##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936975_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 631213 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.811458255771033 33.0 31.0 34.0 30.0 34.0 2 31.853968470231127 33.0 31.0 34.0 30.0 34.0 3 31.827124916628776 33.0 31.0 34.0 30.0 34.0 4 35.43055355323797 37.0 35.0 37.0 33.0 37.0 5 35.47065570576018 37.0 35.0 37.0 33.0 37.0 6 35.5641027672117 37.0 35.0 37.0 33.0 37.0 7 35.51514148156011 37.0 35.0 37.0 33.0 37.0 8 35.479565534930366 37.0 35.0 37.0 33.0 37.0 9 37.071764998502886 39.0 37.0 39.0 33.0 39.0 10-11 36.95399492722741 39.0 37.0 39.0 33.0 39.0 12-13 36.84277177434558 39.0 37.0 39.0 33.0 39.0 14-15 37.876782480715704 40.0 37.5 41.0 33.0 41.0 16-17 37.84814951529832 40.0 37.5 41.0 33.0 41.0 18-19 37.92397178131629 40.0 38.0 41.0 33.0 41.0 20-21 37.933431345678876 40.0 38.0 41.0 33.0 41.0 22-23 37.80850758777149 40.0 37.5 41.0 33.0 41.0 24-25 37.629579872404406 40.0 37.0 41.0 32.0 41.0 26-27 37.5267302796362 40.0 37.0 41.0 32.0 41.0 28-29 37.54919020996082 40.0 37.0 41.0 32.0 41.0 30-31 37.28823392420625 39.0 36.5 41.0 32.0 41.0 32-33 36.967476113451404 39.0 35.0 41.0 31.5 41.0 34-35 36.71020875679049 39.0 35.0 41.0 31.0 41.0 36-37 36.4964900913004 39.0 35.0 41.0 30.0 41.0 38-39 36.254353918566316 39.0 35.0 41.0 30.0 41.0 40-41 35.95412325158068 39.0 35.0 40.0 30.0 41.0 42-43 35.655701007425385 38.0 35.0 40.0 28.5 41.0 44-45 35.20901660770612 38.0 35.0 40.0 26.5 41.0 46-47 34.57568047552886 38.0 34.5 40.0 23.5 41.0 48-49 34.00130383879926 38.0 34.0 40.0 19.5 41.0 50-51 33.25923103611618 37.5 33.0 39.5 15.5 40.5 52-53 33.48094620991647 38.0 33.5 40.0 15.0 40.5 54-55 33.75904567871701 38.0 34.0 40.0 13.5 41.0 56-57 33.639367376780896 38.0 34.0 40.0 10.5 41.0 58-59 33.40315868019195 38.0 33.5 40.0 8.0 41.0 60-61 33.10516022325269 37.5 33.0 40.0 7.0 41.0 62-63 32.794338044368544 37.0 33.0 40.0 4.0 41.0 64-65 32.457865253092066 36.5 32.5 40.0 2.0 41.0 66-67 32.076791035672585 36.0 32.0 39.0 2.0 41.0 68-69 31.65069081276843 35.5 31.5 39.0 2.0 41.0 70-71 31.2813566894218 35.0 31.0 38.5 2.0 40.0 72-73 30.899198844130268 35.0 31.0 37.5 2.0 40.0 74-75 30.464890615370724 35.0 30.5 37.0 2.0 39.5 76-77 30.02432697045213 35.0 30.0 36.5 2.0 39.0 78-79 29.60693379255497 35.0 30.0 36.0 2.0 39.0 80-81 29.199609323635602 34.0 29.0 36.0 2.0 37.5 82-83 28.799144662736666 34.0 29.0 35.0 2.0 37.0 84-85 28.456263575053114 34.0 29.0 35.0 2.0 37.0 86-87 28.120498944096525 34.0 28.5 35.0 2.0 36.0 88-89 27.738505068811953 34.0 27.0 35.0 2.0 36.0 90-91 27.36996703173097 34.0 26.5 35.0 2.0 35.5 92-93 27.027025742498964 34.0 26.0 35.0 2.0 35.0 94-95 26.653857731067006 34.0 25.0 35.0 2.0 35.0 96-97 26.209720015272183 33.5 24.5 35.0 2.0 35.0 98-99 25.654092200255697 33.0 21.5 35.0 2.0 35.0 100-101 23.691255566662917 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4242971880616011 1101 2 0.2789753715293841 1101 3 0.3556842157728006 1101 4 0.15480845298717583 1101 5 0.22381283381993455 1101 6 0.1956791563570519 1101 7 0.24825899901919257 1101 8 0.204726013425919 1101 9 0.3516061247559108 1101 10-11 0.3634231065060689 1101 12-13 0.45910221332666623 1101 14-15 0.594666008374773 1101 16-17 0.6083877211267534 1101 18-19 0.509450504816094 1101 20-21 0.4017237756694456 1101 22-23 0.5528118656486569 1101 24-25 0.41006924335987804 1101 26-27 0.6026312919326458 1101 28-29 0.6247067201712753 1101 30-31 0.6362251565332784 