##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936974_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 442966 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.219409616087916 33.0 31.0 34.0 31.0 34.0 2 32.26325722515949 33.0 31.0 34.0 30.0 34.0 3 32.35966643038066 34.0 31.0 34.0 31.0 34.0 4 36.06797587173733 37.0 35.0 37.0 35.0 37.0 5 36.000144480614765 37.0 35.0 37.0 35.0 37.0 6 36.07211388684459 37.0 35.0 37.0 35.0 37.0 7 36.022548005941765 37.0 35.0 37.0 35.0 37.0 8 36.01128754802852 37.0 35.0 37.0 35.0 37.0 9 37.68128027884759 39.0 37.0 39.0 35.0 39.0 10-11 37.601161262941176 39.0 37.0 39.0 35.0 39.0 12-13 37.499071034797254 39.0 37.0 39.0 35.0 39.0 14-15 38.59529173796635 40.0 38.0 41.0 34.0 41.0 16-17 38.56565515186267 40.0 38.0 41.0 34.0 41.0 18-19 38.70226044436819 40.0 38.0 41.0 34.5 41.0 20-21 38.811829801835806 40.0 38.0 41.0 35.0 41.0 22-23 38.755639258995046 40.0 38.0 41.0 35.0 41.0 24-25 38.64417472221344 40.0 38.0 41.0 34.0 41.0 26-27 38.41225285913592 40.0 38.0 41.0 34.0 41.0 28-29 38.225958651454064 40.0 38.0 41.0 34.0 41.0 30-31 38.03451280685199 40.0 37.0 41.0 34.0 41.0 32-33 37.7907299883061 40.0 36.0 41.0 33.0 41.0 34-35 37.60311852376932 40.0 36.0 41.0 33.0 41.0 36-37 37.36300980210671 40.0 35.0 41.0 33.0 41.0 38-39 37.14878117056388 40.0 35.0 41.0 33.0 41.0 40-41 36.84835630725609 39.0 35.0 41.0 32.0 41.0 42-43 36.501593801781624 39.0 35.0 41.0 31.0 41.0 44-45 36.03097303179025 39.0 35.0 41.0 30.0 41.0 46-47 35.33622106437063 39.0 35.0 41.0 26.5 41.0 48-49 34.692193080281555 39.0 35.0 40.5 22.5 41.0 50-51 34.35237918937345 39.0 35.0 40.0 20.5 41.0 52-53 34.0507047944989 38.0 34.0 40.0 18.5 41.0 54-55 33.756523074005685 38.0 34.0 40.0 15.5 41.0 56-57 33.490515073391634 38.0 34.0 40.0 11.5 41.0 58-59 33.169478018628965 37.5 33.0 40.0 8.0 41.0 60-61 32.82721472979867 37.0 33.0 40.0 6.5 41.0 62-63 32.46463498327185 36.5 32.5 39.5 2.0 41.0 64-65 32.03295625397886 36.0 32.0 39.0 2.0 40.5 66-67 31.61156612471386 35.5 31.0 39.0 2.0 40.0 68-69 31.17546267659369 35.0 31.0 38.5 2.0 40.0 70-71 30.764346473544244 35.0 31.0 38.0 2.0 40.0 72-73 30.39982978377573 35.0 30.5 37.0 2.0 39.0 74-75 29.92272770370638 35.0 30.0 36.5 2.0 39.0 76-77 28.49115281985525 33.5 28.0 35.0 2.0 37.5 78-79 29.095961089564433 34.0 29.0 36.0 2.0 38.0 80-81 28.716600145383616 34.0 29.0 35.0 2.0 37.0 82-83 28.617925529273126 34.0 29.0 35.0 2.0 37.0 84-85 28.329086205261802 34.0 29.0 35.0 2.0 36.0 86-87 27.966894750387162 34.0 28.5 35.0 2.0 36.0 88-89 27.601248177060995 34.0 27.0 35.0 2.0 35.5 90-91 27.175901762212 33.5 26.0 35.0 2.0 35.0 92-93 26.80454030331899 33.0 25.5 35.0 2.0 35.0 94-95 26.369491112184683 33.0 25.0 35.0 2.0 35.0 96-97 25.778560882776556 33.0 23.5 35.0 2.0 35.0 98-99 25.10200670028851 32.5 19.0 34.5 2.0 35.0 100-101 22.336215420596616 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14325820709716908 1101 2 0.051356775900821106 1101 3 0.09718285874694033 1101 4 0.11041373843360702 1101 5 0.1965585788077533 1101 6 0.13061650617889597 1101 7 0.10784424180785379 1101 8 0.0677313986965018 1101 9 0.06913160652428019 1101 10-11 0.031514833188602154 1101 12-13 0.22053909669580918 1101 14-15 0.16722852418413225 1101 16-17 0.16494729344536552 1101 18-19 0.06043938942821114 1101 20-21 0.03770285556709041 1101 22-23 0.08427225504401292 1101 24-25 0.07723872041398039 1101 26-27 0.15521677204229434 1101 28-29 0.27962550902400807 1101 30-31 0.3398831079592881 1101 32-33 0.3644997148069393 1101 34-35 0.31150962167807705 1101 36-37 