##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936972_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 520696 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8228409667061 33.0 31.0 34.0 30.0 34.0 2 31.868854379522794 33.0 31.0 34.0 30.0 34.0 3 31.845940817674805 33.0 31.0 34.0 30.0 34.0 4 35.491519043741455 37.0 35.0 37.0 33.0 37.0 5 35.50402154039977 37.0 35.0 37.0 33.0 37.0 6 35.59220159171571 37.0 35.0 37.0 33.0 37.0 7 35.554717147817534 37.0 35.0 37.0 33.0 37.0 8 35.52271574968888 37.0 35.0 37.0 33.0 37.0 9 37.12545516001659 39.0 37.0 39.0 33.0 39.0 10-11 37.00909551830627 39.0 37.0 39.0 33.0 39.0 12-13 36.91539305083965 39.0 37.0 39.0 33.0 39.0 14-15 37.946739556286204 40.0 38.0 41.0 33.0 41.0 16-17 37.93752304607679 40.0 38.0 41.0 33.0 41.0 18-19 38.00716732988154 40.0 38.0 41.0 33.0 41.0 20-21 38.007894241553615 40.0 38.0 41.0 33.0 41.0 22-23 37.897570751455746 40.0 38.0 41.0 33.0 41.0 24-25 37.83771048750134 40.0 37.5 41.0 33.0 41.0 26-27 37.78823632215342 40.0 37.0 41.0 33.0 41.0 28-29 37.66530470754529 40.0 37.0 41.0 33.0 41.0 30-31 37.39914652695623 39.5 36.5 41.0 32.0 41.0 32-33 37.064107463856075 39.0 35.5 41.0 32.0 41.0 34-35 36.834084571419794 39.0 35.0 41.0 31.0 41.0 36-37 36.58665036796903 39.0 35.0 41.0 31.0 41.0 38-39 36.357592529998314 39.0 35.0 41.0 30.0 41.0 40-41 36.07316457203436 39.0 35.0 40.5 30.0 41.0 42-43 35.73707403168068 38.5 35.0 40.0 29.5 41.0 44-45 35.29229819318758 38.0 35.0 40.0 27.0 41.0 46-47 34.66133021955229 38.0 34.5 40.0 23.5 41.0 48-49 34.13817083288521 38.0 34.0 40.0 20.0 41.0 50-51 33.31934564505969 37.5 33.5 39.5 15.5 40.5 52-53 33.44125843102309 38.0 33.5 39.5 14.0 40.5 54-55 33.77933477499347 38.0 34.0 40.0 12.5 41.0 56-57 33.655841412263584 38.0 34.0 40.0 8.5 41.0 58-59 33.441973819656766 38.0 34.0 40.0 7.5 41.0 60-61 33.16296841151075 38.0 33.5 40.0 6.5 41.0 62-63 32.82991419177409 37.0 33.0 40.0 2.0 41.0 64-65 32.47356614992241 36.5 33.0 40.0 2.0 41.0 66-67 32.102979473627606 36.0 32.0 39.0 2.0 41.0 68-69 31.717523276537555 36.0 32.0 39.0 2.0 41.0 70-71 31.28320555564091 35.0 31.0 39.0 2.0 40.5 72-73 30.892534223424033 35.0 31.0 38.0 2.0 40.0 74-75 30.496667921397517 35.0 31.0 37.0 2.0 39.5 76-77 30.02550912624641 35.0 30.0 37.0 2.0 39.0 78-79 29.586355570236762 35.0 30.0 36.0 2.0 39.0 80-81 29.178470739164503 34.5 29.0 36.0 2.0 37.5 82-83 28.78362614654232 34.0 29.0 35.0 2.0 37.0 84-85 28.417244419008405 34.0 29.0 35.0 2.0 37.0 86-87 28.060428733848543 34.0 28.5 35.0 2.0 36.0 88-89 27.691712630786487 34.0 27.0 35.0 2.0 36.0 90-91 27.30528369720528 34.0 26.0 35.0 2.0 35.5 92-93 26.953244311460047 34.0 25.5 35.0 2.0 35.0 94-95 26.59416915052161 34.0 25.0 35.0 2.0 35.0 96-97 26.12513251494154 33.0 24.0 35.0 2.0 35.0 98-99 25.605777651451135 33.0 21.0 35.0 2.0 35.0 100-101 23.630861385530135 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2539087991976636 1101 2 0.28507665619794054 1101 3 0.28403017748918913 1101 4 0.4048889445110646 1101 5 0.3798801781556307 1101 6 0.26454862176478855 1101 7 0.1289597078392859 1101 8 0.13407130987040006 1101 9 0.1446020900342333 1101 10-11 0.2961137047150757 1101 12-13 0.38927018036257977 1101 14-15 0.5514469824653219 1101 16-17 0.5397082954188264 1101 18-19 0.41470319703758207 1101 20-21 0.4918994000637653 1101 22-23 0.39974747844799907 1101 24-25 