##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936980_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1031815 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.729454408009186 33.0 31.0 34.0 30.0 34.0 2 31.760497763649493 33.0 31.0 34.0 30.0 34.0 3 31.74507930200666 33.0 31.0 34.0 30.0 34.0 4 35.39336218217413 37.0 35.0 37.0 33.0 37.0 5 35.42566448442793 37.0 35.0 37.0 33.0 37.0 6 35.532504373361505 37.0 35.0 37.0 33.0 37.0 7 35.49026618143756 37.0 35.0 37.0 33.0 37.0 8 35.46331949041252 37.0 35.0 37.0 33.0 37.0 9 37.04114884935768 39.0 37.0 39.0 33.0 39.0 10-11 36.91006963457596 39.0 37.0 39.0 33.0 39.0 12-13 36.82067037211128 39.0 37.0 39.0 32.5 39.0 14-15 37.801718331290004 40.0 37.0 41.0 32.5 41.0 16-17 37.78251769939379 40.0 37.0 41.0 32.5 41.0 18-19 37.88869467879417 40.0 37.5 41.0 33.0 41.0 20-21 37.870911936732846 40.0 37.5 41.0 33.0 41.0 22-23 37.75362250015749 40.0 37.0 41.0 32.5 41.0 24-25 37.67832508734608 40.0 37.0 41.0 32.0 41.0 26-27 37.63969025455145 40.0 37.0 41.0 32.0 41.0 28-29 37.55697726821184 40.0 37.0 41.0 32.0 41.0 30-31 37.31351792714779 39.0 36.5 41.0 32.0 41.0 32-33 37.005007680640425 39.0 35.5 41.0 31.0 41.0 34-35 36.77127149731299 39.0 35.0 41.0 31.0 41.0 36-37 36.50867015889476 39.0 35.0 41.0 30.0 41.0 38-39 36.28806084424049 39.0 35.0 41.0 30.0 41.0 40-41 36.07096184878103 39.0 35.0 40.0 30.0 41.0 42-43 35.741705150632626 38.0 35.0 40.0 29.0 41.0 44-45 35.30099387971681 38.0 35.0 40.0 26.5 41.0 46-47 34.68863943633306 38.0 34.5 40.0 23.5 41.0 48-49 34.192597025629595 38.0 34.0 40.0 21.0 41.0 50-51 33.38555748850327 37.5 33.0 39.5 18.5 40.5 52-53 33.54952050512931 38.0 33.5 39.5 18.0 40.5 54-55 33.92833841337837 38.0 34.0 40.0 18.0 41.0 56-57 33.79955369906427 38.0 34.0 40.0 15.0 41.0 58-59 33.579280685006516 38.0 34.0 40.0 13.5 41.0 60-61 33.25940405983631 38.0 33.0 40.0 10.5 41.0 62-63 32.975446179789984 37.0 33.0 40.0 8.0 41.0 64-65 32.63065326633166 36.5 32.5 39.5 7.0 41.0 66-67 32.230593178040635 36.0 32.0 39.0 3.5 41.0 68-69 31.83919888739745 35.5 32.0 39.0 2.0 41.0 70-71 31.381765142007048 35.0 31.0 38.5 2.0 40.0 72-73 30.997209771131452 35.0 31.0 37.5 2.0 40.0 74-75 30.632241729379782 35.0 31.0 37.0 2.0 39.0 76-77 30.19461386004274 35.0 30.0 36.5 2.0 39.0 78-79 29.736187204101512 35.0 30.0 36.0 2.0 39.0 80-81 29.292962401205642 34.0 29.0 36.0 2.0 37.5 82-83 28.89280588089919 34.0 29.0 35.0 2.0 37.0 84-85 28.522794783948672 34.0 29.0 35.0 2.0 36.5 86-87 28.217664019228252 34.0 29.0 35.0 2.0 36.0 88-89 27.820981958975203 34.0 27.5 35.0 2.0 36.0 90-91 27.421417114502113 34.0 26.5 35.0 2.0 35.5 92-93 27.052535095923204 34.0 25.5 35.0 2.0 35.0 94-95 26.674843843130795 34.0 25.0 35.0 2.0 35.0 96-97 26.18263545306087 33.0 24.5 35.0 2.0 35.0 98-99 25.662999665637734 33.0 21.5 35.0 2.0 35.0 100-101 23.602365249584473 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.027883275175440758 1101 2 0.0988098298565454 1101 3 0.20789153343204347 1101 4 0.1458217219742437 1101 5 0.11854395418465913 1101 6 0.10592683795845659 1101 7 0.10799037049915938 1101 8 0.02655049030452261 1101 9 0.10857090908938716 1101 10-11 0.3122791405704817 1101 12-13 0.3941027214530095 1101 14-15 0.5128477428701359 1101 16-17 0.39136521363216303 1101 18-19 0.4146539355806169 1101 20-21 0.3963704842535307 1101 22-23 0.36975520342441115 1101 24-25 