##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936981_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1024822 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.778543005517054 33.0 31.0 34.0 30.0 34.0 2 31.852461207897566 33.0 31.0 34.0 30.0 34.0 3 31.81849140631251 33.0 31.0 34.0 30.0 34.0 4 35.444581595633196 37.0 35.0 37.0 33.0 37.0 5 35.45727453157719 37.0 35.0 37.0 33.0 37.0 6 35.59725884104752 37.0 35.0 37.0 33.0 37.0 7 35.55636686175745 37.0 35.0 37.0 33.0 37.0 8 35.52016155000576 37.0 35.0 37.0 33.0 37.0 9 37.08129216585905 39.0 37.0 39.0 33.0 39.0 10-11 36.96035116342155 39.0 37.0 39.0 33.0 39.0 12-13 36.86051089847798 39.0 37.0 39.0 33.0 39.0 14-15 37.881839480417085 40.0 37.5 41.0 33.0 41.0 16-17 37.8599493375435 40.0 37.5 41.0 33.0 41.0 18-19 37.94069994594183 40.0 38.0 41.0 33.0 41.0 20-21 37.87425182129189 40.0 37.5 41.0 33.0 41.0 22-23 37.74648524329103 40.0 37.0 41.0 32.5 41.0 24-25 37.82086596501637 40.0 37.0 41.0 33.0 41.0 26-27 37.730481488492636 40.0 37.0 41.0 32.5 41.0 28-29 37.63040996387666 40.0 37.0 41.0 32.0 41.0 30-31 37.36761749845339 39.0 36.5 41.0 32.0 41.0 32-33 37.03654927392269 39.0 35.5 41.0 31.5 41.0 34-35 36.78147229470093 39.0 35.0 41.0 31.0 41.0 36-37 36.51545683055204 39.0 35.0 41.0 30.5 41.0 38-39 36.29768974514599 39.0 35.0 40.5 30.0 41.0 40-41 36.07623616589027 39.0 35.0 40.0 30.0 41.0 42-43 35.745891481642666 38.0 35.0 40.0 29.0 41.0 44-45 35.287797295530346 38.0 35.0 40.0 26.5 41.0 46-47 34.65563385641604 38.0 34.5 40.0 23.5 41.0 48-49 34.150964265013826 38.0 34.0 40.0 21.0 41.0 50-51 33.33876761037527 37.5 33.0 39.5 18.5 40.5 52-53 33.5029200192814 38.0 33.5 39.5 18.0 40.5 54-55 33.90493617428197 38.0 34.0 40.0 17.5 41.0 56-57 33.79430135184451 38.0 34.0 40.0 15.0 41.0 58-59 33.54018209991589 38.0 34.0 40.0 12.5 41.0 60-61 33.228448452511756 37.5 33.0 40.0 9.0 41.0 62-63 32.90796206560749 37.0 33.0 40.0 8.0 41.0 64-65 32.56390768348064 36.5 32.5 39.5 6.5 41.0 66-67 32.160850372064615 36.0 32.0 39.0 2.0 41.0 68-69 31.761722035631553 35.5 31.5 39.0 2.0 41.0 70-71 31.32488763902414 35.0 31.0 38.5 2.0 40.0 72-73 30.91997439555357 35.0 31.0 37.5 2.0 40.0 74-75 30.490854021478853 35.0 30.5 37.0 2.0 39.0 76-77 30.070894750503015 35.0 30.0 36.5 2.0 39.0 78-79 29.6378771142696 34.5 29.5 36.0 2.0 38.5 80-81 29.184675972998235 34.0 29.0 35.5 2.0 37.5 82-83 28.759646065365498 34.0 29.0 35.0 2.0 37.0 84-85 28.361702812781147 34.0 28.5 35.0 2.0 36.5 86-87 28.04629194142983 34.0 28.0 35.0 2.0 36.0 88-89 27.65409749205228 34.0 27.0 35.0 2.0 36.0 90-91 27.231229911145547 34.0 26.0 35.0 2.0 35.5 92-93 26.869557835409466 34.0 25.0 35.0 2.0 35.0 94-95 26.460310180694794 33.0 25.0 35.0 2.0 35.0 96-97 25.941842095505365 33.0 23.5 35.0 2.0 35.0 98-99 25.32658305539889 33.0 19.0 35.0 2.0 35.0 100-101 22.932889809157103 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3485007881658717 1101 2 0.4045471577064639 1101 3 0.41404845874970064 1101 4 0.27898287586065607 1101 5 0.41040242970858287 1101 6 0.2577472205785796 1101 7 0.2222370836680767 1101 8 0.16222589433771617 1101 9 0.3890365613434241 1101 10-11 0.434981523066611 1101 12-13 0.5170459072810445 1101 14-15 0.48879542927367226 1101 16-17 0.7575777544766424 1101 18-19 0.7634887875728893 1101 20-21 0.6814306301202677 1101 22-23 0.5973527067173237 1101 24-25 0.7384673518224503 1101 26-27 0.6146372368785435 