0.40624334979126075 1101 26-27 0.4604000602930185 1101 28-29 0.5095014503290685 1101 30-31 0.5658475773787615 1101 32-33 0.5160359321721089 1101 34-35 0.49792143034267866 1101 36-37 0.4398549137016943 1101 38-39 0.38474701270848755 1101 40-41 0.6203414836122505 1101 42-43 0.6609818778220884 1101 44-45 0.7933252906442831 1101 46-47 0.7515215444635501 1101 48-49 1.0311915350439094 1101 50-51 1.1963714456680705 1101 52-53 1.0842198454704146 1101 54-55 1.055666504057335 1101 56-57 0.9288717997708673 1101 58-59 1.1592985800536155 1101 60-61 1.3528658037306158 1101 62-63 1.1994771921633003 1101 64-65 1.1251857892380812 1101 66-67 1.1952789841696791 1101 68-69 1.1678250501297605 1101 70-71 0.9773461355660267 1101 72-73 1.035430510460266 1101 74-75 1.294614680943944 1101 76-77 1.367382544775115 1101 78-79 1.361631796995308 1101 80-81 1.2745645037457116 1101 82-83 1.2662569234985384 1101 84-85 1.2130908666228635 1101 86-87 1.257777065134288 1101 88-89 1.3037866358619006 1101 90-91 1.3073461333228202 1101 92-93 1.32789703343321 1101 94-95 1.40911833385044 1101 96-97 1.2588970215466553 1101 98-99 1.0415292637665594 1101 100-101 1.258656701570228 1102 1 0.15166811093708787 1102 2 0.15969523546459996 1102 3 0.28024677373042906 1102 4 0.21521865147846597 1102 5 0.25358189808228104 1102 6 0.0773861021048603 1102 7 0.17012415544059678 1102 8 0.058609799842933796 1102 9 0.17293775477537565 1102 10-11 -0.046634952611462666 1102 12-13 0.05035294413178093 1102 14-15 0.06517088096573787 1102 16-17 0.1535655552777868 1102 18-19 -0.15279377696420227 1102 20-21 -0.010504184747539114 1102 22-23 -0.046893482333615566 1102 24-25 -0.17175764317103415 1102 26-27 -0.06861418907822525 1102 28-29 -0.05585739319602112 1102 30-31 -0.06659239189014698 1102 32-33 -0.059734519589490276 1102 34-35 0.027044398212744625 1102 36-37 0.024711514001758417 1102 38-39 0.01354128831712842 1102 40-41 -0.17873585872781206 1102 42-43 -0.1591651095883151 1102 44-45 -0.07796016064620659 1102 46-47 0.24976845867016806 1102 48-49 0.28295911663713724 1102 50-51 0.20058428749714352 1102 52-53 0.3882982387559579 1102 54-55 0.22473735952696217 1102 56-57 0.4187120560566058 1102 58-59 0.032920573081135274 1102 60-61 0.1368838220098212 1102 62-63 0.188587222532 1102 64-65 0.20892621295031688 1102 66-67 -0.030479508557053947 1102 68-69 0.39342952498317274 1102 70-71 0.45423983061788675 1102 72-73 0.4227899918005491 1102 74-75 0.46033881862505055 1102 76-77 0.4326698152884809 1102 78-79 0.5392796360339496 1102 80-81 0.7176847971406985 1102 82-83 0.6497913256555137 1102 84-85 0.5610716017048709 1102 86-87 0.5394413279141475 1102 88-89 0.6827965364931927 1102 90-91 0.31741256032560017 1102 92-93 0.43633830007620134 1102 94-95 0.8031096436184342 1102 96-97 0.54695846199429 1102 98-99 0.8653772100741186 1102 100-101 0.5676636668020514 1103 1 0.3567157974888566 1103 2 0.30386967044849555 1103 3 0.37103066348163694 1103 4 0.33672066570817094 1103 5 0.16117182065331548 1103 6 0.2036702296159163 1103 7 0.22145112458564853 1103 8 0.2509642504382299 1103 9 0.08170526152685653 1103 10-11 0.16846818133256392 1103 12-13 0.22890835114973385 1103 14-15 0.492712444079757 1103 16-17 0.5995402360963951 1103 18-19 0.444315422099109 1103 20-21 0.45457988354785783 1103 22-23 0.3809093923175979 1103 24-25 0.40187725638664773 1103 26-27 0.42037035204526063 