0.3810234869891431 1101 38-39 0.2827625196276884 1101 40-41 0.39856937594647235 1101 42-43 0.6030130046882789 1101 44-45 0.7502107998725052 1101 46-47 0.8707091991165115 1101 48-49 0.9593807193500936 1101 50-51 0.6838302077085672 1101 52-53 0.6675908778229669 1101 54-55 0.7846683795514622 1101 56-57 0.5743264577289011 1101 58-59 0.623538929918162 1101 60-61 0.6231707389142329 1101 62-63 0.6208731095180369 1101 64-65 0.5715505151597249 1101 66-67 0.5161812531593064 1101 68-69 0.6002890381359229 1101 70-71 0.6453913341849038 1101 72-73 0.8568153874407685 1101 74-75 0.7940660753137969 1101 76-77 0.5643854801478589 1101 78-79 0.8119229789749447 1101 80-81 1.1502014783531713 1101 82-83 0.9773675186388147 1101 84-85 0.729241387749962 1101 86-87 0.7103382593390606 1101 88-89 0.8458710112004795 1101 90-91 0.8802901392880038 1101 92-93 0.8866112316587156 1101 94-95 0.7886657810245836 1101 96-97 0.5192925139099529 1101 98-99 0.6384794477749871 1101 100-101 0.6790567601596678 1102 1 0.21578081592198117 1102 2 0.0763572602603162 1102 3 0.11176207950938277 1102 4 0.1044173679007443 1102 5 0.14258370953099586 1102 6 0.20664480138416508 1102 7 0.1182202985188141 1102 8 0.16028281076587803 1102 9 0.17891330031866914 1102 10-11 0.17757666759793267 1102 12-13 0.19632596560229842 1102 14-15 0.21113140687656795 1102 16-17 0.3248677293258524 1102 18-19 0.2030461261153249 1102 20-21 0.20233018937258151 1102 22-23 0.17981808513930986 1102 24-25 0.11591590234586846 1102 26-27 0.216251988206551 1102 28-29 0.27209802431362107 1102 30-31 0.21432528727563493 1102 32-33 0.32425266993802637 1102 34-35 0.2926901026362927 1102 36-37 0.46086422958759243 1102 38-39 0.4098756724635564 1102 40-41 0.5858475123894564 1102 42-43 0.3155860084275304 1102 44-45 0.3961472389906646 1102 46-47 0.5202164440581853 1102 48-49 0.6962615733727517 1102 50-51 0.4785005311100221 1102 52-53 0.6152789761414894 1102 54-55 0.4802043071859998 1102 56-57 0.5176628550845095 1102 58-59 0.563575224589556 1102 60-61 0.6918663695610618 1102 62-63 0.7394448517699033 1102 64-65 0.7861562142411636 1102 66-67 0.7404235534578909 1102 68-69 0.8456757830463779 1102 70-71 0.7346281127852556 1102 72-73 0.8519475745283884 1102 74-75 0.6152588482396091 1102 76-77 0.6657673039658576 1102 78-79 0.9455545342425182 1102 80-81 0.7962175676992018 1102 82-83 1.0372305848281513 1102 84-85 1.0077776964139709 1102 86-87 0.9076921496068806 1102 88-89 0.6911719706628219 1102 90-91 0.710530209380206 1102 92-93 0.915468293943043 1102 94-95 1.130591292992566 1102 96-97 1.0819562543277073 1102 98-99 1.0088589272930406 1102 100-101 1.177335669425144 1103 1 0.1393546346724861 1103 2 0.03858832404606005 1103 3 -0.004574753396568099 1103 4 0.1273150370243954 1103 5 0.05580542451323112 1103 6 0.16264045470799005 1103 7 0.11552374941172161 1103 8 0.10989068997888296 1103 9 0.02973778889426626 1103 10-11 0.17584892131502983 1103 12-13 0.06255786882551462 1103 14-15 0.3110733362028313 1103 16-17 0.08277195296119544 1103 18-19 0.14778699292074293 1103 20-21 0.1962974513115725 1103 22-23 0.2604931998432676 1103 24-25 0.12791957057257974 1103 26-27 0.12823035831797824 1103 28-29 0.08245846496079423 1103 30-31 0.1326138122805176 1103 32-33 0.011930187962938987 1103 34-35 0.36824378638685573 1103 36-37 0.2823055322105432 1103 38-39 0.43502429889088745 1103 40-41 0.4737093041859666 1103 42-43 0.28014277394849785 1103 44-45 0.5198090261875237 1103 46-47 0.7476249396252612 1103 48-49 0.6935578373010856 1103 50-51 0.3540309290953729 1103 52-53 0.5211915481548743 