##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936981_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1024822 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10798167876958 31.0 31.0 34.0 31.0 34.0 2 32.19470112858623 33.0 31.0 34.0 30.0 34.0 3 32.27809219552273 34.0 31.0 34.0 30.0 34.0 4 35.97163409840928 37.0 35.0 37.0 35.0 37.0 5 35.8981325537508 37.0 35.0 37.0 35.0 37.0 6 35.976472011724965 37.0 35.0 37.0 35.0 37.0 7 35.92111117833146 37.0 35.0 37.0 35.0 37.0 8 35.90115259040107 37.0 35.0 37.0 35.0 37.0 9 37.533161856400426 39.0 37.0 39.0 35.0 39.0 10-11 37.44764846968547 39.0 37.0 39.0 34.5 39.0 12-13 37.35492651406781 39.0 37.0 39.0 34.0 39.0 14-15 38.38641344545687 40.0 38.0 41.0 34.0 41.0 16-17 38.35000126851297 40.0 38.0 41.0 33.5 41.0 18-19 38.62314089666303 40.0 38.0 41.0 34.0 41.0 20-21 38.68949437072975 40.0 38.0 41.0 34.0 41.0 22-23 38.59628257394942 40.0 38.0 41.0 34.0 41.0 24-25 38.464451387655615 40.0 38.0 41.0 34.0 41.0 26-27 38.234744667854514 40.0 37.5 41.0 34.0 41.0 28-29 38.12007987728601 40.0 37.0 41.0 33.5 41.0 30-31 37.99003875795016 40.0 37.0 41.0 33.5 41.0 32-33 37.67731079153258 40.0 36.0 41.0 33.0 41.0 34-35 37.449798599171366 40.0 36.0 41.0 33.0 41.0 36-37 37.19774068082067 39.5 35.0 41.0 33.0 41.0 38-39 36.976083651599986 39.0 35.0 41.0 32.0 41.0 40-41 36.7453557788572 39.0 35.0 41.0 31.0 41.0 42-43 36.37098003360583 39.0 35.0 41.0 30.5 41.0 44-45 35.8983291732613 39.0 35.0 41.0 29.5 41.0 46-47 35.25281463512688 39.0 35.0 40.5 26.0 41.0 48-49 34.655410402977296 39.0 35.0 40.0 23.0 41.0 50-51 34.28875502282348 38.0 34.0 40.0 21.0 41.0 52-53 34.0396883556364 38.0 34.0 40.0 20.0 41.0 54-55 33.76733179030114 38.0 34.0 40.0 18.0 41.0 56-57 33.48145873136993 38.0 33.0 40.0 15.5 41.0 58-59 33.14324780303311 37.5 33.0 40.0 12.5 41.0 60-61 32.76419612381467 37.0 32.5 40.0 8.5 41.0 62-63 32.41854780635076 36.0 32.0 39.0 7.5 41.0 64-65 32.023405040094765 36.0 31.5 39.0 6.0 40.0 66-67 31.6182741978607 35.0 31.0 39.0 2.0 40.0 68-69 31.31697016652648 35.0 31.0 38.0 2.0 40.0 70-71 30.84108850122265 35.0 30.5 37.5 2.0 40.0 72-73 30.343073236132714 35.0 30.0 37.0 2.0 39.0 74-75 29.83611105148016 34.0 29.0 36.5 2.0 39.0 76-77 28.43185158007927 32.5 28.0 35.0 2.0 37.0 78-79 29.155327461744577 34.0 29.0 35.0 2.0 37.5 80-81 28.933586027622358 34.0 29.0 35.0 2.0 37.0 82-83 28.5995675346548 34.0 29.0 35.0 2.0 37.0 84-85 28.245945149499132 34.0 28.5 35.0 2.0 36.0 86-87 27.93112706401697 34.0 27.5 35.0 2.0 36.0 88-89 27.5077032889614 33.5 27.0 35.0 2.0 35.0 90-91 27.01475817263876 33.0 26.0 35.0 2.0 35.0 92-93 26.556979651100384 33.0 25.0 35.0 2.0 35.0 94-95 25.996280817546854 32.5 24.5 34.5 2.0 35.0 96-97 25.328471676056914 32.0 21.5 34.0 2.0 35.0 98-99 24.571277743842344 32.0 10.0 34.0 2.0 35.0 100-101 21.28594673026145 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22128253268488152 1101 2 0.22004761939936657 1101 3 0.2212004976702815 1101 4 0.2181395065373124 1101 5 0.18763590790683793 1101 6 0.11078225130182062 1101 7 0.17664544417596773 1101 8 0.15261495776083933 1101 9 0.2883206479269802 1101 10-11 0.28212758814592576 1101 12-13 0.3350121468073084 1101 14-15 0.43151395719169017 1101 16-17 0.30599322093439696 1101 18-19 0.22721786078625428 1101 20-21 0.2644856150282635 1101 22-23 0.3707251746846296 1101 24-25 0.2868585354484665 1101 26-27 0.13665236574237127 1101 28-29 0.1945300432165027 1101 30-31 0.17004657034537018 1101 32-33 0.2991163586691812 1101 34-35 0.520399338812787 1101 36-37 