1103 28-29 0.5108634049593235 1103 30-31 0.5641172091219104 1103 32-33 0.5022165018806746 1103 34-35 0.5058736776658037 1103 36-37 0.4155180582295799 1103 38-39 0.4486687365707098 1103 40-41 0.2729529091378282 1103 42-43 0.5934210895736953 1103 44-45 0.5871214403299589 1103 46-47 0.755485910243415 1103 48-49 0.821334902814769 1103 50-51 0.8645757819748354 1103 52-53 1.0413976403188983 1103 54-55 1.2417584977237581 1103 56-57 1.2309984086410068 1103 58-59 1.293812665995894 1103 60-61 1.2866983244636785 1103 62-63 1.0872207762791888 1103 64-65 1.2161173270122418 1103 66-67 1.2133880816346796 1103 68-69 1.1934611515297355 1103 70-71 1.0523281068140875 1103 72-73 1.0037449789256279 1103 74-75 1.060274219721439 1103 76-77 1.1808653077205413 1103 78-79 1.4600080335325352 1103 80-81 1.1691178610128645 1103 82-83 1.2671740382711398 1103 84-85 1.4083108957824102 1103 86-87 1.4225128263395455 1103 88-89 1.3886236716215379 1103 90-91 1.646523787076724 1103 92-93 1.748560726662241 1103 94-95 1.9306195801859012 1103 96-97 1.6131835532851007 1103 98-99 1.7735671398228092 1103 100-101 2.0759000270127146 1104 1 0.08708567966767689 1104 2 0.10827090994113675 1104 3 0.1480376125028755 1104 4 0.15969695116147875 1104 5 -0.0483943569225076 1104 6 0.03245663682162103 1104 7 -0.12186925882457444 1104 8 -0.09765172652940635 1104 9 0.1151842814951749 1104 10-11 0.10579113160255105 1104 12-13 0.03611637085211328 1104 14-15 -0.005310380159720296 1104 16-17 0.18357020591844986 1104 18-19 0.10895577267203294 1104 20-21 0.06971134839228199 1104 22-23 -0.20520904166670562 1104 24-25 -0.2991197142563422 1104 26-27 -0.18502988151585242 1104 28-29 0.008059515357416558 1104 30-31 -0.026379050431550866 1104 32-33 0.09975572742902727 1104 34-35 -6.576507419353561E-4 1104 36-37 0.12263155505569046 1104 38-39 0.2731365756679196 1104 40-41 0.3401927833385727 1104 42-43 0.33650097441188365 1104 44-45 0.30871254650776336 1104 46-47 0.4572792433455035 1104 48-49 0.8008520556816734 1104 50-51 0.7631674778366389 1104 52-53 0.9007146474991643 1104 54-55 0.9765842518833452 1104 56-57 0.9075451728945936 1104 58-59 0.8107776433913401 1104 60-61 0.9459202306461165 1104 62-63 0.8963626126245359 1104 64-65 1.0767437795426744 1104 66-67 0.895054112367621 1104 68-69 0.906806798852454 1104 70-71 0.7781582893848764 1104 72-73 0.79809268436113 1104 74-75 0.9681967976811308 1104 76-77 1.1312321258550604 1104 78-79 1.156657529988653 1104 80-81 1.0262481233644323 1104 82-83 1.0724410148109698 1104 84-85 1.0342488980255204 1104 86-87 1.091231824570496 1104 88-89 1.0844782509224764 1104 90-91 1.0396984838519607 1104 92-93 1.0239908491083867 1104 94-95 1.0368364104609675 1104 96-97 0.7708026174219071 1104 98-99 0.9080107556263393 1104 100-101 1.067083673109817 1105 1 0.10518771378841407 1105 2 0.09363426295950816 1105 3 -0.014101495108672424 1105 4 0.18508244984914057 1105 5 0.1365916995079388 1105 6 0.007153159131334519 1105 7 -0.0866986551500375 1105 8 -0.19035595881287293 1105 9 -0.0740422604995743 1105 10-11 0.012703451156347967 1105 12-13 0.004066666127705787 1105 14-15 0.015045180863907603 1105 16-17 0.15375967442238903 1105 18-19 0.1251180102048437 1105 20-21 0.16101917319543446 1105 22-23 0.2064256368109909 1105 24-25 0.28131007576990186 1105 26-27 0.30740573200120735 1105 28-29 0.3649451780870834 1105 