##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936973_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 492828 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21800709375279 33.0 31.0 34.0 31.0 34.0 2 32.309580624477505 33.0 31.0 34.0 31.0 34.0 3 32.38256551981625 34.0 31.0 34.0 30.0 34.0 4 36.0618978629461 37.0 35.0 37.0 35.0 37.0 5 35.9908101812397 37.0 35.0 37.0 35.0 37.0 6 36.064296265634255 37.0 35.0 37.0 35.0 37.0 7 36.011113410764 37.0 35.0 37.0 35.0 37.0 8 35.99391674174357 37.0 35.0 37.0 35.0 37.0 9 37.64248175834165 39.0 37.0 39.0 35.0 39.0 10-11 37.57459093233339 39.0 37.0 39.0 35.0 39.0 12-13 37.46610176369849 39.0 37.0 39.0 35.0 39.0 14-15 38.5444150900517 40.0 38.0 41.0 34.0 41.0 16-17 38.497637106657905 40.0 38.0 41.0 34.0 41.0 18-19 38.751676041134026 40.0 38.0 41.0 35.0 41.0 20-21 38.816225944954425 40.0 38.0 41.0 35.0 41.0 22-23 38.728584820667656 40.0 38.0 41.0 35.0 41.0 24-25 38.598534377105196 40.0 38.0 41.0 34.0 41.0 26-27 38.36716866736468 40.0 38.0 41.0 34.0 41.0 28-29 38.23482736370498 40.0 38.0 41.0 34.0 41.0 30-31 38.09504837387486 40.0 37.0 41.0 34.0 41.0 32-33 37.77730364346182 40.0 36.0 41.0 33.0 41.0 34-35 37.55235599438343 40.0 36.0 41.0 33.0 41.0 36-37 37.31131145145974 40.0 35.0 41.0 33.0 41.0 38-39 37.08584638048163 39.5 35.0 41.0 33.0 41.0 40-41 36.82449657892815 39.0 35.0 41.0 32.0 41.0 42-43 36.45373030753123 39.0 35.0 41.0 31.0 41.0 44-45 35.9520177424984 39.0 35.0 41.0 30.0 41.0 46-47 35.254372722329094 39.0 35.0 41.0 26.0 41.0 48-49 34.62175140211189 39.0 35.0 40.0 22.5 41.0 50-51 34.216777455826374 38.5 34.0 40.0 20.0 41.0 52-53 33.902824109019775 38.0 34.0 40.0 18.0 41.0 54-55 33.58068332156452 38.0 34.0 40.0 13.0 41.0 56-57 33.30314125820773 38.0 33.0 40.0 10.5 41.0 58-59 32.966291485061724 37.0 33.0 40.0 8.0 41.0 60-61 32.62233781359825 37.0 33.0 40.0 6.5 41.0 62-63 32.24909096074087 36.0 32.0 39.0 2.0 41.0 64-65 31.84411092713888 36.0 31.5 39.0 2.0 40.0 66-67 31.455797966024658 35.0 31.0 39.0 2.0 40.0 68-69 31.16215393605883 35.0 31.0 38.0 2.0 40.0 70-71 30.70703572037303 35.0 30.5 37.5 2.0 40.0 72-73 30.193370709456445 35.0 30.0 37.0 2.0 39.0 74-75 29.68609636627789 34.0 29.0 36.5 2.0 39.0 76-77 28.287798177051627 32.5 27.0 35.0 2.0 37.5 78-79 28.954844286444764 34.0 29.0 35.0 2.0 37.5 80-81 28.669295372827843 34.0 29.0 35.0 2.0 37.0 82-83 28.395110464502828 34.0 29.0 35.0 2.0 37.0 84-85 28.08610204777326 34.0 28.0 35.0 2.0 36.0 86-87 27.740870039851632 34.0 27.0 35.0 2.0 36.0 88-89 27.34684210312726 33.5 26.5 35.0 2.0 35.5 90-91 26.912428879852605 33.0 25.5 35.0 2.0 35.0 92-93 26.462509232429976 33.0 25.0 35.0 2.0 35.0 94-95 25.90911129237787 33.0 24.0 35.0 2.0 35.0 96-97 25.23727852313586 32.0 19.5 34.0 2.0 35.0 98-99 24.504974352106615 32.0 9.5 34.0 2.0 35.0 100-101 21.384942616896765 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.014049300844590107 1101 2 0.04719792227461994 1101 3 0.03415855223337161 1101 4 0.032000560497330355 1101 5 0.0519549879880401 1101 6 0.07488146133720619 1101 7 0.11081108768301817 1101 8 0.11252050434641347 1101 9 0.16787981460355894 1101 10-11 0.18771501992028305 1101 12-13 0.14219776166505937 1101 14-15 0.22082197122949765 1101 16-17 0.22445559454710207 1101 18-19 0.004216022509851314 1101 20-21 0.1374544824948245 1101 22-23 0.13695917698260018 1101 24-25 0.06124785331282823 1101 26-27 0.026303118907819112 1101 28-29 0.151671233115529 1101 30-31 