##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936973_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 492828 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.888104977801586 33.0 31.0 34.0 30.0 34.0 2 31.96207196019707 33.0 31.0 34.0 30.0 34.0 3 31.922421209833857 33.0 31.0 34.0 30.0 34.0 4 35.53344777488292 37.0 35.0 37.0 33.0 37.0 5 35.54031426785816 37.0 35.0 37.0 33.0 37.0 6 35.664225652763236 37.0 35.0 37.0 33.0 37.0 7 35.624475476231055 37.0 35.0 37.0 33.0 37.0 8 35.578144910597615 37.0 35.0 37.0 33.0 37.0 9 37.18340475784655 39.0 37.0 39.0 34.0 39.0 10-11 37.069581070880716 39.0 37.0 39.0 33.0 39.0 12-13 36.96982009950733 39.0 37.0 39.0 33.0 39.0 14-15 38.02788396763171 40.0 38.0 41.0 33.0 41.0 16-17 38.00465578254482 40.0 38.0 41.0 33.0 41.0 18-19 38.08742299544669 40.0 38.0 41.0 33.0 41.0 20-21 38.04934074362658 40.0 38.0 41.0 33.0 41.0 22-23 37.91532339883286 40.0 38.0 41.0 33.0 41.0 24-25 37.97897440892157 40.0 38.0 41.0 33.0 41.0 26-27 37.88576440461986 40.0 37.5 41.0 33.0 41.0 28-29 37.7571252039251 40.0 37.0 41.0 33.0 41.0 30-31 37.45059838320874 39.5 37.0 41.0 32.5 41.0 32-33 37.115086805132826 39.0 35.5 41.0 32.0 41.0 34-35 36.85407890785426 39.0 35.0 41.0 31.0 41.0 36-37 36.59429963394937 39.0 35.0 41.0 31.0 41.0 38-39 36.37368006687932 39.0 35.0 41.0 30.0 41.0 40-41 36.088226521220385 39.0 35.0 40.5 30.0 41.0 42-43 35.74469388914591 39.0 35.0 40.0 29.5 41.0 44-45 35.27354776920143 38.0 35.0 40.0 26.5 41.0 46-47 34.622724358193935 38.0 34.5 40.0 23.5 41.0 48-49 34.10291623040899 38.0 34.0 40.0 19.0 41.0 50-51 33.27941086951228 37.5 33.5 39.5 14.5 40.5 52-53 33.403689522510895 38.0 33.5 39.5 13.5 40.5 54-55 33.75799366107445 38.0 34.0 40.0 11.0 41.0 56-57 33.654055776051685 38.0 34.0 40.0 8.5 41.0 58-59 33.40362560568799 38.0 34.0 40.0 7.0 41.0 60-61 33.126242827112094 38.0 33.0 40.0 4.0 41.0 62-63 32.778229930117604 37.0 33.0 40.0 2.0 41.0 64-65 32.411184023635016 36.5 32.5 40.0 2.0 41.0 66-67 32.03586139586225 36.0 32.0 39.0 2.0 41.0 68-69 31.64226565860706 35.5 32.0 39.0 2.0 41.0 70-71 31.2316771774331 35.0 31.0 39.0 2.0 40.0 72-73 30.815116024251868 35.0 31.0 38.0 2.0 40.0 74-75 30.378720364914333 35.0 30.5 37.0 2.0 39.5 76-77 29.93403276599544 35.0 30.0 37.0 2.0 39.0 78-79 29.51391661999724 35.0 29.5 36.0 2.0 39.0 80-81 29.073032579317733 34.0 29.0 36.0 2.0 37.5 82-83 28.66979250367268 34.0 29.0 35.0 2.0 37.0 84-85 28.298673167920654 34.0 29.0 35.0 2.0 37.0 86-87 27.936159268548053 34.0 27.5 35.0 2.0 36.0 88-89 27.566836705706656 34.0 26.5 35.0 2.0 36.0 90-91 27.146248792682233 34.0 26.0 35.0 2.0 35.5 92-93 26.81947352828979 34.0 25.0 35.0 2.0 35.0 94-95 26.450215896824044 34.0 25.0 35.0 2.0 35.0 96-97 25.958848726127574 33.0 23.5 35.0 2.0 35.0 98-99 25.36498940806935 33.0 19.0 35.0 2.0 35.0 100-101 23.095271575478666 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4029939856046312 1101 2 0.2886322278270548 1101 3 0.28763367462515177 1101 4 0.19830637746309776 1101 5 0.2630474785294439 1101 6 -0.011593959614572213 1101 7 -0.003547534098075289 1101 8 -0.04083480291664188 1101 9 0.2525754759177161 1101 10-11 0.3325095003267293 1101 12-13 0.22736948478300434 1101 14-15 0.4272513581750701 1101 16-17 0.4696809363286931 1101 18-19 0.4976394803652582 1101 20-21 0.3106675340904985 