1101 28-29 0.5448814028706224 1101 30-31 0.5511247530829095 1101 32-33 0.5601911720778716 1101 34-35 0.6932758970572479 1101 36-37 0.8516756922736946 1101 38-39 1.0490290333862973 1101 40-41 0.9385005327999565 1101 42-43 0.8174087236975041 1101 44-45 0.8792865109594175 1101 46-47 0.8853494400741582 1101 48-49 0.8038889718570275 1101 50-51 0.8374398490594643 1101 52-53 0.9518088011146588 1101 54-55 0.9849395861675774 1101 56-57 0.8233112008379067 1101 58-59 0.8273030653752613 1101 60-61 0.8531231813342686 1101 62-63 0.9345063781701626 1101 64-65 0.9383884510447658 1101 66-67 0.9251513695988436 1101 68-69 1.0438442459526591 1101 70-71 0.6180723350945776 1101 72-73 0.778251896798114 1101 74-75 0.6414027456780573 1101 76-77 0.726056299381014 1101 78-79 0.6688009732859399 1101 80-81 0.7720137559726759 1101 82-83 0.8015297937461732 1101 84-85 0.6707224243296821 1101 86-87 0.5801429176049702 1101 88-89 0.6287368032883762 1101 90-91 0.6341588710326356 1101 92-93 0.6388304698177976 1101 94-95 0.9570979282207155 1101 96-97 1.0242002121028513 1101 98-99 0.763616285713244 1101 100-101 1.2490426716028047 1102 1 0.40103188055125116 1102 2 0.3633410523449392 1102 3 0.17478268325577062 1102 4 0.2326707402601471 1102 5 0.32011626367336987 1102 6 0.22365425091638258 1102 7 0.23748993386336537 1102 8 0.16413130133783227 1102 9 0.3584838494004714 1102 10-11 0.42708454168798227 1102 12-13 0.349580683837047 1102 14-15 0.28012985698477877 1102 16-17 0.4683622045901856 1102 18-19 0.43408939373408373 1102 20-21 0.5663477890490611 1102 22-23 0.5846140151543651 1102 24-25 0.19185203968014264 1102 26-27 0.39950613700969484 1102 28-29 0.2656471730877499 1102 30-31 0.27043268546685795 1102 32-33 0.5941325534691586 1102 34-35 0.37315984568225247 1102 36-37 0.20218807022354923 1102 38-39 0.277969536908806 1102 40-41 0.24660657167371625 1102 42-43 0.20853812347510825 1102 44-45 0.05371232241238744 1102 46-47 0.012971413465614035 1102 48-49 0.12386350886519182 1102 50-51 0.109950467434075 1102 52-53 0.13899920845536684 1102 54-55 0.18488547491138263 1102 56-57 0.2187397591087219 1102 58-59 0.12237107382198786 1102 60-61 0.10590142016638993 1102 62-63 0.256702450882635 1102 64-65 0.3178647423513681 1102 66-67 0.22863732417800975 1102 68-69 0.2864166605331455 1102 70-71 -0.018760064883547756 1102 72-73 0.0023367785544614605 1102 74-75 0.040888688744189494 1102 76-77 0.04224736584287925 1102 78-79 0.03972164177102755 1102 80-81 -0.039010357116008976 1102 82-83 0.11713849261031584 1102 84-85 -0.07098027653196937 1102 86-87 0.010998038554181022 1102 88-89 -0.008698591805345046 1102 90-91 0.10027217654920051 1102 92-93 -0.07622915330305702 1102 94-95 -0.08993712992489833 1102 96-97 0.1844798636800924 1102 98-99 0.3178397939839215 1102 100-101 0.29240123446924216 1103 1 0.3839080976422409 1103 2 0.4771063042646979 1103 3 0.35122000427857714 1103 4 0.47073286336606657 1103 5 0.5241371426960697 1103 6 0.43911585434943134 1103 7 0.29176452414431964 1103 8 0.2944280822582286 1103 9 0.5332024366860821 1103 10-11 0.4359084801672637 1103 12-13 0.609947660201243 1103 14-15 0.7203937277463197 1103 16-17 0.6848333107970674 1103 18-19 0.7168933982179055 1103 20-21 0.7512060764128705 1103 22-23 0.8296044958011137 1103 24-25 0.5600479126927951 1103 26-27 0.529929105776823 1103 28-29 0.5876638206727307 1103 30-31 0.7784833779802369 1103 32-33 