FastQCFastQC Report
Thu 26 May 2016
SRR936972_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936972_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520696
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75251.4451810653433097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53501.0274709235331172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38940.7478451918201792No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA19030.3654723677539294No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT16100.3092015302594988No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14240.2734801112357306No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12470.23948714797117707No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11320.21740132438121282No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT9890.18993808287369213No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9310.17879914575875366No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA9060.17399787976093536No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG9000.17284557592145897No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8280.15901792984774227No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC7410.14230952417533455No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG5680.10908476347043189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69650.033.1634451
GGTATCA44900.030.0619051
GTATACC856.3808385E-427.9573211
TATCAAC83850.027.5498622
ATCAACG84250.027.475483
TCAACGC85050.027.2729244
TGGACGC700.00836382127.158545
CAACGCA86000.027.0243265
AACGCAG87400.026.6976366
GGTATAG908.910835E-426.4041371
GTACATG46150.025.952151
GAGTACT44400.025.5785512-13
AGTACTT44850.025.53380812-13
GTACACG950.001221531325.0144461
TACATGG47150.024.9001222
GTCGGGA2101.2514647E-924.8977222
ACGCAGA94250.024.6540627
CGCAGAG94650.024.4494638
ACATGGG46850.024.349383
CATGGGG11300.023.9763284
Produced by FastQC (version 0.11.5)