##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936974_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 442966 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.915697818794218 33.0 31.0 34.0 30.0 34.0 2 31.981082069504208 33.0 31.0 34.0 30.0 34.0 3 31.95757913699923 33.0 31.0 34.0 30.0 34.0 4 35.56035677681808 37.0 35.0 37.0 33.0 37.0 5 35.579909067513086 37.0 35.0 37.0 33.0 37.0 6 35.683404595386556 37.0 35.0 37.0 33.0 37.0 7 35.640809904146145 37.0 35.0 37.0 33.0 37.0 8 35.59624440701995 37.0 35.0 37.0 33.0 37.0 9 37.22064221633263 39.0 37.0 39.0 34.0 39.0 10-11 37.06803118072267 39.0 37.0 39.0 33.0 39.0 12-13 36.999099253667325 39.0 37.0 39.0 33.0 39.0 14-15 38.056921975953 40.0 38.0 41.0 33.0 41.0 16-17 38.035195703507725 40.0 38.0 41.0 33.0 41.0 18-19 38.13205979691443 40.0 38.0 41.0 33.5 41.0 20-21 38.13574292383614 40.0 38.0 41.0 33.5 41.0 22-23 38.02506964417134 40.0 38.0 41.0 33.0 41.0 24-25 37.864429775648695 40.0 37.5 41.0 33.0 41.0 26-27 37.87689461493659 40.0 37.5 41.0 33.0 41.0 28-29 37.78943756405683 40.0 37.0 41.0 33.0 41.0 30-31 37.522694744066136 40.0 37.0 41.0 33.0 41.0 32-33 37.19475761119364 39.0 35.5 41.0 32.0 41.0 34-35 36.961824383812754 39.0 35.0 41.0 31.5 41.0 36-37 36.70623253251942 39.0 35.0 41.0 31.0 41.0 38-39 36.484403994889 39.0 35.0 41.0 30.5 41.0 40-41 36.215477034354784 39.0 35.0 41.0 30.0 41.0 42-43 35.872936071842986 39.0 35.0 41.0 29.5 41.0 44-45 35.40030837581214 38.5 35.0 40.0 27.5 41.0 46-47 34.734529286672114 38.0 35.0 40.0 23.5 41.0 48-49 34.14608796160428 38.0 34.0 40.0 19.0 41.0 50-51 33.369594957626546 37.5 33.5 39.5 15.0 40.5 52-53 33.52433933981389 38.0 33.5 40.0 14.5 40.5 54-55 33.85760193784624 38.0 34.0 40.0 11.5 41.0 56-57 33.73949355029505 38.0 34.0 40.0 8.5 41.0 58-59 33.49498494241093 38.0 34.0 40.0 7.5 41.0 60-61 33.211567027717706 38.0 33.5 40.0 3.5 41.0 62-63 32.895497622842385 37.0 33.0 40.0 2.0 41.0 64-65 32.54716614819196 37.0 33.0 40.0 2.0 41.0 66-67 32.16282626657576 36.0 32.5 39.5 2.0 41.0 68-69 31.79077739600782 36.0 32.0 39.0 2.0 41.0 70-71 31.35313771260097 35.0 31.5 39.0 2.0 40.5 72-73 30.948018583819074 35.0 31.0 38.0 2.0 40.0 74-75 30.513357684336945 35.0 31.0 37.0 2.0 39.5 76-77 30.067090927971897 35.0 30.5 37.0 2.0 39.0 78-79 29.63911225692265 35.0 30.0 36.0 2.0 39.0 80-81 29.240712605482138 35.0 29.5 36.0 2.0 37.5 82-83 28.835697322142103 34.0 29.0 35.0 2.0 37.0 84-85 28.504226057981874 34.0 29.0 35.0 2.0 37.0 86-87 28.169917104247276 34.0 29.0 35.0 2.0 36.0 88-89 27.80412153528713 34.0 27.5 35.0 2.0 36.0 90-91 27.43349038075157 34.0 27.0 35.0 2.0 35.5 92-93 27.11113832664358 34.0 26.0 35.0 2.0 35.0 94-95 26.730778434462238 34.0 25.0 35.0 2.0 35.0 96-97 26.275136240704704 34.0 24.5 35.0 2.0 35.0 98-99 25.722623181011635 33.0 21.5 35.0 2.0 35.0 100-101 23.755863881200817 31.0 8.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1952901434168517 1101 2 0.05983535468104151 1101 3 0.23989562498453054 1101 4 0.06124557786053231 1101 5 0.10480563649015551 1101 6 -0.06584390174386101 1101 7 -0.06364788051902792 1101 8 0.03914523288606375 1101 9 -0.011159836882249863 1101 10-11 -0.06131142228193909 1101 12-13 -0.013766618426124921 1101 14-15 0.16612904060916378 1101 16-17 0.2141444804767687 1101 18-19 0.21759387857861867 1101 20-21 0.16670553652300413 1101 22-23 0.03843402550496222 1101 24-25 0.1381918188542457 1101 26-27 0.16138355168954632 1101 28-29 0.22729418708211568 1101 30-31 