0.5936559284338401 1101 38-39 0.5116040471526233 1101 40-41 0.4984364669359991 1101 42-43 0.3932404874364934 1101 44-45 0.4727189217122927 1101 46-47 0.5434293733853295 1101 48-49 0.4468919111376479 1101 50-51 0.20916515998401053 1101 52-53 0.3538242471993769 1101 54-55 0.3671623050853725 1101 56-57 0.28503486209145024 1101 58-59 0.420516290448937 1101 60-61 0.7301356782549959 1101 62-63 0.5874711594982003 1101 64-65 0.4429867716787328 1101 66-67 0.40280543817911507 1101 68-69 0.32685830889440837 1101 70-71 0.570403456889732 1101 72-73 0.6043551672496363 1101 74-75 0.5498685068510909 1101 76-77 0.5697075827115512 1101 78-79 0.5707311724897011 1101 80-81 0.7366329501508382 1101 82-83 0.639038415824448 1101 84-85 0.30544449786635397 1101 86-87 -0.03746181732515552 1101 88-89 0.09084278118575995 1101 90-91 0.6715944900315378 1101 92-93 0.6691782099852084 1101 94-95 0.25297511091363134 1101 96-97 0.09675144589779094 1101 98-99 0.23283016866037443 1101 100-101 0.6640056158152703 1102 1 0.24376268949239943 1102 2 0.30342500607514467 1102 3 0.255047855956839 1102 4 0.12454238402808926 1102 5 0.16214605232823942 1102 6 0.07093276502295964 1102 7 0.175287865673873 1102 8 0.11470062048461216 1102 9 0.3715464463318483 1102 10-11 0.386279283871815 1102 12-13 0.30707001515001764 1102 14-15 0.4275913708271446 1102 16-17 0.3643911631715895 1102 18-19 0.32649762461556264 1102 20-21 0.3720422903328995 1102 22-23 0.3265971574304203 1102 24-25 0.3700824150144584 1102 26-27 0.2737788277776687 1102 28-29 0.1996768487815217 1102 30-31 0.29754477862957174 1102 32-33 0.24667928938854544 1102 34-35 0.3277210522975267 1102 36-37 0.1516418000232278 1102 38-39 0.35764840878851345 1102 40-41 0.3555275839592724 1102 42-43 0.40770997166220013 1102 44-45 0.2543852797657067 1102 46-47 0.28963367024120856 1102 48-49 0.5410373418546115 1102 50-51 0.6297727219361207 1102 52-53 0.3343451543880178 1102 54-55 0.5564663134438348 1102 56-57 0.5840201800958198 1102 58-59 0.5617380343472647 1102 60-61 0.6057517454604096 1102 62-63 0.6960078817568274 1102 64-65 0.5185050740436097 1102 66-67 0.6722013013543915 1102 68-69 0.745751868023909 1102 70-71 0.44333266996431675 1102 72-73 0.6062903162522737 1102 74-75 0.49808624133560286 1102 76-77 0.5605336140634591 1102 78-79 0.6093079888865631 1102 80-81 0.6671091004227883 1102 82-83 0.6616773565486369 1102 84-85 0.6493234498349381 1102 86-87 0.5112718929320934 1102 88-89 0.7897217955166091 1102 90-91 0.8487867874285406 1102 92-93 0.7907544018000188 1102 94-95 0.5145742520413528 1102 96-97 0.5258513089886385 1102 98-99 0.8526338330669709 1102 100-101 1.0514105493418953 1103 1 0.36056750268372184 1103 2 0.3853430245022551 1103 3 0.2944046479486033 1103 4 0.18179298111765974 1103 5 0.24750338038808906 1103 6 0.23562724766660637 1103 7 0.2325882010388014 1103 8 0.24391881769318502 1103 9 0.31301278595400106 1103 10-11 0.43011067848699724 1103 12-13 0.4035065440447312 1103 14-15 0.549291259284459 1103 16-17 0.46876094698509974 1103 18-19 0.4334243242138811 1103 20-21 0.42760224660005974 1103 22-23 0.43076568544459093 1103 24-25 0.38052929499692567 1103 26-27 0.5976565158320994 1103 28-29 0.6416557689635596 1103 30-31 0.5475298110930069 1103 32-33 0.5255263377442816 1103 34-35 0.5719557874826577 1103 36-37 0.5388975643669625 1103 38-39 0.5171337281681048 1103 40-41 0.7579811546608468 1103 42-43 0.6525480137057755 1103 44-45 0.6308372459473688 1103 46-47 0.346070493881264 1103 48-49 0.4483823035054755 1103 50-51 0.7123557595262824 