##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936976_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29176 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.690773238278037 33.0 31.0 34.0 30.0 34.0 2 31.764429668220455 33.0 31.0 34.0 30.0 34.0 3 31.685220729366602 33.0 31.0 34.0 30.0 34.0 4 35.30538799012887 37.0 35.0 37.0 33.0 37.0 5 35.30134357005758 37.0 35.0 37.0 33.0 37.0 6 35.4179462571977 37.0 35.0 37.0 33.0 37.0 7 35.398066904304905 37.0 35.0 37.0 33.0 37.0 8 35.381238003838774 37.0 35.0 37.0 33.0 37.0 9 37.00281052920209 39.0 37.0 39.0 33.0 39.0 10-11 36.87899300795174 39.0 37.0 39.0 33.0 39.0 12-13 36.83578969015629 39.0 37.0 39.0 33.0 39.0 14-15 37.93924801206471 40.0 38.0 41.0 33.0 41.0 16-17 37.82147998354812 40.0 37.5 41.0 33.0 41.0 18-19 37.65805799287085 40.0 37.0 41.0 33.0 41.0 20-21 37.630244036194135 40.0 37.0 41.0 33.0 41.0 22-23 37.480394845078145 40.0 36.0 41.0 33.0 41.0 24-25 37.38416849465314 40.0 36.0 41.0 33.0 41.0 26-27 37.202118179325474 40.0 35.5 41.0 33.0 41.0 28-29 36.94538319166438 39.0 35.0 41.0 32.0 41.0 30-31 36.61593432958596 39.0 35.0 41.0 32.0 41.0 32-33 36.186488894982176 38.0 35.0 41.0 31.0 41.0 34-35 35.87297778996435 38.0 35.0 41.0 31.0 41.0 36-37 35.58212229229504 38.0 35.0 40.5 30.0 41.0 38-39 35.3420962434878 38.0 35.0 40.0 30.0 41.0 40-41 35.00916849465314 38.0 35.0 40.0 28.0 41.0 42-43 34.58674938305457 38.0 35.0 40.0 25.0 41.0 44-45 34.040632711817935 38.0 35.0 40.0 21.5 41.0 46-47 33.37455442829723 38.0 34.5 40.0 7.5 41.0 48-49 32.736547162051 38.0 33.5 40.0 2.0 41.0 50-51 31.82890046613655 37.0 32.0 39.5 2.0 40.5 52-53 31.898478201261312 37.0 33.0 39.5 2.0 40.5 54-55 32.14014943789416 38.0 33.0 40.0 2.0 41.0 56-57 31.88651631477927 38.0 33.0 40.0 2.0 41.0 58-59 31.567744036194135 37.5 33.0 40.0 2.0 41.0 60-61 31.197765286536878 37.0 32.0 40.0 2.0 41.0 62-63 30.8018405538799 36.5 31.0 40.0 2.0 41.0 64-65 30.38557375925418 36.0 31.0 40.0 2.0 41.0 66-67 29.971226350425006 36.0 30.0 39.0 2.0 41.0 68-69 29.495647107211404 35.0 29.0 39.0 2.0 41.0 70-71 29.000565533315054 35.0 28.0 39.0 2.0 40.5 72-73 28.5275226213326 35.0 26.5 38.0 2.0 40.0 74-75 28.040392788593365 35.0 26.0 37.0 2.0 39.5 76-77 27.55555936386071 35.0 24.5 37.0 2.0 39.0 78-79 27.071737044145873 35.0 22.5 36.0 2.0 39.0 80-81 26.545019879352893 34.0 19.0 36.0 2.0 37.5 82-83 26.108736632848917 34.0 13.5 35.0 2.0 37.0 84-85 25.65535028790787 34.0 4.5 35.0 2.0 37.0 86-87 25.27635727995613 34.0 2.0 35.0 2.0 36.0 88-89 24.832653550863725 34.0 2.0 35.0 2.0 36.0 90-91 24.363466547847544 33.0 2.0 35.0 2.0 35.5 92-93 23.98987181244859 33.0 2.0 35.0 2.0 35.0 94-95 23.553194406361392 33.0 2.0 35.0 2.0 35.0 96-97 23.13942966822046 33.0 2.0 35.0 2.0 35.0 98-99 22.698587880449686 32.5 2.0 35.0 2.0 35.0 100-101 21.112609679188374 29.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8096448693788538 1101 2 0.8215814695552588 1101 3 0.9797858113524924 1101 4 0.9909580504069098 1101 5 0.9181717715976134 1101 6 1.0035108692462913 1101 7 0.7914019791809039 1101 8 1.0084256316767792 1101 9 0.8074587302199845 1101 10-11 0.7463194409506642 1101 12-13 0.8548429783653333 1101 14-15 1.088102235978809 1101 16-17 0.8877425562012249 1101 18-19 1.1739101245032302 1101 20-21 1.4020502583609442 1101 22-23 1.3521886779592052 1101 24-25 1.5132802460144177 1101 26-27 1.5542269296001763 1101 28-29 1.8549015092844456 1101 30-31 1.734502077441654 1101 32-33 2.3021252620030666 1101 34-35 2.1978381172796944 1101 36-37 2.7298437414674694 1101 38-39 3.1796056285008376 1101 40-41 3.434421526423911 1101 42-43 3.6443233163037263 1101 44-45 3.887760103783812 1101 46-47 4.616795097331426 1101 48-49 4.937764817226139 1101 50-51 4.814928107708326 1101 52-53 5.594797383752596 1101 54-55 5.747694670027851 1101 56-57 6.653026604358125 1101 58-59 6.563924992575526 1101 60-61 6.5115955385379145 1101 62-63 6.850613222258488 1101 64-65 7.143199482769461 1101 66-67 7.246737927877362 1101 68-69 7.018860823514146 1101 70-71 6.482426211897941 1101 72-73 6.878493711368709 1101 74-75 5.732061880060879 1101 76-77 6.34758238977086 1101 78-79 6.9015231976779745 1101 80-81 6.581214395759492 1101 82-83 7.099050638970798 1101 84-85 6.714454443024586 1101 86-87 7.287870743710506 