##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936978_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25324 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.70601010898752 33.0 31.0 34.0 30.0 34.0 2 31.791107250039488 33.0 31.0 34.0 30.0 34.0 3 31.669799399778867 33.0 31.0 34.0 30.0 34.0 4 35.332253988311486 37.0 35.0 37.0 33.0 37.0 5 35.29868899068078 37.0 35.0 37.0 33.0 37.0 6 35.42564365818986 37.0 35.0 37.0 33.0 37.0 7 35.39902858948034 37.0 35.0 37.0 33.0 37.0 8 35.38003474964461 37.0 35.0 37.0 33.0 37.0 9 36.98846943610804 39.0 37.0 39.0 34.0 39.0 10-11 36.84990522824199 39.0 37.0 39.0 33.0 39.0 12-13 36.82159216553467 39.0 37.0 39.0 33.0 39.0 14-15 37.91620597062075 40.0 38.0 41.0 33.0 41.0 16-17 37.78848128257779 40.0 37.5 41.0 33.0 41.0 18-19 37.67224767019428 40.0 37.0 41.0 33.0 41.0 20-21 37.64263149581424 40.0 37.0 41.0 33.0 41.0 22-23 37.492438003474966 40.0 36.0 41.0 33.0 41.0 24-25 37.346963354920234 40.0 36.0 41.0 33.0 41.0 26-27 37.17781551097772 40.0 35.5 41.0 33.0 41.0 28-29 36.919621702732584 39.5 35.0 41.0 32.5 41.0 30-31 36.51844100458064 39.0 35.0 41.0 32.0 41.0 32-33 36.102491707471174 38.0 35.0 41.0 31.0 41.0 34-35 35.834564049913126 38.0 35.0 41.0 31.0 41.0 36-37 35.51528194598009 38.0 35.0 41.0 30.0 41.0 38-39 35.160499921023536 38.0 35.0 40.0 30.0 41.0 40-41 34.804335807929235 38.0 35.0 40.0 26.5 41.0 42-43 34.31829489812036 38.0 35.0 40.0 23.5 41.0 44-45 33.701508450481754 38.0 35.0 40.0 14.0 41.0 46-47 32.96515163481283 37.0 34.5 40.0 2.0 41.0 48-49 32.34891802242932 37.0 33.0 40.0 2.0 41.0 50-51 31.467915811088297 36.0 32.0 39.5 2.0 40.5 52-53 31.458892749960512 37.0 33.0 39.5 2.0 40.5 54-55 31.681665613647134 38.0 33.0 40.0 2.0 41.0 56-57 31.417430105828466 38.0 33.0 40.0 2.0 41.0 58-59 31.092817090507026 37.0 32.5 40.0 2.0 41.0 60-61 30.75183620281156 36.5 31.5 40.0 2.0 41.0 62-63 30.332530405939032 36.0 31.0 40.0 2.0 41.0 64-65 29.882088137734954 36.0 29.5 40.0 2.0 41.0 66-67 29.40274048333597 35.0 28.5 39.0 2.0 41.0 68-69 28.911269941557414 35.0 27.0 39.0 2.0 41.0 70-71 28.431349707787078 35.0 26.0 38.5 2.0 40.5 72-73 27.925801611119887 35.0 24.0 37.5 2.0 40.0 74-75 27.410499921023536 35.0 20.5 37.0 2.0 39.5 76-77 26.901694045174537 35.0 15.5 36.5 2.0 39.0 78-79 26.3782182909493 35.0 4.5 36.0 2.0 39.0 80-81 25.907755488864318 34.0 2.0 36.0 2.0 37.5 82-83 25.397666245458854 34.0 2.0 35.0 2.0 37.0 84-85 24.994846785657874 34.0 2.0 35.0 2.0 36.5 86-87 24.61317327436424 34.0 2.0 35.0 2.0 36.0 88-89 24.185614436897804 33.5 2.0 35.0 2.0 36.0 90-91 23.72470778707945 33.0 2.0 35.0 2.0 35.5 92-93 23.348839045964304 33.0 2.0 35.0 2.0 35.0 94-95 22.9332451429474 33.0 2.0 35.0 2.0 35.0 96-97 22.49794661190965 32.5 2.0 35.0 2.0 35.0 98-99 21.99453087979782 32.0 2.0 35.0 2.0 35.0 100-101 20.541561364713317 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2394692269903267 1101 2 1.6069694608422544 1101 3 1.866051838098354 1101 4 1.5695653598119534 1101 5 1.2441220203656513 1101 6 0.8344935255877459 1101 7 1.031693047821662 1101 8 1.1677067166113204 1101 9 1.386571233866455 1101 10-11 1.5215641143285907 1101 12-13 1.4421438519345102 1101 14-15 1.5987862556075427 1101 16-17 1.57832554320143 1101 18-19 1.3486617710117699 1101 20-21 1.8700583671426116 1101 22-23 1.5941290972836697 1101 24-25 2.135021865286653 1101 26-27 2.2381492679606296 1101 28-29 2.2196713643690558 1101 30-31 2.2789229123795636 1101 32-33 2.1042990426219035 1101 34-35 3.0185307926499547 1101 36-37 2.547034346352312 1101 38-39 2.272410691299058 1101 40-41 3.266207273258935 1101 42-43 3.793749786130128 1101 44-45 4.42404096315331 1101 46-47 4.079227458975733 1101 48-49 4.590875628826719 1101 50-51 4.252519534884502 1101 52-53 4.905308108929859 1101 54-55 5.128248773639722 1101 56-57 5.40991816400393 1101 58-59 5.906779518099881 1101 60-61 6.106132207604865 1101 62-63 6.368678817172523 1101 64-65 6.594043308993953 1101 66-67 6.104263586238126 1101 68-69 5.995756584657979 1101 70-71 5.83588453905336 1101 72-73 5.176634126910635 1101 74-75 5.961072305680769 1101 76-77 6.288445385925328 1101 78-79 6.349459692725297 1101 80-81 5.649341637215297 1101 82-83 6.128295740521072 1101 84-85 6.30127652562096 1101 86-87 6.4980329887411585 1101 88-89 5.977125092797294 1101 90-91 5.947619465860271 1101 92-93 6.134663310663196 1101 94-95 6.511363078419624 1101 