1103 52-53 0.6816422015002956 1103 54-55 0.7186982909179207 1103 56-57 0.7387605601146632 1103 58-59 0.7697490283482793 1103 60-61 0.8960680716808724 1103 62-63 0.9086721677408605 1103 64-65 0.9326568107844437 1103 66-67 0.8991188270041448 1103 68-69 0.753718281792942 1103 70-71 0.6778371247637374 1103 72-73 0.8966941449941821 1103 74-75 0.9407016272263249 1103 76-77 0.8669632993399183 1103 78-79 1.0796391076385383 1103 80-81 1.0408130268911933 1103 82-83 0.8350903141062282 1103 84-85 0.9459270624916734 1103 86-87 0.7778077970588022 1103 88-89 0.8166025701073529 1103 90-91 0.845304647614249 1103 92-93 0.7561471545648679 1103 94-95 0.7966533672158569 1103 96-97 0.8703768288687499 1103 98-99 0.8259082603169112 1103 100-101 1.260181894200386 1104 1 0.15843523699775375 1104 2 0.1785994915017568 1104 3 0.21503222931395527 1104 4 0.0959303997344989 1104 5 0.029648617490622087 1104 6 -0.062739387284239 1104 7 -0.03581321441357943 1104 8 0.12277861592351513 1104 9 0.17199584827738335 1104 10-11 0.2776306077856532 1104 12-13 0.1295272412940065 1104 14-15 0.16038529492735165 1104 16-17 0.21279641169321906 1104 18-19 0.2255716281904725 1104 20-21 0.12705024868118642 1104 22-23 0.09287052941341045 1104 24-25 0.15406537798548214 1104 26-27 0.07274578341033333 1104 28-29 -0.011611406581749861 1104 30-31 0.32913026651802113 1104 32-33 0.3230363781195962 1104 34-35 0.32926468181194224 1104 36-37 0.3963465072321455 1104 38-39 0.3399188766088628 1104 40-41 0.2661733703854807 1104 42-43 0.2245986200482335 1104 44-45 0.16539680577445637 1104 46-47 0.49534156441512067 1104 48-49 0.6257067350260215 1104 50-51 0.7482088974331091 1104 52-53 0.802396646170429 1104 54-55 0.6078189607276627 1104 56-57 0.5557807335182687 1104 58-59 0.6637295248969579 1104 60-61 0.7299083236969608 1104 62-63 0.762115651207651 1104 64-65 0.6173435902739257 1104 66-67 0.5160767269175466 1104 68-69 0.43936633590227814 1104 70-71 0.4950256771322721 1104 72-73 0.4980404639983753 1104 74-75 0.6672813604570287 1104 76-77 0.5203503001274647 1104 78-79 0.7262259145675571 1104 80-81 0.9626094611770704 1104 82-83 0.8697763808805199 1104 84-85 0.7713617296714936 1104 86-87 1.0086201676564492 1104 88-89 0.7614139143951171 1104 90-91 0.7870103354378628 1104 92-93 0.9064676982240201 1104 94-95 1.1039302784507434 1104 96-97 1.1008029969198638 1104 98-99 1.2994919170447652 1104 100-101 1.3760128132109912 1105 1 0.1376108815673902 1105 2 0.31482975325361195 1105 3 0.20521434498088098 1105 4 0.18753654219673876 1105 5 0.2439244466984931 1105 6 0.21516831461785557 1105 7 0.22996074213511974 1105 8 0.1845010419368336 1105 9 0.20874218616886253 1105 10-11 0.1413153844154209 1105 12-13 0.2576870251936967 1105 14-15 0.23284222726987025 1105 16-17 0.2543680439723346 1105 18-19 0.22196995628596738 1105 20-21 0.24649226982174355 1105 22-23 0.3218845717295551 1105 24-25 0.14085822579382068 1105 26-27 0.3058563771606586 1105 28-29 0.2090685140973818 1105 30-31 0.3901252634173531 1105 32-33 0.3048051038354629 1105 34-35 0.3851405222312465 1105 36-37 0.41124106723606957 1105 38-39 0.32232393856953223 1105 40-41 0.25679737405722847 1105 42-43 0.43404258567118603 1105 44-45 0.514723944792685 1105 46-47 0.46983030568684825 1105 48-49 0.4523929165881242 1105 50-51 0.69346733841369 1105 52-53 0.5106953817313382 1105 54-55 0.5723798930982156 1105 56-57 0.6067182893564436 1105 58-59 0.895450952392288 1105 60-61 0.749572996831219 1105 62-63 0.8597312953568519 1105 64-65 0.6284648033506528 1105 66-67 