0.1258443310555819 1101 32-33 0.09714788675970709 1101 34-35 0.12955800468757417 1101 36-37 0.2530306829978599 1101 38-39 0.4429820628434271 1101 40-41 0.4387605670017294 1101 42-43 0.6489901092306667 1101 44-45 0.6851749130796989 1101 46-47 0.7656096786492839 1101 48-49 0.8884926654901832 1101 50-51 0.7791863766871074 1101 52-53 0.8206049178884882 1101 54-55 0.9347270078981893 1101 56-57 0.8740929353982594 1101 58-59 0.7353856733870217 1101 60-61 1.049643625551191 1101 62-63 0.8788870984825152 1101 64-65 0.8267297751328861 1101 66-67 0.6944534591774989 1101 68-69 0.7724223564617105 1101 70-71 0.9700204609709431 1101 72-73 1.038492481428161 1101 74-75 1.1414339808349538 1101 76-77 1.0329587511995726 1101 78-79 0.8670873523142824 1101 80-81 1.140019138277406 1101 82-83 1.3556424252410366 1101 84-85 1.1045290409598287 1101 86-87 0.6995240283532382 1101 88-89 0.6333835154145824 1101 90-91 0.9124064982791431 1101 92-93 0.9905789782990553 1101 94-95 1.1240438408411215 1101 96-97 0.7080389252567336 1101 98-99 1.0882972725179734 1101 100-101 1.236810748360348 1102 1 0.075028335571389 1102 2 0.09414159828882873 1102 3 0.12363835226987874 1102 4 0.02279929106618539 1102 5 0.07651072219387345 1102 6 -0.04202573959521061 1102 7 0.04189635301378303 1102 8 0.05124131374918761 1102 9 0.13752106690299826 1102 10-11 0.18311526285345536 1102 12-13 0.037198498890006704 1102 14-15 0.19770689743679526 1102 16-17 0.1632448605519059 1102 18-19 0.0029938966339599915 1102 20-21 0.08745044531060131 1102 22-23 0.0767779249640057 1102 24-25 0.059660625559466496 1102 26-27 -0.10882143700191449 1102 28-29 0.0020536246732447694 1102 30-31 0.1193159408740101 1102 32-33 0.17005504556497897 1102 34-35 0.2911032983839448 1102 36-37 0.04347888065840522 1102 38-39 0.11923850478520848 1102 40-41 0.03986789642191724 1102 42-43 0.013333698182115938 1102 44-45 0.16076530738972394 1102 46-47 0.35528168376071534 1102 48-49 0.3481568068688645 1102 50-51 0.3489739330321555 1102 52-53 0.24627465164496698 1102 54-55 0.22969290246799545 1102 56-57 0.20820328041953218 1102 58-59 0.36288653348278643 1102 60-61 0.5994602847285933 1102 62-63 0.5345104572793602 1102 64-65 0.5044431139895487 1102 66-67 0.45367359805663554 1102 68-69 0.4946645208137248 1102 70-71 0.4375180562133849 1102 72-73 0.5417125746695639 1102 74-75 0.5167674873576402 1102 76-77 0.18255003017953442 1102 78-79 0.34178868844673005 1102 80-81 0.7552381818509097 1102 82-83 0.6699490651803721 1102 84-85 0.4994762416113936 1102 86-87 0.28732313104516294 1102 88-89 0.4649491179028935 1102 90-91 0.35723618176390204 1102 92-93 0.4709415876540852 1102 94-95 0.5579876081308051 1102 96-97 0.7319710900300187 1102 98-99 0.45982134466771996 1102 100-101 0.5063989906756561 1103 1 0.18510739544392862 1103 2 0.13028373876083776 1103 3 0.11899694768544578 1103 4 0.17437065407466434 1103 5 0.004258092328363716 1103 6 0.049908209125518965 1103 7 0.002642233286799467 1103 8 -0.04606992713134872 1103 9 0.051856292518664304 1103 10-11 0.03762599369946429 1103 12-13 0.12230259702643309 1103 14-15 0.3742255274876811 1103 16-17 0.43460538216168487 1103 18-19 0.23777311905738685 1103 20-21 0.24590478749945532 1103 22-23 0.15940136982217723 1103 24-25 0.08801660071789996 1103 26-27 0.16526785973372426 1103 28-29 0.2603580763974449 1103 30-31 0.2782518316144902 1103 32-33 0.2718488704395696 1103 34-35 0.24739598495009574 1103 36-37 0.2542383256793457 1103 38-39 