##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936980_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1031815 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02867665230686 31.0 31.0 34.0 30.0 34.0 2 32.12283112767308 33.0 31.0 34.0 30.0 34.0 3 32.21879988176175 33.0 31.0 34.0 30.0 34.0 4 35.942971365991 37.0 35.0 37.0 35.0 37.0 5 35.79677461560454 37.0 35.0 37.0 35.0 37.0 6 35.91963869492108 37.0 35.0 37.0 35.0 37.0 7 35.87152541880085 37.0 35.0 37.0 35.0 37.0 8 35.82985709647563 37.0 35.0 37.0 35.0 37.0 9 37.4701104364639 39.0 37.0 39.0 35.0 39.0 10-11 37.389135649316984 39.0 37.0 39.0 34.0 39.0 12-13 37.284040743737975 39.0 37.0 39.0 34.0 39.0 14-15 38.29859567848888 40.0 38.0 41.0 33.5 41.0 16-17 38.33633015608418 40.0 37.5 41.0 33.5 41.0 18-19 38.582280738310644 40.0 38.0 41.0 34.0 41.0 20-21 38.627811187082955 40.0 38.0 41.0 34.0 41.0 22-23 38.527930394499016 40.0 38.0 41.0 34.0 41.0 24-25 38.398148892970156 40.0 38.0 41.0 34.0 41.0 26-27 38.143251454960435 40.0 37.0 41.0 33.5 41.0 28-29 37.98070002859039 40.0 37.0 41.0 33.0 41.0 30-31 37.82655999379733 40.0 37.0 41.0 33.0 41.0 32-33 37.543578063897115 40.0 36.0 41.0 33.0 41.0 34-35 37.33940338142012 39.0 35.0 41.0 33.0 41.0 36-37 37.097234484864046 39.0 35.0 41.0 32.5 41.0 38-39 36.857546653227566 39.0 35.0 41.0 31.5 41.0 40-41 36.492889713756824 39.0 35.0 41.0 30.5 41.0 42-43 36.16264398172153 39.0 35.0 41.0 30.0 41.0 44-45 35.72765999718942 39.0 35.0 40.0 29.5 41.0 46-47 35.06584271405242 38.5 35.0 40.0 25.5 41.0 48-49 34.45449668787525 38.0 34.0 40.0 22.5 41.0 50-51 34.09974704767812 38.0 34.0 40.0 20.0 41.0 52-53 33.828728502687014 38.0 34.0 40.0 18.5 41.0 54-55 33.450821610463116 38.0 33.0 40.0 14.5 41.0 56-57 33.25025997877526 38.0 33.0 40.0 13.5 41.0 58-59 32.94490242921454 37.0 33.0 40.0 10.5 41.0 60-61 32.52439100032467 36.5 32.0 39.5 8.0 41.0 62-63 32.17092114380969 36.0 31.5 39.0 7.0 40.0 64-65 31.845931198906783 36.0 31.0 39.0 3.5 40.0 66-67 31.511893120375262 35.0 31.0 39.0 2.0 40.0 68-69 31.061856534359357 35.0 31.0 38.0 2.0 40.0 70-71 30.601345202386085 35.0 30.0 37.5 2.0 39.5 72-73 30.180457252511353 34.5 29.5 37.0 2.0 39.0 74-75 29.7222467205846 34.0 29.0 36.0 2.0 39.0 76-77 28.287959566395138 32.5 27.0 35.0 2.0 37.0 78-79 28.943462248562 34.0 29.0 35.0 2.0 37.0 80-81 28.79431923358354 34.0 29.0 35.0 2.0 37.0 82-83 28.441348982133427 34.0 28.5 35.0 2.0 36.5 84-85 28.000221939010384 34.0 27.5 35.0 2.0 36.0 86-87 27.65579440112811 33.0 27.0 35.0 2.0 36.0 88-89 27.26216521372533 33.0 26.0 35.0 2.0 35.0 90-91 26.758044319960458 33.0 25.5 35.0 2.0 35.0 92-93 26.307739759549918 32.5 25.0 34.5 2.0 35.0 94-95 25.76389323667518 32.0 23.5 34.0 2.0 35.0 96-97 24.99649307288613 32.0 19.0 34.0 2.0 35.0 98-99 24.20196353028401 31.5 7.5 34.0 2.0 35.0 100-101 21.26992144909698 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13524120473596213 1101 2 0.15567825811341152 1101 3 0.07008286840721212 1101 4 0.02300128389777001 1101 5 -0.003434498441677647 1101 6 0.03635475073202343 1101 7 0.016171376859482223 1101 8 0.06209597892111418 1101 9 0.09978875868906556 1101 10-11 0.1685230333469363 1101 12-13 0.20771546906295413 1101 14-15 0.31293661938973827 1101 16-17 0.3439380870486275 1101 18-19 -0.03239211340105186 1101 20-21 -0.08505078525911358 1101 22-23 0.010310740200281998 1101 24-25 0.1359991696570617 1101 26-27 0.22539651544335015 1101 28-29 0.19735252754227162 1101 30-31 0.12333372082721894 1101 32-33 0.3259375795741306 