96-97 6.8479239643215735 1101 98-99 7.716670880737119 1101 100-101 7.066652414338055 1102 1 0.6495436015677463 1102 2 0.7624799408963447 1102 3 1.0114203303228315 1102 4 0.9191258736726695 1102 5 0.4696122164440837 1102 6 0.735778177379494 1102 7 0.7950466651306556 1102 8 0.5233524231698041 1102 9 0.9734542629401517 1102 10-11 0.9860671569249391 1102 12-13 1.2303453394260586 1102 14-15 1.4875624557427685 1102 16-17 1.3219022842426753 1102 18-19 0.9433203240881696 1102 20-21 0.9055553245462633 1102 22-23 1.2100858248022632 1102 24-25 1.10899076319064 1102 26-27 1.125404169921417 1102 28-29 1.312808619271017 1102 30-31 1.3446768001415634 1102 32-33 2.2227912670978967 1102 34-35 2.6324591226026257 1102 36-37 2.127325083336764 1102 38-39 2.625182834639155 1102 40-41 2.2389929392494707 1102 42-43 3.0040270004641414 1102 44-45 3.2392877515238254 1102 46-47 3.6672937199628954 1102 48-49 4.517177183931519 1102 50-51 5.214994179914928 1102 52-53 5.500811827658723 1102 54-55 4.884841065728967 1102 56-57 5.464515865153363 1102 58-59 5.997381276044436 1102 60-61 6.096159252905743 1102 62-63 6.440010798240806 1102 64-65 6.721325256255611 1102 66-67 6.674243302262468 1102 68-69 6.892308308795915 1102 70-71 6.625607324719354 1102 72-73 6.72548470162328 1102 74-75 6.234398877688886 1102 76-77 6.43702550762918 1102 78-79 6.9319478604061615 1102 80-81 6.72270201584951 1102 82-83 6.003549290216807 1102 84-85 6.642216349826501 1102 86-87 6.137654354528852 1102 88-89 6.303697100910881 1102 90-91 6.008640425968451 1102 92-93 5.138720105795041 1102 94-95 5.733134545627195 1102 96-97 6.182609562698854 1102 98-99 6.4798554649156195 1102 100-101 6.630546937664629 1103 1 0.6803559264977146 1103 2 0.6168216775910231 1103 3 1.3069385376057419 1103 4 0.9751482826362547 1103 5 1.029836306079936 1103 6 0.45846725300974356 1103 7 0.017735740760905117 1103 8 0.9813356164471116 1103 9 0.6821377363295085 1103 10-11 0.5539943277932906 1103 12-13 1.247152062115127 1103 14-15 1.5281787022413695 1103 16-17 1.5536950013295154 1103 18-19 1.5875780271694069 1103 20-21 0.9685805346302985 1103 22-23 1.2731110348862984 1103 24-25 1.9080103710337752 1103 26-27 1.8810064108177684 1103 28-29 2.029195173892866 1103 30-31 2.7760493491611626 1103 32-33 3.455284264296779 1103 34-35 3.0575291506138313 1103 36-37 2.8311065959418045 1103 38-39 2.904594599345039 1103 40-41 2.8930545638993337 1103 42-43 3.4641110340775825 1103 44-45 4.234385790739516 1103 46-47 3.997125652735999 1103 48-49 3.1397262035393574 1103 50-51 2.5021090258533043 1103 52-53 4.209495301048079 1103 54-55 4.365933502703765 1103 56-57 4.00023015086764 1103 58-59 4.175252424503817 1103 60-61 4.774030401365124 1103 62-63 5.301355336055934 1103 64-65 5.404798645611365 1103 66-67 6.4039351790131605 1103 68-69 6.189927356414966 1103 70-71 5.332189957772574 1103 72-73 6.118341844480419 1103 74-75 5.779216804859754 1103 76-77 5.229742594463914 1103 78-79 4.724664947240896 1103 80-81 4.388668402404132 1103 82-83 4.810972259404483 1103 84-85 5.222048282599612 1103 86-87 5.326029704668908 1103 88-89 6.014481414636375 1103 90-91 5.788752470786381 1103 92-93 5.652725708035938 1103 94-95 5.859885245907304 1103 96-97 6.004738414239469 1103 98-99 6.013468910293767 1103 100-101 6.8630399348635045 1104 1 0.3089273550691445 1104 2 0.33110739187673843 1104 3 0.06408139474860164 1104 4 0.13705304454101963 1104 5 -0.2844494082057807 1104 6 0.2965624911049858 1104 7 0.8129738359990029 1104 8 -0.1948548597433586 1104 9 0.48213773632951273 1104 10-11 1.0516133754123373 1104 12-13 0.6281044430675067 1104 14-15 0.8805596546223242 1104 16-17 0.9608378584723667 1104 18-19 0.1875780271694012 1104 20-21 0.6257233917731497 1104 22-23 0.26358722536249246 1104 24-25 1.396105609129016 1104 26-27 0.7881492679606268 1104 28-29 0.5125285072262002 1104 30-31 0.5141445872564034 1104 32-33 0.48385569286820385 1104 34-35 0.14562438870906647 1104 36-37 0.6620589768941869 1104 38-39 -0.6977863530359159 1104 40-41 1.3359117067564696 1104 42-43 1.3188729388394904 1104 44-45 1.9843857907395162 1104 46-47 3.3947447003550515 1104 48-49 4.027821441634604 1104 50-51 3.3687756925199714 1104 52-53 3.666638158190935 1104 54-55 3.9302192169894745 1104 56-57 2.44070634134383 1104 58-59 3.2895381387895384 1104 60-61 3.1216494489841757 1104 62-63 3.906117240817842 1104 64-65 3.309560550373263 