0.21328345384055325 1101 32-33 0.17307581505745873 1101 34-35 -0.12742907467277576 1101 36-37 0.003447083167465337 1101 38-39 0.25063318636265564 1101 40-41 0.3616722072365164 1101 42-43 0.4755606027019823 1101 44-45 0.4021586252701539 1101 46-47 0.6044549451746306 1101 48-49 0.6532839551565033 1101 50-51 0.5112813521419994 1101 52-53 0.6628298107049488 1101 54-55 0.7021920364725958 1101 56-57 0.5788655529419131 1101 58-59 0.4980810998093119 1101 60-61 0.6428087521641999 1101 62-63 0.6062591938751893 1101 64-65 0.678142494960781 1101 66-67 0.6651299861569946 1101 68-69 0.4779619669333748 1101 70-71 0.5188514957359196 1101 72-73 0.4653630752119682 1101 74-75 0.33314546652706767 1101 76-77 0.4598788181039062 1101 78-79 0.6478390496541628 1101 80-81 0.4821158716169833 1101 82-83 0.4700367975917281 1101 84-85 0.5599748194101188 1101 86-87 0.6217121049427838 1101 88-89 0.707307357480957 1101 90-91 0.9667251144933928 1101 92-93 0.9670661867180641 1101 94-95 0.7569449924638931 1101 96-97 0.6245265770551605 1101 98-99 0.41380744023149774 1101 100-101 0.5630415546590406 1102 1 0.3090468141234375 1102 2 0.26405530986164294 1102 3 0.1575189913475974 1102 4 0.277532542562561 1102 5 0.17425955883333444 1102 6 0.2711400670332509 1102 7 0.14462885451049345 1102 8 0.11030194793845283 1102 9 0.21803369379707505 1102 10-11 0.3285048655438487 1102 12-13 0.35414370402813944 1102 14-15 0.4203408154960968 1102 16-17 0.462051509286475 1102 18-19 0.30504014158367454 1102 20-21 0.3701311421338218 1102 22-23 0.16894469110102506 1102 24-25 0.39458374964033993 1102 26-27 0.26177566760861026 1102 28-29 0.4587938071290054 1102 30-31 0.39711456521956023 1102 32-33 0.34829017104231497 1102 34-35 0.4924579726932734 1102 36-37 0.24105232372190954 1102 38-39 0.27042842069577944 1102 40-41 0.16914415205827993 1102 42-43 0.09395959006773325 1102 44-45 0.1553500161568806 1102 46-47 0.509653414168092 1102 48-49 0.6705965779954539 1102 50-51 0.7423122198343606 1102 52-53 0.6733630151627779 1102 54-55 0.5437795731493722 1102 56-57 0.8570977914792763 1102 58-59 0.9851024180851695 1102 60-61 1.0034854346676312 1102 62-63 0.9154904400137696 1102 64-65 0.8719078195569878 1102 66-67 0.8011930260832862 1102 68-69 0.7795080424374881 1102 70-71 0.7492844485420846 1102 72-73 0.8271855370067662 1102 74-75 0.8230481856136329 1102 76-77 0.9176227576902853 1102 78-79 1.0781036606273808 1102 80-81 1.0862154903722434 1102 82-83 1.0392167877323182 1102 84-85 1.2417221086114871 1102 86-87 0.950719211034734 1102 88-89 1.181680434842857 1102 90-91 1.0567826133470746 1102 92-93 1.1535053048824047 1102 94-95 1.1606193435836225 1102 96-97 1.2426279524208503 1102 98-99 1.4886027714365184 1102 100-101 1.350440565704588 1103 1 0.06502924343245198 1103 2 0.28623428252818073 1103 3 0.2234493295373987 1103 4 0.20095399766415767 1103 5 0.18932631136027567 1103 6 0.05566911961883392 1103 7 0.03296642336161426 1103 8 0.16916031299025747 1103 9 0.03717804254309698 1103 10-11 0.17828436947080917 1103 12-13 0.005412059535650826 1103 14-15 0.015138315582341022 1103 16-17 0.2229124501463815 1103 18-19 0.17717541750560883 1103 20-21 0.22909414938978756 1103 22-23 0.17644356993454124 1103 24-25 0.17194596115558625 1103 26-27 0.402781037708543 1103 28-29 0.46909703953819104 1103 30-31 0.30527090757205855 1103 32-33 0.4786358352903264 1103 34-35 0.5943950033844914 1103 36-37 0.46044160422051306 1103 38-39 0.46851410081261236 1103 40-41 