1101 88-89 7.574142035947823 1101 90-91 7.749380988076258 1101 92-93 7.802759501889696 1101 94-95 8.213649575999934 1101 96-97 8.027667881104726 1101 98-99 8.319319805674592 1101 100-101 7.160521320714281 1102 1 0.25105349111534636 1102 2 -0.057592786668177354 1102 3 0.29642211979207644 1102 4 -0.25980090527007604 1102 5 0.25788033261764554 1102 6 0.19992859845090294 1102 7 0.1945592347971825 1102 8 0.07096357946063137 1102 9 0.43951701801597665 1102 10-11 -0.028574305254558396 1102 12-13 0.164345103441228 1102 14-15 -0.39110237847170026 1102 16-17 0.5263278628193078 1102 18-19 0.07661200671330448 1102 20-21 -0.46604324497845795 1102 22-23 -0.6403735564063098 1102 24-25 -0.6467076106947545 1102 26-27 -0.16511126104948914 1102 28-29 0.15666228645505953 1102 30-31 0.4111869590445707 1102 32-33 0.9674258084511465 1102 34-35 1.8951665932967003 1102 36-37 1.6928067044304314 1102 38-39 1.1225321615913089 1102 40-41 1.099722072871998 1102 42-43 1.4796299343972308 1102 44-45 1.7667825688718537 1102 46-47 2.5251132515512253 1102 48-49 3.0643586241478147 1102 50-51 2.988577166603278 1102 52-53 3.4779182094963694 1102 54-55 3.468854354302291 1102 56-57 4.088515371814104 1102 58-59 3.0079140636137787 1102 60-61 3.5246495761821244 1102 62-63 3.568888874960372 1102 64-65 3.7166663923019456 1102 66-67 4.448923720227086 1102 68-69 4.084131011735153 1102 70-71 4.277659970246457 1102 72-73 3.4244864253942104 1102 74-75 4.076931339684442 1102 76-77 3.9266655713130554 1102 78-79 3.916095146676156 1102 80-81 3.6818519185281637 1102 82-83 4.769815666293201 1102 84-85 4.526081643996051 1102 86-87 4.959091144439103 1102 88-89 3.83947294062299 1102 90-91 3.905270484129691 1102 92-93 5.286214268131346 1102 94-95 5.370601610001149 1102 96-97 5.150770491912255 1102 98-99 6.001317377016427 1102 100-101 4.365439353501166 1103 1 1.0038715265420812 1103 2 0.9609257318503417 1103 3 0.4502063387936879 1103 4 0.7732103668716306 1103 5 0.7385566611200645 1103 6 1.0566113681771512 1103 7 1.0850584296441994 1103 8 1.0073564941144184 1103 9 1.4564252305763645 1103 10-11 1.5563693340369085 1103 12-13 1.0806091936183648 1103 14-15 1.0783018083237863 1103 16-17 1.113686961047975 1103 18-19 1.1977875300627403 1103 20-21 1.2835541785320075 1103 22-23 1.2730724983440922 1103 24-25 1.8251120350379395 1103 26-27 2.092537692251639 1103 28-29 2.3313804829123796 1103 30-31 1.8544236740204099 1103 32-33 1.8949620403352085 1103 34-35 2.0187575755833294 1103 36-37 2.1320176545109533 1103 38-39 2.689762435343326 1103 40-41 2.4837800438864903 1103 42-43 2.8278585978432815 1103 44-45 1.5787793482599355 1103 46-47 2.580266230617248 1103 48-49 2.0450349526502265 1103 50-51 1.7160328831894276 1103 52-53 2.51817585844967 1103 54-55 2.9298044348175942 1103 56-57 3.0901256777722352 1103 58-59 3.4028415998456616 1103 60-61 2.6438122170838874 1103 62-63 2.6139774417880695 1103 64-65 2.4537839446368928 1103 66-67 2.267764299937234 1103 68-69 2.864726824939666 1103 70-71 3.2297533679920285 1103 72-73 3.2131337683893797 1103 74-75 3.0374788437102076 1103 76-77 3.3912388402341485 1103 78-79 3.469235243294513 1103 80-81 2.6371659281899973 1103 82-83 3.244453347452623 1103 84-85 2.9999141721764033 1103 86-87 3.5508785840526293 1103 88-89 4.011775678143117 1103 90-91 4.455189968831782 1103 92-93 4.149338261690122 1103 94-95 3.780827052835285 1103 96-97 4.048516063570869 1103 98-99 3.343909969609019 1103 100-101 4.017613266544643 1104 1 0.42539856789944963 1104 2 0.7048281708747304 1104 3 0.7810652995572056 1104 4 1.1448964750370578 1104 5 1.18606461446047 1104 6 0.9203441359396152 1104 7 1.2144327668658335 1104 8 1.3482896860550326 1104 9 0.9490021128669284 1104 10-11 1.6003778176000054 1104 12-13 1.0543631702885605 1104 14-15 1.187262571844471 1104 16-17 0.8029764626386466 1104 18-19 1.2624746986735573 1104 20-21 1.4646252283729382 1104 22-23 1.7094457751203365 1104 24-25 0.9756952800008207 1104 26-27 0.9692609160056165 1104 28-29 1.234349729996154 1104 30-31 1.2269051162261349 1104 32-33 2.0060436946300158 1104 34-35 2.161652591490011 1104 36-37 1.2664291073211231 1104 38-39 1.6460190631269853 1104 40-41 2.3090716663679345 1104 42-43 1.6953029244604636 1104 44-45 2.5962766971464717 1104 46-47 2.249142158506963 