0.6922954444000169 1105 68-69 0.8423331605800328 1105 70-71 0.45636200952314354 1105 72-73 0.5215651974641418 1105 74-75 0.664014717614652 1105 76-77 0.7159266902572057 1105 78-79 0.8063482242622868 1105 80-81 0.6512303852091215 1105 82-83 0.81650807384149 1105 84-85 0.9065365403910199 1105 86-87 0.7000075996433601 1105 88-89 0.6161983045809123 1105 90-91 0.7689743852866826 1105 92-93 0.7736875788923605 1105 94-95 0.7909356178943021 1105 96-97 0.8581175791364366 1105 98-99 0.9553680202319619 1105 100-101 1.2860572910561565 1106 1 0.16814900261196897 1106 2 0.046179471833603714 1106 3 0.11915521803615547 1106 4 0.1476639171952101 1106 5 0.04116180979911377 1106 6 0.1164971117846676 1106 7 0.14824093577578168 1106 8 0.05656599064366219 1106 9 0.09349585873916766 1106 10-11 0.0836586872115106 1106 12-13 0.1458371611567486 1106 14-15 0.28211598770662505 1106 16-17 0.14914692327441514 1106 18-19 -0.02312587981278824 1106 20-21 0.18859691869562312 1106 22-23 0.1849525288694025 1106 24-25 0.13152285083715043 1106 26-27 0.2186222715989814 1106 28-29 0.3035105778092628 1106 30-31 0.2380892144811071 1106 32-33 0.15968871214340652 1106 34-35 0.013052056532089296 1106 36-37 -0.08965562697159868 1106 38-39 0.04163774757323324 1106 40-41 -0.039820708245279945 1106 42-43 -0.3136941215666482 1106 44-45 -0.48489717033083224 1106 46-47 -0.5108308876176011 1106 48-49 -0.24406498886659733 1106 50-51 -0.4013370611800795 1106 52-53 -0.27206509508874177 1106 54-55 -0.47905830163293217 1106 56-57 -0.46403911457664293 1106 58-59 -0.4328615030778522 1106 60-61 -0.5390588890747807 1106 62-63 -0.1865745335474429 1106 64-65 -0.5974315860071187 1106 66-67 -0.34354445191617344 1106 68-69 -0.3552060910221684 1106 70-71 -0.4984461702093945 1106 72-73 -0.4676135020636032 1106 74-75 -0.37597026635024733 1106 76-77 -0.3377251502522931 1106 78-79 -0.23242911859629345 1106 80-81 -0.267544536270254 1106 82-83 -0.18484458871754228 1106 84-85 -0.19557380482100584 1106 86-87 -0.23341056906231472 1106 88-89 -0.4372945784804081 1106 90-91 -0.19034775047429875 1106 92-93 -0.3394835917138366 1106 94-95 -0.27926129772150077 1106 96-97 -0.26100731002884814 1106 98-99 -0.29800405516873596 1106 100-101 -0.5071107613710595 1107 1 0.06970602048605201 1107 2 0.07469719282523357 1107 3 0.13390974428008207 1107 4 0.08309435584057923 1107 5 -0.0038295819299136724 1107 6 0.04844659654325767 1107 7 0.08283564432431945 1107 8 7.276755558507375E-4 1107 9 -0.00798386037521226 1107 10-11 0.07058315026119999 1107 12-13 0.05551682304996319 1107 14-15 0.007232312301184152 1107 16-17 0.03202675381947273 1107 18-19 0.036210957061733495 1107 20-21 0.05765081199322708 1107 22-23 0.04949272673415095 1107 24-25 0.06122615410428267 1107 26-27 -0.03571323513454416 1107 28-29 0.07132442834157615 1107 30-31 -0.058413016642184346 1107 32-33 0.12311234448240072 1107 34-35 0.05829190856017874 1107 36-37 0.15286295735682387 1107 38-39 0.0035266983680557473 1107 40-41 0.11609942306222365 1107 42-43 0.21947909290729228 1107 44-45 0.2039251278516474 1107 46-47 0.31589942504081847 1107 48-49 -0.04523777167214149 1107 50-51 0.0695541834805411 1107 52-53 0.0752723641913704 1107 54-55 0.11653742585379945 1107 56-57 0.318000970351612 1107 58-59 0.21760128560038794 1107 60-61 0.1840584594773702 1107 62-63 0.34055281364946666 1107 64-65 0.07824741332530039 1107 66-67 0.06279856383972415 1107 68-69 0.09185429737900108 1107 70-71 -0.07273328992710049 1107 72-73 0.02558521096224453 1107 74-75 -0.11137259635571795 