0.5378210606285947 1103 42-43 0.7347191659793353 1103 44-45 0.8125188695772962 1103 46-47 1.0513076234295013 1103 48-49 1.054988503297018 1103 50-51 0.8561997352144601 1103 52-53 0.9459185847728406 1103 54-55 1.0775654935983 1103 56-57 1.1308032754207247 1103 58-59 1.2517689889256403 1103 60-61 1.3055190581626803 1103 62-63 1.3365746942368304 1103 64-65 1.2773733623740426 1103 66-67 1.361674207735252 1103 68-69 1.2543551038314114 1103 70-71 1.354008584093993 1103 72-73 1.1550261128838635 1103 74-75 0.983073138754623 1103 76-77 1.0666142828216643 1103 78-79 1.1296341966024528 1103 80-81 1.315718267415356 1103 82-83 1.4633612514697738 1103 84-85 1.395120680417886 1103 86-87 1.2376817698241034 1103 88-89 1.0890490858783544 1103 90-91 1.247129370013706 1103 92-93 1.3529809439472942 1103 94-95 1.2214742473932922 1103 96-97 1.1478103288338097 1103 98-99 1.2731045135567314 1103 100-101 1.5116959271801598 1104 1 0.3457543552597606 1104 2 0.31840036333609234 1104 3 0.24536829431281504 1104 4 0.265521585197952 1104 5 0.4384211392964019 1104 6 0.336893496754918 1104 7 0.4963698703217787 1104 8 0.42777162479971054 1104 9 0.4340959925516188 1104 10-11 0.44551003739037043 1104 12-13 0.3648506093119934 1104 14-15 0.5533716876121204 1104 16-17 0.7181004255646641 1104 18-19 0.6547774827591866 1104 20-21 0.3964413863824987 1104 22-23 0.4134068597276155 1104 24-25 0.6191598171885815 1104 26-27 0.4010153433238557 1104 28-29 0.4167046251236357 1104 30-31 0.6195178287693182 1104 32-33 0.8583676932879101 1104 34-35 0.8022354982660005 1104 36-37 0.8197076436259394 1104 38-39 0.9625482862795209 1104 40-41 0.9367705739463972 1104 42-43 0.8409642443599239 1104 44-45 1.093155696685379 1104 46-47 1.3667631319900764 1104 48-49 1.2906386847373383 1104 50-51 1.2078042846173744 1104 52-53 1.2771973534141097 1104 54-55 1.4593664612800055 1104 56-57 1.4747138622807157 1104 58-59 1.4359014850086353 1104 60-61 1.4590959084886137 1104 62-63 1.3789899206110263 1104 64-65 1.4816256415404823 1104 66-67 1.6426032877070007 1104 68-69 1.3364020510067114 1104 70-71 1.3484912965252853 1104 72-73 1.448360718058364 1104 74-75 1.5424024996138463 1104 76-77 1.3834650958528947 1104 78-79 1.4230804261346037 1104 80-81 1.207761277295127 1104 82-83 1.3447725486665796 1104 84-85 1.34781427764473 1104 86-87 1.4111632726532726 1104 88-89 1.5850955540836793 1104 90-91 1.5537604657184012 1104 92-93 1.806855729831483 1104 94-95 1.7527929978499373 1104 96-97 1.887255738434316 1104 98-99 1.6900062678857246 1104 100-101 1.4378046614817173 1105 1 0.3004206377714489 1105 2 0.2567263394155894 1105 3 0.2466545275017431 1105 4 0.3102104711142104 1105 5 0.22513966688524079 1105 6 0.2697384044719726 1105 7 0.19367975648157199 1105 8 0.3606165325167652 1105 9 0.4038815174548134 1105 10-11 0.4025172643059207 1105 12-13 0.29299647844548815 1105 14-15 0.5255153066950129 1105 16-17 0.39163557746023514 1105 18-19 0.31912721470818894 1105 20-21 0.4811612790935058 1105 22-23 0.2100070881626337 1105 24-25 0.24992342810818258 1105 26-27 0.24689545267082025 1105 28-29 0.40031629931905144 1105 30-31 0.09214850148642739 1105 32-33 -0.047884771992372066 1105 34-35 0.12370290168703946 1105 36-37 -0.03508005929877811 1105 38-39 -0.15368260458273397 1105 40-41 -0.06936561585360579 1105 42-43 0.20432169027810687 1105 44-45 0.6448399761718449 1105 46-47 0.6380537212220716 1105 48-49 0.7849826886612092 1105 50-51 0.44623590331612206 1105 52-53 0.6933846838457001 