1101 32-33 0.5557842767150873 1101 34-35 0.4408824139542915 1101 36-37 0.40012165941801214 1101 38-39 0.5587818947471419 1101 40-41 0.6537779979365155 1101 42-43 0.4989040917678196 1101 44-45 0.5915491455992381 1101 46-47 0.8282917013592765 1101 48-49 0.8175139695224303 1101 50-51 0.8154483413052205 1101 52-53 0.7475764853483895 1101 54-55 0.97119709991577 1101 56-57 1.0800644744105554 1101 58-59 0.9823488285514017 1101 60-61 0.9761388691782713 1101 62-63 0.961816633127448 1101 64-65 0.954018753992564 1101 66-67 0.9344796802149169 1101 68-69 0.8275763259071987 1101 70-71 0.8777568251525594 1101 72-73 1.035002048852224 1101 74-75 0.9267400332090645 1101 76-77 0.8221640293577615 1101 78-79 0.8765484937389942 1101 80-81 1.132572417073714 1101 82-83 1.0141487652090184 1101 84-85 1.1620967513285656 1101 86-87 1.1853657828048298 1101 88-89 1.1661999525068083 1101 90-91 1.2042113213861114 1101 92-93 1.199918935089027 1101 94-95 1.187302041677949 1101 96-97 1.1777325993927867 1101 98-99 1.409698250495726 1101 100-101 1.6258156280532567 1102 1 0.34550900913971105 1102 2 0.31212817425580397 1102 3 0.22584174110786037 1102 4 0.24341326503575544 1102 5 0.19865785212060416 1102 6 0.2072455707610814 1102 7 0.17770649732718624 1102 8 0.16720701119142234 1102 9 0.25322219633186904 1102 10-11 0.4307461662522556 1102 12-13 0.3181469017800609 1102 14-15 0.47183428901207947 1102 16-17 0.2530999601414692 1102 18-19 0.410050997936267 1102 20-21 0.43163363041959 1102 22-23 0.38478934359256556 1102 24-25 0.3998673131980581 1102 26-27 0.3738785288239157 1102 28-29 0.5720193202472075 1102 30-31 0.47870920065897593 1102 32-33 0.6932092619710062 1102 34-35 0.46663653817075357 1102 36-37 0.5599268874523275 1102 38-39 0.4971084825686063 1102 40-41 0.6917640837238181 1102 42-43 0.7241970600781684 1102 44-45 0.9112684002464349 1102 46-47 0.8007450267690714 1102 48-49 1.2394382337063874 1102 50-51 1.160195395865081 1102 52-53 1.2748256521013417 1102 54-55 1.3001773646039538 1102 56-57 1.3109740755474562 1102 58-59 1.0265049192459017 1102 60-61 1.2289567523631106 1102 62-63 1.2071386694166577 1102 64-65 1.3910033676225453 1102 66-67 1.1340196498113464 1102 68-69 1.1080005353166946 1102 70-71 1.1559285288182473 1102 72-73 1.2420543727148292 1102 74-75 1.2851863294803465 1102 76-77 1.1944798933910512 1102 78-79 1.2279690280075428 1102 80-81 1.3234062493798397 1102 82-83 1.2535105152304062 1102 84-85 1.3596014371707952 1102 86-87 1.4838231690277794 1102 88-89 1.275783006340344 1102 90-91 1.1967732502873645 1102 92-93 1.1092923453166925 1102 94-95 1.3096494197822715 1102 96-97 1.2385029833692336 1102 98-99 1.36811618410308 1102 100-101 1.517969224634129 1103 1 0.30902666351632746 1103 2 0.31303446252165656 1103 3 0.178256515643195 1103 4 0.0024517508894277285 1103 5 0.10693435150580655 1103 6 0.021165411770290632 1103 7 0.04640331759613758 1103 8 0.024893447331621132 1103 9 0.15959616922638276 1103 10-11 0.2047036165273184 1103 12-13 0.01153719210628168 1103 14-15 0.204688002375498 1103 16-17 0.4641344440705524 1103 18-19 0.39612275594033264 1103 20-21 0.18276118709867006 1103 22-23 0.2612116773187907 1103 24-25 0.41794640636669556 1103 26-27 0.40629615460323265 1103 28-29 0.18326408341999922 1103 30-31 0.37406939508665715 1103 32-33 0.47936469040860175 1103 34-35 0.5320556158781855 1103 36-37 0.5097976056009159 1103 38-39 0.640698317544377 1103 40-41 0.6955670152439026 1103 42-43 0.7865451104128098 