1103 54-55 0.1650515985627976 1103 56-57 0.21537625043656305 1103 58-59 0.4596373429617344 1103 60-61 0.5972586053394977 1103 62-63 0.4593257206451753 1103 64-65 0.5603101647718454 1103 66-67 0.6161336255575129 1103 68-69 0.5892348253977211 1103 70-71 0.5841201381293679 1103 72-73 0.5553846684648924 1103 74-75 0.7892164508982127 1103 76-77 0.7524305975504859 1103 78-79 0.8875025466155968 1103 80-81 1.162784383471429 1103 82-83 1.0497833371481065 1103 84-85 0.9081563057075783 1103 86-87 0.6788189506602009 1103 88-89 0.7345122418126238 1103 90-91 0.5377052723380125 1103 92-93 0.6374721921526252 1103 94-95 0.6974760600774204 1103 96-97 1.0372964564826468 1103 98-99 0.693952012858162 1103 100-101 1.1968166555916717 1104 1 0.16669448416226373 1104 2 0.061789954167586814 1104 3 0.01307001324764201 1104 4 0.017133677886214116 1104 5 0.09440309785571088 1104 6 0.09770146249328349 1104 7 0.13708145717509268 1104 8 0.10308566322962776 1104 9 0.16125360708051772 1104 10-11 0.18612648035416868 1104 12-13 0.17807385749259197 1104 14-15 0.11467129368003981 1104 16-17 0.1719100911480851 1104 18-19 0.06446448376755143 1104 20-21 0.0411101659347608 1104 22-23 0.05998946956018614 1104 24-25 0.12580581114539768 1104 26-27 0.3316028345927293 1104 28-29 0.07469448350261132 1104 30-31 0.2511030679287103 1104 32-33 0.1579957337857465 1104 34-35 -0.2736723776319465 1104 36-37 -0.3935089196935664 1104 38-39 -0.3990247833539371 1104 40-41 -0.46782287204250395 1104 42-43 -0.2785229293661331 1104 44-45 -0.0583726739123307 1104 46-47 -0.19349930031433615 1104 48-49 -0.1760781049302622 1104 50-51 -0.04005379176255275 1104 52-53 -0.06121259696491421 1104 54-55 -0.13448018799519446 1104 56-57 -0.3421427662949128 1104 58-59 -0.07258962385944301 1104 60-61 -0.11782428081989593 1104 62-63 -0.17650894541534257 1104 64-65 -0.07576398627417547 1104 66-67 -0.321211622492104 1104 68-69 -0.058126577740747365 1104 70-71 -0.09688484640558315 1104 72-73 -0.14929178326061887 1104 74-75 0.041596079249988094 1104 76-77 -0.008916525788340124 1104 78-79 0.30579453158081193 1104 80-81 0.37214094008324494 1104 82-83 0.4054621756053365 1104 84-85 0.3663347179627152 1104 86-87 0.19459654623094735 1104 88-89 -0.04845753437039235 1104 90-91 -0.04809631232195599 1104 92-93 0.2897247716900573 1104 94-95 0.31062256806160704 1104 96-97 0.4519586202903767 1104 98-99 0.428574235441193 1104 100-101 0.5666474330654729 1105 1 -0.003218635073267251 1105 2 -0.17310709572797123 1105 3 -0.029684377952463592 1105 4 0.11114874910923334 1105 5 0.01967466455633371 1105 6 0.0778934713837316 1105 7 0.25532573172242934 1105 8 0.10774354235114458 1105 9 0.030273908985165576 1105 10-11 -0.11864904622969874 1105 12-13 0.06332470726398753 1105 14-15 -0.02890148482374144 1105 16-17 0.10673065304334983 1105 18-19 0.03493771468241391 1105 20-21 0.10029061244632942 1105 22-23 -0.23853324874788484 1105 24-25 0.1828665453616054 1105 26-27 0.08390176204574828 1105 28-29 -0.10389127738149284 1105 30-31 0.006677602569546082 1105 32-33 0.04085933475848691 1105 34-35 0.07979650752972844 1105 36-37 0.07370413883175786 1105 38-39 0.17511541263370134 1105 40-41 0.4925628607159709 1105 42-43 0.3785381478455889 1105 44-45 0.5106122994540669 1105 46-47 0.6923792312532484 1105 48-49 0.6784917958411398 1105 50-51 0.7469322723295875 1105 52-53 0.7040258363687641 1105 54-55 0.6432587169573196 1105 56-57 0.6623110304939601 1105 58-59 0.7147531555704276 1105 60-61 0.7528921339165677 1105 62-63 0.7568830401514077 1105 64-65 0.46273897549211895 