1104 48-49 1.468152662088201 1104 50-51 0.6547921620865722 1104 52-53 1.8500952646002595 1104 54-55 1.7071109035344527 1104 56-57 1.3353536735304559 1104 58-59 2.3148246327194677 1104 60-61 2.4980009763627855 1104 62-63 2.3340198596035506 1104 64-65 2.485862417595534 1104 66-67 2.957053801527902 1104 68-69 2.7313758175165432 1104 70-71 2.6003790307703945 1104 72-73 2.3425081056110137 1104 74-75 2.9165880695850745 1104 76-77 3.6852473237972454 1104 78-79 3.470295688681574 1104 80-81 2.656784167850656 1104 82-83 1.914124609382636 1104 84-85 2.031727533788281 1104 86-87 2.6325328788564484 1104 88-89 2.232878541345663 1104 90-91 1.3621358861170414 1104 92-93 2.3049586222415535 1104 94-95 2.1718662893146075 1104 96-97 2.668611503655704 1104 98-99 1.917080701316337 1104 100-101 1.231293012037753 1105 1 0.547349787411644 1105 2 1.090555361479968 1105 3 -0.5739482505782938 1105 4 0.36982872435955016 1105 5 0.5726951474324622 1105 6 0.5147434132657196 1105 7 -0.7684037281657865 1105 8 0.44133394983099805 1105 9 0.18025775875671712 1105 10-11 0.03624050956025826 1105 12-13 0.5439747330708613 1105 14-15 0.7292679918986664 1105 16-17 -0.18663510014365414 1105 18-19 0.43772311782441875 1105 20-21 0.3580308290956111 1105 22-23 0.12814496211220217 1105 24-25 1.4829220189348788 1105 26-27 1.177481331543106 1105 28-29 1.6196252494180214 1105 30-31 1.5037795516371588 1105 32-33 0.5692776603030083 1105 34-35 0.8396110377411432 1105 36-37 2.581695593319317 1105 38-39 2.8817914208505613 1105 40-41 3.386759109909029 1105 42-43 4.1185188232861165 1105 44-45 4.535301087390373 1105 46-47 4.284372510810478 1105 48-49 3.212506772295967 1105 50-51 3.414503092529202 1105 52-53 4.144584876163041 1105 54-55 4.774409909857848 1105 56-57 5.134811668110402 1105 58-59 4.646802952502664 1105 60-61 5.006131057663605 1105 62-63 4.550370356441853 1105 64-65 5.920370096005655 1105 66-67 5.041516312819681 1105 68-69 4.0656124932166335 1105 70-71 4.360993303579789 1105 72-73 4.767079017986806 1105 74-75 5.3547091174622174 1105 76-77 5.176665571313055 1105 78-79 6.508687739268751 1105 80-81 6.135555622231866 1105 82-83 5.223519369996907 1105 84-85 4.720526088440494 1105 86-87 5.199831885179844 1105 88-89 5.765398866548914 1105 90-91 7.2478630767222825 1105 92-93 6.96214019405727 1105 94-95 7.870601610001149 1105 96-97 7.197066788208549 1105 98-99 6.899465525164576 1105 100-101 6.309883797945609 1106 1 1.2864802221942533 1106 2 1.200056166632951 1106 3 1.319771556184989 1106 4 0.6645147146977166 1106 5 0.9124697045983226 1106 6 1.3392200638293303 1106 7 0.9111453861659413 1106 8 0.24648692889702062 1106 9 0.6085991436198412 1106 10-11 1.0998475949064712 1106 12-13 1.5480004979661928 1106 14-15 1.8500409387585748 1106 16-17 1.9615130480044982 1106 18-19 2.002135356149701 1106 20-21 1.7074672220102656 1106 22-23 1.2295942374745295 1106 24-25 1.520764208950979 1106 26-27 2.2338420400777252 1106 28-29 2.527032656825426 1106 30-31 1.8000758479334564 1106 32-33 2.0362663881612946 1106 34-35 0.5078880103659387 1106 36-37 1.642887219728344 1106 38-39 1.8310667831694047 1106 40-41 2.103345261277795 1106 42-43 2.2191629456693676 1106 44-45 3.1439967395642867 1106 46-47 1.4172227523563734 1106 48-49 2.4146001700415383 1106 50-51 0.20516331797204046 1106 52-53 1.0399149888844548 1106 54-55 1.2776305217741104 1106 56-57 0.8401256777722352 1106 58-59 1.6854502954978337 1106 60-61 2.4264209127360594 1106 62-63 2.6357165722228544 1106 64-65 2.9646535098542834 1106 66-67 3.4851556042850618 1106 68-69 3.4625529118961857 1106 70-71 3.6645359766876773 1106 72-73 3.0174815944763367 1106 74-75 3.5157397132754262 1106 76-77 3.728195361973281 1106 78-79 3.317061330251036 1106 80-81 1.7023833194943485 1106 82-83 3.070540303974365 1106 84-85 3.586870693915536 1106 86-87 4.181313366661325 1106 88-89 4.337862634664859 1106 90-91 3.53127692535352 1106 92-93 2.268903479081427 1106 94-95 3.9221314006613746 1106 96-97 3.7550378027013025 1106 98-99 4.235214317435105 1106 100-101 2.8654393535011664 1107 1 -0.8415391014772453 1107 2 -0.8168520459274369 1107 3 -0.7406149172449616 1107 4 -0.741282386751557 1107 5 -0.6865641118267973 1107 6 -2.6704417719194637 1107 7 -1.6758111355731913 1107 8 -2.4105179020208496 