0.19122549167035174 1103 40-41 0.4921832858545301 1103 42-43 0.41575737951906433 1103 44-45 0.3748656367870211 1103 46-47 0.1612673548653163 1103 48-49 0.3453828159165582 1103 50-51 0.6828973329005805 1103 52-53 0.5410196682956965 1103 54-55 0.5795712718948636 1103 56-57 0.7114424291863841 1103 58-59 0.8246085104091563 1103 60-61 0.7211897674821515 1103 62-63 0.7595191048061665 1103 64-65 0.6727104209123205 1103 66-67 0.6076159659642535 1103 68-69 0.6232685047801922 1103 70-71 0.675897588817385 1103 72-73 0.9123766595125389 1103 74-75 1.0484993891579073 1103 76-77 0.899228792079775 1103 78-79 1.0951691366712168 1103 80-81 1.055513094075689 1103 82-83 0.983853157890902 1103 84-85 0.8748693367025417 1103 86-87 0.6716045272194506 1103 88-89 0.6875607614303583 1103 90-91 0.912365578155228 1103 92-93 0.7060124521580811 1103 94-95 0.8710037750495481 1103 96-97 0.7633260048773174 1103 98-99 0.6151817939321731 1103 100-101 0.8894827326470178 1104 1 0.2612027586012857 1104 2 0.32993060282818476 1104 3 0.20095212937108897 1104 4 0.19339490625255706 1104 5 0.20173447039773862 1104 6 0.13622904612021358 1104 7 0.17508314966302407 1104 8 0.20746060941805666 1104 9 0.14085938286946487 1104 10-11 0.1982592358690951 1104 12-13 0.1650327030988592 1104 14-15 0.0673584652297805 1104 16-17 0.2603823305801356 1104 18-19 0.31485005529874144 1104 20-21 0.2371537302308795 1104 22-23 0.19261112203462005 1104 24-25 0.2275003574523211 1104 26-27 0.28378231921853825 1104 28-29 0.1920511212980358 1104 30-31 0.38169136901629486 1104 32-33 0.39038389935195283 1104 34-35 0.0948654516918026 1104 36-37 0.2171154455645521 1104 38-39 0.22866349541563835 1104 40-41 0.028806740267739883 1104 42-43 -0.08446723863008287 1104 44-45 -0.2640849603846007 1104 46-47 0.0014165737207321172 1104 48-49 0.10847237684978239 1104 50-51 -0.1537978676760261 1104 52-53 -0.24138211680151045 1104 54-55 -0.26505796828693917 1104 56-57 -0.27602256164787775 1104 58-59 -0.2625260531405047 1104 60-61 0.028471509400006312 1104 62-63 0.16934108875214093 1104 64-65 0.04529515872616585 1104 66-67 -0.2876387493713253 1104 68-69 -0.14381481621148495 1104 70-71 -0.04729962427200718 1104 72-73 -0.09798950920942318 1104 74-75 -0.14096396270436884 1104 76-77 -0.15101820560657586 1104 78-79 0.0023664616583012332 1104 80-81 0.4467666443588847 1104 82-83 0.33738073244273536 1104 84-85 0.39435763516658184 1104 86-87 -0.03169875829407687 1104 88-89 0.03109899917344805 1104 90-91 0.22655782496202903 1104 92-93 0.09840390317487291 1104 94-95 0.05354592592054175 1104 96-97 0.18625482807567906 1104 98-99 0.5522689655206925 1104 100-101 0.8503670863885162 1105 1 0.06662666982034438 1105 2 0.04128147118714054 1105 3 -0.06751098882249096 1105 4 0.06325954296355718 1105 5 -0.0229527920253787 1105 6 0.05614403678991664 1105 7 0.008878060951197142 1105 8 0.06900943794801861 1105 9 0.06206037415132215 1105 10-11 0.061152070796971714 1105 12-13 0.06306223421464097 1105 14-15 0.10920285175072308 1105 16-17 0.19707703749048022 1105 18-19 -0.03575295804011347 1105 20-21 0.0049750822840337605 1105 22-23 0.015126993404933842 1105 24-25 -0.034148932161919276 1105 26-27 0.20863520667250413 1105 28-29 -0.031592036981244576 1105 30-31 0.021165663814038282 1105 32-33 -0.028888091011680217 1105 34-35 0.09688578086846888 1105 36-37 0.20350136422809584 1105 38-39 0.3989919315796584 1105 40-41 0.4695075488930698 1105 42-43 0.5331043182945692 1105 