1104 66-67 3.737268512346496 1104 68-69 5.163736880224487 1104 70-71 5.553618529201145 1104 72-73 4.635008511147088 1104 74-75 4.426835852478799 1104 76-77 5.6345044992258195 1104 78-79 5.205617328193277 1104 80-81 5.3434303071660345 1104 82-83 4.780019878452105 1104 84-85 5.945857806409133 1104 86-87 6.623648752287956 1104 88-89 5.497814747969706 1104 90-91 2.645895327929235 1104 92-93 3.4479638032740354 1104 94-95 3.824170960193019 1104 96-97 3.166643176144234 1104 98-99 4.513468910293771 1104 100-101 5.058278030101601 1105 1 1.39464164078343 1105 2 1.2856528464221917 1105 3 0.7939515246187305 1105 4 -0.7109989035109336 1105 5 1.0623038385474715 1105 6 1.0147443092868045 1105 7 1.0285582515834193 1105 8 0.7194308545423524 1105 9 1.7016182558100326 1105 10-11 1.462002985801945 1105 12-13 0.6897927547558211 1105 14-15 1.6591310831937491 1105 16-17 1.0179807156152236 1105 18-19 1.2499156895070698 1105 20-21 2.088710404760164 1105 22-23 2.18111969289496 1105 24-25 1.8928588558822668 1105 26-27 1.2154219952333634 1105 28-29 1.9014895461872428 1105 30-31 1.2154432885551074 1105 32-33 1.003985562998075 1105 34-35 0.737182830267507 1105 36-37 0.5854356002708059 1105 38-39 -0.03479934004890595 1105 40-41 0.14630131714609007 1105 42-43 0.07666514663170432 1105 44-45 -2.1292505728968436 1105 46-47 -3.70525529964495 1105 48-49 -4.352698038884878 1105 50-51 -3.8799256061813274 1105 52-53 -3.9982969067441267 1105 54-55 -4.572378185607928 1105 56-57 -4.361241710604219 1105 58-59 -4.269552770301377 1105 60-61 -3.7238050964703717 1105 62-63 -3.715960681260082 1105 64-65 -4.099530358717647 1105 66-67 -3.619874344796358 1105 68-69 -3.266782600294995 1105 70-71 -4.583394457811842 1105 72-73 -6.016939540800962 1105 74-75 -5.689397913754966 1105 76-77 -5.310300695579372 1105 78-79 -5.050226827650878 1105 80-81 -4.544232030496303 1105 82-83 -4.34270739427517 1105 84-85 -3.9197266091752816 1105 86-87 -3.88284475420555 1105 88-89 -3.106730706575746 1105 90-91 -2.9450137629798583 1105 92-93 -2.696841391531162 1105 94-95 -2.406348520326457 1105 96-97 -2.0353048758038206 1105 98-99 -1.7527648559399935 1105 100-101 -1.8988648270412547 1106 1 0.8390860852278728 1106 2 0.49777405854340273 1106 3 -1.0898868592196536 1106 4 0.435465742953717 1106 5 0.6885664648100942 1106 6 1.08545137999387 1106 7 1.1447198677450316 1106 8 0.3305419656534667 1106 9 0.4995980537898248 1106 10-11 0.08574035953932224 1106 12-13 -0.4314193664563035 1106 14-15 -0.396424472361808 1106 16-17 0.32353627117078076 1106 18-19 -0.4243267347353523 1106 20-21 -0.8607845447347842 1106 22-23 -1.0840318222565557 1106 24-25 -0.8697674067439962 1106 26-27 -1.345184065372706 1106 28-29 -1.16921752451983 1106 30-31 -0.6961728730610517 1106 32-33 -0.08439827538576594 1106 34-35 0.9265767696614517 1106 36-37 -0.07365530882010063 1106 38-39 -0.45651651176607544 1106 40-41 0.5478164686612388 1106 42-43 0.3291903991569498 1106 44-45 0.9843857907395162 1106 46-47 1.4614113670217215 1106 48-49 1.2786150924282573 1106 50-51 1.541791565535842 1106 52-53 1.4714000629528456 1106 54-55 1.6119652487355047 1106 56-57 1.7145158651533556 1106 58-59 2.722871472122865 1106 60-61 1.766093893428618 1106 62-63 0.8926251773257761 1106 64-65 1.6428938837065985 1106 66-67 2.769014544092528 1106 68-69 3.336752753240358 1106 70-71 3.272666148248767 1106 72-73 3.669929146067723 1106 74-75 4.497470773113722 1106 76-77 4.265456880178203 1106 78-79 4.732601455177406 1106 80-81 5.05930332303905 1106 82-83 5.152242100674325 1106 84-85 5.4641117746631025 1106 86-87 5.758569387208592 1106 88-89 4.986703636858596 1106 90-91 5.5347842168181245 1106 92-93 6.240027295337526 1106 94-95 5.391631277653339 1106 96-97 5.540452699953754 1106 98-99 6.21188160870647 1106 100-101 7.601135172958745 1107 1 -0.6641818886283346 1107 2 -0.963010255182084 1107 3 -2.008187512814427 1107 4 -1.0024427537783112 1107 5 0.39118084389506436 1107 6 -1.7152022147773707 1107 7 -2.067698432908557 1107 8 -0.9864514983988215 1107 9 -3.477526129216706 1107 10-11 -0.2198151960162349 1107 12-13 -1.25004681743669 1107 14-15 -2.032045387394483 1107 16-17 0.3415101273799337 1107 18-19 -0.4586404602255527 1107 20-21 -0.06503291074785977 1107 22-23 -0.3389337830408792 1107 24-25 0.059971155347504634 1107 26-27 -0.3942036732158414 1107 28-29 -3.0303286356309442 1107 30-31 -2.057283984172166 