1107 9 -2.5604829819840234 1107 10-11 -2.3156113422915965 1107 12-13 -1.854173415077291 1107 14-15 -1.492954230323555 1107 16-17 -1.5662647297732804 1107 18-19 -1.5993139192126264 1107 20-21 -0.5678950968303127 1107 22-23 -0.010743926776683566 1107 24-25 0.029218315231169356 1107 26-27 0.32562947969125133 1107 28-29 0.6103659901587619 1107 30-31 0.21674251460012783 1107 32-33 -2.0325741915488535 1107 34-35 -1.8918704437403377 1107 36-37 -1.686822925199202 1107 38-39 -0.784875245816103 1107 40-41 -0.28916681601689476 1107 42-43 0.16481511958241413 1107 44-45 -1.4924766903874058 1107 46-47 -2.5211830447450723 1107 48-49 -1.6300858202966282 1107 50-51 -1.8262376482115315 1107 52-53 -1.0035632719851115 1107 54-55 -0.994108608660671 1107 56-57 -2.6707438874451555 1107 58-59 -2.075419269719557 1107 60-61 -1.5772022756697304 1107 62-63 -3.301481495410002 1107 64-65 -4.014815089179535 1107 66-67 -2.8566318353284643 1107 68-69 -2.5640171364129927 1107 70-71 -2.676043733457245 1107 72-73 -2.3255135746057896 1107 74-75 -2.293439030685928 1107 76-77 -2.8696307249832422 1107 78-79 -2.0005715199905154 1107 80-81 -2.595925859249615 1107 82-83 -2.1190732225956843 1107 84-85 -4.594288726374323 1107 86-87 -5.42979774444979 1107 88-89 -5.679045577895529 1107 90-91 -6.159544330685126 1107 92-93 -5.510082028164952 1107 94-95 -3.5368057974062594 1107 96-97 -3.802933211791448 1107 98-99 -3.2949789192798704 1107 100-101 -2.0790050909432765 1108 1 -0.3526502125883546 1108 2 -0.2390742681496576 1108 3 0.5371628605328169 1108 4 -0.22461572008489128 1108 5 -0.33100855627124304 1108 6 1.0740026725249763 1108 7 1.0741888644268087 1108 8 1.2117043202013704 1108 9 0.5172947957937524 1108 10-11 0.9704997688195149 1108 12-13 0.09582658492271179 1108 14-15 1.7348235474542264 1108 16-17 1.0115130480044954 1108 18-19 0.7173527474540435 1108 20-21 0.573771569836353 1108 22-23 1.1698116287788736 1108 24-25 -0.13467057365772206 1108 26-27 0.12840725746903559 1108 28-29 -0.0729673431745681 1108 30-31 1.1250758479334593 1108 32-33 0.6340924751178179 1108 34-35 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-4.943160136747949 2312 78-79 -5.433578055938224 2312 80-81 -4.97664481349798 2312 82-83 -4.558615706255814 2312 84-85 -5.143308334217458 2312 86-87 -5.171627809809262 2312 88-89 -4.1365619177648085 2312 90-91 -4.734707729377934 2312 92-93 -4.073807518361029 2312 94-95 -6.249224098059855 2312 96-97 -5.989207721595372 2312 98-99 -5.214913559802746 2312 100-101 -4.134560646498834 2313 1 -3.0526502125883574 2313 2 -2.339074268149659 2313 3 -2.8128371394671845 2313 4 -2.12461572008489 2313 5 -1.2310085562712416 2313 6 -2.8259973274750223 2313 7 -2.125811135573194 2313 8 -1.638295679798631 2313 9 -2.9827052042062476 2313 10-11 -3.5045002311804794 2313 12-13 -2.904173415077288 2313 14-15 -2.8401764525457764 2313 16-17 -2.163486951995502 2313 18-19 -2.6576472525459565 2313 20-21 -3.5262284301636484 2313 22-23 -3.5301883712211293 2313 24-25 -3.1096705736577164 2313 26-27 -2.9965927425309644 2313 28-29 -3.697967343174575 2313 30-31 -3.6499241520665464 2313 32-33 -3.440907524882185 2313 34-35 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-5.634560646498834 2314 1 -0.3617411216792661 2314 2 -0.8118015408769317 2314 3 -0.3264735031035464 2314 4 -0.3927975382667128 2314 5 -0.49464491990760706 2314 6 -0.6350882365659274 2314 7 -1.5849020446640978 2314 8 -0.9155684070713548 2314 9 -0.9190688405698779 2314 10-11 -1.681772958453216 2314 12-13 -2.5587188696227443 2314 14-15 -1.3288128161821362 2314 16-17 -1.4475778610864083 2314 18-19 -1.9781017980005018 2314 20-21 -2.6512284301636484 2314 22-23 -1.364279280312033 2314 24-25 -1.2914887554758963 2314 26-27 -1.4647745607127831 2314 28-29 -1.0411491613563868 2314 30-31 -1.8817423338847235 2314 32-33 -1.7749984339730887 2314 34-35 -0.6620724639423585 2314 36-37 -0.08833807671435068 2314 38-39 -0.1333600943009543 2314 40-41 -0.6654294422795175 2314 42-43 -1.0397303349630391 2314 44-45 -0.8965170944278142 2314 46-47 0.05457453101249854 2314 48-49 -0.3699848101956178 2314 50-51 -0.40452047649436196 2314 52-53 -1.4808359992578382 2314 54-55 -0.7668358813879443 2314 56-57 -1.329834796536062 2314 58-59 -1.8026919969922837 2314 60-61 -2.205990154457613 2314 62-63 -1.1903703842988875 2314 64-65 -0.8910777154421581 2314 66-67 0.0019540232573938 2314 68-69 -1.9554312778271346 2314 70-71 -1.4866497940633074 2314 72-73 -0.6159176150098311 2314 74-75 -1.7303077175546164 2314 76-77 -0.5059943613468789 2314 78-79 -0.321278590697581 2314 80-81 -0.85097636430012 2314 82-83 -1.8513964549189161 2314 84-85 -0.3518644839500773 2314 86-87 -1.6823229969750386 2314 88-89 -1.7421768910268405 2314 90-91 -0.6090392801800739 2314 92-93 -1.0176577857407096 2314 94-95 0.5061234955230312 2314 96-97 0.29555163669339635 2314 98-99 0.412091787790839 2314 100-101 2.20634844441026 2315 1 0.0273497874116444 2315 2 -0.37907426814965817 2315 3 -0.0228371394671818 2315 4 0.9653842799151064 2315 5 0.8689914437287598 2315 6 -0.3459973274750183 2315 7 0.8641888644268079 2315 8 0.2917043202013687 2315 9 0.8972947957937549 2315 10-11 -0.004500231180479375 2315 12-13 0.21582658492270923 2315 14-15 -0.8151764525457708 2315 16-17 0.06151304800449964 2315 18-19 -0.19264725254596016 2315 20-21 0.11877156983634762 2315 22-23 0.7948116287788736 2315 24-25 0.7703294263422862 2315 26-27 1.60340725746903 2315 28-29 1.5470326568254222 2315 30-31 1.5000758479334593 2315 32-33 1.0640924751178176 2315 34-35 1.007018445148546 2315 36-37 1.6898437414674703 2315 38-39 0.9284580875172281 2315 40-41 1.0563887395386686 2315 42-43 0.15481511958241612 2315 44-45 0.1753010873903733 2315 46-47 0.9154836219215952 2315 48-49 -0.8645302647410702 2315 50-51 -0.44179320376709086 2315 52-53 -0.8235632719851118 2315 54-55 0.7958913913393282 2315 56-57 0.49925611255484625 2315 58-59 0.3845807302804438 2315 60-61 -0.20053560900306522 2315 62-63 -0.05037038429888696 2315 64-65 0.6707404663760244 2315 66-67 0.5555903868937548 2315 68-69 0.8182050858092289 2315 70-71 1.1106229332094166 2315 72-73 0.4722642031719886 2315 74-75 1.3287831915362922 2315 76-77 0.8603692750167582 2315 78-79 0.4605395911206003 2315 80-81 0.8962963629726062 2315 82-83 1.4331489996265354 2315 84-85 2.399044606959013 2315 86-87 2.6013133666613264 2315 88-89 3.118732199882249 2315 90-91 2.898233447092654 2315 92-93 1.7932513051683827 2315 94-95 0.5943053137048508 2315 96-97 1.4837334548752175 2315 98-99 -0.25609003039098255 2315 100-101 0.7054393535011663 2316 1 -3.7629950401745624 2316 2 -0.6080397853910391 2316 3 -1.2904233463637347 2316 4 -0.9711674442228215 2316 5 -0.6137671769608986 2316 6 0.4291750863180823 2316 7 -0.03788010109042972 2316 8 0.15653190640826864 2316 9 0.5966051406213424 2316 10-11 -1.273465748421863 2316 12-13 -0.5352078978359103 2316 14-15 -0.801383349097506 2316 16-17 -1.6246938485472242 2316 18-19 -1.5016127697873358 2316 20-21 -1.6770904991291644 2316 22-23 -2.89484354363492 2316 24-25 -0.43725678055427153 2316 26-27 0.1034072574690299 2316 28-29 -1.1108983776573353 2316 30-31 -0.3206138072389564 2316 32-33 -1.0641833869511501 2316 34-35 -0.09780914105834881 2316 36-37 0.2815678793985015 2316 38-39 -0.10671432627587052 2316 40-41 0.49914736022831363 2316 42-43 0.06998753337551733 2316 44-45 8.183287696823527E-4 2316 46-47 1.6023801736457344 2316 48-49 1.3306421490520322 2316 50-51 0.6871723134742886 2316 52-53 -0.19321844439890512 2316 54-55 -0.8647982638330838 2316 56-57 0.027531974623812516 2316 58-59 1.148718661314927 2316 60-61 0.046360942721069165 2316 62-63 -0.6903703842988875 2316 64-65 -0.7609836715550138 2316 66-67 -1.0740647855200365 2316 68-69 -0.8590362935011164 2316 70-71 -2.3369632736871324 2316 72-73 -2.2373909692418046 2316 74-75 -2.0022512912223256 2316 76-77 -1.5506652077418615 2316 78-79 -2.5877362709483656 2316 80-81 -1.3478415680618774 2316 82-83 -1.3365061727872565 2316 84-85 -1.0540588413168521 2316 86-87 -1.1290314609248817 2316 88-89 -0.5391988346005085 2316 90-91 -0.29555965635562487 2316 92-93 -0.9267486948316161 2316 94-95 -2.684315375950323 2316 96-97 -2.9983355106420255 2316 98-99 -3.0354003752185683 2316 100-101 -3.427664094774695 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 185.0 3 55.0 4 40.0 5 34.0 6 48.0 7 55.0 8 75.0 9 68.0 10 108.0 11 151.0 12 197.0 13 301.0 14 306.0 15 415.0 16 547.0 17 717.0 18 707.0 19 377.0 20 400.0 21 378.0 22 385.0 23 402.0 24 391.0 25 386.0 26 411.0 27 394.0 28 471.0 29 534.0 30 538.0 31 690.0 32 861.0 33 1048.0 34 1493.0 35 2598.0 36 2716.0 37 4449.0 38 5184.0 39 1061.