1101 22-23 0.41653451390592267 1101 24-25 0.437015541167149 1101 26-27 0.4794582929377782 1101 28-29 0.3990307851898436 1101 30-31 0.5059712837337926 1101 32-33 0.6884351180493482 1101 34-35 0.8597137092977292 1101 36-37 0.6831659495642199 1101 38-39 0.8324002471511847 1101 40-41 0.8178832647378229 1101 42-43 0.8319649696000155 1101 44-45 0.9790847131040579 1101 46-47 1.2500969791698253 1101 48-49 1.3666733436601177 1101 50-51 1.6131769445459625 1101 52-53 1.779542766198098 1101 54-55 1.7673723537413082 1101 56-57 1.9254065636763045 1101 58-59 1.8867296140111804 1101 60-61 1.9431327718608955 1101 62-63 1.8259442151036254 1101 64-65 1.7565882597845714 1101 66-67 1.823872758930495 1101 68-69 1.7533996148093074 1101 70-71 1.7218805729269242 1101 72-73 1.594720257163516 1101 74-75 1.7874423238937496 1101 76-77 1.9375332642700407 1101 78-79 1.891789195318136 1101 80-81 1.9037055608419244 1101 82-83 1.7578495843556645 1101 84-85 1.7257254272621658 1101 86-87 1.7754923069541917 1101 88-89 1.9274439857794157 1101 90-91 1.7231809860265521 1101 92-93 1.8007633309432691 1101 94-95 1.9320699073970076 1101 96-97 1.7735603585393207 1101 98-99 1.5306716686734951 1101 100-101 2.0148612225920353 1102 1 0.15582440280373433 1102 2 0.1841174271586361 1102 3 0.09933589191694026 1102 4 0.11709490624295427 1102 5 0.2748694071098967 1102 6 0.13409397657685673 1102 7 0.07557425546281848 1102 8 0.07253986182153938 1102 9 0.1973722475745774 1102 10-11 0.21038248348991573 1102 12-13 0.11285036608277466 1102 14-15 0.1152696097583501 1102 16-17 0.2894601550106728 1102 18-19 0.3025443081354595 1102 20-21 0.27559844558138025 1102 22-23 0.2768646151514744 1102 24-25 -0.06290722574339469 1102 26-27 0.051183697709376474 1102 28-29 0.11049191237167832 1102 30-31 -0.1419270108191668 1102 32-33 0.22906542782424566 1102 34-35 0.5037503493774906 1102 36-37 0.3673142090112336 1102 38-39 0.2663865804049479 1102 40-41 0.4175490560961421 1102 42-43 0.5583610454569552 1102 44-45 0.42629194703607 1102 46-47 0.7074888879505323 1102 48-49 0.8219277389250337 1102 50-51 0.7078879699205416 1102 52-53 0.9428446738554044 1102 54-55 0.8521431294423749 1102 56-57 1.0115271631455016 1102 58-59 1.102618040281662 1102 60-61 0.8454925938035203 1102 62-63 1.074453528712091 1102 64-65 1.084240865348164 1102 66-67 0.9256453460985732 1102 68-69 1.0557267882186459 1102 70-71 0.9604237462239311 1102 72-73 0.9306855128050451 1102 74-75 0.8971291086100237 1102 76-77 1.057334511935494 1102 78-79 0.862503812031381 1102 80-81 1.037195495966131 1102 82-83 1.0126753359740448 1102 84-85 1.009406514282798 1102 86-87 0.9763812773668974 1102 88-89 0.8230283594190588 1102 90-91 0.8850514485825478 1102 92-93 0.697107309570768 1102 94-95 0.8595339940104054 1102 96-97 0.8176371949315566 1102 98-99 0.7738422449727658 1102 100-101 0.5840765519560556 1103 1 0.04750989055816035 1103 2 0.1980111202290864 1103 3 0.2541390561204757 1103 4 0.263668790033762 1103 5 0.3883588706919454 1103 6 0.2659003714511883 1103 7 0.2222996122722094 1103 8 0.25173809478703646 1103 9 0.23058440249304368 1103 10-11 0.2545866058168045 1103 12-13 0.4438748662783283 1103 14-15 0.4603477540103995 1103 16-17 0.47379490586017425 1103 18-19 0.2993006377859686 1103 20-21 0.3258818357736146 1103 22-23 0.3349437790204135 