1105 54-55 0.9348628615420509 1105 56-57 0.8960066809835681 1105 58-59 0.5738371321897304 1105 60-61 0.8467202872379929 1105 62-63 1.0944271198468343 1105 64-65 0.9855906697175598 1105 66-67 1.2590605556105068 1105 68-69 1.1623198212614518 1105 70-71 0.9857220879188446 1105 72-73 1.0537580955146382 1105 74-75 1.1343630276897443 1105 76-77 1.234683930422726 1105 78-79 1.2101162245767192 1105 80-81 0.8990996115030967 1105 82-83 0.9246339098785903 1105 84-85 1.1100034808318462 1105 86-87 1.155456684738379 1105 88-89 0.9952787479893317 1105 90-91 1.1135754539365053 1105 92-93 1.0660179975806443 1105 94-95 1.3689192750794916 1105 96-97 1.5286436354945003 1105 98-99 1.402390807019799 1105 100-101 1.5718267298132815 1106 1 0.28451998249948574 1106 2 0.15854246613205092 1106 3 0.0529445273490019 1106 4 0.13521161669456205 1106 5 0.235037481117935 1106 6 0.1340405932941593 1106 7 0.29006125001286165 1106 8 0.2704822704996275 1106 9 0.2892929335448713 1106 10-11 0.22547927686147773 1106 12-13 0.2734910636690273 1106 14-15 0.4894448000430103 1106 16-17 0.6074400039827097 1106 18-19 0.219858858692362 1106 20-21 0.4292435775859218 1106 22-23 0.3968817922210377 1106 24-25 0.7075827783350093 1106 26-27 0.5068505459210684 1106 28-29 0.4635446974922317 1106 30-31 0.8574954595887334 1106 32-33 1.0152367053480518 1106 34-35 0.8133193613855241 1106 36-37 0.645211376342516 1106 38-39 0.6872802938882998 1106 40-41 0.6879240937799622 1106 42-43 0.9465050136830726 1106 44-45 0.9147310696664732 1106 46-47 1.3297245883500253 1106 48-49 1.6686910086294375 1106 50-51 1.787878512795416 1106 52-53 1.8115627528554583 1106 54-55 1.7190507978172391 1106 56-57 1.9447320319130128 1106 58-59 1.9832064520204895 1106 60-61 1.788837930702833 1106 62-63 1.7427874125044198 1106 64-65 1.8759357185192798 1106 66-67 2.1462972630598394 1106 68-69 2.104865148057339 1106 70-71 2.014079020207408 1106 72-73 1.787700877594709 1106 74-75 1.6510503148027276 1106 76-77 1.9470898019038856 1106 78-79 2.1068765233440736 1106 80-81 1.8508248555117106 1106 82-83 1.9147895560622779 1106 84-85 2.1309370524458906 1106 86-87 1.9484885013233288 1106 88-89 1.7148941384794902 1106 90-91 1.8338323694232237 1106 92-93 1.8788989030473502 1106 94-95 2.0025092184114506 1106 96-97 1.984004187816975 1106 98-99 2.0311769500798746 1106 100-101 2.129961114025658 1107 1 0.052838660890110134 1107 2 0.04402455665011118 1107 3 0.05469550751724839 1107 4 0.016087868074407652 1107 5 -0.11584371868412546 1107 6 0.12715086697997435 1107 7 0.13194012786296128 1107 8 0.29739407438984955 1107 9 0.04537378315090024 1107 10-11 0.2617074371903598 1107 12-13 0.1790713613356516 1107 14-15 0.3212555419256944 1107 16-17 0.09683896100757039 1107 18-19 0.3489270327245606 1107 20-21 -0.08749541217465406 1107 22-23 0.008982587775072659 1107 24-25 0.12715188495447904 1107 26-27 0.023855989185499027 1107 28-29 0.04432769014572813 1107 30-31 -0.2598297278803372 1107 32-33 -0.20728051896773536 1107 34-35 -0.10775330429145669 1107 36-37 -0.15881207156581922 1107 38-39 -0.3329701724053322 1107 40-41 0.203045330124219 1107 42-43 -0.05079238649202722 1107 44-45 0.14533706106383448 1107 46-47 0.2531667869720806 1107 48-49 0.46475891620813314 1107 50-51 0.4521756465170057 1107 52-53 0.4818455742174166 1107 54-55 0.6159618404117353 1107 56-57 0.8160349895469139 1107 58-59 0.9641835750839434 1107 60-61 0.9167165801642128 1107 62-63 1.058544330779334 1107 64-65 0.8138520521190671 