0.7924533340284938 1103 34-35 0.534674763020007 1103 36-37 0.7480969589138979 1103 38-39 0.8471900183937322 1103 40-41 0.7296315430511839 1103 42-43 0.5926838639905299 1103 44-45 0.6040184430756099 1103 46-47 0.6438733434028094 1103 48-49 0.8543732432904321 1103 50-51 0.5476026182000027 1103 52-53 0.6358178009911271 1103 54-55 0.6544944675915687 1103 56-57 0.7075509498522408 1103 58-59 0.6755753701036866 1103 60-61 0.7403352281932243 1103 62-63 0.7846014784993542 1103 64-65 0.7835342794077675 1103 66-67 0.5704907933766634 1103 68-69 0.8108616412503586 1103 70-71 0.8651144178153594 1103 72-73 0.8608774391340255 1103 74-75 0.853506183906223 1103 76-77 0.546079515742985 1103 78-79 0.693926414250658 1103 80-81 0.7704639553674717 1103 82-83 0.8634152603224372 1103 84-85 0.823493064212979 1103 86-87 0.8190852058028177 1103 88-89 0.871292621099343 1103 90-91 0.7171667949599154 1103 92-93 0.8369522265288047 1103 94-95 0.8418242589888081 1103 96-97 0.769676463424215 1103 98-99 0.7343798890208681 1103 100-101 1.0625597429841598 1104 1 0.3805939719834228 1104 2 0.43012500947500243 1104 3 0.44898403853166613 1104 4 0.3975227584900267 1104 5 0.4864140182951928 1104 6 0.41251999593742994 1104 7 0.2851122186888446 1104 8 0.34161606445460535 1104 9 0.43682463348812917 1104 10-11 0.4181159907599934 1104 12-13 0.5326122706845666 1104 14-15 0.6439514594589397 1104 16-17 0.6475075251176534 1104 18-19 0.5106835107199998 1104 20-21 0.6945995629086923 1104 22-23 0.6153035090469174 1104 24-25 0.6908681231575926 1104 26-27 0.6483413901359256 1104 28-29 0.42926465027321115 1104 30-31 0.21709543640862705 1104 32-33 0.4045117624202206 1104 34-35 0.44818721949982887 1104 36-37 0.37370588495900847 1104 38-39 0.34495743804400547 1104 40-41 0.5725571320212026 1104 42-43 0.4631313956796248 1104 44-45 0.49290347142385116 1104 46-47 0.5352470841241441 1104 48-49 0.5478023655460333 1104 50-51 0.28725666624413293 1104 52-53 0.3678783140747939 1104 54-55 0.3573296486390447 1104 56-57 0.37036895446351537 1104 58-59 0.4018196817455362 1104 60-61 0.4166404769842842 1104 62-63 0.628673202593049 1104 64-65 0.6780475305650313 1104 66-67 0.5150803050598469 1104 68-69 0.4651364179032278 1104 70-71 0.48560025794621353 1104 72-73 0.5327892441921627 1104 74-75 0.4473667661232632 1104 76-77 0.5372258112275823 1104 78-79 0.5970938096686105 1104 80-81 0.49854751766055827 1104 82-83 0.4457793009899653 1104 84-85 0.4860683002107997 1104 86-87 0.5911575499587336 1104 88-89 0.5548145566912694 1104 90-91 0.42046924990740564 1104 92-93 0.6327740227849112 1104 94-95 0.844381570199463 1104 96-97 0.41026309930158433 1104 98-99 0.589995817492774 1104 100-101 0.6225240359528676 1105 1 0.026855835953742968 1105 2 0.31482333441728017 1105 3 0.17466224246057038 1105 4 0.20031020028693547 1105 5 0.27788229520350427 1105 6 0.1711755702954889 1105 7 0.08789703280683625 1105 8 0.2669225394058188 1105 9 0.3231783961491601 1105 10-11 0.2451836237117888 1105 12-13 0.3811909591574647 1105 14-15 0.41851587788352873 1105 16-17 0.30514636291278663 1105 18-19 0.26613244090431465 1105 20-21 0.32085865905722244 1105 22-23 0.41433336999015324 1105 24-25 0.2661717076619965 1105 26-27 0.22144166586704017 1105 28-29 0.12147410781517465 1105 30-31 -0.015420863959590747 1105 32-33 0.1361066233809609 1105 34-35 0.04294876639689704 1105 36-37 0.2793634528494735 1105 38-39 0.3846126164984369 