30-31 0.4141516254214679 1105 32-33 0.017820852100939533 1105 34-35 -0.23510236858970046 1105 36-37 -0.0697035827395851 1105 38-39 0.057792497583932345 1105 40-41 -0.025618814430451664 1105 42-43 0.22267838582920518 1105 44-45 0.12741948548413973 1105 46-47 0.11795837720377023 1105 48-49 0.21750436867380074 1105 50-51 0.18289557823034386 1105 52-53 0.34131504119994815 1105 54-55 0.20733187327441982 1105 56-57 0.3676173513722887 1105 58-59 0.3965900613703397 1105 60-61 0.41287192027866126 1105 62-63 0.3808606441205953 1105 64-65 0.4804654822985839 1105 66-67 0.40095347260384173 1105 68-69 0.49709551276321307 1105 70-71 0.6576591916158492 1105 72-73 0.7328921430225463 1105 74-75 0.8219000457126242 1105 76-77 0.6895244486897063 1105 78-79 0.8813048396999363 1105 80-81 0.6456665944930435 1105 82-83 0.6564120613988926 1105 84-85 0.843109216266992 1105 86-87 0.8540209485862427 1105 88-89 0.8135341892426844 1105 90-91 0.5307335888388387 1105 92-93 0.5420457211556311 1105 94-95 0.7336929557365615 1105 96-97 0.5119135918313553 1105 98-99 0.847042497752323 1105 100-101 1.0139197131360653 1106 1 0.08156540196045725 1106 2 0.0853177307014441 1106 3 0.09960818231068203 1106 4 0.20581497673086346 1106 5 0.17605742531439006 1106 6 0.023719019346387427 1106 7 0.08104328033383723 1106 8 0.13835707882153514 1106 9 0.21999335777999107 1106 10-11 0.1045465291133425 1106 12-13 0.27192620913846355 1106 14-15 0.029771323337016042 1106 16-17 0.2463167980783041 1106 18-19 0.23955013386075308 1106 20-21 0.07808066513091916 1106 22-23 -0.09984116426428358 1106 24-25 -0.014859279068808462 1106 26-27 -0.13337887821384697 1106 28-29 -0.02347888105270357 1106 30-31 0.017461437249423284 1106 32-33 0.01639276070309137 1106 34-35 0.20170878732427155 1106 36-37 0.13222854091632996 1106 38-39 -0.054346279297789124 1106 40-41 0.016308268902882617 1106 42-43 0.10860748529156439 1106 44-45 0.5666415956991955 1106 46-47 0.4876492374188288 1106 48-49 0.03503629071681047 1106 50-51 0.2835256185529218 1106 52-53 0.2045542885117797 1106 54-55 0.396688392091626 1106 56-57 0.14678401803895014 1106 58-59 0.1114338113703397 1106 60-61 0.207134353074359 1106 62-63 0.430390214013066 1106 64-65 0.126777646277084 1106 66-67 0.38404319034578194 1106 68-69 0.16161164179547072 1106 70-71 0.37147807602445226 1106 72-73 0.4819932854956619 1106 74-75 0.7207071693685378 1106 76-77 0.7227569755714249 1106 78-79 0.669040054753701 1106 80-81 0.7731027504070198 1106 82-83 0.6970790640870668 1106 84-85 0.39740189099817513 1106 86-87 0.6298526018120505 1106 88-89 0.7875093236512853 1106 90-91 0.7895037501291604 1106 92-93 0.8995306001878873 1106 94-95 0.9823236681021505 1106 96-97 1.4287062665625392 1106 98-99 1.5648264359243669 1106 100-101 1.6018817427059595 1107 1 0.13989349373270343 1107 2 0.11509578802914788 1107 3 0.182904075921968 1107 4 -0.00925076742105091 1107 5 0.05061938056643811 1107 6 0.04245086108119267 1107 7 -0.08001341838123466 1107 8 0.11559372781665189 1107 9 0.23671244005753067 1107 10-11 0.33300071786944585 1107 12-13 0.2812472023394079 1107 14-15 0.27276410495861114 1107 16-17 0.3433096396034472 1107 18-19 0.5903034210683415 1107 20-21 0.3578093020255224 1107 22-23 0.6310121618806264 1107 24-25 0.9450113292517983 1107 26-27 0.7868864945360272 1107 28-29 0.6887743911790025 1107 30-31 0.46776844297021114 