1107 66-67 1.0332829126354177 1107 68-69 0.8917118611630421 1107 70-71 0.659600698103187 1107 72-73 0.7855597333672328 1107 74-75 0.8983572311743906 1107 76-77 0.5216872667891188 1107 78-79 0.3671444994393873 1107 80-81 0.3406710737751979 1107 82-83 0.3260510230791418 1107 84-85 0.38618047675406686 1107 86-87 0.6095310958191611 1107 88-89 0.8840159838603299 1107 90-91 0.7222264160906491 1107 92-93 0.8945911111856049 1107 94-95 0.7381016968096858 1107 96-97 0.6789149258931779 1107 98-99 0.5163411996326133 1107 100-101 0.7485614718346483 1108 1 -0.024847384288197105 1108 2 -0.16487186741659343 1108 3 -0.31434801632503806 1108 4 -0.23572630788837046 1108 5 -0.347681483029632 1108 6 -0.5586485990052523 1108 7 -0.5845406164815614 1108 8 -0.20770855145581635 1108 9 -0.24535303389171048 1108 10-11 -0.3393171831841073 1108 12-13 -0.12179518411293344 1108 14-15 -0.21053144187366968 1108 16-17 -0.1318373048204009 1108 18-19 -0.06405394914186502 1108 20-21 -0.27288945283185484 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2316 48-49 -0.9383791972748057 2316 50-51 -1.3017105056450085 2316 52-53 -1.4310900214767983 2316 54-55 -1.4965450014054547 2316 56-57 -1.7031220985756939 2316 58-59 -1.446918413604255 2316 60-61 -1.4044474409998031 2316 62-63 -1.565547988140576 2316 64-65 -1.7959819197838698 2316 66-67 -1.782736062004247 2316 68-69 -1.801601402839875 2316 70-71 -1.8425157040132163 2316 72-73 -2.613208739539168 2316 74-75 -2.5316008056801955 2316 76-77 -2.846046723358665 2316 78-79 -2.776551906325981 2316 80-81 -3.189223106118984 2316 82-83 -2.9502397997634056 2316 84-85 -2.809185332404848 2316 86-87 -2.78869003171231 2316 88-89 -2.759388504371749 2316 90-91 -2.8222910077371175 2316 92-93 -2.817933975477416 2316 94-95 -3.405859259220236 2316 96-97 -3.1749803486917507 2316 98-99 -3.0405918417831863 2316 100-101 -2.6814421771345174 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 882.0 3 288.0 4 289.0 5 291.0 6 370.0 7 390.0 8 521.0 9 695.0 10 864.0 11 1273.0 12 1962.0 13 2769.0 14 3269.0 15 4443.0 16 6291.0 17 8806.0 18 7426.0 19 3919.0 20 3353.0 21 3108.0 22 3285.0 23 3498.0 24 3920.0 25 4275.0 26 4690.0 27 5455.0 28 6472.0 29 7434.0 30 9228.0 31 11381.0 32 14326.0 33 18837.0 34 26046.0 35 38910.0 36 56394.0 37 82657.0 38 78651.0 39 16286.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.11564424590494 18.708685175105977 15.324454762878029 19.851215816111058 2 15.037626248938516 21.19740003974922 41.56119572876579 22.20377798254648 3 18.66746752894846 28.374686128222265 31.232048340769914 21.72579800205936 4 11.607001693958216 17.26482213438735 38.88424618859401 32.24392998306042 5 12.02551949430917 38.66578408736631 33.53500119735588 15.773695220968639 6 27.60573497753492 37.62319652485842 18.723612846096678 16.047455651509985 7 25.330950788860175 36.479361321453744 20.96985524009181 17.219832649594274 8 26.202809888602985 32.41221639621282 20.428509141513292 20.956464573670903 9 27.94782416182281 16.111119896443522 21.013787198666236 34.92726874306743 10-11 23.8186843909663 27.424392525159192 26.946342499347654 21.81058058452686 12-13 26.87693454734416 25.823750028241566 27.59980569801857 19.699509726395696 14-15 23.59812265967841 25.336187372570723 29.091319842538365 21.9743701252125 16-17 19.611512757755683 29.25208638521408 29.173815173772255 21.96258568325798 18-19 19.33206404271321 26.411634110039817 34.555985428001634 19.700316419245336 20-21 