1101 34-35 0.15351473699009688 1101 36-37 0.34088120250869736 1101 38-39 0.39944714868143194 1101 40-41 0.3955730599943479 1101 42-43 0.3929759579072183 1101 44-45 0.1420868062153957 1101 46-47 0.21245743975031672 1101 48-49 0.38410814783114944 1101 50-51 0.2995223527035762 1101 52-53 0.449427601438245 1101 54-55 0.24631035427380965 1101 56-57 0.3526864453949443 1101 58-59 0.2937244819246345 1101 60-61 0.1339139930050237 1101 62-63 0.027044724042269763 1101 64-65 0.28049338318365 1101 66-67 0.34671749185505263 1101 68-69 0.027063202265985353 1101 70-71 -0.14068425957971087 1101 72-73 -0.013334247701212831 1101 74-75 0.07779647755280195 1101 76-77 0.13716911634933382 1101 78-79 0.028673111000639295 1101 80-81 0.07128244318122867 1101 82-83 0.25197043088715887 1101 84-85 0.16031393349731005 1101 86-87 -0.07960094314464428 1101 88-89 0.15285257658097606 1101 90-91 0.22735827050762936 1101 92-93 0.18861548573849163 1101 94-95 -0.011961378670481082 1101 96-97 -0.27160609258178425 1101 98-99 -0.3054679161036411 1101 100-101 0.07863959780606677 1102 1 0.009946577074678942 1102 2 0.13607433677865544 1102 3 0.08690497247764029 1102 4 0.08524444518720742 1102 5 -0.07094494631242299 1102 6 0.1628393576122491 1102 7 0.24606031519066818 1102 8 0.16927668235756244 1102 9 0.34056274979862877 1102 10-11 0.23814507502832782 1102 12-13 0.2762392801159095 1102 14-15 0.2824393879034588 1102 16-17 0.2713888065869554 1102 18-19 0.2874301695791317 1102 20-21 0.18203257292279318 1102 22-23 -0.031822047534760145 1102 24-25 -0.037976801387081593 1102 26-27 0.12539330424268513 1102 28-29 0.0950767037546214 1102 30-31 0.06088963012456361 1102 32-33 0.13295634674832257 1102 34-35 0.31839245905609204 1102 36-37 0.2294809813945946 1102 38-39 0.2747814576081069 1102 40-41 0.3958120650726258 1102 42-43 0.4668555929313243 1102 44-45 0.5588795507368616 1102 46-47 0.5489752137460187 1102 48-49 0.6852348205226377 1102 50-51 0.4415606393904028 1102 52-53 0.5505116110351409 1102 54-55 0.828990514099857 1102 56-57 0.6183674263708738 1102 58-59 0.45323215898794444 1102 60-61 0.7055554211635879 1102 62-63 0.7574235998467884 1102 64-65 0.9301885484916745 1102 66-67 0.8391184148458706 1102 68-69 0.9470781572862421 1102 70-71 0.5925961653997582 1102 72-73 0.5568534240406819 1102 74-75 0.6788869095510393 1102 76-77 0.6949051281298502 1102 78-79 0.28544530383782885 1102 80-81 0.6169163571302256 1102 82-83 0.9387058784138453 1102 84-85 0.7094104392522418 1102 86-87 0.7411324033393178 1102 88-89 0.6161173584257646 1102 90-91 0.5665784992368614 1102 92-93 0.33039183019939955 1102 94-95 0.5785586982148381 1102 96-97 0.9050833430267673 1102 98-99 0.7454942972393575 1102 100-101 0.8763270745822069 1103 1 0.11713027083555261 1103 2 0.2893084193751534 1103 3 0.2371704104947554 1103 4 0.1548593381242469 1103 5 0.08474554763310493 1103 6 0.028335966397449397 1103 7 0.1114974445539687 1103 8 0.08958135903807829 1103 9 0.15374498764529676 1103 10-11 0.2286838520603922 1103 12-13 0.12393689383701201 1103 14-15 0.16677987652246173 1103 16-17 0.16727673670306586 1103 18-19 0.017787312436269076 1103 20-21 0.24486681176374958 1103 22-23 0.2584854699539605 1103 24-25 0.3001162173267389 1103 26-27 0.33957115659167414 1103 28-29 0.16150944762024722 1103 30-31 0.11153813337374174 1103 32-33 0.17166901675293644 1103 34-35 0.02163453142568983 1103 36-37 0.042577994994964286 1103 38-39 0.02603096934419824 1103 40-41 0.23343191166899402 1103 42-43 -0.04173518864602954 