0.3489459636823753 1101 26-27 0.3220303528309998 1101 28-29 0.3971997373735334 1101 30-31 0.22415159109432636 1101 32-33 0.41952257066689214 1101 34-35 0.43668607378507573 1101 36-37 0.470543496117358 1101 38-39 0.547368191710639 1101 40-41 0.438497925945569 1101 42-43 0.3449641022661609 1101 44-45 0.6674300876794135 1101 46-47 0.6823018461085439 1101 48-49 0.8772648198193167 1101 50-51 0.9995733239758522 1101 52-53 0.9586635810221367 1101 54-55 1.0362595879102798 1101 56-57 1.0271724761350498 1101 58-59 0.9842187950204035 1101 60-61 0.8327369238311988 1101 62-63 0.9758019525243427 1101 64-65 0.9235672198548386 1101 66-67 0.9411479448483746 1101 68-69 0.8582279796226473 1101 70-71 0.9937285823761677 1101 72-73 1.014928431749194 1101 74-75 1.062287661817269 1101 76-77 1.1856279532842464 1101 78-79 1.2253302925136964 1101 80-81 1.360396771040616 1101 82-83 1.2539692004964564 1101 84-85 1.0779753841403625 1101 86-87 1.434907483907402 1101 88-89 1.5351090614402274 1101 90-91 1.424866379699143 1101 92-93 1.4234789345698395 1101 94-95 1.437230334041498 1101 96-97 1.5733427917280913 1101 98-99 1.4378171638562343 1101 100-101 1.762623048353774 1102 1 0.318893289534099 1102 2 0.1694709243254806 1102 3 0.3458355392672523 1102 4 0.2867971012399835 1102 5 0.3880242442937032 1102 6 0.28066478104680215 1102 7 0.29222429270553363 1102 8 0.09457840022575681 1102 9 0.3389424450066727 1102 10-11 0.31317047812731147 1102 12-13 0.31157715800197394 1102 14-15 0.2962591389625615 1102 16-17 0.3082538354307047 1102 18-19 0.2623076947497509 1102 20-21 0.21901908256443647 1102 22-23 0.14752727240099972 1102 24-25 0.18549814339951354 1102 26-27 0.4350673687998423 1102 28-29 0.3956551498522529 1102 30-31 0.5424650685035246 1102 32-33 0.5784815911589405 1102 34-35 0.45285538663400615 1102 36-37 0.44455662534065965 1102 38-39 0.4170838169543387 1102 40-41 0.35124134642477145 1102 42-43 0.47594026139491774 1102 44-45 0.5853339076319841 1102 46-47 0.7222620272202604 1102 48-49 0.8389937302589345 1102 50-51 0.6780880977926387 1102 52-53 0.7323253131437824 1102 54-55 0.7216564235013934 1102 56-57 0.9117068749753443 1102 58-59 0.8740278203292462 1102 60-61 1.00012718104837 1102 62-63 0.7517679504966992 1102 64-65 0.8948425711372536 1102 66-67 0.8996789581656799 1102 68-69 0.944317058329176 1102 70-71 0.9583874840537909 1102 72-73 0.9933138400533608 1102 74-75 1.07025143947374 1102 76-77 1.0141227717825956 1102 78-79 1.1144094577850225 1102 80-81 1.0825389171318953 1102 82-83 1.1256587966650322 1102 84-85 1.1993243636666655 1102 86-87 1.002666111715481 1102 88-89 1.1675073237219245 1102 90-91 1.0231465523699903 1102 92-93 1.054639967108475 1102 94-95 0.8998556282104175 1102 96-97 0.8013550126403501 1102 98-99 0.9502619426733219 1102 100-101 1.0028337031175916 1103 1 0.06338300935938435 1103 2 0.013863879121121414 1103 3 0.17472211999947262 1103 4 0.1336429163743844 1103 5 0.07994303268900183 1103 6 0.1606450729398503 1103 7 0.0874073472845609 1103 8 0.1888843552699626 1103 9 0.09203715045036631 1103 10-11 0.10763178987050281 1103 12-13 0.08072109820296447 1103 14-15 0.3332491251136176 1103 16-17 0.3566341308747596 1103 18-19 0.32108889925243034 1103 20-21 0.22540424289255157 1103 22-23 0.2713434543547706 1103 24-25 0.28744720415729574 1103 26-27 0.20748578433906317 1103 28-29 0.245294722310625 1103 30-31 