1105 66-67 0.7607780259031962 1105 68-69 0.8665756164015725 1105 70-71 0.8853942902061007 1105 72-73 0.9490861909392585 1105 74-75 0.9107570093166295 1105 76-77 0.8605668200643315 1105 78-79 0.7537672565772944 1105 80-81 1.2344350998985725 1105 82-83 1.1542694086948018 1105 84-85 0.7136313987950977 1105 86-87 0.6862926247632011 1105 88-89 0.8279251663077076 1105 90-91 0.7315393578398321 1105 92-93 0.6570352409422213 1105 94-95 0.6629509931290904 1105 96-97 0.8407937732149264 1105 98-99 0.9458217650159781 1105 100-101 0.6253939902479679 1106 1 0.13840564512040743 1106 2 -0.018652315603020497 1106 3 0.058540583479661734 1106 4 0.06996895409174186 1106 5 0.16006171797092605 1106 6 0.06006824112207454 1106 7 0.04391269654546903 1106 8 0.1429968683527605 1106 9 0.2256182704386802 1106 10-11 0.22772099149808156 1106 12-13 -0.11730276528027161 1106 14-15 -0.0019727093727723854 1106 16-17 -0.04776231309329404 1106 18-19 0.07009939425596201 1106 20-21 -0.03111509101638177 1106 22-23 -0.14689445786976307 1106 24-25 -0.13709404667432779 1106 26-27 -0.1559980378595469 1106 28-29 -0.14161905694128052 1106 30-31 -0.5201900752627111 1106 32-33 -0.4650289482456813 1106 34-35 -0.398101428898201 1106 36-37 -0.36209753829787417 1106 38-39 -0.6422867166183082 1106 40-41 -0.4771556320057826 1106 42-43 -0.5479003192151595 1106 44-45 -0.35440014918575 1106 46-47 -0.11973795397945253 1106 48-49 -0.32479983836741866 1106 50-51 -0.5525071570186526 1106 52-53 -0.5132296585983127 1106 54-55 -0.3771784365046287 1106 56-57 -0.5440232651268673 1106 58-59 -0.4463765393997079 1106 60-61 -0.6376132961342975 1106 62-63 -0.6870446202680256 1106 64-65 -0.7470931588004497 1106 66-67 -0.6625062765911167 1106 68-69 -0.5371268992928755 1106 70-71 0.019243898417620642 1106 72-73 -0.2340691955795542 1106 74-75 -0.15654201770379217 1106 76-77 -0.06451986218223027 1106 78-79 0.14760250684841125 1106 80-81 0.1251772832219764 1106 82-83 -0.03774513253845413 1106 84-85 -0.05821481849858401 1106 86-87 0.05881244330327107 1106 88-89 -0.1634066090363504 1106 90-91 -0.20866898504000275 1106 92-93 0.0618527881783173 1106 94-95 0.09688919288775466 1106 96-97 0.3116960322993805 1106 98-99 0.2826147510093513 1106 100-101 0.25804455769210577 1107 1 -0.025444227362555694 1107 2 0.03362966833363856 1107 3 -0.039019463720670444 1107 4 0.050841398992965026 1107 5 -0.10939890400768348 1107 6 -0.04636090127828396 1107 7 0.0043062864354652675 1107 8 0.14503333441800947 1107 9 0.01913583733754365 1107 10-11 0.0677156624280073 1107 12-13 0.19494267646157226 1107 14-15 -0.06357104974808436 1107 16-17 0.25875437831548 1107 18-19 0.10468125257882122 1107 20-21 0.13619867701681443 1107 22-23 0.01545185555569617 1107 24-25 -0.022252586509118544 1107 26-27 -0.21962332810354468 1107 28-29 -0.04667596379675132 1107 30-31 0.059393876623396125 1107 32-33 -0.1689610406669857 1107 34-35 -0.23506043312339386 1107 36-37 -0.1786633332809373 1107 38-39 0.09706465942371523 1107 40-41 0.35131492488203975 1107 42-43 -0.26491718191547875 1107 44-45 -0.05427834186968994 1107 46-47 -0.07330843093122752 1107 48-49 -0.33577201300866477 1107 50-51 -0.07168229310026675 1107 52-53 -0.288913873131456 1107 54-55 -0.3986564921553466 1107 56-57 -0.3858640781906999 1107 58-59 0.03178378948004479 1107 60-61 -0.5212206959287471 1107 62-63 -0.051781402270997035 1107 64-65 -0.1377901250369824 1107 66-67 -0.23375404028492497 1107 68-69 -0.4299650097568879 1107 70-71 -0.5106559911945006 1107 72-73 -0.5055948322131023 1107 74-75 -0.15552378467116768 