FastQCFastQC Report
Thu 26 May 2016
SRR936974_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936974_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442966
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67711.5285597540217533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47241.066447537734273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33350.7528794535020746No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17460.3941611771558088No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT13720.30973031790250266No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13030.2941535016231494No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10620.2397475201256981No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9870.22281619808292283No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8150.18398703286482485No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT8030.1812780213379808No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA7970.17992351557455877No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG7880.17789175692942574No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7350.16592695601919785No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC6670.15057589070041494No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG4960.11197247644288727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59550.032.1654431
CGAATTA300.003955803331.65793670-71
GTCTATA953.4711346E-530.0188431
GGTATCA39950.029.2674331
CTGGACG1056.861703E-527.1629734
TATCAAC73050.026.3512422
ATCAACG73900.025.7265663
TCAACGC74750.025.43694
CAACGCA75150.025.2382535
GTACATG39350.025.1237751
AACGCAG76700.024.7282246
TACATGG39650.024.5740622
GAGTACT40050.024.56459412-13
CATGGGG9850.024.1295474
AGTACTT40800.023.93830712-13
GGACGCT1000.00164493523.7676036
ACATGGG39550.023.6747843
CGCAGAG81900.022.9866528
ACGCAGA82400.022.8471727
GTACTTT42950.022.79532614-15
Produced by FastQC (version 0.11.5)