1107 76-77 -0.043259540671261476 1107 78-79 -0.16345628860839412 1107 80-81 0.019311662939692553 1107 82-83 0.008166398749015258 1107 84-85 -0.09873326613899636 1107 86-87 -0.18482779664693183 1107 88-89 -0.1955464720490525 1107 90-91 -0.044633314178014416 1107 92-93 -0.03143674063441537 1107 94-95 -0.07855682493417859 1107 96-97 0.1215738662173429 1107 98-99 0.17902466081855195 1107 100-101 0.12506007892669402 1108 1 0.15439352654588845 1108 2 -0.014092422382866232 1108 3 0.1603613592684212 1108 4 0.15059453359594954 1108 5 0.16964673135907304 1108 6 0.15098962950233386 1108 7 0.17636648003310995 1108 8 0.14362036948696044 1108 9 0.08692457944975018 1108 10-11 0.15467840774793018 1108 12-13 0.1336851509213588 1108 14-15 0.10920208419256028 1108 16-17 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-0.7775195104128443 2316 62-63 -1.3400127858070618 2316 64-65 -1.2765999559031087 2316 66-67 -1.2788785119606842 2316 68-69 -1.3775127177197177 2316 70-71 -0.9649919075941398 2316 72-73 -0.7269869407700575 2316 74-75 -0.9004712201586678 2316 76-77 -0.8236309276061569 2316 78-79 -0.8765819323311383 2316 80-81 -0.8518260005932774 2316 82-83 -0.5267877462776092 2316 84-85 -0.7044078875752398 2316 86-87 -0.8550239814254859 2316 88-89 -1.0894185054459378 2316 90-91 -1.1595789127579863 2316 92-93 -1.0253172656930367 2316 94-95 -0.7078903116652704 2316 96-97 -0.5331606460873495 2316 98-99 -0.9541163774651444 2316 100-101 -1.1588997199031041 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 9.0 5 16.0 6 77.0 7 187.0 8 396.0 9 784.0 10 1474.0 11 2650.0 12 4051.0 13 5838.0 14 7262.0 15 10461.0 16 14504.0 17 20632.0 18 17291.0 19 8711.0 20 6712.0 21 6573.0 22 6690.0 23 7245.0 24 8436.0 25 9597.0 26 11367.0 27 14018.0 28 17244.0 29 21184.0 30 26301.0 31 32483.0 32 40991.0 33 52956.0 34 72283.0 35 101256.0 36 146718.0 37 204786.0 38 135518.0 39 8119.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.1500214734705 20.46919533049623 15.905790028501151 18.474993167532112 2 15.283414538467618 23.552481481228487 39.37035688949043 21.793747090813458 3 19.71259258970138 29.20862944311033 31.57549103888792 19.50328692830037 4 11.767042017134715 18.33073125036592 39.42145937823228 30.48076735426709 5 11.354470683172964 40.847523616319336 33.207262536579556 14.59074316392814 6 26.925749056909392 39.26945362219 17.76493869179233 16.039858629108274 7 24.99751176301836 37.00457250137097 21.359026250412267 16.638889485198405 8 26.539633224111114 32.214082055225205 20.18311472626466 21.06316999439903 9 27.63601874276704 16.995244052137835 21.893362944979714 33.475374260115416 10-11 24.0562739490476 28.126874021361342 26.681770252117325 21.135081777473733 12-13 27.49789719580571 26.651555099324565 27.386804732919472 18.46374297195025 14-15 23.579119105561748 26.40804939784665 29.532396845500976 20.48043465109063 16-17 19.184502284299125 29.99530650200718 29.589040828553642 21.231150385140054 18-19 19.066214683973154 27.7047241558989 34.34526911119828 18.883792048929664 20-21 21.334801570825466 25.52747505272921 34.05114013650315 19.086583239942176 22-23 20.440896429883534 25.969105146036025 34.15500705748496 19.434991366595483 24-25 20.514739751266696 26.162424173816646 34.45773056234337 18.865105512573287 26-27 20.31988735000249 25.615578955996828 35.35428053401407 18.71025315998661 28-29 19.28006693874763 26.76476543384554 34.962517716569046 18.992649910837784 30-31 19.60931546042071 26.362887652213963 34.53865227153634 19.489144615828984 32-33 