0.40145089997258054 1103 32-33 0.21915264332328377 1103 34-35 0.18679683047478335 1103 36-37 0.3268632728318721 1103 38-39 0.16705851600622168 1103 40-41 0.1363226645597777 1103 42-43 0.16173044995356634 1103 44-45 0.13382872315700212 1103 46-47 0.35001184079222014 1103 48-49 0.21804543296835277 1103 50-51 0.28004470444723495 1103 52-53 0.31212583514228953 1103 54-55 0.3249579752928753 1103 56-57 0.29387236980862497 1103 58-59 0.3914046890483931 1103 60-61 0.41510667396410383 1103 62-63 0.32388586179246204 1103 64-65 0.5034715260749323 1103 66-67 0.4162985739463707 1103 68-69 0.3271252860199496 1103 70-71 0.40281506119948673 1103 72-73 0.3735120752012584 1103 74-75 0.1952887250814932 1103 76-77 0.23530232861651967 1103 78-79 0.42563819611107334 1103 80-81 0.31280462147477905 1103 82-83 0.35117813191591196 1103 84-85 0.36485293154422394 1103 86-87 0.7210053042157476 1103 88-89 0.6292300963507387 1103 90-91 0.6245341096300301 1103 92-93 0.6461486138184647 1103 94-95 0.7767505360617015 1103 96-97 0.6515436600605398 1103 98-99 0.6089761218572995 1103 100-101 0.5436925149495551 1104 1 0.07711969124675377 1104 2 0.07096687252548506 1104 3 0.2089052757579708 1104 4 0.3148326677696147 1104 5 0.18274617830036277 1104 6 0.12666485985004527 1104 7 0.0012328165894786025 1104 8 0.12351144811623982 1104 9 0.14059118393591064 1104 10-11 0.32575710696842464 1104 12-13 0.2355219607093062 1104 14-15 0.22019737473310386 1104 16-17 0.39604940231058094 1104 18-19 -0.004589690296029403 1104 20-21 0.09174746968098191 1104 22-23 0.007739953593734583 1104 24-25 0.0958883507833761 1104 26-27 -0.0107917397603714 1104 28-29 0.15909482378194895 1104 30-31 0.229609195254163 1104 32-33 -0.08856552004557017 1104 34-35 -0.08077039428422239 1104 36-37 -0.18826229794438376 1104 38-39 -0.01640799845343821 1104 40-41 -0.003771196424487755 1104 42-43 -0.07394306602817835 1104 44-45 -0.060279597492417736 1104 46-47 -0.1172065762549579 1104 48-49 -0.31687338996416514 1104 50-51 -0.4125478881453546 1104 52-53 -0.31003930945436053 1104 54-55 -0.19956003586897708 1104 56-57 -0.2543201720483026 1104 58-59 -0.24068562043917296 1104 60-61 -0.38890778570104345 1104 62-63 -0.6276806019315373 1104 64-65 -0.559998793559771 1104 66-67 -0.7225015275249618 1104 68-69 -0.5155370681150053 1104 70-71 -0.6094502863398326 1104 72-73 -0.42561907649838204 1104 74-75 -0.41456667826706095 1104 76-77 -0.33674866072899334 1104 78-79 -0.29178316360480494 1104 80-81 -0.39107031510563317 1104 82-83 -0.6326118224219854 1104 84-85 -0.6810882150818536 1104 86-87 -0.23158221481012475 1104 88-89 -0.27200024357822983 1104 90-91 -0.4848646727139574 1104 92-93 -0.4811091233707785 1104 94-95 -0.35222587185306153 1104 96-97 -0.41288429529206994 1104 98-99 -0.4608107883405701 1104 100-101 -0.4218452831224866 1105 1 0.07308246291129805 1105 2 0.12063376375985868 1105 3 0.28739667980518036 1105 4 -0.01855805508888153 1105 5 0.24213186086143423 1105 6 0.26151028241161356 1105 7 0.3014024899682255 1105 8 0.15473134980548764 1105 9 0.17382221420264443 1105 10-11 0.27139924585107167 1105 12-13 0.18300555479070368 1105 14-15 0.3435861014342052 1105 16-17 0.40061561235624055 1105 18-19 0.3864425725979004 1105 20-21 0.37146526292898585 1105 22-23 0.23111121391760747 1105 24-25 0.3611190317225734 1105 26-27 0.33087679830385497 1105 28-29 0.3721010368218529 