44-45 0.7402284485783994 1105 46-47 1.0858705294684938 1105 48-49 1.0401107070730191 1105 50-51 0.8682714825604378 1105 52-53 0.6711217091120218 1105 54-55 0.9500361245025744 1105 56-57 0.7644469643337786 1105 58-59 0.6451867417016715 1105 60-61 0.3969267289333871 1105 62-63 0.7067980163707901 1105 64-65 0.5153974957422527 1105 66-67 0.23913524034066924 1105 68-69 0.2797310898142058 1105 70-71 0.14925359788767878 1105 72-73 0.21112949397966219 1105 74-75 0.3481025637610813 1105 76-77 0.5644782251863525 1105 78-79 0.4766884110476326 1105 80-81 0.44015028228430353 1105 82-83 0.5470617746709472 1105 84-85 0.5307650283125191 1105 86-87 0.25607164740085864 1105 88-89 0.3383544122240103 1105 90-91 0.3420707935747309 1105 92-93 0.1365680077136382 1105 94-95 0.36986998820147576 1105 96-97 0.1340743041970427 1105 98-99 0.03524982114305786 1105 100-101 -0.11646964830536177 1106 1 -0.0694133394036811 1106 2 -0.11996607868026388 1106 3 -0.10492595480888411 1106 4 -0.027798914317664014 1106 5 0.014073035254689614 1106 6 -0.010744583064798974 1106 7 -0.041104643995190315 1106 8 -0.10653535624084043 1106 9 -0.20295749740407842 1106 10-11 -0.16141365040676448 1106 12-13 -0.10917488097041428 1106 14-15 0.07227070601106789 1106 16-17 -0.05759262698547474 1106 18-19 -0.2634567514218773 1106 20-21 -0.07163479895659464 1106 22-23 -0.04448329138693907 1106 24-25 0.09069252984661347 1106 26-27 -0.10508265335286637 1106 28-29 0.16076499057554372 1106 30-31 -0.07840340110006849 1106 32-33 0.1380884453655824 1106 34-35 0.21009631932113138 1106 36-37 0.18542803320077184 1106 38-39 0.39946033928171687 1106 40-41 0.32513708884464876 1106 42-43 0.07732921164174655 1106 44-45 0.1408251279281032 1106 46-47 0.4675075062931171 1106 48-49 0.2950037659915026 1106 50-51 0.27201730292248527 1106 52-53 0.3993155290128598 1106 54-55 0.2924421547112672 1106 56-57 0.5255013861024835 1106 58-59 0.5898930140411167 1106 60-61 -0.01862296552065601 1106 62-63 0.2633240166013948 1106 64-65 0.05597601528566187 1106 66-67 0.04919952028993535 1106 68-69 0.352341489906447 1106 70-71 0.4435001100518683 1106 72-73 0.4188935317981759 1106 74-75 0.34185472564278285 1106 76-77 0.2649278965803319 1106 78-79 0.35736695365111615 1106 80-81 0.5278578229276789 1106 82-83 0.6778628239042028 1106 84-85 0.8653162216712182 1106 86-87 0.7609647063193421 1106 88-89 0.923510932459223 1106 90-91 1.115087627426913 1106 92-93 1.1606225447827008 1106 94-95 1.1184935901846416 1106 96-97 1.2360214389831619 1106 98-99 0.8017954224344201 1106 100-101 0.8726128491003813 1107 1 0.13855958091581755 1107 2 0.09566805340623574 1107 3 0.08415450496123356 1107 4 -0.05818075729447969 1107 5 0.0269651061683831 1107 6 0.0892310647045349 1107 7 0.0024858488939614176 1107 8 -0.08624507765132705 1107 9 0.041558380250315 1107 10-11 -0.02059200614414891 1107 12-13 -0.007949963767998725 1107 14-15 0.13706468613964518 1107 16-17 0.14423866076047887 1107 18-19 0.026110752961521655 1107 20-21 -0.10772724002419665 1107 22-23 0.13842631313282538 1107 24-25 0.01793293506772642 1107 26-27 0.2630159244768677 1107 28-29 0.28312764399459667 1107 30-31 0.3435501348438308 1107 32-33 0.20203496786703568 1107 34-35 0.3802015209576055 1107 36-37 0.5388227341553815 1107 38-39 0.6723733531137839 1107 40-41 0.7517735587452918 1107 42-43 0.47068348789344583 1107 44-45 0.5467086268566064 1107 46-47 0.38501432491770515 1107 48-49 0.25985619147368055 1107 50-51 