1103 24-25 0.20525992812380878 1103 26-27 0.28677341382181254 1103 28-29 0.28875304733414 1103 30-31 0.5214021626581342 1103 32-33 0.7044109332580604 1103 34-35 0.5017913736968254 1103 36-37 0.5643099564627505 1103 38-39 0.7625336267941236 1103 40-41 0.7963972055254018 1103 42-43 0.7752766252481891 1103 44-45 0.9999609626649146 1103 46-47 0.9976936704284896 1103 48-49 1.1775016068902957 1103 50-51 1.0796174526740998 1103 52-53 1.0173236179754284 1103 54-55 1.2864580983677953 1103 56-57 1.2853988587163911 1103 58-59 1.3275311693751064 1103 60-61 1.4913825882193592 1103 62-63 1.498959489288417 1103 64-65 1.6208572067999967 1103 66-67 1.4537023316046422 1103 68-69 1.4506186432657522 1103 70-71 1.5794346274125708 1103 72-73 1.2738515927963654 1103 74-75 1.447933046004188 1103 76-77 1.4501396565922242 1103 78-79 1.3542864007730913 1103 80-81 1.557202933969581 1103 82-83 1.5829260351237657 1103 84-85 1.5171355946154925 1103 86-87 1.6003851161905622 1103 88-89 1.5771836938692196 1103 90-91 1.216684666841605 1103 92-93 1.482577429822257 1103 94-95 1.4277104171220252 1103 96-97 1.1943240278568155 1103 98-99 1.2127392727104116 1103 100-101 1.3377938580875472 1104 1 0.48155114131100163 1104 2 0.4172236238081659 1104 3 0.4395633951836295 1104 4 0.3977189267793122 1104 5 0.3999600278456583 1104 6 0.35179500990647483 1104 7 0.2637412494011784 1104 8 0.28523993287108595 1104 9 0.3738052128297582 1104 10-11 0.4115872870762516 1104 12-13 0.47871865381753054 1104 14-15 0.7228120043452719 1104 16-17 0.5017709729197932 1104 18-19 0.43197097732961964 1104 20-21 0.512999450114954 1104 22-23 0.5019831637147902 1104 24-25 0.5531054040016272 1104 26-27 0.5573082847609996 1104 28-29 0.4115278466588066 1104 30-31 0.467942220384181 1104 32-33 0.5025302293557914 1104 34-35 0.5439027513877122 1104 36-37 0.441557372119874 1104 38-39 0.43713268751178447 1104 40-41 0.5470784649667664 1104 42-43 0.5965867167739134 1104 44-45 0.8635346456456077 1104 46-47 0.7071126581493701 1104 48-49 0.6369485690326755 1104 50-51 0.5062751017037073 1104 52-53 0.4527199826170758 1104 54-55 0.441452849049881 1104 56-57 0.4110607828958308 1104 58-59 0.4778023567082883 1104 60-61 0.38291588271761867 1104 62-63 0.7155020811679336 1104 64-65 0.7273434878145792 1104 66-67 0.6610545032425215 1104 68-69 0.7501876726191519 1104 70-71 0.7638197989945006 1104 72-73 0.9216133099650854 1104 74-75 0.712642655053422 1104 76-77 0.6396984505984591 1104 78-79 0.606163978122364 1104 80-81 0.8133655344718029 1104 82-83 0.9670263612726089 1104 84-85 1.0105706561098522 1104 86-87 0.7561689101145177 1104 88-89 0.9190270107799243 1104 90-91 0.8586466282105718 1104 92-93 1.0887502327605603 1104 94-95 1.1092709949090143 1104 96-97 1.2341489079781915 1104 98-99 1.1715518737063029 1104 100-101 1.2221854473308902 1105 1 -0.015415279509866764 1105 2 -0.04858751922669313 1105 3 0.09710957766242245 1105 4 0.20074361996573487 1105 5 0.1468622720524877 1105 6 -0.1484987215193314 1105 7 -0.220444151900125 1105 8 6.043079389641548E-4 1105 9 -0.2410709263051416 1105 10-11 0.05305599357190971 1105 12-13 0.1507909660515665 1105 14-15 -0.16833705324584258 1105 16-17 0.17022347728874365 1105 18-19 0.05865437928256512 1105 20-21 0.12888637092100197 1105 22-23 0.2660662280000068 1105 24-25 