1105 30-31 0.35573140999079556 1105 32-33 0.18541706348114673 1105 34-35 -0.06163972083061964 1105 36-37 -0.0884145049459093 1105 38-39 0.09768237757270981 1105 40-41 0.14635605860956247 1105 42-43 0.2635747122486407 1105 44-45 0.012008747443580603 1105 46-47 0.28978870782319177 1105 48-49 0.3470223607157763 1105 50-51 0.13544846886739492 1105 52-53 0.2668389498963819 1105 54-55 0.13641820601539933 1105 56-57 0.36042519312374566 1105 58-59 0.1928922421752972 1105 60-61 0.39299829509342743 1105 62-63 0.34641925584225675 1105 64-65 0.5246008521222905 1105 66-67 0.5071152102548098 1105 68-69 0.42224520101691354 1105 70-71 0.3580822135977897 1105 72-73 0.5128032105989533 1105 74-75 0.6081757924767537 1105 76-77 0.7002613450099595 1105 78-79 0.717175840312656 1105 80-81 0.5688231399933024 1105 82-83 0.6774076401126337 1105 84-85 0.5162494099898858 1105 86-87 0.6402220619571004 1105 88-89 0.7243879591315512 1105 90-91 0.6571843828540764 1105 92-93 0.7251862580200452 1105 94-95 0.8020009914351043 1105 96-97 0.7068639393562286 1105 98-99 0.5444041728591209 1105 100-101 0.7354806145245405 1106 1 0.23356251292815244 1106 2 0.24425520045203797 1106 3 0.2643398537447261 1106 4 0.24173478100987467 1106 5 0.29118819443751676 1106 6 0.2557366890046211 1106 7 0.4091945707496336 1106 8 0.34577686618932546 1106 9 0.4837171334055199 1106 10-11 0.4723267580889612 1106 12-13 0.3537631599195237 1106 14-15 0.48849307951864773 1106 16-17 0.5479470281440371 1106 18-19 0.4722789315666205 1106 20-21 0.29140642676386364 1106 22-23 0.24196639033005596 1106 24-25 0.15002355087793973 1106 26-27 0.07456880695900026 1106 28-29 0.13151325148216841 1106 30-31 0.3142771490404428 1106 32-33 0.3042725786948992 1106 34-35 0.5439387998650673 1106 36-37 0.3405297797030755 1106 38-39 0.12849321292750204 1106 40-41 0.2807715477670527 1106 42-43 0.4476564646547416 1106 44-45 0.31735691817848277 1106 46-47 0.7136330722825832 1106 48-49 0.7690033179866376 1106 50-51 0.9254514726727905 1106 52-53 0.6690463102674542 1106 54-55 0.7307647648244924 1106 56-57 0.7793962325975912 1106 58-59 0.8572492968393064 1106 60-61 0.800702444710879 1106 62-63 0.8102530895187599 1106 64-65 0.5640659651928601 1106 66-67 0.5180873953660665 1106 68-69 0.6303189758747152 1106 70-71 0.8462110698639513 1106 72-73 0.8710703117695644 1106 74-75 0.927394296616594 1106 76-77 0.8683893106128977 1106 78-79 0.7788389879863651 1106 80-81 0.9205063188576865 1106 82-83 0.64051977461326 1106 84-85 0.8790942582016577 1106 86-87 1.0045836572571893 1106 88-89 1.1444967772214447 1106 90-91 1.1976431966652932 1106 92-93 1.1749659498505665 1106 94-95 1.3341796934069663 1106 96-97 1.5084499311446642 1106 98-99 1.7637806387423502 1106 100-101 1.9131884490428064 1107 1 0.2833036442800214 1107 2 0.3671443024015417 1107 3 0.24628296656031878 1107 4 0.3338281015595683 1107 5 0.1769536146995776 1107 6 0.22580380309857873 1107 7 0.38714279702000454 1107 8 0.36999546638107716 1107 9 0.3850530234662415 1107 10-11 0.44030374754245827 1107 12-13 0.3945173944992675 1107 14-15 0.48142372828822744 1107 16-17 0.5158537075943386 1107 18-19 0.5070280526915809 1107 20-21 0.6508407508290546 1107 22-23 0.552573613084931 1107 24-25 0.5850794792895755 1107 26-27 0.6227503346036158 1107 28-29 0.5669878440037408 1107 30-31 0.537482646004328 1107 32-33 0.4019171935878276 1107 