1107 32-33 0.6442179621287991 1107 34-35 0.6928790032765946 1107 36-37 0.3559623295166858 1107 38-39 0.5431707183360146 1107 40-41 0.47433983807651003 1107 42-43 -0.03822211904545014 1107 44-45 0.09261577726687165 1107 46-47 -0.010610564300407077 1107 48-49 0.2021252774481752 1107 50-51 0.2865537432721297 1107 52-53 0.6887906679408999 1107 54-55 0.4371173015473957 1107 56-57 0.22635690568983335 1107 58-59 0.6359463461892787 1107 60-61 0.6019017775210003 1107 62-63 0.8786844602765811 1107 64-65 0.9883585011008122 1107 66-67 0.6848518883419992 1107 68-69 0.6569840921503989 1107 70-71 0.5432245641785229 1107 72-73 0.9066016660030485 1107 74-75 0.6736888688324036 1107 76-77 0.5851285573248042 1107 78-79 0.6000461349645612 1107 80-81 0.6715153061922052 1107 82-83 0.8702243002568295 1107 84-85 0.8278868102781338 1107 86-87 1.0345071080569959 1107 88-89 0.680147176707866 1107 90-91 0.7623404829892557 1107 92-93 1.2918389836904502 1107 94-95 1.2318279661822409 1107 96-97 0.9811967568731355 1107 98-99 0.9799790402314308 1107 100-101 1.234613045308766 1108 1 -0.0041264589609788516 1108 2 0.1041231619862728 1108 3 0.24386687472101087 1108 4 0.04286073452067285 1108 5 0.023008214106482683 1108 6 0.14640954599264688 1108 7 0.07534514047040375 1108 8 -0.05503433472528485 1108 9 -0.14547246122950241 1108 10-11 -0.1726923061429133 1108 12-13 -0.08996846160952288 1108 14-15 -0.10162338665434589 1108 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100-101 -2.3039288445562427 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 942.0 3 316.0 4 318.0 5 391.0 6 390.0 7 519.0 8 657.0 9 809.0 10 1169.0 11 1660.0 12 2488.0 13 3321.0 14 3835.0 15 5188.0 16 7182.0 17 10310.0 18 8958.0 19 4304.0 20 3844.0 21 3779.0 22 3827.0 23 4185.0 24 4662.0 25 5189.0 26 5967.0 27 6662.0 28 7818.0 29 9205.0 30 11227.0 31 14031.0 32 17597.0 33 22976.0 34 31764.0 35 46892.0 36 66344.0 37 94954.0 38 89352.0 39 17652.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.9592291582608 18.803965646434953 15.31058466386151 19.926220531442734 2 14.990673093363199 21.15084998760895 41.52384940935995 22.3346275096679 3 18.779669222390215 28.326843165920955 31.158951147962373 21.734536463726464 4 11.592849442839931 17.30030533793742 38.822690701700786 32.28415451752186 5 12.04775499610693 38.711345656583134 33.48098163047553 15.759917716834405 6 27.821354742560455 37.57992404211336 18.61564347867891 15.983077736647278 7 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34.866649508890674 20.00555066857707 30-31 19.680717233855805 26.000207426243644 34.61194967224733 19.707125667653223 32-33 19.24609005126196 26.10691450642359 34.14451685268093 20.502478589633522 34-35 19.747242669125097 25.83272112665236 34.085843380725336 20.33419282349721 36-37 20.151805717874527 25.685396862284904 33.99687515304932 20.16592226679125 38-39 20.22370917806851 25.79387181279721 33.948079035715104 20.034339973419172 40-41 19.79314715937464 25.922002842546 34.08519955441171 20.199650443667654 42-43 19.55644754720967 26.521824720439042 33.41018759032616 20.51154014202513 44-45 19.70462129396231 26.8715880446525 32.74545672788381 20.67833393350138 46-47 19.951679548599408 26.85501271868797 31.805842434229163 21.38746529848346 48-49 20.223028408130656 27.239598537342324 30.995724952755545 21.541648101771475 50-51 20.733161766118037 