087466128 30.01807847098626 29.600539949011495 21.255030705340968 18-19 19.050124495715934 27.695273371947298 34.43302461201883 18.82157752031793 20-21 21.31495993157326 25.495274616414903 34.16937622731949 19.02038922469235 22-23 20.40515175778882 25.925794164187227 34.2778088995293 19.39124517849466 24-25 20.517248118145677 26.13359737587679 34.63373617860554 18.715418327372 26-27 20.274494436749723 25.614052142818487 35.473863447310976 18.637589973120814 28-29 19.188134004092102 26.721642293281406 35.18466829462138 18.905555408005107 30-31 19.547402040194395 26.3917068984875 34.669470864676725 19.39142019664138 32-33 19.591656553367653 26.606361006192248 34.495084986786736 19.306897453653367 34-35 20.36066860189805 25.908398713787694 34.4848010174246 19.246131666889653 36-37 20.505709338658875 25.621587489319264 34.73437856285535 19.138324609166517 38-39 20.105744011497446 26.422222378122783 34.52605616228714 18.94597744809263 40-41 19.766476323154674 26.848858565332755 34.226953944219915 19.157711167292653 42-43 19.468414132060115 27.29733169771535 33.04068599742631 20.19356817279823 44-45 20.17631021297428 26.667107617577308 32.70382580033843 20.452756369109988 46-47 20.913036223507323 27.570573229449618 31.76490572447766 19.751484822565395 48-49 20.893794787073965 27.641375702575495 31.045306066614454 20.419523443736086 50-51 20.40710076249967 28.13346675532515 31.360648763600047 20.098783718575135 52-53 20.73882882557855 28.95961861468217 30.070322177191443 20.23123038254783 54-55 20.047502129578593 28.166006914866966 30.55434183494513 21.232149120609307 56-57 21.002192185797142 28.45331896929687 29.56103671598041 20.983452128925578 58-59 21.7122774245789 27.66604467141815 29.865342371305108 20.756335532697843 60-61 20.970153516529656 28.087692521994285 30.959601071479035 19.982552889997024 62-63 21.009798345981075 27.995560555080935 30.897183758446094 20.097457340491896 64-65 20.673488409384742 28.784613996933224 30.494292386173445 20.047605207508596 66-67 21.001757249090936 28.31551114302364 29.731732651612212 20.95099895627321 68-69 20.829685942760477 28.447040891027243 30.095319030384537 20.62795413582774 70-71 21.40263634459487 28.662906473205112 29.60080014351271 20.3336570386873 72-73 21.08408346859404 27.988367996656528 30.258091964586836 20.6694565701626 74-75 20.560200677275784 29.75112770727415 29.27784642736523 20.410825188084836 76-77 20.810586134861175 28.712355317796007 29.5664056177709 20.910652929571917 78-79 20.676807588904282 28.725736578228002 29.725864362868286 20.871591469999423 80-81 21.01311591065788 29.31653558773069 29.074971539435918 20.59537696217551 82-83 20.709856066512867 28.801479622618064 29.510724357650965 20.977939953218108 84-85 20.304961244738138 28.932134573381457 29.19669955189307 21.566204629987336 86-87 20.49158560257668 29.093582049018263 28.944765516253067 21.47006683215199 88-89 20.628123556286898 28.555530279267355 28.74027544918581 22.07607071525994 90-91 20.324873279578622 28.10201971006127 29.38726241800001 22.185844592360095 92-93 20.725075498424893 29.125531069474587 29.135702750412506 21.01369068168802 94-95 20.23940160122181 28.738564311934606 29.115219223480576 21.906814863363007 96-97 19.694729315536065 28.81000853318849 29.38333056910805 22.11193158216739 98-99 20.111106490164314 29.124240946855384 28.392533507570867 22.37211905540944 100-101 19.84987949271695 28.896343693786235 28.895091019326262 22.358685794170555 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2628.0 1 1772.0 2 992.5 3 1084.5 4 953.5 5 957.0 6 1517.0 7 3083.0 8 4993.0 9 5667.5 10 4788.5 11 3711.0 12 3421.0 13 3515.0 14 3658.0 15 3646.0 16 3635.0 17 3765.5 18 3858.5 19 4023.0 20 4347.5 21 4677.5 22 5133.5 23 5765.0 24 6436.0 25 7197.5 26 8288.5 27 9689.0 28 10786.0 29 12067.0 30 14011.0 31 15819.0 32 18593.0 33 21724.0 34 24585.5 