1107 32-33 -2.1497577525099416 1107 34-35 -3.3871487205346327 1107 36-37 -4.647184720584807 1107 38-39 -4.541483832027517 1107 40-41 -4.68257568820151 1107 42-43 -5.044992607378994 1107 44-45 -6.33914362102519 1107 46-47 -8.293490593762598 1107 48-49 -6.620077718029268 1107 50-51 -6.373241114202717 1107 52-53 -6.185462682145193 1107 54-55 -7.173982463682794 1107 56-57 -7.819797860336838 1107 58-59 -8.639873625916351 1107 60-61 -8.73717408042759 1107 62-63 -9.961949986072916 1107 64-65 -9.396321802567915 1107 66-67 -8.208109638914003 1107 68-69 -8.842985808851143 1107 70-71 -9.266549538025743 1107 72-73 -9.254907455239469 1107 74-75 -9.08913053407582 1107 76-77 -8.798268610017878 1107 78-79 -7.852365865084035 1107 80-81 -7.443964650817158 1107 82-83 -7.614097768606719 1107 84-85 -7.455822865859773 1107 86-87 -8.136855449392716 1107 88-89 -6.902185252030293 1107 90-91 -6.765869377953118 1107 92-93 -5.890691658910839 1107 94-95 -6.159022317117904 1107 96-97 -6.4938610255364395 1107 98-99 -7.578968064496145 1107 100-101 -7.722394238805961 1108 1 -0.47202502588323725 1108 2 0.19777405854340557 1108 3 -0.6121090814418757 1108 4 -0.45342314593517585 1108 5 -1.6558779796343472 1108 6 -1.1701041755616828 1108 7 -0.7108356878105226 1108 8 -0.6139024787909833 1108 9 1.177375831567609 1108 10-11 -0.4531485293495692 1108 12-13 -0.2480860331229664 1108 14-15 -0.49086891680624944 1108 16-17 -0.7486859510514421 1108 18-19 -1.1076600680686894 1108 20-21 -0.9218956558458942 1108 22-23 -1.0506984889232243 1108 24-25 0.22467703770045233 1108 26-27 0.588149267960624 1108 28-29 0.4030046977023929 1108 30-31 -0.02395065083883452 1108 32-33 0.4933795023920169 1108 34-35 1.8599101029947747 1108 36-37 -0.7236553088201063 1108 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-1.2385886329782814 2312 48-49 -0.9436071297939712 2312 50-51 -1.0359862122419337 2312 52-53 -0.5619332703804893 2312 54-55 0.3730763598466176 2312 56-57 0.6978491984866899 2312 58-59 1.056204805456197 2312 60-61 1.5549827823175093 2312 62-63 -0.6129303782297768 2312 64-65 -2.2904394496267386 2312 66-67 -3.786541011463026 2312 68-69 -3.4076916912040858 2312 70-71 -3.027333851751237 2312 72-73 -2.8745152983767213 2312 74-75 -2.496973671330725 2312 76-77 -3.0512097864884637 2312 78-79 -4.28962076704482 2312 80-81 -3.8851411214053932 2312 82-83 -3.1866467882145635 2312 84-85 -2.863666003114677 2312 86-87 -2.619208390569188 2312 88-89 -2.068851918696957 2312 90-91 -2.3874380054040962 2312 92-93 -1.9377504824402507 2312 94-95 -2.6139242779022176 2312 96-97 -3.309547300046244 2312 98-99 -2.8770072801824185 2312 100-101 -2.965531493707921 2313 1 -0.10535835921657011 2313 2 -0.01889260812326299 2313 3 -0.6287757481085414 2313 4 -0.27008981260183873 2313 5 -0.2058779796343515 2313 6 0.09656249110498294 2313 7 0.35583097885614734 2313 8 0.36943085454235103 2313 9 -0.48929083509906235 2313 10-11 -0.2198151960162349 2313 12-13 -0.3147526997896293 2313 14-15 -0.5408689168062537 2313 16-17 0.7679807156152236 2313 18-19 0.9590065985979734 2313 20-21 1.7364376774874373 2313 22-23 0.1243015110767729 2313 24-25 0.4996770377004438 2313 26-27 1.088149267960624 2313 28-29 0.9696713643690558 2313 30-31 -0.2822839841721674 2313 32-33 0.37671283572534975 2313 34-35 0.4099101029947789 2313 36-37 1.4013446911799008 2313 38-39 -1.6620720673216312 2313 40-41 -1.285516864672097 2313 42-43 -1.109698489731933 2313 44-45 -3.8406142092604867 2313 46-47 -4.4802552996449485 2313 48-49 -3.1186071297939684 2313 50-51 -2.1026528789086036 2313 52-53 -2.1619332703804908 2313 54-55 -3.2269236401533803 2313 56-57 -2.902150801513308 2313 58-59 -1.8354618612104687 2313 60-61 -0.428350551015825 2313 62-63 0.020402955103556053 2313 64-65 0.20956055037326138 2313 66-67 -0.3948743447963565 2313 68-69 -1.0076916912040836 2313 70-71 0.014332814915430703 2313 72-73 -1.2828486317100563 2313 74-75 -2.8803070046640578 2313 76-77 -5.876209786488467 2313 78-79 -5.922954100378153 2313 80-81 -5.460141121405396 2313 82-83 -4.694980121547896 2313 84-85 -5.071999336448005 2313 86-87 -4.119208390569188 2313 88-89 -4.652185252030293 2313 90-91 -5.5291046720707655 2313 92-93 -5.362750482440251 2313 94-95 -4.8722576112355505 2313 96-97 -4.07621396671291 2313 98-99 -3.29367394684909 2313 100-101 -2.3238648270412554 2314 1 -1.60535835921657 2314 2 -1.4784164176470718 2314 3 -1.6216328909656852 2314 4 -0.6915183840304095 2314 5 -0.970163693920064 2314 6 -0.7320089374664462 2314 7 -1.1013118782867082 2314 8 -0.851997716886217 2314 9 -1.1750051208133456 2314 10-11 -0.9579104341114757 2314 12-13 -1.1814193664563035 2314 14-15 -1.6622974882348203 2314 16-17 -2.0058288081942948 2314 18-19 -1.555279115687732 2314 20-21 -2.3885623225125627 2314 22-23 -1.250698488923227 2314 24-25 -1.4848467718233636 2314 26-27 -1.2737554939441296 2314 28-29 -0.6969953022976085 2314 30-31 -0.03347446036264046 2314 32-33 -1.4018585928460823 2314 34-35 -1.6210422779575993 2314 36-37 -1.2522267373915312 2314 38-39 -1.2263577816073479 2314 40-41 -3.035516864672097 2314 42-43 -1.6192222992557461 2314 44-45 -0.6822808759271481 2314 46-47 -0.08620768059732953 2314 48-49 -0.7293214155082559 2314 50-51 1.5021090258533043 2314 52-53 1.2571143486671303 2314 54-55 1.4492668360370935 2314 56-57 2.4645158651533627 2314 58-59 2.413347662599058 2314 60-61 2.6073637346984597 2314 62-63 3.563260097960697 2314 64-65 2.619084359897073 2314 66-67 1.2372685123464997 2314 68-69 -0.36959645310884426 2314 70-71 -0.39400051841790074 2314 72-73 -0.13165815551958104 2314 74-75 -0.2922117665688191 2314 76-77 0.03926640398772463 2314 78-79 0.2484744710504181 2314 80-81 0.7339064976422272 2314 82-83 1.0609722594044833 2314 84-85 0.7220482825996122 2314 86-87 -0.40016077152156626 2314 88-89 -0.6640900139350521 2314 90-91 -0.42553324349933774 2314 92-93 0.3193923747026055 2314 94-95 0.062266198288259744 2314 96-97 1.004738414239469 2314 98-99 2.0134689102937706 2314 100-101 2.07732564914922 2315 1 -0.8662279244339608 2315 2 -0.4515013037754372 2315 3 0.6081807736305898 2315 4 -0.29400285608009824 2315 5 0.4397741942786979 2315 6 -0.2599592480254529 2315 7 -0.07025597766559599 2315 8 -1.2370908845880848 2315 9 -0.2371169220555842 2315 10-11 0.14975002137506266 2315 12-13 -0.6451874823983275 2315 14-15 -1.2430428298497276 2315 16-17 -0.09071493655869034 2315 18-19 -0.9496890535759377 2315 20-21 -1.4537797138169068 2315 22-23 -1.6420028367493131 2315 24-25 0.020329211613493214 2315 26-27 0.009888398395411002 2315 28-29 -0.20424167910920943 2315 30-31 0.11662905930609924 2315 32-33 -0.6472002077529169 2315 34-35 0.4555622769078269 2315 36-37 0.26330121291903197 2315 38-39 0.9879279326783674 2315 40-41 0.5514396570670286 2315 42-43 2.8207362928767665 2315 44-45 1.027864051609079 2315 46-47 1.5556142655724408 2315 48-49 1.2737841745538567 2315 50-51 0.9451732080479225 2315 52-53 1.7685015122282053 2315 54-55 1.646989403324877 2315 56-57 2.841327459356254 2315 58-59 2.519972921398228 2315 60-61 2.0825190142015657 2315 62-63 2.833446433364422 2315 64-65 3.72260402863413 2315 66-67 4.249690872594947 2315 68-69 3.8053517870567823 2315 70-71 3.091506727958908 2315 72-73 3.044325281333421 2315 74-75 2.0349103866402913 2315 76-77 2.696616300468058 2315 78-79 3.1553067691870673 2315 80-81 4.288771922072868 2315 82-83 4.506106834973842 2315 84-85 4.233435446160687 2315 86-87 3.5677481311699424 2315 88-89 3.7282495305784025 2315 90-91 2.5806779366248875 2315 92-93 3.0057277784293106 2315 94-95 3.4483945626774926 2315 96-97 3.7324816854610035 2315 98-99 2.7215434444552606 2315 100-101 3.4054829990457023 2316 1 -0.4572102110684213 2316 2 -1.4837074229380747 2316 3 -0.7009979703307643 2316 4 -0.564534257046283 2316 5 -0.10772983148620341 2316 6 -0.6738078792653894 2316 7 -0.688613465588297 2316 8 -0.31760618249468564 2316 9 -0.03743898324720618 2316 10-11 -1.9975929737940135 2316 12-13 -1.2554934405303726 2316 14-15 -1.664942990880327 2316 16-17 -2.0005378029032954 2316 18-19 -1.2854378458464666 2316 20-21 -0.499673433623677 2316 22-23 -1.19514293336767 2316 24-25 -1.8419896289662248 2316 26-27 -2.197035917224561 2316 28-29 -1.474773080075387 2316 30-31 -0.6128395397277231 2316 32-33 -1.121435312422804 2316 34-35 -1.0919417488570673 2316 36-37 -0.11995160511639114 2316 38-39 -0.04910910435867066 2316 40-41 -1.109590938746173 2316 42-43 -0.49488367491712637 2316 44-45 -0.8859845796308541 2316 46-47 -2.70525529964495 2316 48-49 -2.054718240905082 2316 50-51 -2.569319545575265 2316 52-53 -3.6952666037138258 2316 54-55 -2.9343310475607893 2316 56-57 -1.7206693200318277 2316 58-59 -1.4993507500993566 2316 60-61 -1.5857579584232333 2316 62-63 -1.5240414893408882 2316 64-65 -2.3941431533304396 2316 66-67 -2.2495039744259877 2316 68-69 -1.8669509504633446 2316 70-71 -1.8384449628623472 2316 72-73 -2.793033816895239 2316 74-75 -2.678455152812205 2316 76-77 -3.4104690457477247 2316 78-79 -2.9711022485263 2316 80-81 -3.19995593622021 2316 82-83 -2.8755356771034535 2316 84-85 -3.4710734105220844 2316 86-87 -4.20439357575437 2316 88-89 -3.420703770548812 2316 90-91 -3.428178746144841 2316 92-93 -4.20441714910692 2316 94-95 -4.691702055679993 2316 96-97 -5.339176929675872 2316 98-99 -5.547377650552791 2316 100-101 -4.5470129751894035 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 205.0 3 48.0 4 43.0 5 40.0 6 42.0 7 34.0 8 48.0 9 77.0 10 86.0 11 159.0 12 191.0 13 236.0 14 306.0 15 435.0 16 576.0 17 705.0 18 572.0 19 363.0 20 336.0 21 354.0 22 338.0 23 371.0 24 376.0 25 367.0 26 388.0 27 362.0 28 385.0 29 394.0 30 505.0 31 588.0 32 681.0 33 854.0 34 1210.0 35 2196.0 36 2219.0 37 3697.0 38 4566.0 39 971.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.45789224206036 24.707694738505293 19.292147258650655 17.542265760783692 2 14.40252814536836 25.281453683586808 40.72289156626506 19.593126604779776 3 15.111778181530926 33.03183505806146 35.2515996524212 16.604787107986414 4 9.315739570164348 24.16245259165613 40.41956384323641 26.10224399494311 5 8.231249506045996 39.725756737532606 38.28736268078716 13.755631075634236 6 19.932045355774168 43.13934652917703 20.777527557188574 16.15108055786022 7 17.43322269638059 40.88825667773036 26.2604709973131 15.418049628575945 8 20.11378452056418 35.59322033898305 24.17130891707163 20.12168622338114 9 22.19017895942796 20.28601904159918 25.504681389009598 32.01912060996326 10-11 18.924739681096995 30.985358914070066 29.475805654897158 20.614095749935785 12-13 22.369512821525937 31.542139159982614 29.872772531510527 16.215575486980917 14-15 19.41410031013571 33.01068684194931 30.70541058412184 16.869802263793137 16-17 13.775802469135803 36.23506172839506 31.879506172839505 18.109629629629627 18-19 13.088819102921134 34.11151270960479 37.151152456005214 15.648515731468862 20-21 14.487458028836658 33.06735137270393 37.16176180130357 15.283428797155837 22-23 14.458093056323564 32.58748716328304 37.05268978592306 15.901729994470337 24-25 13.872158922612114 33.58346004225825 37.372879682470725 15.171501352658911 26-27 13.911652613494994 33.575561304081674 37.68487984044549 14.827906241977844 28-29 13.084259789498628 33.45510554688889 38.464880235382395 14.99575442823009 30-31 13.423640456538052 34.06855969353501 37.429406421547334 15.078393428379606 32-33 13.046225539561243 34.87155184329522 37.20553679679324 14.876685820350295 34-35 13.695871097683787 33.95928361274016 36.95476176371858 15.390083525857474 36-37 13.94044704209778 34.04944317194534 36.44854276913356 15.561567016823316 38-39 13.744964852697258 34.59244925361346 36.543322012479265 15.119263881210015 40-41 12.84704395560997 34.38252833616366 37.47482326922317 15.295604439003199 42-43 13.502093041623883 34.4502803885949 36.23134033646631 15.816286233314905 44-45 13.671096017533122 34.132327679823085 36.09888048650463 16.09769581613916 46-47 14.131422027405915 33.36887414603325 35.712593294633336 16.787110531927496 48-49 14.300663402306114 33.241194124151 35.655899541936506 16.802242931606383 50-51 14.573995458584262 32.816665021226186 35.614572020930005 16.994767499259552 52-53 14.53281731300845 33.7493089013506 34.815575388989814 16.902298396651133 54-55 14.334518167456556 32.804107424960506 35.294233807266984 17.567140600315955 56-57 14.524091627172195 33.56437598736177 34.385860979462876 17.52567140600316 58-59 14.009833540667021 32.07353434828111 35.65152143435421 18.265110676697667 60-61 13.839409139381493 32.73233540029227 35.3015521940045 18.126703266321734 62-63 14.012361525246341 31.739104678027687 36.15844868782212 18.090085108903853 64-65 14.237475563278768 31.23161075018266 36.146600580557255 18.38431310598132 66-67 13.973695643587819 31.428966388877917 35.21861052964177 19.378727437892493 68-69 14.078638149969391 31.034618954519424 35.43456365898454 19.45217923652665 70-71 14.705766192733016 30.32385466034755 35.444312796208536 19.5260663507109 72-73 14.568110904854063 29.558829337651567 35.17121529286307 20.701844464631307 74-75 14.262016667324934 29.797780323077532 35.236383743433784 20.70381926616375 76-77 