34-35 0.3886751373530828 1107 36-37 0.2688870823556826 1107 38-39 0.6016220080697181 1107 40-41 0.43042319366031023 1107 42-43 0.519944735667849 1107 44-45 0.583921315749599 1107 46-47 0.5592578725382538 1107 48-49 0.6623046922276146 1107 50-51 0.5641187785213049 1107 52-53 0.6974036129200627 1107 54-55 0.7641907795256841 1107 56-57 0.8139120523483072 1107 58-59 0.7730978107415183 1107 60-61 0.8706225469799804 1107 62-63 0.9314387718453716 1107 64-65 0.8501381927415963 1107 66-67 1.0062721189199095 1107 68-69 0.8975353389299983 1107 70-71 0.8222065955910232 1107 72-73 0.6812237154129015 1107 74-75 0.824415709208477 1107 76-77 0.7303420111561998 1107 78-79 0.6838789368518157 1107 80-81 0.7699342511044129 1107 82-83 0.8007019414397192 1107 84-85 0.9006730373643315 1107 86-87 1.055449748660191 1107 88-89 0.9056057577263985 1107 90-91 1.0712801413760644 1107 92-93 0.9458576085274579 1107 94-95 0.7957584725696378 1107 96-97 0.7821124431293249 1107 98-99 1.24976977265095 1107 100-101 1.4599094461664883 1108 1 0.016838293912233127 1108 2 0.03224689628462585 1108 3 0.03612681737978107 1108 4 -0.033181835206463006 1108 5 -0.16700818682319607 1108 6 0.11875257068149381 1108 7 0.11265576643541664 1108 8 0.05044713756445418 1108 9 -0.014828251536982862 1108 10-11 -0.05973496713040305 1108 12-13 0.029874214734128657 1108 14-15 0.062494834237377006 1108 16-17 0.020760147134922136 1108 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-3.5134519854438544 2316 88-89 -3.363185874533439 2316 90-91 -3.8677427047566084 2316 92-93 -3.8247837347150373 2316 94-95 -3.9528817696544216 2316 96-97 -4.278672032510645 2316 98-99 -4.562250596190228 2316 100-101 -4.604535915793324 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1510.0 3 552.0 4 522.0 5 585.0 6 735.0 7 938.0 8 1170.0 9 1650.0 10 2100.0 11 3281.0 12 4849.0 13 6571.0 14 7588.0 15 10210.0 16 13700.0 17 19039.0 18 15665.0 19 7772.0 20 6971.0 21 6976.0 22 7584.0 23 8268.0 24 8995.0 25 10300.0 26 11975.0 27 14066.0 28 16904.0 29 20083.0 30 24445.0 31 30166.0 32 38244.0 33 48988.0 34 66565.0 35 95709.0 36 133965.0 37 185834.0 38 167237.0 39 30095.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.67230740146488 19.84090898074136 15.394116249872756 19.092667367921006 2 14.894184228307306 22.678436576941458 40.27513748634346 22.15224170840778 3 19.23538860168921 29.338635083641186 31.40404940243833 20.02192691223128 4 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33.466328386453796 19.913096756930738 24-25 20.45015631504545 26.35920911517107 33.810331283217856 19.380303286565628 26-27 20.20000251989994 25.771065152012962 34.75363616715465 19.275296160932438 28-29 19.20699158569214 26.905286092767223 34.33219821188083 19.555524109659807 30-31 19.507039386837715 26.5384302254581 34.00305883310751 19.95147155459668 32-33 19.562265445691953 26.701003349668134 33.841970488803426 19.894760715836487 34-35 20.40152807076462 26.207537907368827 33.59831782340538 19.79261619846117 36-37 20.439229627791708 25.846247338288613 34.01945799463248 19.6950650392872 38-39 20.061427317710944 26.630312316913784 33.6581576553722 19.65010271000307 40-41 19.622719778861804 26.968809395271933 33.65713499258059 19.75133583328568 42-43 19.34585797927657 27.38212349737686 32.39569714056983 20.876321382776737 44-45 19.871931241789145 26.97454277253258 