1103 44-45 0.7159441858023357 1103 46-47 0.7934336225568615 1103 48-49 0.8286270123641515 1103 50-51 0.6888593080264016 1103 52-53 0.5949253098672074 1103 54-55 0.6496350766673444 1103 56-57 0.7343261031977377 1103 58-59 0.7915887212100259 1103 60-61 0.6945449529650958 1103 62-63 0.7249810075046028 1103 64-65 0.8214084054991204 1103 66-67 0.8948185242488442 1103 68-69 0.90984586721083 1103 70-71 0.6574466465220077 1103 72-73 0.7972509770190186 1103 74-75 0.9813116077099977 1103 76-77 0.9347689043063951 1103 78-79 1.2290110897963729 1103 80-81 1.207304188422615 1103 82-83 1.0679047149855023 1103 84-85 1.0442483369096855 1103 86-87 1.3383817226324375 1103 88-89 1.3127108588359881 1103 90-91 0.9880121242835926 1103 92-93 0.957514518321009 1103 94-95 0.8136594059248878 1103 96-97 0.6770230349971165 1103 98-99 0.8426539813304146 1103 100-101 1.077013735437884 1104 1 0.3771945645743102 1104 2 0.4362739463568701 1104 3 0.3758623030537471 1104 4 0.36218370098932695 1104 5 0.38689944288950073 1104 6 0.3513434859410438 1104 7 0.28040837733411905 1104 8 0.44989244164165143 1104 9 0.4883551031056186 1104 10-11 0.5213411926804028 1104 12-13 0.5003338058364051 1104 14-15 0.7558960595165658 1104 16-17 0.5783093089728695 1104 18-19 0.5781559928764111 1104 20-21 0.5781521099644849 1104 22-23 0.8662599057400371 1104 24-25 0.6611501770336261 1104 26-27 0.778227682760317 1104 28-29 0.8321017293009092 1104 30-31 0.9249628947536692 1104 32-33 1.0910163909185968 1104 34-35 1.0268729602308966 1104 36-37 1.091661327905335 1104 38-39 0.9250247510995564 1104 40-41 1.2378339298317655 1104 42-43 1.2657563812920571 1104 44-45 1.097978428428398 1104 46-47 1.139362477813492 1104 48-49 1.3190573135794708 1104 50-51 1.0877220961565754 1104 52-53 1.4215119534104588 1104 54-55 1.4607424754167155 1104 56-57 1.4282683855329097 1104 58-59 1.5550638493796285 1104 60-61 1.7012596096672397 1104 62-63 1.6782622386479247 1104 64-65 1.8372374069160315 1104 66-67 1.5161069695415428 1104 68-69 1.5161864072439961 1104 70-71 1.6192002569490036 1104 72-73 1.5753957695563514 1104 74-75 1.5064248037033252 1104 76-77 1.5362491681994435 1104 78-79 1.5997128826063616 1104 80-81 1.542368798416046 1104 82-83 1.55827635095255 1104 84-85 1.6725335908128791 1104 86-87 1.7630438108023938 1104 88-89 1.6331415623272996 1104 90-91 1.4240815303007501 1104 92-93 1.3492033846625766 1104 94-95 1.1942510421601185 1104 96-97 1.126452473553929 1104 98-99 1.144123517863779 1104 100-101 1.2676461803372483 1105 1 0.28244802079551334 1105 2 0.09857809382230798 1105 3 0.16217566710904308 1105 4 0.16342794062066446 1105 5 0.13247547975586116 1105 6 0.07664302511155086 1105 7 0.1645097598226002 1105 8 0.18403990707943763 1105 9 0.20618920448811195 1105 10-11 0.21459004060666587 1105 12-13 0.288260073117506 1105 14-15 0.279513571037306 1105 16-17 0.4205212905396891 1105 18-19 0.3314739652266425 1105 20-21 0.1742120177985882 1105 22-23 0.2523658965234503 1105 24-25 0.3555741401672705 1105 26-27 0.3737345952027127 1105 28-29 0.31790818091381823 1105 30-31 0.2753315583481353 1105 32-33 0.2865002618863386 1105 34-35 0.0646609786640795 1105 36-37 0.21647699610810633 1105 38-39 0.16281276953273505 1105 40-41 0.3384330081727782 1105 42-43 0.2958946301399763 1105 44-45 0.3250521611472905 1105 46-47 0.4860214639886067 1105 48-49 0.3799789725656453 1105 50-51 0.20097094408284022 1105 52-53 0.18303269073764739 1105 54-55 0.35237842011717646 