19.717907845797818 26.564019393591643 34.367173560154626 19.350899200455906 34-35 20.409788236396174 25.933576757719877 34.3674804014746 19.28915460440935 36-37 20.518619357105216 25.63855221812362 34.6433241602748 19.19950426449636 38-39 20.157951750790488 26.362376546824372 34.4189893430144 19.06068235937073 40-41 19.776260310477973 26.80353471434134 34.14037960318382 19.27982537199687 42-43 19.551248899808943 27.320451746742364 32.88688181947694 20.241417533971752 44-45 20.21150990123163 26.63867481377254 32.64586435498067 20.503950930015165 46-47 21.004412490803244 27.528985634522424 31.678454125513017 19.788147749161315 48-49 20.975675920947477 27.611933016324368 30.977315286005773 20.435075776722382 50-51 20.439657166165894 28.09681942868184 31.309691213394835 20.153832191757427 52-53 20.772680524032467 28.90111648657035 29.98335320670321 20.34284978269397 54-55 20.071419649722674 28.126793053782833 30.50377248757792 21.298014808916577 56-57 21.05575676375891 28.40049317830191 29.571620877230902 20.972129180708276 58-59 21.710913418050566 27.65102393491156 29.922786515889115 20.71527613114876 60-61 20.960172442478108 28.13353363150373 30.880006010896704 20.026287915121454 62-63 21.06789048838371 27.957693838995752 30.7708671621284 20.20354851049213 64-65 20.68789540422405 28.778084768908535 30.491261161090005 20.042758665777406 66-67 20.99928570870967 28.228313648037844 29.702807984449525 21.069592658802957 68-69 20.78530041431747 28.42665722108706 30.067826677517374 20.720215687078095 70-71 21.38130835832168 28.584246265896834 29.671080813925688 20.363364561855796 72-73 21.137036480481488 27.95422034265463 30.177533269192114 20.731209907671772 74-75 20.572206685648826 29.650124607004923 29.344900870590212 20.432767836756042 76-77 20.82703142594519 28.567546364149095 29.64090349348472 20.964518716420997 78-79 20.654235291879044 28.692832110085924 29.75577173876451 20.89716085927052 80-81 20.973738367276443 29.373450311913608 29.04694853547462 20.605862785335326 82-83 20.70897643527765 28.839252123938152 29.51672630267638 20.935045138107824 84-85 20.27683362755689 28.854694549106057 29.175559244328515 21.69291257900854 86-87 20.471559241800968 29.092709050465448 28.9450662367818 21.49066547095179 88-89 20.665433929860853 28.552672664467906 28.716408735192523 22.065484670478718 90-91 20.35442442622655 28.107373368793343 29.343058001115324 22.19514420386479 92-93 20.701361163870978 29.100628743639746 29.142538481091584 21.055471611397692 94-95 20.273091460317723 28.702608210307844 29.07725882019454 21.94704150917989 96-97 19.71994330559601 28.725473693455015 29.333645591668557 22.220937409280413 98-99 20.20829389482066 29.139433763790468 28.322105148116417 22.33016719327246 100-101 19.908618098788082 28.835649187174333 28.777004742296207 22.47872797174138 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2629.0 1 1806.0 2 1055.5 3 1152.5 4 994.0 5 945.5 6 1509.5 7 3052.0 8 4861.0 9 5520.0 10 4761.5 11 3766.0 12 3522.5 13 3607.0 14 3654.5 15 3702.0 16 3750.0 17 3816.5 18 3925.0 19 4084.0 20 4305.5 21 4645.5 22 5216.0 23 5920.5 24 6381.5 25 7126.5 26 8543.0 27 9966.0 28 11046.0 29 12423.0 30 14462.5 31 16452.0 32 19149.0 33 22245.5 34 25142.0 35 27016.0 36 30054.5 37 33664.5 38 33868.0 39 32928.5 40 31919.0 41 30983.0 42 29535.5 43 29575.0 44 31470.5 45 32232.5 46 31720.0 47 37244.5 48 57203.0 49 55177.0 50 33661.0 51 25460.5 52 21966.0 53 21228.0 54 20847.5 55 19421.5 56 17793.0 57 16275.5 58 15712.5 59 15118.0 60 13746.0 61 11826.5 62 9750.0 63 7195.0 64 4809.5 65 3456.0 66 2307.5 67 1588.5 68 1173.5 69 829.0 70 569.5 71 434.5 72 344.5 73 294.0 74 217.0 75 169.0 76 129.0 77 52.5 78 12.0 79 7.5 80 6.0 81 1.5 82 1.0 83 1.0 84 1.