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.217040973769926 25.25287159266244 18.631921824104232 17.898165609463398 2 13.902698254877087 25.806562210717594 40.16525525422567 20.125484280179656 3 15.23639729831659 33.09562176432269 34.46360613021566 17.20437480714506 4 9.527075688466683 23.74909976336637 40.59810007201893 26.125724476148015 5 8.294440631434455 40.39121482498284 37.56005490734386 13.754289636238848 6 20.029510671882505 43.45618008372795 20.41040422757532 16.10390501681422 7 17.65149696491649 40.553516924448715 26.331492849549026 15.46349326108577 8 20.06449178415835 35.92672635587115 24.057493739494358 19.95128812047614 9 22.101797228700782 20.671559884757855 25.415008917546988 31.811633968994375 10-11 18.685370329086137 31.37550803422907 29.40510692298458 20.534014713700206 12-13 22.09627355821172 31.747637748015023 29.720645482139492 16.435443211633768 14-15 19.306548690776275 33.20472589467908 30.469674365965325 17.019051048579318 16-17 13.932514916672382 36.27837596872642 31.820519854605305 17.968589259995884 18-19 13.11570970562518 34.41116464073242 36.54053868705745 15.932586966584944 20-21 14.456219775697088 32.99893679047913 36.913948622972185 15.630894810851597 22-23 14.418955525837534 32.9235675342043 36.57374069883071 16.083736241127454 24-25 14.320970727359978 33.86577089189004 36.62336326866388 15.189895112086106 26-27 14.159383033419024 33.804627249357324 36.61868037703513 15.417309340188517 28-29 13.611434839240419 33.33961746760814 37.79906766298759 15.249880030163846 30-31 14.02618770137794 33.882909439912254 37.07582093645026 15.015081922259546 32-33 13.195420422993864 34.859287697528536 36.439515990813426 15.505775888664175 34-35 13.869989202899793 33.856621364547806 36.490771050060836 15.78261838249156 36-37 14.235034019434782 33.78121304563917 36.206276028723714 15.777476906202335 38-39 14.124622977789963 34.410131615026046 35.82225116534138 15.642994241842612 40-41 13.464274084357381 33.880062385383994 37.24613090647334 15.409532623785285 42-43 13.822238979913623 34.421059847809694 35.445945019537945 16.31075615273874 44-45 14.125107112253643 34.06169665809769 35.5235646958012 16.289631533847473 46-47 14.56898029134533 33.5681233933162 35.18423307626393 16.678663239074552 48-49 14.61817932547299 33.174527008500135 35.13332876336715 17.073964902659718 50-51 14.686980514472758 33.01057394046374 34.830594162910664 17.471851382152835 52-53 14.319011465492126 33.80863425251504 34.70153730141048 17.170816980582355 54-55 14.854743337046877 32.94198303196503 34.6387865284086 17.564487102579484 56-57 14.824335904027421 33.15338474721508 34.29991431019709 17.72236503856041 58-59 14.1608541413172 32.194820997069456 35.375571969632055 18.268752891981286 60-61 14.614751850836305 32.75637510282424 34.57465039758706 18.0542226487524 62-63 14.214468114277878 32.27304666746645 35.21739875576274 18.29508646249293 64-65 14.589045791061144 31.503633123114888 35.58404167809158 18.323279407732382 66-67 14.830813534917207 31.206074942576024 34.71665123932943 19.246460283177345 68-69 14.392497728402681 31.102881928371822 34.965454576625696 19.539165766599805 70-71 15.003515511121018 30.75472021676127 35.02820983314184 19.213554438975873 72-73 14.692625409175822 29.854838986100873 35.006598227904504 20.445937376818797 74-75 14.44486340109005 30.65162993178624 34.458231926781615 20.445274740342096 76-77 14.63096911316033 30.069246854753008 34.39203318364129 20.907750848445374 78-79 14.757105145874045 29.457643388528922 34.42867427748637 21.356577188110666 80-81 15.069550451949299 29.93327959110166 33.42881155343636 21.568358403512683 82-83 14.878115678677958 28.58024479720232 33.95618335790448 22.58545616621524 84-85 14.471541928739137 28.75456306020669 33.428165007112376 23.345730003941796 86-87 15.028624318672653 29.02025984710843 33.490452847005585 22.46066298721333 88-89 15.53837450499717 28.16244664255224 33.01733152760873 23.281847324841856 90-91 14.938705529361338 27.44277753964852 33.964852121731674 23.653664809258466 92-93 15.603298983213593 28.514600229762866 33.06184736201369 22.82025342500986 94-95 15.292202227934876 