1105 56-57 0.4727153901411967 1105 58-59 0.20815140698331192 1105 60-61 0.32066053132622585 1105 62-63 0.3132391971732673 1105 64-65 0.36956459585611867 1105 66-67 0.3644940663157321 1105 68-69 0.42037995563109476 1105 70-71 0.3166656947370221 1105 72-73 0.446916506883543 1105 74-75 0.31508839817337275 1105 76-77 0.08535055068792374 1105 78-79 0.4229386890579754 1105 80-81 0.6230139597063697 1105 82-83 0.8387141389709853 1105 84-85 0.7294690746838484 1105 86-87 0.6515921978991699 1105 88-89 0.5711139125577134 1105 90-91 0.6344962768445299 1105 92-93 0.3806089146164986 1105 94-95 0.605149659671639 1105 96-97 0.7678349620331879 1105 98-99 0.5342387252370493 1105 100-101 0.884443415360284 1106 1 0.44963861038922204 1106 2 0.44272762113702413 1106 3 0.46581426132135917 1106 4 0.3046908402381874 1106 5 0.4221601156799437 1106 6 0.38656292119295443 1106 7 0.3190453735918126 1106 8 0.10806675271702204 1106 9 0.38617621466347174 1106 10-11 0.428614721273469 1106 12-13 0.3831882682535408 1106 14-15 0.7447297072861332 1106 16-17 0.7681463176018255 1106 18-19 0.6286797096157599 1106 20-21 0.5975633925550312 1106 22-23 0.6135292074854135 1106 24-25 0.551775338117757 1106 26-27 0.5878069052265289 1106 28-29 0.5345986622589862 1106 30-31 0.27562888100094796 1106 32-33 0.310544427290111 1106 34-35 0.5377287422492714 1106 36-37 0.195681730177526 1106 38-39 0.29458010734034445 1106 40-41 0.3054763797539337 1106 42-43 0.5130166623258745 1106 44-45 0.47494752089327363 1106 46-47 0.31036295204518893 1106 48-49 0.18941680182162202 1106 50-51 0.08949659898649998 1106 52-53 0.25548704593717986 1106 54-55 0.29359513369154655 1106 56-57 0.27892019637631194 1106 58-59 0.3549999311949037 1106 60-61 0.4099035132126332 1106 62-63 0.20272898572778786 1106 64-65 0.4099456307121443 1106 66-67 0.22971741913380228 1106 68-69 0.33413040666667015 1106 70-71 0.47314978220184045 1106 72-73 0.38211027176771495 1106 74-75 0.498888643536727 1106 76-77 0.5956789045884783 1106 78-79 0.5626557995436485 1106 80-81 0.6374831810385508 1106 82-83 0.6417681910746147 1106 84-85 0.9174910130543452 1106 86-87 1.1145899607627072 1106 88-89 1.0348621986225162 1106 90-91 1.0268712497823955 1106 92-93 0.9697883906935694 1106 94-95 1.044578107387597 1106 96-97 1.0619982008294677 1106 98-99 1.2095378520321738 1106 100-101 1.4224299925414954 1107 1 0.1835882115035652 1107 2 0.1002065512511301 1107 3 0.3148990051762688 1107 4 0.2254202311461242 1107 5 0.20866131866841897 1107 6 0.18700753447788543 1107 7 0.15391937637767938 1107 8 0.26923906850501567 1107 9 0.23420570546869612 1107 10-11 0.4435641394772958 1107 12-13 0.5657822209500694 1107 14-15 0.18209692127738464 1107 16-17 0.45701550843377703 1107 18-19 0.35474305293882935 1107 20-21 0.5847807606132207 1107 22-23 0.666495875559086 1107 24-25 0.33502636171321853 1107 26-27 0.23580262793417006 1107 28-29 -0.008941078570870786 1107 30-31 0.43272792001717164 1107 32-33 0.35743283779761015 1107 34-35 0.26911759596305984 1107 36-37 0.3328927667174213 1107 38-39 0.24646056827486973 1107 40-41 0.2975268067796648 1107 42-43 0.4869448769784199 1107 44-45 0.7908058637238824 1107 46-47 0.5887738816527701 1107 48-49 0.7331324181597481 1107 50-51 0.4016404510822298 1107 52-53 0.5493679852530988 1107 54-55 0.5547210183453544 1107 56-57 0.53823104578467 1107 58-59 0.41654932407265477 1107 60-61 0.18559188995204323 1107 62-63 0.27571028664375063 1107 64-65 0.3558674294261479 