1107 76-77 -0.13062887973009651 1107 78-79 -0.28222505966980194 1107 80-81 0.03978845143026888 1107 82-83 -0.35501512606744257 1107 84-85 -0.5455773094581993 1107 86-87 -0.29488555068249056 1107 88-89 -0.5855736373184826 1107 90-91 -0.43521156050344345 1107 92-93 -0.35133095054498753 1107 94-95 -0.17346595156810807 1107 96-97 -0.07471864548220708 1107 98-99 -0.06502142576351488 1107 100-101 0.3960103755140345 1108 1 -0.013052136023098626 1108 2 -0.06289432137921125 1108 3 -0.11781121433904929 1108 4 0.11484945835706384 1108 5 -0.13591945374804482 1108 6 -0.0560419666869052 1108 7 -0.07534545730296571 1108 8 0.011406784382785418 1108 9 -0.06284198303036703 1108 10-11 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-1.215660817119776 2316 54-55 -1.0318712339907776 2316 56-57 -1.2484803890828715 2316 58-59 -1.1832408410618243 2316 60-61 -0.7300055891964021 2316 62-63 -0.621841610262237 2316 64-65 -0.5177207946228286 2316 66-67 -0.7806159268019819 2316 68-69 -1.1316526579962591 2316 70-71 -0.8187202130518259 2316 72-73 -0.6748613894106938 2316 74-75 -0.5280379244266875 2316 76-77 -0.8126303393177636 2316 78-79 -0.5539273037858372 2316 80-81 -0.6821327308357432 2316 82-83 -0.2105889264688301 2316 84-85 -0.5888358389236252 2316 86-87 -0.8434533302847562 2316 88-89 -0.8718991253681097 2316 90-91 -0.7179884260389251 2316 92-93 -0.38808519247164597 2316 94-95 -0.767007276143918 2316 96-97 -1.3433192658069615 2316 98-99 -2.0278293075369156 2316 100-101 -2.5764216624352443 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 6.0 5 18.0 6 36.0 7 71.0 8 170.0 9 322.0 10 625.0 11 1058.0 12 1698.0 13 2337.0 14 2998.0 15 4362.0 16 6438.0 17 9521.0 18 8248.0 19 4270.0 20 3235.0 21 3060.0 22 3032.0 23 3152.0 24 3571.0 25 4115.0 26 4749.0 27 5695.0 28 6699.0 29 8108.0 30 10035.0 31 12544.0 32 15700.0 33 20458.0 34 28621.0 35 41851.0 36 61289.0 37 90028.0 38 68900.0 39 5943.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.12264990267673 19.568525970184307 15.51549720675437 18.793326920384594 2 15.655401454415738 22.40030524217309 40.57688964547205 21.367403657939118 3 19.531105361311678 28.735826819653475 31.133131939834826 20.599935879200025 4 12.355575822975126 17.91401597413797 38.052356162775475 31.67805204011143 5 12.118632971061935 39.96333638863428 32.4633251005766 15.45470553972719 6 28.13827697836854 38.28668565984748 17.617379211948546 15.957658149835426 7 25.974905523223 36.913894068619264 20.19658393646465 16.914616471693087 8 27.12945718058989 32.8314878150644 19.42659126567562 20.612463738670098 9 28.77083116988663 16.586148824063248 20.609708194308364 34.033311811741754 10-11 24.638459836646607 28.12574780005689 25.855031763160152 21.38076060013635 12-13 27.74185829160703 26.47302501772145 26.966629493008494 18.818487197663025 14-15 24.31541021207045 25.933931723879482 28.63413896326129 21.116519100788775 16-17 20.12276337235815 29.604868093713737 28.808418704821587 21.463949829106525 18-19 19.8698259718182 26.527304149156052 34.499729650713235 19.103140228312512 20-21 21.849930863222056 24.95346671557976 34.42221407003979 18.774388351158393 22-23 20.945061246235603 25.67725288171101 34.088169295160355 19.289516576893035 24-25 20.648407145775906 25.575746082488426 34.82030581536843 18.95554095636724 26-27 20.75926847797498 25.50686944831066 34.86221030185838 18.871651771855976 28-29 19.970311671012677 25.69124137853184 35.01427974759277 19.32416720286272 30-31 20.013278128235555 26.24304620133752 34.80868091506176 18.934994755365164 32-33 