0.4199838916620138 1107 52-53 0.5210572005499685 1107 54-55 0.6694414728488098 1107 56-57 0.8467657539185751 1107 58-59 0.8287957025273798 1107 60-61 0.7984162902751706 1107 62-63 0.5808186591106477 1107 64-65 0.5238481173702176 1107 66-67 0.7754828828459459 1107 68-69 0.38313245035842414 1107 70-71 0.5531241116104084 1107 72-73 0.5344229492928179 1107 74-75 1.0792719280585246 1107 76-77 1.0405220910085404 1107 78-79 0.7518584790748477 1107 80-81 1.2074092548388435 1107 82-83 1.2108138272161035 1107 84-85 0.8209245403932108 1107 86-87 0.9490929938704795 1107 88-89 0.9881198356347554 1107 90-91 0.9673816075554917 1107 92-93 1.0027713856165228 1107 94-95 0.925757860591812 1107 96-97 0.7017261925385867 1107 98-99 0.47424817910693307 1107 100-101 0.9158843277678272 1108 1 0.11589255031502432 1108 2 0.04251547402310507 1108 3 -0.0726040550727376 1108 4 0.14362678220753367 1108 5 0.11965306298153422 1108 6 0.11522809257581912 1108 7 0.04564237089680745 1108 8 -0.03530643087972152 1108 9 0.017672170320977898 1108 10-11 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74-75 -0.4646714848504061 2316 76-77 -0.29060080907987285 2316 78-79 -0.36803542012939516 2316 80-81 -0.7443148830921906 2316 82-83 -0.42614461663889003 2316 84-85 -0.7651939388287126 2316 86-87 -1.0028963960499446 2316 88-89 -1.2730649565845198 2316 90-91 -0.6713451828954327 2316 92-93 -0.7275557585037227 2316 94-95 -0.4332164983825457 2316 96-97 -0.32418008509182883 2316 98-99 -0.4810126520159663 2316 100-101 -0.9294737624178495 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 7.0 5 16.0 6 45.0 7 75.0 8 191.0 9 397.0 10 677.0 11 1202.0 12 1879.0 13 2746.0 14 3326.0 15 4977.0 16 7241.0 17 10488.0 18 9269.0 19 4770.0 20 3512.0 21 3322.0 22 3344.0 23 3632.0 24 4116.0 25 4696.0 26 5524.0 27 6594.0 28 8013.0 29 9708.0 30 11887.0 31 14700.0 32 18468.0 33 24288.0 34 33681.0 35 48562.0 36 69865.0 37 99099.0 38 68032.0 39 4476.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.04210389578758 19.62040731825138 15.456294678557947 18.881194107403093 2 15.667530422536927 22.221793838992387 40.497434097185725 21.61324164128496 3 19.40514743480484 28.68871086870064 31.250253638186145 20.655888058308374 4 12.278978787645132 18.139870867202625 37.93894802628108 31.642202318871167 5 12.20912772813233 39.82018067155275 32.51093687858644 15.459754721728473 6 28.091950944345694 38.33102015307572 17.6491595445064 15.927869358072188 7 25.87231244977964 37.035436298262276 20.22470314186694 16.867548110091146 8 27.113922098582062 33.039315947957505 19.384044737717822 20.46271721574261 9 28.67978280454844 16.482423888253102 20.5921741459495 34.24561916124895 10-11 24.584478258928392 28.15086229639942 25.84200229729236 21.422657147379827 12-13 27.744365174056668 26.45568027790629 27.006379507657847 18.7935750403792 14-15 24.205909566826563 25.994870421323462 28.58786432589057 21.2113556859594 16-17 20.022299869325607 29.734410382526967 28.782759908122102 21.460529840025323 18-19 19.85863164686928 26.54912687236469 34.42198666466461 19.170254816101423 20-21 21.917345410716244 24.960330504725846 34.41374370462358 18.708580379934336 22-23 21.017201250778694 25.501762331756673 34.201816916355696 19.27921950110894 24-25 20.675505787165044 25.617451496281745 34.78173960953505 18.925303107018163 26-27 20.795179329314756 25.52149019520307 34.71589070089053 18.96743977459164 28-29 19.87784772562408 25.82272701908883 