1103 44-45 0.030040908643179876 1103 46-47 -0.1315616990056654 1103 48-49 0.06460389477426531 1103 50-51 0.07840769737839537 1103 52-53 0.13874622628317468 1103 54-55 0.2457366346211458 1103 56-57 0.261165977558683 1103 58-59 0.10213756007455288 1103 60-61 -0.215053754646938 1103 62-63 0.10668321420707372 1103 64-65 0.15150171208671637 1103 66-67 0.313339855845836 1103 68-69 0.2133382332778595 1103 70-71 0.23783337288528728 1103 72-73 0.3712949389016593 1103 74-75 0.2558954497307191 1103 76-77 0.42876489873458823 1103 78-79 0.32938195381474955 1103 80-81 0.36532594840894816 1103 82-83 0.3490641753493229 1103 84-85 0.3208380341529917 1103 86-87 0.10656997657890699 1103 88-89 0.4426762519309655 1103 90-91 0.12632380302579094 1103 92-93 0.3142888864118909 1103 94-95 0.3230222293394398 1103 96-97 0.3417976820062023 1103 98-99 0.12792301070834 1103 100-101 0.6262871257167539 1104 1 -0.0382223420513057 1104 2 0.010630083505034804 1104 3 -0.06383161893193545 1104 4 -0.055528789729635264 1104 5 -0.1857770297387873 1104 6 0.037189340319315534 1104 7 -0.02433462038768397 1104 8 0.00936826923594225 1104 9 0.06309303432210811 1104 10-11 0.03574245175597213 1104 12-13 0.12209138394355534 1104 14-15 0.11222000843520163 1104 16-17 0.005328487083588129 1104 18-19 0.1617941617513381 1104 20-21 0.10928842515796333 1104 22-23 0.07251388192757702 1104 24-25 0.10122606004616586 1104 26-27 0.22592960408025675 1104 28-29 0.21775627664104746 1104 30-31 0.16052976198865565 1104 32-33 0.032317164901080275 1104 34-35 0.17754523665146138 1104 36-37 9.366961618866299E-4 1104 38-39 0.0918287877105115 1104 40-41 0.2990141034498137 1104 42-43 0.07524857594047063 1104 44-45 0.3403783008298902 1104 46-47 -0.2763105574531508 1104 48-49 -0.5043649027904209 1104 50-51 -0.1987866202268762 1104 52-53 -0.07547244444234025 1104 54-55 -0.12618751555643115 1104 56-57 -0.2551962751049359 1104 58-59 -0.328798512984811 1104 60-61 -0.38348348574789526 1104 62-63 -0.2589180034489402 1104 64-65 -0.07183669481333155 1104 66-67 -0.4755236652094652 1104 68-69 -0.246808900156946 1104 70-71 -0.1893482608031931 1104 72-73 -0.21392195607550946 1104 74-75 -0.4604985127248895 1104 76-77 -0.1929372828990985 1104 78-79 -0.05884737140087637 1104 80-81 -0.11036532505629992 1104 82-83 -0.23079376478258595 1104 84-85 -0.08917591815547965 1104 86-87 -0.2349635089614317 1104 88-89 0.023054232651414708 1104 90-91 -0.36320562366116604 1104 92-93 -0.4029992921776575 1104 94-95 -0.20128649719530856 1104 96-97 0.0268623699818491 1104 98-99 -0.26559550781017904 1104 100-101 0.5314241120181222 1105 1 0.0037119344663949505 1105 2 0.18058042908978678 1105 3 0.03506051371272889 1105 4 0.06137117783280388 1105 5 0.008364248300985366 1105 6 0.11925885650067158 1105 7 0.052587304906126064 1105 8 0.11817880894700039 1105 9 0.24562416190151737 1105 10-11 0.07738603785274023 1105 12-13 0.04996148400094569 1105 14-15 0.1630534041484495 1105 16-17 0.09095615382510402 1105 18-19 -0.04147953637976087 1105 20-21 -1.6961810874960292E-4 1105 22-23 -0.03292315178252636 1105 24-25 -0.08407473592158965 1105 26-27 -4.3339714238754823E-4 1105 28-29 -0.003601359908593338 1105 30-31 0.2084188255413153 1105 32-33 0.14825523411784758 1105 34-35 0.1670504391366805 1105 36-37 0.23664296160091425 1105 38-39 0.2618764459683618 1105 40-41 0.405267066495945 1105 42-43 0.4048191525804441 1105 44-45 0.21454303371907457 1105 46-47 0.18701753596701565 1105 48-49 0.14891628093091214 1105 50-51 0.1655358090966743 