19.78874129726118 26.247279688642873 34.369948799450974 19.594030214644985 34-35 20.321660860907865 25.845296658095364 34.34206040848762 19.49098207250915 36-37 20.747423844229502 25.830420764583163 34.20416881158164 19.217986579605697 38-39 20.855016153465826 25.81506708598701 34.24354143572817 19.086375324818995 40-41 20.296886641229992 26.200076761565033 34.23689973584991 19.26613686135507 42-43 20.028492472749278 26.84975300503021 33.57968861475106 19.542065907469453 44-45 20.435279270899883 27.166406671527938 32.619644796221756 19.778669261350423 46-47 20.790524630947786 27.242701920493772 31.5467119981759 20.42006145038254 48-49 21.199615765359507 27.561385547404555 30.863162124668563 20.375836562567372 50-51 21.516585430828382 28.03674915170821 30.342996597802458 20.103668819660957 52-53 21.174796000031606 28.381308647521692 30.403755698961625 20.040139653485074 54-55 20.550188844515382 28.273286120323192 30.445844630517183 20.730680404644243 56-57 21.119573057373803 28.865696609314313 29.519249328669297 20.495481004642592 58-59 21.1465743188483 28.116795307960256 29.965482444357654 20.771147928833784 60-61 21.203546096088637 27.847961243075087 30.367680589480912 20.580812071355364 62-63 21.736882740436062 27.76048274585408 30.30954971713405 20.193084796575807 64-65 21.525370347584317 27.927193666697896 30.344626728693232 20.202809257024555 66-67 21.077428763641127 28.1577200753112 30.116353095750874 20.648498065296796 68-69 21.200279481266517 28.549191976314077 29.941857511962045 20.30867103045736 70-71 21.184297860235784 28.543208427212345 29.606317700125974 20.666176012425893 72-73 21.146417290746164 28.294564017348545 29.1400910776011 21.418927614304195 74-75 21.306144103669755 28.573750691395094 29.393604172075538 20.72650103285961 76-77 21.31244137258421 28.322333108700732 29.548443318647553 20.8167822000675 78-79 21.004772914051422 28.20997660956028 29.511352039664406 21.273898436723893 80-81 21.089001287910683 29.116037253465564 29.051811032687645 20.743150425936104 82-83 20.946434840324958 29.025740128886895 28.822668200335706 21.205156830452438 84-85 20.9937161865481 28.464422012197392 28.68080552912373 21.861056272130774 86-87 21.079044441334098 28.179363653192347 28.793180514983092 21.948411390490467 88-89 21.372399181418704 27.800384457791477 28.966964397583084 21.860251963206736 90-91 20.832035253588344 28.005788306897657 29.441125875357255 21.721050564156744 92-93 20.428001729254312 28.389456966738948 29.20001670558994 21.982524598416806 94-95 20.22715292412546 28.787039054184167 28.778460430534032 22.207347591156342 96-97 20.367477413616395 28.558963893391365 28.49609225087253 22.57746644211971 98-99 20.362782612621764 28.827108236542394 28.211483977289404 22.598625173546445 100-101 20.49439851003189 29.320089172334 28.164235121483195 22.021277196150916 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1005.0 1 702.5 2 460.5 3 519.5 4 436.0 5 420.0 6 725.5 7 1603.0 8 2596.5 9 2824.0 10 2266.0 11 1712.0 12 1635.0 13 1681.0 14 1722.0 15 1743.5 16 1784.0 17 1837.5 18 1894.0 19 1969.0 20 1976.0 21 2130.0 22 2419.0 23 2733.0 24 3039.5 25 3380.5 26 3885.5 27 4530.5 28 5097.0 29 5629.5 30 6239.0 31 6948.5 32 7864.0 33 8632.0 34 9403.0 35 10061.5 36 10811.0 37 11754.0 38 12036.0 39 11879.5 40 11767.0 41 12100.0 42 12559.5 43 12943.0 44 13576.0 45 14261.0 46 14496.5 47 15077.5 48 20627.0 49 20545.0 50 14718.5 51 12946.0 52 11682.5 53 11288.5 54 11063.5 55 10498.0 56 9572.0 57 8630.0 58 8019.0 59 7485.5 60 6734.0 61 5785.5 62 4823.0 