21.260779805116485 24.926478228068827 34.26216214995279 19.550579816861894 22-23 20.35419312953397 25.475258983261355 34.049397352301916 20.121150534902757 24-25 19.82707137849557 25.44956587216739 34.818056918104446 19.905305831232596 26-27 19.94504644749215 25.465349798326788 34.670972942035746 19.91863081214532 28-29 19.310909285903783 25.50787137123859 35.1070495199282 20.074169822929424 30-31 19.506052426897316 25.95234144199367 34.825132670236144 19.716473460872862 32-33 19.06126181042365 26.12471355842279 34.29160034768081 20.522424283472745 34-35 19.64644725776501 25.837345419448788 34.28614969549537 20.23005762729084 36-37 20.076713640037386 25.609780876224193 34.176626496232046 20.136878987506375 38-39 20.04501809528755 25.74418704409019 34.15335953744697 20.057435323175287 40-41 19.73195105899989 25.915386117579857 34.21704672061661 20.135616102803645 42-43 19.447209974873182 26.532837569674435 33.45287197513054 20.567080480321838 44-45 19.51862633660036 26.89895171476528 32.813799844907514 20.768622103726848 46-47 19.834566148143214 26.994072781413074 31.7308810233059 21.44048004713781 48-49 20.17439301073448 27.31818540968248 31.057419886447015 21.450001693136027 50-51 20.696498094005005 27.66384953419672 30.601350733556913 21.03830163824136 52-53 20.355641545470554 27.946973220211945 30.486786199672196 21.210599034645305 54-55 19.79173251456179 27.831986273535918 30.747279541247117 21.629001670655168 56-57 20.33634810764675 28.421257037829 29.642370468383255 21.600024386140994 58-59 20.381412087155393 27.631944334696218 30.142482045817314 21.844161532331075 60-61 20.437103318408738 27.269792026516566 30.767589462917677 21.525515192157023 62-63 21.147237850969482 27.203239440435656 30.49144758825601 21.158075120338847 64-65 20.84940995324328 27.36735518624797 30.85435428994584 20.92888057056291 66-67 20.428347423808145 27.757012405412816 30.486338692944305 21.32830147783474 68-69 20.46990284685254 27.98744517141518 30.452685118802325 21.08996686292995 70-71 20.344754010821966 27.96698092439413 30.044559043837655 21.643706020946247 72-73 20.265972759241592 27.86369460547186 29.617127923188512 22.253204712098036 74-75 20.303932426345327 27.95033580231861 30.017983966836347 21.727747804499717 76-77 20.405942136649273 27.677698472015557 30.127508913430773 21.7888504779044 78-79 20.09159901942075 27.624805357729628 30.11543599303974 22.16815962980988 80-81 19.900572784763675 28.361031667443854 29.99391423245374 21.74448131533873 82-83 19.960481448862996 28.300928121400986 29.58923288846736 22.149357541268657 84-85 19.91693093797593 27.76084961203203 29.203663709161194 23.11855574083085 86-87 20.075754154624278 27.703666907514453 29.5368948699422 22.683684067919074 88-89 20.553722908241944 26.95684962282086 29.64124517660022 22.848182292336976 90-91 19.891644012535785 27.16124924360792 30.334257561662888 22.6128491821934 92-93 19.647935905411707 27.54493384261554 29.947013123086947 22.860117128885808 94-95 19.383211670591635 27.85380601547559 29.70324526802306 23.059737045909717 96-97 19.176850922370058 27.73320656414472 29.34669077087937 23.743251742605853 98-99 19.44896829788916 27.895711688106346 29.128446065753362 23.526873948251133 100-101 19.807416443586007 28.168253645533305 28.98919047134967 23.035139439531022 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 617.0 1 435.5 2 341.5 3 417.0 4 376.5 5 387.0 6 655.5 7 1480.0 8 2534.0 9 2799.5 10 2205.0 