14.836683913266716 29.4916860855484 35.0310043840594 20.64062561712548 78-79 14.393206280240939 29.09055001481189 35.05875382640466 21.45748987854251 80-81 14.690314425659661 29.362853531363562 33.964686364354556 21.982145678622214 82-83 14.530589675737588 28.494411311663175 34.16999091591295 22.805008096686283 84-85 14.692523401398159 27.787432363047515 33.94683834274655 23.57320589280777 86-87 15.009084445848803 28.43036574768939 33.709218737657004 22.851331068804804 88-89 15.596674499891389 27.69012026303837 33.397183988625365 23.316021248444873 90-91 15.138230647709323 26.962875197472354 34.304897314375985 23.593996840442337 92-93 15.353319903622072 27.83702650393017 33.54465378994352 23.264999802504246 94-95 15.09825219709687 27.573812580231067 33.089760047398045 24.23817517527402 96-97 15.283730668194117 27.25908076398902 32.542613916925085 24.914574650891783 98-99 16.268320625765416 27.144155177181684 32.056650732825034 24.530873464227867 100-101 16.785523794472653 26.49098200351633 31.943264653009624 24.780229549001405 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 799.0 1 482.5 2 173.0 3 157.0 4 107.0 5 88.5 6 147.5 7 297.5 8 403.0 9 360.5 10 250.5 11 165.0 12 143.0 13 129.0 14 124.5 15 122.0 16 114.0 17 127.5 18 115.5 19 113.5 20 127.5 21 138.5 22 163.5 23 168.5 24 173.0 25 208.5 26 262.0 27 309.0 28 314.0 29 373.0 30 470.5 31 544.0 32 697.0 33 835.5 34 948.0 35 1051.0 36 1174.0 37 1298.0 38 1240.0 39 1132.5 40 1030.5 41 887.5 42 790.5 43 745.0 44 675.0 45 646.5 46 593.0 47 555.0 48 802.0 49 804.0 50 524.5 51 352.5 52 207.5 53 145.5 54 128.0 55 116.0 56 101.5 57 88.0 58 85.5 59 76.5 60 59.5 61 55.0 62 50.5 63 38.0 64 29.0 65 21.0 66 16.5 67 14.0 68 9.5 69 5.0 70 3.0 71 4.5 72 4.0 73 2.5 74 1.5 75 2.0 76 1.5 77 0.0 78 0.5 79 1.0 80 0.5 81 0.5 82 0.5 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.031590586005370395 2 0.035539409256041696 3 0.023692939504027802 4 0.047385879008055604 5 0.07107881851208339 6 0.051334702258726904 7 0.06318117201074079 8 0.051334702258726904 9 0.0434370557573843 10-11 0.07305323013741905 12-13 0.059232348760069505 14-15 0.04936029063339126 16-17 0.04541146738271995 18-19 0.03356499763070605 20-21 0.035539409256041696 22-23 0.023692939504027802 24-25 0.01382088137734955 26-27 0.01382088137734955 28-29 0.01382088137734955 30-31 0.011846469752013901 32-33 0.00987205812667825 34-35 0.00987205812667825 36-37 0.007897646501342599 38-39 0.007897646501342599 40-41 0.011846469752013901 42-43 0.007897646501342599 44-45 0.0019744116253356497 46-47 0.003948823250671299 48-49 0.0 50-51 0.0059232348760069505 52-53 0.007897646501342599 54-55 0.015795293002685198 56-57 0.015795293002685198 58-59 0.00987205812667825 60-61 0.0197441162533565 62-63 0.01382088137734955 64-65 0.01382088137734955 66-67 0.0197441162533565 68-69 0.02171852787869215 70-71 0.015795293002685198 72-73 0.0197441162533565 74-75 0.0197441162533565 76-77 0.0197441162533565 78-79 0.025667351129363452 80-81 0.031590586005370395 82-83 0.0197441162533565 84-85 0.0197441162533565 86-87 0.023692939504027802 88-89 0.017769704628020848 90-91 0.015795293002685198 92-93 0.0276417627546991 94-95 0.025667351129363452 96-97 0.03751382088137735 98-99 0.0434370557573843 100-101 0.05330911388406255 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 25324.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.60906649818354 #Duplication Level Percentage of deduplicated Percentage of total 1 76.5024277640691 37.95214026220186 2 10.68216190400382 10.598641604801768 3 4.79980896282735 7.143421260464382 4 2.348165247154342 4.659611435792134 5 1.5044177346175276 3.731637971884379 6 1.19398232906153 3.55394092560417 7 0.581071400143278 2.0178486810930343 8 0.4537132850433814 1.8006634023061125 9 0.2706359945872801 1.2083399147054177 >10 1.5442171455862452 11.913599747275311 >50 0.06367905754994826 2.2429316063812985 >100 0.03183952877497413 3.976465013425999 >500 0.0238796465812306 9.200758174064129 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 909 3.5894803348602116 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 892 3.5223503395987996 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 529 2.0889274996051177 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 396 1.5637340072658348 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 306 1.2083399147054177 