1105 40-41 0.20621226843277185 1105 42-43 0.2693186160507963 1105 44-45 0.2793844137445447 1105 46-47 0.33818736924482806 1105 48-49 0.35682090995205584 1105 50-51 0.26097462169971664 1105 52-53 0.21225709574270724 1105 54-55 0.11156283937700096 1105 56-57 0.3061870209823283 1105 58-59 0.3256783056125556 1105 60-61 0.08305751415367979 1105 62-63 0.14738884569118937 1105 64-65 0.3972261706886471 1105 66-67 0.504199524604168 1105 68-69 0.5973458929630517 1105 70-71 0.5377452322475129 1105 72-73 0.494528049404078 1105 74-75 0.3043330057045175 1105 76-77 0.5811203116707837 1105 78-79 0.6245096976664257 1105 80-81 0.7771870281414941 1105 82-83 0.5663188160255466 1105 84-85 0.7594060305268542 1105 86-87 0.5714276227979909 1105 88-89 0.47702781560873575 1105 90-91 0.6420986385691485 1105 92-93 0.46072805942526784 1105 94-95 0.31002773396341965 1105 96-97 0.2453622328682563 1105 98-99 0.46055941958510616 1105 100-101 0.6547709116426326 1106 1 0.09858614656966225 1106 2 0.23222468934443796 1106 3 0.3228201651610867 1106 4 0.20951405428647973 1106 5 0.2969145099989987 1106 6 0.32966790462698725 1106 7 0.3298624959260934 1106 8 0.25494542231035666 1106 9 0.38195781198707124 1106 10-11 0.3611437354087954 1106 12-13 0.3806417938364888 1106 14-15 0.4005214592497026 1106 16-17 0.44089253965953645 1106 18-19 0.33139993362711806 1106 20-21 0.49332008683479245 1106 22-23 0.23120437319020937 1106 24-25 0.3877928820134926 1106 26-27 0.32512460106640617 1106 28-29 0.5003367738059481 1106 30-31 0.5389208083250168 1106 32-33 0.42308665832547376 1106 34-35 0.23012442787762666 1106 36-37 0.3256496842601635 1106 38-39 0.40719981663500704 1106 40-41 0.3265273787100469 1106 42-43 0.43276345476778033 1106 44-45 0.7144944993986613 1106 46-47 0.8569966081130147 1106 48-49 0.7619969128646815 1106 50-51 0.6344884784342 1106 52-53 0.6917568109257957 1106 54-55 0.500394532617868 1106 56-57 0.727759158049814 1106 58-59 0.6491466961618642 1106 60-61 0.766173881457739 1106 62-63 0.7180979091400346 1106 64-65 0.689511029403775 1106 66-67 0.5725390333254907 1106 68-69 0.367363208437407 1106 70-71 0.5027863521441098 1106 72-73 0.5133224812770933 1106 74-75 0.870161422244454 1106 76-77 0.7914523716025279 1106 78-79 0.743132455495406 1106 80-81 0.7538490262496822 1106 82-83 0.9109938721614768 1106 84-85 0.8298189540722447 1106 86-87 0.7480032177343467 1106 88-89 0.7634512817346888 1106 90-91 1.0084868466265924 1106 92-93 0.7040004576184664 1106 94-95 0.5819555873149405 1106 96-97 0.7173635114958401 1106 98-99 0.5744417614594646 1106 100-101 0.5404304239176341 1107 1 0.20128307017941083 1107 2 0.2570907968499476 1107 3 0.15675582649213382 1107 4 0.13974188485161676 1107 5 0.1311412965289378 1107 6 0.19071083555502355 1107 7 0.059562787553232965 1107 8 0.27590894633069496 1107 9 0.3101829019598199 1107 10-11 0.21603915621018643 1107 12-13 0.2638591790302627 1107 14-15 0.4322904965789789 1107 16-17 0.41050509217500775 1107 18-19 0.1758863737180718 1107 20-21 0.4270288712866943 1107 22-23 0.28047103885299407 1107 24-25 0.3476326627661024 1107 26-27 0.1528236579211466 1107 28-29 0.23271115077292848 1107 30-31 0.28789810557890405 1107 32-33 0.2934343019681549 1107 34-35 0.2343545272115506 1107 36-37 0.26108890685549113 1107 38-39 0.26284814743355156 1107 40-41 0.36813694970192756 1107 42-43 0.4142870434379944 1107 44-45 0.2657388086539285 1107 