0.0762916741555486 1105 26-27 -0.0318206904865761 1105 28-29 -0.10070273497878901 1105 30-31 -0.22462958337361272 1105 32-33 -0.1674711529097408 1105 34-35 -0.5869274471648538 1105 36-37 -0.5377308598637711 1105 38-39 -0.24965458182265365 1105 40-41 -0.43234429107323535 1105 42-43 -0.16604990536405495 1105 44-45 -0.4555379035482403 1105 46-47 -0.3668188012268345 1105 48-49 -0.17567299628430533 1105 50-51 -0.2165843613848608 1105 52-53 -0.3449212799837582 1105 54-55 -0.22176185628072886 1105 56-57 0.005353507242460864 1105 58-59 0.027077654635874637 1105 60-61 -0.20873079046544518 1105 62-63 0.07152184756505875 1105 64-65 0.10555108435101346 1105 66-67 0.0880560731012352 1105 68-69 0.19721728272153527 1105 70-71 0.21237113534907337 1105 72-73 -0.01696473373425178 1105 74-75 0.10978111856654493 1105 76-77 -0.18137961778419154 1105 78-79 -0.1825616717892693 1105 80-81 -0.012808403898901588 1105 82-83 0.16739315530517018 1105 84-85 0.31163672840234113 1105 86-87 -0.11134957768698683 1105 88-89 0.03778460089869995 1105 90-91 0.08346471219307361 1105 92-93 0.28359783798552485 1105 94-95 0.3418260633805268 1105 96-97 0.21841699837835904 1105 98-99 -0.2373741059743928 1105 100-101 0.2989616585410637 1106 1 -0.02874688333783837 1106 2 0.31335471529423486 1106 3 0.08325912222601062 1106 4 0.37207995110836123 1106 5 0.37220240810690797 1106 6 0.020185701402375855 1106 7 0.16206478387757528 1106 8 0.16052590369591258 1106 9 0.07411701327746556 1106 10-11 0.09666114749789045 1106 12-13 0.00816442390928529 1106 14-15 0.3182232488411003 1106 16-17 0.19063164055404513 1106 18-19 0.08469784751731879 1106 20-21 0.4067674386023228 1106 22-23 0.49128385742465497 1106 24-25 0.541903342551727 1106 26-27 0.1751866887362965 1106 28-29 0.39669580070667365 1106 30-31 0.39664477382689967 1106 32-33 0.3781805823606348 1106 34-35 0.3570654042129817 1106 36-37 0.24430822695739352 1106 38-39 0.6689289532863043 1106 40-41 0.5955151577910556 1106 42-43 0.4695649337919505 1106 44-45 0.4827475801367669 1106 46-47 0.4803577812705697 1106 48-49 0.6685129831922296 1106 50-51 0.5336606518746763 1106 52-53 0.5669099779431477 1106 54-55 0.6147926807883408 1106 56-57 0.6069907723179853 1106 58-59 0.6299111609197467 1106 60-61 0.810971158110597 1106 62-63 1.0622401687918526 1106 64-65 0.9385529291567352 1106 66-67 1.0673452463976751 1106 68-69 0.7876560005815598 1106 70-71 0.8124302267822578 1106 72-73 0.8116052535827087 1106 74-75 1.110177463545213 1106 76-77 0.9414945318756729 1106 78-79 0.8603228549027655 1106 80-81 0.4798051669531702 1106 82-83 0.6920746380677656 1106 84-85 0.7142295451900722 1106 86-87 0.8839086951136572 1106 88-89 1.0858648499474732 1106 90-91 1.0956072810850408 1106 92-93 1.053724956629594 1106 94-95 1.1634347916175862 1106 96-97 1.1648455698069284 1106 98-99 0.998854707584929 1106 100-101 1.3102106496629347 1107 1 0.05262366020394893 1107 2 0.28557856099849843 1107 3 0.40059304001288254 1107 4 0.2512831461210183 1107 5 0.4466276954632278 1107 6 0.4047853507302577 1107 7 0.19268040826877098 1107 8 0.3952911481923067 1107 9 0.11724481378399787 1107 10-11 0.33424881552827657 1107 12-13 0.39435183867647794 1107 14-15 0.45100378653835094 1107 16-17 0.30701095089887076 1107 18-19 0.19492578439633945 1107 20-21 0.48383007253958255 1107 