1105 52-53 0.29832424692677506 1105 54-55 0.48288751500972893 1105 56-57 0.5291076897626894 1105 58-59 0.21156378958274757 1105 60-61 0.18684363454553932 1105 62-63 0.1863735602908605 1105 64-65 0.2390548389841065 1105 66-67 0.27047403920952817 1105 68-69 0.15866306630761073 1105 70-71 0.08882001526537309 1105 72-73 0.09645431959382478 1105 74-75 0.03900868591772522 1105 76-77 -0.07006630952284709 1105 78-79 0.13745724221790567 1105 80-81 0.21263165575563647 1105 82-83 0.2360101377051258 1105 84-85 0.0364497524286449 1105 86-87 0.0072302680178850665 1105 88-89 0.3844947097330298 1105 90-91 0.2666319390306491 1105 92-93 0.3929622318885144 1105 94-95 0.350124536564703 1105 96-97 0.6363104324615776 1105 98-99 0.7871367326269798 1105 100-101 1.1356222805437106 1106 1 -0.010187651113241714 1106 2 0.08046551606916097 1106 3 0.13963747487456857 1106 4 0.1734515401057095 1106 5 0.0067156481267005574 1106 6 0.06983839883947951 1106 7 0.13676021931338767 1106 8 0.15010122694049244 1106 9 0.24660301825500142 1106 10-11 0.2151345285278481 1106 12-13 0.30260553131437007 1106 14-15 0.41235309835407463 1106 16-17 0.3552814240366118 1106 18-19 0.22846564417164927 1106 20-21 0.23812078001771653 1106 22-23 0.2792997166071345 1106 24-25 0.3210836012974809 1106 26-27 0.2648947424018431 1106 28-29 0.28874880346698717 1106 30-31 0.37632587173530396 1106 32-33 0.34580390187775123 1106 34-35 0.27333013882599033 1106 36-37 0.2659143466870191 1106 38-39 0.36874118915174137 1106 40-41 0.5242876163669763 1106 42-43 0.33933881440783864 1106 44-45 0.020049253517299803 1106 46-47 0.37543734222156644 1106 48-49 0.42253108130883277 1106 50-51 0.5671598723721871 1106 52-53 0.38224698975038507 1106 54-55 0.7144171679828872 1106 56-57 0.5852897657764302 1106 58-59 0.3827623603302257 1106 60-61 0.3632735419689581 1106 62-63 0.35473903608840374 1106 64-65 0.3908788648866839 1106 66-67 0.4014386449513374 1106 68-69 0.4825527498078088 1106 70-71 0.3744753600306332 1106 72-73 0.37612475673483203 1106 74-75 0.314073870241355 1106 76-77 0.3890566142276448 1106 78-79 0.3300282376825088 1106 80-81 0.3053152066029021 1106 82-83 0.41713349106964515 1106 84-85 0.5262848400192617 1106 86-87 0.3600202269251298 1106 88-89 0.5135286719444601 1106 90-91 0.29524580508537923 1106 92-93 0.526940248224328 1106 94-95 0.7645606157603773 1106 96-97 0.7003853564495834 1106 98-99 0.7632315935001408 1106 100-101 1.3197594811018618 1107 1 0.2325800062852892 1107 2 0.2810279960947284 1107 3 0.19354607187041495 1107 4 0.2784307666956707 1107 5 0.15659739948495854 1107 6 0.18129892936041614 1107 7 0.23480432286084607 1107 8 0.09307342253013928 1107 9 0.2552000141003248 1107 10-11 0.1887235346000793 1107 12-13 0.275881338281458 1107 14-15 0.3924809347235225 1107 16-17 0.4064830859094144 1107 18-19 0.338382550531513 1107 20-21 0.2047874466843851 1107 22-23 0.13065478212327264 1107 24-25 0.3711347358452599 1107 26-27 0.3965949661154866 1107 28-29 0.4913374899482861 1107 30-31 0.40126035559596573 1107 32-33 0.29326954585346954 1107 34-35 0.3940683938595484 1107 36-37 0.49038222779919494 1107 38-39 0.3826050434182733 1107 40-41 0.4275985783356546 1107 42-43 0.5828415309306862 1107 44-45 0.43990863351090326 1107 46-47 0.4060573486133876 1107 48-49 0.39750072017108806 1107 50-51 0.3379844169551163 1107 52-53 0.5379261204630694 1107 54-55 0.5424165288010343 1107 56-57 0.3700548664475747 1107 58-59 0.4647566076936016 1107 60-61 0.44195682736364716 1107 62-63 