63 3566.5 64 2375.5 65 1686.5 66 1206.5 67 962.5 68 725.5 69 453.0 70 321.5 71 227.5 72 177.0 73 167.0 74 131.5 75 96.5 76 65.0 77 41.0 78 27.0 79 17.5 80 14.0 81 9.5 82 7.0 83 3.0 84 2.0 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025284107583877773 2 0.008804287462243153 3 0.011739049949657535 4 0.0 5 0.004515019211406745 6 0.0 7 0.0 8 2.2575096057033723E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0046278946916919125 20-21 7.901283619961804E-4 22-23 0.0 24-25 0.004289268250836407 26-27 0.005982400455113937 28-29 0.006998279777680454 30-31 0.029912002275569685 32-33 4.5150192114067446E-4 34-35 0.003386264408555058 36-37 0.013093555713079559 38-39 0.006095275935399105 40-41 0.00812703458053214 42-43 0.009481540343954164 44-45 0.002031758645133035 46-47 0.0015802567239923608 48-49 0.001693132204277529 50-51 0.003837766329695733 52-53 0.0028218870071292155 54-55 0.002031758645133035 56-57 5.643774014258431E-4 58-59 6.772528817110116E-4 60-61 0.0 62-63 0.0 64-65 0.008465661021387646 66-67 9.030038422813489E-4 68-69 5.643774014258431E-4 70-71 0.004515019211406745 72-73 0.01061029514680585 74-75 0.004740770171977082 76-77 0.0046278946916919125 78-79 0.011287548028516862 80-81 1.1287548028516862E-4 82-83 0.004402143731121576 84-85 5.643774014258431E-4 86-87 0.0 88-89 0.0010158793225665175 90-91 9.030038422813489E-4 92-93 1.1287548028516862E-4 94-95 0.0010158793225665175 96-97 0.0 98-99 2.2575096057033723E-4 100-101 7.901283619961804E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 442966.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.769064372787476 #Duplication Level Percentage of deduplicated Percentage of total 1 82.22636989242783 49.9681956513358 2 10.712505910470322 13.019779225344744 3 3.1011557424965606 5.65362998837439 4 1.3253758030699319 3.221673899795663 5 0.6819381036161953 2.0720370258454595 6 0.41418223778773827 1.5101680242112943 7 0.2577602624835243 1.0964694988525516 8 0.20955256360949553 1.018745058197446 9 0.14746969975082888 0.8065436109474385 >10 0.8331182003634187 9.260824890626298 >50 0.05605044743766108 2.3567735216132197 >100 0.027839626198760486 3.1460490328368445 >500 0.0029695601278677854 1.0750749043163692 >1k 0.0029695601278677854 3.063940920127831 >5k 7.423900319669463E-4 2.730094747574699 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6914 1.5608421413833116 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5189 1.17142173439948 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3976 0.8975858192276608 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2331 0.526225489089456 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1732 0.39100066370782405 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1246 0.2812856968706402 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1162 0.2623226161827319 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1074 0.2424565316525422 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1035 0.23365224419029906 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1027 0.23184623650573633 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 727 0.16412094833463517 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 724 0.16344369545292414 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 641 0.14470636572558618 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 595 0.13432182153935066 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 540 0.1219055187079821 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 517 0.11671324661486435 