22-23 0.5416174829847549 1107 24-25 0.206159138382624 1107 26-27 -0.007459911692301091 1107 28-29 0.08028483246198448 1107 30-31 0.382771308017432 1107 32-33 0.5102792575992865 1107 34-35 0.3176878472287612 1107 36-37 0.375581361576522 1107 38-39 0.22099402244663935 1107 40-41 0.195963238835283 1107 42-43 0.22236787554140847 1107 44-45 0.355633806515101 1107 46-47 0.4484052194159034 1107 48-49 0.6387886504433524 1107 50-51 0.42178650420274977 1107 52-53 0.5095239463826502 1107 54-55 0.5657058894382487 1107 56-57 0.43834670452137914 1107 58-59 0.37072062906702996 1107 60-61 0.4846658785469842 1107 62-63 0.27308178188361154 1107 64-65 0.44425622157832123 1107 66-67 0.3637070231364241 1107 68-69 0.19071951119913422 1107 70-71 0.2040847173335898 1107 72-73 0.33661255925191114 1107 74-75 0.7403523125613845 1107 76-77 0.6453738422204971 1107 78-79 0.4116208287971723 1107 80-81 0.5419593652777372 1107 82-83 0.5149535587769023 1107 84-85 0.4206268761855938 1107 86-87 0.603634001951761 1107 88-89 0.582090254194501 1107 90-91 0.40618345486256047 1107 92-93 0.5473909414338003 1107 94-95 0.8521767553814641 1107 96-97 0.987259362910379 1107 98-99 0.6689477331060694 1107 100-101 0.3784772578842457 1108 1 0.052751037148720314 1108 2 0.09145609716445335 1108 3 0.22329646402647185 1108 4 0.06224362894030122 1108 5 0.42068525771112775 1108 6 0.11010788735112698 1108 7 0.029322509381799478 1108 8 -0.15563840022609554 1108 9 0.3261126753677175 1108 10-11 -0.11854892808012352 1108 12-13 -0.12060888676554526 1108 14-15 0.16263148192548726 1108 16-17 0.17249179886539423 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96-97 -0.12759474849545782 2316 98-99 -0.009920525072534048 2316 100-101 -0.797207976421678 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 906.0 3 308.0 4 289.0 5 342.0 6 374.0 7 448.0 8 636.0 9 805.0 10 1026.0 11 1537.0 12 2353.0 13 3205.0 14 3678.0 15 4991.0 16 6783.0 17 9854.0 18 8460.0 19 4150.0 20 3734.0 21 3630.0 22 3766.0 23 4016.0 24 4500.0 25 4866.0 26 5520.0 27 6397.0 28 7475.0 29 8890.0 30 10551.0 31 13130.0 32 16707.0 33 21938.0 34 30367.0 35 44791.0 36 63281.0 37 90422.0 38 83056.0 39 15636.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.09251952522448 18.654099079351163 15.346988812574084 19.906392582850277 2 14.996408118868942 21.197983676219312 41.65445697656957 22.151151228342176 3 18.75220696569107 28.432820979060715 31.15200564983217 21.662966405416046 4 11.605015444940108 17.33831522290641 38.860565272630595 32.19610405952289 5 12.045690339530548 38.74522113239113 33.40141675786191 15.80767177021641 6 27.736488338242726 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19.895886597352423 28-29 19.441459923034856 25.588627096478334 34.981397892336055 19.988515088150756 30-31 19.61820351116414 26.045800969100778 34.683601581078996 19.652393938656083 32-33 19.15761600154212 26.111469023136895 34.28217784113102 20.448737134189955 34-35 19.713470013879082 25.862268377608416 34.17490483495256 20.249356773559942 36-37 20.163119790271658 25.702679230887853 33.96671861176719 20.1674823670733 38-39 20.17095231089986 25.73202591170339 34.06425169050023 20.03277008689653 40-41 19.822838799743522 25.923344452831415 34.170339347602 20.083477399823064 42-43 19.54850373761231 26.53065572572987 