28.07712082262211 32.86203941730934 23.768637532133678 96-97 15.208747514910536 27.560499074518408 32.66435867553301 24.566394735038045 98-99 15.689770447960777 27.870257667449554 32.06699696559291 24.37297491899676 100-101 16.23786491029467 27.0899797605571 32.220163973791635 24.451991355356594 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 982.0 1 591.5 2 184.5 3 147.0 4 98.0 5 100.0 6 173.0 7 331.5 8 440.5 9 406.0 10 286.5 11 163.0 12 133.0 13 132.0 14 122.5 15 117.5 16 118.5 17 128.5 18 127.0 19 123.0 20 144.5 21 147.5 22 150.0 23 175.0 24 205.0 25 236.5 26 268.5 27 323.0 28 360.5 29 402.5 30 536.5 31 678.0 32 842.5 33 1063.5 34 1215.0 35 1286.5 36 1414.0 37 1491.5 38 1385.5 39 1286.5 40 1164.0 41 1028.5 42 900.5 43 838.5 44 802.0 45 723.5 46 641.0 47 627.0 48 952.5 49 932.5 50 598.0 51 441.0 52 254.0 53 157.5 54 140.5 55 138.0 56 122.0 57 109.5 58 114.0 59 93.5 60 71.5 61 73.0 62 62.5 63 43.0 64 30.0 65 24.0 66 17.0 67 11.5 68 10.0 69 7.5 70 5.5 71 5.0 72 3.0 73 1.0 74 1.5 75 1.5 76 1.0 77 0.5 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03770222100356457 2 0.030847271730189193 3 0.030847271730189193 4 0.0582670688236907 5 0.12338908692075677 6 0.1165341376473814 7 0.0582670688236907 8 0.08568686591719221 9 0.06854949273375377 10-11 0.06683575541540993 12-13 0.06683575541540993 14-15 0.05998080614203454 16-17 0.04798464491362764 18-19 0.042843432958596106 20-21 0.06512201809706608 22-23 0.04455717027693995 24-25 0.006854949273375377 26-27 0.0034274746366876885 28-29 0.006854949273375377 30-31 0.006854949273375377 32-33 0.010282423910063065 34-35 0.005141211955031533 36-37 0.005141211955031533 38-39 0.0 40-41 0.008568686591719221 42-43 0.006854949273375377 44-45 0.0034274746366876885 46-47 0.0034274746366876885 48-49 0.0 50-51 0.0017137373183438442 52-53 0.005141211955031533 54-55 0.01199616122840691 56-57 0.0034274746366876885 58-59 0.0017137373183438442 60-61 0.0 62-63 0.005141211955031533 64-65 0.0 66-67 0.02399232245681382 68-69 0.03941595832190842 70-71 0.06683575541540993 72-73 0.005141211955031533 74-75 0.010282423910063065 76-77 0.017137373183438442 78-79 0.02399232245681382 80-81 0.08397312859884837 82-83 0.030847271730189193 84-85 0.005141211955031533 86-87 0.017137373183438442 88-89 0.03256100904853304 90-91 0.046270907595283794 92-93 0.05312585686865917 94-95 0.0034274746366876885 96-97 0.006854949273375377 98-99 0.03598848368522073 100-101 0.08568686591719221 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 29176.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.12434877981903 #Duplication Level Percentage of deduplicated Percentage of total 1 75.73641719397774 35.690293391828895 2 10.55349479962179 9.946531395667673 3 4.873081678667539 6.8892240197422545 4 2.3638082769655977 4.455717027693995 5 1.6001163720997893 3.7702221003564573 6 1.076441923048949 3.0435974773786674 7 0.7927849298130772 2.6151631477927064 8 0.5818604989453778 2.1935837674801206 9 0.4654883991563023 1.9742253907321083 >10 1.8183140592043057 14.04236358650946 >50 0.0945523310786239 3.266383328763367 >100 0.02181976871045167 3.2081162599396764 >500 0.014546512473634447 5.161776802851659 >1k 0.0072732562368172235 3.742802303262956 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1092 3.742802303262956 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 963 3.3006580751302437 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 543 1.861118727721415 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 438 1.5012338908692076 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 310 1.0625171373731834 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 188 0.6443652316972854 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 99 0.33931998903208116 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 98 0.33589251439539347 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 85 0.2913353441184535 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 84 0.28790786948176583 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 75 0.25706059775157664 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 75 0.25706059775157664 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 75 0.25706059775157664 