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029468532096305507 2 0.00497647396328338 3 4.878896042434686E-4 4 1.9515584169738743E-4 5 9.757792084869372E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011758139462267593 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.9031168339477486E-4 20-21 0.0010245681689112843 22-23 0.0022930811399443024 24-25 0.01073357129335631 26-27 0.005366785646678155 28-29 4.3910064381912176E-4 30-31 0.0030249155463095054 32-33 0.004927685002859033 34-35 0.0 36-37 0.008001389509592885 38-39 0.013856064760514507 40-41 0.0014636688127304058 42-43 0.0 44-45 0.0 46-47 3.9031168339477486E-4 48-49 0.0034152272297042806 50-51 0.0067328765385598665 52-53 0.0 54-55 0.0033176493088555867 56-57 0.003951905794372096 58-59 9.757792084869372E-5 60-61 0.0016100356940034464 62-63 0.005269207725829461 64-65 4.878896042434686E-5 66-67 0.0029273376254608115 68-69 3.9031168339477486E-4 70-71 0.001073357129335631 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0021955032190956085 80-81 0.001853980496125181 82-83 0.013026652433300614 84-85 0.008879590797231128 86-87 0.0050252629237077275 88-89 1.9515584169738743E-4 90-91 5.366785646678155E-4 92-93 4.878896042434686E-5 94-95 4.878896042434686E-5 96-97 0.002878548665036465 98-99 0.0019515584169738744 100-101 1.9515584169738743E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1024822.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.98482951274876 #Duplication Level Percentage of deduplicated Percentage of total 1 71.72213111413689 28.677971848897766 2 14.16877111972842 11.330717952549985 3 5.374468591651949 6.446916310764782 4 2.65248501650942 4.2423664468099895 5 1.4899241025194394 2.9787180613087494 6 0.9278924950011909 2.2260973931269015 7 0.5976162182882591 1.672690782161678 8 0.45554612980027365 1.4571947468205166 9 0.3393404980340289 1.2211624764595708 >10 1.9506416985134316 15.065046189612193 >50 0.18770604852234163 5.156232541524322 >100 0.11924596711726247 8.382510092613561 >500 0.006870138016542152 1.910056984833091 >1k 0.006379413872503426 5.19057912047688 >5k 4.907241440387252E-4 1.634767041875484 >10k+ 4.907241440387252E-4 2.4069720101646235 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13845 1.3509663141501644 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10689 1.0430103959516872 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8946 0.8729320799124141 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7717 0.7530088151893695 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4010 0.39128746260326186 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3740 0.36494142397411455 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3294 0.3214216712755971 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2848 0.27790191857707974 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2675 0.26102093827025574 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2580 0.2517510357896298 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2530 0.24687213974719513 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2483 0.2422859774673065 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2458 0.23984652944608917 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2412 0.23535794508704927 