1107 66-67 0.04897367678438869 1107 68-69 0.2639209037612069 1107 70-71 0.24091672604830805 1107 72-73 0.10690027641082978 1107 74-75 0.19956259848445512 1107 76-77 -0.17302369696204067 1107 78-79 -0.10634649387297657 1107 80-81 -0.14039308702386677 1107 82-83 0.26853222242266384 1107 84-85 -0.059553715604916846 1107 86-87 3.5530062453403843E-4 1107 88-89 0.20207759687501792 1107 90-91 -0.006861672705753108 1107 92-93 0.2190250909876319 1107 94-95 0.1304123904486758 1107 96-97 0.2737874879005062 1107 98-99 0.21787435112465303 1107 100-101 0.4393281113770584 1108 1 0.1090206014406725 1108 2 0.15289260995134057 1108 3 -0.18165497805224717 1108 4 0.1442881556744311 1108 5 0.02336257653005447 1108 6 0.2240489390900393 1108 7 0.2789586845537855 1108 8 0.23112324041277077 1108 9 0.17817845179993697 1108 10-11 0.17985213738085548 1108 12-13 0.014557116128266046 1108 14-15 0.29366007641365144 1108 16-17 0.409708118496674 1108 18-19 0.4001702017857909 1108 20-21 0.39848621134697737 1108 22-23 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Count 2 1592.0 3 475.0 4 437.0 5 434.0 6 554.0 7 657.0 8 882.0 9 1019.0 10 1434.0 11 2047.0 12 2972.0 13 3799.0 14 4712.0 15 6192.0 16 8511.0 17 12032.0 18 9907.0 19 5523.0 20 4665.0 21 4443.0 22 4694.0 23 5146.0 24 5658.0 25 6229.0 26 7200.0 27 8539.0 28 9804.0 29 11597.0 30 14117.0 31 17249.0 32 21740.0 33 28514.0 34 39306.0 35 58132.0 36 81721.0 37 113953.0 38 104498.0 39 20810.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.106166913436205 18.77562149463078 15.100234933055177 20.01797665887784 2 14.912019181975033 21.248763067086166 41.433446665990054 22.405771084948746 3 18.774435351155617 28.43949862543718 30.94652998195787 21.839536041449332 4 11.725297649737053 17.29279532961585 38.628709219408115 32.353197801238984 5 12.269452141047948 38.706029572932344 33.25630561974532 15.768212666274392 6 27.976743669691594 37.485766123532606 18.55970615569561 15.977784051080187 7 25.736101154034042 36.35016907645554 20.847634515705394 17.066095253805013 8 26.469706700920575 32.493478278106835 20.29147537603971 20.74533964493288 9 28.28247259484144 16.230230296916314 20.815865568398294 34.67143153984395 10-11 23.953300999514678 27.54105432779387 26.753670861181337 21.75197381151012 12-13 26.977757585992823 25.907777986567176 27.47988287707564 19.634581550364363 14-15 23.692609246905818 25.46296773518285 28.737104103612793 22.10731891429854 16-17 19.87593795946334 29.41802499956383 28.743550661309996 21.962486379662835 18-19 19.637654099284774 26.601050836411556 34.008599193994044 19.75269587030963 20-21 21.62712127745929 25.125802217036174 33.69810955811476 19.548966947389783 22-23 20.770927170481983 25.556126307378396 33.4998026149946 20.17314390714502 24-25 20.28106274373038 25.62093782695539 34.16759138898576 19.930408040328462 26-27 20.311962526452614 25.665971831432444 33.99361174297966 20.028453899135286 28-29 19.663971857088164 25.714491808164645 34.48830456870496 20.13323176604223 30-31 19.89919282937673 26.060872631883434 34.23457479899986 19.805359739739977 32-33 19.44282191969731 26.246383002668516 33.82109465338248 20.489700424251694 34-35 19.997606690488816 25.978740754653284 33.69232976001128 20.331322794846617 36-37 20.364457146208455 25.789864531248636 33.54842851334676 20.297249809196153 38-39 20.350215367789538 25.862838258195666 33.618852507736996 20.168093866277804 40-41 20.04018642697484 26.068626052218573 