32.167898587841805 20.985627397836478 46-47 20.774996656322266 27.438510251037506 31.42766316662758 20.35882992601265 48-49 20.535049279834546 27.630192142653964 30.792688393180345 21.04207018433115 50-51 20.127534653100703 28.18565261805121 31.06732131676937 20.619491412078723 52-53 20.38196867488563 28.961240986275875 29.74887570752888 20.907914631309605 54-55 19.788698264999518 28.183241252302025 30.37956770378986 21.648492778908597 56-57 20.782432463867007 28.452261413684017 29.333584687674463 21.431721434774516 58-59 21.352768628142734 27.73861890129104 29.732482817074228 21.176129653491998 60-61 20.77636546019316 28.099961717201577 30.743170881812766 20.380501940792502 62-63 20.75667193452541 28.192494440155524 30.54917063936879 20.50166298595028 64-65 20.44485688740711 28.853330490384455 30.328481744149137 20.3733308780593 66-67 20.5756707450655 28.39017306860899 29.54651642959479 21.487639756730715 68-69 20.325853574349406 28.584081478906732 30.03014882437037 21.059916122373494 70-71 21.09002154210019 28.5904348808445 29.428312516091122 20.89123106096419 72-73 20.724726734911055 28.132493104163476 30.001298723955255 21.141481436970214 74-75 20.12287677234502 29.77607484462297 29.29133339278306 20.80971499024895 76-77 20.425380118716365 28.595212280508736 29.55171897363221 21.42768862714269 78-79 20.314204743461175 28.69249705672368 29.74214039803662 21.25115780177852 80-81 20.52679435234555 29.343264980517386 29.044976104398685 21.08496456273838 82-83 20.264632562756685 28.929948179596888 29.52614213554736 21.279277122099067 84-85 20.15102082751776 28.97436493905276 28.99995347538848 21.874660758041003 86-87 20.249038923419732 29.18856586676723 28.964868052219746 21.597527157593294 88-89 20.39590769975125 28.53580956637965 28.687455246829042 22.380827487040065 90-91 20.00915258148367 28.10099447063854 29.431948520607364 22.457904427270428 92-93 20.43107134235079 28.913367855754323 29.34728967323278 21.30827112866211 94-95 19.944557660759763 28.546698297232005 29.252473460130226 22.256270581878013 96-97 19.363564018234566 28.57894068531803 29.628164902046883 22.42933039440052 98-99 19.782272747543807 28.91832464996694 28.665337177670015 22.634065424819237 100-101 19.572110877964118 28.368045941003516 29.394706329350868 22.665136851681496 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1602.0 1 1137.5 2 841.5 3 976.5 4 823.0 5 856.5 6 1459.0 7 3039.5 8 5062.0 9 5699.0 10 4586.0 11 3569.0 12 3565.5 13 3622.5 14 3548.0 15 3621.5 16 3674.5 17 3664.0 18 3636.0 19 3817.0 20 4178.0 21 4554.0 22 5071.0 23 5728.0 24 6328.5 25 7046.0 26 8327.5 27 9906.5 28 11037.0 29 12291.0 30 14413.0 31 16514.5 32 19347.0 33 22598.5 34 25654.0 35 27716.5 36 30760.0 37 34666.5 38 34561.0 39 33070.5 40 32168.0 41 31403.5 42 30134.5 43 30121.5 44 31826.0 45 32486.5 46 32136.5 47 37267.5 48 56305.0 49 55209.0 50 34568.0 51 25994.5 52 22485.5 53 21668.0 54 21139.5 55 19640.5 56 18075.5 57 16487.5 58 15762.0 59 15112.5 60 13814.5 61 12242.0 62 10135.5 63 7231.0 64 4675.5 65 3356.5 66 2426.5 67 1744.0 68 1254.5 69 834.5 70 593.5 71 469.0 72 358.5 73 305.5 74 208.0 75 154.5 76 128.5 77 61.0 78 20.5 79 7.0 80 6.0 81 9.0 82 7.0 83 3.5 84 2.0 85 1.0 86 1.0 87 1.5 88 1.