0.660651468175331 1107 64-65 0.7439884845734888 1107 66-67 0.6610647235707034 1107 68-69 0.5668832068228333 1107 70-71 0.4888122088641218 1107 72-73 0.6633187484254677 1107 74-75 0.49723142856669966 1107 76-77 0.17736278233247305 1107 78-79 0.20816502259781444 1107 80-81 0.18033917592217463 1107 82-83 0.43086311714826664 1107 84-85 0.2736290394439962 1107 86-87 0.4703398178487461 1107 88-89 0.4821736064283222 1107 90-91 0.5035327977347848 1107 92-93 0.5160613731843782 1107 94-95 0.6865804303976439 1107 96-97 0.7815199042284249 1107 98-99 1.0286902064755346 1107 100-101 1.6884299828596099 1108 1 -0.005543622570044704 1108 2 0.020947985770817468 1108 3 0.12165550534570002 1108 4 0.05777261725703653 1108 5 -0.07225535570925956 1108 6 0.016818351221296268 1108 7 -0.0046046331334750334 1108 8 0.034567808902572494 1108 9 0.1350683842125946 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-1.2740581602987504 2316 96-97 -1.3867267239025978 2316 98-99 -1.980528070430605 2316 100-101 -2.647075109350684 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 16.0 5 43.0 6 92.0 7 206.0 8 471.0 9 867.0 10 1611.0 11 2738.0 12 4444.0 13 6204.0 14 7617.0 15 11011.0 16 14955.0 17 20629.0 18 17447.0 19 8908.0 20 6824.0 21 6699.0 22 6896.0 23 7747.0 24 8558.0 25 9912.0 26 12289.0 27 14796.0 28 18207.0 29 22154.0 30 27760.0 31 34287.0 32 42890.0 33 54931.0 34 75136.0 35 104788.0 36 150094.0 37 203294.0 38 121609.0 39 5683.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.135583658228754 20.504859375696725 15.925045608144131 18.43451135793039 2 15.215259066855339 23.573339277753007 39.41421620888173 21.797185446509914 3 19.798101581111176 29.17786870787726 31.596249682595374 19.427780028416194 4 11.801824939548272 18.427043607623457 39.345716044058285 30.425415408769986 5 11.30826674161768 40.84533114941582 33.26497283833357 14.581429270632931 6 26.883695236064604 39.241433784157046 17.72023085533744 16.15464012444091 7 24.922418187774152 37.167842266144866 21.336597487531666 16.573142058549312 8 26.61252259368201 32.103429393835135 20.180555622858748 21.10349238962411 9 27.729874056880348 16.92832532963758 21.957521454911976 33.38427915857009 10-11 24.023310391548094 28.096910830386662 26.644857190900105 21.234921587165132 12-13 27.56228216748921 26.672633839783828 27.351573434028975 18.413510558697986 14-15 23.697187739879507 26.39081654015244 29.492240503679124 20.419755216288934 16-17 19.116895955185765 30.003052872850265 29.646884373652256 21.23316679831171 18-19 19.027340304901095 27.652038650526745 34.47708395925606 18.843537085316093 20-21 21.279200155844006 25.51305920424578 34.162110717085945 19.045629922824265 22-23 20.42029381606161 25.89571448785717 34.26844491481893 19.415546781262297 24-25 20.548251225034114 26.12128419240789 34.59810352313609 18.73236105942191 26-27 20.275990333505682 25.61267246972921 35.44087267877902 18.670464517986083 28-29 19.240328566811186 26.742549169448576 35.07030575747869 18.946816506261545 30-31 19.559862746646807 26.35425262971203 34.67876251012173 19.40712211351944 32-33 19.642454765323382 26.536627016413334 34.4694559627297 19.351462255533583 34-35 20.430358155289465 25.887392604294373 34.48176271909208 19.200486521324077 36-37 20.472804578016778 25.618712144450406 34.750225455405385 19.158257822127435 38-39 20.110988662698563 26.34999374834622 34.52090523946742 19.0181123494878 40-41 19.808607524763605 26.723663787735397 34.18480112307879 19.282927564422206 42-43 