33.36123353380896 20.559607002848864 44-45 19.658785620946862 26.905837330671144 32.76467652000292 20.67070052837907 46-47 19.869812591817023 27.010336263361662 31.67667015672811 21.443180988093207 48-49 20.258062683381155 27.25769338468344 30.980914156314803 21.503329775620607 50-51 20.73577543438164 27.737015220351157 30.515779370854272 21.01142997441293 52-53 20.425506011261604 27.956069598741944 30.460102470450973 21.15832191954548 54-55 19.903149517624534 27.774762261792556 30.741252157221226 21.580836063361684 56-57 20.430069344750446 28.365300231825536 29.627506581917608 21.57712384150641 58-59 20.44516996974573 27.67778695393681 30.119941642334062 21.757101433983397 60-61 20.54079171777616 27.313827007930975 30.6568698770024 21.48851139729046 62-63 21.19706542130035 27.267820382355595 30.450261610122286 21.084852586221768 64-65 20.90904940419725 27.413724008136803 30.727898990001474 20.949327597664475 66-67 20.503680704584774 27.7083238208301 30.376390727972847 21.41160474661228 68-69 20.48509889592514 28.02966116743157 30.483262322544903 21.001977614098386 70-71 20.45839701086019 28.083362254600512 29.903981392970003 21.5542593415693 72-73 20.433543343679954 27.944499633183735 29.522976498391184 22.098980524745134 74-75 20.436739270511474 28.029751848074806 29.877572971683993 21.655935909729727 76-77 20.539213596942556 27.798107972616144 29.945666624044293 21.71701180639701 78-79 20.26566004038682 27.741077861325053 29.993404162480847 21.99985793580728 80-81 20.088528091073705 28.46682581829107 29.717810365967534 21.72683572466769 82-83 20.014269519352652 28.392892845652717 29.441793859235148 22.151043775759476 84-85 20.066361915972013 27.78626186574396 29.136635532036877 23.010740686247154 86-87 20.252169139619433 27.76376950855835 29.41628303175249 22.567778320069728 88-89 20.55321169616277 27.109625652171264 29.594826868948836 22.74233578271713 90-91 20.03817549157795 27.260568532719603 30.183398633516294 22.51785734218615 92-93 19.84846316767999 27.735746231622404 29.783096924914577 22.63269367578303 94-95 19.579826390474306 27.800151185384735 29.564961518753584 23.055060905387375 96-97 19.343473447398623 27.72362929355762 29.3422658882815 23.590631370762257 98-99 19.524049224948598 27.861864872928916 29.20899493988962 23.405090962232862 100-101 19.918111240013843 28.137944194251347 29.00331797665338 22.940626589081432 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 655.0 1 484.5 2 391.0 3 486.5 4 437.0 5 420.0 6 717.0 7 1602.5 8 2740.0 9 3060.0 10 2368.5 11 1785.0 12 1742.0 13 1843.0 14 1913.5 15 1885.5 16 1875.5 17 1906.0 18 1953.0 19 1975.0 20 2058.5 21 2309.0 22 2627.5 23 2944.5 24 3250.0 25 3739.5 26 4390.5 27 4971.5 28 5531.0 29 6178.0 30 7000.5 31 7799.0 32 8682.0 33 9722.0 34 10731.0 35 11456.0 36 12501.0 37 13591.0 38 13850.5 39 13526.0 40 13371.0 41 13904.5 42 14215.0 43 14535.5 44 15278.0 45 15721.0 46 15864.0 47 16675.5 48 22508.0 49 22780.0 50 16788.0 51 14886.0 52 13585.5 53 13029.5 54 12625.5 55 11762.5 56 10714.0 57 9491.0 58 8580.0 59 7912.5 60 7154.0 61 6254.0 62 5215.5 63 3821.5 64 2535.5 65 1856.0 66 1333.0 67 1021.5 68 756.0 69 457.5 70 344.0 71 269.5 72 205.5 73 171.5 74 125.0 75 98.0 76 69.0 77 37.5 78 25.5 79 16.0 80 13.0 81 12.5 82 8.0 83 5.5 84 5.0 85 4.5 86 3.0 87 2.0 