FastQCFastQC Report
Thu 26 May 2016
SRR936975_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936975_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631213
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99051.5692008878144144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75061.189139006959616No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56020.8874975642136648No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA29860.4730574306929674No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21670.34330725127651046No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17620.27914507464199884No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16260.2575992573030023No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14670.23240966203167554No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT14070.22290415438211822No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12890.2042099893379889No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA9790.1550981998152763No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG9260.14670166805816737No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT8670.13735458553610272No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG8330.1319681312013536No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7860.12452215020920038No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7800.12357159944424465No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC6830.10820436207746038No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6580.10424373389014485No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGC1004.9359583E-528.5115551
GTATCAA101950.027.5933321
GGTATCA71800.027.4658681
CGTCACT950.001223048125.0101361
GAGTACT64650.023.546312-13
AGTACTT64950.023.51067212-13
TACCTGG4300.023.196052
GTACATG59450.022.7005371
ATCAACG125700.022.4069423
TATCAAC126100.022.3735332
TCAACGC126600.022.2476524
TAGTCGT753.0314483E-522.16862514-15
TACATGG59800.022.0803952
GTGTAAG2806.548362E-1122.0625131
GTACTTT68550.021.96765914-15
CAACGCA128050.021.9586355
AACGCAG130250.021.587746
GTATAGA1353.782958E-421.119671
GTCGGGA2257.144081E-821.1096342
TACTTTT73250.020.72026314-15
Produced by FastQC (version 0.11.5)