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2381 0.23233302954073975 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2059 0.2009129390274604 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1931 0.1884229651588276 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1880 0.1834464911955442 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1845 0.18003126396583993 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1703 0.16617519920532542 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1657 0.16168661484628552 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1509 0.14724508256067884 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1238 0.12080146601068283 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1174 0.11455647907636643 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1171 0.11426374531382036 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1129 0.11016547263817522 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1057 0.10313986233706927 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1053 0.10274955065367448 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1051 0.10255439481197712 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1039 0.10138345976179278 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 9.757792084869373E-5 0.0 20-21 0.0 0.0 0.0 9.757792084869373E-5 0.0 22-23 0.0 0.0 0.0 4.3910064381912176E-4 0.0 24-25 0.0 0.0 0.0 0.001024568168911284 0.0 26-27 0.0 0.0 0.0 0.0021955032190956085 0.0 28-29 0.0 0.0 0.0 0.004537373319464258 0.0 30-31 0.0 0.0 0.0 0.008342912232563313 0.0 32-33 0.0 0.0 0.0 0.013660908918817122 0.0 34-35 0.0 0.0 0.0 0.018930116644646583 0.0 36-37 0.0 0.0 0.0 0.02380901268708127 0.0 38-39 0.0 0.0 0.0 0.027956074323150754 0.0 40-41 0.0 0.0 0.0 0.032444658682190666 0.0 42-43 0.0 0.0 0.0 0.03825054497268794 0.0 44-45 0.0 0.0 0.0 0.04400764230276087 0.0 46-47 0.0 0.0 0.0 0.051179619485139854 0.0 48-49 0.0 0.0 0.0 0.0599616323615223 0.0 50-51 0.0 0.0 0.0 0.06801181083153952 0.0 52-53 0.0 0.0 0.0 0.08767376188255131 0.0 54-55 0.0 0.0 0.0 0.1269000860637262 0.0 56-57 0.0 0.0 0.0 0.20042504942321693 0.0 58-59 0.0 0.0 0.0 0.27580399327883276 0.0 60-61 0.0 0.0 0.0 0.34435248267504015 0.0 62-63 0.0 0.0 0.0 0.42490305633563685 0.0 64-65 0.0 0.0 0.0 0.5214564090154192 0.0 66-67 0.0 0.0 0.0 0.6786056505422405 0.0 68-69 0.0 0.0 0.0 0.8769815636276348 0.0 70-71 0.0 0.0 0.0 1.033301392827242 0.0 72-73 0.0 0.0 0.0 1.2030869751039692 0.0 74-75 0.0 0.0 0.0 1.38492343060551 0.0 76-77 0.0 0.0 0.0 1.5755906879438575 0.0 78-79 0.0 0.0 0.0 1.7394728060092386 0.0 80-81 0.0 0.0 0.0 1.920967738787809 0.0 82-83 0.0 0.0 0.0 2.0943149151755134 0.0 84-85 0.0 0.0 0.0 2.41988364808718 0.0 86-87 0.0 0.0 0.0 2.8350289123379477 0.0 88-89 0.0 0.0 0.0 3.2991582928547594 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGCTTA 445 0.0 27.756102 6 GATATAG 510 0.0 25.147587 1 GAGTACT 9930 0.0 24.655773 12-13 CAGTCGA 100 0.0016523822 23.752817 9 TAGTACG 100 0.0016547306 23.747023 4 AGTACTT 10190 0.0 23.257635 12-13 GTACTTT 10735 0.0 22.652029 14-15 TAAGGTG 295 5.456968E-12 22.545048 5 GTACATG 9710 0.0 22.454128 1 TACATGG 9845 0.0 21.951088 2 CTTACTA 565 0.0 21.861 9 CTTTGCG 285 8.367351E-11 21.665009 2 ACATGGG 9655 0.0 21.250574 3 CGTATAG 180 5.1669067E-6 21.111555 1 CGTCACT 135 3.7950178E-4 21.111555 1 CATGGGG 2280 0.0 20.622416 4 ACTTTTT 11980 0.0 20.536825 16-17 TGGGATA 900 0.0 20.057936 6 CATGGGA 7180 0.0 19.976604 4 GCTTATA 335 3.45608E-11 19.851164 1 >>END_MODULE