27.680097562849298 30.5225758128637 21.06416485816897 52-53 20.428488430716342 27.98234958010631 30.40404864908987 21.185113340087483 54-55 19.837910098590765 27.74236176068931 30.767214008992834 21.652514131727095 56-57 20.465931887783764 28.367701868125856 29.618817048893177 21.547549195197206 58-59 20.45477898210096 27.690833262111063 30.16101353927292 21.693374216515053 60-61 20.55672708695836 27.353241685007855 30.621910325993017 21.46812090204077 62-63 21.271132076196476 27.20367076791881 30.397442447383483 21.127754708501232 64-65 20.900140295821565 27.452656161925 30.675811022200538 20.97139252005289 66-67 20.55631965761398 27.781790380766143 30.29215378172891 21.369736179890968 68-69 20.54128883692646 28.03300532316031 30.387934554236963 21.03777128567627 70-71 20.35856455036267 28.081663321358118 29.895336201106694 21.66443592717252 72-73 20.463009020878424 27.99189907064018 29.506646103852457 22.038445804628935 74-75 20.423103998340466 28.038514987174157 29.86947506715441 21.668905947330966 76-77 20.59089918062467 27.836766526534372 29.916852277125784 21.65548201571518 78-79 20.220456165223023 27.76429862627916 30.099489585194323 21.9157556233035 80-81 20.073557748214157 28.599875257437702 29.699973955905712 21.62659303844243 82-83 20.070580483170858 28.38828102712469 29.4263366635257 22.114801826178752 84-85 20.10498727994399 27.93322378751312 29.09075368612722 22.871035246415673 86-87 20.30222809653164 27.817203102967337 29.398752050778626 22.481816749722398 88-89 20.54838870827665 27.16215904636739 29.472746766272394 22.816705479083566 90-91 19.849542151637532 27.43891192327053 30.215494218775817 22.496051706316116 92-93 19.886679674103195 27.78240499239632 29.728140733930676 22.602774599569813 94-95 19.564223619663718 27.96849610282847 29.579992854865417 22.887287422642395 96-97 19.37843112396802 27.865528753251045 29.371477635506587 23.384562487274348 98-99 19.51824034953225 27.971706179258245 29.250365537104177 23.259687934105333 100-101 19.806955966815572 28.312195815809503 29.099807013632066 22.78104120374286 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 770.0 1 546.0 2 415.0 3 504.0 4 432.0 5 440.5 6 773.0 7 1697.0 8 2867.5 9 3205.5 10 2531.0 11 1947.5 12 1894.0 13 1972.0 14 2049.5 15 2031.0 16 1976.5 17 1982.5 18 2018.0 19 2119.5 20 2283.5 21 2427.0 22 2712.5 23 3060.0 24 3392.0 25 3956.0 26 4567.0 27 5300.5 28 5920.5 29 6513.5 30 7366.5 31 8096.0 32 9062.5 33 10202.0 34 11360.5 35 12187.5 36 13010.0 37 14076.5 38 14507.5 39 14378.0 40 14342.5 41 14725.0 42 15070.5 43 15409.5 44 16060.5 45 16730.0 46 16916.5 47 17655.5 48 23961.0 49 24072.0 50 17583.5 51 15586.0 52 14187.0 53 13778.0 54 13382.5 55 12401.0 56 11286.5 57 9993.0 58 9194.5 59 8485.5 60 7592.5 61 6670.5 62 5511.0 63 4010.5 64 2700.5 65 1980.0 66 1419.0 67 1087.5 68 782.5 69 476.5 70 335.0 71 260.0 72 223.0 73 202.5 74 148.5 75 99.5 76 74.5 77 42.5 78 22.0 79 17.0 80 12.5 81 12.0 82 9.0 83 3.0 84 2.5 85 3.0 86 2.5 87 3.0 88 2.0 89 0.5 90 0.0 91 1.