FastQCFastQC Report
Thu 26 May 2016
SRR936974_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936974_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442966
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69141.5608421413833116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51891.17142173439948No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39760.8975858192276608No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23310.526225489089456No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA17320.39100066370782405No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12460.2812856968706402No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11620.2623226161827319No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10740.2424565316525422No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10350.23365224419029906No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT10270.23184623650573633No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG7270.16412094833463517No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA7240.16344369545292414No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG6410.14470636572558618No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT5950.13432182153935066No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5400.1219055187079821No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5170.11671324661486435No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5110.11535874085144232No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT5110.11535874085144232No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG4860.10971496683718389No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4730.10678020434976951No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA200.002070857771.2705842
CTAAGGG1351.1903467E-831.6758172
ATATGGG1303.0280717E-729.232613
GGTAGTT1354.2254032E-728.140397
TAAGGGT1658.38063E-825.9107233
AGGGTAG2059.549694E-1025.4836545
GTATCAA81350.025.0008241
GTAAGGT1351.3419971E-524.6256224
AGTACTT45400.024.26643612-13
GTGTAGG2402.2737368E-1023.7595441
GTATAGG1201.703854E-423.7595441
CATATAG1000.001651440423.7514953
CCTATTG1000.001651440423.7514953
GAGTACT45150.023.7171612-13
AAGGGTA1852.5383088E-723.1043474
GTATATG1903.264813E-722.5090431
ATAATGC1302.9363067E-421.9244583
ATGGGAG10400.021.9195065
TAATGCA1302.9407948E-421.9195064
GTACTTT48750.021.91703414-15
Produced by FastQC (version 0.11.5)