46-47 0.1542429652834798 1107 48-49 0.14379255301385996 1107 50-51 0.354374588248632 1107 52-53 0.40052996918245753 1107 54-55 0.47807326090268987 1107 56-57 0.4072315689178154 1107 58-59 0.4226814078224379 1107 60-61 0.6367586917495132 1107 62-63 0.5157495828589589 1107 64-65 0.341649042825658 1107 66-67 0.46888089461939586 1107 68-69 0.33435186961285623 1107 70-71 0.4320818291627866 1107 72-73 0.64010750539531 1107 74-75 0.6633244975304358 1107 76-77 0.539961609808195 1107 78-79 0.5812279445606414 1107 80-81 0.4859272586177852 1107 82-83 0.5212989512923194 1107 84-85 0.2703553773482028 1107 86-87 0.26581330371666567 1107 88-89 0.48659247243675097 1107 90-91 0.46627494763699673 1107 92-93 0.4079123269935998 1107 94-95 0.43100215228855987 1107 96-97 0.2893680590722205 1107 98-99 0.5630075683029112 1107 100-101 0.6977584969197856 1108 1 -0.08112092576130081 1108 2 0.12856962456944387 1108 3 0.1189480673086436 1108 4 0.23107501780616957 1108 5 0.36816030665776367 1108 6 0.16442970412593638 1108 7 0.08707851082367313 1108 8 0.207367283703924 1108 9 0.16392497568627817 1108 10-11 -0.05719589456321472 1108 12-13 0.0584640611622973 1108 14-15 0.23065288483549296 1108 16-17 0.17826325761031114 1108 18-19 0.33648915833673243 1108 20-21 0.3890445687018129 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-1.7037938379307924 2316 74-75 -1.6218816581025486 2316 76-77 -1.7692280117376171 2316 78-79 -1.925098343432694 2316 80-81 -2.1312426074878843 2316 82-83 -1.83264172760234 2316 84-85 -1.7488248211923256 2316 86-87 -2.281638649913379 2316 88-89 -2.296366132081907 2316 90-91 -2.047958173130869 2316 92-93 -2.6268404834725096 2316 94-95 -2.2843409108455752 2316 96-97 -2.1583972642683413 2316 98-99 -2.2907249279169264 2316 100-101 -2.185037396403029 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1547.0 3 522.0 4 508.0 5 638.0 6 739.0 7 943.0 8 1219.0 9 1608.0 10 2139.0 11 3133.0 12 4826.0 13 6428.0 14 7573.0 15 10161.0 16 13660.0 17 19082.0 18 15720.0 19 7716.0 20 7033.0 21 7219.0 22 7460.0 23 8217.0 24 9225.0 25 10441.0 26 12125.0 27 14203.0 28 16880.0 29 19877.0 30 24568.0 31 30388.0 32 38256.0 33 49570.0 34 67225.0 35 96948.0 36 134801.0 37 183848.0 38 160906.0 39 27463.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.70702141365242 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19.051239359698315 27.830793584490976 33.893057282445966 19.224909773364747 20-21 21.405212333546405 25.731322308807098 33.29398790847919 19.569477449167305 22-23 20.465014835448745 26.179431593235954 33.49410558662843 19.86144798468687 24-25 20.437975791503852 26.35509860163709 33.77182304349459 19.435102563364463 26-27 20.16590206196884 25.866455768150843 34.639495756090206 19.32814641379011 28-29 19.287635916896875 26.87702661692683 34.372594040392315 19.462743425783984 30-31 19.548467929064756 26.572175460714153 34.02551857786035 19.85383803236074 32-33 19.566937739188813 26.701228901131714 33.80707598812667 19.924757371552804 34-35 20.383500931625655 26.220615004661784 33.62751464523334 19.768369418479217 36-37 20.41130069416933 25.801895353534565 34.068550440954624 19.718253511341484 38-39 20.07967252785855 26.645069378037118 33.64220058156555 19.633057512538787 40-41 19.62786672013942 26.916261473954968 33.725548168899735 19.730323637005878 42-43 19.458051169961553 27.377685837512928 32.37441697078511 