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043403444620277475 2 0.030536051746124417 3 0.03168835558560081 4 0.0555026349347796 5 0.10389939619278811 6 0.12886597938144329 7 0.06606542012997987 8 0.10044248467435894 9 0.08527048412125308 10-11 0.0832539524021694 12-13 0.08094934472321662 14-15 0.07374744572648917 16-17 0.05953569837294698 18-19 0.06692964800958717 20-21 0.060591976892467 22-23 0.035145267104029994 24-25 0.009122405395854778 26-27 0.003744987478298278 28-29 0.007297924316683823 30-31 0.005569468557469233 32-33 0.007105873676771091 34-35 0.007393949636640189 36-37 0.005857544517338332 38-39 0.0038410127982546434 40-41 0.006913823036858359 42-43 0.0025926836388218844 44-45 0.0016324304392582238 46-47 0.001056278519520027 48-49 0.0 50-51 0.0011523038394763933 52-53 0.005473443237512868 54-55 0.0113309877548512 56-57 0.007778050916465654 58-59 0.004897291317774671 60-61 0.0019205063991273217 62-63 0.008066126876334753 64-65 0.0020165317190836882 66-67 0.02189377295005147 68-69 0.0450358750595357 70-71 0.060591976892467 72-73 0.0028807595986909827 74-75 0.012963418194109422 76-77 0.0223738995498333 78-79 0.026695038947869773 80-81 0.08152549664295482 82-83 0.029863874506429855 84-85 0.011811114354633029 86-87 0.031112203665862617 88-89 0.046860356138706656 90-91 0.04186703950097562 92-93 0.043115368660408375 94-95 0.011907139674589397 96-97 0.018052760151796826 98-99 0.043979596540015674 100-101 0.08680688924055495 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 520696.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.3122526860016 #Duplication Level Percentage of deduplicated Percentage of total 1 82.64813322801479 51.499913617303825 2 10.563985239399408 13.165314352172942 3 3.0024106135568474 5.612609064572621 4 1.2535983553528227 3.1245814994200445 5 0.642235949385333 2.00095843810665 6 0.3996940142238619 1.4943500646839762 7 0.25874952361026904 1.1286285988309934 8 0.19996735300362214 0.9968332983450068 9 0.14585714936311026 0.8179818792456603 >10 0.8066010717427564 9.234676709226806 >50 0.048614318656916684 2.054725426511572 >100 0.024922529376687676 2.9301453412009355 >500 0.002769169930743059 1.3215659122487722 >1k 0.0018461132871620395 2.1492461738442965 >5k 6.153710957206798E-4 2.4684696242859334 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7525 1.4451810653433097 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5350 1.0274709235331172 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3894 0.7478451918201792 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1903 0.3654723677539294 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1610 0.3092015302594988 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1424 0.2734801112357306 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1247 0.23948714797117707 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1132 0.21740132438121282 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 989 0.18993808287369213 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 931 0.17879914575875366 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 906 0.17399787976093536 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 900 0.17284557592145897 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 828 0.15901792984774227 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 741 0.14230952417533455 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 568 0.10908476347043189 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 1.9205063991273218E-4 0.0 7 0.0 0.0 0.0 1.9205063991273218E-4 0.0 8 0.0 0.0 0.0 1.9205063991273218E-4 0.0 9 0.0 0.0 0.0 1.9205063991273218E-4 0.0 