35.01509148738656 19.284333767900534 30-31 20.00478879584105 26.260254820754962 34.72536458766641 19.009591795737567 32-33 19.803231851400174 26.18241992153218 34.38582749863792 19.62852072842972 34-35 20.264575064728465 25.821178991453408 34.391004569545565 19.523241374272565 36-37 20.691981838013344 25.820764528321032 34.211703827714786 19.27554980595084 38-39 20.87632657989717 25.741763510339183 34.290016569882184 19.091893339881462 40-41 20.35501661857628 26.097962347460136 34.23132490026825 19.315696133695333 42-43 20.198426225782626 26.687099758942267 33.52873619193715 19.585737823337958 44-45 20.42152637431315 27.13654662478593 32.62324786741013 19.81867913349079 46-47 20.711569600629836 27.27220154780795 31.601112765966082 20.415116085596132 48-49 21.18962464679055 27.49904880758519 30.8587111469605 20.452615398663767 50-51 21.642333951924414 27.983315289684274 30.245755514812195 20.128595243579117 52-53 21.185586046247373 28.35522738156111 30.383622683776085 20.07556388841543 54-55 20.635052692774796 28.199537549499347 30.440667235513924 20.72474252221193 56-57 21.177298977691017 28.820164527217607 29.42374889916113 20.578787595930244 58-59 21.109718917876943 28.02380143153537 29.893725492185403 20.97275415840228 60-61 21.10698628594271 27.729444386388007 30.512331694112703 20.651237633556573 62-63 21.839741716586307 27.61824461436062 30.3263851685904 20.21562850046267 64-65 21.448963938737247 27.87848904688857 30.502528265439466 20.17001874893472 66-67 21.037866597403863 28.133211852867944 30.18918079824029 20.6397407514879 68-69 21.274916324168135 28.539469933008604 30.002262468688144 20.183351274135113 70-71 21.22358579771195 28.591357528681755 29.481131118334815 20.70392555527148 72-73 21.12177067861404 28.434058129814055 29.19375522494663 21.250415966625273 74-75 21.230733643380653 28.639302150040173 29.39981088736841 20.73015331921076 76-77 21.35227706217991 28.35035752838719 29.50512146225458 20.792243947178328 78-79 21.11606454388286 28.200104705242566 29.484773098057676 21.199057652816897 80-81 20.870065450723246 29.12744882191742 29.263301431474588 20.73918429588475 82-83 21.046671273619513 28.922023771470784 28.824506553745582 21.206798401164118 84-85 20.936348969589964 28.574878491776 28.584416506854183 21.904356031779855 86-87 21.054479264112924 28.315337271430398 28.84933430466748 21.780849159789202 88-89 21.313339860295947 27.794005001750104 29.05144294910491 21.841212188849042 90-91 20.834292937952554 28.062626756583732 29.48443107028973 21.618649235173983 92-93 20.391862044756117 28.483147720343773 29.195974648278856 21.929015586621254 94-95 20.316723075799263 28.82263183098363 28.69733456702947 22.163310526187637 96-97 20.358392610562053 28.540019865607846 28.4917255380607 22.609861985769406 98-99 20.37902815063726 28.812551616941924 28.25858895767766 22.54983127474316 100-101 20.582398584484523 29.25456146616236 28.143617193967824 22.01942275538529 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1143.0 1 796.0 2 493.5 3 561.5 4 481.5 5 456.0 6 782.0 7 1731.5 8 2818.5 9 3098.5 10 2490.0 11 1871.5 12 1784.5 13 1837.0 14 1920.5 15 1971.0 16 1956.5 17 1931.5 18 2001.0 19 2121.0 20 2198.5 21 2399.5 22 2716.0 23 3104.0 24 3384.5 25 3716.5 26 4363.5 27 5091.5 28 5673.0 29 6304.5 30 7063.0 31 7797.0 32 8587.5 33 9499.5 34 10652.0 35 11314.0 36 12052.0 37 13100.5 38 13406.0 39 13345.5 40 13250.5 41 13622.0 42 14188.0 43 14485.5 44 15103.0 45 15826.5 46 16039.5 47 16663.5 48 23018.5 49 23006.0 50 16286.5 51 14370.0 52 13098.5 53 12578.0 54 12248.5 55 11563.0 56 10518.5 57 9461.0 58 8898.5 59 8370.5 60 7542.0 61 6470.5 62 5268.5 63 3882.0 64 2585.5 65 1880.5 66 1347.5 67 1062.0 68 804.5 69 501.0 70 350.5 71 265.5 72 230.0 73 206.0 74 137.5 75 89.5 76 60.5 77 33.5 78 24.0 79 19.0 80 11.0 81 6.0 82 4.5 83 4.0 84 2.