19.513430217626222 27.262978343986084 33.014493877293894 20.2090975610938 44-45 20.11775480832134 26.698697912880824 32.707730627395684 20.475816651402155 46-47 20.95353426101991 27.51101458208553 31.74889078845606 19.786560368438497 48-49 20.922995363992268 27.56498382218897 31.108498669092167 20.403522144726598 50-51 20.443644328573022 28.12138070062367 31.343096254585458 20.091878716217852 52-53 20.734166885537924 28.945424976909486 30.074170704074106 20.246237433478484 54-55 20.04433934377771 28.071359691417552 30.59778157906214 21.2865193857426 56-57 21.00457351200947 28.41576606568451 29.604065085563153 20.975595336742874 58-59 21.727995549585486 27.615200026361343 29.94401122682964 20.71279319722353 60-61 20.973063867008143 28.076284718099814 30.95850183055217 19.992149584339874 62-63 21.03080494080819 28.01379123195534 30.86207314295683 20.093330684279643 64-65 20.702461537417822 28.78293530474712 30.49491938715728 20.01968377067777 66-67 20.902729655994534 28.292523369014795 29.767690913584318 21.037056061406357 68-69 20.78635296671687 28.46254266757381 30.144241113223895 20.60686325248543 70-71 21.433614570103163 28.561369978915724 29.625711673110832 20.37930377787028 72-73 21.097486219637773 27.99248894542371 30.21697256042159 20.69305227451693 74-75 20.580578630542725 29.674950401296403 29.344647749861647 20.399823218299233 76-77 20.78172928286563 28.624656551804346 29.648289664329365 20.945324501000666 78-79 20.72009207099158 28.599139863104973 29.815882246047607 20.864885819855836 80-81 20.85336122013141 29.34113078187869 29.13542041722617 20.67008758076373 82-83 20.696065038822386 28.794710677161444 29.609343539948313 20.899880744067854 84-85 20.35248434638621 28.851157284351814 29.15863249370875 21.637725875553226 86-87 20.45357077957478 29.067635834999088 28.998340299230723 21.480453086195407 88-89 20.57803737117586 28.5545576619924 28.773401810164817 22.09400315666692 90-91 20.393304109238542 28.05551867276218 29.36643545193483 22.184741766064448 92-93 20.73506394072581 29.004521159316347 29.23716945382651 21.02324544613133 94-95 20.292348919137638 28.65247161555124 29.13787839874396 21.917301066567166 96-97 19.74093173859992 28.656161533146022 29.432561908020155 22.170344820233897 98-99 20.189589352292913 29.09143068145364 28.334313811955507 22.384666154297935 100-101 19.815951366190394 28.834819016420454 28.887833551804263 22.461396065584893 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2578.0 1 1812.5 2 1064.0 3 1093.0 4 964.5 5 937.0 6 1564.5 7 3239.0 8 5065.0 9 5668.0 10 4754.0 11 3755.0 12 3526.0 13 3517.5 14 3673.0 15 3764.5 16 3791.0 17 3870.5 18 3952.0 19 4108.0 20 4408.0 21 4851.5 22 5376.5 23 5974.0 24 6526.0 25 7402.5 26 8784.0 27 10143.5 28 11182.5 29 12716.5 30 14735.5 31 16339.5 32 19077.0 33 22449.0 34 25430.5 35 27295.5 36 30368.5 37 33884.5 38 33947.5 39 33075.0 40 32273.0 41 31104.5 42 29543.5 43 29704.0 44 31596.5 45 32380.0 46 32004.5 47 37603.5 48 57579.0 49 55740.0 50 33833.5 51 25403.5 52 22149.5 53 21439.5 54 20817.5 55 19210.5 56 17664.0 57 16442.5 58 15843.0 59 15077.5 60 13675.0 61 11851.5 62 9793.5 63 7137.0 64 4765.5 65 3474.5 66 2322.0 67 1541.5 68 1185.5 69 838.5 70 605.0 71 477.0 72 372.0 73 333.0 74 251.0 75 192.5 76 127.0 77 41.5 78 17.5 79 12.0 80 7.5 81 4.5 82 2.0 83 1.