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 72 0.24677817384151354 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 70 0.23992322456813817 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 60 0.2056484782012613 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 56 0.19193857965451055 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 53 0.1816561557444475 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 51 0.1748012064710721 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47 0.16109130792432136 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 44 0.1508088840142583 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 41 0.14052646010419523 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 40 0.13709898546750754 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 39 0.13367151083081985 No Hit GTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 39 0.13367151083081985 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 38 0.13024403619413216 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 37 0.12681656155744447 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 35 0.11996161228406908 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 34 0.1165341376473814 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 34 0.1165341376473814 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 33 0.11310666301069372 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 32 0.10967918837400603 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32 0.10967918837400603 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 31 0.10625171373731834 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 30 0.10282423910063065 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 30 0.10282423910063065 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 30 0.10282423910063065 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 30 0.10282423910063065 No Hit TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 30 0.10282423910063065 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0034274746366876885 0.0 20-21 0.0 0.0 0.0 0.006854949273375377 0.0 22-23 0.0 0.0 0.0 0.013709898546750754 0.0 24-25 0.0 0.0 0.0 0.03256100904853304 0.0 26-27 0.0 0.0 0.0 0.06169454346037839 0.0 28-29 0.0 0.0 0.0 0.13024403619413216 0.0 30-31 0.0 0.0 0.0 0.20050726624622978 0.0 32-33 0.0 0.0 0.0 0.2519193857965451 0.0 34-35 0.0 0.0 0.0 0.2827666575267343 0.0 36-37 0.0 0.0 0.0 0.3221826158486427 0.0 38-39 0.0 0.0 0.0 0.37702221003564573 0.0 40-41 0.0 0.0 0.0 0.49698382231971483 0.0 42-43 0.0 0.0 0.0 0.7094872497943515 0.0 44-45 0.0 0.0 0.0 0.8585823964902659 0.0 46-47 0.0 0.0 0.0 1.278448039484508 0.0 48-49 0.0 0.0 0.0 1.672607622703592 0.0 50-51 0.0 0.0 0.0 1.939950644365232 0.0 52-53 0.0 0.0 0.0 2.265560734850562 0.0 54-55 0.0 0.0 0.0 2.52776254455717 0.0 56-57 0.0 0.0 0.0 3.0247463668768853 0.0 58-59 0.0 0.0 0.0 3.4394707979160954 0.0 60-61 0.0 0.0 0.0 3.7719358376748016 0.0 62-63 0.0 0.0 0.0 4.0941184535234445 0.0 64-65 0.0 0.0 0.0 4.529407732382781 0.0 66-67 0.0 0.0 0.0 5.098368522072937 0.0 68-69 0.0 0.0 0.0 5.753016177680285 0.0 70-71 0.0 0.0 0.0 6.251713737318344 0.0 72-73 0.0 0.0 0.0 6.64244584590074 0.0 74-75 0.0 0.0 0.0 7.14628461749383 0.0 76-77 0.0 0.0 0.0 7.734096517685769 0.0 78-79 0.0 0.0 0.0 8.291061146147518 0.0 80-81 0.0 0.0 0.0 9.079380312585688 0.0 82-83 0.0 0.0 0.0 9.82999725802029 0.0 84-85 0.0 0.0 0.0 10.671442281327117 0.0 86-87 0.0 0.0 0.0 11.439196599945161 0.0 88-89 0.0 0.0 0.0 12.380038387715931 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCTAGC 15 6.4981607E-4 95.19965 8 TATAAGC 20 0.0020366912 71.39974 2 GCAGAAA 45 9.373366E-4 42.31096 9 CGCAGAA 50 0.0015737023 38.07986 8 CAGAAAA 55 2.6315993E-6 30.2908 10-11 GTACATG 130 2.8162322E-7 29.2922 1 GAAGTAT 35 0.008382022 27.10659 54-55 AGTACAT 90 8.6320227E-4 26.44435 2 GGTATCA 350 0.0 25.839909 1 ACATGGG 130 9.434931E-6 25.630676 3 AGTACTT 345 0.0 24.834692 12-13 GTATCAA 465 0.0 24.567654 1 TACATGG 140 1.6758675E-5 23.799915 2 AAACCTC 55 0.002838763 21.59911 74-75 AACGCAG 555 0.0 21.441362 6 CAACGCA 555 0.0 21.441362 5 TCAACGC 545 0.0 20.961393 4 TATCAAC 560 0.0 20.399927 2 ATCAACG 560 0.0 20.399927 3 TACTTTT 410 0.0 20.317 14-15 >>END_MODULE