11 1693.0 12 1635.5 13 1672.5 14 1727.0 15 1766.0 16 1688.0 17 1740.5 18 1835.0 19 1861.5 20 1914.0 21 2079.5 22 2330.0 23 2616.5 24 2910.0 25 3274.0 26 3877.5 27 4499.5 28 4986.5 29 5610.0 30 6259.0 31 6992.5 32 7961.5 33 8819.0 34 9628.0 35 10196.0 36 10986.0 37 12137.5 38 12443.5 39 12122.0 40 12082.0 41 12358.5 42 12605.5 43 13032.5 44 13673.0 45 14107.5 46 14272.0 47 15086.0 48 20463.5 49 20439.5 50 14839.0 51 13222.5 52 12225.5 53 11701.5 54 11319.0 55 10574.0 56 9534.5 57 8563.5 58 7932.0 59 7187.0 60 6387.0 61 5691.5 62 4697.0 63 3392.5 64 2250.5 65 1685.5 66 1250.5 67 890.5 68 663.0 69 427.0 70 301.0 71 234.0 72 167.5 73 141.0 74 115.5 75 85.0 76 61.5 77 36.0 78 21.0 79 13.5 80 7.5 81 4.5 82 4.0 83 3.5 84 2.5 85 2.5 86 1.5 87 0.0 88 1.5 89 2.0 90 0.5 91 0.0 92 0.5 93 1.0 94 1.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03995792002094969 2 0.042892682508364074 3 0.0248326056627371 4 0.04876220748319284 5 0.07314331122478926 6 0.06298451799912408 7 0.06817679009224184 8 0.07111155257965622 9 0.06975704681623421 10-11 0.0755136963107778 12-13 0.08036734196304005 14-15 0.0719016809416524 16-17 0.061968638676557566 18-19 0.044924441153497106 20-21 0.05418023053688093 22-23 0.02900899843328833 24-25 0.015012438877927425 26-27 0.010497419666520682 28-29 0.014335185996216415 30-31 0.010045917745380006 32-33 0.006998279777680454 34-35 0.007562657179106297 36-37 0.004515019211406745 38-39 0.0074497816988211285 40-41 0.0055308985339732625 42-43 0.0029347624874143836 44-45 0.0010158793225665175 46-47 0.0021446341254182035 48-49 2.2575096057033723E-4 50-51 0.0048536456522622505 52-53 0.004515019211406745 54-55 0.005869524974828767 56-57 0.020656212892185857 58-59 0.0074497816988211285 60-61 0.016479820121634618 62-63 0.011287548028516862 64-65 0.007901283619961803 66-67 0.01230342735108338 68-69 0.02449397922188159 70-71 0.015915442720208776 72-73 0.014335185996216415 74-75 0.016141193680779112 76-77 0.021446341254182037 78-79 0.03758753493496115 80-81 0.02923474939385867 82-83 0.030702130637565862 84-85 0.018398703286482482 86-87 0.019414582609049002 88-89 0.016479820121634618 90-91 0.016705571082204954 92-93 0.027202990748725637 94-95 0.016931322042775292 96-97 0.03758753493496115 98-99 0.05666349110315464 100-101 0.06795103913167151 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 442966.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.73314150351739 #Duplication Level Percentage of deduplicated Percentage of total 1 83.62738317032844 53.29835845163411 2 10.078674874745769 12.846912239202348 3 2.813244396063206 5.378907095348208 4 1.1385444185393654 2.902520501392372 5 0.5732345734574239 1.8267020092435213 6 0.3662038513914059 1.4003593127916913 7 0.24041276604304856 1.0725582586231548 8 0.18963515049619195 0.9668835104491695 9 0.13806518918384209 0.7919395415067335 >10 0.7634836788850503 8.868057103793799 >50 0.04210464567093496 1.8139296649852847 >100 0.024059797479054745 2.9912701354559106 >500 0.0024767438581379886 1.2610013266090803 >1k 0.0021229233069754187 3.053733899309758 >5k 3.5382055116256976E-4 1.526866949654879 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6771 1.5285597540217533 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4724 1.066447537734273 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3335 0.7528794535020746 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1746 0.3941611771558088 