FastQCFastQC Report
Thu 26 May 2016
SRR936973_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936973_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492828
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74101.5035671674499014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50141.0173934922528751No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36000.730477976089021No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17780.3607749559684109No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT15030.3049745550171662No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14150.28711842671276794No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10940.2219841405114969No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10850.22015794557127435No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT9250.18769225774509565No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA9020.18302531512008247No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8970.18201076237551436No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG8780.17815546194615567No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7430.15076253784281737No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC7190.14589268466889058No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG5180.10510766433725355No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4970.10084654281006761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC1509.440555E-1031.690033
GTATCAA69250.031.2355841
GGTATCA45850.029.8614671
GTACATG45400.026.3877681
GATTCGT458.955852E-426.38423566-67
TACATGG45600.025.9539972
TATCAAC83400.025.8167722
ACATGGG44650.025.444293
ATCAACG85000.025.2774583
GAGTACT44400.025.1516512-13
TCAACGC85900.025.0126194
CAGACGC950.001222470525.0108229
CAACGCA86150.024.9400335
AACGCAG86950.024.8695246
ATGGGAG10650.024.5486155
CATGGGA33300.024.1243924
AGTACTT44700.024.02605612-13
TATGGGC1000.001645229923.7675234
GTACTTT47950.023.23762314-15
ACGCAGA93300.023.0750277
Produced by FastQC (version 0.11.5)