FastQCFastQC Report
Thu 26 May 2016
SRR936973_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936973_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492828
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76141.5449609194282792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59181.2008246284707849No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41920.8506010210458822No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA26200.5316256381536764No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18570.37680488933258666No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12930.2623633397453067No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12770.2591167709626888No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11600.23537623673979563No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT11210.22746272533216458No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10630.21569391349517478No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG8460.1716623243809199No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA7550.15319746442978077No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT6770.13737044161451867No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG6770.13737044161451867No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.12742782471775144No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT5920.12012304495686121No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5670.1150502812340208No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5250.10652803817964888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTG651.3274008E-436.536987
AGAATCG702.0521255E-433.9271935
GGGCGTA600.003949565331.6653829
GGTGTGC2450.027.1417548
GAATCGT908.942382E-426.387826
GTATCAA93400.026.090991
ATAGACT950.001225850224.9989858
GAGTACT52450.024.45086712-13
AGTACTT53200.024.24008812-13
GTACTAC1000.001652700523.7490356
GTACTTT55950.022.87887414-15
TAGACTA1050.002194971322.618135
GTACATG46400.022.2157941
GGTATCA67400.022.2009071
ATCAACG110750.022.1277833
TATCAAC110500.022.0918852
TCAACGC111350.022.0511994
GGTAAGG1302.9410332E-421.9199623
CAACGCA112450.021.8377085
TACTTTT58600.021.76319714-15
Produced by FastQC (version 0.11.5)