FastQCFastQC Report
Thu 26 May 2016
SRR936975_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936975_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631213
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91081.4429360612027953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64201.017089318502629No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45540.7214680306013976No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23220.3678631460378668No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA17470.2767686977296095No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT17110.27106539313987515No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14200.22496368103952233No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14140.2240131302745666No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10940.17331708947692775No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT10520.16666323412223766No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG9760.15462292443279843No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA9430.14939489522554195No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9310.14749379369563048No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC7410.11739301947203241No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG6960.11026388873486447No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6400.10139208159527767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88300.032.8901561
CTACACG1102.7166006E-630.2475934
GATCGGT650.005829718429.243466
GGTATCA57700.028.4203051
ATCAACG103750.027.8090423
TATCAAC104000.027.7421952
TCAACGC104450.027.531664
CAACGCA105350.027.3372385
GCGTAAG700.00836129127.1611042
AACGCAG106850.026.9958066
GAGTACT56850.025.9127412-13
GTACATG53500.025.4984381
CGCAGTA950.001221146625.0168062
ACGCAGA116000.024.8254287
CGCAGAG116600.024.6996428
TACATGG54250.024.6202262
GTACTTT61000.024.0719214-15
GCAGAGT116050.024.038629
AGTACTT57250.023.40753712-13
ACATGGG54600.023.1565823
Produced by FastQC (version 0.11.5)