35 26436.0 36 29224.0 37 32592.0 38 32895.0 39 32076.0 40 31093.0 41 30172.5 42 28598.5 43 28559.5 44 30618.5 45 31309.5 46 30706.0 47 36277.0 48 56109.5 49 54088.0 50 32714.0 51 24626.5 52 21128.5 53 20410.5 54 20058.0 55 18844.0 56 17312.0 57 15738.0 58 15203.0 59 14656.5 60 13177.0 61 11512.0 62 9556.5 63 6807.0 64 4519.5 65 3314.5 66 2273.0 67 1575.5 68 1131.5 69 785.0 70 581.0 71 447.5 72 342.0 73 307.0 74 227.5 75 175.5 76 135.0 77 52.0 78 14.0 79 4.5 80 3.0 81 3.0 82 1.5 83 2.0 84 3.0 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02475264888417664 2 0.007315560196538036 3 0.010522381104609504 4 2.0042630675446677E-4 5 0.005611936589125069 6 0.0 7 0.0 8 1.0021315337723338E-4 9 3.006394601317001E-4 10-11 0.0 12-13 5.010657668861669E-5 14-15 0.0 16-17 1.0021315337723338E-4 18-19 0.004760124785418585 20-21 0.001753730184101584 22-23 0.0 24-25 0.005411510282370602 26-27 0.007515986503292503 28-29 0.009770782454280254 30-31 0.032168422234091915 32-33 0.0012025578405268004 34-35 0.005962682625945385 36-37 0.01658527688393212 38-39 0.00861833119044207 40-41 0.011574619215070456 42-43 0.008518118037064837 44-45 0.0029562880246283845 46-47 0.0026556485644966847 48-49 0.0026556485644966847 50-51 0.003657780098269018 52-53 0.0037078866749576346 54-55 0.0023049025276763676 56-57 0.0010522381104609505 58-59 9.019183803951003E-4 60-61 0.0 62-63 5.010657668861669E-5 64-65 0.00831769173031037 66-67 6.51385496952017E-4 68-69 0.0013528775705926505 70-71 0.005211083975616136 72-73 0.01087312714142982 74-75 0.005211083975616136 76-77 0.003958419558400718 78-79 0.010071421914411955 80-81 1.0021315337723338E-4 82-83 0.005110870822238903 84-85 8.518118037064837E-4 86-87 0.0 88-89 0.0012025578405268004 90-91 7.014920736406336E-4 92-93 4.5095919019755016E-4 94-95 0.001002131533772334 96-97 4.5095919019755016E-4 98-99 1.5031973006585005E-4 100-101 8.017052270178671E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 997873.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.368854272682356 #Duplication Level Percentage of deduplicated Percentage of total 1 72.02684895302062 29.076413691049925 2 14.18206231416391 11.450272136931664 3 5.341553131050693 6.468971398115267 4 2.5452590373169945 4.109967646547101 5 1.4204795038333258 2.867156504378983 6 0.9078830732861346 2.199011968927377 7 0.5838039278490798 1.6497246981011344 8 0.443030790712787 1.4307716342876586 9 0.3085927509961802 1.1211782215113855 >10 1.91761403102669 14.896178204891687 >50 0.19467065715305146 5.39610972684687 >100 0.11401250088562531 8.095771952238877 >500 0.0069701965568537225 1.9632204268392195 >1k 0.00622338978290511 5.156970856066146 >5k 4.978711826324088E-4 1.6394337655331197 >10k+ 4.978711826324088E-4 2.4788471677335706 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13899 1.3928626187901667 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10768 1.079095235566049 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8644 0.8662424977928053 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7670 0.7686348864033801 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3889 0.38972895348406056 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3646 0.36537715721339287 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3200 0.32068209080714677 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2720 0.2725797771860748 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2655 0.2660659222165546 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2654 0.2659657090631774 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2639 0.2644625117625189 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2527 0.25323863858426876 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2505 0.2510339492099696 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2421 0.24261604432628203 