20.789846021740402 44-45 19.97608361659432 26.869437135576423 32.187880706914044 20.966598540915214 46-47 20.77653485190599 27.413541083232012 31.441216133143122 20.368707931718873 48-49 20.592827214198035 27.67901227339879 30.758583475309763 20.969577037093416 50-51 20.172098682791788 28.160680081067973 31.08544045338738 20.58178078275286 52-53 20.422815920266935 28.891526751784095 29.817592268687314 20.868065059261657 54-55 19.844223567089056 28.136459530719105 30.341324470483077 21.67799243170876 56-57 20.784206772122687 28.415505567324573 29.331325824365084 21.46896183618766 58-59 21.31931376246517 27.751070812949386 29.75613421278477 21.17348121180068 60-61 20.864658994492018 28.08773153015924 30.639485043455434 20.40812443189331 62-63 20.84023511009259 28.09411429488684 30.58182770920861 20.483822885811964 64-65 20.512067400040888 28.748646695742075 30.353778981690688 20.38550692252635 66-67 20.63047567464703 28.377797458286675 29.54960098174836 21.442125885317935 68-69 20.4613103951719 28.47371050916004 29.98312624186566 21.081852853802403 70-71 21.11777750448493 28.599315596627566 29.47726886745492 20.805638031432583 72-73 20.7169026510671 28.08804814159845 30.061562845241856 21.13348636209259 74-75 20.18456517485186 29.730745263264737 29.24666548574624 20.83802407613716 76-77 20.4270942684976 28.652143548417797 29.522857147040348 21.397905036044254 78-79 20.359783743267595 28.67944121477764 29.769215866830045 21.191559175124716 80-81 20.52650640988094 29.270829672496674 29.099697761325167 21.102966156297224 82-83 20.356531033573948 28.83713779521794 29.494072458038666 21.31225871316944 84-85 20.14228578567051 28.8721370097245 28.990179850638476 21.99539735396651 86-87 20.318033788406233 29.107001174035375 28.996868263081517 21.578096774476883 88-89 20.438379693222046 28.439736210972665 28.69449805183557 22.42738604396972 90-91 20.11498459844696 28.030094517515607 29.45068770179158 22.404233182245857 92-93 20.482744450566692 28.862372882679445 29.297903897182664 21.3569787695712 94-95 19.967600115156216 28.43782784145526 29.361956484612495 22.232615558776025 96-97 19.37109643581624 28.47770085528894 29.671650443650332 22.479552265244486 98-99 19.811154089790627 28.822133662066552 28.645468840132 22.72124340801082 100-101 19.689813361863422 28.295598807319884 29.278354339620982 22.736233491195712 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1554.0 1 1120.0 2 795.5 3 925.5 4 812.5 5 822.5 6 1429.5 7 3052.5 8 5064.5 9 5698.0 10 4540.0 11 3470.5 12 3444.5 13 3547.0 14 3548.5 15 3562.5 16 3594.0 17 3650.0 18 3704.5 19 3799.0 20 4027.5 21 4489.0 22 5034.5 23 5644.0 24 6154.5 25 6866.5 26 8256.0 27 9820.5 28 10993.0 29 12367.0 30 14280.0 31 16309.5 32 19151.0 33 22345.5 34 25521.5 35 27557.5 36 30598.5 37 34332.5 38 34442.0 39 33073.5 40 32026.0 41 31329.5 42 30011.0 43 29632.0 44 31312.0 45 32228.0 46 31927.0 47 37108.0 48 55578.0 49 54400.5 50 34542.0 51 26264.5 52 22536.0 53 21499.5 54 21046.5 55 19609.0 56 18049.0 57 16550.0 58 15685.5 59 14839.0 60 13512.0 61 12112.0 62 10188.5 63 7284.0 64 4777.0 65 3524.0 66 2473.0 67 1692.5 68 1237.0 69 857.5 70 590.5 71 454.0 72 332.5 73 299.5 74 227.0 75 185.5 76 137.5 77 55.0 78 30.0 79 15.0 80 7.5 81 6.5 82 6.0 83 4.5 84 4.0 85 3.0 86 1.5 87 1.5 88 1.5 89 1.0 90 1.5 91 1.0 92 1.0 93 0.5 94 0.