33.64935559043135 20.24183193037523 42-43 19.75798589479591 26.612991435107027 32.934573602121645 20.69444906797542 44-45 19.904217565449215 27.0246580727173 32.30949312816904 20.761631233664442 46-47 20.19535611246566 26.984563428137655 31.331542001088746 21.488538458307943 48-49 20.556960861695686 27.261301355750607 30.691727607291952 21.49001017526175 50-51 20.992289901601367 27.6900367717261 30.2144581578007 21.103215168871834 52-53 20.67304415906328 28.015956409902863 30.075883284281797 21.23511614675206 54-55 20.141949298614186 27.84851556314929 30.36504078506394 21.64449435317258 56-57 20.65875002179402 28.509035404584193 29.27174686601987 21.560467707601912 58-59 20.69294563714985 27.773779784171747 29.743275973573084 21.789998605105314 60-61 20.715193725708996 27.532683882502866 30.256763439701253 21.49535895208689 62-63 21.430218817392213 27.38399523831036 30.054520078273043 21.131265866024375 64-65 21.08417838727494 27.59774349400254 30.35403117870662 20.964046940015894 66-67 20.746258482912115 27.914943399695957 29.984924551422182 21.35387356596975 68-69 20.750565872648536 28.23696590815427 29.891231985594818 21.121236233602374 70-71 20.64814088531082 28.235613837844348 29.55742382141977 21.55882145542506 72-73 20.625871014921046 28.192083994558608 29.1297496309947 22.052295359525644 74-75 20.56910221241813 28.35717534376392 29.478280712930783 21.59544173088717 76-77 20.784460412042215 28.030900392357005 29.569817911798523 21.614821283802257 78-79 20.49003933852933 28.050519454063966 29.59092843843556 21.86851276897115 80-81 20.34206416060517 28.714735479944558 29.308192946184686 21.635007413265587 82-83 20.24376730423672 28.674482586973976 29.02419363148604 22.05755647730326 84-85 20.370326336113834 28.050622838138175 28.796237290392533 22.782813535355455 86-87 20.504160249464363 28.10778204842646 29.030987259657117 22.357070442452063 88-89 20.778519594994755 27.568961648816277 29.07294518348678 22.579573572702184 90-91 20.225890268281354 27.731284272336566 29.671936752627246 22.37088870675483 92-93 20.13309921400911 28.065384295244023 29.291815468522707 22.50970102222416 94-95 19.864848473797743 28.201861583844607 29.147795423969736 22.785494518387917 96-97 19.811244457694528 28.084503111302816 28.84012787740008 23.264124553602578 98-99 19.88748440478013 28.110577148055643 28.819728379453412 23.182210067710816 100-101 20.174786711514685 28.377500592853554 28.784606587138367 22.66310610849339 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1045.0 1 746.5 2 512.5 3 600.0 4 533.0 5 570.5 6 1016.5 7 2154.0 8 3521.5 9 3895.5 10 3084.5 11 2325.0 12 2250.0 13 2326.0 14 2375.5 15 2312.0 16 2258.5 17 2331.5 18 2366.0 19 2431.5 20 2626.5 21 2863.0 22 3174.5 23 3624.0 24 4046.5 25 4605.5 26 5427.5 27 6314.5 28 7017.5 29 7819.5 30 8775.5 31 9700.0 32 11048.0 33 12238.0 34 13389.5 35 14246.0 36 15366.5 37 16978.5 38 17406.0 39 17169.5 40 17121.5 41 17791.5 42 18228.0 43 18580.5 44 19422.5 45 19996.0 46 20240.0 47 21227.5 48 28545.0 49 28747.0 50 21360.0 51 19100.0 52 17601.0 53 17092.5 54 16601.5 55 15362.5 56 13946.0 57 12560.5 58 11533.0 59 10719.5 60 9748.5 61 8528.0 62 7049.0 63 5130.5 64 3471.5 65 2584.0 66 1878.0 67 1441.5 68 1042.0 69 645.0 70 465.5 71 352.5 72 275.0 73 256.0 74 187.5 75 121.5 76 90.0 77 55.5 78 30.0 79 21.0 80 20.5 81 17.0 82 12.0 83 11.0 84 9.5 85 4.0 86 0.5 87 2.5 88 2.5 89 0.5 90 0.5 91 2.0 92 2.5 93 2.0 94 2.5 95 1.5 96 2.0 97 2.