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0288132979457336 2 0.0038553004293587216 3 0.0 4 4.0582109782723384E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.015015380619607652 12-13 0.0 14-15 0.0 16-17 0.0 18-19 4.0582109782723384E-4 20-21 0.0012174632934817016 22-23 0.0030436582337042537 24-25 0.011464446013619355 26-27 0.006797503388606166 28-29 1.0145527445680846E-4 30-31 0.002232016038049786 32-33 0.0029422029592474455 34-35 0.0 36-37 0.008217877231001485 38-39 0.013493551502755526 40-41 0.0012174632934817016 42-43 0.0 44-45 0.0 46-47 4.0582109782723384E-4 48-49 0.005174218997297231 50-51 0.008420787779915102 52-53 0.0 54-55 0.003753845154901913 56-57 0.004464032076099572 58-59 1.0145527445680846E-4 60-61 0.0025363818614202115 62-63 0.007304779760890209 64-65 0.0 66-67 0.0038553004293587216 68-69 7.101869211976593E-4 70-71 0.0012174632934817016 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0032465687826178707 80-81 0.0029422029592474455 82-83 0.019175046872336798 84-85 0.012783364581557866 86-87 0.007101869211976592 88-89 1.0145527445680846E-4 90-91 7.101869211976593E-4 92-93 3.043658233704254E-4 94-95 0.0 96-97 0.0033480240570746794 98-99 0.0030436582337042537 100-101 8.116421956544677E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 492828.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.04844556421538 #Duplication Level Percentage of deduplicated Percentage of total 1 81.72829023331063 49.076567871313486 2 10.95654634502358 13.158471535419029 3 3.2189370637498547 5.7987650114165525 4 1.3552628997433627 3.2552572184176003 5 0.705911812383927 2.1194453519536425 6 0.43738221383999853 1.5758473235116297 7 0.29419293278057773 1.2366079816615978 8 0.20000676442143858 0.9608076244668482 9 0.15098899432012533 0.8159988965604903 >10 0.8568078885397055 9.453236517640093 >50 0.06131147563752202 2.563000935385991 >100 0.028297603969990606 3.2331841957378886 >500 0.002695009901903867 1.0654558692955929 >1k 0.002695009901903867 2.949979673806271 >5k 6.737524754759668E-4 2.7373739934133208 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7614 1.5449609194282792 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5918 1.2008246284707849 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4192 0.8506010210458822 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2620 0.5316256381536764 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1857 0.37680488933258666 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1293 0.2623633397453067 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1277 0.2591167709626888 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1160 0.23537623673979563 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1121 0.22746272533216458 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1063 0.21569391349517478 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 846 0.1716623243809199 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 755 0.15319746442978077 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 