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023613856845383883 2 0.010928727135053697 3 0.012099662185238022 4 0.02166229842841001 5 0.05513152527951195 6 0.041860928044089606 7 0.01590520109833708 8 0.048593804582649475 9 0.026931506154239466 10-11 0.02254049971604825 12-13 0.03576230799104625 14-15 0.019125272486343967 16-17 0.01756402575276487 18-19 0.01756402575276487 20-21 0.013368175156271039 22-23 0.007855022628319846 24-25 0.0016100356940034464 26-27 4.878896042434686E-5 28-29 0.002244292179519956 30-31 0.0 32-33 3.9031168339477486E-4 34-35 4.878896042434686E-5 36-37 0.0 38-39 1.9515584169738743E-4 40-41 9.757792084869372E-5 42-43 1.9515584169738743E-4 44-45 1.463668812730406E-4 46-47 0.0 48-49 3.9031168339477486E-4 50-51 2.927337625460812E-4 52-53 2.439448021217343E-4 54-55 0.0019515584169738744 56-57 2.927337625460812E-4 58-59 0.0016100356940034464 60-61 0.003561594110977321 62-63 0.00248823698164169 64-65 0.0015124577731547527 66-67 0.0116605615414189 68-69 0.015026999810698834 70-71 0.027321817837634245 72-73 0.017368869911067484 74-75 0.019271639367617013 76-77 0.03098098986946026 78-79 0.020149840655255255 80-81 0.04547131111549127 82-83 0.0358110969514706 84-85 0.018930116644646583 86-87 0.014734266048152754 88-89 0.013612119958392774 90-91 0.02439448021217343 92-93 0.031761613236249805 94-95 0.012392395947784102 96-97 0.019271639367617013 98-99 0.027614551600180323 100-101 0.039665424824994 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1024822.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.97549158783271 #Duplication Level Percentage of deduplicated Percentage of total 1 73.24718949596287 30.74586786520178 2 13.563208217006256 11.38644664833939 3 5.049809856860641 6.359047535004257 4 2.485663401916247 4.173477728692763 5 1.376073971216757 2.8880690701522265 6 0.8450843210453757 2.128369788542969 7 0.5768296143197204 1.6948894636443144 8 0.4177856029492317 1.4029404849690472 9 0.2969479174074591 1.1218081328245118 >10 1.8445193124127592 14.896437452043834 >50 0.1776675066981656 5.1272246315602725 >100 0.10706493776772398 7.941414314808501 >500 0.005142857261725003 1.5301538905319312 >1k 0.006077922218402276 4.957741780115802 >5k 7.012987175079549E-4 2.3210375866770745 >10k+ 2.337662391693183E-4 1.325073626891317 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13504 1.31769224314076 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9560 0.932844923313512 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7270 0.7093914845700034 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6824 0.665871731871486 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4909 0.47901001344623745 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3753 0.36620993694514753 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3217 0.3139081713702477 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2712 0.2646313213416574 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2518 0.24570120469701082 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2438 0.2378949710291153 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2120 0.20686519219923072 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2091 0.20403543249461858 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2077 0.2026693416027369 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2067 0.20169356239424993 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2009 