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01948023628266695 2 0.0033920809447430017 3 8.722493857910576E-4 4 0.0 5 0.002907497952636858 6 0.0 7 9.691659842122862E-5 8 0.0 9 0.0 10-11 4.845829921061431E-5 12-13 0.005718079306852488 14-15 0.004555080125797745 16-17 0.0 18-19 4.845829921061431E-4 20-21 0.0013568323778972004 22-23 0.001259915779475972 24-25 6.784161889486002E-4 26-27 3.392080944743001E-4 28-29 4.845829921061431E-5 30-31 4.845829921061431E-5 32-33 1.4537489763184291E-4 34-35 0.0 36-37 0.0014052906771078147 38-39 0.008528660661068119 40-41 8.237910865804433E-4 42-43 0.0 44-45 9.691659842122862E-4 46-47 9.691659842122862E-5 48-49 4.845829921061431E-5 50-51 0.0019383319684245725 52-53 5.814995905273717E-4 54-55 0.0 56-57 3.876663936849145E-4 58-59 9.691659842122862E-5 60-61 0.002180623464477644 62-63 0.0 64-65 4.845829921061431E-5 66-67 0.0 68-69 9.691659842122862E-5 70-71 3.392080944743001E-4 72-73 2.4229149605307156E-4 74-75 0.003295164346321773 76-77 0.0 78-79 2.4229149605307156E-4 80-81 0.002374456661320101 82-83 4.845829921061431E-5 84-85 0.002374456661320101 86-87 2.4229149605307156E-4 88-89 0.0029559562518474726 90-91 0.0017444987715821152 92-93 0.0 94-95 0.0 96-97 1.9383319684245724E-4 98-99 5.814995905273717E-4 100-101 0.002180623464477644 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1031815.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.34961284292077 #Duplication Level Percentage of deduplicated Percentage of total 1 71.95709263117665 29.034408289701652 2 14.103422090135439 11.381352421945229 3 5.367924482069088 6.4978102386457115 4 2.6170095781441063 4.223812931373205 5 1.4268577194238508 2.878657828034263 6 0.9243392929922579 2.2378039564642034 7 0.6135275879744879 1.7328920450255123 8 0.42451636559702255 1.3703256797858938 9 0.3278870923152935 1.1907105508001803 >10 1.9208943660451727 14.917858355740144 >50 0.1885617073048194 5.2597421452784126 >100 0.11439482005125864 8.211406302447948 >500 0.006301409572085797 1.7793270957333622 >1k 0.006301409572085797 5.247719187207677 >5k 4.8472381323736905E-4 1.6282383151393507 >10k+ 4.8472381323736905E-4 2.407934656677251 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13985 1.3553786289208822 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10638 1.03099877400503 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8865 0.8591656450041917 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7785 0.7544957187092648 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4089 0.39629197094440377 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3728 0.36130507891434027 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3365 0.32612435368743425 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2968 0.2876484641142065 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2722 0.26380698090258425 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2660 0.2577981518004681 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2567 0.24878490814729384 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2551 0.2472342425725542 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2515 0.24374524502938996 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2502 0.24248532924991398 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2361 0.22882008887252073 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2158 0.20914601939301136 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2006 0.1944146964329846 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1943 0.1883089507324472 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1882 0.18239703822875225 