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 511 0.11535874085144232 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 511 0.11535874085144232 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 486 0.10971496683718389 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 473 0.10678020434976951 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 2.2575096057033723E-4 0.0 0.0 0.0 0.0 9 2.2575096057033723E-4 0.0 0.0 0.0 0.0 10-11 2.2575096057033723E-4 0.0 0.0 0.0 0.0 12-13 2.2575096057033723E-4 0.0 0.0 0.0 0.0 14-15 2.2575096057033723E-4 0.0 0.0 0.0 0.0 16-17 2.2575096057033723E-4 0.0 0.0 0.0 0.0 18-19 3.386264408555058E-4 0.0 0.0 0.0 0.0 20-21 4.5150192114067446E-4 0.0 0.0 2.2575096057033723E-4 0.0 22-23 4.5150192114067446E-4 0.0 0.0 6.772528817110116E-4 0.0 24-25 4.5150192114067446E-4 0.0 0.0 0.001693132204277529 0.0 26-27 4.5150192114067446E-4 0.0 0.0 0.002596136046558878 0.0 28-29 4.5150192114067446E-4 0.0 0.0 0.005192272093117756 0.0 30-31 4.5150192114067446E-4 0.0 0.0 0.010610295146805851 0.0 32-33 4.5150192114067446E-4 0.0 0.0 0.016366944641349447 0.0 34-35 4.5150192114067446E-4 0.0 0.0 0.02201071865560788 0.0 36-37 4.5150192114067446E-4 0.0 0.0 0.027880243630436646 0.0 38-39 6.772528817110116E-4 0.0 0.0 0.03645878013210946 0.0 40-41 6.772528817110116E-4 0.0 0.0 0.04356993539007509 0.0 42-43 6.772528817110116E-4 0.0 0.0 0.06264589155826858 0.0 44-45 6.772528817110116E-4 0.0 0.0 0.08386648185188028 0.0 46-47 6.772528817110116E-4 0.0 0.0 0.09978192457208906 0.0 48-49 6.772528817110116E-4 0.0 0.0 0.1229213980305486 0.0 50-51 6.772528817110116E-4 0.0 0.0 0.15813854787952125 0.0 52-53 6.772528817110116E-4 0.0 0.0 0.22857284757746643 0.0 54-55 6.772528817110116E-4 0.0 0.0 0.27710930410008894 0.0 56-57 0.0011287548028516862 0.0 0.0 0.3645878013210946 0.0 58-59 0.0011287548028516862 0.0 0.0 0.47193238307228996 0.0 60-61 0.0011287548028516862 0.0 0.0 0.5876297503645878 0.0 62-63 0.0011287548028516862 0.0 0.0 0.7563785933909148 0.0 64-65 0.0011287548028516862 0.0 0.0 0.8281673988522822 0.0 66-67 0.0015802567239923606 0.0 0.0 1.0219745985019166 0.0 68-69 0.0015802567239923606 0.0 0.0 1.2640925037136033 0.0 70-71 0.0015802567239923606 0.0 0.0 1.409814748761756 0.0 72-73 0.0016931322042775293 0.0 0.0 1.7151429229331372 0.0 74-75 0.0018060076845626979 0.0 0.0 2.0041041524631686 0.0 76-77 0.0018060076845626979 0.0 0.0 2.149036269149325 0.0 78-79 0.0018060076845626979 0.0 0.0 2.3050301829034283 0.0 80-81 0.0018060076845626979 0.0 0.0 2.624242041149885 0.0 82-83 0.002031758645133035 0.0 0.0 2.8777603698703738 0.0 84-85 0.0022575096057033724 0.0 0.0 3.337276450111295 0.0 86-87 0.0024832605662737095 0.0 0.0 3.9868748391524407 0.0 88-89 0.0024832605662737095 0.0 0.0 4.826216910552954 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCGA 20 0.0020708577 71.270584 2 CTAAGGG 135 1.1903467E-8 31.675817 2 ATATGGG 130 3.0280717E-7 29.23261 3 GGTAGTT 135 4.2254032E-7 28.14039 7 TAAGGGT 165 8.38063E-8 25.910723 3 AGGGTAG 205 9.549694E-10 25.483654 5 GTATCAA 8135 0.0 25.000824 1 GTAAGGT 135 1.3419971E-5 24.625622 4 AGTACTT 4540 0.0 24.266436 12-13 GTGTAGG 240 2.2737368E-10 23.759544 1 GTATAGG 120 1.703854E-4 23.759544 1 CATATAG 100 0.0016514404 23.751495 3 CCTATTG 100 0.0016514404 23.751495 3 GAGTACT 4515 0.0 23.71716 12-13 AAGGGTA 185 2.5383088E-7 23.104347 4 GTATATG 190 3.264813E-7 22.509043 1 ATAATGC 130 2.9363067E-4 21.924458 3 ATGGGAG 1040 0.0 21.919506 5 TAATGCA 130 2.9407948E-4 21.919506 4 GTACTTT 4875 0.0 21.917034 14-15 >>END_MODULE