0.1960340429850257 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1980 0.19320428328041356 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1853 0.18081188733262948 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1761 0.17183471861454963 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1680 0.16393090702580546 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1652 0.16119872524204204 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1597 0.1558319395953639 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1286 0.12548520621142012 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1275 0.1244118490820845 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1143 0.11153156353005693 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1105 0.10782360253780657 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1102 0.1075308687752605 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1094 0.10675024540847095 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1042 0.10167619352433885 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1035 0.100993148078398 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 9.757792084869373E-5 0.0 20-21 0.0 0.0 0.0 1.9515584169738746E-4 0.0 22-23 0.0 0.0 0.0 5.854675250921623E-4 0.0 24-25 0.0 0.0 0.0 0.0012197240106086714 0.0 26-27 0.0 0.0 0.0 0.0023906590607929963 0.0 28-29 0.0 0.0 0.0 0.004732529161161646 0.0 30-31 0.0 0.0 0.0 0.00839170119298766 0.0 32-33 0.0 0.0 0.0 0.013612119958392774 0.0 34-35 0.0 0.0 0.0 0.01868617184252485 0.0 36-37 0.0 0.0 0.0 0.023516278924535186 0.0 38-39 0.0 0.0 0.0 0.027565762639755978 0.0 40-41 0.0 0.0 0.0 0.03190798011752285 0.0 42-43 0.0 0.0 0.0 0.03756749952674708 0.0 44-45 0.0 0.0 0.0 0.04356854165894175 0.0 46-47 0.0 0.0 0.0 0.050886885722593776 0.0 48-49 0.0 0.0 0.0 0.05981526548024925 0.0 50-51 0.0 0.0 0.0 0.0677678660294178 0.0 52-53 0.0 0.0 0.0 0.08718587227830785 0.0 54-55 0.0 0.0 0.0 0.12592430685523925 0.0 56-57 0.0 0.0 0.0 0.1990589585313352 0.0 58-59 0.0 0.0 0.0 0.27448669134737547 0.0 60-61 0.0 0.0 0.0 0.34288881386230974 0.0 62-63 0.0 0.0 0.0 0.42348817648333076 0.0 64-65 0.0 0.0 0.0 0.5198463733214158 0.0 66-67 0.0 0.0 0.0 0.677678660294178 0.0 68-69 0.0 0.0 0.0 0.875030005210661 0.0 70-71 0.0 0.0 0.0 1.032130457777058 0.0 72-73 0.0 0.0 0.0 1.2012329946078442 0.0 74-75 0.0 0.0 0.0 1.383654917634477 0.0 76-77 0.0 0.0 0.0 1.5747124866562192 0.0 78-79 0.0 0.0 0.0 1.7384970268007516 0.0 80-81 0.0 0.0 0.0 1.9199919595793222 0.0 82-83 0.0 0.0 0.0 2.09236335675854 0.0 84-85 0.0 0.0 0.0 2.416224476055354 0.0 86-87 0.0 0.0 0.0 2.827369045551325 0.0 88-89 0.0 0.0 0.0 3.288863822205222 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTCG 65 0.005837458 29.237242 7 GTACATG 9620 0.0 26.082827 1 TACATGG 9725 0.0 25.555634 2 ACATGGG 9700 0.0 25.180597 3 ATGGGAT 2785 0.0 25.082264 5 GAGTACT 8520 0.0 24.589912 12-13 CATGGGA 7170 0.0 23.925589 4 GTATGGT 180 1.941844E-7 23.761063 1 TAGACCC 120 1.7058116E-4 23.759901 5 GATATAG 450 0.0 23.233038 1 GCACCGT 145 2.3282717E-5 22.938353 6 AGTACTT 8785 0.0 22.874763 12-13 GTATAGG 440 0.0 22.681013 1 GTACTTT 9375 0.0 21.992056 14-15 TGGGATA 740 0.0 21.83129 6 CGTCACT 110 0.0028693909 21.600965 1 CATGGGG 2195 0.0 21.432623 4 CGCAGAA 160 4.983849E-5 20.783823 9 CATGGGC 810 0.0 20.533249 4 GGGATAG 255 1.1883458E-8 20.494736 7 >>END_MODULE