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1694 0.16417671772556128 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1668 0.1616568861666093 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1503 0.1456656474271066 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1237 0.11988583224705979 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1145 0.11096950519230676 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1096 0.10622059186966655 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1096 0.10622059186966655 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1089 0.10554217568071796 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1056 0.10234392793281742 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1053 0.10205317813755373 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.691659842122862E-5 0.0 18-19 0.0 0.0 0.0 9.691659842122862E-5 0.0 20-21 0.0 0.0 0.0 2.4229149605307156E-4 0.0 22-23 0.0 0.0 0.0 6.29957889737986E-4 0.0 24-25 0.0 0.0 0.0 0.0015022072755290434 0.0 26-27 0.0 0.0 0.0 0.0024229149605307153 0.0 28-29 0.0 0.0 0.0 0.0042158720313234446 0.0 30-31 0.0 0.0 0.0 0.008528660661068117 0.0 32-33 0.0 0.0 0.0 0.01371369867660385 0.0 34-35 0.0 0.0 0.0 0.019286403085824494 0.0 36-37 0.0 0.0 0.0 0.02398685810925408 0.0 38-39 0.0 0.0 0.0 0.02907497952636858 0.0 40-41 9.691659842122862E-5 0.0 0.0 0.033726976250587556 0.0 42-43 9.691659842122862E-5 0.0 0.0 0.040414221541652334 0.0 44-45 9.691659842122862E-5 0.0 0.0 0.0454054263603456 0.0 46-47 9.691659842122862E-5 0.0 0.0 0.05344950402930758 0.0 48-49 9.691659842122862E-5 0.0 0.0 0.06474028774538071 0.0 50-51 9.691659842122862E-5 0.0 0.0 0.07331740670565945 0.0 52-53 9.691659842122862E-5 0.0 0.0 0.09328222598043254 0.0 54-55 9.691659842122862E-5 0.0 0.0 0.13437486371103347 0.0 56-57 9.691659842122862E-5 0.0 0.0 0.20565702184984713 0.0 58-59 9.691659842122862E-5 0.0 0.0 0.2805250941302462 0.0 60-61 9.691659842122862E-5 0.0 0.0 0.34531384017483757 0.0 62-63 9.691659842122862E-5 0.0 0.0 0.42163566143155506 0.0 64-65 9.691659842122862E-5 0.0 0.0 0.5155963036009362 0.0 66-67 9.691659842122862E-5 0.0 0.0 0.6683853210120032 0.0 68-69 9.691659842122862E-5 0.0 0.0 0.8633330587363044 0.0 70-71 9.691659842122862E-5 0.0 0.0 1.0164612842418457 0.0 72-73 9.691659842122862E-5 0.0 0.0 1.1821402092429358 0.0 74-75 9.691659842122862E-5 0.0 0.0 1.3598367924482586 0.0 76-77 9.691659842122862E-5 0.0 0.0 1.5477096184878103 0.0 78-79 9.691659842122862E-5 0.0 0.0 1.6988995120249268 0.0 80-81 9.691659842122862E-5 0.0 0.0 1.8668075187897055 0.0 82-83 9.691659842122862E-5 0.0 0.0 2.0323410688931642 0.0 84-85 9.691659842122862E-5 0.0 0.0 2.3487737627384755 0.0 86-87 9.691659842122862E-5 0.0 0.0 2.772977714028193 0.0 88-89 9.691659842122862E-5 0.0 0.0 3.240115718418515 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTATG 190 3.947207E-10 27.501432 7 GTACCGG 95 0.0012247127 25.006147 1 GTACATG 9630 0.0 24.964602 1 GAGTACT 9815 0.0 24.272692 12-13 TACATGG 9915 0.0 24.096247 2 AGGCTTA 420 0.0 23.751234 6 ATGGGTA 450 0.0 23.22118 5 CATGGGT 700 0.0 23.070395 4 AGTACTT 10065 0.0 22.96182 12-13 CGTCACT 145 2.3296108E-5 22.936674 1 ACATGGG 9950 0.0 22.865807 3 ATGGGAG 2355 0.0 22.185843 5 GTACTTT 10795 0.0 22.155012 14-15 TGGGTAT 290 1.05501385E-10 21.29421 6 CATATAG 315 1.4551915E-11 21.110163 3 CATGGGA 7310 0.0 21.052406 4 CATGGGG 2305 0.0 20.400385 4 ACTTTTT 12000 0.0 20.265757 16-17 ATGGGGA 1010 0.0 20.221865 5 ATGGGAT 2690 0.0 20.129208 5 >>END_MODULE