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1372 0.30973031790250266 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1303 0.2941535016231494 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1062 0.2397475201256981 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 987 0.22281619808292283 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 815 0.18398703286482485 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 803 0.1812780213379808 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 797 0.17992351557455877 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 788 0.17789175692942574 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 735 0.16592695601919785 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 667 0.15057589070041494 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 496 0.11197247644288727 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.386264408555058E-4 0.0 22-23 0.0 0.0 0.0 9.030038422813489E-4 0.0 24-25 0.0 0.0 0.0 0.0019188831648478664 0.0 26-27 0.0 0.0 0.0 0.002821887007129215 0.0 28-29 0.0 0.0 0.0 0.005418023053688093 0.0 30-31 0.0 0.0 0.0 0.010271668705950344 0.0 32-33 0.0 0.0 0.0 0.015915442720208773 0.0 34-35 0.0 0.0 0.0 0.0212205902936117 0.0 36-37 0.0 0.0 0.0 0.027428741709295972 0.0 38-39 0.0 0.0 0.0 0.03612015369125396 0.0 40-41 0.0 0.0 0.0 0.043344184429504745 0.0 42-43 1.1287548028516862E-4 0.0 0.0 0.06208151415684274 0.0 44-45 2.2575096057033723E-4 0.0 0.0 0.08318922897016927 0.0 46-47 0.0013545057634220233 0.0 0.0 0.09876604524952254 0.0 48-49 0.0018060076845626979 0.0 0.0 0.12179264322769692 0.0 50-51 0.0018060076845626979 0.0 0.0 0.15734841951752504 0.0 52-53 0.0018060076845626979 0.0 0.0 0.2268797153731889 0.0 54-55 0.0018060076845626979 0.0 0.0 0.27304578680982283 0.0 56-57 0.0018060076845626979 0.0 0.0 0.35871827634626585 0.0 58-59 0.0018060076845626979 0.0 0.0 0.4633538465706172 0.0 60-61 0.0018060076845626979 0.0 0.0 0.5767937042572117 0.0 62-63 0.0018060076845626979 0.0 0.0 0.743059286717265 0.0 64-65 0.0018060076845626979 0.0 0.0 0.8159768469814839 0.0 66-67 0.0018060076845626979 0.0 0.0 1.009558295670548 0.0 68-69 0.0018060076845626979 0.0 0.0 1.2514504499216645 0.0 70-71 0.0018060076845626979 0.0 0.0 1.3972855704501024 0.0 72-73 0.0018060076845626979 0.0 0.0 1.704194001345476 0.0 74-75 0.0018060076845626979 0.0 0.0 1.9952998650009255 0.0 76-77 0.0018060076845626979 0.0 0.0 2.140119106206797 0.0 78-79 0.0018060076845626979 0.0 0.0 2.2976932766848925 0.0 80-81 0.0024832605662737095 0.0 0.0 2.620742901261045 0.0 82-83 0.0024832605662737095 0.0 0.0 2.8748256073829594 0.0 84-85 0.0024832605662737095 0.0 0.0 3.3340030611830254 0.0 86-87 0.0024832605662737095 0.0 0.0 3.983262823783315 0.0 88-89 0.0024832605662737095 0.0 0.0 4.817525498570996 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5955 0.0 32.165443 1 CGAATTA 30 0.0039558033 31.657936 70-71 GTCTATA 95 3.4711346E-5 30.018843 1 GGTATCA 3995 0.0 29.267433 1 CTGGACG 105 6.861703E-5 27.162973 4 TATCAAC 7305 0.0 26.351242 2 ATCAACG 7390 0.0 25.726566 3 TCAACGC 7475 0.0 25.4369 4 CAACGCA 7515 0.0 25.238253 5 GTACATG 3935 0.0 25.123775 1 AACGCAG 7670 0.0 24.728224 6 TACATGG 3965 0.0 24.574062 2 GAGTACT 4005 0.0 24.564594 12-13 CATGGGG 985 0.0 24.129547 4 AGTACTT 4080 0.0 23.938307 12-13 GGACGCT 100 0.001644935 23.767603 6 ACATGGG 3955 0.0 23.674784 3 CGCAGAG 8190 0.0 22.986652 8 ACGCAGA 8240 0.0 22.847172 7 GTACTTT 4295 0.0 22.795326 14-15 >>END_MODULE