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03198247747900544 2 0.02500448239267698 3 0.019964819274773095 4 0.04535696806113499 5 0.09236151829543086 6 0.10680209146019394 7 0.050590464375881335 8 0.07918086091014377 9 0.06483720434380194 10-11 0.06638786991854159 12-13 0.06546716223353993 14-15 0.05926449993458129 16-17 0.046665342139821576 18-19 0.058053042454315945 20-21 0.054467128312730484 22-23 0.03130406129005684 24-25 0.008189452566593817 26-27 0.0027136647557944008 28-29 0.005863454204484331 30-31 0.004748913322640202 32-33 0.006881078487907231 34-35 0.0054757878107994165 36-37 0.005233496314746345 38-39 0.002568289858162558 40-41 0.006008829102116173 42-43 0.0034889975431642304 44-45 0.00247137325974133 46-47 0.0016475821731608865 48-49 3.392080944743001E-4 50-51 0.0016475821731608865 52-53 0.005330412913167573 54-55 0.011145408818441291 56-57 0.006881078487907231 58-59 0.004797371621850816 60-61 0.0019383319684245725 62-63 0.008383285763436275 64-65 0.002180623464477644 66-67 0.01948023628266695 68-69 0.041819512218760145 70-71 0.05606625218668075 72-73 0.0031982477479005444 74-75 0.012696074393180948 76-77 0.01904411158977142 78-79 0.021951609542408282 80-81 0.07438348928829296 82-83 0.026312856471363565 84-85 0.009982409637386547 86-87 0.028057355242945684 88-89 0.04492084336823946 90-91 0.03992963854954619 92-93 0.03954197215586128 94-95 0.010903117322388219 96-97 0.014343656566341836 98-99 0.033048560061638955 100-101 0.07302665691039575 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1031815.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.04671588849099 #Duplication Level Percentage of deduplicated Percentage of total 1 73.24700030212878 30.797958113878227 2 13.727889933014922 11.544253755239081 3 4.967208474487886 6.265644104570907 4 2.45163123620855 4.1233216820884335 5 1.3869005492589843 2.915730668014231 6 0.8204220762617771 2.0697632369534897 7 0.5730561657672227 1.6866590853113688 8 0.4053285417033905 1.3634187227599066 9 0.3016182210984657 1.1413850084387323 >10 1.8174197836774084 14.737030077472859 >50 0.18452438887821296 5.356734989378952 >100 0.10497657846769186 7.854444900550869 >500 0.005318196693720702 1.6352894370279016 >1k 0.00554942263692595 4.345767095464338 >5k 9.249037728209917E-4 2.8414369254802287 >10k+ 2.3122594320524792E-4 1.3211621973705205 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13589 1.3169996559460755 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9666 0.9367958403395958 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7514 0.7282313205371118 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6923 0.6709536108701657 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5123 0.49650373371195416 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3769 0.36527865944961063 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3163 0.3065472008063461 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2792 0.2705911427920703 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2448 0.23725183293516763 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2371 0.22978925485673302 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2229 0.2160270978809186 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2084 0.20197419110984044 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2065 0.2001327757398371 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2008 0.19460852962982705 