677 0.13737044161451867 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 677 0.13737044161451867 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 628 0.12742782471775144 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 592 0.12012304495686121 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 567 0.1150502812340208 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 525 0.10652803817964888 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 2.0291054891361692E-4 0.0 0.0 0.0 0.0 16-17 2.0291054891361692E-4 0.0 0.0 0.0 0.0 18-19 2.0291054891361692E-4 0.0 0.0 0.0 0.0 20-21 2.0291054891361692E-4 0.0 0.0 1.0145527445680846E-4 0.0 22-23 4.0582109782723384E-4 0.0 0.0 5.072763722840423E-4 0.0 24-25 4.0582109782723384E-4 0.0 0.0 0.0014203738423953184 0.0 26-27 4.0582109782723384E-4 0.0 0.0 0.002130560763592978 0.0 28-29 4.0582109782723384E-4 0.0 0.0 0.004666942625013189 0.0 30-31 4.0582109782723384E-4 0.0 0.0 0.010044072171224038 0.0 32-33 4.0582109782723384E-4 0.0 0.0 0.013392096228298716 0.0 34-35 4.0582109782723384E-4 0.0 0.0 0.016638665010916587 0.0 36-37 4.0582109782723384E-4 0.0 0.0 0.02232016038049786 0.0 38-39 6.087316467408508E-4 0.0 0.0 0.029726395415844882 0.0 40-41 6.087316467408508E-4 0.0 0.0 0.03449479331531488 0.0 42-43 7.101869211976592E-4 0.0 0.0 0.05194510052188593 0.0 44-45 0.0010145527445680846 0.0 0.0 0.0695983182773706 0.0 46-47 0.0011160080190248931 0.0 0.0 0.08735299130731208 0.0 48-49 0.0012174632934817016 0.0 0.0 0.10652803817964888 0.0 50-51 0.0012174632934817016 0.0 0.0 0.1344282386552712 0.0 52-53 0.0012174632934817016 0.0 0.0 0.19377957421250416 0.0 54-55 0.0012174632934817016 0.0 0.0 0.23547769201425245 0.0 56-57 0.0012174632934817016 0.0 0.0 0.31278661115034045 0.0 58-59 0.0012174632934817016 0.0 0.0 0.40582109782723386 0.0 60-61 0.0012174632934817016 0.0 0.0 0.5105229410666602 0.0 62-63 0.0012174632934817016 0.0 0.0 0.6617927552817615 0.0 64-65 0.0012174632934817016 0.0 0.0 0.7300721549911937 0.0 66-67 0.0012174632934817016 0.0 0.0 0.8949369759835074 0.0 68-69 0.0012174632934817016 0.0 0.0 1.1125585396933615 0.0 70-71 0.0012174632934817016 0.0 0.0 1.2497260707589666 0.0 72-73 0.0012174632934817016 0.0 0.0 1.5335979286891166 0.0 74-75 0.0012174632934817016 0.0 0.0 1.8100635515839198 0.0 76-77 0.0012174632934817016 0.0 0.0 1.9445932455136479 0.0 78-79 0.0012174632934817016 0.0 0.0 2.0812535002069685 0.0 80-81 0.0012174632934817016 0.0 0.0 2.386735331596419 0.0 82-83 0.0014203738423953184 0.0 0.0 2.6297207139204755 0.0 84-85 0.0015218291168521269 0.0 0.0 3.0576590615792933 0.0 86-87 0.0016232843913089354 0.0 0.0 3.6137354208770605 0.0 88-89 0.0016232843913089354 0.0 0.0 4.39828905825156 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCGTG 65 1.3274008E-4 36.53698 7 AGAATCG 70 2.0521255E-4 33.927193 5 GGGCGTA 60 0.0039495653 31.665382 9 GGTGTGC 245 0.0 27.141754 8 GAATCGT 90 8.942382E-4 26.38782 6 GTATCAA 9340 0.0 26.09099 1 ATAGACT 95 0.0012258502 24.998985 8 GAGTACT 5245 0.0 24.450867 12-13 AGTACTT 5320 0.0 24.240088 12-13 GTACTAC 100 0.0016527005 23.749035 6 GTACTTT 5595 0.0 22.878874 14-15 TAGACTA 105 0.0021949713 22.61813 5 GTACATG 4640 0.0 22.215794 1 GGTATCA 6740 0.0 22.200907 1 ATCAACG 11075 0.0 22.127783 3 TATCAAC 11050 0.0 22.091885 2 TCAACGC 11135 0.0 22.051199 4 GGTAAGG 130 2.9410332E-4 21.919962 3 CAACGCA 11245 0.0 21.837708 5 TACTTTT 5860 0.0 21.763197 14-15 >>END_MODULE