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06257792535958542 2 0.09838200417291786 3 0.07398453453905417 4 0.1359287593886691 5 0.11153128975480543 6 0.1069369610575194 7 0.11549191794212096 8 0.0994909800653662 9 0.12135364765934796 10-11 0.1120065651372833 12-13 0.09402531316687077 14-15 0.10052074339406823 16-17 0.1143829420496726 18-19 0.09069838548952572 20-21 0.07303398377409845 22-23 0.07382610941156155 24-25 0.06226107510460019 26-27 0.05830044691728466 28-29 0.06851886764055873 30-31 0.04839887644899583 32-33 0.0534684805287597 34-35 0.04554722415412864 36-37 0.05220107950881874 38-39 0.049983127723922036 40-41 0.0638453263795264 42-43 0.060518398702181356 44-45 0.048161238757756894 46-47 0.03350691446468941 48-49 0.04293320955050039 50-51 0.024951957580087863 52-53 0.03865573110819961 54-55 0.044913523644158154 56-57 0.048478089012742136 58-59 0.054022968474983876 60-61 0.05085446592513145 62-63 0.05457745642120806 64-65 0.03762596777949757 66-67 0.061152099212151846 68-69 0.052121866945072426 70-71 0.02320928117766903 72-73 0.019723928372831358 74-75 0.02915022345864233 76-77 0.04475509851666553 78-79 0.06439981432575058 80-81 0.03952706930940903 82-83 0.06439981432575058 84-85 0.06194422484961494 86-87 0.0667761912381399 88-89 0.08784673319465854 90-91 0.06653855354690097 92-93 0.07612327376020456 94-95 0.08135130296746106 96-97 0.0964809026430064 98-99 0.12856199096026222 100-101 0.12475978790043932 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 631213.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.49659439715581 #Duplication Level Percentage of deduplicated Percentage of total 1 81.36811151839687 49.22493639390998 2 11.251248207925455 13.61324398593185 3 3.24353354293866 5.886681994821895 4 1.3701253484153089 3.315516699053709 5 0.7073778622394944 2.1396975808714926 6 0.4458475416981874 1.6183354735850555 7 0.27928325220981787 1.1826979941599145 8 0.21427444523372152 1.0370299362384023 9 0.16138600054931307 0.8786973074949862 >10 0.8668773030970969 9.623931903675123 >50 0.06024231408710522 2.5170500928980832 >100 0.026716129316661036 3.0380461343757976 >500 0.002357305527933074 1.0370840217730246 >1k 0.0020953826914960655 2.4265706202340867 >5k 5.238456728740164E-4 2.460479860976551 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9108 1.4429360612027953 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6420 1.017089318502629 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4554 0.7214680306013976 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2322 0.3678631460378668 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1747 0.2767686977296095 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1711 0.27106539313987515 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1420 0.22496368103952233 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1414 0.2240131302745666 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1094 0.17331708947692775 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1052 0.16666323412223766 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 976 0.15462292443279843 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 943 0.14939489522554195 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 931 0.14749379369563048 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 741 0.11739301947203241 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 696 