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 196 0.7739693571315748 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 109 0.4304217343231717 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 97 0.3830358553151161 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 80 0.315905860053704 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 75 0.2961617438003475 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 74 0.2922129205496762 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 73 0.2882640972990049 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 59 0.2329805717896067 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 59 0.2329805717896067 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 51 0.2013899857842363 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 47 0.1855946927815511 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 45 0.1776970462802085 No Hit GTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 44 0.1737482230295372 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 43 0.1697993997788659 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 41 0.1619017532775233 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 39 0.1540041067761807 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 39 0.1540041067761807 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38 0.1500552835255094 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 37 0.1461064602748381 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 34 0.1342599905228242 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 32 0.12636234402148158 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 28 0.1105670510187964 No Hit TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 28 0.1105670510187964 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 28 0.1105670510187964 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 27 0.1066182277681251 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 26 0.10266940451745381 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26 0.10266940451745381 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 26 0.10266940451745381 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.00197441162533565 0.0 22-23 0.0 0.0 0.0 0.0118464697520139 0.0 24-25 0.0 0.0 0.0 0.0315905860053704 0.0 26-27 0.0 0.0 0.0 0.0552835255093982 0.0 28-29 0.0 0.0 0.0 0.12833675564681726 0.0 30-31 0.0 0.0 0.0 0.19151792765755804 0.0 32-33 0.0 0.0 0.0 0.2487758647922919 0.0 34-35 0.0 0.0 0.0 0.28431527404833357 0.0 36-37 0.0 0.0 0.0 0.3297267414310535 0.0 38-39 0.0 0.0 0.0 0.4146264413204865 0.0 40-41 0.0 0.0 0.0 0.5607329015953246 0.0 42-43 0.0 0.0 0.0 0.7660717106302322 0.0 44-45 0.0 0.0 0.0 0.9970778707945033 0.0 46-47 0.0 0.0 0.0 1.4413204864950244 0.0 48-49 0.0 0.0 0.0 1.810535460432791 0.0 50-51 0.0 0.0 0.0 2.108671615858474 0.0 52-53 0.0 0.0 0.0 2.36929395040278 0.0 54-55 0.0 0.0 0.0 2.6259674616964146 0.0 56-57 0.0 0.0 0.0 3.170905070289054 0.0 58-59 0.0 0.0 0.0 3.617122097614911 0.0 60-61 0.0 0.0 0.0 4.063339124940768 0.0 62-63 0.0 0.0 0.0 4.472042331385247 0.0 64-65 0.0 0.0 0.0 4.918259358711104 0.0 66-67 0.0 0.0 0.0 5.548096667193176 0.0 68-69 0.0 0.0 0.0 6.278628968567366 0.0 70-71 0.0 0.0 0.0 6.874901279418733 0.0 72-73 0.0 0.0 0.0 7.368504185752646 0.0 74-75 0.0 0.0 0.0 8.00229031748539 0.0 76-77 0.0 0.0 0.0 8.596588216711421 0.0 78-79 0.0 0.0 0.0 9.271836992576212 0.0 80-81 0.0 0.0 0.0 10.166245458853261 0.0 82-83 0.0 0.0 0.0 11.015242457747592 0.0 84-85 0.0 0.0 0.0 11.93729268677934 0.0 86-87 0.0 0.0 0.0 12.738903806665615 0.0 88-89 0.0 0.0 0.0 13.702416679829412 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACCCTC 25 0.0049337256 57.090298 7 CCCTCTA 25 0.0049337256 57.090298 9 CGCAGAA 45 9.3737297E-4 42.289112 9 TCGGTGG 30 8.102681E-5 39.64604 94-95 GGTATCA 380 0.0 38.888393 1 ATCTCGG 35 2.0187421E-4 33.948704 92-93 TCTACTA 30 0.0039232527 31.65415 16-17 CTCGGTG 40 4.3928303E-4 29.73453 94-95 GTATCAA 520 0.0 28.41844 2 AGATCTC 45 8.8498107E-4 26.37846 90-91 AACGCAG 565 0.0 26.103235 7 CAGAAAA 55 9.202372E-5 25.950134 9 TCAACGC 570 0.0 25.925594 5 GTACTTT 360 0.0 25.718998 14-15 GAGTACT 360 0.0 25.718998 12-13 ATCAACG 575 0.0 25.700155 4 CAACGCA 580 0.0 25.42815 6 TATCAAC 565 0.0 25.311316 3 ACGCAGA 590 0.0 24.997164 8 TGGTATC 220 1.8462742E-9 23.834822 2 >>END_MODULE