10-11 0.0 0.0 0.0 1.9205063991273218E-4 0.0 12-13 0.0 0.0 0.0 1.9205063991273218E-4 0.0 14-15 1.9205063991273218E-4 0.0 0.0 1.9205063991273218E-4 0.0 16-17 1.9205063991273218E-4 0.0 0.0 1.9205063991273218E-4 0.0 18-19 2.8807595986909826E-4 0.0 0.0 1.9205063991273218E-4 0.0 20-21 3.8410127982546437E-4 0.0 0.0 1.9205063991273218E-4 0.0 22-23 3.8410127982546437E-4 0.0 0.0 3.8410127982546437E-4 0.0 24-25 5.761519197381965E-4 0.0 0.0 9.60253199563661E-4 0.0 26-27 5.761519197381965E-4 0.0 0.0 0.0014403797993454914 0.0 28-29 7.682025596509287E-4 0.0 0.0 0.0027847342787346166 0.0 30-31 7.682025596509287E-4 0.0 0.0 0.00614562047720743 0.0 32-33 7.682025596509287E-4 0.0 0.0 0.009218430715811144 0.0 34-35 7.682025596509287E-4 0.0 0.0 0.014019696713629449 0.0 36-37 7.682025596509287E-4 0.0 0.0 0.019301089311229584 0.0 38-39 7.682025596509287E-4 0.0 0.0 0.02410235530904789 0.0 40-41 7.682025596509287E-4 0.0 0.0 0.02851952002704073 0.0 42-43 7.682025596509287E-4 0.0 0.0 0.04493984973957933 0.0 44-45 9.60253199563661E-4 0.0 0.0 0.06424093905080892 0.0 46-47 9.60253199563661E-4 0.0 0.0 0.07912486364404567 0.0 48-49 9.60253199563661E-4 0.0 0.0 0.1016908138337917 0.0 50-51 9.60253199563661E-4 0.0 0.0 0.13145866302026518 0.0 52-53 0.001056278519520027 0.0 0.0 0.194355247591685 0.0 54-55 0.001152303839476393 9.602531995636609E-5 0.0 0.23670241369244244 0.0 56-57 0.001152303839476393 1.9205063991273218E-4 0.0 0.31044985941893155 0.0 58-59 0.001152303839476393 1.9205063991273218E-4 0.0 0.40004148293822117 0.0 60-61 0.001152303839476393 1.9205063991273218E-4 0.0 0.49280194201607075 0.0 62-63 0.001152303839476393 1.9205063991273218E-4 0.0 0.63683992195062 0.0 64-65 0.001152303839476393 1.9205063991273218E-4 0.0 0.6979120254428688 0.0 66-67 0.001152303839476393 1.9205063991273218E-4 0.0 0.8509763854533163 0.0 68-69 0.001152303839476393 1.9205063991273218E-4 0.0 1.0472521394441285 0.0 70-71 0.0015364051193018575 1.9205063991273218E-4 0.0 1.1752538909459647 0.0 72-73 0.0017284557592145897 1.9205063991273218E-4 0.0 1.4309693179897676 0.0 74-75 0.0017284557592145897 1.9205063991273218E-4 0.0 1.66978428872125 0.0 76-77 0.0017284557592145897 1.9205063991273218E-4 0.0 1.7897199133467514 0.0 78-79 0.0017284557592145897 1.9205063991273218E-4 0.0 1.92002627252754 0.0 80-81 0.002304607678952786 1.9205063991273218E-4 0.0 2.1974434218814816 0.0 82-83 0.002304607678952786 1.9205063991273218E-4 0.0 2.408987201745356 0.0 84-85 0.0024966583188655183 1.9205063991273218E-4 0.0 2.7913600258116062 0.0 86-87 0.0026887089587782505 1.9205063991273218E-4 0.0 3.341393058521671 0.0 88-89 0.0026887089587782505 1.9205063991273218E-4 0.0 4.098168605097792 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6965 0.0 33.163445 1 GGTATCA 4490 0.0 30.061905 1 GTATACC 85 6.3808385E-4 27.957321 1 TATCAAC 8385 0.0 27.549862 2 ATCAACG 8425 0.0 27.47548 3 TCAACGC 8505 0.0 27.272924 4 TGGACGC 70 0.008363821 27.15854 5 CAACGCA 8600 0.0 27.024326 5 AACGCAG 8740 0.0 26.697636 6 GGTATAG 90 8.910835E-4 26.404137 1 GTACATG 4615 0.0 25.95215 1 GAGTACT 4440 0.0 25.57855 12-13 AGTACTT 4485 0.0 25.533808 12-13 GTACACG 95 0.0012215313 25.014446 1 TACATGG 4715 0.0 24.900122 2 GTCGGGA 210 1.2514647E-9 24.897722 2 ACGCAGA 9425 0.0 24.654062 7 CGCAGAG 9465 0.0 24.449463 8 ACATGGG 4685 0.0 24.34938 3 CATGGGG 1130 0.0 23.976328 4 >>END_MODULE