88 2.0 89 2.0 90 1.5 91 0.0 92 0.5 93 1.5 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026784192456597435 2 0.010145527445680846 3 0.014203738423953184 4 0.02232016038049786 5 0.05985861192951699 6 0.04768397899469998 7 0.020696875989188925 8 0.053974206011022104 9 0.029827850690301687 10-11 0.026682737182140627 12-13 0.04017628868489615 14-15 0.021914339282670628 16-17 0.01897213632342318 18-19 0.01704448610874382 20-21 0.014609559521780419 22-23 0.007507690309803826 24-25 0.0017247396657657436 26-27 0.0 28-29 0.0015218291168521269 30-31 0.0 32-33 1.0145527445680846E-4 34-35 0.0 36-37 0.0 38-39 1.0145527445680846E-4 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 4.0582109782723384E-4 50-51 2.0291054891361692E-4 52-53 6.087316467408508E-4 54-55 0.00131891856793851 56-57 1.0145527445680846E-4 58-59 0.0010145527445680846 60-61 0.0027392924103338285 62-63 0.0029422029592474455 64-65 0.001116008019024893 66-67 0.011261535464705739 68-69 0.012681909307101058 70-71 0.022523070929411478 72-73 0.01511683589406446 74-75 0.01734885193211425 76-77 0.026175460809856582 78-79 0.018870681048966373 80-81 0.043828678565341256 82-83 0.03439333804085806 84-85 0.015319746442978078 86-87 0.012884819856014675 88-89 0.010348437994594463 90-91 0.02100124181255935 92-93 0.02891475322019041 94-95 0.011261535464705739 96-97 0.019986689067991267 98-99 0.030639492885956156 100-101 0.04230684944848913 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 492828.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.999730704092535 #Duplication Level Percentage of deduplicated Percentage of total 1 83.09645681064133 52.3505440153466 2 10.367588819215221 13.063106073226393 3 2.911352568441906 5.502432833895245 4 1.172837416652092 2.955537656350615 5 0.6127519211421611 1.9301603010185753 6 0.39209172849523 1.4821003983900989 7 0.24827358652355797 1.0948818364346358 8 0.1758412121729386 0.8862359210861063 9 0.14854293626217996 0.8422348484261268 >10 0.7988097822815251 9.150392338923687 >50 0.045787850624483636 1.9353377586235814 >100 0.024828622838585543 3.0213370554009535 >500 0.0022571475307805026 1.1339409294189549 >1k 0.0019346978835261448 2.1279167387430227 >5k 6.44899294508715E-4 2.523841294715352 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7410 1.5035671674499014 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5014 1.0173934922528751 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3600 0.730477976089021 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1778 0.3607749559684109 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1503 0.3049745550171662 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1415 0.28711842671276794 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1094 0.2219841405114969 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1085 0.22015794557127435 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 925 0.18769225774509565 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 902 0.18302531512008247 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 897 0.18201076237551436 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 878 0.17815546194615567 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 743 0.15076253784281737 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 