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1992 0.1930578640550874 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1949 0.18889045032297458 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1809 0.17532212654400256 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1806 0.1750313767487389 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1710 0.16572738330030093 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1665 0.16136613637134564 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1635 0.15845863841870877 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1302 0.12618541114443965 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1251 0.12124266462495699 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1221 0.11833516667232014 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1179 0.11426466953862854 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1085 0.10515450928703304 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1080 0.1046699262949269 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1071 0.10379767690913584 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.691659842122862E-5 0.0 18-19 0.0 0.0 0.0 9.691659842122862E-5 0.0 20-21 0.0 0.0 0.0 2.4229149605307156E-4 0.0 22-23 0.0 0.0 0.0 6.29957889737986E-4 0.0 24-25 0.0 0.0 0.0 0.0015022072755290434 0.0 26-27 0.0 0.0 0.0 0.00247137325974133 0.0 28-29 0.0 0.0 0.0 0.004312788629744673 0.0 30-31 0.0 0.0 0.0 0.008577118960278733 0.0 32-33 0.0 0.0 0.0 0.013568323778972005 0.0 34-35 0.0 0.0 0.0 0.019092569888982037 0.0 36-37 0.0 0.0 0.0 0.023696108313990397 0.0 38-39 0.0 0.0 0.0 0.028541938235051828 0.0 40-41 0.0 0.0 0.0 0.03295164346321773 0.0 42-43 0.0 0.0 0.0 0.0396388887542825 0.0 44-45 0.0 0.0 0.0 0.04458163527376516 0.0 46-47 0.0 0.0 0.0 0.05257725464351652 0.0 48-49 0.0 0.0 0.0 0.06391649665880027 0.0 50-51 0.0 0.0 0.0 0.07239669902065776 0.0 52-53 0.0 0.0 0.0 0.09226460169700965 0.0 54-55 0.0 0.0 0.0 0.13277573983708318 0.0 56-57 0.0 0.0 0.0 0.20371868988142255 0.0 58-59 0.0 0.0 0.0 0.27839292896497914 0.0 60-61 0.0 0.0 0.0 0.34284246691509623 0.0 62-63 0.0 0.0 0.0 0.41867970517970765 0.0 64-65 0.0 0.0 0.0 0.5121557643569826 0.0 66-67 0.0 0.0 0.0 0.6646540319727858 0.0 68-69 0.0 0.0 0.0 0.8582933956184007 0.0 70-71 0.0 0.0 0.0 1.0105009134389402 0.0 72-73 0.0 0.0 0.0 1.1747745477629226 0.0 74-75 0.0 0.0 0.0 1.3512112151887692 0.0 76-77 0.0 0.0 0.0 1.5374849173543708 0.0 78-79 0.0 0.0 0.0 1.6884325193954344 0.0 80-81 0.0 0.0 0.0 1.8553713601760005 0.0 82-83 0.0 0.0 0.0 2.0196449944999832 0.0 84-85 0.0 0.0 0.0 2.335011605762661 0.0 86-87 0.0 0.0 0.0 2.76091159752475 0.0 88-89 0.0 0.0 0.0 3.22790422701744 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 8495 0.0 25.44719 12-13 GTACATG 10140 0.0 25.025305 1 TACATGG 10225 0.0 24.588476 2 AGTACTT 8780 0.0 23.809483 12-13 ACATGGG 10225 0.0 23.612375 3 GATATAG 415 0.0 22.901184 1 GTACTTT 9460 0.0 22.72856 14-15 AGGCTTA 445 0.0 22.426237 6 GTATAGG 365 0.0 22.132584 1 CATGGGA 7685 0.0 21.95451 4 CATATAG 305 9.094947E-12 21.815615 3 CATGGGG 2180 0.0 21.80132 4 CCGTATC 55 0.002878406 21.590557 92-93 CATGGGT 705 0.0 21.572483 4 CTTTGCG 310 1.0913936E-11 21.46375 2 TTTGCGT 315 1.4551915E-11 21.123056 3 ATGGGAT 2830 0.0 20.992435 5 ACTTTTT 10410 0.0 20.905943 16-17 TACTTTT 10080 0.0 20.529203 14-15 GTATCAA 24205 0.0 19.534128 1 >>END_MODULE