FastQCFastQC Report
Thu 26 May 2016
SRR936972_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936972_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520696
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79631.5292992456250865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60741.1665155868299353No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43980.8446387143361961No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA27440.5269869559205371No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18700.3591346966368092No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14320.2750165163550325No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13120.25197043956550463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11970.22988461597554044No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11750.2256595018974603No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT11400.21893772950051468No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG8470.16266689200608417No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA7730.148455144652542No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG7420.14250157481524728No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT7000.13443544793891252No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6810.13078648578057062No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6580.12636932106257778No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT6080.11676678906694117No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC5210.10005838339453346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAA300.00395211331.6644960-61
CTTAACG650.00583765429.2343739
GTATCAA88050.026.9278561
GGTATCA62650.024.3452681
AGTACTT56000.023.79420512-13
GAGTACT55600.023.15372312-13
GTACATG46400.022.938271
CGGGTCG1252.2531857E-422.8006216
GCACTTA1252.2531857E-422.8006216
CGGTTAC853.1503932E-622.35140428-29
ATCAACG106500.022.2097423
TACCTGG3650.022.1238922
TACATGG48200.022.0752932
TATCAAC107850.021.9778822
TCAACGC107700.021.9181774
CAACGCA109150.021.6270075
GTGTAGC2005.3581425E-721.3817441
GTACTTT60400.021.3126714-15
AACGCAG111850.021.106976
ACATGGG48750.020.9472773
Produced by FastQC (version 0.11.5)