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2308 0.23129195799465463 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1966 0.1970190595396408 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1963 0.19671842007950913 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1818 0.18218751283981027 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1796 0.17998282346551114 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1692 0.16956065551427887 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1549 0.1552301745813345 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1536 0.15392740358743046 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1190 0.11925365251890772 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1161 0.11634747107096795 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1157 0.11594661845745903 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1129 0.11314065016289648 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1119 0.11213851862912415 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1069 0.10712786096026249 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1008 0.10101485860425125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.0021315337723337E-4 0.0 4 0.0 0.0 0.0 1.0021315337723337E-4 0.0 5 0.0 0.0 0.0 1.0021315337723337E-4 0.0 6 0.0 0.0 0.0 1.0021315337723337E-4 0.0 7 0.0 0.0 0.0 1.0021315337723337E-4 0.0 8 0.0 0.0 0.0 1.0021315337723337E-4 0.0 9 0.0 0.0 0.0 1.0021315337723337E-4 0.0 10-11 0.0 0.0 0.0 1.0021315337723337E-4 0.0 12-13 0.0 0.0 0.0 1.0021315337723337E-4 0.0 14-15 0.0 0.0 0.0 1.0021315337723337E-4 0.0 16-17 0.0 0.0 0.0 1.0021315337723337E-4 0.0 18-19 0.0 0.0 0.0 1.0021315337723337E-4 0.0 20-21 0.0 0.0 0.0 2.505328834430834E-4 0.0 22-23 0.0 0.0 0.0 7.014920736406336E-4 0.0 24-25 0.0 0.0 0.0 0.0012025578405268004 0.0 26-27 0.0 0.0 0.0 0.0023550091043649845 0.0 28-29 0.0 0.0 0.0 0.0049104445154844354 0.0 30-31 0.0 0.0 0.0 0.010171635067789188 0.0 32-33 0.0 0.0 0.0 0.015382719043405324 0.0 34-35 0.0 0.0 0.0 0.02009273725213529 0.0 36-37 0.0 0.0 0.0 0.024151369963913243 0.0 38-39 0.0 0.0 0.0 0.029262240786152145 0.0 40-41 0.0 0.0 0.0 0.034172685301636585 0.0 42-43 0.0 0.0 0.0 0.040486113964402284 0.0 44-45 0.0 0.0 0.0 0.04629847686028182 0.0 46-47 0.0 0.0 0.0 0.05506712778078974 0.0 48-49 0.0 0.0 0.0 0.06458737735162692 0.0 50-51 0.0 0.0 0.0 0.07410762692246409 0.0 52-53 0.0 0.0 0.0 0.09259695372056365 0.0 54-55 0.0 0.0 0.0 0.12997645993027168 0.0 56-57 0.0 0.0 0.0 0.20413419342942438 0.0 58-59 0.0 0.0 0.0 0.2784422466586429 0.0 60-61 0.0 0.0 0.0 0.34062450832921626 0.0 62-63 0.0 0.0 0.0 0.4214965231046436 0.0 64-65 0.0 0.0 0.0 0.5155966741258657 0.0 66-67 0.0 0.0 0.0 0.6714281276274636 0.0 68-69 0.0 0.0 0.0 0.8605804546269916 0.0 70-71 0.0 0.0 0.0 1.023426828864996 0.0 72-73 0.0 0.0 0.0 1.1883275727472333 0.0 74-75 0.0 0.0 0.0 1.368661142249565 0.0 76-77 0.0 0.0 0.0 1.5597676257399489 0.0 78-79 0.0 0.0 0.0 1.7155990792415468 0.0 80-81 0.0 0.0 0.0 1.8877652767436337 0.0 82-83 0.0 0.0 0.0 2.0604325400126067 0.0 84-85 0.0 0.0 0.0 2.403512270599565 0.0 86-87 0.0 0.0 0.0 2.8427465218519794 0.0 88-89 0.0 0.0 0.0 3.327577757891034 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATATAG 325 0.0 27.774786 3 CTTTGCG 180 6.668415E-9 26.39946 2 TAATACC 305 0.0 24.916769 4 GAGTACT 9735 0.0 24.198898 12-13 GTACATG 9480 0.0 23.712954 1 AGTACTT 9980 0.0 23.652426 12-13 TACATGG 9665 0.0 23.058895 2 ACATGGG 9535 0.0 22.471718 3 ATACCTT 405 0.0 22.28059 6 TTTGCGT 235 4.636604E-9 22.23849 3 GTACTTT 10565 0.0 22.164045 14-15 GTAGATA 285 8.367351E-11 21.663397 6 AGGCTTA 490 0.0 21.323313 6 CATGGGA 7145 0.0 21.27254 4 CATGGGG 2115 0.0 21.110043 4 TACTTTT 11405 0.0 20.552444 14-15 GATATAG 565 0.0 20.18811 1 GTATAGG 495 0.0 20.16262 1 ACTTTTT 11790 0.0 20.102884 16-17 GTATAGT 215 1.0728163E-6 19.894678 1 >>END_MODULE