0.11026388873486447 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 640 0.10139208159527767 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.5842512749262135E-4 0.0 0.0 0.0 0.0 6 1.5842512749262135E-4 0.0 0.0 0.0 0.0 7 1.5842512749262135E-4 0.0 0.0 0.0 0.0 8 1.5842512749262135E-4 0.0 0.0 0.0 0.0 9 1.5842512749262135E-4 0.0 0.0 1.5842512749262135E-4 0.0 10-11 1.5842512749262135E-4 0.0 0.0 1.5842512749262135E-4 0.0 12-13 1.5842512749262135E-4 0.0 0.0 1.5842512749262135E-4 0.0 14-15 1.5842512749262135E-4 0.0 0.0 1.5842512749262135E-4 0.0 16-17 1.5842512749262135E-4 0.0 0.0 1.5842512749262135E-4 0.0 18-19 2.3763769123893202E-4 0.0 0.0 1.5842512749262135E-4 0.0 20-21 3.168502549852427E-4 0.0 0.0 4.7527538247786404E-4 0.0 22-23 3.168502549852427E-4 0.0 0.0 8.713382012094174E-4 0.0 24-25 4.7527538247786404E-4 0.0 0.0 0.0016634638386725242 0.0 26-27 4.7527538247786404E-4 0.0 0.0 0.002138739221150388 0.0 28-29 5.544879462241747E-4 0.0 0.0 0.0038022030598229123 0.0 30-31 6.337005099704854E-4 0.0 0.0 0.006653855354690097 0.0 32-33 6.337005099704854E-4 0.0 0.0 0.010059995595781456 0.0 34-35 6.337005099704854E-4 0.0 0.0 0.012198734816931844 0.0 36-37 6.337005099704854E-4 0.0 0.0 0.01639700069548631 0.0 38-39 7.921256374631068E-4 0.0 0.0 0.02146660477525019 0.0 40-41 7.921256374631068E-4 0.0 0.0 0.0259817209087899 0.0 42-43 7.921256374631068E-4 0.0 0.0 0.038022030598229124 0.0 44-45 7.921256374631068E-4 0.0 0.0 0.051171316180116694 0.0 46-47 0.0011881884561946601 0.0 0.0 0.06257792535958542 0.0 48-49 0.0012674010199409708 0.0 0.0 0.08103445271247582 0.0 50-51 0.0012674010199409708 0.0 0.0 0.1039268836351596 0.0 52-53 0.0012674010199409708 0.0 0.0 0.15137520931919968 0.0 54-55 0.0012674010199409708 0.0 0.0 0.18377314789144075 0.0 56-57 0.0012674010199409708 0.0 0.0 0.24674713606975773 0.0 58-59 0.001425826147433592 0.0 0.0 0.322791197266216 0.0 60-61 0.001425826147433592 0.0 0.0 0.40414250023367704 0.0 62-63 0.001425826147433592 0.0 0.0 0.5253377227655325 0.0 64-65 0.001425826147433592 0.0 0.0 0.5900543873462682 0.0 66-67 0.0017426764024188349 0.0 0.0 0.7270921226273857 0.0 68-69 0.0017426764024188349 0.0 0.0 0.9042114151641364 0.0 70-71 0.0017426764024188349 0.0 0.0 1.017881444140092 0.0 72-73 0.0017426764024188349 0.0 0.0 1.2391221346835377 0.0 74-75 0.0017426764024188349 0.0 0.0 1.4396883460891965 0.0 76-77 0.0017426764024188349 0.0 0.0 1.5481303458578957 0.0 78-79 0.0017426764024188349 0.0 0.0 1.6689295055710196 0.0 80-81 0.001980314093657767 0.0 0.0 1.9129042019096565 0.0 82-83 0.0020595266574040777 0.0 0.0 2.1064204951418937 0.0 84-85 0.0021387392211503885 0.0 0.0 2.4488564082171944 0.0 86-87 0.002217951784896699 0.0 0.0 2.927617143499896 0.0 88-89 0.0022971643486430094 0.0 0.0 3.5514952955658394 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8830 0.0 32.890156 1 CTACACG 110 2.7166006E-6 30.247593 4 GATCGGT 65 0.0058297184 29.24346 6 GGTATCA 5770 0.0 28.420305 1 ATCAACG 10375 0.0 27.809042 3 TATCAAC 10400 0.0 27.742195 2 TCAACGC 10445 0.0 27.53166 4 CAACGCA 10535 0.0 27.337238 5 GCGTAAG 70 0.008361291 27.161104 2 AACGCAG 10685 0.0 26.995806 6 GAGTACT 5685 0.0 25.91274 12-13 GTACATG 5350 0.0 25.498438 1 CGCAGTA 95 0.0012211466 25.016806 2 ACGCAGA 11600 0.0 24.825428 7 CGCAGAG 11660 0.0 24.699642 8 TACATGG 5425 0.0 24.620226 2 GTACTTT 6100 0.0 24.07192 14-15 GCAGAGT 11605 0.0 24.03862 9 AGTACTT 5725 0.0 23.407537 12-13 ACATGGG 5460 0.0 23.156582 3 >>END_MODULE