719 0.14589268466889058 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 518 0.10510766433725355 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 497 0.10084654281006761 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.0145527445680846E-4 0.0 22-23 0.0 0.0 0.0 5.072763722840423E-4 0.0 24-25 0.0 0.0 0.0 0.0014203738423953184 0.0 26-27 0.0 0.0 0.0 0.0022320160380497863 0.0 28-29 2.0291054891361692E-4 0.0 0.0 0.004666942625013189 0.0 30-31 4.0582109782723384E-4 0.0 0.0 0.009841161622310421 0.0 32-33 4.0582109782723384E-4 0.0 0.0 0.0131891856793851 0.0 34-35 8.116421956544677E-4 0.0 0.0 0.016131388638632546 0.0 36-37 0.0012174632934817016 0.0 0.0 0.021508518184843395 0.0 38-39 0.0012174632934817016 0.0 0.0 0.029219119043560837 0.0 40-41 0.0012174632934817016 0.0 0.0 0.033987516943030836 0.0 42-43 0.0013189185679385101 0.0 0.0 0.051336368875145075 0.0 44-45 0.0018261949402225523 0.0 0.0 0.06909104190508657 0.0 46-47 0.0018261949402225523 0.0 0.0 0.08694717020948485 0.0 48-49 0.0018261949402225523 0.0 0.0 0.10591930653290804 0.0 50-51 0.0018261949402225523 0.0 0.0 0.13341368591070313 0.0 52-53 0.0018261949402225523 1.0145527445680846E-4 0.0 0.19256211091902248 0.0 54-55 0.0018261949402225523 2.0291054891361692E-4 0.0 0.2339558628974003 0.0 56-57 0.0018261949402225523 2.0291054891361692E-4 0.0 0.3107575056612043 0.0 58-59 0.0018261949402225523 2.0291054891361692E-4 0.0 0.4037919923380977 0.0 60-61 0.0019276502146793608 2.0291054891361692E-4 0.0 0.5079865592052399 0.0 62-63 0.0020291054891361693 2.0291054891361692E-4 0.0 0.659459283969255 0.0 64-65 0.0020291054891361693 2.0291054891361692E-4 0.0 0.7278401389531439 0.0 66-67 0.0022320160380497863 2.0291054891361692E-4 0.0 0.8910816755541487 0.0 68-69 0.0022320160380497863 2.0291054891361692E-4 0.0 1.1068770443237803 0.0 70-71 0.0023334713125065945 2.0291054891361692E-4 0.0 1.2432329331937308 0.0 72-73 0.0024349265869634032 2.0291054891361692E-4 0.0 1.5253800514581153 0.0 74-75 0.0024349265869634032 2.0291054891361692E-4 0.0 1.8018456743529183 0.0 76-77 0.0024349265869634032 2.0291054891361692E-4 0.0 1.9350564497147078 0.0 78-79 0.00263783713587702 2.0291054891361692E-4 0.0 2.0702963305656334 0.0 80-81 0.003348024057074679 2.0291054891361692E-4 0.0 2.376082527778454 0.0 82-83 0.0034494793315314877 2.0291054891361692E-4 0.0 2.618966454828054 0.0 84-85 0.0035509346059882964 2.0291054891361692E-4 0.0 3.0463975261145877 0.0 86-87 0.0036523898804451047 2.0291054891361692E-4 0.0 3.6025753406868115 0.0 88-89 0.0036523898804451047 2.0291054891361692E-4 0.0 4.38144748269173 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAATAC 150 9.440555E-10 31.69003 3 GTATCAA 6925 0.0 31.235584 1 GGTATCA 4585 0.0 29.861467 1 GTACATG 4540 0.0 26.387768 1 GATTCGT 45 8.955852E-4 26.384235 66-67 TACATGG 4560 0.0 25.953997 2 TATCAAC 8340 0.0 25.816772 2 ACATGGG 4465 0.0 25.44429 3 ATCAACG 8500 0.0 25.277458 3 GAGTACT 4440 0.0 25.15165 12-13 TCAACGC 8590 0.0 25.012619 4 CAGACGC 95 0.0012224705 25.010822 9 CAACGCA 8615 0.0 24.940033 5 AACGCAG 8695 0.0 24.869524 6 ATGGGAG 1065 0.0 24.548615 5 CATGGGA 3330 0.0 24.124392 4 AGTACTT 4470 0.0 24.026056 12-13 TATGGGC 100 0.0016452299 23.767523 4 GTACTTT 4795 0.0 23.237623 14-15 ACGCAGA 9330 0.0 23.075027 7 >>END_MODULE